Huimin Zhao

发表

A highly sensitive selection method for directed evolution of homing endonucleases

Zhilei Chen, Huimin Zhao, 2005, Nucleic acids research.

Customized optimization of metabolic pathways by combinatorial transcriptional engineering

Huimin Zhao, Jing Du, Yongbo Yuan, 2012, Nucleic acids research.

Divalent cations promote TALE DNA-binding specificity

Huimin Zhao, C. Schroeder, D. Shukla, 2019, Nucleic acids research.

Directed evolution of phloroglucinol synthase PhlD with increased stability for phloroglucinol production

Huimin Zhao, Jung-Kul Lee, G. Rao, 2013, Applied Microbiology and Biotechnology.

Recent advances in metabolic engineering of Saccharomyces cerevisiae: New tools and their applications.

Huimin Zhao, J. Lian, Shekhar Mishra, 2018, Metabolic engineering.

A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data

Hoang V. Dinh, C. V. Rao, C. Maranas, 2019, bioRxiv.

A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data

Hoang V. Dinh, C. V. Rao, C. Maranas, 2019, bioRxiv.

Computational Tools for Discovering and Engineering Natural Product Biosynthetic Pathways

Huimin Zhao, Hengqian Ren, Chengyou Shi, 2019, iScience.

Direct cloning of large genomic sequences

Huimin Zhao, R. Cobb, 2012, Nature Biotechnology.

Biocatalysis for the synthesis of pharmaceuticals and pharmaceutical intermediates.

Huimin Zhao, Huihua Sun, Hongfang Zhang, 2017, Bioorganic & medicinal chemistry.

Expanding the boundary of biocatalysis: design and optimization of in vitro tandem catalytic reactions for biochemical production

Huimin Zhao, Hengqian Ren, Yajie Wang, 2018, Critical reviews in biochemistry and molecular biology.

Cloning, characterization, and engineering of fungal L-arabinitol dehydrogenases

Huimin Zhao, Byoungjin Kim, R. Sullivan, 2010, Applied Microbiology and Biotechnology.

High Throughput Screening and Selection Methods for Directed Enzyme Evolution

Huimin Zhao, Han Xiao, Zehua Bao, 2014, Industrial & engineering chemistry research.

Metabolic engineering of a Saccharomyces cerevisiae strain capable of simultaneously utilizing glucose and galactose to produce enantiopure (2R,3R)-butanediol.

Huimin Zhao, J. Lian, Ran Chao, 2014, Metabolic engineering.

Protein design for pathway engineering.

Huimin Zhao, J. Lian, Dawn T. Eriksen, 2014, Journal of structural biology.

Regeneration of cofactors for use in biocatalysis.

Huimin Zhao, W. A. van der Donk, 2003, Current opinion in biotechnology.

Directed evolution of a cellobiose utilization pathway in Saccharomyces cerevisiae by simultaneously engineering multiple proteins

Huimin Zhao, Dawn T. Eriksen, Pei Chiun Helen Hsieh, 2013, Microbial Cell Factories.

Emerging molecular biology tools and strategies for engineering natural product biosynthesis

Huimin Zhao, W. Xu, E. Ang, 2019, Metabolic engineering communications.

Unlocking nature's biosynthetic potential by directed genome evolution.

Huimin Zhao, Mingfeng Cao, V. G. Tran, 2020, Current opinion in biotechnology.

A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae.

Mingzi M. Zhang, Huimin Zhao, E. Ang, 2016, Metabolic engineering.

Engineering microbial hosts for production of bacterial natural products.

Mingzi M. Zhang, Huimin Zhao, E. Ang, 2016, Natural product reports.

A New Era of Genome Integration-Simply Cut and Paste!

Huimin Zhao, E. Ang, Zihe Liu, 2017, ACS synthetic biology.

Utilizing an endogenous pathway for 1-butanol production in Saccharomyces cerevisiae.

Huimin Zhao, Tong Si, Yunzi Luo, 2014, Metabolic engineering.

Genome-wide RNAi screen reveals the E3 SUMO-protein ligase gene SIZ1 as a novel determinant of furfural tolerance in Saccharomyces cerevisiae

Huimin Zhao, Han Xiao, 2014, Biotechnology for Biofuels.

Transcription activator‐like effector nucleases (TALENs): A highly efficient and versatile tool for genome editing

Huimin Zhao, N. Sun, 2013, Biotechnology and bioengineering.

Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides.

Huimin Zhao, Mingfeng Cao, J. Schultz, 2019, Biotechnology and bioengineering.

Genome-Scale Consequences of Cofactor Balancing in Engineered Pentose Utilization Pathways in Saccharomyces cerevisiae

N. Price, Amit Ghosh, Huimin Zhao, 2011, PloS one.

Rapid prototyping of microbial cell factories via genome-scale engineering.

Huimin Zhao, Tong Si, Huimin Zhao, 2015, Biotechnology advances.

Relaxing the nicotinamide cofactor specificity of phosphite dehydrogenase by rational design.

Huimin Zhao, Wilfred A van der Donk, Ryan Woodyer, 2003, Biochemistry.

Molecular evolution by staggered extension process (StEP) in vitro recombination

Frances H. Arnold, Huimin Zhao, F. Arnold, 1998, Nature Biotechnology.

Directed Enzyme Evolution and High-Throughput Screening

Huimin Zhao, Huimin Zhao, R. Sullivan, 2009 .

Directed evolution: an evolving and enabling synthetic biology tool.

Huimin Zhao, Tong Si, Ryan E Cobb, 2012, Current opinion in chemical biology.

Biocatalyst development by directed evolution.

Meng Wang, Huimin Zhao, Tong Si, 2012, Bioresource technology.

Directed evolution as a powerful synthetic biology tool.

Huimin Zhao, Ryan E Cobb, Huimin Zhao, 2013, Methods.

Directed evolution of orthogonal ligand specificity in a single scaffold.

Huimin Zhao, M. McLachlan, K. Chockalingam, 2009, Angewandte Chemie.

Activation and Characterization of a Cryptic Polycyclic Tetramate Macrolactam Biosynthetic Gene Cluster

Meng Wang, Huimin Zhao, Yunzi Luo, 2013, Nature Communications.

Systematic Identification of a Panel of Strong Constitutive Promoters from Streptomyces albus.

Huimin Zhao, Lu Zhang, Yunzi Luo, 2015, ACS synthetic biology.

New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters.

Huimin Zhao, Behnam Enghiad, Yunzi Luo, 2016, Natural product reports.

Standardization for natural product synthetic biology.

Huimin Zhao, M. Medema, 2016, Natural product reports.

DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

Zengyi Shao, Huimin Zhao, Hua Zhao, 2008, Nucleic acids research.

Iowa State University From the SelectedWorks of Zengyi Shao 2012 Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae

Huimin Zhao, Nikhil U. Nair, Hua Zhao, 2017 .

Direct Conversion of Xylan to Ethanol by Recombinant Saccharomyces cerevisiae Strains Displaying an Engineered Minihemicellulosome

Huimin Zhao, Tong Si, Fei Wen, 2012, Applied and Environmental Microbiology.

' s personal copy Protein design for pathway engineering

Huimin Zhao, J. Lian, Dawn T. Eriksen, 2014 .

Recent advances in DNA assembly technologies.

Huimin Zhao, Ran Chao, Huimin Zhao, 2014, FEMS yeast research.

Rapid and efficient galactose fermentation by engineered Saccharomyces cerevisiae.

Jeffrey M. Skerker, A. Arkin, Huimin Zhao, 2016, Journal of biotechnology.

Investigating glucose and xylose metabolism in Saccharomyces cerevisiae and Scheffersomyces stipitis via 13C metabolic flux analysis

Huimin Zhao, Xueyang Feng, 2013 .

Genome-scale metabolic reconstruction of the non-model yeast Issatchenkia orientalis SD108 and its application to organic acids production

Hoang V. Dinh, C. Maranas, J. Rabinowitz, 2020, Metabolic engineering communications.

RNAi-assisted genome evolution in Saccharomyces cerevisiae for complex phenotype engineering.

Huimin Zhao, Zehua Bao, Tong Si, 2015, ACS synthetic biology.

Construction of plasmids with tunable copy numbers in Saccharomyces cerevisiae and their applications in pathway optimization and multiplex genome integration

Huimin Zhao, J. Lian, Run Jin, 2016, Biotechnology and bioengineering.

Engineered CRISPR/Cas9 system for multiplex genome engineering of polyploid industrial yeast strains.

Huimin Zhao, J. Lian, Zehua Bao, 2018, Biotechnology and bioengineering.

Directed evolution of a highly efficient cellobiose utilizing pathway in an industrial Saccharomyces cerevisiae strain

Huimin Zhao, Yongbo Yuan, 2013, Biotechnology and bioengineering.

Directed evolution of a cellodextrin transporter for improved biofuel production under anaerobic conditions in Saccharomyces cerevisiae

Huimin Zhao, Jiazhang Lian, Mohammad HamediRad, 2014, Biotechnology and bioengineering.

Reversal of the β-oxidation cycle in Saccharomyces cerevisiae for production of fuels and chemicals.

Huimin Zhao, Jiazhang Lian, Huimin Zhao, 2015, ACS synthetic biology.

Regulatory RNA-assisted genome engineering in microorganisms.

Huimin Zhao, Mohammad HamediRad, Tong Si, 2015, Current opinion in biotechnology.

Homology-integrated CRISPR-Cas (HI-CRISPR) system for one-step multigene disruption in Saccharomyces cerevisiae.

Huimin Zhao, Lu Zhang, Zehua Bao, 2015, ACS synthetic biology.

Applying Advanced DNA Assembly Methods to Generate Pathway Libraries

Huimin Zhao, Dawn T. Eriksen, Ran Chao, 2018 .

Combinatorial pathway engineering for optimized production of the anti‐malarial FR900098

Huimin Zhao, Todd Freestone, 2016, Biotechnology and bioengineering.

Automated multiplex genome-scale engineering in yeast

Huimin Zhao, Tong Si, Ran Chao, 2017, Nature Communications.

Engineering robust microorganisms for organic acid production

Huimin Zhao, V. G. Tran, V. Tran, 2021, Journal of industrial microbiology & biotechnology.

Recent advances in domesticating non‐model microorganisms

Huimin Zhao, Z. Fatma, Carl Schultz, 2020, Biotechnology progress.

Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping

Huimin Zhao, J. Lian, Mingfeng Cao, 2019, Nature Communications.

RNAi assisted genome evolution unveils yeast mutants with improved xylose utilization

Huimin Zhao, J. Lian, Hejun Li, 2018, Biotechnology and bioengineering.

Discovery and engineering of a 1-butanol biosensor in Saccharomyces cerevisiae.

Huimin Zhao, Ee Lui Ang, Huimin Zhao, 2017, Bioresource technology.

Yeast Surface Display of Trifunctional Minicellulosomes for Simultaneous Saccharification and Fermentation of Cellulose to Ethanol

Huimin Zhao, Fei Wen, Huimin Zhao, 2009, Applied and Environmental Microbiology.

Combinatorial Design of a Highly Efficient Xylose-Utilizing Pathway in Saccharomyces cerevisiae for the Production of Cellulosic Biofuels

Huimin Zhao, Jing Du, Byoungjin Kim, 2012, Applied and Environmental Microbiology.

FairyTALE: a high-throughput TAL effector synthesis platform.

Huimin Zhao, Zhanar Abil, Zehua Bao, 2014, ACS synthetic biology.

Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis

Huimin Zhao, Xiaofei Song, Z. Fatma, 2019, mSphere.

A plug‐and‐play pathway refactoring workflow for natural product research in Escherichia coli and Saccharomyces cerevisiae

Huimin Zhao, Hengqian Ren, Pingfan Hu, 2017, Biotechnology and bioengineering.

Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method

Huimin Zhao, Zihe Liu, Huimin Zhao, 2017, Nucleic acids research.

Accelerated genome engineering through multiplexing

Huimin Zhao, Zehua Bao, Ryan E Cobb, 2016, Wiley interdisciplinary reviews. Systems biology and medicine.

Optimized TAL effector nucleases (TALENs) for use in treatment of sickle cell disease.

Huimin Zhao, Zhanar Abil, Jing Liang, 2012, Molecular bioSystems.

Coordinated induction of multi-gene pathways in Saccharomyces cerevisiae

Huimin Zhao, J. C. Ning, Huimin Zhao, 2012, Nucleic acids research.

Creating new specific ligand-receptor pairs for transgene regulation.

Huimin Zhao, Karuppiah Chockalingam, Huimin Zhao, 2005, Trends in biotechnology.

Distinct structural elements dictate the specificity of the type III pentaketide synthase from Neurospora crassa.

Huimin Zhao, S. Nair, J. Brunzelle, 2008, Chemistry & biology.

Selective reduction of xylose to xylitol from a mixture of hemicellulosic sugars.

Huimin Zhao, Nikhil U. Nair, Nikhil U Nair, 2010, Metabolic engineering.

Directed evolution of enzymes and pathways for industrial biocatalysis.

Huimin Zhao, Zhilei Chen, Karuppiah Chockalingam, 2002, Current opinion in biotechnology.

Direct observation of TALE protein dynamics reveals a two-state search mechanism

Huimin Zhao, C. Schroeder, Luke Cuculis, 2015, Nature Communications.

CTCF Promotes Long-range Enhancer-promoter Interactions and Lineage-specific Gene Expression in Mammalian Cells

Jesse R. Dixon, B. Ren, D. Gorkin, 2020, bioRxiv.

Integrating biocatalysis with chemocatalysis for selective transformations.

Huimin Zhao, Mingfeng Cao, Xiaoqiang Huang, 2020, Current opinion in chemical biology.

Production of Adipic Acid from Sugar Beet Residue by Combined Biological and Chemical Catalysis

Huimin Zhao, Yugen Zhang, Hongfang Zhang, 2015 .

Efficient regeneration of NADPH using an engineered phosphite dehydrogenase

Huimin Zhao, Tyler W Johannes, Ryan D. Woodyer, 2007, Biotechnology and bioengineering.

Author ' s personal copy Biocatalyst development by directed evolution

M. Wang, Huimin Zhao, Tong Si, 2012 .

Cloning and characterization of a type III polyketide synthase from Aspergillus niger.

Huimin Zhao, Jung-Kul Lee, Yunzi Luo, 2011, Bioorganic & medicinal chemistry letters.

The Botrytis cinerea type III polyketide synthase shows unprecedented high catalytic efficiency toward long chain acyl-CoAs.

Huimin Zhao, M. Jeya, Jung-Kul Lee, 2012, Molecular bioSystems.

Drug Discovery and Development via Synthetic Biology

Huimin Zhao, R. Cobb, Yunzi Luo, 2013 .

An efficient gene knock-in strategy using 5’-modified dsDNA donors with short homology arms

Huimin Zhao, Yi-Zun Yu, Yijun Guo, 2019, Nature Chemical Biology.

Using natural products for drug discovery: the impact of the genomics era

Mingzi M. Zhang, Huimin Zhao, Yuan Qiao, 2017, Expert opinion on drug discovery.

Auroramycin: A Potent Antibiotic from Streptomyces roseosporus by CRISPR‐Cas9 Activation

Mingzi M. Zhang, Shawn Hoon, Huimin Zhao, 2018, Chembiochem : a European journal of chemical biology.

Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae.

Huimin Zhao, Jamie H. D. Cate, Jonathan M Galazka, 2010, Molecular bioSystems.

Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering.

Huimin Zhao, Zengyi Shao, Sheryl B. Rubin-pitel, 2009, Metabolic engineering.

A genetic toolbox for metabolic engineering of Issatchenkia orientalis.

Huimin Zhao, Y. Yoshikuni, Xiaofei Song, 2020, Metabolic engineering.

A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

Huimin Zhao, Guanhua Xun, S. Lane, 2021, Nature Communications.

Investigation of the Role of Arg301 Identified in the X-ray Structure of Phosphite Dehydrogenase

Huimin Zhao, W. Metcalf, W. A. van der Donk, 2012, Biochemistry.

Optimizing a biocatalyst for improved NAD(P)H regeneration: directed evolution of phosphite dehydrogenase.

Huimin Zhao, W. A. van der Donk, Ryan D. Woodyer, 2006, Combinatorial chemistry & high throughput screening.

Seamless correction of the sickle cell disease mutation of the HBB gene in human induced pluripotent stem cells using TALENs.

Huimin Zhao, N. Sun, 2014, Biotechnology and bioengineering.

Visualizing Spatiotemporal Dynamics of Intercellular Mechanotransmission upon Wounding.

Linda Z. Shi, Huimin Zhao, M. Berns, 2018, ACS photonics.

CrystalStructureandComputationalAnalysesProvideInsights intotheCatalyticMechanismof2,4-Diacetylphloroglucinol HydrolasePhlGfromPseudomonasfluorescens *

Huimin Zhao, Cong-Zhao Zhou, Y. Teng, 2010 .

In vitro reconstitution and crystal structure of p-aminobenzoate N-oxygenase (AurF) involved in aureothin biosynthesis

Huimin Zhao, S. Nair, Y. Choi, 2008, Proceedings of the National Academy of Sciences.

Strain Improvement via Evolutionary Engineering

Huimin Zhao, Jing Du, Byoungjin Kim, 2012 .

Characterization of Bacillus subtilis Colony Biofilms via Mass Spectrometry and Fluorescence Imaging

Huimin Zhao, Tong Si, J. Sweedler, 2016, Journal of proteome research.

Improving the thermostability of lipase Lip2 from Yarrowia lipolytica.

T. Tan, Huimin Zhao, Sai Wen, 2013, Journal of biotechnology.

Directed Evolution of a Fluorinase for Improved Fluorination Efficiency with a Non-native Substrate.

Huimin Zhao, R. Robinson, Huihua Sun, 2016, Angewandte Chemie.

A diverse family of Type III polyketide synthases in Eucalyptus species.

Huimin Zhao, Jung-Kul Lee, Yunzi Luo, 2010, Molecular bioSystems.

Development of a Synthetic Malonyl-CoA Sensor in Saccharomyces cerevisiae for Intracellular Metabolite Monitoring and Genetic Screening.

M. Wang, Huimin Zhao, Tong Si, 2015, ACS synthetic biology.

A new biosensor for stilbenes and a cannabinoid enabled by genome mining of a transcriptional regulator.

Huimin Zhao, Huihua Sun, E. Ang, 2020, ACS synthetic biology.

Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion.

Huimin Zhao, J. Lian, Carl Schultz, 2018, Methods in enzymology.

Metabolic engineering of Rhodotorula toruloides IFO0880 improves C16 and C18 fatty alcohol production from synthetic media

Hoang V. Dinh, C. Maranas, Huimin Zhao, 2022, Microbial Cell Factories.

Rapid Screening of Lanthipeptide Analogs via In-Colony Removal of Leader Peptides in Escherichia coli.

Huimin Zhao, Tong Si, J. Sweedler, 2018, Journal of the American Chemical Society.

Insights into Cell-Free Conversion of CO2 to Chemicals by a Multienzyme Cascade Reaction

Dong Rip Kim, S. Kondaveeti, Byung-Kwan Cho, 2018, ACS Catalysis.

Cloning, characterization, and mutational analysis of a highly active and stable l-arabinitol 4-dehydrogenase from Neurospora crassa

Huimin Zhao, R. Sullivan, 2007, Applied Microbiology and Biotechnology.

Biochemical Characterization of an l-Xylulose Reductase from Neurospora crassa

Nikhil U. Nair, Huimin Zhao, 2007, Applied and Environmental Microbiology.

para‐Aminobenzoate N‐Oxygenase

Huimin Zhao, S. Nair, 2009 .

Probing the Substrate Specificity of Aminopyrrolnitrin Oxygenase (PrnD) by Mutational Analysis

Huimin Zhao, Jung-Kul Lee, E. Ang, 2006, Journal of bacteriology.

Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria

Huimin Zhao, E. Ang, Z. Yuchi, 2019, Nature Communications.

A Pathway for Isethionate Dissimilation in Bacillus krulwichiae

Huimin Zhao, E. Ang, Yiling Hu, 2019, Applied and Environmental Microbiology.

Identiﬁcation and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Biﬁdobacterium kashiwanohense

Huimin Zhao, E. Ang, Z. Yuchi, 2022 .

Directed evolution of specific receptor-ligand pairs for use in the creation of gene switches.

Huimin Zhao, Zhilei Chen, Karuppiah Chockalingam, 2005, Proceedings of the National Academy of Sciences of the United States of America.

Directed evolution of homing endonuclease with novel DNA sequence specificity

Zhilei Chen, Huimin Zhao, 2005 .

Discovery and Characterization of a Class IV Lanthipeptide with a Non-overlapping Ring Pattern.

Huimin Zhao, Hengqian Ren, Chengyou Shi, 2020, ACS chemical biology.

A scalable platform to discover antimicrobials of ribosomal origin

Huimin Zhao, Hengqian Ren, Chengyou Shi, 2022, Nature Communications.

Directed Evolution to Engineer Monobody for FRET Biosensor Assembly and Imaging at Live-Cell Surface.

A. Strongin, Huimin Zhao, B. Kay, 2018, Cell chemical biology.

Structural and Biochemical Investigation of UTP Cyclohydrolase

Huimin Zhao, E. Ang, P. Cao, 2021, ACS Catalysis.

Proteome capacity constraints favor respiratory ATP generation

Hoang V. Dinh, C. Maranas, J. Rabinowitz, 2022, bioRxiv.

A New Class of Arylamine Oxygenases: Evidence that p‐Aminobenzoate N‐Oxygenase (AurF) is a Di‐iron Enzyme and Further Mechanistic Studies

Huimin Zhao, M. Simurdiak, Jungkul Lee, 2006, Chembiochem : a European journal of chemical biology.

Biosynthetic engineering of the antifungal, anti-MRSA auroramycin

Mingzi M. Zhang, Huimin Zhao, E. Ang, 2020, Microbial cell factories.

A step through the looking glass

Huimin Zhao, E. Ang, Youyun Liang, 2016, Science China Life Sciences.

Comparative study of two Saccharomyces cerevisiae strains with kinetic models at genome-scale.

Hoang V. Dinh, C. Maranas, J. Rabinowitz, 2023, Metabolic engineering.

Anaerobic Hydroxyproline Degradation Involving C-N Cleavage by a Glycyl Radical Enzyme.

Huimin Zhao, E. Ang, Z. Yuchi, 2022, Journal of the American Chemical Society.

Chromatin architecture reorganization during stem cell differentiation

Jing Liang, Zhen Ye, Huimin Zhao, 2015, Nature.

Unraveling the iterative type I polyketide synthases hidden in Streptomyces

Huimin Zhao, Chunshuai Huang, Fang Guo, 2020, Proceedings of the National Academy of Sciences.

Biocatalysis for Drug Discovery and Development

Mingzi M. Zhang, Huimin Zhao, E. Ang, 2016 .

Exploiting Issatchenkia orientalis SD108 for succinic acid production

Huimin Zhao, Zengyi Shao, Huimin Zhao, 2014, Microbial Cell Factories.

Directed evolution of enzymes and biosynthetic pathways.

Huimin Zhao, Huimin Zhao, Tyler W Johannes, 2006, Current opinion in microbiology.

Investigation of the functional role of aldose 1-epimerase in engineered cellobiose utilization.

Jamie H. D. Cate, Huimin Zhao, Heejin Kim, 2013, Journal of biotechnology.

Metabolic Engineering of Oleaginous Yeasts for Production of Fuels and Chemicals

Huimin Zhao, Huimin Zhao, S. Shi, 2017, Front. Microbiol..

Directed Evolution: Past, Present and Future.

Huimin Zhao, Ran Chao, Ryan E Cobb, 2013, AIChE journal. American Institute of Chemical Engineers.

In vitro 'sexual' evolution through the PCR-based staggered extension process (StEP)

Huimin Zhao, Wenjuan Zha, 2006, Nature Protocols.

Selective Elimination of Mitochondrial Mutations in the Germline by Genome Editing

J. C. Belmonte, I. Sancho-Martinez, Keiichiro Suzuki, 2015, Cell.

Metabolic engineering of threonine catabolism enables Saccharomyces cerevisiae to produce propionate under aerobic conditions

Huimin Zhao, S. Shi, Genlai Dong, 2022, Biotechnology journal.

Profiling of d‐alanine production by the microbial isolates of rat gut microbiota

Huimin Zhao, Tong Si, J. Sweedler, 2021, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

Heterologous production of fosfomycin and identification of the minimal biosynthetic gene cluster.

N. Kelleher, Huimin Zhao, P. Thomas, 2006, Chemistry & biology.

New insight into the mechanism of methyl transfer during the biosynthesis of fosfomycin.

Huimin Zhao, W. A. van der Donk, Ryan D. Woodyer, 2007, Chemical communications.

Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition

Huimin Zhao, D. Silverman, H. Nick, 2006, The FEBS journal.

Cloning, expression, and biochemical characterization of Streptomyces rubellomurinus genes required for biosynthesis of antimalarial compound FR900098.

N. Kelleher, B. Griffin, Huimin Zhao, 2008, Chemistry & biology.

High‐Throughput Mass Spectrometry Complements Protein Engineering

Huimin Zhao, Tong Si, J. Sweedler, 2021, Protein Engineering.

Role of Conserved Glycine in Zinc-dependent Medium Chain Dehydrogenase/Reductase Superfamily*

Huimin Zhao, M. Jeya, Jung-Kul Lee, 2012, The Journal of Biological Chemistry.

Mechanistic investigation of a highly active phosphite dehydrogenase mutant and its application for NADPH regeneration

Huimin Zhao, W. A. van der Donk, Ryan D. Woodyer, 2005, The FEBS journal.

Identification and Characterization of Citrus Peel Uronic Acid Oxidase

Yee Ling Tan, Huimin Zhao, E. Ang, 2020, Chembiochem : a European journal of chemical biology.

Structure and engineering of L-arabinitol 4-dehydrogenase from Neurospora crassa.

Huimin Zhao, S. Nair, R. Sullivan, 2010, Journal of molecular biology.

Synthetic biology advances and applications in the biotechnology industry: a perspective

Huimin Zhao, Ramon Gonzalez, Yvonne Y. Chen, 2018, Journal of Industrial Microbiology & Biotechnology.

Crystal structures of phosphite dehydrogenase provide insights into nicotinamide cofactor regeneration.

Nikhil U. Nair, Huimin Zhao, Y. Zou, 2012, Biochemistry.

Regeneration of Cofactors for Enzyme Biocatalysis

Huimin Zhao, Ryan Woodyer, Huimin Zhao, 2004 .

Recent developments in pyridine nucleotide regeneration.

Huimin Zhao, Wilfred A van der Donk, Huimin Zhao, 2003, Current opinion in biotechnology.

Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis

Huimin Zhao, J. Obbard, E. Ang, 2007, The FEBS journal.

Engineering of Enzymes for Selective Catalysis

Nikhil U. Nair, Huimin Zhao, C. Denard, 2010 .

Directed Evolution of a Thermostable Phosphite Dehydrogenase for NAD(P)H Regeneration

Huimin Zhao, Huimin Zhao, Ryan D. Woodyer, 2005, Applied and Environmental Microbiology.

Biosynthesis of 2-Hydroxyethylphosphonate, an Unexpected Intermediate Common to Multiple Phosphonate Biosynthetic Pathways*

Benjamin T. Circello, Huimin Zhao, W. Metcalf, 2008, Journal of Biological Chemistry.

Improving and repurposing biocatalysts via directed evolution.

Huimin Zhao, Carl A Denard, Hengqian Ren, 2015, Current opinion in chemical biology.

Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution.

Huimin Zhao, Zhi Li, Weng Lin Tang, 2010, Chemical communications.

Reconstitution and Characterization of Aminopyrrolnitrin Oxygenase, a Rieske N-Oxygenase That Catalyzes Unusual Arylamine Oxidation*

Huimin Zhao, M. Simurdiak, Jungkul Lee, 2005, Journal of Biological Chemistry.

Industrial Applications of Enzymes as Catalysts

Nikhil U. Nair, Weng Lin Tang, Huimin Zhao, 2010 .

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

Huimin Zhao, Huimin Zhao, R. Cobb, 2014, ACS synthetic biology.

Recent advances in combinatorial biosynthesis for drug discovery

Huimin Zhao, Huihua Sun, E. Ang, 2015, Drug design, development and therapy.

DNA assembler: a synthetic biology tool for characterizing and engineering natural product gene clusters.

Huimin Zhao, Zengyi Shao, Huimin Zhao, 2012, Methods in enzymology.

Precise Regulation of Cas9-Mediated Genome Engineering by Anti-CRISPR-Based Inducible CRISPR Controllers.

Huimin Zhao, Teresa A. Martin, I. Tasan, 2021, ACS synthetic biology.

Mechanistic studies on the flavin:NADH reductase (PrnF) from Pseudomonas fluorescens involved in arylamine oxygenation.

Huimin Zhao, Jung-Kul Lee, R. Singh, 2012, Bioorganic & medicinal chemistry letters.

Identification and Characterization of the Flavin:NADH Reductase (PrnF) Involved in a Novel Two-Component Arylamine Oxygenase

Huimin Zhao, Jung-Kul Lee, 2007, Journal of bacteriology.

Huimin Zhao Oxygenase a Novel Two-Component Arylamine Flavin : NADH Reductase ( PrnF ) Involved in Identification and Characterization of the

Huimin Zhao, Jung-Kul Lee, 2007 .

CmlI is an N-oxygenase in the biosynthesis of chloramphenicol.

Huimin Zhao, Emmanuel Chanco, Haige Lu, 2012, Tetrahedron.

Recent developments in the application of P450 based biocatalysts.

Huimin Zhao, E. Ang, Yifeng Wei, 2018, Current opinion in chemical biology.

Orthogonal Fatty Acid Biosynthetic Pathway Improves Fatty Acid Ethyl Ester Production in Saccharomyces cerevisiae.

Huimin Zhao, Mohammad HamediRad, Huimin Zhao, 2015, ACS synthetic biology.

Characterization of the N-oxygenase AurF from Streptomyces thioletus.

Huimin Zhao, Y. Choi, N. Sun, 2014, Bioorganic & medicinal chemistry.

High‐throughput Screening Methods for Oxidoreductases

Huimin Zhao, Ryan D. Woodyer, Tyler W. Johannes, 2008 .

Directed Evolution Tools in Bioproduct and Bioprocess Development

Huimin Zhao, Wilfred Chen, Huimin Zhao, 2007 .

Activation of silent biosynthetic gene clusters using transcription factor decoys

Huimin Zhao, S. Dong, Fang Guo, 2018, Nature Chemical Biology.

Breaking the silence: new strategies for discovering novel natural products.

Huimin Zhao, Hengqian Ren, Bin Wang, 2017, Current opinion in biotechnology.

Microbial synthesis of triacetic acid lactone

Huimin Zhao, Zengyi Shao, Dongming Xie, 2006, Biotechnology and bioengineering.

Engineering biological systems using automated biofoundries.

Huimin Zhao, Shekhar Mishra, Ran Chao, 2017, Metabolic engineering.

Drug Discovery and Development by Combinatorial Biosynthesis

Huimin Zhao, C. Denard, Matthew A. DeSieno, 2011 .

Cell surface display of functional human MHC class II proteins: yeast display versus insect cell display.

Huimin Zhao, K. Wucherpfennig, D. Sethi, 2011, Protein engineering, design & selection : PEDS.

Biosystems Design by Machine Learning.

Huimin Zhao, Ismini Lourentzou, Chengxiang Zhai, 2020, ACS synthetic biology.

Directed evolution of soluble single-chain human class II MHC molecules.

Huimin Zhao, O. Esteban, 2004, Journal of molecular biology.

Manipulating Natural Product Biosynthetic Pathways via DNA Assembler

Huimin Zhao, Zengyi Shao, Huimin Zhao, 2014, Current protocols in chemical biology.

Metabolic engineering of Saccharomyces cerevisiae to produce 1-hexadecanol from xylose

Huimin Zhao, Xueyang Feng, Jiayuan Sheng, 2016, Microbial Cell Factories.

Metabolic engineering of Saccharomyces cerevisiae to improve 1-hexadecanol production.

Huimin Zhao, Xueyang Feng, Jiazhang Lian, 2015, Metabolic engineering.

Recent advances in biosynthesis of fatty acids derived products in Saccharomyces cerevisiae via enhanced supply of precursor metabolites

Huimin Zhao, Jiazhang Lian, Huimin Zhao, 2015, Journal of Industrial Microbiology & Biotechnology.

Engineering microbial factories for synthesis of value-added products

Huimin Zhao, Zengyi Shao, Jing Du, 2011, Journal of Industrial Microbiology & Biotechnology.

An extended bacterial reductive pyrimidine degradation pathway that enables nitrogen release from β-alanine

Huimin Zhao, E. Ang, Q. Lu, 2019, The Journal of Biological Chemistry.

A gene cluster for taurine sulfur assimilation in an anaerobic human gut bacterium.

Huimin Zhao, E. Ang, Yiling Hu, 2019, The Biochemical journal.

Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense.

Huimin Zhao, E. Ang, P. Cao, 2019, The Biochemical journal.

Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination

Huimin Zhao, S. Tabatabaei, Behnam Enghiad, 2021, Nature Communications.

Characterization of Cas proteins for CRISPR‐Cas editing in streptomycetes

Mingzi M. Zhang, Shawn Hoon, Huimin Zhao, 2019, Biotechnology and bioengineering.

Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes

Mingzi M. Zhang, Shawn Hoon, Huimin Zhao, 2019, bioRxiv.

Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

Huimin Zhao, Jiazhang Lian, Mohammad HamediRad, 2018, Biotechnology journal.

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

Huimin Zhao, Jiazhang Lian, Mohammad HamediRad, 2017, Nature Communications.

Targeting Specificity of the CRISPR/Cas9 System.

Huimin Zhao, Ipek Tasan, Huimin Zhao, 2017, ACS synthetic biology.

Targeting Speci fi city of the CRISPR / Cas 9 System

Huimin Zhao, I. Tasan, Ipek Tasan, 2017 .

CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

Huimin Zhao, Mingzi M. Zhang, Ee Lui Ang, 2017, Nature chemical biology.

Orthogonal control of endogenous gene expression in mammalian cells using synthetic ligands

Huimin Zhao, M. McLachlan, Jing Liang, 2013, Biotechnology and bioengineering.

CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging of endogenous loci

Huimin Zhao, A. Belmont, Yuchuan Wang, 2017, bioRxiv.

TALEN outperforms Cas9 in editing heterochromatin target sites

Huimin Zhao, P. Selvin, Yuchuan Wang, 2021, Nature Communications.

Programmable DNA-Guided Artificial Restriction Enzymes.

Huimin Zhao, Behnam Enghiad, Huimin Zhao, 2017, ACS synthetic biology.

Recent advances in targeted genome engineering in mammalian systems

Huimin Zhao, Zhanar Abil, Huimin Zhao, 2012, Biotechnology journal.

Fully Automated One-Step Synthesis of Single-Transcript TALEN Pairs Using a Biological Foundry.

Jing Liang, Huimin Zhao, Ran Chao, 2017, ACS synthetic biology.

Engineering Of Therapeutic Proteins

Huimin Zhao, Fei Wen, Sheryl B. Rubin-pitel, 2009 .

in Saccharomyces cerevisiae Saccharification and Ethanol Fermentation Minicellulosome for Simultaneous Engineered Pentafunctional

Huimin Zhao, Tong Si, E. Ang, 2014 .

Directed evolution of homing endonuclease I-SceI with altered sequence specificity.

Zhilei Chen, Huimin Zhao, N. Sun, 2009, Protein engineering, design & selection : PEDS.

High‐throughput Screening Methods Developed for Oxidoreductases

Huimin Zhao, Ryan D. Woodyer, Tyler W. Johannes, 2006 .

PlasmidMaker is a versatile, automated, and high throughput end-to-end platform for plasmid construction

Huimin Zhao, Chengyou Shi, Behnam Enghiad, 2022, Nature Communications.

DNA assembly techniques for next-generation combinatorial biosynthesis of natural products

Huimin Zhao, J. C. Ning, Huimin Zhao, 2014, Journal of Industrial Microbiology & Biotechnology.

Promoter-proximal CTCF-binding promotes long-range-enhancer dependent gene activation

Jesse R. Dixon, B. Ren, D. Gorkin, 2021, Nature structural & molecular biology.

RNAi-Assisted Genome Evolution (RAGE) in Saccharomyces cerevisiae.

Huimin Zhao, Tong Si, 2016, Methods in molecular biology.

Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

Jing Liang, Huimin Zhao, Pu Xue, 2018, Nature Biotechnology.

A Scalable Epitope Tagging Approach for High Throughput ChIP-Seq Analysis.

Huimin Zhao, Bing Ren, Yanxiao Zhang, 2017, ACS synthetic biology.

Recent advances in biocatalyst development in the pharmaceutical industry

Huimin Zhao, Mingzi M. Zhang, Xiaoyun Su, 2013 .

Synthetic Biology for Therapeutic Applications

Huimin Zhao, Zhanar Abil, Huimin Zhao, 2014, Molecular pharmaceutics.

ECNet is an evolutionary context-integrated deep learning framework for protein engineering

Wesley Wei Qian, Jian Peng, Yang Liu, 2021, Nature Communications.

Recent advances in natural product discovery.

Huimin Zhao, Yunzi Luo, Ryan E Cobb, 2014, Current opinion in biotechnology.

Investigating host dependence of xylose utilization in recombinant Saccharomyces cerevisiae strains using RNA-seq analysis

Huimin Zhao, Xueyang Feng, Huimin Zhao, 2013, Biotechnology for Biofuels.

Characterization of the flavin monooxygenase involved in biosynthesis of the antimalarial FR-900098.

Huimin Zhao, S. Nair, R. Cobb, 2019, Organic & biomolecular chemistry.

Structure-guided design and biosynthesis of a novel FR-900098 analogue as a potent Plasmodium falciparum 1-deoxy-D-xylulose-5-phosphate reductoisomerase (Dxr) inhibitor.

Huimin Zhao, S. Nair, R. Cobb, 2015, Chemical communications.

Rapid identification of CD4+ T-cell epitopes using yeast displaying pathogen-derived peptide library.

Huimin Zhao, Fei Wen, O. Esteban, 2008, Journal of immunological methods.

Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains.

Huimin Zhao, Nikhil U. Nair, Nikhil U Nair, 2014, Metabolic engineering.

Outrunning Nature: Directed Evolution of Superior Biocatalysts

Huimin Zhao, Wilfred Chen, Ryan Woodyer, 2004 .

Cloning and characterization of a panel of mitochondrial targeting sequences for compartmentalization engineering in Saccharomyces cerevisiae

Huimin Zhao, J. Lian, Zhinan Xu, 2021, Biotechnology and bioengineering.

Protein engineering in designing tailored enzymes and microorganisms for biofuels production.

Huimin Zhao, Nikhil U. Nair, Nikhil U Nair, 2009, Current opinion in biotechnology.

A ferredoxin-dependent dihydropyrimidine dehydrogenase in Clostridium chromiireducens

Huimin Zhao, E. Ang, Yan Zhang, 2020, Bioscience reports.

A Pathway for Degradation of Uracil to Acetyl Coenzyme A in Bacillus megaterium

Huimin Zhao, E. Ang, Dazhi Liu, 2020, Applied and Environmental Microbiology.

Alginate Lyases from Alginate-Degrading Vibrio splendidus 12B01 Are Endolytic

Huimin Zhao, Christopher V. Rao, C. V. Rao, 2015, Applied and Environmental Microbiology.

High-Throughput Screening or Selection Methods for Evolutionary Enzyme Engineering

Ee Lui Ang, Huimin Zhao, Shuobo Shi, 2016 .

Design and engineering of intracellular‐metabolite‐sensing/regulation gene circuits in Saccharomyces cerevisiae

Meng Wang, Huimin Zhao, M. Wang, 2016, Biotechnology and bioengineering.

Synthetic biology: putting synthesis into biology

Huimin Zhao, Yunzi Luo, Huimin Zhao, 2010, Wiley interdisciplinary reviews. Systems biology and medicine.

A mass spectrometry‐based high‐throughput screening method for engineering fatty acid synthases with improved production of medium‐chain fatty acids

Huimin Zhao, Tong Si, J. Sweedler, 2020, Biotechnology and bioengineering.

Towards a fully automated algorithm driven platform for biosystems design

Saurabh Sinha, Huimin Zhao, Jiazhang Lian, 2019, Nature Communications.

Engineering reprogrammable RNA-binding proteins for study and manipulation of the transcriptome.

Huimin Zhao, Zhanar Abil, 2015, Molecular bioSystems.

Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA

Huimin Zhao, Zhanar Abil, C. Denard, 2014, Journal of biological engineering.

Functional Reconstitution of a Pyruvate Dehydrogenase in the Cytosol of Saccharomyces cerevisiae through Lipoylation Machinery Engineering.

Huimin Zhao, Jiazhang Lian, Huimin Zhao, 2016, ACS synthetic biology.

Comparative Biochemical Characterization of Three Exolytic Oligoalginate Lyases from Vibrio splendidus Reveals Complementary Substrate Scope, Temperature, and pH Adaptations

Huimin Zhao, Jung-Kul Lee, M. Polz, 2014, Applied and Environmental Microbiology.

Recent advances in the bioremediation of persistent organic pollutants via biomolecular engineering

Jeffrey Philip Obbard, Huimin Zhao, Ee Lui Ang, 2005 .

SunnyTALEN: A second‐generation TALEN system for human genome editing

Huimin Zhao, Zehua Bao, Huimin Zhao, 2014, Biotechnology and bioengineering.

A single-chain TALEN architecture for genome engineering.

Huimin Zhao, N. Sun, 2014, Molecular bioSystems.

Further improvement of phosphite dehydrogenase thermostability by saturation mutagenesis

Huimin Zhao, Huimin Zhao, Tyler W Johannes, 2008, Biotechnology and bioengineering.

Building biological foundries for next-generation synthetic biology

Huimin Zhao, Yongbo Yuan, Ran Chao, 2015, Science China Life Sciences.

Crystal Structure and Computational Analyses Provide Insights into the Catalytic Mechanism of 2,4-Diacetylphloroglucinol Hydrolase PhlG from Pseudomonas fluorescens*

Huimin Zhao, Cong-Zhao Zhou, Y. Teng, 2009, The Journal of Biological Chemistry.

CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

Huimin Zhao, Justin L. Ungerer, H. Pakrasi, 2016, Microbial Cell Factories.

Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification.

Huimin Zhao, Fei Wen, Mason R. Smith, 2019, Methods in molecular biology.

Construction and screening of an antigen-derived peptide library displayed on yeast cell surface for CD4+ T cell epitope identification.

Huimin Zhao, Fei Wen, Huimin Zhao, 2013, Methods in molecular biology.

Cellulose to Ethanol Saccharification and Fermentation of Minicellulosomes for Simultaneous Yeast Surface Display of Trifunctional

Huimin Zhao, Jie Sun, Fei Wen, 2014 .

Pathway engineering as an enabling synthetic biology tool

Huimin Zhao, Huimin Zhao, Dawn T. Eriksen, 2013 .

Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold.

Huimin Zhao, Chun Li, Zhanar Abil, 2013, ACS synthetic biology.

Investigating xylose metabolism in recombinant Saccharomyces cerevisiae via 13C metabolic flux analysis

Huimin Zhao, Xueyang Feng, Huimin Zhao, 2013, Microbial Cell Factories.

Directed evolution of estrogen receptor proteins with altered ligand-binding specificities.

Huimin Zhao, Carsten A. Vock, Huimin Zhao, 2009, Protein engineering, design & selection : PEDS.

Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces

Huimin Zhao, E. Takano, Behnam Enghiad, 2020, Journal of Industrial Microbiology & Biotechnology.

Biosynthetic engineering of the antifungal, anti-MRSA auroramycin

Mingzi M. Zhang, Huimin Zhao, E. Ang, 2019, bioRxiv.

Directed evolution of aniline dioxygenase for enhanced bioremediation of aromatic amines

Huimin Zhao, J. Obbard, E. Ang, 2009, Applied Microbiology and Biotechnology.

Use of genome-editing tools to treat sickle cell disease

Huimin Zhao, I. Tasan, Surbhi Jain, 2016, Human Genetics.

Deciphering the late biosynthetic steps of antimalarial compound FR-900098.

N. Kelleher, B. Griffin, Huimin Zhao, 2010, Chemistry & biology.

Recent advances in biocatalysis by directed enzyme evolution.

Huimin Zhao, Huimin Zhao, Sheryl B. Rubin-pitel, 2006, Combinatorial chemistry & high throughput screening.

3 Directed Enzyme Evolution and High-Throughput Screening

Huimin Zhao, R. Sullivan, M. McLachlan, 2009 .

Directed Evolution: Novel and Improved Enzymes

Huimin Zhao, M. McLachlan, Fei Wen, 2008 .

Evolutionary Methods for Protein Engineering

Huimin Zhao, Wenjuan Zha, 2004 .

In vivo biosensors: mechanisms, development, and applications

Huimin Zhao, Ee Lui Ang, Shuobo Shi, 2018, Journal of Industrial Microbiology & Biotechnology.

Expanding the Potential of Mammalian Genome Engineering via Targeted DNA Integration.

Huimin Zhao, Meng Zhang, I. Tasan, 2021, ACS synthetic biology.

Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity.

Huimin Zhao, Huimin Zhao, Wenjuan Zha, 2008, Molecular bioSystems.

Characterization of the Substrate Specificity of PhlD, a Type III Polyketide Synthase from Pseudomonas fluorescens*

Huimin Zhao, Sheryl B. Rubin-pitel, Wenjuan Zha, 2006, Journal of Biological Chemistry.

Biosynthesis of phloroglucinol.

Huimin Zhao, Mo Xian, M. Xian, 2005, Journal of the American Chemical Society.

Natural Product Discovery Top-Down Approaches Bottom-Up Approaches

Huimin Zhao, Yunzi Luo, Ryan E Cobb, 2014 .

Genome mining unveils a class of ribosomal peptides with two amino termini

Huimin Zhao, Hengqian Ren, D. Mitchell, 2023, bioRxiv.

Pathway Design, Engineering, and Optimization.

Huimin Zhao, Mohammad HamediRad, Eva Garcia-Ruiz, 2016, Advances in biochemical engineering/biotechnology.

Two-Color Imaging of Non-repetitive Endogenous Loci in Human Cells.

Huimin Zhao, Shekhar Mishra, Behnam Enghiad, 2020, ACS synthetic biology.

Profiling of Microbial Colonies for High-Throughput Engineering of Multistep Enzymatic Reactions via Optically Guided Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Bin Li, Huimin Zhao, Troy J Comi, 2017, Journal of the American Chemical Society.

Rational pathway engineering of type I fatty acid synthase allows the biosynthesis of triacetic acid lactone from D-glucose in vivo.

Huimin Zhao, Zengyi Shao, Huimin Zhao, 2004, Journal of the American Chemical Society.

Engineering oleaginous yeast Rhodotorula toruloides for overproduction of fatty acid ethyl esters

Huimin Zhao, S. Shi, Jie Peng, 2021, Biotechnology for Biofuels.

Engineering Oleaginous Yeast Rhodosporidium Toruloides for Overproduction of Fatty Acid Ethyl Esters

Huimin Zhao, S. Shi, Jie Peng, 2020 .

Characterization and Engineering of the Adenylation Domain of a NRPS-Like Protein: A Potential Biocatalyst for Aldehyde Generation

M. Wang, Huimin Zhao, 2014, ACS catalysis.

Family shuffling with single-stranded DNA.

Huimin Zhao, Wenjuan Zha, Tongbo Zhu, 2003, Methods in molecular biology.

Rapid Discovery of Glycocins through Pathway Refactoring in Escherichia coli.

Huimin Zhao, Hengqian Ren, Wilfred A van der Donk, 2018, ACS chemical biology.

Discovery and characterization of novel d-xylose-specific transporters from Neurospora crassa and Pichia stipitis.

Huimin Zhao, Huimin Zhao, Sijin Li, 2010, Molecular bioSystems.

Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler.

Huimin Zhao, Yunzi Luo, Zengyi Shao, 2011, Molecular bioSystems.

Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis.

Huimin Zhao, Y. Choi, Zengyi Shao, 2010, Molecular bioSystems.

Further biochemical studies on aminopyrrolnitrin oxygenase (PrnD).

Huimin Zhao, Jung-Kul Lee, M. Tiwari, 2011, Bioorganic & medicinal chemistry letters.

Minihemicellulosome Strains Displaying an Engineered Recombinant Saccharomyces cerevisiae Direct Conversion of Xylan to Ethanol

Huimin Zhao, Tong Si, Jian‐He Xu, 2012 .

A highly efficient and sensitive screening method for trans-activation activity of estrogen receptors.

Zhilei Chen, Huimin Zhao, 2003, Gene.

Highly Efficient Single-Pot Scarless Golden Gate Assembly.

Huimin Zhao, Jiazhang Lian, Mohammad HamediRad, 2019, ACS synthetic biology.

Rapid creation of a novel protein function by in vitro coevolution.

Zhilei Chen, Huimin Zhao, 2005, Journal of molecular biology.

Directed Evolution of Human Estrogen Receptor Variants with Significantly Enhanced Androgen Specificity and Affinity*

B. Katzenellenbogen, Zhilei Chen, Huimin Zhao, 2004, Journal of Biological Chemistry.

Aryl-aldehyde formation in fungal polyketides: discovery and characterization of a distinct biosynthetic mechanism.

M. Wang, Huimin Zhao, Mirko Beissner, 2014, Chemistry & biology.

Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering

Huimin Zhao, Jing Du, Matthew A. DeSieno, 2011 .

Directed evolution of xylose specific transporters to facilitate glucose‐xylose co‐utilization

M. Wang, Huimin Zhao, Chenzhao Yu, 2015, Biotechnology and bioengineering.

In situ assembly of antifouling/bacterial silver nanoparticle-hydrogel composites with controlled particle release and matrix softening.

Dong Hyun Kim, Huimin Zhao, H. Kong, 2015, ACS applied materials & interfaces.

Engineered Pentafunctional Minicellulosome for Simultaneous Saccharification and Ethanol Fermentation in Saccharomyces cerevisiae

Huimin Zhao, Ee Lui Ang, Youyun Liang, 2014, Applied and Environmental Microbiology.

A brief overview of synthetic biology research programs and roadmap studies in the United States

Huimin Zhao, Tong Si, Huimin Zhao, 2016, Synthetic and systems biotechnology.

Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria

Huimin Zhao, E. Ang, Z. Yuchi, 2019, Nature Communications.

DNA punch cards for storing data on native DNA sequences via enzymatic nicking

Alvaro G. Hernandez, D. Soloveichik, J. Leburton, 2020, Nature Communications.

Orthogonal Genetic Regulation in Human Cells Using Chemically Induced CRISPR/Cas9 Activators.

Huimin Zhao, Zehua Bao, Huimin Zhao, 2017, ACS synthetic biology.

Strain Development by Whole-Cell Directed Evolution

Huimin Zhao, Tong Si, J. Lian, 2017 .

A widespread pathway for substitution of adenine by diaminopurine in phage genomes

Huimin Zhao, Suwen Zhao, E. Ang, 2021, Science.

Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae

Huimin Zhao, Ee Lui Ang, Tong Si, 2016, Scientific Reports.

Inducible Control of mRNA Transport Using Reprogrammable RNA-Binding Proteins

C. Hoogenraad, Huimin Zhao, Zhanar Abil, 2017, ACS synthetic biology.

Evolutionary context-integrated deep sequence modeling for protein engineering

Yang Liu, Huimin Zhao, Jian Peng, 2020, bioRxiv.

Directed evolution of novel protein functions.

Huimin Zhao, Huimin Zhao, 2007, Biotechnology and bioengineering.

Mutagenic Inverted Repeats Assisted Genome Engineering (MIRAGE) in Saccharomyces cerevisiae: deletion of gal7.

Nikhil U. Nair, Huimin Zhao, 2012, Methods in molecular biology.

A new fluorescence complementation biosensor for detection of estrogenic compounds

Huimin Zhao, Huimin Zhao, J. Katzenellenbogen, 2011, Biotechnology and bioengineering.

Evolution in Reverse: Engineering a D‐Xylose‐Specific Xylose Reductase

Huimin Zhao, Nikhil U. Nair, Huimin Zhao, 2008, Chembiochem : a European journal of chemical biology.

Genome-wide identification of natural RNA aptamers in prokaryotes and eukaryotes

N. Nagarajan, Huimin Zhao, X. Lim, 2018, Nature Communications.

Identification of an important motif that controls the activity and specificity of sugar transporters

M. Wang, Huimin Zhao, Chenzhao Yu, 2016, Biotechnology and bioengineering.

Production of long chain alcohols and alkanes upon coexpression of an acyl-ACP reductase and aldehyde-deformylating oxygenase with a bacterial type-I fatty acid synthase in E. coli.

Huimin Zhao, J. Lian, J. Shanklin, 2015, Molecular bioSystems.

Mechanistic studies on the conversion of arylamines into arylnitro compounds by aminopyrrolnitrin oxygenase: identification of intermediates and kinetic studies.

Huimin Zhao, Jungkul Lee, 2006, Angewandte Chemie.

Mechanistically Diverse Pathways for Sulfoquinovose Degradation in Bacteria

Huimin Zhao, E. Ang, Yan Zhang, 2021, ACS Catalysis.

A transaldolase-dependent sulfoglycolysis pathway in Bacillus megaterium DSM 1804.

Huimin Zhao, E. Ang, Yan Zhou, 2020, Biochemical and biophysical research communications.

Two radical-dependent mechanisms for anaerobic degradation of the globally abundant organosulfur compound dihydroxypropanesulfonate

Huimin Zhao, E. Ang, P. Cao, 2020, Proceedings of the National Academy of Sciences.

A two-plasmid bacterial selection system for characterization and engineering of homing endonucleases.

Huimin Zhao, N. Sun, 2014, Methods in molecular biology.

Flexible and Versatile Strategy for the Construction of Large Biochemical Pathways.

Huimin Zhao, Yongbo Yuan, Erik Andersen, 2016, ACS synthetic biology.

Identification of novel metabolic engineering targets for S-adenosyl-L-Methionine production in Saccharomyces cerevisiae via genome-scale engineering.

Huimin Zhao, J. Lian, Zhinan Xu, 2021, Metabolic engineering.

Mutagenic inverted repeat assisted genome engineering (MIRAGE)

Huimin Zhao, Nikhil U. Nair, Huimin Zhao, 2008, Nucleic acids research.

Industrial biotechnology: Tools and applications

Huimin Zhao, Weng Lin Tang, Huimin Zhao, 2009, Biotechnology journal.

Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution.

Huimin Zhao, Tong Si, P. Xue, 2020, Methods in enzymology.

Design and application of a kinetic model of lipid metabolism in Saccharomyces cerevisiae.

Michael J. Volk, Huimin Zhao, Shekhar Mishra, 2022, Metabolic Engineering.

Mining and application of constitutive promoters from Rhodosporidium toruloides

Huimin Zhao, S. Shi, Zhenzhen Bai, 2023, AMB Express.

TALE proteins search DNA using a rotationally decoupled mechanism.

Huimin Zhao, Luke Cuculis, Zhanar Abil, 2016, Nature chemical biology.

Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca.

Huimin Zhao, E. Ang, Z. Yuchi, 2019, The Biochemical journal.

Heterologous Expression, Purification, and Characterization of a Highly Active Xylose Reductase from Neurospora crassa

Huimin Zhao, Ryan Woodyer, Huimin Zhao, 2005, Applied and Environmental Microbiology.

Directed evolution of phloroglucinol synthase PhlD with increased stability for phloroglucinol production

Huimin Zhao, Jung-Kul Lee, G. Rao, 2013, Applied Microbiology and Biotechnology.

Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole

Huimin Zhao, Suwen Zhao, E. Ang, 2018, Nature Communications.

Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole

Huimin Zhao, Ee Lui Ang, Yan Zhang, 2018, Nature Communications.

A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data

Hoang V. Dinh, C. V. Rao, C. Maranas, 2019, bioRxiv.

Directed evolution of the nonribosomal peptide synthetase AdmK generates new andrimid derivatives in vivo.

N. Kelleher, B. Evans, Huimin Zhao, 2011, Chemistry & biology.

DNA punch cards for storing data on native DNA sequences via enzymatic nicking

Olgica Milenkovic, Huimin Zhao, David Soloveichik, 2020, Nature Communications.

Protein engineering of gene switches and scissors for human gene therapy

Huimin Zhao, 2008 .

Challenges and opportunities in synthetic biology for chemical engineers.

Huimin Zhao, Jung-Kul Lee, Yunzi Luo, 2013, Chemical engineering science.

Recent advances in combinatorial biosynthesis for drug discovery

Huimin Zhao, Huihua Sun, E. Ang, 2015, Drug design, development and therapy.

Biomolecular Engineering

Huimin Zhao, Ee Lui Ang, Zengyi Shao, 2005 .

Engineering microbial hosts for production of bacterial natural products.

Mingzi M. Zhang, Huimin Zhao, E. Ang, 2016, Natural product reports (Print).

A Pathway for Isethionate Dissimilation in Bacillus krulwichiae

Huimin Zhao, E. Ang, Yiling Hu, 2019, Applied and Environmental Microbiology.

A pH-indicator-based screen for hydrolytic haloalkane dehalogenase.

Huimin Zhao, 2003, Methods in molecular biology.

Recent advances in natural product discovery.

Huimin Zhao, R. Cobb, Yunzi Luo, 2014, Current opinion in biotechnology.

Directed evolution of proteins for biomedical application

Huimin Zhao, Zhilei Chen, Zhilei Chen, 2005 .

Standardization for natural product synthetic biology.

Huimin Zhao, M. Medema, 2016, Natural product reports.

Cloning and characterization of a galactitol 2-dehydrogenase from Rhizobium legumenosarum and its application in D-tagatose production.

Huimin Zhao, Jung-Kul Lee, Ranjitha Singh, 2014, Enzyme and microbial technology.

Directed Evolution of Replication-Competent Double-Stranded DNA Bacteriophage toward New Host Specificity.

Huimin Zhao, E. Ang, Jing Liang, 2022, ACS synthetic biology.

Highly Efficient Single-Pot Scarless Golden Gate Assembly.

Huimin Zhao, J. Lian, Ran Chao, 2019, ACS synthetic biology.

Use of genome-editing tools to treat sickle cell disease

Huimin Zhao, I. Tasan, Surbhi Jain, 2016, Human Genetics.

New N-Acetyltransferase Fold in the Structure and Mechanism of the Phosphonate Biosynthetic Enzyme FrbF*

Huimin Zhao, S. Nair, R. Cobb, 2011, The Journal of Biological Chemistry.

Combined and Iterative Use of Computational Design and Directed Evolution for Protein-Ligand Binding Design.

Meng Wang, Huimin Zhao, M. Wang, 2016, Methods in molecular biology.

Delta Integration CRISPR-Cas (Di-CRISPR) in Saccharomyces cerevisiae.

Huimin Zhao, E. Ang, S. Shi, 2019, Methods in molecular biology.

Construction and engineering of large biochemical pathways via DNA assembler.

Huimin Zhao, Zengyi Shao, Huimin Zhao, 2013, Methods in molecular biology.

DNA assembler method for construction of zeaxanthin-producing strains of Saccharomyces cerevisiae.

Huimin Zhao, Yunzi Luo, Zengyi Shao, 2012, Methods in molecular biology.

Further biochemical studies on aminopyrrolnitrin oxygenase (PrnD).

Huimin Zhao, Jung-Kul Lee, M. Tiwari, 2011, Bioorganic & medicinal chemistry letters.