H. Hellinga

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Conversion of a maltose receptor into a zinc biosensor by computational design

J. Marvin, H. Hellinga, 2001, Proceedings of the National Academy of Sciences of the United States of America.

Construction of new ligand binding sites in proteins of known structure. I. Computer-aided modeling of sites with pre-defined geometry.

F. Richards, H. Hellinga, 1991, Journal of molecular biology.

Computational design of receptor and sensor proteins with novel functions

L. Looger, H. Hellinga, M. Dwyer, 2003, Nature.

Structural reorganization and preorganization in enzyme active sites: comparisons of experimental and theoretically ideal active site geometries in the multistep serine esterase reaction cycle.

H. Hellinga, D. Hilvert, K. Houk, 2008, Journal of the American Chemical Society.

Programmable Ligand Detection System in Plants through a Synthetic Signal Transduction Pathway

Mauricio S. Antunes, C. Webb, H. Hellinga, 2011, PloS one.

Dissection of the protein G B1 domain binding site for human IgG Fc fragment

H W Hellinga, H. Hellinga, D. J. Sloan, 1999, Protein science : a publication of the Protein Society.

Engineering key components in a synthetic eukaryotic signal transduction pathway

Homme W Hellinga, Mauricio S. Antunes, Colleen T Webb, 2009, Molecular systems biology.

An empirical model for electrostatic interactions in proteins incorporating multiple geometry‐dependent dielectric constants

Homme W Hellinga, H. Hellinga, M. Wisz, 2003, Proteins.

Design of Bioelectronic Interfaces by Exploiting Hinge-Bending Motions in Proteins

David W. Conrad, David E. Benson, Scott A. Trammell, 2001, Science.

Integration of cell‐free protein coexpression with an enzyme‐linked immunosorbent assay enables rapid analysis of protein–protein interactions directly from DNA

Curtis J. Layton, H. Hellinga, Curtis J Layton, 2011, Protein science : a publication of the Protein Society.

Visualization of Synaptic Inhibition with an Optogenetic Sensor Developed by Cell-Free Protein Engineering Automation

H. Hellinga, G. Augustine, Lei Wen, 2013, The Journal of Neuroscience.

Multifactorial determinants of protein expression in prokaryotic open reading frames.

H. Hellinga, Malin J. Allert, J. Cox, 2010, Journal of molecular biology.

Construction of a fluorescent biosensor family

L. Looger, H. Hellinga, M. Dwyer, 2002, Protein science : a publication of the Protein Society.

Protein engineering and the development of generic biosensors.

J. Marvin, H. Hellinga, 1998, Trends in biotechnology.

Periplasmic binding proteins: a versatile superfamily for protein engineering.

H. Hellinga, M. Dwyer, 2004, Current opinion in structural biology.

Quantitation of protein–protein interactions by thermal stability shift analysis

Homme W Hellinga, Curtis J. Layton, H. Hellinga, 2011, Protein science : a publication of the Protein Society.

Computational design of a Zn2+ receptor that controls bacterial gene expression

L L Looger, H W Hellinga, L. Looger, 2003, Proceedings of the National Academy of Sciences of the United States of America.

Computational Design of a Biologically Active Enzyme

Loren L Looger, Homme W Hellinga, L. Looger, 2004, Science.

Structural analysis, identification, and design of calcium‐binding sites in proteins

Wei Yang, Hsiau-Wei Lee, H. Hellinga, 2002, Proteins.

Structure-based engineering of environmentally sensitive fluorophores for monitoring protein-protein interactions.

H. Hellinga, D. J. Sloan, 1998, Protein engineering.

The construction of metal centers in proteins by rational design.

H W Hellinga, H. Hellinga, 1998, Folding & design.

Rational design of nascent metalloenzymes.

H W Hellinga, D. E. Benson, H. Hellinga, 2000, Proceedings of the National Academy of Sciences of the United States of America.

Converting a maltose receptor into a nascent binuclear copper oxygenase by computational design.

D. E. Benson, H. Hellinga, A. Haddy, 2002, Biochemistry.

ITR/ACS+IM Computational Geometry for Structural Biology and Bioinformatics

M. Levitt, J. Latombe, P. Agarwal, 2002 .

Structure-Guided Discovery of Potent Antifungals that Prevent Ras Signaling by Inhibiting Protein Farnesyltransferase.

H. Hellinga, M. Distefano, L. Beese, 2022, Journal of medicinal chemistry.

Construction of a Blue Copper Analogue through Iterative Rational Protein Design Cycles Demonstrates Principles of Molecular Recognition in Metal Center Formation

Homme W. Hellinga, H. Hellinga, 1998 .

Structural adaptations that modulate monosaccharide, disaccharide, and trisaccharide specificities in periplasmic maltose-binding proteins.

Homme W Hellinga, H. Hellinga, L. Beese, 2009, Journal of molecular biology.

Ligand-induced conformational changes in a thermophilic ribose-binding protein

Homme W Hellinga, H. Hellinga, L. Beese, 2008, BMC Structural Biology.

The crystal structure of human protein farnesyltransferase reveals the basis for inhibition by CaaX tetrapeptides and their mimetics

H. Hellinga, L. Beese, A. Kral, 2001, Proceedings of the National Academy of Sciences of the United States of America.

Crystal structures of the fungal pathogen Aspergillus fumigatus protein farnesyltransferase complexed with substrates and inhibitors reveal features for antifungal drug design

H. Hellinga, L. Beese, N. Lubock, 2014, Protein science : a publication of the Protein Society.

Structures of Cryptococcus neoformans Protein Farnesyltransferase Reveal Strategies for Developing Inhibitors That Target Fungal Pathogens*

H. Hellinga, L. Beese, Shannon M. Kelly, 2011, The Journal of Biological Chemistry.

Structural evidence for the rare tautomer hypothesis of spontaneous mutagenesis

H. Hellinga, L. Beese, Weina Wang, 2011, Proceedings of the National Academy of Sciences.

Structural Factors That Determine Selectivity of a High Fidelity DNA Polymerase for Deoxy-, Dideoxy-, and Ribonucleotides*

H. Hellinga, L. Beese, Weina Wang, 2012, The Journal of Biological Chemistry.

Describing complex structure-function relationships in biomolecules at equilibrium.

Malin J Allert, Homme W Hellinga, H. Hellinga, 2020, Journal of molecular biology.

Structural Analysis of a Periplasmic Binding Protein in the Tripartite ATP-independent Transporter Family Reveals a Tetrameric Assembly That May Have a Role in Ligand Transport*

H. Hellinga, L. Beese, M. Cuneo, 2008, Journal of Biological Chemistry.

The backbone structure of the thermophilic Thermoanaerobacter tengcongensis ribose binding protein is essentially identical to its mesophilic E. coli homolog.

H. Hellinga, M. Cuneo, Malin J. Allert, 2008, BMC structural biology.

A miniaturized technique for assessing protein thermodynamics and function using fast determination of quantitative cysteine reactivity

T. Oas, H. Hellinga, D. Isom, 2011, Proteins.

Structural Analysis of Semi-specific Oligosaccharide Recognition by a Cellulose-binding Protein of Thermotoga maritima Reveals Adaptations for Functional Diversification of the Oligopeptide Periplasmic Binding Protein Fold*

H. Hellinga, L. Beese, M. Cuneo, 2009, The Journal of Biological Chemistry.

Structure‐based design of robust glucose biosensors using a Thermotoga maritima periplasmic glucose‐binding protein

H. Hellinga, L. Beese, B. Höcker, 2007, Protein science : a publication of the Protein Society.

Generalized dead-end elimination algorithms make large-scale protein side-chain structure prediction tractable: implications for protein design and structural genomics.

L L Looger, H W Hellinga, L. Looger, 2001, Journal of molecular biology.

Computational protein engineering

H. W. Hellinga, H. Hellinga, 1998, Nature Structural Biology.

Construction of a novel redox protein by rational design: conversion of a disulfide bridge into a mononuclear iron-sulfur center.

H W Hellinga, D. E. Benson, H. Hellinga, 1998, Biochemistry.

Interplay of catalysis, fidelity, threading, and processivity in the exo- and endonucleolytic reactions of human exonuclease I

H. Hellinga, L. Beese, Yuqian Shi, 2017, Proceedings of the National Academy of Sciences.

Discovery of Thermostable, Fluorescently Responsive Glucose Biosensors by Structure-Assisted Function Extrapolation.

H. Hellinga, Malin J. Allert, Malin J Allert, 2022, Biochemistry.

Construction of new ligand binding sites in proteins of known structure. II. Grafting of a buried transition metal binding site into Escherichia coli thioredoxin.

F M Richards, H W Hellinga, F. Richards, 1991, Journal of molecular biology.

VisualizationofSynapticInhibitionwithanOptogenetic SensorDevelopedbyCell-FreeProteinEngineering Automation

HommeW . Hellinga, Duke-NUSGraduateMedicalSchool Disorders, H. Hellinga, 2013 .

NMR studies of structure, hydrogen exchange, and main‐chain dynamics in a disrupted‐core mutant of thioredoxin

H. Hellinga, L. Spicer, R. Lorimier, 1996, Protein science : a publication of the Protein Society.

Picomole-scale characterization of protein stability and function by quantitative cysteine reactivity

Homme W Hellinga, Terrence G Oas, Eyal Vardy, 2010, Proceedings of the National Academy of Sciences.

Control of a biomolecular motor-powered nanodevice with an engineered chemical switch

Loren L Looger, Homme W Hellinga, George D Bachand, 2002, Nature materials.

Identifying and Designing of Calcium Binding Sites in Proteins by Computational Algorithm

H. Hellinga, Jenny J. Yang, Wei Yang, 2002 .

Thermodynamic analysis of ligand-induced changes in protein thermal unfolding applied to high-throughput determination of ligand affinities with extrinsic fluorescent dyes.

H. Hellinga, Curtis J Layton, 2010, Biochemistry.

Thermodynamic effects of reduction of the active-site disulfide of Escherichia coli thioredoxin explored by differential scanning calorimetry.

H. Hellinga, J. Ladbury, R. Wynn, 1994, Biochemistry.

Site-directed mutagenesis in the effector site of Escherichia coli phosphofructokinase.

A. Fersht, P. Evans, H. Hellinga, 1987, Biochemistry.

Mutations in the active site of Escherichia coli phosphofructokinase

P. Evans, H. Hellinga, 1987, Nature.

Nucleotide sequence and high-level expression of the major Escherichia coli phosphofructokinase.

P. Evans, H. Hellinga, Philip R. Evans, 1985, European journal of biochemistry.

Local encoding of computationally designed enzyme activity.

Homme W Hellinga, H. Hellinga, M. Dwyer, 2007, Journal of molecular biology.

Protein fabrication automation

Homme W Hellinga, H. Hellinga, J. Cox, 2007, Protein science : a publication of the Protein Society.

Structures of Human Exonuclease 1 DNA Complexes Suggest a Unified Mechanism for Nuclease Family

H. Hellinga, L. Beese, P. Modrich, 2011, Cell.

Optimal sequence selection in proteins of known structure by simulated evolution.

F M Richards, H W Hellinga, F. Richards, 1994, Proceedings of the National Academy of Sciences of the United States of America.

Manipulation of ligand binding affinity by exploitation of conformational coupling

Homme W. Hellinga, J. Marvin, H. Hellinga, 2001, Nature Structural Biology.

The rational design of allosteric interactions in a monomeric protein and its applications to the construction of biosensors.

H W Hellinga, J. Marvin, H. Hellinga, 1997, Proceedings of the National Academy of Sciences of the United States of America.

The rational design and construction of a cuboidal iron-sulfur protein.

H W Hellinga, H. Hellinga, C. Coldren, 1997, Proceedings of the National Academy of Sciences of the United States of America.

Hellinga reversible thiol protection mechanisms Orthogonal site-specific protein modification by engineering

H. Hellinga, M. Cuneo, J. Smith, 2004 .

Thermally controlled intein splicing of engineered DNA polymerases provides a robust and generalizable solution for accurate and sensitive molecular diagnostics

H. Hellinga, L. Beese, Yuqian Shi, 2023, Nucleic acids research.

Mapping staphylococcal nuclease conformation using an EDTA-Fe derivative attached to genetically engineered cysteine residues.

H. Hellinga, G. Hsu, M. Ermácora, 1994, Biochemistry.

Identification of cognate ligands for the Escherichia coli phnD protein product and engineering of a reagentless fluorescent biosensor for phosphonates

H. Hellinga, M. Cuneo, Shahir S. Rizk, 2006, Protein science : a publication of the Protein Society.

The crystal structure of a thermophilic glucose binding protein reveals adaptations that interconvert mono and di-saccharide binding sites.

H. Hellinga, L. Beese, M. Cuneo, 2006, Journal of molecular biology.

Integrated optical waveguide attenuated total reflection spectrometry and resonance Raman spectroscopy of adsorbed cytochrome c

William M. Reichert, Steven S Saavedra, Homme W. Hellinga, 1993 .

Computational design of receptors for an organophosphate surrogate of the nerve agent soman.

Loren L Looger, Homme W Hellinga, L. Looger, 2004, Proceedings of the National Academy of Sciences of the United States of America.

Engineering Biosensors by Introducing Fluorescent Allosteric Signal Transducers: Construction of a Novel Glucose Sensor

Homme W. Hellinga, J. Marvin, H. Hellinga, 1998 .

Orthogonal site‐specific protein modification by engineering reversible thiol protection mechanisms

H. Hellinga, M. Cuneo, D. Conrad, 2005, Protein science : a publication of the Protein Society.

Stability of oxidized Escherichia coli thioredoxin and its dependence on protonation of the aspartic acid residue in the 26 position.

H. Hellinga, J. Ladbury, R. Wynn, 1993, Biochemistry.

Biosensor for the detection of glucose

L. Looger, H. Hellinga, 2003 .

Genetic engineering of a single-chain antibody fragment for surface immobilization in an optical biosensor.

H W Hellinga, W M Reichert, W. Reichert, 1998, Biosensors & bioelectronics.

Analysis of allosteric signal transduction mechanisms in an engineered fluorescent maltose biosensor

D. E. Benson, L. Looger, H. Hellinga, 2005, Protein science : a publication of the Protein Society.

Rational design of a calcium-binding protein.

Wei Yang, Homme W Hellinga, Hsiau-Wei Lee, 2003, Journal of the American Chemical Society.

Harnessing environmental Ca2+ for extracellular protein thermostabilization.

H. Hellinga, Malin J. Allert, Malin J Allert, 2020, Biochemistry.

Rationally engineered meganucleases with altered sequence specificity and DNA binding affinity

H. Hellinga, J. J. Smith, D. Jantz, 2006 .

Rational protein design: combining theory and experiment.

H W Hellinga, H. Hellinga, 1997, Proceedings of the National Academy of Sciences of the United States of America.

Binding and signaling of surface‐immobilized reagentless fluorescent biosensors derived from periplasmic binding proteins

H. Hellinga, Yaji Tian, Homme W. Hellinga, 2006, Protein science : a publication of the Protein Society.

Design of a calcium-binding protein with desired structure in a cell adhesion molecule.

Wei Yang, Homme W Hellinga, H. Hellinga, 2005, Journal of the American Chemical Society.

Construction of a catalytically active iron superoxide dismutase by rational protein design.

H W Hellinga, H. Hellinga, J P Caradonna, 1997, Proceedings of the National Academy of Sciences of the United States of America.

Ligand-induced conformational changes in a thermophilic ribose-binding protein

H. Hellinga, L. Beese, M. Cuneo, 2008, BMC Structural Biology.

Bmc Structural Biology

Andrei L Lomize, Irina D Pogozheva, Mikhail A. Lomize, 2022 .

Structural reorganization and preorganization in enzyme active sites: comparisons of experimental and theoretically ideal active site geometries in the multistep serine esterase reaction cycle.

H. Hellinga, D. Hilvert, K. Houk, 2008, Journal of the American Chemical Society.

The development of new biotechnologies using metalloprotein design.

H. Hellinga, Hellinga, Benson, 1998, Current opinion in biotechnology.