J. Puglisi

发表

Translation at the single-molecule level.

Colin Echeverría Aitken, R. Marshall, M. Dorywalska, 2008, Annual review of biochemistry.

Absorbance melting curves of RNA.

I. Tinoco, J. Puglisi, 1989, Methods in enzymology.

RNA folding: Pseudoknots, loops and bulges

I. Tinoco, J. Puglisi, J. Wyatt, 1989, BioEssays : news and reviews in molecular, cellular and developmental biology.

Conformation of the TAR RNA-arginine complex by NMR spectroscopy.

J. Puglisi, J. Williamson, A. Frankel, 1992, Science.

RNA pseudoknots. Stability and loop size requirements.

I. Tinoco, J. Puglisi, J. Wyatt, 1990, Journal of molecular biology.

The molecular choreography of protein synthesis: translational control, regulation, and pathways

J. Puglisi, Jin Chen, Junhong Choi, 2016, Quarterly Reviews of Biophysics.

Dynamics of IRES-mediated translation

J. Puglisi, A. Petrov, Rosslyn Grosely, 2017, Philosophical Transactions of the Royal Society B: Biological Sciences.

Conformation of an RNA pseudoknot

I. Tinoco, J. Puglisi, J. Wyatt, 1990, Journal of Molecular Biology.

A pseudoknotted RNA oligonucleotide

I. Tinoco, J. Puglisi, J. Wyatt, 1988, Nature.

RNA structure from A to Z.

C. C. Hardin, I. Tinoco, J. Puglisi, 1987, Cold Spring Harbor symposia on quantitative biology.

tRNA selection and kinetic proofreading in translation

J. Puglisi, R. L. Gonzalez, Harold D. Kim, 2004, Nature Structural &Molecular Biology.

Ligand-specific regulation of the extracellular surface of a G protein coupled receptor

L. Pardo, L. Mueller, J. Puglisi, 2009, Nature.

An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments.

Colin Echeverría Aitken, R. Marshall, J. Puglisi, 2008, Biophysical journal.

The dynamics of SecM-induced translational stalling.

J. Puglisi, Jin Chen, M. Johansson, 2014, Cell reports.

Coupling of mRNA Structure Rearrangement to Ribosome Movement during Bypassing of Non-coding Regions

J. Puglisi, J. F. Atkins, Jin Chen, 2015, Cell.

The Pathway of HCV IRES-Mediated Translation Initiation

Joseph D. Puglisi, G. Otto, J. Puglisi, 2004, Cell.

tRNA dynamics on the ribosome during translation.

Steven Chu, Ruben L Gonzalez, Joseph D Puglisi, 2004, Proceedings of the National Academy of Sciences of the United States of America.

Solution Structure of a Bovine Immunodeficiency Virus Tat-TAR Peptide-RNA Complex

J. Puglisi, A. Frankel, S. Blanchard, 1995, Science.

N6-methyladenosine in mRNA disrupts tRNA selection and translation elongation dynamics

M. Ehrenberg, J. Puglisi, G. Rechavi, 2016, Nature Structural &Molecular Biology.

Structure of the A Site of Escherichia coli 16S Ribosomal RNA Complexed with an Aminoglycoside Antibiotic

J. Puglisi, D. Fourmy, S. Blanchard, 1996, Science.

Paromomycin binding induces a local conformational change in the A-site of 16 S rRNA.

J. Puglisi, D. Fourmy, S. Yoshizawa, 1998, Journal of molecular biology.

Concentric-Flow Electrokinetic Injector Enables Serial Crystallography of Ribosome and Photosystem-II

Sébastien Boutet, Nicholas K Sauter, Andrew Aquila, 2015, Nature Methods.

Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts

J. Puglisi, Olivier Duss, J. Williamson, 2018, Nature Communications.

eIF5B gates the transition from translation initiation to elongation

Christopher P. Lapointe, J. Puglisi, Dong-Hua Chen, 2019, Nature.

eIF5B gates the transition from translation initiation to elongation

Christopher P. Lapointe, J. Puglisi, Dong-Hua Chen, 2019, Nature.

Biochemical and NMR studies of RNA conformation with an emphasis on RNA pseudoknots.

J. Puglisi, J. Wyatt, 1995, Methods in enzymology.

Involvement of protein IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor

T. Steitz, J. Puglisi, C. Gualerzi, 2013, Proceedings of the National Academy of Sciences.

Mechanisms that ensure speed and fidelity in eukaryotic translation termination

R. Green, J. Puglisi, M. R. Lawson, 2021, Science.

Single-Molecule Fluorescence Applied to Translation.

J. Puglisi, Arjun Prabhakar, E. V. Puglisi, 2018, Cold Spring Harbor perspectives in biology.

Probing the Translation Dynamics of Ribosomes Using Zero-Mode Waveguides.

J. Puglisi, S. Uemura, Albert Tsai, 2016, Progress in molecular biology and translational science.

Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation

Christopher P. Lapointe, J. Puglisi, I. S. Fernández, 2021, Proceedings of the National Academy of Sciences.

eIF5B and eIF1A remodel human translation initiation complexes to mediate ribosomal subunit joining

Christopher P. Lapointe, J. Puglisi, Nancy Y. Villa, 2021, bioRxiv.

Fluorescently-tagged human eIF3 for single-molecule spectroscopy

J. Puglisi, Karim Majzoub, Junhong Choi, 2017, Nucleic acids research.

Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket.

V. Pande, J. Puglisi, D. Cane, 2012, Journal of the American Chemical Society.

Dynamic pathways of -1 translational frameshifting

J. Puglisi, Jin Chen, S. O’Leary, 2014, Nature.

Basis for prokaryotic specificity of action of aminoglycoside antibiotics

J. Puglisi, S. Douthwaite, M. Recht, 1999, The EMBO journal.

Cotranslational Protein Fo lding inside the Ribosome

G. Heijne, J. Puglisi, Annika Müller-Lucks, 2015 .

Role of RNA structure in arginine recognition of TAR RNA.

J. Puglisi, J. Williamson, A. Frankel, 1993, Proceedings of the National Academy of Sciences of the United States of America.

Application of Residual Dipolar Coupling Measurements To Identify Conformational Changes in RNA Induced by Antibiotics

J. Puglisi, S. R. Lynch, 2000 .

Transient Protein-RNA Interactions Guide Nascent Ribosomal RNA Folding

J. Puglisi, Olivier Duss, J. Williamson, 2019, Cell.

2′-O-methylation in mRNA disrupts tRNA decoding during translation elongation

M. Ehrenberg, J. Puglisi, Chuan He, 2018, Nature Structural & Molecular Biology.

Structural basis for the transition from translation initiation to elongation by an 80S-eIF5B complex

J. Puglisi, I. S. Fernández, B. Shin, 2020, Nature Communications.

eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining

Christopher P. Lapointe, J. Puglisi, I. S. Fernández, 2022, Nature.

Structure determination of large biological RNAs.

J. Puglisi, P. Lukavsky, 2005, Methods in enzymology.

Stabilization of Z-RNA by chemical bromination and its recognition by anti-Z-DNA antibodies.

C. C. Hardin, M. Trulson, I. Tinoco, 1987, Biochemistry.

Short translational ramp determines efficiency of protein synthesis

Hani S. Zaher, J. Puglisi, D. Piston, 2019, bioRxiv.

How mRNA and nascent chain sequences regulate translation elongation

Christopher P. Lapointe, Jinfan, J. Puglisi, 2019 .

Thiostrepton inhibition of tRNA delivery to the ribosome.

J. Puglisi, S. Chu, R. Gonzalez, 2007, RNA.

Dynamics of the context-specific translation arrest by chloramphenicol and linezolid

J. Puglisi, Dong-Hua Chen, A. Mankin, 2019, Nature Chemical Biology.

Post-termination Ribosome Intermediate Acts as the Gateway to Ribosome Recycling.

J. Puglisi, Junhong Choi, Arjun Prabhakar, 2017, Cell reports.

Multiple Parallel Pathways of Translation Initiation on the CrPV IRES.

J. Puglisi, Junya Chen, S. O’Leary, 2016, Molecular cell.

Three tRNAs on the ribosome slow translation elongation

J. Puglisi, Junhong Choi, 2017, Proceedings of the National Academy of Sciences.

A simple real-time assay for in vitro translation

J. Puglisi, A. Petrov, Guy L Kornberg, 2015, RNA.

NMR study of 100 kDa HCV IRES RNA using segmental isotope labeling.

J. Puglisi, Insil Kim, P. Lukavsky, 2002, Journal of the American Chemical Society.

RNA purification by preparative polyacrylamide gel electrophoresis.

J. Puglisi, A. Petrov, E. V. Puglisi, 2013, Methods in enzymology.

RPS 25 is required for efficient RAN translation of C 9 orf 72 and other 3 neurodegenerative disease-associated nucleotide repeats 4 5

L. Petrucelli, L. Partridge, J. Puglisi, 2019 .

Uncovering translation roadblocks during the development of a synthetic tRNA

M. Ehrenberg, J. Puglisi, D. Söll, 2022, Nucleic acids research.

Signal Recognition Particle-ribosome Binding Is Sensitive to Nascent Chain Length*

Margaret M. Elvekrog, J. Puglisi, P. Walter, 2014, The Journal of Biological Chemistry.

Length Binding Is Sensitive to Nascent Chain Signal Recognition Particle-ribosome Cell Biology:

J. Puglisi, P. Walter, S. Neher, 2014 .

Mechanisms that ensure speed and fidelity in eukaryotic translation termination

R. Green, J. Puglisi, Arjun Prabhakar, 2021, Science.

Real-time observation of signal recognition particle binding to actively translating ribosomes

J. Puglisi, P. Walter, Thomas R. Noriega, 2014, eLife.

Solution structure and proposed domain–domain recognition interface of an acyl carrier protein domain from a modular polyketide synthase

Chaitan Khosla, Joseph D Puglisi, J. Puglisi, 2007, Protein science : a publication of the Protein Society.

A memory of eS25 loss drives resistance phenotypes

Christopher P. Lapointe, Ryan A. Flynn, J. Puglisi, 2020, Nucleic acids research.

“A memory of RPS25 loss drives resistance phenotypes”

Christopher P. Lapointe, Ryan A. Flynn, J. Puglisi, 2019, bioRxiv.

Cotranslational Protein Folding inside the Ribosome Exit Tunnel

G. von Heijne, J. Puglisi, Rickard Hedman, 2015, Cell reports.

A short translational ramp determines the efficiency of protein synthesis

Hani S. Zaher, J. Puglisi, D. Piston, 2019, Nature Communications.

De novo computational RNA modeling into cryo-EM maps of large ribonucleoprotein complexes

Rhiju Das, Georgios Skiniotis, Kalli Kappel, 2018, Nature Methods.

RNA sequence determinants for aminoglycoside binding to an A-site rRNA model oligonucleotide.

J. Puglisi, K. Dahlquist, D. Fourmy, 1996, Journal of molecular biology.

Raman spectroscopic study of left-handed Z-RNA.

M. Trulson, I. Tinoco, R. Mathies, 1987, Biochemistry.

Solution conformation of an RNA hairpin loop.

I. Tinoco, J. Puglisi, J. Wyatt, 1990, Biochemistry.

Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts

J. Puglisi, Olivier Duss, J. Williamson, 2018, Nature Communications.

The impact of aminoglycosides on the dynamics of translation elongation.

Colin Echeverría Aitken, J. Korlach, M. Ehrenberg, 2013, Cell reports.

Influence of tRNA tertiary structure and stability on aminoacylation by yeast aspartyl-tRNA synthetase

Richard Giegé, Joseph D. Puglisi, Joern Putz, 1993, Nucleic Acids Res..

Structural origins of gentamicin antibiotic action

J. Puglisi, D. Fourmy, S. Yoshizawa, 1998, The EMBO journal.

Binding of neomycin-class aminoglycoside antibiotics to the A-site of 16 S rRNA.

J. Puglisi, D. Fourmy, M. Recht, 1998, Journal of molecular biology.

Preparation of isotopically labeled ribonucleotides for multidimensional NMR spectroscopy of RNA.

J. Puglisi, R. Batey, J. Williamson, 1992, Nucleic acids research.

Sequence-specific recognition of the major groove of RNA by deoxystreptamine.

J. Puglisi, D. Fourmy, S. Yoshizawa, 2002, Biochemistry.

Aminoglycoside Antibiotics and Decoding

J. Puglisi, K. Dahlquist, D. Fourmy, 2000 .

Probing the conformation of human tRNA3 Lys in solution by NMR

J. Puglisi, E. V. Puglisi, 2007, FEBS letters.

HIV-1 A-rich RNA loop mimics the tRNA anticodon structure

J. Puglisi, E. V. Puglisi, 1998, Nature Structural Biology.

Comparison of X-ray crystal structure of the 30S subunit-antibiotic complex with NMR structure of decoding site oligonucleotide-paromomycin complex.

J. Puglisi, S. R. Lynch, R. Gonzalez, 2003, Structure.

Purification and characterization of transcribed RNAs using gel filtration chromatography

Colin Echeverría Aitken, R. Marshall, J. Puglisi, 2007, Nature Protocols.

Large-scale preparation and purification of polyacrylamide-free RNA oligonucleotides.

Joseph D Puglisi, J. Puglisi, Peter J Lukavsky, 2004, RNA.

Synthesis and purification of large amounts of RNA oligonucleotides.

J. Puglisi, J. Wyatt, M. Chastain, 1991, BioTechniques.

The anti-hepatitis C agent nitazoxanide induces phosphorylation of eukaryotic initiation factor 2alpha via protein kinase activated by double-stranded RNA activation.

J. Puglisi, M. Elazar, Michael Liu, 2009, Gastroenterology.

Single Molecule Studies of Prokaryotic Translation

Colin Echeverría Aitken, R. Marshall, M. Dorywalska, 2009 .

De novo computational RNA modeling into cryo-EM maps of large ribonucleoprotein complexes

Rui Zhao, Rhiju Das, Kalli Kappel, 2018, Nature Methods.

The noncoding RNAs SNORD50A and SNORD50B bind K-Ras and are recurrently deleted in human cancer

J. Puglisi, Francesca Meschi, Z. Siprashvili, 2015, Nature Genetics.

RACK1 on and off the ribosome

Christopher P. Lapointe, J. Puglisi, Junhong Choi, 2019, bioRxiv.

Analysis of RNA by analytical polyacrylamide gel electrophoresis.

J. Puglisi, A. Petrov, A. Tsa, 2013, Methods in enzymology.

Architecture of an HIV-1 reverse transcriptase initiation complex

J. Puglisi, G. Skiniotis, K. Kappel, 2018, Nature.

GTP hydrolysis by IF2 guides progression of the ribosome into elongation.

Colin Echeverría Aitken, R. Marshall, J. Puglisi, 2009, Molecular cell.

Heterogeneous Pathways and Timing of Factor Departure during Translation Initiation

Sotaro Uemura, Jonas Korlach, Joseph D. Puglisi, 2012, Nature.

Initiation factor 2, tRNA, and 50S subunits cooperatively stabilize mRNAs on the ribosome during initiation

J. Puglisi, S. Uemura, T. Funatsu, 2012, Proceedings of the National Academy of Sciences.

Peptide bond formation destabilizes Shine–Dalgarno interaction on the ribosome

Sotaro Uemura, Steven Chu, Tae-Hee Lee, 2007, Nature.

Interaction of translation initiation factor IF1 with the E. coli ribosomal A site.

J. Puglisi, K. Dahlquist, 2000, Journal of molecular biology.

Mechanism of ribosome stalling during translation of a poly(A) tail

J. Puglisi, R. Hegde, V. Ramakrishnan, 2019, Nature Structural & Molecular Biology.

Structures of two RNA domains essential for hepatitis C virus internal ribosome entry site function

G. Otto, J. Puglisi, P. Sarnow, 2000, Nature Structural Biology.

The energy landscape of −1 ribosomal frameshifting

J. Puglisi, J. F. Atkins, Junhong Choi, 2020, Science Advances.

Coordinated conformational and compositional dynamics drive ribosome translocation

Joseph D. Puglisi, J. Puglisi, Jin Chen, 2013, Nature Structural &Molecular Biology.

Single-molecule imaging of full protein synthesis by immobilized ribosomes

H. Taguchi, J. Puglisi, S. Uemura, 2008, Nucleic acids research.

Large ribosomal subunit, eIF5B, Met-tRNAiMet and mRNA cooperate to complete accurate initiation

J. Puglisi, I. S. Fernández, B. Shin, 2020, bioRxiv.

Following the intersubunit conformation of the ribosome during translation in real time

Joseph D. Puglisi, Colin Echeverría Aitken, J. Puglisi, 2010, Nature Structural &Molecular Biology.

Site-specific labeling of the ribosome for single-molecule spectroscopy

Ruben L. Gonzalez, Steven Chu, Scott C. Blanchard, 2005, Nucleic acids research.

Structure of HCV IRES domain II determined by NMR

Joseph D Puglisi, G. Otto, J. Puglisi, 2003, Nature Structural Biology.

A short translational ramp determines the efficiency of protein synthesis

Hani S. Zaher, J. Puglisi, D. Piston, 2019, Nature Communications.

Title: Short translational ramp determines efficiency of protein synthesis Working title: Role of the first five codons on the processivity of translation

Hani S. Zaher, J. Puglisi, D. Piston, 2019 .

Multiplexed single-molecule analysis of human telomerase synthesizing DNA

Christopher P. Lapointe, Michael D. Stone, J. Puglisi, 2021, bioRxiv.

RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats

L. Petrucelli, L. Partridge, J. Puglisi, 2019, Nature Neuroscience.

Real-time tRNA transit on single translating ribosomes at codon resolution

Colin Echeverría Aitken, S. Turner, J. Korlach, 2010, Nature.

High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence

Jin Chen, Ravindra V Dalal, Alexey N Petrov, 2013, Proceedings of the National Academy of Sciences.

Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation

Christopher P. Lapointe, J. Puglisi, I. S. Fernández, 2020, Proceedings of the National Academy of Sciences.

Recognition of the codon-anticodon helix by ribosomal RNA.

J. Puglisi, D. Fourmy, S. Yoshizawa, 1999, Science.

Single ribosome dynamics and the mechanism of translation.

Joseph D Puglisi, Colin Echeverría Aitken, J. Puglisi, 2010, Annual review of biophysics.

Nonfluorescent quenchers to correlate single-molecule conformational and compositional dynamics.

Joseph D Puglisi, J. Puglisi, Jin Chen, 2012, Journal of the American Chemical Society.

Molecular Framework for the Activation of RNA-dependent Protein Kinase*♦

J. Puglisi, Corey W. Liu, G. Wagner, 2007, Journal of Biological Chemistry.

Quantitative polysome analysis identifies limitations in bacterial cell-free protein synthesis.

Joseph D Puglisi, J. Puglisi, J. Swartz, 2005, Biotechnology and bioengineering.

Dynamic basis of fidelity and speed in translation: Coordinated multistep mechanisms of elongation and termination

J. Puglisi, Junhong Choi, Arjun Prabhakar, 2017, Protein science : a publication of the Protein Society.

Polysomes Bypass a 50-Nucleotide Coding Gap Less Efficiently Than Monosomes Due to Attenuation of a 5′ mRNA Stem–Loop and Enhanced Drop-off

Patrick B. F. O'Connor, J. Weissman, J. Puglisi, 2020, Journal of Molecular Biology.

Polysomes Bypa Coding Gap Les Monosomes Due a 5′ mRNA Stem Enhanced Drop-

Patrick B. F. O'Connor, J. Weissman, J. Puglisi, 2022 .

Dynamics of the translational machinery.

Sotaro Uemura, Joseph D Puglisi, J. Puglisi, 2011, Current opinion in structural biology.

Structure of a eukaryotic decoding region A-site RNA.

J. Puglisi, S. R. Lynch, Stephen R. Lynch, 2001, Journal of molecular biology.

Structural origins of aminoglycoside specificity for prokaryotic ribosomes.

J. Puglisi, J D Puglisi, S. R. Lynch, 2001, Journal of molecular biology.

Dynamic Interplay of RNA and Protein in the Human Immunodeficiency Virus-1 Reverse Transcription Initiation Complex.

J. Puglisi, Junhong Choi, E. V. Puglisi, 2018, Journal of molecular biology.

Heterogeneous structures formed by conserved RNA sequences within the HIV reverse transcription initiation site

J. Puglisi, Aaron T. Coey, K. Larsen, 2016, RNA.

Secondary structure of the HIV reverse transcription initiation complex by NMR.

J. Puglisi, E. V. Puglisi, 2011, Journal of molecular biology.

PKR: A NMR perspective

Colin Echeverría Aitken, J. Puglisi, Corey W. Liu, 2007 .

Specific recognition of HIV TAR RNA by the dsRNA binding domains (dsRBD1-dsRBD2) of PKR.

J. Puglisi, Corey W. Liu, Insil Kim, 2006, Journal of molecular biology.

Dynamic recognition of the mRNA cap by Saccharomyces cerevisiae eIF4E.

J. Puglisi, Junya Chen, S. O’Leary, 2013, Structure.

mRNA processing: The 3′-end justifies the means

J. Puglisi, 2000, Nature Structural Biology.

Nuclear Magnetic Resonance Spectroscopy of RNA

J. Puglisi, E. V. Puglisi, 1998 .

Ribosomal proteins mediate the hepatitis C virus IRES-HeLa 40S interaction.

G. Otto, J. Puglisi, P. Sarnow, 2002, RNA.

RNAPack: an integrated NMR approach to RNA structure determination.

J. Puglisi, J D Puglisi, P J Lukavsky, 2001, Methods.

Partial spontaneous intersubunit rotations in actively translating ribosomes

J. Puglisi, Junhong Choi, Arjun Prabhakar, 2020, bioRxiv.

Amino acid sequence repertoire of the bacterial proteome and the occurrence of untranslatable sequences

J. Puglisi, N. Adir, Jin Chen, 2016, Proceedings of the National Academy of Sciences.

Sequence-dependent elongation dynamics on macrolide-bound ribosomes.

Magnus Johansson, Joseph D Puglisi, J. Puglisi, 2014, Cell reports.

Irreversible chemical steps control intersubunit dynamics during translation

Joseph D. Puglisi, R. Marshall, M. Dorywalska, 2008, Proceedings of the National Academy of Sciences.

The role of fluctuations in tRNA selection by the ribosome

J. Puglisi, Harold D. Kim, S. Chu, 2007, Proceedings of the National Academy of Sciences.

Rapid 40S scanning and its regulation by mRNA structure during eukaryotic translation initiation

J. Puglisi, Carlos Alvarado, J. Bohlen, 2021, Cell.

Fluctuations of transfer RNAs between classical and hybrid states.

Steven Chu, Joseph D Puglisi, J. Puglisi, 2007, Biophysical journal.

Z-RNA: A Left-Handed Double Helix

C. C. Hardin, T. Neilson, I. Tinoco, 1986 .

Mechanism of ribosome stalling during translation of a poly(A) tail

J. Puglisi, R. Hegde, V. Ramakrishnan, 2019, Nature Structural & Molecular Biology.

Approaching translation at atomic resolution

Scott C. Blanchard, Joseph D. Puglisi, Rachel Green, 2000, Nature Structural Biology.

Viral dsRNA inhibitors prevent self-association and autophosphorylation of PKR.

Colin Echeverría Aitken, J. Puglisi, D. Lindhout, 2007, Journal of molecular biology.

How 2 '-O-Methylation in mRNA Disrupts tRNA Decoding during Translation Elongation

M. Ehrenberg, J. Puglisi, G. Rechavi, 2018 .

Synthetic biology: Ribosomal ties that bind

J. Puglisi, 2015, Nature.

Single-molecule analysis of translational dynamics.

J. Puglisi, Junya Chen, S. O’Leary, 2012, Cold Spring Harbor perspectives in biology.

Unraveling the dynamics of ribosome translocation.

J. Puglisi, Junya Chen, S. O’Leary, 2012, Current opinion in structural biology.

Site-specific labeling of Saccharomyces cerevisiae ribosomes for single-molecule manipulations

J. Puglisi, A. Petrov, 2010, Nucleic acids research.

Rapid purification of RNAs using fast performance liquid chromatography (FPLC).

Joseph D Puglisi, J. Puglisi, S. McKenna, 2006, RNA.

Solution structure and backbone dynamics of the holo form of the frenolicin acyl carrier protein.

Chaitan Khosla, Joseph D Puglisi, J. Puglisi, 2003, Biochemistry.

N 6-Methyladenosines in mRNAs reduce the accuracy of codon reading by transfer RNAs and peptide release factors

M. Ehrenberg, J. Puglisi, Arjun Prabhakar, 2021, Nucleic acids research.

Kinetic pathway of 40S ribosomal subunit recruitment to hepatitis C virus internal ribosome entry site

J. Puglisi, J. Carette, P. Sarnow, 2014, Proceedings of the National Academy of Sciences.

The Diversity of Nuclear Magnetic Resonance Spectroscopy

Ronald W. Davis, K. Garcia, D. Herschlag, 2009 .

Biophysical and biochemical investigations of dsRNA-activated kinase PKR.

J. Puglisi, D. Lindhout, S. McKenna, 2007, Methods in enzymology.

How Messenger RNA and Nascent Chain Sequences Regulate Translation Elongation.

Christopher P. Lapointe, J. Puglisi, Junhong Choi, 2018, Annual review of biochemistry.

Uncoupling of RNA binding and PKR kinase activation by viral inhibitor RNAs.

J. Puglisi, Corey W. Liu, S. McKenna, 2006, Journal of molecular biology.

Relating Structure and Dynamics in RNA Biology.

J. Puglisi, Junhong Choi, Arjun Prabhakar, 2019, Cold Spring Harbor perspectives in biology.

The ribosome revealed

R. Green, J. Puglisi, 1999, Nature Structural Biology.

PAROMOMYCIN BINDING INDUCES A LOCAL CONFORMATIONAL CHANGE IN THE A SITE OF 16S RRNA, NMR, 20 STRUCTURES

J. Puglisi, D. Fourmy, S. Yoshizawa, 1998 .

Structure of a conserved RNA component of the peptidyl transferase centre

H. Noller, R. Green, J. Puglisi, 1997, Nature Structural Biology.

Dynamics of release factor recycling during translation termination in bacteria

M. Ehrenberg, J. Puglisi, Arjun Prabhakar, 2023, Nucleic acids research.

Solution mapping of T cell receptor docking footprints on peptide-MHC

David M. Kranz, K. Christopher Garcia, Joseph D. Puglisi, 2007, Proceedings of the National Academy of Sciences.

Design of a cyclic peptide that targets a viral RNA.

J. Puglisi, Steven T. Runyon, 2003, Journal of the American Chemical Society.

Solution structure of the A loop of 23S ribosomal RNA

J. Puglisi, S. Blanchard, J D Puglisi, 2001, Proceedings of the National Academy of Sciences of the United States of America.

rRNA chemical groups required for aminoglycoside binding.

J. Puglisi, D. Fourmy, S. Blanchard, 1998, Biochemistry.

Expanding single-molecule fluorescence spectroscopy to capture complexity in biology.

J. Puglisi, Junhong Choi, Rosslyn Grosely, 2019, Current opinion in structural biology.

Molecular Insights into PKR Activation by Viral Double-Stranded RNA

Colin Echeverría Aitken, J. Puglisi, D. Lindhout, 2007 .

NMR analysis of tRNA acceptor stem microhelices: discriminator base change affects tRNA conformation at the 3' end.

J. Puglisi, J. Williamson, E. V. Puglisi, 1994, Proceedings of the National Academy of Sciences of the United States of America.

Resolving the elegant architecture of the ribosome.

J. Puglisi, 2009, Molecular cell.

Structural biology: The dance of domains

J. Puglisi, 2007, Nature.

Universal features of Nsp1-mediated translational shutdown by coronaviruses

Christopher P. Lapointe, J. Puglisi, N. Ban, 2023, bioRxiv.

eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining

Christopher P. Lapointe, J. Puglisi, Nancy Y. Villa, 2022 .

Structural Characterization of the HIV-1 Reverse Transcriptase Initiation Complex

J. Puglisi, G. Skiniotis, K. Kappel, 2018 .

Structure and Biophysics - New Technologies for Current Challenges in Biology and Beyond

J. Puglisi, 2007 .

Real-time monitoring of single-molecule translation

Sotaro Uemura, Joseph D. Puglisi, J. Puglisi, 2011 .

Effect of mutations in the A site of 16 S rRNA on aminoglycoside antibiotic-ribosome interaction.

J. Puglisi, K. Dahlquist, S. Douthwaite, 1999, Journal of molecular biology.

Aminoglycoside Resistance with Homogeneous and Heterogeneous Populations of Antibiotic-Resistant Ribosomes

Joseph D. Puglisi, Michael I. Recht, J. Puglisi, 2001, Antimicrobial Agents and Chemotherapy.

De novo computational RNA modeling into cryo-EM maps of large ribonucleoprotein complexes

Rhiju Das, J. Puglisi, G. Skiniotis, 2018, Nature Methods.

RNA purification by preparative polyacrylamide gel electrophoresis.

J. Puglisi, A. Petrov, E. V. Puglisi, 2013, Methods in enzymology.

Using NMR to Determine the Conformation of the HIV Reverse Transcription Initiation Complex

J. Puglisi, E. V. Puglisi, 2012 .

Irreversible chemical steps control intersubunit dynamics during translation

R. Marshall, M. Dorywalska, J. Puglisi, 2008, Proceedings of the National Academy of Sciences.

Dynamic basis of fidelity and speed in translation: Coordinated multistep mechanisms of elongation and termination

J. Puglisi, Junhong Choi, Arjun Prabhakar, 2017, Protein science : a publication of the Protein Society.

Partial spontaneous intersubunit rotations in pretranslocation ribosomes

J. Puglisi, Junhong Choi, Arjun Prabhakar, 2023, Proceedings of the National Academy of Sciences of the United States of America.

Additive, cooperative and anti‐cooperative effects between identity nucleotides of a tRNA.

J. Puglisi, C. Florentz, R. Giegé, 1993, The EMBO journal.

Translational insensitivity to potent activation of PKR by HCV IRES RNA.

J. Puglisi, D. Lindhout, S. McKenna, 2009, Antiviral research.