Jürgen Hubbuch

发表

Editorial: High‐throughput process development

Jürgen Hubbuch, J. Hubbuch, 2012 .

Implementation of an analytical microfluidic device for the quantification of protein concentrations in high‐throughput format

Jürgen Hubbuch, Carsten P. Radtke, Marie-Therese Schermeyer, 2016 .

Straightforward method for calibration of mechanistic cation exchange chromatography models for industrial applications.

Gang Wang, Jürgen Hubbuch, David Saleh, 2020, Biotechnology progress.

Examination of a genetic algorithm for the application in high-throughput downstream process development.

Jürgen Hubbuch, Katrin Treier, Annette Berg, 2012, Biotechnology journal.

High-throughput screening-based selection and scale-up of aqueous two-phase systems for pDNA purification.

Jürgen Hubbuch, Florian Dismer, Matthias Wiendahl, 2012, Journal of separation science.

MIE Summarizing Report

Jürgen Hubbuch, Wolfgang Wiechert, 2015 .

High-throughput downstream process development for cell-based products using aqueous two-phase systems (ATPS) - A case study.

Jürgen Hubbuch, Sarah Zimmermann, Christian Scheeder, 2017, Biotechnology journal.

Automated image processing as an analytical tool in cell cryopreservation for bioprocess development

Jürgen Hubbuch, Sarah Gretzinger, Stefanie Limbrunner, 2019, Bioprocess and Biosystems Engineering.

Application of genetic algorithms and response surface analysis for the optimization of batch chromatographic systems

Jürgen Hubbuch, Katrin Treier, Philip Lester, 2012 .

Image analysis as PAT-Tool for use in extrusion-based bioprinting

Jürgen Hubbuch, Sarah Gretzinger, Svenja Strauß, 2021, Bioprinting.

High throughput screening techniques in downstream processing: Preparation, characterization and optimization of aqueous two-phase systems

Jürgen Hubbuch, Matthias Bensch, Björn Selbach, 2007 .

Spectral deconvolution of chromatograms without offline analytics

Jürgen Hubbuch, Florian Dismer, Matthias Rüdt, 2015 .

Rational and systematic protein purification process development: the next generation.

Jürgen Hubbuch, Marcel Ottens, Beckley K Nfor, 2009, Trends in biotechnology.

Printed Biological Systems: Using Nature's Toolbox for Novel Biotechnical Applications

Wolfgang Wiechert, Jürgen Hubbuch, I. Perner-Nochta, 2018 .

Microfluidics on liquid handling stations (μF-on-LHS): an industry compatible chip interface between microfluidics and automated liquid handling stations.

Jürgen Hubbuch, Ansgar Waldbaur, Bastian E Rapp, 2013, Lab on a chip.

PAT concepts for chromatography: Real-time monitoring, real-time control, and cause of error diagnostics

Jürgen Hubbuch, Tobias Hahn, Nina Brestrich, 2015 .

Molecular Interaction Engineering

Wolfgang Wiechert, Jürgen Hubbuch, I. Perner-Nochta, 2014 .

High Throughput Screening of Chromatographic Phases for Rapid Process Development

Jürgen Hubbuch, Matthias Bensch, E. von Lieres, 2005 .

Microfluidics on liquid handling stations (μF-on-LHS): a new industry-compatible microfluidic platform

Jürgen Hubbuch, Carsten P. Radtke, Bastian E. Rapp, 2014, Photonics West - Micro and Nano Fabricated Electromechanical and Optical Components.

3D bioprinting – Flow cytometry as analytical strategy for 3D cell structures

Jürgen Hubbuch, Andrew Gleadall, Sarah Gretzinger, 2018, Bioprinting.

A critical review of recent trends, and a future perspective of optical spectroscopy as PAT in biopharmaceutical downstream processing

Jürgen Hubbuch, Matthias Rüdt, Laura Rolinger, 2020, Analytical and Bioanalytical Chemistry.

Advances in inline quantification of co‐eluting proteins in chromatography: Process‐data‐based model calibration and application towards real‐life separation issues

Jürgen Hubbuch, Axel Kraft, Joseph Bertolini, 2015, Biotechnology and bioengineering.

Model-based integrated optimization and evaluation of a multi-step ion exchange chromatography

Jürgen Hubbuch, Tobias Hahn, Anna Osberghaus, 2014 .

Feasibility of using continuous chromatography in downstream processing: Comparison of costs and product quality for a hybrid process vs. a conventional batch process.

Jürgen Hubbuch, Ozan Ötes, Hendrik Flato, 2017, Journal of biotechnology.

A label‐free methodology for selective protein quantification by means of absorption measurements

Jürgen Hubbuch, Sigrid K Hansen, Arne Staby, 2011, Biotechnology and bioengineering.

Molecular dynamics simulations on aqueous two-phase systems - Single PEG-molecules in solution

Jürgen Hubbuch, Stefan A Oelmeier, Florian Dismer, 2012, BMC biophysics.

High‐throughput methods for miniaturization and automation of monoclonal antibody purification processes

Jürgen Hubbuch, Katrin Treier, Patrick Diederich, 2012, Biotechnology progress.

Modeling of hydrophobic interaction chromatography for the separation of antibody-drug conjugates and its application towards quality by design.

Jürgen Hubbuch, Sebastian Andris, J. Hubbuch, 2020, Journal of biotechnology.

Next generation vaccines and vectors: Designing downstream processes for recombinant protein‐based virus‐like particles

Jürgen Hubbuch, Christopher Ladd Effio, J. Hubbuch, 2015, Biotechnology journal.

Unavoidable variance trends in data obtained by experiments on robotic platforms – how to consider such effects in model parameter estimation

Jürgen Hubbuch, E. von Lieres, A. Siudak, 2009 .

Model-integrated process development demonstrated on the optimization of a robotic cation exchange step

Jürgen Hubbuch, M. Haindl, Anna Osberghaus, 2012 .

High‐throughput micro‐scale cultivations and chromatography modeling: Powerful tools for integrated process development

Jürgen Hubbuch, Tobias Hahn, Pascal Baumann, 2015, Biotechnology and bioengineering.

A multisensor approach for improved protein A load phase monitoring by conductivity‐based background subtraction of UV spectra

Jürgen Hubbuch, Matthias Rüdt, Laura Rolinger, 2020, Biotechnology and bioengineering.

Implementation of entry-level bioprinters for biotechnological applications

Jürgen Hubbuch, Josefine Morgenstern Carsten P Radtke, 2018 .

Detection, Quantification, and Propagation of Uncertainty in High‐Throughput Experimentation by Monte Carlo Methods

Jürgen Hubbuch, M. Haindl, Anna Osberghaus, 2012 .

Downstream process development strategies for effective bioprocesses: Trends, progress, and combinatorial approaches

Jürgen Hubbuch, Pascal Baumann, J. Hubbuch, 2017, Engineering in life sciences.

Time-dependent multi-light source image classification combined with automated multidimensional protein phase diagram construction for protein phase behavior analysis.

Jürgen Hubbuch, Marieke E Klijn, 2019, Journal of pharmaceutical sciences.

Adjoint-based estimation and optimization for column liquid chromatography models

Jürgen Hubbuch, Vincent Heuveline, Tobias Hahn, 2014, Comput. Chem. Eng..

High Throughput Screening for the Design and Optimization of Chromatographic Processes: Assessment of Model Parameter Determination from High Throughput Compatible Data

Jürgen Hubbuch, E. von Lieres, A. Susanto, 2008 .

Computational study of elements of stability of a four-helix bundle protein biosurfactant

Jürgen Hubbuch, Anton P. J. Middelberg, Natalie K. Connors, 2014, Journal of Computer-Aided Molecular Design.

High throughput screening setup of a scale‐down device for membrane chromatography‐aggregate removal of monoclonal antibodies

Jürgen Hubbuch, Volkmar Thom, Dominik Stein, 2020, Biotechnology progress.

Impact of additives on the formation of protein aggregates and viscosity in concentrated protein solutions.

Jürgen Hubbuch, J. Hubbuch, Katharina Christin Bauer, 2017, International journal of pharmaceutics.

Real‐time monitoring and control of the load phase of a protein A capture step

Jürgen Hubbuch, Matthias Rüdt, Nina Brestrich, 2016, Biotechnology and bioengineering.

Concentration-dependent changes in apparent diffusion coefficients as indicator for colloidal stability of protein solutions.

Jürgen Hubbuch, Marie-Therese Schermeyer, J. Hubbuch, 2016, International journal of pharmaceutics.

Analytical characterization of complex, biotechnological feedstocks by pH gradient ion exchange chromatography for purification process development.

Jürgen Hubbuch, Marcel Ottens, Beckley K Nfor, 2013, Journal of chromatography. A.

Integrated bioprocesses.

Jürgen Hubbuch, Karl Schügerl, K. Schügerl, 2005, Current opinion in microbiology.

On-Demand Production of Flow-Reactor Cartridges by 3D Printing of Thermostable Enzymes.

Jürgen Hubbuch, Christof M Niemeyer, Kersten S Rabe, 2018, Angewandte Chemie.

Determination of protein phase diagrams by microbatch experiments: exploring the influence of precipitants and pH.

Jürgen Hubbuch, Stefan A Oelmeier, J. Hubbuch, 2015, International journal of pharmaceutics.

A novel approach to characterize the binding orientation of lysozyme on ion-exchange resins.

Jürgen Hubbuch, Florian Dismer, J. Hubbuch, 2007, Journal of chromatography. A.

A novel method to evaluate protein solubility using a high throughput screening approach

Jürgen Hubbuch, Stephan Scholl, Matthias Wiendahl, 2009 .

Modeling and simulation of anion-exchange membrane chromatography for purification of Sf9 insect cell-derived virus-like particles.

Jürgen Hubbuch, Stefan A Oelmeier, Christopher Ladd Effio, 2016, Journal of chromatography. A.

Fourier-transform infrared spectroscopy as a process analytical technology for near real time in-line estimation of the degree of PEGylation in chromatography.

Jürgen Hubbuch, Matthias Rüdt, Adrian Sanden, 2019, Journal of chromatography. A.

Multi-attribute PAT for UF/DF of Proteins—Monitoring Concentration, particle sizes, and Buffer Exchange

Jürgen Hubbuch, Matthias Rüdt, Martin Heitmann, 2020, Analytical and Bioanalytical Chemistry.

High throughput screening based selection of phases for aqueous two-phase system-centrifugal partitioning chromatography of monoclonal antibodies.

Jürgen Hubbuch, Stefan A Oelmeier, J. Hubbuch, 2012, Journal of chromatography. A.

Selective protein quantification for preparative chromatography using variable pathlength UV/Vis spectroscopy and partial least squares regression

Jürgen Hubbuch, Matthias Rüdt, Nina Brestrich, 2018 .

Simulating and Optimizing Preparative Protein Chromatography with ChromX

Jürgen Hubbuch, Vincent Heuveline, Thiemo Huuk, 2015 .

Effects of ionic strength and mobile phase pH on the binding orientation of lysozyme on different ion-exchange adsorbents.

Jürgen Hubbuch, Martin Petzold, Florian Dismer, 2008, Journal of chromatography. A.

UV absorption‐based inverse modeling of protein chromatography

Jürgen Hubbuch, Vincent Heuveline, Pascal Baumann, 2016 .

Molecular dynamics simulations of aqueous two-phase systems: Understanding phase formation and protein partitioning

Jürgen Hubbuch, Florian Dismer, Stefan A. Oelmeier, 2013 .

High-throughput downstream process development for cell-based products using aqueous two-phase systems.

Jürgen Hubbuch, Stefan A. Oelmeier, Sarah Zimmermann, 2016, Journal of chromatography. A.

Manipulation of lysozyme phase behavior by additives as function of conformational stability.

Jürgen Hubbuch, J. Hubbuch, Josefine Morgenstern, 2015, International journal of pharmaceutics.

A versatile noninvasive method for adsorber quantification in batch and column chromatography based on the ionic capacity

Jürgen Hubbuch, Till Briskot, J. Hubbuch, 2016, Biotechnology progress.

Alternative separation steps for monoclonal antibody purification: combination of centrifugal partitioning chromatography and precipitation.

Jürgen Hubbuch, Stefan A Oelmeier, J. Hubbuch, 2013, Journal of chromatography. A.

Calibration‐free inverse modeling of ion‐exchange chromatography in industrial antibody purification

Jürgen Hubbuch, Vincent Heuveline, Anna Osberghaus, 2016 .

Modeling of complex antibody elution behavior under high protein load densities in ion exchange chromatography using an asymmetric activity coefficient.

Jürgen Hubbuch, Stefan Hepbildikler, J. Hubbuch, 2017, Biotechnology journal.

Root cause investigation of deviations in protein chromatography based on mechanistic models and artificial neural networks.

Jürgen Hubbuch, Pascal Baumann, Till Briskot, 2017, Journal of chromatography. A.

Characterization of highly concentrated antibody solution - A toolbox for the description of protein long-term solution stability

Jürgen Hubbuch, Marie-Therese Schermeyer, M. Eppink, 2017, mAbs.

Analysis of phase behavior and morphology during freeze‐thaw applications of lysozyme

Jürgen Hubbuch, J. Hubbuch, Anna Katharina Wöll, 2019, International journal of pharmaceutics.

An integrated precipitation and ion-exchange chromatography process for antibody manufacturing: Process development strategy and continuous chromatography exploration.

Gang Wang, Jürgen Hubbuch, G. Wang, 2018, Journal of chromatography. A.

Machine-assisted cultivation and analysis of biofilms

Jürgen Hubbuch, Carsten P. Radtke, Michael Wagner, 2017 .

High Throughput Screening for the Design and Optimization of Chromatographic Processes: Automated Optimization of Chromatographic Phase Systems

Jürgen Hubbuch, Katrin Treier, E. von Lieres, 2009 .

Process monitoring of virus‐like particle reassembly by diafiltration with UV/Vis spectroscopy and light scattering

Jürgen Hubbuch, Matthias Rüdt, J. Hubbuch, 2019, Biotechnology and bioengineering.

Prediction uncertainty assessment of chromatography models using Bayesian inference.

Jürgen Hubbuch, Till Briskot, Stefan Hepbildikler, 2019, Journal of chromatography. A.

A high-throughput 2D-analytical technique to obtain single protein parameters from complex cell lysates for in silico process development of ion exchange chromatography.

Jürgen Hubbuch, D. Elsässer, J. Hubbuch, 2013, Journal of chromatography. A.

High‐throughput screening of packed‐bed chromatography coupled with SELDI‐TOF MS analysis: monoclonal antibodies versus host cell protein

Jürgen Hubbuch, J. Hubbuch, Ralf Bogumil, 2007, Biotechnology and bioengineering.

Application of an aqueous two‐phase systems high‐throughput screening method to evaluate mAb HCP separation

Jürgen Hubbuch, Stefan A Oelmeier, Florian Dismer, 2011, Biotechnology and bioengineering.

A novel two-zone protein uptake model for affinity chromatography and its application to the description of elution band profiles of proteins fused to a family 9 cellulose binding module affinity tag.

Jürgen Hubbuch, Florian Dismer, Mojgan Kavoosi, 2007, Journal of chromatography. A.

Estimation of adsorption isotherm and mass transfer parameters in protein chromatography using artificial neural networks.

Jürgen Hubbuch, Pascal Baumann, Till Briskot, 2017, Journal of chromatography. A.

High Throughput Screening for the Design and Optimization of Chromatographic Processes - Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies

Jürgen Hubbuch, Jens Nielsen, Matthias Wiendahl, 2008 .

Accurate retention time determination of co‐eluting proteins in analytical chromatography by means of spectral data

Jürgen Hubbuch, Florian Dismer, Sigrid Hansen, 2013, Biotechnology and bioengineering.