D. Speicher

发表

Disulfide Bond Assignments of Secreted Frizzled-related Protein-1 Provide Insights about Frizzled Homology and Netrin Modules*

J. M. Chong, A. Üren, J. Rubin, 2002, The Journal of Biological Chemistry.

HUPO Plasma Proteome Project specimen collection and handling: Towards the standardization of parameters for plasma proteome samples

Graham B. I. Scott, G. Siest, D. Chan, 2005, Proteomics.

A novel four‐dimensional strategy combining protein and peptide separation methods enables detection of low‐abundance proteins in human plasma and serum proteomes

D. Speicher, J. Rux, N. Levenkova, 2005, Proteomics.

Systematic comparison of fractionation methods for in-depth analysis of plasma proteomes.

D. Speicher, Hsin-Yao Tang, Z. Cao, 2012, Journal of proteome research.

Challenges in deriving high-confidence protein identifications from data gathered by a HUPO plasma proteome collaborative study

S. Hanash, D. States, G. Omenn, 2006, Nature Biotechnology.

Uncovering thousands of new HLA antigens and phosphopeptides with deep learning-based sequence-mask-search de novo peptide sequencing framework

E. Chuangsuwanich, D. Speicher, S. Sriswasdi, 2019, bioRxiv.

Overcoming intrinsic multidrug resistance in melanoma by blocking the mitochondrial respiratory chain of slow-cycling JARID1B(high) cells.

P. Gimotty, M. Herlyn, D. Speicher, 2013, Cancer cell.

Deciphering the human platelet sheddome.

T. Grosser, D. Speicher, I. Blair, 2011, Blood.

Erythrocyte spectrin is comprised of many homologous triple helical segments

Vincent T. Marchesi, David W. Speicher, D. Speicher, 1984, Nature.

TEMPERATURE-COUPLED FORCED UNFOLDING OF SINGLE PROTEINS

D. Speicher, D. Discher, R. Law, 2022 .

Systematic analysis of peptide recoveries from in-gel digestions for protein identifications in proteome studies.

D. Speicher, K. Speicher, O. Kolbas, 2000, Journal of biomolecular techniques : JBT.

Nuclear Lamin-A Scales with Tissue Stiffness and Enhances Matrix-Directed Differentiation

Dennis E. Discher, Jae-Won Shin, Florian Rehfeldt, 2013, Science.

Embryonic cardiomyocytes beat best on a matrix with heart-like elasticity: scar-like rigidity inhibits beating

Adam J Engler, Dennis E Discher, David W Speicher, 2008, Journal of Cell Science.

Cyclophilin: a specific cytosolic binding protein for cyclosporin A.

D. Speicher, J. Rice, M. Harding, 1984, Science.

Gene expression profiles in peripheral blood mononuclear cells can distinguish patients with non-small cell lung cancer from patients with nonmalignant lung disease.

Malik Yousef, Anil Vachani, Andrew V Kossenkov, 2009, Cancer research.

Molecular analysis of insertion/deletion mutations in protein 4.1 in elliptocytosis. I. Biochemical identification of rearrangements in the spectrin/actin binding domain and functional characterizations.

J. Conboy, D. Speicher, P. Agre, 1990, The Journal of clinical investigation.

Analysis of human red cell spectrin tetramer (head-to-head) assembly using complementary univalent peptides.

D. Speicher, K. Peng, T. DeSilva, 1992, Biochemistry.

Location of the human red cell spectrin tetramer binding site and detection of a related "closed" hairpin loop dimer using proteolytic footprinting.

D. Speicher, T. DeSilva, K. Speicher, 1993, The Journal of biological chemistry.

Mapping the Human Erythrocyte -Spectrin Dimer Initiation Site Using Recombinant Peptides and Correlation of Its Phasing with the -Actinin Dimer Site (*)

D. Speicher, T. DeSilva, P. Curtis, 1996, Journal of Biological Chemistry.

Mapping the human erythrocyte beta-spectrin dimer initiation site using recombinant peptides and correlation of its phasing with the alpha-actinin dimer site.

D. Speicher, T. DeSilva, P. Curtis, 1996, The Journal of biological chemistry.

Identification of a long non‐coding RNA‐associated RNP complex regulating metastasis at the translational step

Kiranmai Gumireddy, Julia Tchou, G. Calin, 2013, The EMBO journal.

N‐Terminal Sequence Analysis of Proteins and Peptides

D. Speicher, K. Speicher, Nicole R. Gorman, 2009, Current protocols in protein science.

N‐Terminal Sequence Analysis of Proteins and Peptides

D. Speicher, D. Reim, 1997, Current protocols in protein science.

Endoplasmic Reticulum PI(3)P Lipid Binding Targets Malaria Proteins to the Host Cell

K. Haldar, D. Speicher, R. Stahelin, 2012, Cell.

Myc-mediated transcriptional regulation of the mitochondrial chaperone TRAP1 controls primary and metastatic tumor growth

C. Dang, D. Speicher, J. C. Ghosh, 2019, The Journal of Biological Chemistry.

Purification and characterization of double-stranded RNA adenosine deaminase from bovine nuclear extracts.

D. Speicher, K. Nishikura, J. Murray, 1994, The Journal of biological chemistry.

The Motor Protein Kinesin-1 Links Neurofibromin and Merlin in a Common Cellular Pathway of Neurofibromatosis*

R. Shiekhattar, D. Speicher, M. Hakimi, 2002, The Journal of Biological Chemistry.

Effects of plating density and age in culture on growth and cell division of neonatal rat heart primary cultures

D. Speicher, R. McCarl, J. N. Peace, 1981, In Vitro.

Crystal structure and functional interpretation of the erythrocyte spectrin tetramerization domain complex.

A. Mondragón, D. Speicher, R. Marmorstein, 2010, Blood.

Identification of functional domains of human erythrocyte spectrin.

D. Speicher, V. Marchesi, J. Morrow, 1980, Proceedings of the National Academy of Sciences of the United States of America.

Identification of proteolytically resistant domains of human erythrocyte spectrin.

D. Speicher, V. Marchesi, J. Morrow, 1980, Proceedings of the National Academy of Sciences of the United States of America.

Molecular and functional changes in spectrin from patients with hereditary pyropoikilocytosis.

D. Speicher, V. Marchesi, N. Mohandas, 1983, The Journal of clinical investigation.

sFRP2 in the aged microenvironment drives melanoma metastasis and therapy resistance

L. Ferrucci, D. Schadendorf, B. Schilling, 2016, Nature.

Probing structures of large protein complexes using zero-length cross-linking.

D. Speicher, S. Sriswasdi, S. Harper, 2015, Methods.

IL8 and Cathepsin B as Melanoma Serum Biomarkers

D. Speicher, Xiaowei Xu, L. Schuchter, 2011, International journal of molecular sciences.

PPT1 Promotes Tumor Growth and Is the Molecular Target of Chloroquine Derivatives in Cancer.

P. Gimotty, M. Herlyn, Gao Zhang, 2018, Cancer discovery.

A Unified Approach to Targeting the Lysosome's Degradative and Growth Signaling Roles.

G. Mills, M. Herlyn, Yiling Lu, 2017, Cancer discovery.

Melanoma cell-cell interactions are mediated through heterophilic Mel-CAM/ligand adhesion.

I. Shih, D. Speicher, M. Herlyn, 1997, Cancer research.

Activation Nucleosome Acetylation and Transcription Targeting Are Required for Zta-Directed The CBP Bromodomain and Nucleosome

D. Speicher, G. Blobel, P. Lieberman, 2003 .

The CBP Bromodomain and Nucleosome Targeting Are Required for Zta-Directed Nucleosome Acetylation and Transcription Activation

D. Speicher, G. Blobel, P. Lieberman, 2003, Molecular and Cellular Biology.

Aggregatibacter actinomycetemcomitans leukotoxin requires beta-sheets 1 and 2 of the human CD11a beta-propeller for cytotoxicity.

D. Speicher, E. Lally, I. Kieba, 2007, Cellular microbiology.

Aggregatibacter actinomycetemcomitans leukotoxin requires β‐sheets 1 and 2 of the human CD11a β‐propeller for cytotoxicity

D. Speicher, E. Lally, I. Kieba, 2007, Cellular microbiology.

Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice*

E. Puré, D. Speicher, C. Feghali-Bostwick, 2015, The Journal of Biological Chemistry.

Cdc13 OB2 dimerization required for productive Stn1 binding and efficient telomere maintenance.

E. Skordalakes, F. Johnson, D. Speicher, 2013, Structure.

MYST protein acetyltransferase activity requires active site lysine autoacetylation

Chao Yang, S. Berger, R. Sternglanz, 2012, The EMBO journal.

Changes in Aged Fibroblast Lipid Metabolism Induce Age-dependent Melanoma Cell Resistance to Targeted Therapy Via the Fatty Acid Transporter FATP2.

Stephen M. Douglass, A. Weeraratna, K. Flaherty, 2020, Cancer discovery.

Protein Posttranslational Signatures Identified in COVID-19 Patient Plasma

D. Speicher, A. Kashina, Hsin-Yao Tang, 2021, bioRxiv.

In-depth analysis of a plasma or serum proteome using a 4D protein profiling method.

D. Speicher, Hsin-Yao Tang, Lynn A. Beer, 2011, Methods in molecular biology.

High Sensitivity Peptide Sequence Analysis Using In Situ Proteolysis on High Retention PVDF Membranes and a Biphasic Reaction Column Sequencer

D. Speicher, D. Reim, Sandra Best, 1994 .

Human PC4 Is a Substrate-specific Inhibitor of RNA Polymerase II Phosphorylation*

A. Shilatifard, R. Shiekhattar, D. Speicher, 2000, The Journal of Biological Chemistry.

Molecular diagnosis of ectopic pregnancy

K. Barnhart, D. Speicher, 2002, Expert review of molecular diagnostics.

A disintegrin and metalloprotease protein-12 as a novel marker for the diagnosis of ectopic pregnancy.

K. Barnhart, A. Shaunik, D. Speicher, 2011, Fertility and sterility.

Mitogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A(2) activity.

D. Speicher, C. Dodia, A. Fisher, 2009, The Biochemical journal.

Mass spectrometric and computational analysis of cytokine‐induced alterations in the astrocyte secretome

R. Balice-Gordon, D. Speicher, T. Greco, 2009, Proteomics.

Immunoaffinity Depletion of High Abundance Plasma and Serum Proteins

D. Speicher, L. Echan, 2009 .

Enhanced identification of zero-length chemical cross-links using label-free quantitation and high-resolution fragment ion spectra.

D. Speicher, S. Sriswasdi, Hsin-Yao Tang, 2014, Journal of proteome research.

Protein Detection in Gels Using Fixation

D. Speicher, Lynn A. Beer, 2018, Current protocols in protein science.

Protein Detection in Gels Using Fixation

D. Speicher, L. Echan, 2002, Current protocols in protein science.

Uncovering Thousands of New Peptides with Sequence-Mask-Search Hybrid De Novo Peptide Sequencing Framework*

E. Chuangsuwanich, D. Speicher, S. Sriswasdi, 2019, Molecular & Cellular Proteomics.

Structural basis for dimerization in DNA recognition by Gal4.

D. Speicher, R. Marmorstein, M. X. Fitzgerald, 2008, Structure.

High-sensitivity gas phase sequence analysis of proteins and peptides on PVDF membranes using short cycle times.

D. Speicher, D. Reim, 1993, Analytical biochemistry.

Microsequence analysis of electroblotted proteins. II. Comparison of sequence performance on different types of PVDF membranes.

D. Speicher, D. Reim, 1992, Analytical biochemistry.

Microsequence analysis of electroblotted proteins. I. Comparison of electroblotting recoveries using different types of PVDF membranes.

D. Speicher, J. Mozdzanowski, 1992, Analytical biochemistry.

Depletion of multiple high‐abundance proteins improves protein profiling capacities of human serum and plasma

D. Speicher, Hsin-Yao Tang, N. Ali-Khan, 2005, Proteomics.

Enhanced analysis of human breast cancer proteomes using micro-scale solution isoelectrofocusing combined with high resolution 1-D and 2-D gels.

D. Speicher, X. Zuo, L. Echan, 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

HRS phosphorylation drives immunosuppressive exosome secretion and restricts CD8+ T-cell infiltration into tumors

K. Flaherty, Dennie T. Frederick, T. Svitkina, 2022, Nature Communications.

Probing erectile function: S-(2-boronoethyl)-L-cysteine binds to arginase as a transition state analogue and enhances smooth muscle relaxation in human penile corpus cavernosum.

D. Speicher, D. Christianson, N. Kim, 2001, Biochemistry.

Blood Deciphering the human platelet sheddome

T. Grosser, D. Speicher, I. Blair, 2010 .

Purification of proteins fused to glutathione S-transferase.

D. Speicher, S. Harper, 2011, Methods in molecular biology.

Aggregatibacter actinomycetemcomitans leukotoxin is post-translationally modified by addition of either saturated or hydroxylated fatty acyl chains.

D. Speicher, E. Lally, I. Kieba, 2011, Molecular oral microbiology.

Mutant forms of spectrin alpha-subunits in hereditary elliptocytosis.

D. Speicher, P. Agre, V. Marchesi, 1987, The Journal of clinical investigation.

Stromal changes in the aged lung induce an emergence from melanoma dormancy.

Stephen M. Douglass, A. Weeraratna, E. Jaffee, 2022, Nature.

Stromal changes in the aged lung induce an emergence from melanoma dormancy

Stephen M. Douglass, A. Weeraratna, E. Jaffee, 2020, Nature.

Corrigendum: sFRP2 in the aged microenvironment drives melanoma metastasis and therapy resistance

L. Ferrucci, D. Schadendorf, B. Schilling, 2016, Nature.

Peroxynitrite in the tumor microenvironment changes the profile of antigens allowing escape from cancer immunotherapy.

L. Montaner, D. Speicher, C. Meyer, 2022, Cancer cell.

Determination of Disulfide Bond Assignments andN-Glycosylation Sites of the Human Gastrointestinal Carcinoma Antigen GA733-2 (CO17-1A, EGP, KS1-4, KSA, and Ep-CAM)*

J. M. Chong, D. Speicher, 2001, The Journal of Biological Chemistry.

Mammalian Tropomodulins Nucleate Actin Polymerization via Their Actin Monomer Binding and Filament Pointed End-capping Activities*

D. Speicher, V. Fowler, K. Speicher, 2010, The Journal of Biological Chemistry.

Identification of secreted proteins that reflect autophagy dynamics within tumor cells

P. Gimotty, M. Herlyn, Gao Zhang, 2015, Autophagy.

Oxidation of Peroxiredoxin 6 in the Presence of GSH Increases its Phospholipase A2 Activity at Cytoplasmic pH

D. Speicher, H. Forman, C. Dodia, 2018, Antioxidants.

Proteome analysis : interpreting the genome

D. Speicher, 2004 .

Circadian lncRNA ADIRF-AS1 binds PBAF and regulates renal clear cell tumorigenesis.

C. Dang, D. Speicher, A. Kossenkov, 2022, Cell reports.

Identification of the functional site of erythrocyte protein 4.1 involved in spectrin-actin associations.

D. Speicher, V. Marchesi, I. Correas, 1986, The Journal of biological chemistry.

3 – MICROSEQUENCING WITH PVDF MEMBRANES: EFFICIENT ELECTROBLOTTING, DIRECT PROTEIN ADSORPTION AND SEQUENCER PROGRAM MODIFICATIONS1

D. Speicher, 1989 .

Methods and Strategies for the Sequence Analysis of Proteins on PVDF Membranes

D. Speicher, 1994 .

Heterogeneity of Proteolytic Enzyme Preparations Commonly Employed for Dispersal of Solid Tissues

D. Speicher, 1987 .

Timeless Links Replication Termination to Mitotic Kinase Activation

D. Speicher, A. Gotter, P. Lieberman, 2011, PloS one.

Structural domain mapping and phosphorylation of human erythrocyte pallidin (band 4.2).

D. Speicher, B. Guptaroy, C. M. Cohen, 1993, Biochimica et biophysica acta.

Structural and functional features of the alpha-1 domain from human erythrocyte spectrin.

D. Speicher, 1984, Progress in clinical and biological research.

Synthetic lethality by targeting EZH2 methyltransferase activity in ARID1A-mutated cancers

Benjamin G. Bitler, I. Shih, D. Speicher, 2015, Nature Medicine.

Characterizing Recombinant Proteins Using HPLC Gel Filtration and Mass Spectrometry

D. Speicher, G. Begg, S. Harper, 1999, Current protocols in protein science.

Biochemical and functional characterization of aminopeptidase N expressed by human melanoma cells.

D. Speicher, M. Herlyn, A. Menrad, 1993, Cancer research.

8 – Quantitative Electrotransfer of Proteins From Polyacrylamide Gels onto PVDF Membranes

D. Speicher, J. Mozdzanowski, 1990 .

Monoclonal antibodies as probes of domain structure of the spectrin alpha subunit.

D. Speicher, V. Marchesi, J. Morrow, 1982, The Journal of biological chemistry.

PPT1 inhibition enhances the antitumor activity of anti–PD-1 antibody in melanoma

E. Wherry, P. Gimotty, D. Speicher, 2020, JCI insight.

Biochemical and Functional Characterization of Aminopeptidase N Expressed by Human Melanoma Cells 1

M. Herlyn, D. Speicher, A. Menrad, 2006 .

A tight junction-associated Merlin-angiomotin complex mediates Merlin's regulation of mitogenic signaling and tumor suppressive functions.

A. Stemmer-Rachamimov, D. Speicher, J. Kissil, 2011, Cancer cell.

cDNA sequence for human erythrocyte ankyrin.

Y. Kan, D. Speicher, J. Lawler, 1990, Proceedings of the National Academy of Sciences of the United States of America.

Structure of human erythrocyte spectrin. II. The sequence of the alpha-I domain.

D. Speicher, V. Marchesi, G. Davis, 1983, The Journal of biological chemistry.

A structural model of human erythrocyte spectrin. Alignment of chemical and functional domains.

D. Speicher, V. Marchesi, J. Morrow, 1982, The Journal of biological chemistry.

Cells Characterization of Tenascin Secreted by Human Melanoma Updated Version Citing Articles E-mail Alerts Characterization of Tenascin Secreted by Human Melanoma Cells1

D. Guerry, D. Speicher, M. Herlyn, 2022 .

A Novel Inhibitor of HSP70 Induces Mitochondrial Toxicity and Immune Cell Recruitment in Tumors

D. Speicher, M. Murphy, D. George, 2020, Cancer Research.

A Synthetic Peptide for Evaluating Protein Sequencing Capabilities: Design of ABRF-93SEQ and Results

P. Andrews, D. Speicher, J. Rush, 1994 .

Evaluation of Protein Sequencing Core Facilities: Design, Characterization, and Results from a Test Sample (ABRF-91SEQ)

C. Slaughter, D. Speicher, G. A. Grant, 1992 .

Targeting glutamine dependence through GLS1 inhibition suppresses ARID1A-inactivated clear cell ovarian carcinoma

D. Huntsman, T. Fukumoto, D. Speicher, 2021, Nature Cancer.

Amino acid sequence similarity between rabies virus glycoprotein and snake venom curaremimetic neurotoxins.

D. Speicher, E. Hawrot, T. Lentz, 1984, Science.

Overview of the HUPO Plasma Proteome Project: Results from the pilot phase with 35 collaborating laboratories and multiple analytical groups, generating a core dataset of 3020 proteins and a publicly‐available database

Eugene A. Kapp, S. Hanash, R. Aebersold, 2005, Proteomics.

129Xe NMR-Protein Sensor Reveals Cellular Ribose Concentration.

D. Speicher, I. Dmochowski, S. D. Zemerov, 2020, Analytical chemistry.

A new chromophoric assay for arginase activity.

D. Speicher, D. Christianson, S. Harper, 1999, Analytical biochemistry.

Myc Regulation of a Mitochondrial Trafficking Network Mediates Tumor Cell Invasion and Metastasis

C. Dang, D. Speicher, J. C. Ghosh, 2019, Molecular and Cellular Biology.

Identification of alternative products and optimization of 2-nitro-5-thiocyanatobenzoic acid cyanylation and cleavage at cysteine residues.

D. Speicher, Hsin-Yao Tang, 2004, Analytical biochemistry.

The Proteome of the Mouse Photoreceptor Sensory Cilium Complex*S

Edward N Pugh, Natasha Levenkova, David W Speicher, 2007, Molecular & Cellular Proteomics.

Isolation and amino acid sequence of cyclophilin.

D. Speicher, M. Harding, M W Harding, 1986, The Journal of biological chemistry.

Detection of Proteins on Blot Membranes

D. Speicher, S. Harper, A. Goldman, 1995, Current protocols in protein science.

Electroblotting from Polyacrylamide Gels

D. Speicher, J. Ursitti, A. Goldman, 1995, Current protocols in protein science.

15 – Design, Characterization and Results of ABRF-89SEQ: A Test Sample For Evaluating Protein Sequencer Performance in Protein Microchemistry Core Facilities

David W. Speicher, Kenneth R. Williams, Ronald L. Niece, 1990 .

Detection of Proteins on Blot Membranes

D. Speicher, S. Harper, 1995, Current protocols in protein science.

UNIT 10.8 Detection of Proteins on Blot Membranes

D. Speicher, S. Harper, 2001 .

High yield electroblotting onto polyvinylidene difluoride membranes from polyacrylamide gels

D. Speicher, P. Hembach, J. Mozdzanowski, 1992, Electrophoresis.

Pyruvate dehydrogenase inactivation causes glycolytic phenotype in BAP1 mutant uveal melanoma

D. Speicher, A. Aplin, J. Harbour, 2022, Oncogene.

Cancer-Associated Fibroblasts Neutralize the Anti-tumor Effect of CSF1 Receptor Blockade by Inducing PMN-MDSC Infiltration of Tumors.

Chunsheng Zhang, N. Hockstein, A. Weeraratna, 2017, Cancer cell.

Remodeling of the Collagen Matrix in Aging Skin Promotes Melanoma Metastasis and Affects Immune Cell Motility.

Stephen M. Douglass, A. Raj, A. Weeraratna, 2018, Cancer discovery.

CARM1-expressing ovarian cancer depends on the histone methyltransferase EZH2 activity

Benjamin G. Bitler, D. Speicher, R. Drapkin, 2018, Nature Communications.

Mass Spectrometry

D. Speicher, 2011 .

Neural Crest-Like Stem Cell Transcriptome Analysis Identifies LPAR1 in Melanoma Progression and Therapy Resistance

G. Mills, J Zhang, K. Flaherty, 2021, Cancer Research.

Dysferlin and Other Non-Red Cell Proteins Accumulate in the Red Cell Membrane of Diamond-Blackfan Anemia Patients

Esther N. Pesciotta, D. Speicher, S. Sriswasdi, 2014, PloS one.

Isolation of human NURF: a regulator of Engrailed gene expression

R. Shiekhattar, William Arbuthnot Sir Lane, D. Speicher, 2003, The EMBO journal.

A Candidate X-linked Mental Retardation Gene Is a Component of a New Family of Histone Deacetylase-containing Complexes*

R. Shiekhattar, William Arbuthnot Sir Lane, D. Speicher, 2003, The Journal of Biological Chemistry.

MYC regulates fatty acid metabolism through a multigenic program in claudin-low triple negative breast cancer

D. Speicher, E. Gottlieb, P. Adams, 2020, British Journal of Cancer.

MFF REGULATION OF MITOCHONDRIAL CELL DEATH IS A THERAPEUTIC TARGET IN CANCER.

M. Herlyn, E. Skordalakes, D. Speicher, 2019, Cancer research.

Molecular identification of a major palmitoylated erythrocyte membrane protein containing the src homology 3 motif.

D. Speicher, P. Ruff, A. Husain-Chishti, 1991, Proceedings of the National Academy of Sciences of the United States of America.

A chromatin remodelling complex that loads cohesin onto human chromosomes

R. Shiekhattar, D. Speicher, K. Yokomori, 2002, Nature.

Efficient Quantitative Comparisons of Plasma Proteomes Using Label-Free Analysis with MaxQuant.

K. Barnhart, D. Speicher, Pengyuan Liu, 2017, Methods in molecular biology.

Molecular Determinants for Targeting Heterochromatin Protein 1-Mediated Gene Silencing: Direct Chromoshadow Domain–KAP-1 Corepressor Interaction Is Essential

David W. Speicher, D. Speicher, M. Lechner, 2000, Molecular and Cellular Biology.

The Physiological Molecular Shape of Spectrin: A Compact Supercoil Resembling a Chinese Finger Trap

Esther Bullitt, Jeffrey W. Brown, Sira Sriswasdi, 2015, PLoS Comput. Biol..

ARID1A-mutated ovarian cancers depend on HDAC6 activity

Kathleen R. Cho, Benjamin G. Bitler, D. Huntsman, 2017, Nature Cell Biology.

The Primary Effect on the Proteome of ARID1A-mutated Ovarian Clear Cell Carcinoma is Downregulation of the Mevalonate Pathway at the Post-transcriptional Level*

Benjamin G. Bitler, D. Speicher, Rugang Zhang, 2016, Molecular & Cellular Proteomics.

Targeting EZH2 methyltransferase activity in ARID1A mutated cancer cells is synthetic lethal

I. Shih, D. Speicher, Hua Li, 2015, Nature medicine.

KAP-1, a novel corepressor for the highly conserved KRAB repression domain.

D. Speicher, E. Neilson, J. Friedman, 1996, Genes & development.

Sequence investigation of the major gastrointestinal tumor-associated antigen gene family, GA733.

D. Speicher, A. Ross, H. Koprowski, 1989, Proceedings of the National Academy of Sciences of the United States of America.

CLIC1 and CLIC4 complement CA125 as a diagnostic biomarker panel for all subtypes of epithelial ovarian cancer

Benjamin G. Bitler, D. Speicher, R. Drapkin, 2018, Scientific Reports.

CLIC1 and CLIC4 complement CA125 as a diagnostic biomarker panel for all subtypes of epithelial ovarian cancer

Benjamin G. Bitler, D. Speicher, R. Drapkin, 2018, Scientific Reports.

A xenograft mouse model coupled with in-depth plasma proteome analysis facilitates identification of novel serum biomarkers for human ovarian cancer.

P. Gimotty, G. Coukos, D. Speicher, 2012, Journal of proteome research.

Measuring MYC-Mediated Metabolism in Tumorigenesis.

C. Dang, D. Speicher, Hsin-Yao Tang, 2021, Methods in molecular biology.

BET Bromodomain Inhibition Synergizes with PARP Inhibitor in Epithelial Ovarian Cancer.

Andrew J. Wilson, Andrew V Kossenkov, Hengrui Zhu, 2017, Cell reports.

Aberrant splicing contributes to severe α-spectrin-linked congenital hemolytic anemia.

P. Newburger, D. Speicher, S. Israels, 2019, The Journal of clinical investigation.

Aberrant splicing contributes to severe alpha-spectrin-linked congenital hemolytic anemia

P. Newburger, D. Speicher, S. Israels, 2022 .

Epigenetic regulation of condensin-mediated genome organization during the cell cycle and upon DNA damage through histone H3 lysine 56 acetylation.

A. G. Chatterjee, D. Speicher, Hideki Tanizawa, 2012, Molecular cell.

ADAR1 controls apoptosis of stressed cells by inhibiting Staufen1-mediated mRNA decay

E. Skordalakes, D. Speicher, M. Sakurai, 2017, Nature Structural &Molecular Biology.

Stromal cyclin D 1 promotes heterotypic immune signaling and breast cancer growth

Lisanti, Amy R. Peck, H. Rui, 2020 .

Stromal cyclin D1 promotes heterotypic immune signaling and breast cancer growth

Amy R. Peck, H. Rui, D. Speicher, 2017, Oncotarget.

NAD+ metabolism governs the proinflammatory senescence-associated secretome

T. Fukumoto, D. Speicher, Rugang Zhang, 2019, Nature Cell Biology.

Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence.

D. Speicher, Rugang Zhang, Linh Le, 2017, Methods in molecular biology.

Comprehensive analysis of the ubiquitinome during oncogene-induced senescence in human fibroblasts

D. Speicher, Rugang Zhang, Hsin-Yao Tang, 2015, Cell cycle.

Reconstitution of the KRAB-KAP-1 repressor complex: a model system for defining the molecular anatomy of RING-B box-coiled-coil domain-mediated protein-protein interactions.

D. Speicher, J. Friedman, D. C. Schultz, 2000, Journal of molecular biology.

Isolation and functional characterization of the A32 melanoma-associated antigen.

D. Elder, I. Shih, M. Herlyn, 1994, Cancer research.

Pancreatic enzyme requirements for the dissociation of rat hearts for culture

D. Speicher, R. McCarl, R. L. McCarl, 1974, In Vitro.

Identification of the amino acid mutations associated with human erythrocyte spectrin alpha II domain polymorphisms.

D. Speicher, K. Speicher, B. DiPaolo, 1993, Blood.

Amino‐acid substitution in α‐spectrin commonly coinherited with nondominant hereditary spherocytosis

D. Speicher, P. Agre, B. Forget, 1997, American journal of hematology.

Selective abrogation of S6K2 maps lipid homeostasis as a survival vulnerability in MAPKi-resistant NRASMUT melanoma

G. Mills, Yiling Lu, D. Speicher, 2021, bioRxiv.

Cancer from Patients with Nonmalignant Lung Disease Small Cell Lung − Cells Can Distinguish Patients with Non Gene Expression Profiles in Peripheral Blood Mononuclear

W. Rom, D. Speicher, M. Yousef, 2009 .

Overview of Proteome Analysis

D. Speicher, X. Zuo, N. Ali-Khan, 2002, Current protocols in protein science.

Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence

D. Speicher, Rugang Zhang, K. Aird, 2020, Nature Communications.

Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence

T. Fukumoto, D. Speicher, Rugang Zhang, 2020, Nature Communications.

Serial Cervicovaginal Exposures With Replication-Deficient SIVsm Induce Higher Dendritic Cell (pDC) and CD4+ T-Cell Infiltrates Not Associated With Prevention but a More Severe SIVmac251 Infection of Rhesus Macaques

L. Montaner, A. Haase, D. Speicher, 2014, Journal of acquired immune deficiency syndromes.

Comparative Secretome Analysis of Epithelial and Mesenchymal Subpopulations of Head and Neck Squamous Cell Carcinoma Identifies S100A4 as a Potential Therapeutic Target*

M. Herlyn, Gao Zhang, D. Speicher, 2013, Molecular & Cellular Proteomics.

Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration.

D. Speicher, J. Lambris, J. Harnly, 2014, Human molecular genetics.

Expression and purification of GST fusion proteins.

D. Speicher, S. Harper, 1997, Current protocols in protein science.

Ghost mitochondria drive metastasis through adaptive GCN2/Akt therapeutic vulnerability

D. Speicher, J. C. Ghosh, V. Vaira, 2022, Proceedings of the National Academy of Sciences.

Molecular Features of the Cytoskeleton of the Red Cell Membrane

D. Speicher, V. Marchesi, J. Morrow, 1982 .

Spectrin Domains: Proteolytic Susceptibility as a Probe of Protein Structure

D. Speicher, V. Marchesi, 1982, Journal of cellular biochemistry.

Repurposing Pan-HDAC Inhibitors for ARID1A-Mutated Ovarian Cancer

Benjamin G. Bitler, Lin Zhang, I. Shih, 2018, Cell reports.

Gamma-interferon-induced nerve growth factor receptors in colorectal carcinoma cell lines.

D. Speicher, W. Kaczmarski, E. Rakowicz-Szulczyńska, 1992, Growth factors.

Mitochondrial Akt Regulation of Hypoxic Tumor Reprogramming.

G. Lucignani, D. Speicher, J. C. Ghosh, 2016, Cancer cell.

Tropomodulin 3 Binds to Actin Monomers*

D. Speicher, V. Fowler, E. Yarmola, 2006, Journal of Biological Chemistry.

In-Depth, Label-Free Analysis of the Erythrocyte Cytoplasmic Proteome in Diamond Blackfan Anemia Identifies a Unique Inflammatory Signature

Esther N. Pesciotta, D. Speicher, A. Kossenkov, 2015, PloS one.

The host targeting motif in exported Plasmodium proteins is cleaved in the parasite endoplasmic reticulum.

K. Haldar, D. Speicher, S. Bhattacharjee, 2010, Molecular and biochemical parasitology.

Exosomal αvβ6 integrin is required for monocyte M2 polarization in prostate cancer.

D. Speicher, P. Weinreb, P. Fortina, 2018, Matrix biology : journal of the International Society for Matrix Biology.

Effect of glutathione on the phospholipase A2 activity of peroxiredoxin 6

D. Speicher, C. Dodia, A. Fisher, 2013 .

Structural and functional effects of hereditary hemolytic anemia-associated point mutations in the alpha spectrin tetramer site.

D. Speicher, P. Gallagher, S. Harper, 2008, Blood.

Pathogenic proline mutation in the linker between spectrin repeats: disease caused by spectrin unfolding.

D. Speicher, V. Ortiz, D. Discher, 2007, Blood.

LANDSCAPE OF THE MITOCHONDRIAL Hsp90 METABOLOME IN TUMORS

A. Tischler, D. Speicher, D. Wallace, 2013, Nature Communications.

F-actin affinity chromatography of detergent-solubilized plasma membranes: purification and initial characterization of ponticulin from Dictyostelium discoideum.

D. Speicher, A. Shariff, L. Wuestehube, 1991, Methods in enzymology.

Isolation of the Melanoma-Associated Antigen p23 Using Antibody Phage Display1

D. Elder, D. Speicher, P. V. Van Belle, 2001, The Journal of Immunology.

Biochemical Analysis of the Kruppel-associated Box (KRAB) Transcriptional Repression Domain

Sandra L. Harper, David W. Speicher, D. Speicher, 2000, The Journal of Biological Chemistry.

Antigen p 23 Using Antibody Phage Display Isolation of the Melanoma-Associated Herlyn

D. Elder, D. Speicher, P. V. Belle, 2000 .

BET Inhibitors Suppress ALDH Activity by Targeting ALDH1A1 Super-Enhancer in Ovarian Cancer.

Benjamin G. Bitler, Zhiguo Zhang, J. Bradner, 2016, Cancer research.

AKT PHOSPHORYLATION OF MITOCHONDRIAL LonP1 PROTEASE ENABLES OXIDATIVE METABOLISM AND ADVANCED TUMOR TRAITS

D. Speicher, J. C. Ghosh, D. Altieri, 2019, Oncogene.

Experimental Assignment of Disulfide‐Bonds in Purified Proteins

D. Speicher, Hsin-Yao Tang, 2019, Current protocols in protein science.

Remodeling of the malaria parasite and host human red cell by vesicle amplification that induces artemisinin resistance.

I. Coppens, K. Haldar, D. Speicher, 2018, Blood.

A multi-omics approach to Epstein-Barr virus immortalization of B-cells reveals EBNA1 chromatin pioneering activities targeting nucleotide metabolism.

Kyoung-Jae Won, D. Speicher, P. Lieberman, 2021, PLoS pathogens.

A Comprehensive Model of the Spectrin Divalent Tetramer Binding Region Deduced Using Homology Modeling and Chemical Cross-linking of a Mini-spectrin*

David W Speicher, D. Speicher, Hsin-Yao Tang, 2010, Journal of Biological Chemistry.

Immunogenicity of recombinant GA733‐2E antigen (CO17‐1A, EGP, KS1‐4, KSA, Ep‐CAM) in gastro‐intestinal carcinoma patients

D. Speicher, H. Braumüller, H. Beger, 2001, International journal of cancer.

Oligomeric State of the Colon Carcinoma-associated Glycoprotein GA733-2 (Ep-CAM/EGP40) and Its Role in GA733-mediated Homotypic Cell-Cell Adhesion*

J. M. Chong, D. Speicher, M. Trebak, 2001, The Journal of Biological Chemistry.

S100A14 Is Increased in Activated NK Cells and Plasma of HIV-Exposed Seronegative People Who Inject Drugs and Promotes Monocyte–NK Crosstalk

L. Montaner, D. Speicher, D. Metzger, 2019, Journal of acquired immune deficiency syndromes.

SWI/SNF catalytic subunits’ switch drives resistance to EZH2 inhibitors in ARID1A-mutated cells

Benjamin G. Bitler, Lin Zhang, T. Fukumoto, 2018, Nature Communications.

Three-dimensional culture sensitizes epithelial ovarian cancer cells to EZH2 methyltransferase inhibition

D. Speicher, Hua Li, Rugang Zhang, 2013, Cell cycle.

CLIC1 and CLIC4 complement CA125 as a diagnostic biomarker panel for all subtypes of epithelial ovarian cancer

D. Speicher, R. Drapkin, Rugang Zhang, 2018, Scientific Reports.

Cdc13 N-Terminal Dimerization, DNA Binding, and Telomere Length Regulation

E. Skordalakes, F. Johnson, D. Speicher, 2010, Molecular and Cellular Biology.

A systems biology analysis of metastatic melanoma using in‐depth three‐dimensional protein profiling

M. Herlyn, D. Speicher, Hsin-Yao Tang, 2010, Proteomics.

BAP1 mutant uveal melanoma is stratified by metabolic phenotypes with distinct vulnerability to metabolic inhibitors

D. Speicher, A. Aplin, J. Harbour, 2020, Oncogene.

LIMD2 is a small LIM-only protein overexpressed in metastatic lesions that regulates cell motility and tumor progression by directly binding to and activating the integrin-linked kinase.

Qihong Huang, M. Herlyn, E. Skordalakes, 2014, Cancer research.

Identification of a Survival-Promoting Peptide in Medium Conditioned by Oxidatively Stressed Cell Lines of Nervous System Origin

P. Levitt, D. Speicher, T. J. Cunningham, 1998, The Journal of Neuroscience.

Spectrin Motif: Conformation of a mammoth protein

D. Speicher, J. Ursitti, 1994, Current Biology.

The present status of erythrocyte spectrin structure: The 106‐residue repetitive structure is a basic feature of an entire class of proteins

D. Speicher, 1986, Journal of cellular biochemistry.

Microscale solution IEF combined with 2‐D DIGE substantially enhances analysis depth of complex proteomes such as mammalian cell and tissue extracts

M. Herlyn, D. Speicher, Mee‐Jung Han, 2008, Electrophoresis.

Concurrent MEK2 mutation and BRAF amplification confer resistance to BRAF and MEK inhibitors in melanoma.

Wei Xu, M. Herlyn, K. Nathanson, 2013, Cell reports.

KAP-1 Corepressor Interaction Is Essential − Silencing : Direct Chromoshadow Domain Heterochromatin Protein 1-Mediated Gene Molecular Determinants for Targeting

D. Speicher, M. Lechner, G. Begg, 2000 .

Strategies for Structural Analysis of Large Proteins: The Utility of Domains, Peptide Sequences, and Peptide Mapping

D. Speicher, 1987 .

The Mitochondrial Unfoldase-Peptidase Complex ClpXP Controls Bioenergetics Stress and Metastasis

D. Speicher, V. Vaira, S. Bosari, 2016, PLoS biology.

Introduction: CHEMICAL MICROSEQUENCING TECHNIQUES

D. Speicher, J. Shively, 1989 .

Label-free mass spectrometry exploits dozens of detected peptides to quantify lamins in wildtype and knockdown cells

D. Speicher, D. Discher, Jae-Won Shin, 2013, Nucleus.

Swift Matrix-Directed Differentiation Nuclear LaminA Scales with Tissue Stiffness and Enhances

D. Speicher, D. Discher, F. Rehfeldt, 2022 .

Initiation of Spectrin Dimerization Involves Complementary Electrostatic Interactions between Paired Triple-helical Bundles*

D. Speicher, M. Morris, G. Begg, 2000, The Journal of Biological Chemistry.

The complete cDNA and polypeptide sequences of human erythroid alpha-spectrin.

D. Speicher, V. Marchesi, T. Leto, 1990, The Journal of biological chemistry.

Proteome analysis of the triton-insoluble erythrocyte membrane skeleton.

Esther N. Pesciotta, D. Speicher, A. Chakrabarti, 2015, Journal of proteomics.

The exon 46-encoded sequence is essential for stability of human erythroid alpha-spectrin and heterodimer formation.

D. Speicher, J. Delaunay, S. Harper, 1997, Blood.

Properties of human red cell spectrin heterodimer (side-to-side) assembly and identification of an essential nucleation site.

D. Speicher, T. DeSilva, L. Wȩglarz, 1992, The Journal of biological chemistry.

Pathway shifts and thermal softening in temperature-coupled forced unfolding of spectrin domains.

D. Speicher, D. Discher, R. Law, 2003, Biophysical journal.

Poikilocytic hereditary elliptocytosis associated with spectrin Alexandria: an alpha I/50b Kd variant that is caused by a single amino acid deletion

D. Speicher, B. Forget, P. Gallagher, 1993 .

Influence of Lateral Association on Forced Unfolding of Antiparallel Spectrin Heterodimers*

Dennis E Discher, David W Speicher, D. Speicher, 2004, Journal of Biological Chemistry.

Role of terminal nonhomologous domains in initiation of human red cell spectrin dimerization.

D. Speicher, G. Begg, S. Harper, 2001, Biochemistry.

The common hereditary elliptocytosis-associated α-spectrin L260P mutation perturbs erythrocyte membranes by stabilizing spectrin in the closed dimer conformation.

D. Speicher, S. Sriswasdi, Hsin-Yao Tang, 2013, Blood.

RED CELLS , IRON , AND ERYTHROPOIESIS The common hereditary elliptocytosis-associated a-spectrin L 260 P mutation perturbs erythrocyte membranes by stabilizing spectrin in the closed dimer conformation

D. Speicher, S. Sriswasdi, Hsin-Yao Tang, 2013 .

Functional characterization of recombinant human red cell alpha-spectrin polypeptides containing the tetramer binding site.

D. Speicher, T. DeSilva, P. Curtis, 1993, The Journal of biological chemistry.

of CSF-1 R induced tumor infiltration by PMN-MDSC d Combination of CSF-1 R with CXCR 2 inhibitors demonstrated strong anti-tumor effect

Chunsheng Zhang, N. Hockstein, A. Weeraratna, 2017 .

Leronlimab, a humanized monoclonal antibody to CCR5, blocks breast cancer cellular metastasis and enhances cell death induced by DNA damaging chemotherapy

D. Ni, D. Speicher, R. Pestell, 2021, Breast cancer research : BCR.

Spectrin Tetramer Formation Is Not Required for Viable Development in Drosophila*

D. Speicher, Floyd J Mattie, G. Thomas, 2014, The Journal of Biological Chemistry.

INTERNAL PROTEIN SEQUENCING OF SDS PAGE-SEPARATED PROTEINS: A COLLABORATIVE ABRF STUDY

K. Williams, William Arbuthnot Sir Lane, U. Hellman, 1997 .

Amino acid sequence of the plasma membrane ATPase of Neurospora crassa: deduction from genomic and cDNA sequences.

D. Speicher, C. Slayman, J. Davenport, 1986, Proceedings of the National Academy of Sciences of the United States of America.

Protein isoform-specific validation defines multiple chloride intracellular channel and tropomyosin isoforms as serological biomarkers of ovarian cancer.

D. Speicher, Rugang Zhang, J. Tanyi, 2013, Journal of proteomics.

Chapter 1 – Overview of Proteome Analysis

D. Speicher, 2004 .

Two‐Dimensional Gel Electrophoresis

D. Speicher, S. Harper, S Harper, 1999, Current protocols in cell biology.

Two‐Dimensional Gel Electrophoresis

D. Speicher, S. Harper, J. Mozdzanowski, 1998, Current protocols in protein science.

A method for global analysis of complex proteomes using sample prefractionation by solution isoelectrofocusing prior to two-dimensional electrophoresis.

D. Speicher, X. Zuo, Xun Zuo, 2000, Analytical biochemistry.

Rapid verification of candidate serological biomarkers using gel-based, label-free multiple reaction monitoring.

K. Barnhart, D. Speicher, Hsin-Yao Tang, 2011, Journal of proteome research.

Complete amino acid sequence and homologies of human erythrocyte membrane protein band 4.2.

D. Speicher, J. Lawler, C. M. Cohen, 1990, Proceedings of the National Academy of Sciences of the United States of America.

Identification of Multiple Novel Protein Biomarkers Shed by Human Serous Ovarian Tumors into the Blood of Immunocompromised Mice and Verified in Patient Sera

D. Speicher, Rugang Zhang, Huan Wang, 2013, PloS one.

Peroxiredoxin 6 Phosphorylation and Subsequent Phospholipase A2 Activity Are Required for Agonist-mediated Activation of NADPH Oxidase in Mouse Pulmonary Microvascular Endothelium and Alveolar Macrophages*

D. Speicher, C. Dodia, A. Fisher, 2011, The Journal of Biological Chemistry.

Higher dimensional (Hi-D) separation strategies dramatically improve the potential for cancer biomarker detection in serum and plasma.

D. Speicher, L. Echan, Won-A. Joo, 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

In-Depth, Reproducible Analysis of Human Plasma Using IgY 14 and SuperMix Immunodepletion.

Bonnie Ky, David W Speicher, K. Barnhart, 2017, Methods in molecular biology.

Plasma biomarker discovery using 3D protein profiling coupled with label-free quantitation.

K. Barnhart, D. Speicher, Hsin-Yao Tang, 2011, Methods in molecular biology.

Comprehensive analysis of complex proteomes using microscale solution isoelectrofocusing prior to narrow pH range two‐dimensional electrophoresis

D. Speicher, X. Zuo, Xun Zuo, 2002, Proteomics.

Supervised Regularized Canonical Correlation Analysis: integrating histologic and proteomic measurements for predicting biochemical recurrence following prostate surgery

George Lee, Anant Madabhushi, Michael D. Feldman, 2011, BMC Bioinformatics.

Increased phospholipase A2 activity with phosphorylation of peroxiredoxin 6 requires a conformational change in the protein.

D. Speicher, C. Dodia, A. Fisher, 2012, Biochemistry.

Structure of the spectrin-actin binding site of erythrocyte protein 4.1.

D. Speicher, V. Marchesi, I. Correas, 1986, The Journal of biological chemistry.

The catalytic mechanism of the ESA1 histone acetyltransferase involves a self-acetylated intermediate

D. Speicher, R. Marmorstein, S. Harper, 2002, Nature Structural Biology.

Systematic discovery of ectopic pregnancy serum biomarkers using 3-D protein profiling coupled with label-free quantitation.

Sira Sriswasdi, David W Speicher, K. Barnhart, 2011, Journal of proteome research.

Comparison of Extensive Protein Fractionation and Repetitive LC-MS/MS Analyses on Depth of Analysis for Complex Proteomes

David W. Speicher, D. Speicher, Huan Wang, 2009, Journal of proteome research.

The HUPO Plasma Proteome Project: A report from the Munich congress

Gilbert S Omenn, Young-Ki Paik, David Speicher, 2006, Proteomics.

Human Serum and Plasma Proteomics

D. Speicher, Ian Musselman, 2005, Current protocols in protein science.

Protein Profiling by Microscale Solution Isoelectrofocusing (MicroSol‐IEF)

D. Speicher, X. Zuo, N. Ali-Khan, 2005, Current protocols in protein science.

Correction: Corrigendum: Landscape of the mitochondrial Hsp90 metabolome in tumours

A. Tischler, D. Speicher, D. Wallace, 2015, Nature Communications.

Amino Acid Analysis and Sequencing — What is State-of-the-Art?

K. Williams, D. Speicher, J. Crabb, 1991 .

Proteome Analysis Using Gel‐LC‐MS/MS

D. Speicher, Hsin-Yao Tang, P. Hembach, 2019, Current protocols in protein science.

Regulation of EBNA1 Protein Stability by PLOD1 Lysine Hydroxylase

D. Speicher, P. Lieberman, Andreas Wiedmer, 2022, bioRxiv.

Dematin and Adducin Provide a Novel Link between the Spectrin Cytoskeleton and Human Erythrocyte Membrane by Directly Interacting with Glucose Transporter-1*

D. Speicher, A. Chishti, Anwara . Khan, 2008, Journal of Biological Chemistry.

Cooperativity in forced unfolding of tandem spectrin repeats.

Dennis E Discher, David W Speicher, D. Speicher, 2003, Biophysical journal.

Baseline Immunoglobulin E Levels as a Marker of Doxorubicin- and Trastuzumab-Associated Cardiac Dysfunction.

D. Speicher, E. L. Luning Prak, S. Domchek, 2016, Circulation research.

The structurally disordered KRAB repression domain is incorporated into a protease resistant core upon binding to KAP-1-RBCC domain.

D. Speicher, K. Borden, R. Marmorstein, 2007, Journal of molecular biology.

Biosynthesis of the plasma membrane H+-ATPase of Neurospora crassa.

D. Speicher, C. Slayman, A. Chang, 1988, The Journal of biological chemistry.

Protein Detection in Gels Without Fixation

D. Speicher, Won-A. Joo, 1995, Current protocols in protein science.

Parkin ubiquitination of Kindlin-2 enables mitochondria-associated metastasis suppression

E. Skordalakes, D. Speicher, J. C. Ghosh, 2023, The Journal of biological chemistry.

A cancer ubiquitome landscape identifies metabolic reprogramming as target of Parkin tumor suppression

D. Speicher, J. C. Ghosh, V. Vaira, 2021, Science advances.

The mitophagy effector FUNDC1 controls mitochondrial reprogramming and cellular plasticity in cancer cells

D. Speicher, J. C. Ghosh, L. Languino, 2020, Science Signaling.

Syntaphilin Ubiquitination Regulates Mitochondrial Dynamics and Tumor Cell Movements.

Andrew R. Cohen, Eui Tae Kim, A. Cohen, 2018, Cancer research.

Erythrocyte detergent-resistant membrane proteins: their characterization and selective uptake during malarial infection.

P. Low, K. Haldar, D. Speicher, 2004, Blood.

Cloning of human erythroid dematin reveals another member of the villin family.

D. Speicher, A. Chishti, P. Ruff, 1993, Proceedings of the National Academy of Sciences of the United States of America.

SWI/SNF catalytic subunits’ switch drives resistance to EZH2 inhibitors in ARID1A-mutated cells

Lin Zhang, T. Fukumoto, D. Speicher, 2018, Nature Communications.

Molecular and Cellular Pathobiology LIMD 2 Is a Small LIM-Only Protein Overexpressed in Metastatic Lesions That Regulates Cell Motility and Tumor Progression by Directly Binding to and Activating the Integrin-Linked Kinase

Qihong Huang, M. Herlyn, E. Skordalakes, 2014 .

ARID1A spatially partitions interphase chromosomes

D. Speicher, Rugang Zhang, Hideki Tanizawa, 2019, Science Advances.

A. Weeraratna, D. Speicher, D. Wirtz, 2023, bioRxiv.

CARM1-expressing ovarian cancer depends on the histone methyltransferase EZH2 activity

D. Speicher, R. Drapkin, Rugang Zhang, 2018, Nature Communications.

Forced unfolding modulated by disulfide bonds in the Ig domains of a cell adhesion molecule.

D. Speicher, D. Discher, D E Discher, 2001, Proceedings of the National Academy of Sciences of the United States of America.

Determination of Disulfide‐Bond Linkages in Proteins

D. Speicher, Hsin-Yao Tang, 2004, Current protocols in protein science.

Towards global analysis of mammalian proteomes using sample prefractionation prior to narrow pH range two‐dimensional gels and using one‐dimensional gels for insoluble and large proteins

D. Speicher, X. Zuo, D. Santoli, 2001, Electrophoresis.

Nonenzymatic Protein Acetylation Detected by NAPPA Protein Arrays.

Joshua LaBaer, Ronen Marmorstein, David W Speicher, 2015, ACS chemical biology.

Probing large conformational rearrangements in wild-type and mutant spectrin using structural mass spectrometry

D. Speicher, S. Sriswasdi, Hsin-Yao Tang, 2014, Proceedings of the National Academy of Sciences.

Quantitative evaluation of protein recoveries in two‐dimensional electrophoresis with immobilized pH gradients

D. Speicher, X. Zuo, 2000, Electrophoresis.

Chemistry on a Single Protein, Vascular Cell Adhesion Molecule-1, during Forced Unfolding*

Hui Lu, Dennis E Discher, David W Speicher, 2004, Journal of Biological Chemistry.

Proteomics: an infinite problem with infinite potential. (Opinion)

David W. Speicher, D. Speicher, 2002 .

Forced Unfolding of Proteins Within Cells

D. Speicher, D. Discher, Hsin-Yao Tang, 2007, Science.

Cysteine shotgun–mass spectrometry (CS-MS) reveals dynamic sequence of protein structure changes within mutant and stressed cells

D. Speicher, D. Discher, N. Mohandas, 2011, Proceedings of the National Academy of Sciences.

A label-free proteome analysis strategy for identifying quantitative changes in erythrocyte membranes induced by red cell disorders.

Esther N. Pesciotta, D. Speicher, S. Sriswasdi, 2012, Journal of proteomics.

Molecular basis and haplotyping of the alphaII domain polymorphisms of spectrin: application to the study of hereditary elliptocytosis and pyropoikilocytosis.

D. Speicher, B. Forget, P. Gallagher, 1996, American journal of human genetics.

A Structural Model of the Erythrocyte Spectrin Heterodimer Initiation Site Determined Using Homology Modeling and Chemical Cross-linking*

David W Speicher, D. Speicher, Hsin-Yao Tang, 2008, Journal of Biological Chemistry.

disease caused by spectrin unfolding Pathogenic proline mutation in the linker between spectrin repeats

D. Speicher, V. Ortiz, D. Discher, 2009 .

A Fused α-β “Mini-spectrin” Mimics the Intact Erythrocyte Spectrin Head-to-head Tetramer*

D. Speicher, P. Gallagher, S. Harper, 2010, The Journal of Biological Chemistry.

Fractionation of Complex Proteomes by Microscale Solution Isoelectrofocusing Using ZOOM− IEF Fractionators to Improve Protein Profiling

D. Speicher, X. Zuo, Ki-Boom Lee, 2005 .

Full-Length Anion Exchanger 1 Structure and Interactions with Ankyrin-1 Determined by Zero Length Crosslinking of Erythrocyte Membranes.

D. Speicher, S. Sriswasdi, S. Harper, 2017, Structure.

Quantitative Comparisons of Large Numbers of Human Plasma Samples Using TMT10plex Labeling.

K. Barnhart, D. Speicher, B. Ky, 2017, Methods in molecular biology.

Cloning of a portion of the chromosomal gene for human erythrocyte alpha-spectrin by using a synthetic gene fragment.

D. Speicher, V. Marchesi, B. Forget, 1986, Proceedings of the National Academy of Sciences of the United States of America.

Structure of human erythrocyte spectrin. I. Isolation of the alpha-I domain and its cyanogen bromide peptides.

D. Speicher, V. Marchesi, P. Yurchenco, 1983, The Journal of biological chemistry.

Poikilocytic hereditary elliptocytosis associated with spectrin Alexandria: an alpha I/50b Kd variant that is caused by a single amino acid deletion.

D. Speicher, B. Forget, P. Gallagher, 1993, Blood.

Peroxiredoxin 6 homodimerization and heterodimerization with glutathione S-transferase pi are required for its peroxidase but not phospholipase A2 activity.

D. Speicher, C. Dodia, A. Fisher, 2016, Free radical biology & medicine.

Electroblotting from Polyacrylamide Gels

D. Speicher, J. Ursitti, J. Mozdzanowski, 1995, Current protocols in protein science.

Depletion of multiple high‐abundance proteins improves protein profiling capacities of human serum and plasma

D. Speicher, Hsin-Yao Tang, N. Ali-Khan, 2005, Proteomics.

Using Sample Prefractionation by Solution Isoelectrofocusing Prior to Two-Dimensional Electrophoresis

D. Speicher, X. Zuo, Xun Zuo, 2000 .

Stromal changes in the aged lung induce an emergence from melanoma dormancy

Stephen M. Douglass, A. Weeraratna, E. Jaffee, 2022, Nature.

Physical properties of a single-motif erythrocyte spectrin peptide: a highly stable independently folding unit.

D. Speicher, L. Otvos, T. DeSilva, 1997, Biochemistry.

Forced Unfolding Modulated by Disulfide Bonds in the Immunoglobulin Domains of the Cell Adhesion Molecule Mel-CAM.

Dennis E. Discher, David W. Speicher, D. Speicher, 2001 .

Regulation of EBNA1 protein stability and DNA replication activity by PLOD1 lysine hydroxylase

D. Speicher, P. Lieberman, Andreas Wiedmer, 2023, PLoS pathogens.

Characterization of tenascin secreted by human melanoma cells.

D. Guerry, M. Herlyn, D. Speicher, 1991, Cancer research.

Data analysis strategy for maximizing high-confidence protein identifications in complex proteomes such as human tumor secretomes and human serum.

D. Speicher, Huan Wang, Hsin-Yao Tang, 2011, Journal of proteome research.

Electrophoretic Prefractionation for Comprehensive Analysis of Proteomes

D. Speicher, KiBeom Lee, Xun Zuo, 2004 .

MYC regulates fatty acid metabolism through a multigenic program in claudin-low triple negative breast cancer

D. Speicher, E. Gottlieb, P. Adams, 2020, British Journal of Cancer.

Colorectal Cancer Vaccines: Antiidiotypic Antibody, Recombinant Protein, and Viral Vector

D. Speicher, R. Gutzmer, E. Mitchell, 2000, Annals of the New York Academy of Sciences.

Initiation and propagation of spectrin heterodimer assembly involves distinct energetic processes.

D. Speicher, S. Harper, Donghai Li, 2007, Biochemistry.

Identification of functional domains of human erythrocyte spectrin.

D. Speicher, V. Marchesi, J. Morrow, 1980, Proceedings of the National Academy of Sciences of the United States of America.

PI(3)P-independent and -dependent pathways function together in a vacuolar translocation sequence to target malarial proteins to the host erythrocyte.

K. Haldar, D. Speicher, R. Stahelin, 2012, Molecular and biochemical parasitology.

Lysosomal lipid peroxidation regulates tumor immunity.

D. Speicher, A. Aplin, J. Winkler, 2023, Journal of Clinical Investigation.

Colorectal carcinoma invasion inhibition by CO17-1A/GA733 antigen and its murine homologue.

D. Speicher, G. Maul, G. Maul, 1998, Journal of the National Cancer Institute.

Secretagogue-induced proteolysis of lung spectrin in alveolar epithelial type II cells.

D. Speicher, A. Fisher, U. Zimmerman, 1992, Biochimica et biophysica acta.

D. Speicher, C. Dodia, A. Fisher, 2011, The Journal of Biological Chemistry.

Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration.

John D Lambris, D. Speicher, J. Lambris, 2014, Human molecular genetics.

A tight junction-associated Merlin-angiomotin complex mediates Merlin's regulation of mitogenic signaling and tumor suppressive functions.

A. Stemmer-Rachamimov, D. Speicher, J. Kissil, 2011, Cancer cell.

Microscale solution isoelectrofocusing: a sample prefractionation method for comprehensive proteome analysis.

Xun Zuo, David W Speicher, D. Speicher, 2004, Methods in molecular biology.

Solution structure of the reduced form of human peroxiredoxin-6 elucidated using zero-length chemical cross-linking and homology modelling.

D. Speicher, A. Fisher, S. Sriswasdi, 2015, The Biochemical journal.

Probing large conformational rearrangements in wild-type and mutant spectrin using structural mass spectrometry

D. Speicher, S. Sriswasdi, Hsin-Yao Tang, 2014, Proceedings of the National Academy of Sciences.

The common hereditary elliptocytosis-associated α-spectrin L260P mutation perturbs erythrocyte membranes by stabilizing spectrin in the closed dimer conformation.

D. Speicher, S. Sriswasdi, Hsin-Yao Tang, 2013, Blood.

In vivo phosphorylation of human erythrocyte spectrin occurs in a sequential manner.

D. Speicher, Hsin-Yao Tang, 2004, Biochemistry.

Purification of human smooth muscle filamin and characterization of structural domains and functional sites.

D. Speicher, R. Hock, G. Davis, 1990, Biochemistry.

Peroxiredoxin 6 homodimerization and heterodimerization with glutathione S-transferase pi are required for its peroxidase but not phospholipase A2 activity.

D. Speicher, C. Dodia, A. Fisher, 2016, Free radical biology & medicine.

Mitogen-activated protein kinase-mediated phosphorylation of peroxiredoxin 6 regulates its phospholipase A(2) activity.

D. Speicher, C. Dodia, A. Fisher, 2009, The Biochemical journal.

Three-dimensional culture sensitizes epithelial ovarian cancer cells to EZH2 methyltransferase inhibition

D. Speicher, Hua Li, Rugang Zhang, 2013, Cell cycle.

A Comprehensive Model of the Spectrin Divalent Tetramer Binding Region Deduced Using Homology Modeling and Chemical Cross-linking of a Mini-spectrin*

D. Speicher, Hsin-Yao Tang, P. Gallagher, 2010, The Journal of Biological Chemistry.

A method for high-performance sequence analysis using polyvinylidene difluoride membranes with a biphasic reaction column sequencer.

D. Speicher, D. Reim, 1994, Analytical biochemistry.

Targeting UGCG overcomes resistance to lysosomal autophagy inhibition.

P. Gimotty, M. Herlyn, H. Stark, 2022, Cancer discovery.