Atsushi Miyawaki

发表

Nanoaquarium: integrated microchips fabricated by ultrafast laser for understanding phenomena and functions of microorganisms

Koji Sugioka, Katsumi Midorikawa, Yasutaka Hanada, 2011, Other Conferences.

On-chip manipulation and sensing of microorganisms by magnetically driven microtools with a force sensing structure

Fumihito Arai, Yoko Yamanishi, Tomohiro Kawahara, 2012, 2012 IEEE International Conference on Robotics and Automation.

Adaptive control of two-photon fluorescence from green fluorescent protein by shaped femtosecond excitation pulses

Katsumi Midorikawa, Hiroyuki Kawano, Atsushi Miyawaki, 2003, European Conference on Biomedical Optics.

Live Imaging-Based Model Selection Reveals Periodic Regulation of the Stochastic G1/S Phase Transition in Vertebrate Axial Development

Takeshi Imamura, Atsushi Miyawaki, Takashi Saitou, 2014, PLoS Comput. Biol..

Voices of biotech

Jun Qin, Bonnie Berger, Drew Endy, 2016, Nature Biotechnology.

Extending Whole Slide Imaging: Color Darkfield Internal Reflection Illumination (DIRI) for Biological Applications

Takuji Ishikawa, Yoshihiro Kawano, Atsushi Miyawaki, 2017, PloS one.

1A2-B09 Biotope on a Chip : Mechanical Stimulation of P. laevis by Microrobot(Nano/Micro Fluid System)

Fumihito Arai, Yoko Yamanishi, Tomohiro Kawahara, 2011 .

Whole-Brain Analysis of Cells and Circuits by Tissue Clearing and Light-Sheet Microscopy

Kwanghun Chung, Hans-Ulrich Dodt, Atsushi Miyawaki, 2018, The Journal of Neuroscience.

A spherical aberration-free microscopy system for live brain imaging.

Atsushi Miyawaki, Hajime Hirase, Yoshihiro Ue, 2018, Biochemical and biophysical research communications.

3D microstructuring inside glass by ultrafast laser

Koji Sugioka, Katsumi Midorikawa, Yasutaka Hanada, 2011, Other Conferences.

Recent advances in 3D printing of pure proteinaceous microstructures by femtosecond laser direct write

Koji Sugioka, Atsushi Miyawaki, Hiroyuki Kawano, 2020, LASE.

Nano-aquarium for dynamic observation of aquatic microorganisms fabricated by femtosecond laser direct writing of photostructurable glass

Koji Sugioka, Katsumi Midorikawa, Yasutaka Hanada, 2008, SPIE LASE.

Phase-Locked Signals Elucidate Circuit Architecture of an Oscillatory Pathway

Shuichi Takayama, Richard R. Neubig, Atsushi Miyawaki, 2010, PLoS Comput. Biol..

On-chip microrobot for investigating the response of aquatic microorganisms to mechanical stimulation.

Fumihito Arai, Hiroyuki Kawano, Atsushi Miyawaki, 2013, Lab on a chip.

Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain

Atsushi Miyawaki, Asako Sakaue-Sawano, Hiroyuki Kawano, 2011, Nature Neuroscience.

An Ultra-high-speed and Ultra-high-sensitivity Video Camera and its Applications to Bioscience

Shigeru Yoshida, Atsushi Miyawaki, Hiroyuki Kawano, 2009 .

Coherent control of mutiphoton excitation process for biological fluorescence imaging

Katsumi Midorikawa, Atsushi Miyawaki, Hideaki Mizuno, 2005, SPIE LASE.

On-chip force sensing by magnetically driven microtool for measurement of stimulant property of P. laevis

Fumihito Arai, Yoko Yamanishi, Tomohiro Kawahara, 2011, 2011 IEEE International Conference on Robotics and Automation.

Cross-talk-free fluorescence cross-correlation spectroscopy by the switching method

Yasuo Takahashi, Masataka Kinjo, Atsushi Miyawaki, 2007, SPIE BiOS.

Ultrafast dynamics in a live cell irradiated by femtosecond laser pulses

Takeharu Etoh, Atsushi Miyawaki, Hiroyuki Kawano, 2007, European Conference on Biomedical Optics.

Development of an optical coherence tomographic system for imaging of tissue

Atsushi Miyawaki, Jiyuan Liu, 1999, Other Conferences.

Femtosecond Laser Direct Write Integration of Multi-Protein Patterns and 3D Microstructures into 3D Glass Microfluidic Devices

Koji Sugioka, Katsumi Midorikawa, Hiroyuki Kawano, 2018 .

Genetically Encoded Tools for Optical Dissection of the Mammalian Cell Cycle.

Atsushi Miyawaki, Hiroyuki Miyoshi, Michiyuki Matsuda, 2017, Molecular cell.

Brain/MINDS: brain-mapping project in Japan

Hideyuki Okano, Atsushi Miyawaki, Kiyoto Kasai, 2015, Philosophical Transactions of the Royal Society B: Biological Sciences.

Spatio-temporal activation of caspase revealed by indicator that is insensitive to environmental effects

Takeharu Nagai, Masayuki Miura, Atsushi Miyawaki, 2003, The Journal of cell biology.

Green fluorescent protein-like proteins in reef Anthozoa animals.

Atsushi Miyawaki, A. Miyawaki, 2002, Cell structure and function.

Illuminating cell-cycle progression in the developing zebrafish embryo

Atsushi Miyawaki, Hitoshi Okamoto, Mayu Sugiyama, 2009, Proceedings of the National Academy of Sciences.

Fluorescent proteins in a new light

Atsushi Miyawaki, A. Miyawaki, 2004, Nature Biotechnology.

Attractive axon guidance involves asymmetric membrane transport and exocytosis in the growth cone

Atsushi Miyawaki, Yan Li, Hiroyuki Kamiguchi, 2007, Nature Neuroscience.

Colored Fluorescent Silk Made by Transgenic Silkworms

Kenichi Nakajima, Atsushi Miyawaki, Hideki Sezutsu, 2013 .

New technologies for neuroscience

Atsushi Miyawaki, Mark J Schnitzer, 2007, Current Opinion in Neurobiology.

Background-free deep imaging by spatial overlap modulation nonlinear optical microscopy

Katsumi Midorikawa, Atsushi Miyawaki, Hideaki Mizuno, 2012, Biomedical optics express.

Monitoring cellular movement in vivo with photoconvertible fluorescence protein “Kaede” transgenic mice

Atsushi Miyawaki, Junko Tanaka, Osami Kanagawa, 2008, Proceedings of the National Academy of Sciences.

Tracing the silhouette of individual cells in S/G2/M phases with fluorescence.

Atsushi Miyawaki, Masako Kawano, Asako Sakaue-Sawano, 2008, Chemistry & biology.

Brain clearing for connectomics.

Atsushi Miyawaki, A. Miyawaki, 2015, Microscopy.

Whole-field fluorescence microscope with digital micromirror device: imaging of biological samples.

Atsushi Miyawaki, Takashi Fukano, A. Miyawaki, 2003, Applied optics.

Single-cell bioluminescence imaging of deep tissue in freely moving animals

Hideyuki Okano, Thomas J. McHugh, Atsushi Miyawaki, 2018, Science.

Resource Whole-Brain Imaging with Single-Cell Resolution Using Chemical Cocktails and Computational Analysis

Takehiro Tawara, Hideo Yokota, Chihiro Yokoyama, 2014 .

[Visualizing spatiotemporal dynamics of multicellular cell-cycle progression].

Atsushi Miyawaki, Asako Sakaue-Sawano, A. Miyawaki, 2012, Seikagaku. The Journal of Japanese Biochemical Society.

Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein

Takeharu Nagai, Atsushi Miyawaki, Tomoki Matsuda, 2008, Nature Methods.

A theoretical study on the nature of on- and off-states of reversibly photoswitching fluorescent protein Dronpa: absorption, emission, protonation, and Raman.

Xin Li, Atsushi Miyawaki, Hideaki Mizuno, 2010, The journal of physical chemistry. B.

Crystal Structure of Venus, a Yellow Fluorescent Protein with Improved Maturation and Reduced Environmental Sensitivity*

Takeharu Nagai, Atsushi Miyawaki, Mitsuhiko Ikura, 2002, The Journal of Biological Chemistry.

FRET-based in vivo Ca2+ imaging by a new calmodulin-GFP fusion molecule

Kevin Truong, Atsushi Miyawaki, Hideaki Mizuno, 2001, Nature Structural Biology.

Second-harmonic generation in GFP-like proteins.

Atsushi Miyawaki, Inge Asselberghs, Hideaki Mizuno, 2008, Journal of the American Chemical Society.

Visualizing spatiotemporal dynamics of multicellular cell-cycle progressions with fucci technology.

Atsushi Miyawaki, Asako Sakaue-Sawano, A. Miyawaki, 2014, Cold Spring Harbor protocols.

Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy.

Katsumi Midorikawa, Fumihiko Kannari, Atsushi Miyawaki, 2010, Applied optics.

Lateral propagation of EGF signaling after local stimulation is dependent on receptor density.

Shuichi Takayama, Atsushi Miyawaki, Michiyuki Matsuda, 2002, Developmental cell.

mKikGR, a Monomeric Photoswitchable Fluorescent Protein

Atsushi Miyawaki, Antoine M. van Oijen, A. Miyawaki, 2008, PloS one.

Local generation of glia is a major astrocyte source in postnatal cortex

Fred H. Gage, Atsushi Miyawaki, Yuh Nung Jan, 2012, Nature.

Excited states of fluorescent proteins, mKO and DsRed: chromophore-protein electrostatic interaction behind the color variations.

Atsushi Miyawaki, Akihiro Kikuchi, Yoshitsugu Shiro, 2010, The journal of physical chemistry. B.

Fluorescence imaging using a fluorescent protein with a large Stokes shift.

Atsushi Miyawaki, Hiroyuki Kawano, A. Miyawaki, 2008, Methods.

Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching

Peter Dedecker, Atsushi Miyawaki, Hideaki Mizuno, 2006, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

Development of microscopic systems for high-speed dual-excitation ratiometric Ca2+ imaging

Atsushi Miyawaki, Takashi Fukano, A. Miyawaki, 2008, Brain cell biology.

Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter

Atsushi Miyawaki, Toshitaka Horiuchi, Asako Sakaue-Sawano, 2013, Development.

Red fluorescent proteins: chromophore formation and cellular applications.

Atsushi Miyawaki, Vladislav V Verkhusha, A. Miyawaki, 2012, Current opinion in structural biology.

Real-time tracking of cell cycle progression during CD8+ effector and memory T-cell differentiation

William Ritchie, Philip D. Hodgkin, Atsushi Miyawaki, 2015, Nature Communications.

A stroboscopic approach for fast photoactivation-localization microscopy with Dronpa mutants.

Peter Dedecker, Atsushi Miyawaki, Hideaki Mizuno, 2007, Journal of the American Chemical Society.

Green Fluorescent Protein Glows Gold

Atsushi Miyawaki, A. Miyawaki, 2008, Cell.

Primary structure and functional expression of the inositol 1,4,5-trisphosphate-binding protein P400

Teiichi Furuichi, Atsushi Miyawaki, Shingo Yoshikawa, 1989, Nature.

Innovations in the Imaging of Brain Functions using Fluorescent Proteins

Atsushi Miyawaki, A. Miyawaki, 2005, Neuron.

Lighting up cells: labelling proteins with fluorophores.

Atsushi Miyawaki, A. Miyawaki, A. Sawano, 2003, Nature cell biology.

Circularly permuted green fluorescent proteins engineered to sense Ca 2 + and their application to imaging of subcellular Ca 2 + dynamics

Takeharu Nagai, Atsushi Miyawaki, Hideaki Mizuno, 2002 .

Subdiffraction imaging through the selective donut-mode depletion of thermally stable photoswitchable fluorophores: numerical analysis and application to the fluorescent protein Dronpa.

Peter Dedecker, Atsushi Miyawaki, Hideaki Mizuno, 2007, Journal of the American Chemical Society.

Development of cell-impermeable coelenterazine derivatives

Atsushi Miyawaki, Kazuya Kikuchi, Takashi Fukano, 2013 .

Quantitative Comparison of Genetically Encoded Ca2+ Indicators in Cortical Pyramidal Cells and Cerebellar Purkinje Cells

Takeharu Nagai, Atsushi Miyawaki, Michael Häusser, 2011, Front. Cell. Neurosci..

Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy.

Katsumi Midorikawa, Fumihiko Kannari, Atsushi Miyawaki, 2007, Applied Optics.

Electrical Stimulation Modulates Fate Determination of Differentiating Embryonic Stem Cells

Akihiko Takashima, Hideyuki Okano, Atsushi Miyawaki, 2007, Stem cells.

Fast and reversible photoswitching of the fluorescent protein dronpa as evidenced by fluorescence correlation spectroscopy.

Peter Dedecker, Atsushi Miyawaki, Johan Hofkens, 2006, Biophysical journal.

Moments reconstruction and local dynamic range compression of high order Superresolution Optical Fluctuation Imaging

Shimon Weiss, Atsushi Miyawaki, Xiyu Yi, 2018 .

PKC Signaling Mediates Global Enhancement of Excitatory Synaptogenesis in Neurons Triggered by Local Contact with Astrocytes

Atsushi Miyawaki, Hiroshi Hama, Kazuhiko Yamaguchi, 2004, Neuron.

A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy

Atsushi Miyawaki, Satoshi Karasawa, Kenta Saito, 2006, Nature Biotechnology.

Fucci2a: A bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice

Atsushi Miyawaki, Asako Sakaue-Sawano, Peter Hohenstein, 2014, Cell cycle.

Contrasting Quiescent G0 Phase with Mitotic Cell Cycling in the Mouse Immune System

Atsushi Miyawaki, Asako Sakaue-Sawano, Osami Kanagawa, 2013, PloS one.

Supplemental materials and methods

Manabu Yoshida, Atsushi Miyawaki, Takafumi Inoue, 2006 .

Improved detection of electrical activity with a voltage probe based on a voltage‐sensing phosphatase

Yasushi Okamura, Atsushi Miyawaki, Katsuhiko Mikoshiba, 2013, The Journal of physiology.

Fast dual-excitation ratiometry with light-emitting diodes and high-speed liquid crystal shutters.

Atsushi Miyawaki, Takashi Fukano, A. Miyawaki, 2006, Biochemical and biophysical research communications.

Engineering fluorescent proteins.

Takeharu Nagai, Atsushi Miyawaki, Hideaki Mizuno, 2005, Advances in biochemical engineering/biotechnology.

An efficient gene delivery system into cells using a nano-size silicon needle

Hideyuki Okano, Atsushi Miyawaki, Chikako Hara, 2007, Neuroscience Research.

Visualizing voltage dynamics in zebrafish heart

Yasushi Okamura, Atsushi Miyawaki, S. Higashijima, 2010, The Journal of physiology.

Local apoptosis modulates early mammalian brain development through the elimination of morphogen-producing cells.

Keisuke Kuida, Masayuki Miura, Atsushi Miyawaki, 2013, Developmental cell.

Semi‐rational engineering of a coral fluorescent protein into an efficient highlighter

Atsushi Miyawaki, Satoshi Karasawa, Nobuyuki Nukina, 2005, EMBO reports.

Temporal focusing microscopy using three-photon excitation fluorescence with a 92-fs Yb-fiber chirped pulse amplifier.

Katsumi Midorikawa, Atsushi Miyawaki, Keisuke Isobe, 2017, Biomedical optics express.

A practical device for pinpoint delivery of molecules into multiple neurons in culture

Hideyuki Okano, Atsushi Miyawaki, Hiroyuki Imabayashi, 2006, Brain cell biology.

Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET Control

Takeharu Nagai, Thomas Euler, Atsushi Miyawaki, 2004, PLoS biology.

Rapid and persistent modulation of actin dynamics regulates postsynaptic reorganization underlying bidirectional plasticity

Takeharu Nagai, Atsushi Miyawaki, Yasunori Hayashi, 2004, Nature Neuroscience.

In vitro characterization of the human segmentation clock

Jonathan Touboul, Kumiko Yoshioka-Kobayashi, Ryoichiro Kageyama, 2018, bioRxiv.

Molecular spies for bioimaging--fluorescent protein-based probes.

Atsushi Miyawaki, A. Miyawaki, Y. Niino, 2015, Molecular cell.

Improving membrane voltage measurements using FRET with new fluorescent proteins

Yasushi Okamura, Atsushi Miyawaki, Satoshi Karasawa, 2008, Nature Methods.

A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery.

Atsushi Miyawaki, A. Miyawaki, N. Mizushima, 2011, Chemistry & biology.

Fourier-transform spectroscopy combined with a 5-fs broadband pulse for multispectral nonlinear microscopy

Katsumi Midorikawa, Fumihiko Kannari, Atsushi Miyawaki, 2008 .

Fluorescence imaging of physiological activity in complex systems using GFP-based probes

Atsushi Miyawaki, A. Miyawaki, 2003, Current Opinion in Neurobiology.

Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein.

Atsushi Miyawaki, Hideaki Mizuno, Mitsuhiko Ikura, 2003, Molecular cell.

Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano

Takeharu Nagai, Atsushi Miyawaki, Tomoki Matsuda, 2010, Nature Methods.

Attenuation of photobleaching in two-photon excitation fluorescence from green fluorescent protein with shaped excitation pulses.

Katsumi Midorikawa, Atsushi Miyawaki, Hideaki Mizuno, 2003, Biochemical and biophysical research communications.

Enhancement of lateral resolution and optical sectioning capability of two-photon fluorescence microscopy by combining temporal-focusing with structured illumination.

Katsumi Midorikawa, Fumihiko Kannari, Atsushi Miyawaki, 2013, Biomedical optics express.

Development of probes for cellular functions using fluorescent proteins and fluorescence resonance energy transfer.

Atsushi Miyawaki, A. Miyawaki, 2011, Annual review of biochemistry.

ScaleS: an optical clearing palette for biological imaging

Atsushi Miyawaki, Takeshi Kaneko, Hiroshi Kurokawa, 2015, Nature Neuroscience.

Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression

Atsushi Miyawaki, Hiroyuki Miyoshi, Takeshi Imamura, 2008, Cell.

Selective excitation between two-photon and three-photon fluorescence with engineered cost functions.

Katsumi Midorikawa, Fumihiko Kannari, Takasumi Tanabe, 2004, Optics express.

Structural characterization of a thiazoline-containing chromophore in an orange fluorescent protein, monomeric Kusabira Orange.

Atsushi Miyawaki, Hideaki Mizuno, Satoshi Karasawa, 2008, Biochemistry.

A discrete neuronal circuit induces a hibernation-like state in rodents

Takeshi Sakurai, Atsushi Miyawaki, Masashi Yanagisawa, 2020, Nature.

Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer.

Takeharu Nagai, Atsushi Miyawaki, Hideaki Mizuno, 2004, The Biochemical journal.

Shift anticipated in DNA microarray market

Takeharu Nagai, Atsushi Miyawaki, Katsuhiko Mikoshiba, 2002, Nature Biotechnology.

Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa.

Peter Dedecker, Atsushi Miyawaki, Hideaki Mizuno, 2005, Proceedings of the National Academy of Sciences of the United States of America.

Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein Kaede.

Atsushi Miyawaki, Hideaki Mizuno, Mitsuhiko Ikura, 2007, Journal of molecular biology.

Implementation of spatial overlap modulation nonlinear optical microscopy using an electro-optic deflector.

Katsumi Midorikawa, Atsushi Miyawaki, Keisuke Isobe, 2013, Biomedical optics express.

Proteins on the move: insights gained from fluorescent protein technologies

Atsushi Miyawaki, A. Miyawaki, 2011, Nature Reviews Molecular Cell Biology.

GFP-like proteins stably accumulate in lysosomes.

Atsushi Miyawaki, A. Miyawaki, A. Yamamoto, 2008, Cell structure and function.

Controllable alignment of elongated microorganisms in 3D microspace using electrofluidic devices manufactured by hybrid femtosecond laser microfabrication

Koji Sugioka, Katsumi Midorikawa, Yasutaka Hanada, 2017, Microsystems & Nanoengineering.

Structural Characterization of a Blue Chromoprotein and Its Yellow Mutant from the Sea Anemone Cnidopus Japonicus*

Atsushi Miyawaki, Hideaki Mizuno, Mitsuhiko Ikura, 2006, Journal of Biological Chemistry.