M. Konrad

发表

Insights into the phosphoryltransfer mechanism of human thymidylate kinase gained from crystal structures of enzyme complexes along the reaction coordinate.

I. Schlichting, N. Ostermann, R. Brundiers, 2000, Structure.

Colloidal micro- and nano-particles as templates for polyelectrolyte multilayer capsules.

M. Konrad, A. Yashchenok, A. Skirtach, 2014, Advances in colloid and interface science.

Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays.

M. Konrad, J. Baret, Jiseok Lim, 2015, Biomicrofluidics.

Ultra-high throughput detection of single cell β-galactosidase activity in droplets using micro-optical lens array.

M. Konrad, J. Baret, Jiseok Lim, 2013, Applied physics letters.

Micro-optical lens array for fluorescence detection in droplet-based microfluidics† †Electronic supplementary information (ESI) available: Supplementary Figures (S1 and S2). Supplementary movie 01: movie recorded by a high-speed camera without backlight illumination. Supplementary movie 02: movie re

Jiseok Lim, Jean-Christophe Baret, Philipp Gruner, 2013, Lab on a chip.

Photo-electrochemical Bioanalysis of Guanosine Monophosphate Using Coupled Enzymatic Reactions at a CdS/ZnS Quantum Dot Electrode.

Marc Riedel, Wolfgang J Parak, Pablo del Pino, 2015, Small.

Bacterial co-expression of the α and β protomers of human l-asparaginase-3: Achieving essential N-terminal exposure of a catalytically critical threonine located in the β-subunit.

M. Konrad, C. S. Karamitros, C. Karamitros, 2014, Protein expression and purification.

Structures of apo and product-bound human L-asparaginase: insights into the mechanism of autoproteolysis and substrate hydrolysis.

M. Konrad, A. Lavie, Ying Su, 2012, Biochemistry.

From Compartmentalization of Bacteria within Inorganic Macrocellular Beads to the Assembly of Microbial Consortia

M. Konrad, J. Baret, V. Schmitt, 2018, Advanced Biosystems.

Functional analysis of the guanylate kinase-like domain in the synapse-associated protein SAP97.

C. Garner, M. Konrad, S. Kuhlendahl, 1998, European journal of biochemistry.

Tropomyosin Exon 6b Is Troponin-specific and Required for Correct Acto-myosin Regulation*

M. Konrad, M. Geeves, R. Maytum, 2004, Journal of Biological Chemistry.

An Amplex Red-based fluorometric and spectrophotometric assay for L-asparaginase using its natural substrate.

M. Konrad, Jiseok Lim, C. S. Karamitros, 2014, Analytical biochemistry.

Cloning and expression of the essential gene for guanylate kinase from yeast.

M. Konrad, Manfred Konrad, 1992, The Journal of biological chemistry.

Analysis and in vivo disruption of the gene coding for adenylate kinase (ADK1) in the yeast Saccharomyces cerevisiae.

M. Konrad, Manfred Konrad, 1988, The Journal of biological chemistry.

Phosphorylation of Human Choline Kinase Beta by Protein Kinase A: Its Impact on Activity and Inhibition

M. Konrad, L. Few, W. C. See Too, 2016, PloS one.

Long-term effect of cyclic AMP on N-glycosylation is caused by an increase in the activity of the cis-prenyltransferase.

M. Konrad, W. Merz, 1996, Biochemical Journal.

Solution structure and functional investigation of human guanylate kinase reveals allosteric networking and a crucial role for the enzyme in cancer

M. Konrad, J. Trent, Pablo Trigo-Mourino, 2019, The Journal of Biological Chemistry.

Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases.

C. Monnerjahn, M. Konrad, A. Lavie, 2003, Structure.

Allosterically regulated human L-asparaginase

M. Konrad, C. S. Karamitros, C. Karamitros, 2014 .

YLR209c Encodes Saccharomyces cerevisiae Purine Nucleoside Phosphorylase

B. Daignan-Fornier, M. Konrad, C. Desgranges, 2001, Journal of bacteriology.

YLR 209 c Encodes Saccharomyces cerevisiae Purine Nucleoside Phosphorylase

B. Daignan-Fornier, M. Konrad, C. Desgranges, 2001 .

Diffusion and drift studies of Ar-DME/CO2/CH4 gas mixtures for a radial TPC in the E > B field

M. Konrad, J. Draper, V. Eckardt, 1997 .

Inactivation of purified Escherichia coli RNA polymerase by transfer RNA.

M. Konrad, H. Bremer, C. Yegian, 1966, Journal of molecular biology.

Preserving catalytic activity and enhancing biochemical stability of the therapeutic enzyme asparaginase by biocompatible multilayered polyelectrolyte microcapsules.

M. Konrad, H. Möhwald, A. Yashchenok, 2013, Biomacromolecules.

Kinetic and thermodynamic properties of the ternary complex between F-actin, myosin subfragment 1 and adenosine 5'-[beta, gamma-imido]triphosphate.

M. Konrad, R. Goody, 1982, European Journal of Biochemistry.

Kinetics of Phosphate Incorporation into Adenosine Triphosphate and Guanosine Triphosphate in Bacteria

M. Konrad, J. Lutkenhaus, J. Ryan, 1973, Journal of Bacteriology.

Alkaline Phosphatase Delivery System Based on Calcium Carbonate Carriers for Acceleration of Ossification.

M. Konrad, V. Atkin, A. Abalymov, 2020, ACS applied bio materials.

Capacity of T4 DNA to serve as template for purified Escerichia coli RNA polymerase.

M. Konrad, H. Bremer, R. Bruner, 1966, Journal of molecular biology.

Controlled enzyme-catalyzed degradation of polymeric capsules templated on CaCO₃: influence of the number of LbL layers, conditions of degradation, and disassembly of multicompartments.

M. Konrad, H. Möhwald, A. Yashchenok, 2012, Journal of controlled release : official journal of the Controlled Release Society.

Loading Capacity versus Enzyme Activity in Anisotropic and Spherical Calcium Carbonate Microparticles.

M. Konrad, H. Möhwald, A. Yashchenok, 2016, ACS applied materials & interfaces.

Temperature Window for Encapsulation of an Enzyme into Thermally Shrunk, CaCO3 Templated Polyelectrolyte Multilayer Capsules.

M. Konrad, Louis Van der Meeren, A. Skirtach, 2020, Macromolecular bioscience.

The sugar ring of the nucleoside is required for productive substrate positioning in the active site of human deoxycytidine kinase (dCK): implications for the development of dCK-activated acyclic guanine analogues.

M. Konrad, A. Lavie, Saugata Hazra, 2010, Journal of medicinal chemistry.

Regulation of N-glycosylation. Long term effect of cyclic AMP mediates enhanced synthesis of the dolichol pyrophosphate core oligosaccharide.

M. Konrad, W. Merz, 1994, The Journal of biological chemistry.

Thermal Stability of Hexameric and Tetrameric Nucleoside Diphosphate Kinases

J. Janin, M. Konrad, I. Lascu, 1996, The Journal of Biological Chemistry.

Structures of human thymidylate kinase in complex with prodrugs: implications for the structure-based design of novel compounds.

C. Meier, C. Monnerjahn, N. Ostermann, 2003, Biochemistry.

Nonenantioselectivity property of human deoxycytidine kinase explained by structures of the enzyme in complex with L- and D-nucleosides.

M. Konrad, A. Lavie, E. Sabini, 2007, Journal of medicinal chemistry.

Structural basis for activation of the therapeutic l-nucleoside analogs 3TC and troxacitabine by human deoxycytidine kinase

S. Burley, M. Konrad, A. Lavie, 2006, Nucleic acids research.

Structural and kinetic characterization of human deoxycytidine kinase variants able to phosphorylate 5-substituted deoxycytidine and thymidine analogues .

Saugata Hazra, M. Konrad, A. Lavie, 2010, Biochemistry.

Extending thymidine kinase activity to the catalytic repertoire of human deoxycytidine kinase.

M. Konrad, A. Lavie, E. Sabini, 2009, Biochemistry.

Mimicking phosphorylation of Ser‐74 on human deoxycytidine kinase selectively increases catalytic activity for dC and dC analogues

M. Konrad, A. Lavie, T. McSorley, 2008, FEBS letters.

Engineered human tmpk/AZT as a novel enzyme/prodrug axis for suicide gene therapy.

M. Konrad, A. Lavie, J. Medin, 2007, Molecular therapy : the journal of the American Society of Gene Therapy.

Substrate-induced Conformational Changes in Human UMP/CMP Kinase*

M. Konrad, A. Lavie, D. Segura-Peña, 2004, Journal of Biological Chemistry.

Post-translational phosphorylation of serine 74 of human deoxycytidine kinase favors the enzyme adopting the open conformation making it competent for nucleoside binding and release.

M. Konrad, A. Lavie, A. Szewczak, 2011, Biochemistry.

Elucidation of different binding modes of purine nucleosides to human deoxycytidine kinase.

M. Konrad, A. Lavie, E. Sabini, 2008, Journal of medicinal chemistry.

Structure of human dCK suggests strategies to improve anticancer and antiviral therapy

C. Monnerjahn, M. Konrad, A. Lavie, 2003, Nature Structural Biology.

Structural basis for substrate promiscuity of dCK.

M. Konrad, A. Lavie, E. Sabini, 2008, Journal of molecular biology.

Modifying Human Thymidylate Kinase to Potentiate Azidothymidine Activation*

I. Schlichting, N. Ostermann, R. Brundiers, 1999, The Journal of Biological Chemistry.

Binding of ATP to TK1-like enzymes is associated with a conformational change in the quaternary structure.

C. Monnerjahn, M. Konrad, A. Lavie, 2007, Journal of molecular biology.

Quaternary structure change as a mechanism for the regulation of thymidine kinase 1-like enzymes.

M. Konrad, A. Lavie, D. Segura-Peña, 2007, Structure.

Structural basis for the preference of UTP over ATP in human deoxycytidine kinase: illuminating the role of main-chain reorganization.

M. Konrad, A. Lavie, E. Sabini, 2006, Biochemistry.

Substrate Specificity of Human Nucleoside-diphosphate Kinase Revealed by Transient Kinetic Analysis*

M. Konrad, M. Geeves, Sabine Schaertl, 1998, The Journal of Biological Chemistry.

Crystal structure of yeast thymidylate kinase complexed with the bisubstrate inhibitor P1-(5'-adenosyl) P5-(5'-thymidyl) pentaphosphate (TP5A) at 2.0 A resolution: implications for catalysis and AZT activation.

I. Schlichting, R. Brundiers, M. Konrad, 1998, Biochemistry.

Structure of thymidylate kinase reveals the cause behind the limiting step in AZT activation

Ilme Schlichting, Roger S. Goody, I. Schlichting, 1997, Nature Structural Biology.

Structural Basis for Nucleotide-dependent Regulation of Membrane-associated Guanylate Kinase-like Domains*

M. Konrad, A. Lavie, O. Spangenberg, 2002, The Journal of Biological Chemistry.

The interaction of bovine pancreatic deoxyribonuclease I and skeletal muscle actin.

M. Konrad, R. Goody, H. Mannherz, 1980, European journal of biochemistry.

Balance of human choline kinase isoforms is critical for cell cycle regulation

M. Konrad, A. Lavie, Jens Gruber, 2012, The FEBS journal.

Free glycine accelerates the autoproteolytic activation of human asparaginase.

M. Konrad, A. Lavie, Ying Su, 2013, Chemistry & biology.

Kinetic and Thermodynamic Properties of the Ternary Complex between F-actin, Myosin Subfragment 1 and Adenosine 5′-[β,γ-imido]triphosphate

M. Konrad, R. Goody, 2005 .

Structural basis for efficient phosphorylation of 3'-azidothymidine monophosphate by Escherichia coli thymidylate kinase.

I. Schlichting, N. Ostermann, R. Brundiers, 1998, Proceedings of the National Academy of Sciences of the United States of America.

Elucidation of human choline kinase crystal structures in complex with the products ADP or phosphocholine.

M. Konrad, A. Lavie, E. Malito, 2006, Journal of Molecular Biology.

Human 60-kDa Lysophospholipase Contains an N-terminal l-Asparaginase Domain That Is Allosterically Regulated by l-Asparagine*

M. Konrad, C. Karamitros, 2014, The Journal of Biological Chemistry.

- Asparagine Domain That Is Allosterically Regulated by Contains an N-terminal l-Asparaginase Human 60-kDa Lysophospholipase Enzymology :

M. Konrad, C. Karamitros, 2014 .

Structural and catalytic properties and homology modelling of the human nucleoside diphosphate kinase C, product of the DRnm23 gene.

J. Cherfils, M. Konrad, I. Lascu, 2001, European journal of biochemistry.

Cloning of the guanylate kinase homologues AGK-1 and AGK-2 from Arabidopsis thaliana and characterization of AGK-1.

M. Konrad, O. Spangenberg, V. Kumar, 2000, European journal of biochemistry.

Internal motions in myosin.

O. Jardetzky, M. Konrad, R. Goody, 1979, Biochemistry.

Ultra Short Yeast Tropomyosins Show Novel Myosin Regulation*

M. Konrad, W. Lehman, M. Geeves, 2008, Journal of Biological Chemistry.

Potentiating AZT activation: structures of wild-type and mutant human thymidylate kinase suggest reasons for the mutants' improved kinetics with the HIV prodrug metabolite AZTMP.

I. Schlichting, N. Ostermann, R. Brundiers, 2000, Journal of molecular biology.

Structural Mechanism for Substrate Inhibition of the Adenosine 5′-Phosphosulfate Kinase Domain of Human 3′-Phosphoadenosine 5′-Phosphosulfate Synthetase 1 and Its Ramifications for Enzyme Regulation*

M. Konrad, A. Lavie, N. Sekulić, 2007, Journal of Biological Chemistry.

Elucidation of the active conformation of the APS-kinase domain of human PAPS synthetase 1.

M. Konrad, A. Lavie, N. Sekulić, 2007, Journal of molecular biology.

Novel 4‐Amino Bis‐pyridinium and Bis‐quinolinium Derivatives as Choline Kinase Inhibitors with Antiproliferative Activity against the Human Breast Cancer SKBR‐3 Cell Line

M. Konrad, J. Campos, T. McSorley, 2012, ChemMedChem.

Yeast GMP kinase mutants constitutively express AMP biosynthesis genes by phenocopying a hypoxanthine-guanine phosphoribosyltransferase defect.

B. Daignan-Fornier, M. Konrad, K. Lecoq, 2000, Genetics.

Metabolomics and proteomics identify the toxic form and the associated cellular binding targets of the anti-proliferative drug AICAR

B. Daignan-Fornier, M. Konrad, S. Claverol, 2018, The Journal of Biological Chemistry.

Restoration of the antiviral activity of 3'-azido-3'-deoxythymidine (AZT) against AZT-resistant human immunodeficiency virus by delivery of engineered thymidylate kinase to T cells.

C. Monnerjahn, M. Konrad, A. Lavie, 2008, The Journal of general virology.

N-terminally acetylated tropomyosin generated in E. coli by coexpression of the S. cerevisiae NatB acetylation complex shows functional properties in vitro

M. Konrad, R. Maytum, 2006 .

A Point Mutation of Human Nucleoside Diphosphate Kinase A Found in Aggressive Neuroblastoma Affects Protein Folding*

M. Konrad, I. Lascu, M. Lacombe, 1997, The Journal of Biological Chemistry.

Binding of Nucleotides to Guanylate Kinase, p21 ras , and Nucleoside-diphosphate Kinase Studied by Nano-electrospray Mass Spectrometry*

M. Konrad, A. Lavie, H. Prinz, 1999, The Journal of Biological Chemistry.

Melting transitions in biomembranes.

M. Konrad, T. Heimburg, T. Mužić, 2019, Biochimica et biophysica acta. Biomembranes.

Enzyme reaction in the pores of CaCO3 particles upon ultrasound disruption of attached substrate-filled liposomes.

M. Konrad, T. Jovin, H. Möhwald, 2010, Angewandte Chemie.

Co‐regulation of yeast purine and phosphate pathways in response to adenylic nucleotide variations

B. Daignan-Fornier, M. Konrad, Sébastien A. Gauthier, 2008, Molecular microbiology.

Highly Specific Antibodies for Co-Detection of Human Choline Kinase α1 and α2 Isoforms

M. Konrad, L. Few, W. C. See Too, 2010, PloS one.

Insights into open/closed conformations of the catalytically active human guanylate kinase as investigated by small-angle X-ray scattering

A. Menzel, S. Techert, M. Konrad, 2015, European Biophysics Journal.

Formation of Complexes between Ca2+·Calmodulin and the Synapse-associated Protein SAP97 Requires the SH3 Domain-Guanylate Kinase Domain-connecting HOOK Region*

M. Konrad, A. Lavie, Manfred Konrad, 2002, The Journal of Biological Chemistry.

Molecular analysis of the essential gene for adenylate kinase from the fission yeast Schizosaccharomyces pombe.

M. Konrad, 1993, The Journal of biological chemistry.

Structural Characterization of the Closed Conformation of Mouse Guanylate Kinase*

M. Konrad, A. Lavie, N. Sekulić, 2002, The Journal of Biological Chemistry.

1H, 13C and 15N resonance assignment of human guanylate kinase

M. Konrad, Pablo Trigo-Mourino, Donghan Lee, 2018, Biomolecular NMR assignments.

1H, 13C and 15N resonance assignment of human guanylate kinase

M. Konrad, Pablo Trigo-Mourino, Donghan Lee, 2017, Biomolecular NMR Assignments.

Human Nucleoside Diphosphate Kinase B (Nm23-H2) from Melanoma Cells Shows Altered Phosphoryl Transfer Activity Due to the S122P Mutation*

M. Konrad, M. Geeves, S. Schaertl, 1999, The Journal of Biological Chemistry.

Modulated Nucleoside Kinases as Tools to Improve the Activation of Therapeutic Nucleoside Analogues

C. Monnerjahn, M. Konrad, 2003, Chembiochem : a European journal of chemical biology.

Identification and characterization of a yeast gene encoding an adenylate kinase homolog.

M. Konrad, 1993, Biochimica et biophysica acta.

Complexes of yeast adenylate kinase and nucleotides investigated by proton NMR

M. Konrad, I. Vetter, P. Rösch, 1991 .

Improving AZT efficacy

I. Schlichting, M. Konrad, J. Reinstein, 1998 .

Reply to “Improving AZT efficacy”

I. Schlichting, M. Konrad, J. Reinstein, 1998, Nature Medicine.

Fluorescence-Activated Cell Sorting of Human l-asparaginase Mutant Libraries for Detecting Enzyme Variants with Enhanced Activity.

M. Konrad, C. Karamitros, 2016, ACS chemical biology.

On the structure of the myosin‐ADP‐Mg complex

M. Konrad, R. Goody, W. Hofman, 1981, FEBS letters.

Liposomes for microcompartmentation of enzymes and their influence on catalytic activity.

M. Konrad, M. Hoppert, F. Mayer, 2003, Biochemical and biophysical research communications.

Regulatory properties of tropomyosin effects of length, isoform, and N-terminal sequence.

M. Konrad, M. Geeves, R. Maytum, 2001, Biochemistry.

Actomyosin regulatory properties of yeast tropomyosin are dependent upon N-terminal modification.

M. Konrad, M. Geeves, R. Maytum, 2000, Biochemistry.

Structural requirements for efficient phosphorylation of nucleotide analogs by human thymidylate kinase.

M. Konrad, A. Lavie, 2004, Mini reviews in medicinal chemistry.

Expressing engineered thymidylate kinase variants in human cells to improve AZT phosphorylation and human immunodeficiency virus inhibition.

R. Brundiers, M. Konrad, R. Goody, 2005, The Journal of general virology.

A designed equine herpes thymidine kinase (EHV4 TK) variant improves ganciclovir-induced cell-killing.

C. Monnerjahn, M. Konrad, T. McSorley, 2014, Biochemical pharmacology.

Structural requirements for calmodulin binding to membrane‐associated guanylate kinase homologs

M. Konrad, A. Lavie, I. Paarmann, 2008, Protein science : a publication of the Protein Society.

Selective spin diffusion. A novel method for studying motional properties of biopolymers in solution

M. Konrad, R. Goody, K. Akasaka, 1978, FEBS letters.

Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays.

M. Konrad, J. Baret, Jiseok Lim, 2015, Biomicrofluidics.

Loading Capacity versus Enzyme Activity in Anisotropic and Spherical Calcium Carbonate Microparticles.

M. Konrad, H. Möhwald, A. Yashchenok, 2016, ACS applied materials & interfaces.

Melting transitions in biomembranes.

M. Konrad, T. Heimburg, Søren B Madsen, 2019, Biochimica et biophysica acta. Biomembranes.