A. Kingsman

发表

Identification of a novel HIV-1 TAR RNA bulge binding protein.

B. Baker, M. Muckenthaler, E. Vivés, 1994, Nucleic acids research.

Real-time analysis of the transcriptional regulation of HIV and hCMV promoters in single mammalian cells.

M. Braddock, A. Kingsman, S. Kingsman, 1995, Journal of cell science.

Quantitative Localisation of HIV Proteins in Mammalian Cells

M. Braddock, A. Kingsman, S. Kingsman, 1993 .

HIV-1 TAT “activates” presynthesized RNA in the nucleus

Martin Braddock, A. Chambers, M. Braddock, 1989, Cell.

Recombinant DNA Techniques· 5

A. Kingsman, S. Kingsman, M. Dobson, 1991 .

Characterization of the equine infectious anaemia virus S2 protein.

A. Kingsman, S. Kingsman, K. Mitrophanous, 2000, The Journal of general virology.

Efficient expression of the Saccharomyces cerevisiae PGK gene depends on an upstream activation sequence but does not require TATA sequences

A. Kingsman, S. Kingsman, J. Ogden, 1986 .

Transcriptional control of the Saccharomyces cerevisiae PGK gene by RAP1

A. Chambers, A. Kingsman, S. Kingsman, 1989, Molecular and cellular biology.

Phosphorylation influences the binding of the yeast RAP1 protein to the upstream activating sequence of the PGK gene.

A. Chambers, A. Kingsman, S. Kingsman, 1990, Nucleic acids research.

Characterisation of the DNA binding domain of the yeast RAP1 protein.

A. Chambers, A. Kingsman, S. Kingsman, 1990, Nucleic acids research.

Replication in Saccharomyces cerevisiae of plasmid pBR313 carrying DNA from the yeast trpl region.

A. Kingsman, R. Mortimer, L. Clarke, 1979, Gene.

Inhibition of human immunodeficiency virus type 1 Tat-dependent activation of translation in Xenopus oocytes by the benzodiazepine Ro24-7429 requires trans-activation response element loop sequences

M. Muckenthaler, M. Braddock, A. Kingsman, 1994, Journal of virology.

Characterization of physiologically regulated vectors for the treatment of ischemic disease.

A. Kingsman, K. Boast, H. Spearman, 1999, Human gene therapy.

Human immunodeficiency virus type 1 integrase: effect on viral replication of mutations at highly conserved residues

A. Kingsman, S. Kingsman, P. Cannon, 1994, Journal of virology.

The requirements for conjugal DNA synthesis in the donor strain during flac transfer.

A. Kingsman, N. Willetts, 1978, Journal of molecular biology.

Hybrid human immunodeficiency virus Gag particles as an antigen carrier system: induction of cytotoxic T-cell and humoral responses by a Gag:V3 fusion

A. Kingsman, S. Adams, E. Berrie, 1993, Journal of virology.

Induction of high-titer neutralizing antibodies, using hybrid human immunodeficiency virus V3-Ty viruslike particles in a clinically relevant adjuvant

J. Moore, John P. Moore, A. Kingsman, 1991, Journal of virology.

Regulated high efficiency expression of human interferon‐alpha in Saccharomyces cerevisiae.

A. Kingsman, S. Kingsman, M. Tuite, 1982, The EMBO journal.

Ty: A retroelement moving forward

A. Kingsman, S. Kingsman, 1988, Cell.

An adenoviral vector regulated by hypoxia for the treatment of ischaemic disease and cancer

A. Kingsman, S. Kingsman, S. Naylor, 1999, Gene Therapy.

Complete nucleotide sequence of a mouse VL30 retro-element

M. Malim, A. Kingsman, S. Kingsman, 1988, Molecular and cellular biology.

HIV p24-specific helper T cell clones from immunized primates recognize highly conserved regions of HIV-1.

A. Kingsman, S. Kingsman, K. Mills, 1990, Journal of immunology.

Induction of single and dual cytotoxic T‐lymphocyte responses to viral proteins in mice using recombinant hybrid Ty–virus‐like particles

A. Kingsman, S. Adams, S. Woodrow, 1996, Immunology.

Induction of HIV-specific cytotoxic T lymphocytes in vivo with hybrid HIV-1 V3:Ty-virus-like particles.

A. Kingsman, A. Gearing, S. Adams, 1993, Journal of immunology.

Optimizing Transgene Configuration and Protein Fusions to Maximize Dopamine Production for the Gene Therapy of Parkinson's Disease.

A. Kingsman, S. Palfi, G. S. Ralph, 2016, Human gene therapy. Clinical development.

Expression in Xenopus oocytes.

A. Chambers, M. Braddock, A. Kingsman, 1990, Current opinion in biotechnology.

Efficient production of germline transgenic chickens using lentiviral vectors

A. Kingsman, A. Sherman, H. Sang, 2004, EMBO reports.

Possible regulatory function of the Saccharomyces cerevisiae Ty1 retrotransposon core protein

A. Kingsman, S. Kingsman, E. Martin‐Rendon, 2000 .

Reverse transcriptase activity and Ty RNA are associated with virus-like particles in yeast

M. Malim, A. Kingsman, S. Kingsman, 1985, Nature.

Ty, an endogenous retrovirus of yeast?

A. Kingsman, S. Kingsman, J. Mellor, 1986, Yeast.

31P nuclear-magnetic resonance saturation transfer measurements of flux between inorganic phosphate and ATP in yeast cells over-producing phosphoglycerate kinase

G. Radda, A. Kingsman, K. Brindle, 1986 .

Identification of proteolytic cleavage sites within the gag‐analogue protein of Ty1 virus‐like particles

A. Kingsman, S. Kingsman, G. Marfany, 1996, Molecular microbiology.

Structural determinants within the subunit protein of Ty1 virus‐like particles

A. Kingsman, S. Kingsman, G. Marfany, 1996, Molecular microbiology.

Conserved sequences in the carboxyl terminus of integrase that are essential for human immunodeficiency virus type 1 replication

A. Kingsman, S. Kingsman, P. Cannon, 1996, Journal of virology.

The expression of homologous and heterologous genes in yeast

A. Kingsman, S. Kingsman, C. Stanway, 2004, Antonie van Leeuwenhoek.

The production of mammalian proteins in Saccharomyces cerevisiae

A. Kingsman, S. Kingsman, J. Mellor, 1987 .

The structure of the cysCDHIJ region in unstable cysteine or methionine requiring mutants of Salmonella typhimurium

A. Kingsman, 1977, Molecular and General Genetics MGG.

Immunization of human HIV‐seronegative volunteers with recombinant p17/p24: Ty virus‐like particles elicits HIV‐1 p24‐specific cellular and humoral immune responses

Seamus J. Martin, A. Kingsman, J. Senior, 1993, AIDS.

Immunogenicity of the yeast recombinant p17/p24:Ty virus-like particles (p24-VLP) in healthy volunteers.

A. Kingsman, J. Weber, S. Adams, 1995, Vaccine.

Sequence variation in dispersed repetitive sequences in Saccharomyces cerevisiae.

A. C. Chinault, A. Kingsman, L. Clarke, 1981, Journal of molecular biology.

High-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene.

A. Chambers, A. Kingsman, S. Kingsman, 1990, Methods in enzymology.

Split-Intron Retroviral Vectors: Enhanced Expression with Improved Safety

A. Kingsman, S. Kingsman, M. Uden, 2000, Journal of Virology.

Sequence variation in the LEU2 region of the saccharomyces cerevisiae genome.

A. Kingsman, S. Kingsman, M. Dobson, 1981, Gene.

DETECTION OF E. COLI CLONES CONTAINING SPECIFIC YEAST GENES BY IMMUNOLOGICAL SCREENING

A. C. Chinault, A. Kingsman, J. Carbon, 1979 .

The expression of hybrid HIV:Ty virus-like particles in yeast

A. Kingsman, S. Kingsman, K. Gull, 1987, Nature.

Mutants of Salmonella typhimurium responding to cysteine or methionine: their nature and possible role in the regulation of cysteine biosynthesis.

D. A. Smith, A. Kingsman, M. A. Qureshi, 1975, Journal of general microbiology.

Enhanced proliferative cellular responses to HIV-1 V3 peptide and gp120 following immunization with V3:Ty virus-like particles.

A. Kingsman, A. Gearing, S. Adams, 1992, Immunology.

Expression in Saccharomyces cerevisiae of human interferon-alpha directed by the TRP1 5' region.

A. Kingsman, S. Kingsman, M. Tuite, 1983, Nucleic acids research.

An immunological analysis of Ty1 virus-like particle structure.

A. Kingsman, K. Gull, S. Adams, 1995, Virology.

The functions and relationships of Ty-VLP proteins in yeast reflect those of mammalian retroviral proteins

A. Kingsman, K. Gull, J. Mellor, 1987, Cell.

The complete amino acid sequence of yeast phosphoglycerate kinase.

A. Kingsman, S. Kingsman, M. Tuite, 1983, The Biochemical journal.

Retinoic acid receptor β2 promotes functional regeneration of sensory axons in the spinal cord

John Grist, Mimoun Azzouz, S. McMahon, 2006, Nature Neuroscience.

Functional dissection of the Moloney murine leukemia virus envelope protein gp70

A. Kingsman, S. Kingsman, Y. Bae, 1997, Journal of virology.

Multiple cooperative interactions constrain BPV-1 E2 dependent activation of transcription.

A. Kingsman, S. Kingsman, M. Sowden, 1989, Nucleic acids research.

Efficient activation of transcription in yeast by the BPV1 E2 protein.

A. Kingsman, S. Kingsman, M. Sowden, 1989, Nucleic acids research.

A novel method for the purification of HIV-1 p24 protein from hybrid Ty virus-like particles (Ty-VLPs).

A. Kingsman, S. Kingsman, K. Gull, 1989, AIDS.

A retrovirus-like strategy for expression of a fusion protein encoded by yeast transposon Ty1

A. Kingsman, S. Kingsman, J. Mellor, 1985, Nature.

Murine leukemia virus-based Tat-inducible long terminal repeat replacement vectors: a new system for anti-human immunodeficiency virus gene therapy

A. Kingsman, S. Kingsman, P. Cannon, 1996, Journal of virology.

Purification and secondary structure determination of simian immunodeficiency virus p27.

A. Kingsman, S. Kingsman, S. Adams, 1990, Journal of molecular biology.

Upstream and downstream transcriptional control signals in the yeast retrotransposon, TY.

A. Kingsman, S. Kingsman, P. Rathjen, 1988, Nucleic acids research.

Targets for Antiviral Chemotherapy: HIV Regulatory Proteins

A. Kingsman, S. Kingsman, 1990 .

MiniReview: Antiviral Target Series, Number 2 Targets for antiviral chemotherapy: HIV regulatory proteins

A. Kingsman, S. Kingsman, 1990 .

The control of transcription in Saccharomyces cerevisiae

A. Kingsman, S. Kingsman, C. Stanway, 1987, BioEssays : news and reviews in molecular, cellular and developmental biology.

Effects of stoichiometry of retroviral components on virus production.

A. Kingsman, S. Kingsman, M. Yap, 2000, The Journal of general virology.

Mutagenesis analysis of the murine leukemia virus matrix protein: identification of regions important for membrane localization and intracellular transport

A. Kingsman, S. Kingsman, Y. Soneoka, 1997, Journal of virology.

Heterologous gene expression in Saccharomyces cerevisiae.

A. Kingsman, S. Kingsman, J. Mellor, 1985, Biotechnology & genetic engineering reviews.

Frameshifting in Prokaryotes and Eukaryotes

A. Kingsman, S. Kingsman, H. Engelberg-Kulka, 1996 .

HIV pol Expression via a Ribosomal Frameshift

A. Kingsman, S. Kingsman, W. Wilson, 1990 .

HIV expression strategies: Ribosomal frameshifting is directed by a short sequence in both mammalian and yeast systems

Martin Braddock, M. Braddock, A. Kingsman, 1988, Cell.

Expression strategies of the yeast retrotransposon Ty: a short sequence directs ribosomal frameshifting.

M. Malim, A. Kingsman, S. Kingsman, 1986, Nucleic acids research.

Atomic resolution structure of Moloney murine leukemia virus matrix protein and its relationship to other retroviral matrix proteins.

D. Stuart, A. Kingsman, Z. Rao, 2002, Structure.

Stable gene transfer to the nervous system using a non-primate lentiviral vector

A. Kingsman, Soonsang Yoon, N. Mazarakis, 1999, Gene Therapy.

Retroelement particles as purification, presentation and targeting vehicles.

A. Kingsman, S. Kingsman, S. Adams, 1991, Trends in biotechnology.

Analysis of 4070A envelope levels in retroviral preparations and effect on target cell transduction efficiency.

A. Kingsman, J. Sutton, S. Kingsman, 2000, Human gene therapy.

The Ty transposon of Saccharomyces cerevisiae determines the synthesis of at least three proteins.

A. Kingsman, S. Kingsman, J. Mellor, 1985, Nucleic acids research.

A transient three-plasmid expression system for the production of high titer retroviral vectors.

A. Kingsman, S. Kingsman, G. Romano, 1995, Nucleic acids research.

The "putative" leucine zipper region of murine leukemia virus transmembrane protein (P15e) is essential for viral infectivity.

A. Kingsman, S. Kingsman, E. E. Ramsdale, 1996, Virology.

Rabies virus glycoprotein pseudotyping of lentiviral vectors enables retrograde axonal transport and access to the nervous system after peripheral delivery.

A. Kingsman, E. Carter, R. Barber, 2001, Human molecular genetics.

The yeast Ty element: Recent advances in the study of a model retro‐element

A. Kingsman, S. Kingsman, S. Adams, 1987, BioEssays : news and reviews in molecular, cellular and developmental biology.

Minimal Requirement for a Lentivirus Vector Based on Human Immunodeficiency Virus Type 1

A. Kingsman, S. Kingsman, K. Mitrophanous, 1998, Journal of Virology.

Mutational analysis of the putative receptor-binding domain of Moloney murine leukemia virus glycoprotein gp70.

A. Kingsman, S. Kingsman, B. Panda, 2000, Virology.

A Rev-Independent Human Immunodeficiency Virus Type 1 (HIV-1)-Based Vector That Exploits a Codon-Optimized HIV-1gag-pol Gene

A. Kingsman, S. Kingsman, K. Mitrophanous, 2000, Journal of virology.

The Genetic Organization of the Yeast Ty Element

M. Malim, A. Kingsman, S. Kingsman, 1987, Journal of Cell Science.

The regulation of human immunodeficiency virus type-1 gene expression.

A. Kingsman, S. Kingsman, 1996, European journal of biochemistry.

Multicistronic Lentiviral Vector-Mediated Striatal Gene Transfer of Aromatic l-Amino Acid Decarboxylase, Tyrosine Hydroxylase, and GTP Cyclohydrolase I Induces Sustained Transgene Expression, Dopamine Production, and Functional Improvement in a Rat Model of Parkinson's Disease.

A. Kingsman, E. Carter, R. Barber, 2002, The Journal of Neuroscience.

Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.

A. Kingsman, S. Kingsman, M. Tuite, 1982, Nucleic acids research.

Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles

A. Kingsman, H. Saibil, S. Fuller, 1997, Journal of virology.

Retrovirus and retrotransposon particles as antigen presentation and delivery systems.

A. Kingsman, S. Adams, 1995, Pharmaceutical biotechnology.

Hybrid Ty virus-like particles.

A. Kingsman, S. Adams, G. Layton, 1994, International reviews of immunology.

Polyvalent recombinant antigens: a new vaccine strategy.

A. Kingsman, S. Kingsman, A J Kingsman, 1988, Vaccine.

TRIP: a novel double stranded RNA binding protein which interacts with the leucine rich repeat of flightless I.

A. Kingsman, S. Kingsman, E. Brown, 1998, Nucleic acids research.

G. Barton, I. Campbell, A. Kingsman, 1994, Nature.

Synthesis of a gene for the HIV transactivator protein TAT by a novel single stranded approach involving in vivo gap repair.

M. Braddock, A. Kingsman, S. Kingsman, 1988, Nucleic acids research.

Efficient expression of the Saccharomyces cerevisiae PGK gene depends on an upstream activation sequence but does not require TATA sequences

A. Kingsman, S. Kingsman, J. Mellor, 1986, Molecular and cellular biology.

Factors affecting heterologous gene expression in Saccharomyces cerevisiae.

A. Kingsman, S. Kingsman, J. Mellor, 1985, Gene.

Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae.

A. Kingsman, S. Kingsman, M. Tuite, 1983, Gene.

The identification and high level expression of a protein encoded by the yeast Ty element.

A. Kingsman, S. Kingsman, J. Mellor, 1984, The EMBO journal.

Retrovirus Vectors in Gene Therapy: Targeting to Specific Cells

A. Kingsman, G. Romano, P. Cannon, 1996 .

Nucleic Acids Research

A. Kingsman, S. Kingsman, M. Tuite, 2009, Nucleic Acids Research.

Intron-less RNA injected into the nucleus of Xenopus oocytes accesses a regulated translation control pathway.

M. Muckenthaler, M. Braddock, A. Kingsman, 1994, Nucleic acids research.

Transcriptional activation by upstream activator sequences requires distinct interactions with downstream elements in the yeast TRP1 promoter

A. Kingsman, S. Kingsman, J. Mellor, 1991, Molecular and General Genetics MGG.

Structure of Equine Infectious Anemia Virus Matrix Protein

D. Stuart, A. Kingsman, Z. Rao, 2002, Journal of Virology.

Use of Intron-Disrupted Polyadenylation Sites To Enhance Expression and Safety of Retroviral Vectors

A. Kingsman, S. Kingsman, Said I. Ismail, 2001, Journal of Virology.

Promoter control of translation in Xenopus oocytes.

M. Muckenthaler, M. Braddock, A. Kingsman, 1995, Nucleic acids research.

Structure-function studies of the human immunodeficiency virus type 1 matrix protein, p17

A. Kingsman, S. Kingsman, P. Cannon, 1997, Journal of virology.

Cloning and characterization of hIF2, a human homologue of bacterial translation initiation factor 2, and its interaction with HIV-1 matrix.

A. Kingsman, S. Kingsman, C. Adamson, 1999, The Biochemical journal.

Variants within the yeast Ty sequence family encode a class of structurally conserved proteins.

J. Warmington, S. Oliver, A. Kingsman, 1985, Nucleic acids research.

A homologue of the human MSS1 gene, a positive modulator of HIV-1 gene expression, is massively expressed in Xenopus oocytes.

W. Nacken, A. Kingsman, S. Kingsman, 1995, Biochimica et biophysica acta.

Drosophila ARSs contain the yeast ARS consensus sequence and a replication enhancer.

A. Kingsman, S. Kingsman, J. Mills, 1986, Nucleic acids research.

ARS binding factor 1 binds adjacent to RAP1 at the UASs of the yeast glycolytic genes PGK and PYK1.

A. Chambers, A. Kingsman, S. Kingsman, 1990, Nucleic acids research.

The UAS of the yeast PGK gene is composed of multiple functional elements.

A. Chambers, A. Kingsman, S. Kingsman, 1988, Nucleic acids research.

Multiple control elements in the TRP1 promoter of Saccharomyces cerevisiae

A. Kingsman, S. Kingsman, J. Mellor, 1986, Molecular and cellular biology.

Characterization of the transcriptional potency of sub-elements of the UAS of the yeast PGK gene in a PGK mini-promoter.

A. Chambers, A. Kingsman, S. Kingsman, 1989, Nucleic acids research.

Proliferative Responses to the V3 Region of HIV Envelope Are Enhanced Following Immunization with V3:Ty Virus-Like Particles in Mice

A. Kingsman, S. Kingsman, A. Gearing, 1992 .

Multiple cis-active elements in the long control region of bovine papillomavirus type 1 (BPV-1).

A. Kingsman, S. Kingsman, S. Kim, 1987, Nucleic Acids Research.

An AT rich region of dyad symmetry is a promoter element in the yeast TRP1 gene

A. Kingsman, S. Kingsman, J. Mellor, 1988, Molecular and General Genetics MGG.

Transcriptional Control oftheSaccharomyces cerevisiae PGK GenebyRAPI

A. Kingsman, H. Tsang, 1989 .

Towards the Structure of Mosaic Proteins: Use of Protein Expression and NMR Techniques

I. Campbell, A. Kingsman, S. Kingsman, 1990 .

The UAS of the yeast PGK gene contains functionally distinct domains.

A. Kingsman, S. Kingsman, J. Mellor, 1987, Nucleic acids research.

A nuclear translational block imposed by the HIV-1 U3 region is relieved by the Tat-TAR interaction

A. Chambers, M. Braddock, A. Kingsman, 1990, Cell.

A heat shock element in the phosphoglycerate kinase gene promoter of yeast.

M. W. Davies, P. Piper, A. Kingsman, 1988, Nucleic acids research.

A transcriptional activator is located in the coding region of the yeast PGK gene.

A. Kingsman, S. Kingsman, J. Mellor, 1987, Nucleic acids research.

The effects of adjuvants on CTL induction by V3:Ty-virus-like particles (V3-VLPs) in mice.

A. Kingsman, A. Gearing, S. Adams, 1996, Vaccine.

Production and purification of hybrid Ty-VLPs

A. Kingsman, S. Kingsman, S. Adams, 1994, Molecular biotechnology.

Production and Purification of Hybrid Ty-VLPs.

A. Kingsman, S. Kingsman, S. Adams, 1992, Methods in molecular biology.

Requirement for HIV-1 TAR sequences for Tat activation in rodent cells.

M. Braddock, A. Kingsman, S. Kingsman, 1995, Virology.

Orientation-specific cis complementation by bulge- and loop-mutated human immunodeficiency virus type 1 TAR RNAs

M. Braddock, A. Kingsman, S. Kingsman, 1994, Journal of virology.

Retroviral protease-like sequence in the yeast transposon Ty 1(reply)

A. Kingsman, S. Kingsman, J. Mellor, 1985, Nature.

An adenosine at position 27 in the human immunodeficiency virus type 1 trans-activation response element is not critical for transcriptional or translational activation by Tat

A. Blanchard, M. Braddock, A. Kingsman, 1992, Journal of virology.

Refined solution structure of p17, the HIV matrix protein.

I. Campbell, A. Kingsman, S. Kingsman, 1995, Biochemical Society Transactions.

Reconstruction of the yeast 2 micron plasmid partitioning mechanism.

A. Kingsman, S. Kingsman, M. Molina, 1988, Nucleic acids research.

Yeast Ty retrotransposons assemble into virus-like particles whose T-numbers depend on the C-terminal length of the capsid protein.

A. Kingsman, H. Saibil, D. Bhella, 1999, Journal of molecular biology.

Characterization of human chromosomal DNA sequences which replicate autonomously in Saccharomyces cerevisiae.

A. Kingsman, S. Kingsman, M. Tuite, 1984, Nucleic acids research.

Blocking of Tat-dependent HIV-1 RNA modification by an inhibitor of RNA polymerase II processivity

M. Braddock, A. Kingsman, S. Kingsman, 1991, Nature.

Expression vectors for the construction of hybrid Ty-VLPs

A. Kingsman, S. Kingsman, S. Adams, 1994, Molecular biotechnology.

Expression Vectors for the Construction of Hybrid Ty-VLPs.

A. Kingsman, S. Kingsman, S. Adams, 1992, Methods in molecular biology.

Identification of immunodominant regions and linear B cell epitopes of the gE envelope protein of varicella-zoster virus.

D. Jeffries, A. Kingsman, S. Adams, 1995, Virology.

Symmetry, flexibility and permeability in the structure of yeast retrotransposon virus‐like particles.

A. Kingsman, S. Kingsman, H. Saibil, 1992, The EMBO journal.

Yeast Retrotransposon Particles as Antigen Delivery Systems

A. Kingsman, S. Adams, G. Layton, 1995, Annals of the New York Academy of Sciences.

HIV‐1 TAR RNA‐binding proteins control TAT activation of translation in Xenopus oocytes

A. Blanchard, M. Braddock, A. Kingsman, 1993, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

Regenerative properties of retinoic acid receptor beta 2 in rat model of dorsal rhizotomy

S. McMahon, A. Kingsman, N. Mazarakis, 2004 .

Host-vector systems.

A. Kingsman, S. Kingsman, 1989, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

The yeast ROAM mutation--identification of the sequences mediating host gene activation and cell-type control in the yeast retrotransposon, Ty.

A. Kingsman, S. Kingsman, P. Rathjen, 1987, Nucleic acids research.