V. Rubio

发表

The crystal structure of Pyrococcus furiosus UMP kinase provides insight into catalysis and regulation in microbial pyrimidine nucleotide biosynthesis.

C. Marco-Marín, F. Gil-Ortiz, V. Rubio, 2005, Journal of molecular biology.

Arabidopsis CAND1, an Unmodified CUL1-Interacting Protein, Is Involved in Multiple Developmental Pathways Controlled by Ubiquitin/Proteasome-Mediated Protein Degradation

V. Rubio, X. Deng, Y. Xiong, 2004, The Plant Cell Online.

Plant hormones and nutrient signaling

Javier Paz-Ares, V. Rubio, Regla Bustos, 2009, Plant Molecular Biology.

A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae.

V. Rubio, A. C. Martin, R. Solano, 2001, Genes & development.

Mutations at CRE1 impair cytokinin-induced repression of phosphate starvation responses in Arabidopsis.

V. Rubio, J. Franco-Zorrilla, R. Solano, 2002, The Plant journal : for cell and molecular biology.

DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis

Kris Gevaert, Chris Bowler, Auguste Genovesio, 2018, bioRxiv.

V. Rubio, H. Mandel, J. Häberle, 2019, Journal of inherited metabolic disease.

acetyl-L-glutamate Synthase Deficiency: Mutational Spectrum, Impact of Clinical Mutations on Enzyme

C. Marco-Marín, V. Rubio, E. Sancho-Vaello, 2018 .

Disorders of the Urea Cycle and Related Enzymes

V. Rubio, J. Häberle, 2016 .

An alternative tandem affinity purification strategy applied to Arabidopsis protein complex isolation.

V. Rubio, X. Deng, S. Dinesh-Kumar, 2005, The Plant journal : for cell and molecular biology.

Structure of acetylglutamate kinase, a key enzyme for arginine biosynthesis and a prototype for the amino acid kinase enzyme family, during catalysis.

F. Gil-Ortiz, V. Rubio, A. Marina, 2002, Structure.

Structural Insight into Partner Specificity and Phosphoryl Transfer in Two-Component Signal Transduction

Alberto Marina, V. Rubio, P. Casino, 2009, Cell.

Gibberellins modulate light signaling pathways to prevent Arabidopsis seedling de-etiolation in darkness.

V. Rubio, X. Deng, D. Alabadí, 2007, The Plant journal : for cell and molecular biology.

COP1 destabilizes DELLA proteins in Arabidopsis

Matias D. Zurbriggen, V. Rubio, S. Kay, 2020, Proceedings of the National Academy of Sciences.

Site-directed mutagenesis of Escherichia coli acetylglutamate kinase and aspartokinase III probes the catalytic and substrate-binding mechanisms of these amino acid kinase family enzymes and allows three-dimensional modelling of aspartokinase.

C. Marco-Marín, V. Rubio, S. Ramón-Maiques, 2003, Journal of molecular biology.

The Spanish Society of Biochemistry and Molecular Biology, the development of biochemistry in Spain, and IUBMB

V. Rubio, 2008, IUBMB life.

Δ1‐Pyrroline‐5‐carboxylate synthetase deficiency: An emergent multifaceted urea cycle‐related disorder

C. Marco-Marín, V. Rubio, J. Llácer, 2020, Journal of inherited metabolic disease.

Domain structure of the large subunit of Escherichia coli carbamoyl phosphate synthetase. Location of the binding site for the allosteric inhibitor UMP in the COOH-terminal domain.

V. Rubio, C. J. Lusty, J. Cervera, 1991, Biochemistry.

Mechanism of carbamoyl-phosphate synthetase. Properties of the two binding sites for ATP.

V. Rubio, S. Grisolía, H. Britton, 1979, European journal of biochemistry.

Mechanism of carbamoyl-phosphate synthetase. Binding of ATP by the rat-liver mitochondrial enzyme.

V. Rubio, S. Grisolía, H. Britton, 1979, European journal of biochemistry.

Mitochondrial carbamoyl phosphate synthetase activity in the absence of N-acetyl-L-glutamate. Mechanism of activation by this cofactor.

V. Rubio, S. Grisolía, H. Britton, 1983, European journal of biochemistry.

Physical location of the site for N-acetyl-L-glutamate, the allosteric activator of carbamoyl phosphate synthetase, in the 20-kilodalton COOH-terminal domain.

V. Rubio, L. Rodríguez-Aparicio, A. Guadalajara, 1989, Biochemistry.

The influence of effectors and subunit interactions on Escherichia coli carbamoyl-phosphate synthetase studied by differential scanning calorimetry.

V. Rubio, C. J. Lusty, F. Conejero-Lara, 1993, The Journal of biological chemistry.

Location of the binding site for the allosteric activator IMP in the COOH-terminal domain of Escherichia coli carbamyl phosphates synthetase.

V. Rubio, C. J. Lusty, J. Bueso, 1994, Biochemical and biophysical research communications.

The carbamoyl-phosphate synthase family and carbamate kinase: structure-function studies.

V. Rubio, J. Cervera, 1995, Biochemical Society transactions.

Identification of Molecular Integrators Shows that Nitrogen Actively Controls the Phosphate Starvation Response in Plants

G. Krouk, V. Rubio, H. Rouached, 2019, Plant Cell.

Proteomics identifies ubiquitin-proteasome targets and new roles for chromatin-remodeling in the Arabidopsis response to phosphate starvation.

V. Rubio, Regla Bustos, Mónica Rojas-Triana, 2013, Journal of proteomics.

Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis thaliana by Promoting Nuclear Proteasome-Mediated HY5 Degradation.

V. Rubio, Elisa Iniesto, J. Salinas, 2017, Molecular plant.

Influence of anions on the activation of carbamoyl phosphate synthetase (ammonia) by acetylglutamate: implications for the activation of the enzyme in the mitochondria.

V. Rubio, A. García-España, E. Alonso, 1991, Archives of biochemistry and biophysics.

Carbamoyl phosphate synthetase I of human liver. Purification, some properties and immunological cross-reactivity with the rat liver enzyme.

V. Rubio, S. Grisolía, G. Ramponi, 1981, Biochimica et biophysica acta.

Mechanism of mitochondrial carbamoyl-phosphate synthetase: synthesis and properties of active CO2, precursor of carbamoyl phosphate.

V. Rubio, S. Grisolía, 1977, Biochemistry.

KISS ME DEADLY F-box proteins modulate cytokinin responses by targeting the transcription factor TCP14 for degradation.

V. Rubio, J. Paz-Ares, D. Weiss, 2021, Plant physiology.

Arabidopsis CRL4 Complexes: Surveying Chromatin States and Gene Expression

V. Rubio, S. Fonseca, Sandra Fonseca, 2019, Front. Plant Sci..

Improved cross-linked enzyme aggregates for the production of desacetyl beta-lactam antibiotics intermediates.

F. Gil-Ortiz, V. Rubio, F. García-Carmona, 2010, Bioresource technology.

COP1 promotes seed germination by destabilizing RGA-LIKE2 (RGL2) in Arabidopsis

V. Rubio, S. Fonseca, Byoung-Doo Lee, 2021, bioRxiv.

Multifaceted Activities of the Plant SAGA Complex.

V. Rubio, F. Barneche, K. Grasser, 2020, Biochimica et biophysica acta. Gene regulatory mechanisms.

The Arabidopsis Iron-Sulfur Protein GRXS17 is a Target of the Ubiquitin E3 Ligases RGLG3 and RGLG4.

V. Rubio, Andrés Ritter, Jelle Van Leene, 2016, Plant & cell physiology.

Novel signals in the regulation of Pi starvation responses in plants: facts and promises.

V. Rubio, Mónica Rojas-Triana, J. Paz-Ares, 2017, Current opinion in plant biology.

Understanding pyrroline-5-carboxylate synthetase deficiency: clinical, molecular, functional, and expression studies, structure-based analysis, and novel therapy with arginine

M. Baumgartner, C. Marco-Marín, V. Rubio, 2012, Journal of Inherited Metabolic Disease.

PipY, a Member of the Conserved COG0325 Family of PLP-Binding Proteins, Expands the Cyanobacterial Nitrogen Regulatory Network

V. Rubio, A. Contreras, J. Espinosa, 2017, Front. Microbiol..

Structural and Functional Insights into Endoglin Ligand Recognition and Binding

V. Rubio, F. Blanco, A. Round, 2012, PloS one.

The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery

V. Rubio, P. Robles, J. Micol, 2022, bioRxiv.

Palanca C.,Rubio V. (2016). Structure of AmtR, the global nitrogen regulator of Corynebacterium glutamicum, in free and DNA-bound forms. FEBS J 283(6): 1039-59 Author's last version

V. Rubio, C. Palanca, 2015 .

N-acetyl-l-glutamate in brain: Assay, levels, and regional and subcellular distribution

V. Rubio, M. García-Pérez, J. Bueso, 1991, Neurochemical Research.

Molecular defects in human carbamoy phosphate synthetase I: mutational spectrum, diagnostic and protein structure considerations

O. Lichtarge, V. Rubio, A. Willis, 2011, Human mutation.

Functional and structural characterization of PII‐like protein CutA does not support involvement in heavy metal tolerance and hints at a small‐molecule carrying/signaling role

C. Marco-Marín, V. Rubio, K. Forchhammer, 2020, The FEBS journal.

Localization of the site for the nucleotide effectors of Escherichia coli carbamoyl phosphate synthetase using site‐directed mutagenesis

V. Rubio, V. Fresquet, J. Cervera, 1999, FEBS letters.

Carbamate kinase from Enterococcus faecalis and Enterococcus faecium--cloning of the genes, studies on the enzyme expressed in Escherichia coli, and sequence similarity with N-acetyl-L-glutamate kinase.

V. Rubio, A. Marina, V. Fresquet, 1998, European journal of biochemistry.

Inhibition of lactic dehydrogenase and of pyruvate kinase by low concentrations of quercetin.

V. Rubio, S. Grisolía, B. Feijóo, 1975, Physiological chemistry and physics.

Identification of Mitofusin 1 and Complement Component 1q Subcomponent Binding Protein as Mitochondrial Targets in Systemic Lupus Erythematosus

F. Boisvert, V. Rubio, J. Gagné, 2022, Arthritis & rheumatology.

Light and the E3 ubiquitin ligase COP1/SPA control the protein stability of the MYB transcription factors PAP1 and PAP2 involved in anthocyanin accumulation in Arabidopsis.

V. Rubio, M. Hülskamp, J. Uhrig, 2013, The Plant journal : for cell and molecular biology.

Site‐directed mutagenesis studies of acetylglutamate synthase delineate the site for the arginine inhibitor

V. Rubio, E. Sancho-Vaello, M. L. Fernández-Murga, 2008, FEBS Letters.

Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures.

V. Rubio, Elisa Iniesto, M. García-León, 2018, Methods in molecular biology.

Plant proteostasis: a proven and promising target for crop improvement.

V. Rubio, F. Theodoulou, M. Trujillo, 2022, Essays in biochemistry.

ALDH18A1 gene mutations cause dominant spastic paraplegia SPG9: loss of function effect and plausibility of a dominant negative mechanism.

R. Ravazzolo, C. Marco-Marín, V. Rubio, 2016, Brain : a journal of neurology.

Hyperammonemias and related disorders

V. Rubio, J. Häberle, 2014 .

Mechanism of arginine regulation of acetylglutamate synthase, the first enzyme of arginine synthesis

V. Rubio, E. Sancho-Vaello, M. L. Fernández-Murga, 2009, FEBS letters.

The Gene Cluster for Agmatine Catabolism of Enterococcus faecalis: Study of Recombinant Putrescine Transcarbamylase and Agmatine Deiminase and a Snapshot of Agmatine Deiminase Catalyzing Its Reaction

V. Rubio, J. Llácer, S. Tavárez, 2006, Journal of bacteriology.

Demonstration of the spf-ash mutation in Spanish patients with ornithine transcarbamylase deficiency of moderate severity

V. Rubio, M. García-Pérez, P. Sanjurjo, 1995, Human Genetics.

Carbamate Synthases and Kinases

V. Rubio, I. Climent, H. Britton, 1990 .

Transient hyperammonemias in infants with and without organic acidemia.

V. Rubio, S. Grisolía, W. Nyhan, 1982, Advances in experimental medicine and biology.

N-Acetyl-L-glutamate kinase from Escherichia coli: cloning of the gene, purification and crystallization of the recombinant enzyme and preliminary X-ray analysis of the free and ligand-bound forms.

V. Rubio, A. Marina, S. Ramón-Maiques, 1999, Acta crystallographica. Section D, Biological crystallography.

Crystallization, characterization, and preliminary crystallographic studies of mitochondrial carbamoyl phosphate synthetase I of Rana catesbeiana

V. Rubio, J. Bravo, A. Marina, 1995, Proteins.

Affinity cleavage of carbamoyl-phosphate synthetase I localizes regions of the enzyme interacting with the molecule of ATP that phosphorylates carbamate.

V. Rubio, E. Alonso, 1995, European journal of biochemistry.

Levels of carbamoyl phosphate synthetase I in livers of young and old rats assessed by activity and immunoassays and by electron microscopic immunogold procedures.

V. Rubio, S. Grisolía, E. Knecht, 1990, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

Changes in urea cycle-related metabolites in the mouse after combined administration of valproic acid and an amino acid load.

V. Rubio, J. Girbés, A. García-España, 1989, Archives of biochemistry and biophysics.

The Conserved Family of the Pyridoxal Phosphate-Binding Protein (PLPBP) and Its Cyanobacterial Paradigm PipY

V. Rubio, A. Contreras, Antonio Llop, 2022, Life.

P5CS expression study in a new family with ALDH18A1‐associated hereditary spastic paraplegia SPG9

C. Marco-Marín, V. Rubio, F. Faravelli, 2019, Annals of clinical and translational neurology.

Binding of N-acetyl-L-glutamate to rat liver carbamoyl phosphate synthetase (ammonia).

V. Rubio, E. Alonso, 1983, European journal of biochemistry.

Synthesis of carbamoyl phosphate by carbamoyl phosphate synthetase I in the absence of acetylglutamate. Activation of the enzyme by cryoprotectants.

V. Rubio, S. Grisolía, H. Britton, 1981, Biochemical and biophysical research communications.

Rheostatic control of ABA signaling through HOS15-mediated OST1 degradation.

Jae Kyoung Kim, V. Rubio, Woe-Yeon Kim, 2019, Molecular plant.

LZF 1 / SALT TOLERANCE HOMOLOG 3 , an Arabidopsis BBox Protein Involved in Light-Dependent Development and Gene Expression , Undergoes COP 1-Mediated Ubiquitination

V. Rubio, S. Datta, H. Johansson, 2008 .

CONSTITUTIVELY PHOTOMORPHOGENIC1 promotes seed 2 germination by destabilizing RGA-LIKE2 in Arabidopsis 3

V. Rubio, S. Fonseca, Byoung-Doo Lee, 2022 .

Inactivation of mitochondrial carbamoyl phosphate synthetase induced by ascorbate, oxygen, and Fe3+ in the presence of acetylglutamate: protection by ATP and HCO3- and lack of inactivation of ornithine transcarbamylase.

V. Rubio, E. Alonso, 1987, Archives of biochemistry and biophysics.

Determination of N-acetyl-L-glutamate using high-performance liquid chromatography.

V. Rubio, E. Alonso, 1985, Analytical biochemistry.

A Central Regulatory System Largely Controls Transcriptional Activation and Repression Responses to Phosphate Starvation in Arabidopsis

Javier Paz-Ares, V. Rubio, R. Solano, 2010, PLoS genetics.

Carbamoyl phosphate synthetase, ornithine transcarbamylase, and aspartate transcarbamylase activities in the pea ovary : changes with senescence of the unpollinated ovary or with fruit set induced by gibberellic Acid.

V. Rubio, J. Carbonell, A. García-España, 1989, Plant physiology.

Phy Tunes: Phosphorylation Status and Phytochrome-Mediated Signaling

V. Rubio, X. Deng, 2005, Cell.

Structure-function studies in carbamoyl phosphate synthetases.

V. Rubio, 1993, Biochemical Society transactions.

Orotic aciduria due to arginine deprivation: changes in the levels of carbamoyl phosphate and of other urea cycle intermediates in mouse liver.

V. Rubio, E. Alonso, 1989, The Journal of nutrition.

A type 5 acid phosphatase gene from Arabidopsis thaliana is induced by phosphate starvation and by some other types of phosphate mobilising/oxidative stress conditions.

V. Rubio, J. C. del Pozo, J. Paz-Ares, 1999, The Plant journal : for cell and molecular biology.

Autoradiographic evidence of increased incorporation of aspartate and of carbamoyl aspartate in fibroblasts from a Lesch-Nyhan patient.

V. Rubio, S. Grisolía, A. Martínez-Ramón, 1979, Biochemical and biophysical research communications.

V. Rubio, N. Boddaert, J. Häberle, 2012, Orphanet Journal of Rare Diseases.

The PII-NAGK-PipX-NtcA Regulatory Axis of Cyanobacteria: A Tale of Changing Partners, Allosteric Effectors and Non-covalent Interactions

C. Marco-Marín, V. Rubio, A. Contreras, 2018, Front. Mol. Biosci..

Expanding the Cyanobacterial Nitrogen Regulatory Network: The GntR-Like Regulator PlmA Interacts with the PII-PipX Complex

V. Rubio, P. Salinas, A. Contreras, 2016, Front. Microbiol..

Structural basis for the regulation of NtcA-dependent transcription by proteins PipX and PII

V. Rubio, K. Forchhammer, A. Contreras, 2010, Proceedings of the National Academy of Sciences.

The crystal structure of the cephalosporin deacetylating enzyme acetyl xylan esterase bound to paraoxon explains the low sensitivity of this serine hydrolase to organophosphate inactivation.

F. Gil-Ortiz, V. Rubio, F. García-Carmona, 2011, The Biochemical journal.

CUL3BPM E3 ubiquitin ligases regulate MYC2, MYC3, and MYC4 stability and JA responses

V. Rubio, J. Franco-Zorrilla, R. Solano, 2020, Proceedings of the National Academy of Sciences.

PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 Is a Plant-Specific SEC12-Related Protein That Enables the Endoplasmic Reticulum Exit of a High-Affinity Phosphate Transporter in Arabidopsis[W]

V. Rubio, R. Solano, J. Paz-Ares, 2005, The Plant Cell Online.

Arabidopsis COP1/SPA1 complex and FHY1/FHY3 associate with distinct phosphorylated forms of phytochrome A in balancing light signaling.

V. Rubio, Haiyang Wang, X. Deng, 2008, Molecular cell.

Arginine and nitrogen storage.

V. Rubio, I. Fita, J. Llácer, 2008, Current opinion in structural biology.

Understanding N‐Acetyl‐L‐Glutamate Synthase Deficiency: Mutational Spectrum, Impact of Clinical Mutations on Enzyme Functionality, and Structural Considerations

C. Marco-Marín, V. Rubio, E. Sancho-Vaello, 2016, Human mutation.

N‐carbamoylglutamate‐responsive carbamoyl phosphate synthetase 1 (CPS1) deficiency: A patient with a novel CPS1 mutation and an experimental study on the mutation's effects

V. Rubio, A. Hart, Nadine Gougeard, 2019, JIMD reports.

Human carbamoylphosphate synthetase I.

V. Rubio, S. Grisolía, 1981, Enzyme.

Treating urea cycle defects

V. Rubio, S. Grisolía, 1981, Nature.

Targeted Degradation of Abscisic Acid Receptors Is Mediated by the Ubiquitin Ligase Substrate Adaptor DDA1 in Arabidopsis[W]

V. Rubio, X. Deng, P. L. Rodriguez, 2014, Plant Cell.

Structure of human carbamoyl phosphate synthetase: deciphering the on/off switch of human ureagenesis

V. Rubio, I. Fita, L. M. Polo, 2015, Scientific Reports.

Recurrence of carbamoyl phosphate synthetase 1 (CPS1) deficiency in Turkish patients: characterization of a founder mutation by use of recombinant CPS1 from insect cells expression.

V. Rubio, A. Olgaç, J. Häberle, 2014, Molecular Genetics and Metabolism.

Structural insight on the control of urea synthesis: identification of the binding site for N-acetyl-L-glutamate, the essential allosteric activator of mitochondrial carbamoyl phosphate synthetase.

V. Rubio, S. Pekkala, J. Gallego, 2009, The Biochemical journal.

Activation of carbamoyl phosphate synthetase by cryoprotectants

V. Rubio, S. Grisolía, H. Britton, 2004, Molecular and Cellular Biochemistry.

The Study of Carbamoyl Phosphate Synthetase 1 Deficiency Sheds Light on the Mechanism for Switching On/Off the Urea Cycle.

V. Rubio, J. Häberle, J. Gallego, 2015, Journal of genetics and genomics = Yi chuan xue bao.

Mutant alleles associated with late-onset ornithine transcarbamylase deficiency in male patients have recurrently arisen and have been retained in some populations

J. A. Arranz, M. Tuchman, V. Rubio, 2010, Journal of Human Genetics.

A splicing mutation, a nonsense mutation (Y167X) and two missense mutations (I159T and A209V) in Spanish patients with ornithine transcarbamylase deficiency

V. Rubio, M. García-Pérez, P. Briones, 1995, Human Genetics.

Epigenetic switch from repressive to permissive chromatin in response to cold stress

V. Rubio, Woe-Yeon Kim, Sang Yeol Lee, 2018, Proceedings of the National Academy of Sciences.

The COP1-SPA1 interaction defines a critical step in phytochrome A-mediated regulation of HY5 activity.

V. Rubio, Haiyang Wang, X. Deng, 2003, Genes & development.

Crystal structures and functional studies clarify substrate selectivity and catalytic residues for the unique orphan enzyme N-acetyl-D-mannosamine dehydrogenase.

F. Gil-Ortiz, V. Rubio, F. García-Carmona, 2014, The Biochemical journal.

Participation of ornithine aminotransferase in the synthesis and catabolism of ornithine in mice. Studies using gabaculine and arginine deprivation.

V. Rubio, E. Alonso, 1989, The Biochemical journal.

A structure-reactivity study of the binding of acetylglutamate to carbamoyl phosphate synthetase I.

V. Rubio, I. Rozas, A. García-España, 1990, European journal of biochemistry.

Two crystal structures of Escherichia coli N-acetyl-L-glutamate kinase demonstrate the cycling between open and closed conformations.

Ignacio Fita, F. Gil-Ortiz, V. Rubio, 2010, Journal of molecular biology.

SPX1 is a phosphate-dependent inhibitor of PHOSPHATE STARVATION RESPONSE 1 in Arabidopsis

S. Masiero, V. Rubio, J. Franco-Zorrilla, 2014, Proceedings of the National Academy of Sciences.

Studies on cyanobacterial protein PipY shed light on structure, potential functions, and vitamin B6‐dependent epilepsy

V. Rubio, A. Contreras, Alicia Forcada-Nadal, 2017, FEBS letters.

Arginine Biosynthesis in Thermotoga maritima: Characterization of the Arginine-Sensitive N-Acetyl-l-Glutamate Kinase

F. Gil-Ortiz, V. Rubio, M. L. Fernández-Murga, 2004, Journal of bacteriology.

COP1 and ELF3 control circadian function and photoperiodic flowering by regulating GI stability.

V. Rubio, X. Deng, Ilha Lee, 2008, Molecular cell.

LZF1/SALT TOLERANCE HOMOLOG3, an Arabidopsis B-Box Protein Involved in Light-Dependent Development and Gene Expression, Undergoes COP1-Mediated Ubiquitination[W]

V. Rubio, M. Holm, M. L. Irigoyen, 2008, The Plant Cell Online.

Gene Structure, Organization, Expression, and Potential Regulatory Mechanisms of Arginine Catabolism in Enterococcus faecalis

V. Rubio, A. Marina, Belén Barcelona-Andrés, 2002, Journal of bacteriology.

Carbamate kinase: New structural machinery for making carbamoyl phosphate, the common precursor of pyrimidines and arginine

V. Rubio, J. Bravo, P. Alzari, 2008, Protein science : a publication of the Protein Society.

The mechanism of signal transduction by two-component systems.

V. Rubio, P. Casino, A. Marina, 2010, Current opinion in structural biology.

Purification of the COP9 signalosome from porcine spleen, human cell lines, and Arabidopsis thaliana plants.

V. Rubio, X. Deng, Xiping Wang, 2005, Methods in enzymology.

Structure of AmtR, the global nitrogen regulator of Corynebacterium glutamicum, in free and DNA‐bound forms

V. Rubio, C. Palanca, 2016, The FEBS journal.

The COP9 Signalosome: Structural and Biochemical Conservation and Its Roles in the Regulation of Plant Development

V. Rubio, X. Deng, 2007 .

The PipX Protein, When Not Bound to Its Targets, Has Its Signaling C-Terminal Helix in a Flexed Conformation.

V. Rubio, A. Pineda-Lucena, J. Neira, 2017, Biochemistry.

Ligand binding specificity of RutR, a member of the TetR family of transcription regulators in Escherichia coli

V. Rubio, D. Maes, D. Charlier, 2015, FEBS open bio.

Understanding carbamoyl phosphate synthetase deficiency: impact of clinical mutations on enzyme functionality.

Javier Cervera, C. Marco-Marín, V. Rubio, 2005, Journal of molecular biology.

Understanding carbamoyl phosphate synthetase (CPS1) deficiency by using the recombinantly purified human enzyme: effects of CPS1 mutations that concentrate in a central domain of unknown function.

V. Rubio, J. Häberle, Liyan Hu, 2014, Molecular genetics and metabolism.

Molecular Characterization of Carbamoyl‐Phosphate Synthetase (CPS1) Deficiency Using Human Recombinant CPS1 as a Key Tool

V. Rubio, I. Pérez-Arellano, M. Summar, 2013, Human mutation.

Understanding carbamoyl‐phosphate synthetase I (CPS1) deficiency by using expression studies and structure‐based analysis

V. Rubio, U. Finckh, S. Pekkala, 2010, Human mutation.

Site-directed mutagenesis of the regulatory domain of Escherichia coli carbamoyl phosphate synthetase identifies crucial residues for allosteric regulation and for transduction of the regulatory signals.

V. Rubio, S. Ramón-Maiques, V. Fresquet, 2000, Journal of molecular biology.

The dark side of clock-controlled flowering

V. Rubio, X. Deng, 2009, F1000 biology reports.

Insight on an Arginine Synthesis Metabolon from the Tetrameric Structure of Yeast Acetylglutamate Kinase

F. Gil-Ortiz, V. Rubio, M. Crabeel, 2012, PloS one.

First-time crystallization and preliminary X-ray crystallographic analysis of a bacterial-archaeal type UMP kinase, a key enzyme in microbial pyrimidine biosynthesis.

C. Marco-Marín, V. Rubio, Juan M Escamilla-Honrubia, 2005, Biochimica et biophysica acta.

Glutamate-5-kinase from Escherichia coli: gene cloning, overexpression, purification and crystallization of the recombinant enzyme and preliminary X-ray studies.

F. Gil-Ortiz, V. Rubio, I. Pérez-Arellano, 2004, Acta crystallographica. Section D, Biological crystallography.

Towards structural understanding of feedback control of arginine biosynthesis: cloning and expression of the gene for the arginine-inhibited N-acetyl-L-glutamate kinase from Pseudomonas aeruginosa, purification and crystallization of the recombinant enzyme and preliminary X-ray studies.

F. Gil-Ortiz, V. Rubio, M. L. Fernández-Murga, 2002, Acta crystallographica. Section D, Biological crystallography.

The Carbamoyl-phosphate Synthetase of Pyrococcus furiosus Is Enzymologically and Structurally a Carbamate Kinase*

V. Rubio, A. Marina, S. Ramón-Maiques, 1999, The Journal of Biological Chemistry.

Structural bases of feed-back control of arginine biosynthesis, revealed by the structures of two hexameric N-acetylglutamate kinases, from Thermotoga maritima and Pseudomonas aeruginosa.

Alexei Vagin, Ignacio Fita, F. Gil-Ortiz, 2006, Journal of molecular biology.

A novel two-domain architecture within the amino acid kinase enzyme family revealed by the crystal structure of Escherichia coli glutamate 5-kinase.

Ignacio Fita, Javier Cervera, C. Marco-Marín, 2007, Journal of molecular biology.

Mapping active site residues in glutamate‐5‐kinase. The substrate glutamate and the feed‐back inhibitor proline bind at overlapping sites

V. Rubio, I. Pérez-Arellano, J. Cervera, 2006, FEBS letters.

Roles of ubiquitination in the control of phosphate starvation responses in plants(f).

V. Rubio, S. Prat, Regla Bustos, 2013, Journal of integrative plant biology.

Basis of Arginine Sensitivity of Microbial N-Acetyl-l-Glutamate Kinases: Mutagenesis and Protein Engineering Study with the Pseudomonas aeruginosa and Escherichia coli Enzymes

V. Rubio, M. L. Fernández-Murga, Vicente Rubio, 2008, Journal of bacteriology.

Arabidopsis ALIX is required for the endosomal localization of the deubiquitinating enzyme AMSH3

V. Rubio, P. Braun, J. Paz-Ares, 2015, Proceedings of the National Academy of Sciences.

New Insight into the Transcarbamylase Family: The Structure of Putrescine Transcarbamylase, a Key Catalyst for Fermentative Utilization of Agmatine

F. Gil-Ortiz, V. Rubio, Á. Cantín, 2012, PloS one.

ESCRT-III-Associated Protein ALIX Mediates High-Affinity Phosphate Transporter Trafficking to Maintain Phosphate Homeostasis in Arabidopsis

V. Rubio, N. Geldner, M. L. Irigoyen, 2015, Plant Cell.

Influence of cytokinins on the expression of phosphate starvation responsive genes in Arabidopsis.

V. Rubio, R. Solano, J. C. del Pozo, 2000, The Plant journal : for cell and molecular biology.

N-Terminal Domain–Mediated Homodimerization Is Required for Photoreceptor Activity of Arabidopsis CRYPTOCHROME 1

V. Rubio, X. Deng, Y. Sang, 2005, The Plant Cell Online.

Arabidopsis COP10 forms a complex with DDB1 and DET1 in vivo and enhances the activity of ubiquitin conjugating enzymes.

Setsuko Komatsu, Jian Wang, Xing Wang Deng, 2004, Genes & development.

Photoaffinity labeling with the activator IMP and site-directed mutagenesis of histidine 995 of carbamoyl phosphate synthetase from Escherichia coli demonstrate that the binding site for IMP overlaps with that for the inhibitor UMP.

V. Rubio, C. J. Lusty, A. Marina, 1999, Biochemistry.

Functional dissection of N-acetylglutamate synthase ( ArgA ) of Pseudomonas 1 aeruginosa and restoration of its ancestral N-acetylglutamate kinase activity 2 3 Running title : Domain functions of acetylglutamate synthase 4 5

V. Rubio, E. Sancho-Vaello, M. L. Fernández-Murga, 2012 .

THE FUNCTION OF THE COP/DET/FUS PROTEINS INCONTROLLING PHOTOMORPHOGENESIS: A ROLE FOR REGULATED PROTEOLYSIS

V. Rubio, X. Deng, E. Strickland, 2006 .

DET1-mediated COP1 regulation avoids HY5 activity over second-site targets to tune plant photomorphogenesis

V. Rubio, S. Fonseca, F. Barneche, 2020, bioRxiv.

Structures of collagen IV globular domains: insight into associated pathologies, folding and network assembly

Alberto Marina, Jesús Rodríguez-Díaz, Javier Cervera, 2018, IUCrJ.

Interallelic complementation at the Arabidopsis CRE1 locus uncovers independent pathways for the proliferation of vascular initials and canonical cytokinin signalling.

V. Rubio, J. Paz-Ares, A. Leyva, 2004, The Plant journal : for cell and molecular biology.

Dissection of Escherichia coli glutamate 5‐kinase: Functional impact of the deletion of the PUA domain

V. Rubio, I. Pérez-Arellano, J. Cervera, 2005, FEBS letters.

Substrate binding and catalysis in carbamate kinase ascertained by crystallographic and site-directed mutagenesis studies: movements and significance of a unique globular subdomain of this key enzyme for fermentative ATP production in bacteria.

Alberto Marina, Ignacio Fita, F. Gil-Ortiz, 2010, Journal of molecular biology.

The site for the allosteric activator GTP of Escherichia coli UMP kinase

C. Marco-Marín, V. Rubio, 2009, FEBS letters.

The course of phosphorus in the reaction of N-acetyl-L-glutamate kinase, determined from the structures of crystalline complexes, including a complex with an AlF(4)(-) transition state mimic.

Ignacio Fita, F. Gil-Ortiz, V. Rubio, 2003, Journal of molecular biology.

ATPase activity of biotin carboxylase provides evidence for initial activation of HCO3- by ATP in the carboxylation of biotin.

V. Rubio, I. Climent, 1986, Archives of biochemistry and biophysics.

The structural role of SARS-CoV-2 genetic background in the emergence and success of spike mutations: The case of the spike A222V mutation

C. Marco-Marín, V. Rubio, J. Carazo, 2021, bioRxiv.

Oxidative inactivation of carbamoyl phosphate synthetase (ammonia). Mechanism and sites of oxidation, degradation of the oxidized enzyme, and inactivation by glycerol, EDTA, and thiol protecting agents.

V. Rubio, A. García-España, J. Cervera, 1992, The Journal of biological chemistry.

Carbamoyl-phosphate synthetase I. Kinetics of binding and dissociation of acetylglutamate and of activation and deactivation.

V. Rubio, H. Britton, 1988, European journal of biochemistry.

1 Arginine and Nitrogen Storage

V. Rubio, I. Fita, J. Llácer, 2008 .

The crystal structure of the complex of PII and acetylglutamate kinase reveals how PII controls the storage of nitrogen as arginine

Asunción Contreras, Ignacio Fita, C. Marco-Marín, 2007, Proceedings of the National Academy of Sciences.

Photoaffinity labeling with UMP of lysine 992 of carbamyl phosphate synthetase from Escherichia coli allows identification of the binding site for the pyrimidine inhibitor.

V. Rubio, C. J. Lusty, J. Cervera, 1996, Biochemistry.

Arabidopsis ALIX Regulates Stomatal Aperture and Turnover of Abscisic Acid Receptors

V. Rubio, P. L. Rodriguez, N. Leonhardt, 2019, Plant Cell.

H intragenic polymorphisms and haplotype analysis in the ornithine transcarbamylase (OTC) gene and their relevance for tracking the inheritance of OTC deficiency

V. Rubio, C. Climent, 2002, Human mutation.

Identification of Molecular Integrators Shows that Nitrogen Actively Controls the Phosphate Starvation Response in Plants.

G. Krouk, V. Rubio, H. Rouached, 2019, The Plant cell.

Mechanism of oligomerization of Escherichia coli carbamoyl phosphate synthetase and modulation by the allosteric effectors. A site‐directed mutagenesis study

V. Rubio, J. Cervera, P. Mora, 2002, FEBS letters.

Stomatal aperture and turnover of ABA receptors are regulated by Arabidopsis ALIX.

V. Rubio, P. L. Rodriguez, N. Leonhardt, 2019, The Plant cell.

Enzymatic HCO3− fixation: A common mechanism for all enzymes involved?

V. Rubio, 1986, Bioscience reports.

Functional Dissection of N-Acetylglutamate Synthase (ArgA) of Pseudomonas aeruginosa and Restoration of Its Ancestral N-Acetylglutamate Kinase Activity

V. Rubio, E. Sancho-Vaello, M. L. Fernández-Murga, 2012, Journal of bacteriology.

Biochemical and Imaging Analysis of ALIX Function in Endosomal Trafficking of Arabidopsis Protein Cargoes.

V. Rubio, M. García-León, 2020, Methods in molecular biology.

Limited proteolysis reveals low-affinity binding of N-acetyl-L-glutamate to rat-liver carbamoyl-phosphate synthetase (ammonia).

V. Rubio, S. Grisolía, A. Guadalajara, 1987, European journal of biochemistry.

Identification of seven novel missense mutations, two splice‐site mutations, two microdeletions and a polymorphic amino acid substitution in the gene for ornithine transcarbamylase (OTC) in patients with OTC deficiency

V. Rubio, C. Climent, 2002, Human mutation.

Carbamoyl phosphate synthesis: carbamate kinase from Pyrococcus furiosus.

N. Glansdorff, V. Rubio, C. Legrain, 2001, Methods in enzymology.

DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis

V. Rubio, A. Genovesio, Ikhlak Ahmed, 2018, bioRxiv.

Prominent role of lysosomes in the proteolysis of rat liver mitochondria at neutral pH

V. Rubio, S. Grisolía, 1977, FEBS letters.

The structure of a PII signaling protein from a halophilic archaeon reveals novel traits and high‐salt adaptations

V. Rubio, M. Bonete, J. Llácer, 2014, The FEBS journal.

Carbamate kinase can replace in vivo carbamoyl phosphate synthetase. Implications for the evolution of carbamoyl phosphate biosynthesis

V. Rubio, C. Alcántara, J. Cervera, 2000, FEBS letters.

COP1 and ELF3 control circadian function and photoperiodic flowering by regulating GI stability.

V. Rubio, X. Deng, Ilha Lee, 2008, Molecular cell.

The Study of Carbamoyl Phosphate Synthetase 1 Deficiency Sheds Light on the Mechanism for Switching On/Off the Urea Cycle.

V. Rubio, J. Häberle, J. Gallego, 2015, Journal of genetics and genomics = Yi chuan xue bao.

Structural insight on the control of urea synthesis: identification of the binding site for N-acetyl-L-glutamate, the essential allosteric activator of mitochondrial carbamoyl phosphate synthetase.

V. Rubio, S. Pekkala, J. Gallego, 2009, The Biochemical journal.

Mechanism of allosteric modulation of Escherichia coli carbamoyl phosphate synthetase probed by site‐directed mutagenesis of ornithine site residues

V. Rubio, V. Fresquet, J. Cervera, 2002, FEBS letters.

Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis thaliana by Promoting Nuclear Proteasome-Mediated HY5 Degradation.

V. Rubio, Elisa Iniesto, J. Salinas, 2017, Molecular plant.

SPR analysis of promoter binding of Synechocystis PCC6803 transcription factors NtcA and CRP suggests cross‐talk and sheds light on regulation by effector molecules

V. Rubio, K. Forchhammer, Alicia Forcada-Nadal, 2014, FEBS letters.

Mechanism of activation of bicarbonate ion by mitochondrial carbamoyl-phosphate synthetase: formation of enzyme-bound adenosine diphosphate from the adenosine triphosphate that yields inorganic phosphate.

V. Rubio, S. Grisolía, B. Sproat, 1981, Biochemistry.

Molecular Characterization of Carbamoyl‐Phosphate Synthetase (CPS1) Deficiency Using Human Recombinant CPS1 as a Key Tool

V. Rubio, I. Pérez-Arellano, M. Summar, 2013, Human mutation.