H. Leemhuis

发表

Directed evolution of enzymes: Library screening strategies

H. Leemhuis, R. Kelly, L. Dijkhuizen, 2009, IUBMB life.

Directed evolution of a histone acetyltransferase – enhancing thermostability, whilst maintaining catalytic activity and substrate specificity

H. Leemhuis, F. Hollfelder, K. Nightingale, 2008, The FEBS journal.

New genotype-phenotype linkages for directed evolution of functional proteins.

Viktor Stein, Florian Hollfelder, H. Leemhuis, 2005, Current opinion in structural biology.

Single amino acid mutations interchange the reaction specificities of cyclodextrin glycosyltransferase and the acarbose-modifying enzyme acarviosyl transferase.

H. Leemhuis, L. Dijkhuizen, U. Wehmeier, 2004, Biochemistry.

From macroscopic mechanics to cell-effective stiffness within highly aligned macroporous collagen scaffolds.

H. Leemhuis, I. Heschel, G. Duda, 2019, Materials science & engineering. C, Materials for biological applications.

Characterization of the GH13 and GH57 glycogen branching enzymes from Petrotoga mobilis SJ95 and potential role in glycogen biosynthesis

H. Leemhuis, M. van der Maarel, Xuewen Zhang, 2019, PloS one.

The thermostable 4,6-α-glucanotransferase of Bacillus coagulans DSM 1 synthesizes isomaltooligosaccharides

H. Leemhuis, M. van der Maarel, G. Xiang, 2021, Amylase.

Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution.

P. de Vos, H. Leemhuis, H. Schols, 2021, Carbohydrate polymers.

GtfC Enzyme of Geobacillus sp. 12AMOR1 Represents a Novel Thermostable Type of GH70 4,6-α-Glucanotransferase That Synthesizes a Linear Alternating (α1 → 6)/(α1 → 4) α-Glucan and Delays Bread Staling.

H. Leemhuis, L. Dijkhuizen, L. Oudhuis, 2021, Journal of agricultural and food chemistry.

University of Groningen Thermoanaerobacterium thermosulfurigenes cyclodextrin glycosyltransferase. Mechanism and kinetics of inhibition by acarbose and cyclodextrins Leemhuis,

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2002 .

Mutations converting cyclodextrin glycosyltransferase from a transglycosylase into a starch hydrolase

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2002, FEBS letters.

University of Groningen Hydrophobic amino acid residues in the acceptor binding site are main determinants for reaction mechanism and specificity of cyclodextrin-glycosyltransferase Veen,

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2001 .

Digestion kinetics of low, intermediate and highly branched maltodextrins produced from gelatinized starches with various microbial glycogen branching enzymes.

H. Leemhuis, M. van der Maarel, Xuewen Zhang, 2020, Carbohydrate polymers.

Isomalto/malto-polysaccharide, a novel soluble dietary fiber made via enzymatic conversion of starch.

H. Leemhuis, L. Dijkhuizen, J. Kamerling, 2014, Journal of agricultural and food chemistry.

Properties and applications of starch-converting enzymes of the α-amylase family

Lubbert Dijkhuizen, H. Leemhuis, L. Dijkhuizen, 2002 .

Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications

Lubbert Dijkhuizen, Ronan M. Kelly, H. Leemhuis, 2009, Applied Microbiology and Biotechnology.

Identification of acceptor substrate binding subsites +2 and +3 in the amylomaltase from Thermus thermophilus HB8.

H. Leemhuis, L. Dijkhuizen, B. A. van der Veen, 2007, Biochemistry.

Improved thermostability of bacillus circulans cyclodextrin glycosyltransferase by the introduction of a salt bridge

Lubbert Dijkhuizen, H. Leemhuis, L. Dijkhuizen, 2003, Proteins.

Properties and applications of starch-converting enzymes of the alpha-amylase family.

H. Leemhuis, L. Dijkhuizen, B. A. van der Veen, 2002, Journal of biotechnology.

Thermoanaerobacterium thermosulfurigenes cyclodextrin glycosyltransferase.

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2003, European journal of biochemistry.

Thermoanaerobacterium thermosulfurigenes cyclodextrin glycosyltransferase. Mechanism and kinetics of inhibition by acarbose and cyclodextrins

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2002 .

Starch modification with microbial alpha-glucanotransferase enzymes.

H. Leemhuis, M. van der Maarel, 2013, Carbohydrate polymers.

A GH57 4-α-glucanotransferase of hyperthermophilic origin with potential for alkyl glycoside production

E. Karlsson, H. Leemhuis, L. Dijkhuizen, 2015, Applied Microbiology and Biotechnology.

Characterization of the Gene Cluster Involved in Isoprene Metabolism in Rhodococcus sp. Strain AD45

H. Leemhuis, D. Janssen, J. H. Spelberg, 2000, Journal of bacteriology.

Inulin and levan synthesis by probiotic Lactobacillus gasseri strains: characterization of three novel fructansucrase enzymes and their fructan products.

H. Leemhuis, L. Dijkhuizen, S. Kralj, 2010, Microbiology.

Chapter 3 Biochemical characterization of Lactobacillus reuteri Glycoside Hydrolase family 70 GtfB type of 4 , 6-α-glucanotransferase enzymes that synthesize soluble dietary starch fibers

H. Leemhuis, L. Dijkhuizen, Zhengyu Jin, 2018 .

6-alpha-Glucanotransferase , a Novel Enzyme That Structurally and Functionally Provides an Evolutionary Link between Glycoside Hydrolase Enzyme Families

H. Leemhuis, L. Dijkhuizen, S. Kralj, 2018 .

Biochemical Characterization of the Lactobacillus reuteri Glycoside Hydrolase Family 70 GTFB Type of 4,6-α-Glucanotransferase Enzymes That Synthesize Soluble Dietary Starch Fibers

H. Leemhuis, L. Dijkhuizen, Zhengyu Jin, 2015, Applied and Environmental Microbiology.

Biochemical characterization of Lactobacillus reuteri Glycoside Hydrolase family 70 GTFB

H. Leemhuis, L. Dijkhuizen, Zhengyu Jin, 2015 .

Glucansucrases: three-dimensional structures, reactions, mechanism, α-glucan analysis and their implications in biotechnology and food applications.

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2013, Journal of biotechnology.

Glycosidic bond specificity of glucansucrases: on the role of acceptor substrate binding residues

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2012 .

4,6-α-Glucanotransferase activity occurs more widespread in Lactobacillus strains and constitutes a separate GH70 subfamily

H. Leemhuis, L. Dijkhuizen, T. Pijning, 2012, Applied Microbiology and Biotechnology.

4,6-α-Glucanotransferase, a Novel Enzyme That Structurally and Functionally Provides an Evolutionary Link between Glycoside Hydrolase Enzyme Families 13 and 70

H. Leemhuis, L. Dijkhuizen, S. Kralj, 2011, Applied and Environmental Microbiology.

4,6- (cid:1) -Glucanotransferase, a Novel Enzyme That Structurally and Functionally Provides an Evolutionary Link between Glycoside Hydrolase Enzyme Families 13 and 70 (cid:1)

H. Leemhuis, L. Dijkhuizen, S. Kralj, 2011 .

University of Groningen 4,6-alpha-Glucanotransferase, a Novel Enzyme That Structurally and Functionally Provides an Evolutionary Link between Glycoside Hydrolase Enzyme Families 13 and 70 Kralj,

H. Leemhuis, L. Dijkhuizen, S. Kralj, 2011 .

Conversion of cyclodextrin glycosyltransferase into a starch hydrolase by directed evolution: the role of alanine 230 in acceptor subsite +1.

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2003, Biochemistry.

The Remote Substrate Binding Subsite −6 in Cyclodextrin-glycosyltransferase Controls the Transferase Activity of the Enzyme via an Induced-fit Mechanism*

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2002, The Journal of Biological Chemistry.

Hydrophobic Amino Acid Residues in the Acceptor Binding Site Are Main Determinants for Reaction Mechanism and Specificity of Cyclodextrin-glycosyltransferase*

H. Leemhuis, L. Dijkhuizen, Joost C. M. Uitdehaag, 2001, The Journal of Biological Chemistry.

Higher Chain Length Distribution in Debranched Type‐3 Resistant Starches (RS3) Increases TLR Signaling and Supports Dendritic Cell Cytokine Production

P. de Vos, H. Leemhuis, L. Oudhuis, 2018, Molecular nutrition & food research.

Identification of Thermotoga maritima MSB8 GH57 α-amylase AmyC as a glycogen-branching enzyme with high hydrolytic activity

H. Leemhuis, Š. Janeček, T. Pijning, 2019, Applied Microbiology and Biotechnology.

Thermus thermophilus Glycoside Hydrolase Family 57 Branching Enzyme

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2010, The Journal of Biological Chemistry.

The evolution of cyclodextrin glucanotransferase product specificity

H. Leemhuis, R. Kelly, L. Dijkhuizen, 2009, Applied Microbiology and Biotechnology.

BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS Identification of ThermotogamaritimaMSB8 GH57 α-amylase AmyC as a glycogen-branching enzyme with high hydrolytic activity

H. Leemhuis, Š. Janeček, T. Pijning, 2019 .

Elimination of competing hydrolysis and coupling side reactions of a cyclodextrin glucanotransferase by directed evolution.

H. Leemhuis, R. Kelly, L. Dijkhuizen, 2008, The Biochemical journal.

Crystal structure of inulosucrase from Lactobacillus: insights into the substrate specificity and product specificity of GH68 fructansucrases.

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2011, Journal of molecular biology.

The role of conserved inulosucrase residues in the reaction and product specificity of Lactobacillus reuteri inulosucrase

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2012, The FEBS journal.

Synthesis of highly branched α-glucans with different structures using GH13 and GH57 glycogen branching enzymes.

H. Leemhuis, M. van der Maarel, Xuewen Zhang, 2019, Carbohydrate polymers.

A biomaterial with a channel-like pore architecture induces endochondral healing of bone defects

H. Leemhuis, I. Heschel, G. Duda, 2018, Nature Communications.

Structural characterization of linear isomalto-/malto-oligomer products synthesized by the novel GTFB 4,6-α-glucanotransferase enzyme from Lactobacillus reuteri 121.

H. Leemhuis, L. Dijkhuizen, S. Kralj, 2012, Glycobiology.

4,6-α-Glucanotransferase activity occurs more widespread in Lactobacillus strains and constitutes a separate GH70 subfamily

H. Leemhuis, L. Dijkhuizen, T. Pijning, 2012, Applied Microbiology and Biotechnology.

Gluco-oligomers initially formed by the reuteransucrase enzyme of Lactobacillus reuteri 121 incubated with sucrose and malto-oligosaccharides.

H. Leemhuis, L. Dijkhuizen, G. Gerwig, 2013, Glycobiology.

Conversion of a cyclodextrin glucanotransferase into an alpha-amylase

H. Leemhuis, R. Kelly, L. Dijkhuizen, 2018 .

Structural elements determining the transglycosylating activity of glycoside hydrolase family 57 glycogen branching enzymes

H. Leemhuis, M. van der Maarel, Gang Xiang, 2021, Proteins.

Starch and alpha-glucan acting enzymes, modulating their properties by directed evolution.

H. Leemhuis, R. Kelly, L. Dijkhuizen, 2009, Journal of biotechnology.

Evolution toward Small Molecule Inhibitor Resistance Affects Native Enzyme Function and Stability, Generating Acarbose-insensitive Cyclodextrin Glucanotransferase Variants*

H. Leemhuis, R. Kelly, L. Dijkhuizen, 2008, Journal of Biological Chemistry.

Soft tissue volume augmentation in the oral cavity with a collagen-based 3D matrix with orientated open pore structure

H. Leemhuis, I. Heschel, M. Tortorici, 2018, Current Directions in Biomedical Engineering.

Utilization of Indonesian root and tuber starches for glucose production by cold enzymatic hydrolysis

H. Leemhuis, Z. Nurachman, M. van der Maarel, 2023, Biologia.

Engineering cyclodextrin glycosyltransferase into a starch hydrolase with a high exo-specificity.

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2003, Journal of biotechnology.

Engineering of Hydrolysis Reaction Specificity in the Transglycosylase Cyclodextrin Glycosyltransferase

H. Leemhuis, L. Dijkhuizen, 2003 .

The fully conserved Asp residue in conserved sequence region I of the α‐amylase family is crucial for the catalytic site architecture and activity

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2003, FEBS letters.

Hydrolysis and Transglycosylation Reaction Specificity of Cyclodextrin Glycosyltransferases

H. Leemhuis, L. Dijkhuizen, 2003 .

A biomaterial with a channel-like pore architecture induces endochondral healing of bone defects

I Heschel, H. Leemhuis, I. Heschel, 2018, Nature Communications.

Conversion of a cyclodextrin glucanotransferase into an alpha-amylase: assessment of directed evolution strategies.

H. Leemhuis, R. Kelly, L. Dijkhuizen, 2007, Biochemistry.

Glucansucrases: three-dimensional structures, reactions, mechanism, α-glucan analysis and their implications in biotechnology and food applications.

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2013, Journal of biotechnology.

Crystal structure of inulosucrase from Lactobacillus: insights into the substrate specificity and product specificity of GH68 fructansucrases.

H. Leemhuis, L. Dijkhuizen, B. Dijkstra, 2011, Journal of molecular biology.

Inulin and levan synthesis by probiotic Lactobacillus gasseri strains: characterization of three novel fructansucrase enzymes and their fructan products.

H. Leemhuis, L. Dijkhuizen, S. Kralj, 2010, Microbiology.

The Human Histone Acetyltransferase P/CAF is a Promiscuous Histone Propionyltransferase

H. Leemhuis, F. Hollfelder, K. Nightingale, 2008, Chembiochem : a European journal of chemical biology.

Synthesis of highly branched alpha-glucans with different structures using GH13 and GH57 glycogen branching enzymes

H. Leemhuis, M. V. D. Maarel, Xuewen Zhang, 2019 .

Properties and applications of starch-converting enzymes of the alpha-amylase family.

H. Leemhuis, L. Dijkhuizen, J. Uitdehaag, 2002, Journal of biotechnology.