K. Nagai

发表

Crystallization of RNA-protein complexes.

E. Obayashi, C. Oubridge, D. P. Pomeranz Krummel, 2007, Methods in molecular biology.

Coordinated amino acid changes in homologous protein families.

K. Nagai, D. Altschuh, T. Vernet, 1988, Protein engineering.

Crystal structure at 1.92 Å resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin

Nobutoshi Ito, Philip R. Evans, Chris Oubridge, 1994, Nature.

RNA recognition by RNP proteins during RNA processing.

G. Varani, K. Nagai, 1998, Annual review of biophysics and biomolecular structure.

RNA-binding proteins: TRAPping RNA bases

C. Oubridge, K. Nagai, Y. Muto, 2000, Current Biology.

CryoEM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution

C. Oubridge, K. Nagai, S. Scheres, 2016, Nature.

Cryo-electron microscopy snapshots of the spliceosome: structural insights into a dynamic ribonucleoprotein machine

K. Nagai, S. Fica, 2017, Nature Structural &Molecular Biology.

RNA‐Binding Proteins

K. Nagai, S. Cusack, 2002 .

Recruiting proteins to the RNA world

K. Nagai, I. Mattaj, 1995, Nature Structural Biology.

The role of the distal histidine in myoglobin and haemoglobin

Jean-Paul Renaud, Stephen G. Sligar, Barry A. Springer, 1988, Nature.

Haemoglobin titusville: α94 Asp → Asn: A new haemoglobin with a lowered affinity for oxygen

K. Nagai, H. Lehmann, P. Lorkin, 1975 .

Gemin 6 and 7 lend a hand to snRNP assembly.

K. Nagai, A. Leung, 2005, Structure.

Cryo-EM Studies of Pre-mRNA Splicing: From Sample Preparation to Model Visualization.

K. Nagai, P. Lin, M. E. Wilkinson, 2018, Annual review of biophysics.

Resonance Raman Evidence for a Stretched Fe-Nε (His F8) Bond in the T-Structure Deoxyhemoglobin

K. Nagai, T. Kitagawa, 1982 .

Crystal structure of Prp8 reveals active site cavity of the spliceosome

C. Oubridge, K. Nagai, W. P. Galej, 2012, Nature.

Chemical Synthesis of U1 snRNA Derivatives

K. Nagai, M. Sekine, Y. Kondo, 2013, Organic letters.

Two structurally different RNA molecules are bound by the spliceosomal protein U1A using the same recognition strategy.

C. Oubridge, K. Nagai, L. Jovine, 1996, Structure.

Studies of haemoglobin functions by site-directed mutagenesis

M. Perutz, K. Nagai, C. Poyart, 1986, Philosophical Transactions of the Royal Society of London. Series A, Mathematical and Physical Sciences.

Assembly of the Human Signal Recognition Particle

K. Nagai, E. Menichelli, 2009 .

Structural basis for conformational equilibrium of the catalytic spliceosome

K. Nagai, W. P. Galej, S. Fica, 2020, bioRxiv.

A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation

C. Oubridge, K. Nagai, A. Newman, 2019, Science.

The search for drug target molecules from genomics

Y. Masuho, K. Nagai, K. Nagahari, 2001, BJU international.

Protein engineering as a tool for crystallography.

K. Nagai, S. Price, 1995, Current opinion in biotechnology.

Pigment chemistry: The red sweat of the hippopotamus

K. Nagai, K. Hashimoto, M. Nakata, 2004, Nature.

Production of human hemoglobin in Escherichia coli using cleavable fusion protein expression vector.

K. Nagai, B. Luisi, J. Tame, 1994, Methods in enzymology.

Reply to "Complex conformations and crystal contacts"

C. Oubridge, K. Nagai, A. Kuglstatter, 2003, Nature Structural Biology.

Differences in Fe(II)-N epsilon(His-F8) stretching frequencies between deoxyhemoglobins in the two alternative quaternary structures.

K. Nagai, T. Kitagawa, 1980, Proceedings of the National Academy of Sciences of the United States of America.

Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase.

K. Nagai, T. Ignjatovic, Ji-Chun Yang, 2005, Journal of molecular biology.

Structure of spliceosomal ribonucleoproteins

C. Oubridge, K. Nagai, D. P. Krummel, 2010, F1000 biology reports.

Structural studies of the spliceosome: blind men and an elephant.

K. Nagai, A. Newman, 2010, Current opinion in structural biology.

Resonance raman studies of hemoglobins M: evidence for iron-tyrosine charge-transfer interactions in the abnormal subunits of Hb M Boston and Hb M Iwate.

K. Nagai, T. Kitagawa, F. Taketa, 1983, Biochemistry.

Crystal structure of the U1A spliceosomal protein complexed with its cognate RNA hairpin.

P. Evans, C. Oubridge, K. Nagai, 1995, Nucleic acids symposium series.

A mutagenic study of the allosteric linkage of His(HC3)146β in haemoglobin

M. Perutz, K. Nagai, B. Luisi, 1993 .

Structural and functional consequences of amino acid substitutions in hemoglobin as manifested in natural and artificial mutants.

K. Nagai, J. Tame, K. Imai, 1989, Protein sequences & data analysis.

X-ray crystallographic and functional studies of human haemoglobin mutants produced in Escherichia coli.

M. Perutz, K. Nagai, B. Luisi, 1987, Acta haematologica.

Oxygen binding properties of human mutant hemoglobins synthesized in Escherichia coli.

M. Perutz, K. Nagai, C. Poyart, 1985, Proceedings of the National Academy of Sciences of the United States of America.

Solubilization of aster-forming proteins from yeast: possible constituents of spindle pole body and reconstitution of asters in vitro.

K. Nagai, M. Yamasaki, A. Yamamoto, 1990, Cell structure and function.

Assignment of proximal histidyl imidazole exchangeable proton NMR resonances to individual subunits in hemoglobins A, Boston, Iwate and Milwaukee.

K. Gersonde, K. Nagai, T. Jue, 1980, Biochemical and biophysical research communications.

The effects of E7 and E11 mutations on the kinetics of ligand binding to R state human hemoglobin.

K. Nagai, J. Tame, J. Olson, 1989, The Journal of biological chemistry.

Cryptic initiation sequence revealed

K. Nagai, 1990, Nature.

A human recombinant haemoglobin designed for use as a blood substitute

K. Nagai, N. Komiyama, A. Mathews, 1992, Nature.

Influence of globin structures on the state of the heme. Ferrous low spin derivatives.

M. Perutz, K. Nagai, J. Kilmartin, 1976, Biochemistry.

Influence of amino acid replacements in the heme pocket on the electron paramagnetic resonance spectra and absorption spectra of nitrosylhemoglobins M Iwate, M Boston, and M Milwaukee.

K. Nagai, H. Morimoto, H. Hori, 1979, Biochemistry.

The effect of quaternary structure on the state of the α and β subunits within nitrosyl haemoglobin: Low temperature photodissociation and the ESR spectra

K. Nagai, H. Morimoto, H. Hori, 1978 .

Deuterium nuclear magnetic resonance studies on the interaction of glycophorin with 1,2-dimyristoylamido-1,2-deoxyphosphatidylcholine.

K. Nagai, B. Lindman, J. Sunamoto, 1990, Biochimica et biophysica acta.

Synthesis and antifungal activities of (2R,3R)-2-aryl-1-azolyl-3-(substituted amino)-2-butanol derivatives as topical antifungal agents.

K. Nagai, T. Arika, H. Kobayashi, 1999, Chemical & pharmaceutical bulletin.

Interpreting a Low Resolution Map of Human U1 snRNP Using Anomalous Scatterers

C. Oubridge, K. Nagai, Jade Li, 2009, Structure.

Further characterization of the lipid-depleted bovine rhodopsin obtained by cholate-ammonium sulfate fractionation.

K. Nagai, Y. Ebina, M. Makino, 1980, Biochimica et biophysica acta.

Proton Magnetic Resonance Investigation of the Influence of Quaternary Structure on Iron-Histidine Bonding in Deoxyhemoglobins

K. Nagai, T. Jue, H. Bunn, 1982 .

Mutagenic Studies on the Origins of the Root Effect

K. Nagai, J. Tame, Sam-Yong Park, 2008 .

Quaternary structures and low frequency molecular vibrations of haems of deoxy and oxyhaemoglobin studied by resonance raman scattering.

K. Nagai, H. Morimoto, T. Kitagawa, 1980, Journal of molecular biology.

Effect of the distal residues on the vibrational modes of the Fe-CO bond in hemoglobin studied by protein engineering.

K. Nagai, J. Tame, J. Renaud, 1990, Biochemistry.

Assignment of the Fe—Nϵ (His F8) stretching band in the resonance Raman spectra of deoxy myoglobin

K. Nagai, T. Kitagawa, M. Tsubaki, 1979 .

Direct demonstration of the essential role of the intramolecular high-mannose oligosaccharide chains in the folding and assembly of soybean (glycine max) lectin polypeptides.

K. Nagai, H. Yamaguchi, 1993, Journal of biochemistry.

Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli

K. Nagai, H. Thøgersen, 1984, Nature.

RNA-protein complexes.

K. Nagai, 1996, Current opinion in structural biology.

Role of intramolecular high-mannose chains in the folding and assembly of soybean (Glycine max) lectin polypeptides: studies by the combined use of spectroscopy and gel-filtration size analysis.

K. Nagai, K. Shibata, H. Yamaguchi, 1993, Journal of biochemistry.

An integrated analysis and database system for full-length cDNA.

K. Nagai, T. Ota, T. Nishikawa, 2000, Genome informatics. Workshop on Genome Informatics.

Prespliceosome structure provides insights into spliceosome assembly and regulation

K. Nagai, P. Lin, C. Plaschka, 2018, Nature.

Structure of a pre-catalytic spliceosome

K. Nagai, P. Lin, Kiyoshi Nagai, 2017, Nature.

Mechanism of 5ʹ splice site transfer for human spliceosome activation

K. Nagai, M. E. Wilkinson, C. Charenton, 2019, Science.

Crystal structure of human spliceosomal U1 snRNP at 5.5 Å resolution

C. Oubridge, D. P. Pomeranz Krummel, K. Nagai, 2009, Nature.

Site-directed mutagenesis of the regulatory light-chain Ca2+/Mg2+ binding site and its role in hybrid myosins

K. Nagai, F. Reinach, J. Kendrick-jones, 1986, Nature.

Identification of essential amino acid residues of an alpha-amylase inhibitor from Phaseolus vulgaris white kidney beans.

K. Nagai, T. Nishimoto, Y. Wada, 1999, Journal of biochemistry.

Structure and assembly of the spliceosomal small nuclear ribonucleoprotein particles.

K. Nagai, C. Kambach, S. Walke, 1999, Current opinion in structural biology.

Crystal structure of human U1 snRNP, a small nuclear ribonucleoprotein particle, reveals the mechanism of 5′ splice site recognition

C. Oubridge, K. Nagai, Y. Kondo, 2015, eLife.

Molecular Mechanism and Evolution of Nuclear Pre-mRNA and Group II Intron Splicing: Insights from Cryo-Electron Microscopy Structures.

K. Nagai, W. P. Galej, A. Newman, 2018, Chemical reviews.

The structure and biochemical properties of the human spliceosomal protein U1C.

David Neuhaus, Chris Oubridge, Yutaka Muto, 2004, Journal of molecular biology.

Crystal structure of the RNA-binding domain of the U1 small nuclear ribonucleoprotein A

P. Evans, C. Oubridge, K. Nagai, 1990, Nature.

Crystal structure of the spliceosomal U2B″–U2A′ protein complex bound to a fragment of U2 small nuclear RNA

Philip R. Evans, P. Evans, K. Nagai, 1998, Nature.

Structure of a spliceosome remodelled for exon ligation

Max E. Wilkinson, Chris Oubridge, C. Oubridge, 2017, Nature.

CryoEM structure of the spliceosome immediately after branching

C. Oubridge, K. Nagai, W. P. Galej, 2016, Nature.

Assembly and dynamics of the U4/U6 di-snRNP by single-molecule FRET

K. Nagai, Y. Kondo, D. Rueda, 2015, Nucleic acids research.

RNA Splicing by the Spliceosome.

K. Nagai, M. E. Wilkinson, C. Charenton, 2019, Annual review of biochemistry.

Preparation of RNA:protein complexes for X-ray crystallography and NMR

G. Varani, C. Oubridge, K. Nagai, 1998 .

Zinc-finger motifs expressed in E. coli and folded in vitro direct specific binding to DNA

K. Nasmyth, K. Nagai, D. Rhodes, 1988, Nature.

RNA-protein interactions

K. Nagai, 1992 .

Crystal Structures of Two Sm Protein Complexes and Their Implications for the Assembly of the Spliceosomal snRNPs

K. Nagai, R. Lührmann, C. Kambach, 1999, Cell.

CryoEM structures of spliceosomal complexes reveal the molecular mechanism of pre-mRNA splicing.

K. Nagai, S. Scheres, 2017, Current opinion in structural biology.

Recognition of a signal peptide by the signal recognition particle

C. Oubridge, K. Nagai, C. Robinson, 2010, Nature.

psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation

J. Ule, N. Luscombe, C. Oubridge, 2020, bioRxiv.

Crystallographic analysis of mutant human haemoglobins made in Escherichia coli

K. Nagai, B. Luisi, 1986, Nature.

Induced structural changes of 7SL RNA during the assembly of human signal recognition particle

Chris Oubridge, C. Oubridge, K. Nagai, 2002, Nature Structural Biology.

The RNP domain: a sequence-specific RNA-binding domain involved in processing and transport of RNA.

P. Evans, C. Oubridge, K. Nagai, 1995, Trends in biochemical sciences.

The architecture of the spliceosomal U4/U6.U5 tri-snRNP

Sjors H. W. Scheres, Xiao-chen Bai, K. Nagai, 2015, Nature.

Structural Basis of Nuclear pre-mRNA Splicing: Lessons from Yeast.

K. Nagai, A. Newman, C. Plaschka, 2019, Cold Spring Harbor perspectives in biology.

Specificity of ribonucleoprotein interaction determined by RNA folding during complex formation

G. Varani, K. Nagai, F. Allain, 1996, Nature.

Transplanting a unique allosteric effect from crocodile into human haemoglobin

K. Nagai, J. Tame, N. Komiyama, 1995, Nature.

Was the loss of the D helix in α globin a functionally neutral mutation?

K. Nagai, J. Tame, N. Komiyama, 1991, Nature.

CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast

K. Nagai, P. Lin, Thi Hoang Duong Nguyen, 2016, Current opinion in structural biology.

Stoichiometry of the Sm proteins in yeast spliceosomal snRNPs supports the heptamer ring model of the core domain.

B. Séraphin, K. Nagai, E. Bragado‐Nilsson, 2001, Journal of molecular biology.

Resonance Raman spectra of myoglobins reconstituted with spirographis and isospirographis hemes and iron 2,4-diformylprotoporphyrin IX. Effect of formyl substitution at the heme periphery.

K. Nagai, T. Kitagawa, M. Tsubaki, 1980, Biochemistry.

Hb Helsinki: a Variant with a High Oxygen Affinity and a Substitution at a 2,3-DPG Binding Site (β82 [EF6] Lys→Met)

K. Nagai, H. Lehmann, P. Pikkarainen, 1976 .

Hb Helsinki: a variant with a high oxygen affinity and a substitution at a 2,3-DPG binding site (beta82[EF6] Lys replaced by Met).

K. Nagai, H. Lehmann, P. Pikkarainen, 1976, Acta haematologica.

Structural studies of the spliceosome: zooming into the heart of the machine☆

K. Nagai, Thi Hoang Duong Nguyen, W. P. Galej, 2014, Current opinion in structural biology.

Structural Basis of Brr2-Prp8 Interactions and Implications for U5 snRNP Biogenesis and the Spliceosome Active Site

K. Nagai, Thi Hoang Duong Nguyen, W. P. Galej, 2013, Structure.

Identification of molecular contacts between the U1 A small nuclear ribonucleoprotein and U1 RNA.

C. Oubridge, K. Nagai, C. Teo, 1991, The EMBO journal.

Solution structure of the N-terminal RNP domain of U1A protein: the role of C-terminal residues in structure stability and RNA binding.

G. Varani, K. Nagai, F. Allain, 1996, Journal of molecular biology.

Postcatalytic spliceosome structure reveals mechanism of 3′–splice site selection

K. Nagai, C. Norman, W. P. Galej, 2017, Science.

Recent advances in RNA-protein interaction studies

K. Nagai, 1993, Molecular Biology Reports.

Haemoglobin Titusville: alpha94 Asp replaced by Asn. A new haemoglobin with a lowered affinity for oxygen.

K. Nagai, H. Lehmann, P. Lorkin, 1975, Biochimica et biophysica acta.

Crystal structure of the ffh and EF-G binding sites in the conserved domain IV of Escherichia coli 4.5S RNA.

T. Sixma, C. Oubridge, K. Nagai, 2000, Structure.

113Cd NMR Experiments Reveal an Unusual Metal Cluster in the Solution Structure of the Yeast Splicing Protein Bud31p†

K. Nagai, Ji-Chun Yang, D. Neuhaus, 2015, Angewandte Chemie.

Oxygen dissociation curve of haemoglobin portland

K. Nagai, H. Lehmann, Soodsarkorn Tuchinda, 1975, FEBS letters.

Crystallization of RNA-protein complexes. I. Methods for the large-scale preparation of RNA suitable for crystallographic studies.

C. Oubridge, K. Nagai, N. Ito, 1995, Journal of molecular biology.

Crystal structure of SRP19 in complex with the S domain of SRP RNA and its implication for the assembly of the signal recognition particle.

Chris Oubridge, C. Oubridge, K. Nagai, 2002, Molecular cell.

Stereochemistry of carbon monoxide binding to normal human adult and Cowtown haemoglobins.

P. Emsley, M. Perutz, K. Nagai, 1990, Journal of molecular biology.

Functional role of the distal valine (E11) residue of α subunits in human haemoglobin

K. Nagai, J. Tame, G. Fermi, 1991 .

Proton magnetic resonance study of the influence of chemical modification, mutation, quaternary state, and ligation state on dynamic stability of the heme pocket in hemoglobin as reflected in the exchange of the proximal histidyl ring labile proton.

K. Nagai, K. Han, G. L. La Mar, 1989, Biochemistry.

Evolution of haemoglobin studied by protein engineering

K. Nagai, B. Luisi, D. Shih, 1988, BioEssays : news and reviews in molecular, cellular and developmental biology.

Evolution of Hemoglobin as Studied by Protein Engineering and X-Ray Crystallography

K. Nagai, D. Altschuh, J. Tame, 1988 .

1H-NMR heme resonance assignments by selective deuteration in low-spin complexes of ferric hemoglobin A.

K. Nagai, R. Kauten, R. Pandey, 1988, Biochimica et biophysica acta.

Distal residues in the oxygen binding site of haemoglobin studied by protein engineering

K. Nagai, B. Luisi, K. Imai, 1987, Nature.

Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain.

B. Séraphin, E. Obayashi, C. Oubridge, 2017, RNA.

Structure of the spliceosomal U4 snRNP core domain and its implication for snRNP biogenesis

K. Nagai, Jade Li, Kiyoshi Nagai, 2011, Nature.

Quaternary structure-induced photoreduction of haem of haemoglobin

K. Nagai, T. Kitagawa, 1979, Nature.

Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif

David Neuhaus, E. Obayashi, K. Nagai, 2008, Proceedings of the National Academy of Sciences.

Post-catalytic spliceosome structure reveals mechanism of 3'-splice site selection

K. Nagai, C. Norman, W. P. Galej, 2017, bioRxiv.

Secrets of RNA folding revealed.

K. Nagai, S. Price, 1996, Structure.

The structure of a domain of the group I intron, the largest structure of an RNA to date, reveals structural details of tertiary interactions required for the folding of a large RNA.

K. Nagai, S. Price, 1996 .

Structure and Assembly of the Spliceosomal snRNPs

Yutaka Muto, K. Nagai, T. Ignjatovic, 2001 .

Author response: Crystal structure of human U1 snRNP, a small nuclear ribonucleoprotein particle, reveals the mechanism of 5′ splice site recognition

C. Oubridge, K. Nagai, Y. Kondo, 2014 .

Structure, function and evolution of the signal recognition particle

Elena Menichelli, Chris Oubridge, C. Oubridge, 2003, The EMBO journal.

Functional properties of the glycosylated minor hemoglobins A1a-1,A1a-2 and A1b. EPR evidence for increased stability of the low affinity quaternary structure and decreased susceptibility to organic phosphate.

K. Nagai, H. Hori, A. Kaneko, 1980, Biochimica et Biophysica Acta.

The assignment of carbon monoxide association rate constants to the alpha and beta subunits in native and mutant human deoxyhemoglobin tetramers.

K. Nagai, J. Tame, J. Olson, 1991, The Journal of biological chemistry.

Use of RNA tertiary interaction modules for the crystallisation of the spliceosomal snRNP core domain.

K. Nagai, Y. Kondo, M. Jinek, 2010, Journal of molecular biology.

Structure and assembly of the spliceosomal snRNPs. Novartis Medal Lecture.

D. P. Pomeranz Krummel, K. Nagai, T. Ignjatovic, 2001, Biochemical Society transactions.

Recruiting more proteins to the RNA world

K. Nagai, W. Scott, 2018, Science.

A mutagenic study of the allosteric linkage of His(HC3)146 beta in haemoglobin.

M. Perutz, K. Nagai, B. Luisi, 1993, Journal of molecular biology.

RNA-Protein Interactions in Ribonucleoprotein Particles

K. Nagai, 1993 .

Protein-induced conformational changes of RNA during the assembly of human signal recognition particle.

C. Oubridge, K. Nagai, C. Isel, 2007, Journal of molecular biology.

Crystallisation of RNA-protein complexes. II. The application of protein engineering for crystallisation of the U1A protein-RNA complex.

C. Oubridge, K. Nagai, N. Ito, 1995, Journal of molecular biology.

Resonance Raman evidence for cleavage of the Fe-N epsilon(His-F8) bond in the alpha subunit of the T-structure nitrosylhemoglobin.

K. Nagai, T. Kitagawa, D. Dolphin, 1980, Biochemistry.

Effect of removal of a salt-bridge on the oxygen binding properties and the electronic structure of heme in cobalt-iron hybrid hemoglobin.

K. Nagai, M. Tsubaki, 1979, Journal of Biochemistry (Tokyo).

Crystallization and preliminary X-ray analysis of the conserved domain IV of Escherichia coli 4.5S RNA.

C. Oubridge, K. Nagai, L. Jovine, 2000, Acta crystallographica. Section D, Biological crystallography.

The effect of ferric ligands on the oxygen affinity of the ferrous subunits in valency hybrid haemoglobins.

K. Nagai, 1977, Journal of molecular biology.

NMR studies of U1 snRNA recognition by the N‐terminal RNP domain of the human U1A protein.

G. Varani, K. Nagai, D. Neuhaus, 1994, The EMBO journal.

The influence of quaternary structure on the EPR spectra of ferric haemoglobin

K. Nagai, H. Hori, 1978, FEBS letters.

Influence of quaternary structure on the state of the haem in carp and human methaemoglobins studied by resonance Raman scattering.

K. Nagai, T. Kitagawa, Y. Enoki, 1980, Biochimica et biophysica acta.