S. Akanuma

发表

Combinatorial mutagenesis to restrict amino acid usage in an enzyme to a reduced set

S. Akanuma, T. Kigawa, S. Yokoyama, 2002, Proceedings of the National Academy of Sciences of the United States of America.

Comprehensive mutagenesis to identify amino acid residues contributing to the difference in thermostability between two originally thermostable ancestral proteins

S. Akanuma, A. Yamagishi, Minako Yamaguchi, 2021, PloS one.

Phylogeny-based design of a B-subunit of DNA gyrase and its ATPase domain using a small set of homologous amino acid sequences.

S. Akanuma, A. Yamagishi, S. Yokobori, 2011, Journal of molecular biology.

Experimental evidence for the thermophilicity of ancestral life

S. Akanuma, K. Miyazono, M. Tanokura, 2013, Proceedings of the National Academy of Sciences.

Reconstructed ancestral enzymes suggest long-term cooling of Earth’s photic zone since the Archean

Satoshi Akanuma, Akihiko Yamagishi, Shin-ichi Yokobori, 2017, Proceedings of the National Academy of Sciences.

A detailed unfolding pathway of a (β/α)8‐barrel protein as studied by molecular dynamics simulations

S. Akanuma, A. Yamagishi, K. Kitamura, 2004 .

Selection of a platinum-binding sequence in a loop of a four-helix bundle protein.

S. Akanuma, A. Yamagishi, Sota Yagi, 2017, Journal of bioscience and bioengineering.

Coarse-Grained Molecular Dynamics Simulation of IPMDH Proteins

S. Akanuma, H. Yamada, A. Yamagishi, 2014 .

Effect of polar side chains at position 172 on thermal stability of 3‐isopropylmalate dehydrogenase from Thermus thermophilus

S. Akanuma, T. Oshima, A. Yamagishi, 1997, FEBS letters.

Establishment of mesophilic-like catalytic properties in a thermophilic enzyme without affecting its thermal stability

S. Akanuma, A. Yamagishi, S. Yokobori, 2019, Scientific Reports.

Constructing protein nano-fiber and estimation of the electronic state around metal ions

S. Akanuma, S. Yokojima, H. Yamada, 2012 .

STRUCTURE OF 3-ISOPROPYLMALATE DEHYDROGENASE

S. Akanuma, T. Oshima, N. Tanaka, 1997 .

Ancestral Sequence Reconstruction of the Ribosomal Protein uS8 and Reduction of Amino Acid Usage to a Smaller Alphabet

S. Akanuma, Fangzheng Zhao, 2022, Journal of Molecular Evolution.

Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method

S. Akanuma, T. Oshima, A. Yamagishi, 1996, Journal of bacteriology.

Partial purification and characterization of polyphenoloxidase from culinary-medicinal Royal Sun mushroom (the Himematsutake), Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae).

S. Akanuma, A. Yamagishi, N. Ohno, 2011, International journal of medicinal mushrooms.

Comparative Genomics of Thermophilic Bacteria and Archaea

S. Akanuma, A. Yamagishi, S. Yokobori, 2013 .

Preparation of Phi29 DNA Polymerase Free of Amplifiable DNA Using Ethidium Monoazide, an Ultraviolet-Free Light-Emitting Diode Lamp and Trehalose

Takayoshi Kobayashi, S. Akanuma, T. Ohtani, 2014, PloS one.

Mimicking the evolution of a thermally stable monomeric four-helix bundle by fusion of four identical single-helix peptides.

S. Akanuma, A. Yamagishi, Naoki Umeda, 2010, Journal of biochemistry.

Methods for reducing nonspecific interaction in antibody-antigen assay via atomic force microscopy.

S. Akanuma, T. Ohtani, S. Sugiyama, 2008, Analytical biochemistry.

Ancestral sequence reconstruction produces thermally stable enzymes with mesophilic enzyme-like catalytic properties

S. Akanuma, Ryutaro Furukawa, K. Yamazaki, 2020, Scientific Reports.

Characterization of Reconstructed Ancestral Proteins Suggests a Change in Temperature of the Ancient Biosphere

Satoshi Akanuma, S. Akanuma, 2017, Life.

N-terminal Hydrophobic Amino Acids of Activating Transcription Factor 5 (ATF5) Protein Confer Interleukin 1β (IL-1β)-induced Stabilization*

S. Akanuma, M. Kojima, Shigeru Takahashi, 2013, The Journal of Biological Chemistry.

The Common Ancestor of All Modern Life

S. Akanuma, 2019, Astrobiology.

Birth of Archaeal Cells: Molecular Phylogenetic Analyses of G1P Dehydrogenase, G3P Dehydrogenases, and Glycerol Kinase Suggest Derived Features of Archaeal Membranes Having G1P Polar Lipids

S. Akanuma, A. Yamagishi, S. Yokobori, 2016, Archaea.

Comprehensive reduction of amino acid set in a protein suggests the importance of prebiotic amino acids for stable proteins

S. Akanuma, A. Yamagishi, Masami Shimada, 2018, Scientific Reports.

Serial increase in the thermal stability of 3‐isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution

S. Akanuma, T. Oshima, A. Yamagishi, 1998, Protein science : a publication of the Protein Society.

Further improvement of the thermal stability of a partially stabilized Bacillus subtilis 3-isopropylmalate dehydrogenase variant by random and site-directed mutagenesis.

S. Akanuma, T. Oshima, A. Yamagishi, 1999, European journal of biochemistry.

Robustness of predictions of extremely thermally stable proteins in ancient organisms

Satoshi Akanuma, Akihiko Yamagishi, Shin-ichi Yokobori, 2015, Evolution; international journal of organic evolution.

Experimental evidence for the existence of a stable half-barrel subdomain in the (beta/alpha)8-barrel fold.

S. Akanuma, A. Yamagishi, 2008, Journal of molecular biology.

A Strategy for Designing Thermostable Enzymes by Reconstructing Ancestral Sequences Possessed by Ancient Life

S. Akanuma, A. Yamagishi, 2016 .

Characterization of a thermostable mutant of Agaricus brasiliensis laccase created by phylogeny-based design.

S. Akanuma, A. Yamagishi, N. Ohno, 2017, Journal of bioscience and bioengineering.

Roles for the two N‐terminal (β/α) modules in the folding of a (β/α)8‐barrel protein as studied by fragmentation analysis

S. Akanuma, A. Yamagishi, 2011, Proteins.

Epistasis effects of multiple ancestral-consensus amino acid substitutions on the thermal stability of glycerol kinase from Cellulomonas sp. NT3060.

S. Akanuma, A. Yamagishi, S. Yokobori, 2016, Journal of bioscience and bioengineering.

De novo design of protein-protein interactions through modification of inter-molecular helix-helix interface residues.

S. Akanuma, A. Yamagishi, Sota Yagi, 2016, Biochimica et biophysica acta.

Reconstruction and Characterization of Thermally Stable and Catalytically Active Proteins Comprising an Alphabet of ~ 13 Amino Acids

S. Akanuma, M. Kimura, 2020, Journal of Molecular Evolution.

RECONSTRUCTED ANCESTRAL ENZYMES SUGGEST THAT EARTH'S PHOTIC-ZONE TEMPERATURE MARKEDLY DECREASED OVER GEOLOGIC TIME

S. Akanuma, A. Yamagishi, S. Yokobori, 2016 .

Substitutions of coenzyme-binding, nonpolar residues improve the low-temperature activity of thermophilic dehydrogenases.

S. Akanuma, A. Yamagishi, Sayaka Hayashi, 2011, Biochemistry.

Random mutagenesis improves the low-temperature activity of the tetrameric 3-isopropylmalate dehydrogenase from the hyperthermophile Sulfolobus tokodaii.

S. Akanuma, A. Yamagishi, Mayumi Uno, 2008, Protein engineering, design & selection : PEDS.

Recombination-Deficient Mutants of an Extreme Thermophile, Thermus thermophilus

S. Akanuma, T. Oshima, A. Yamagishi, 1993, Applied and environmental microbiology.

Identification and characterization of key substructures involved in the early folding events of a (β/α)8-barrel protein as studied by experimental and computational methods

S. Akanuma, A. Yamagishi, 2005 .

Activation of macrophages by a laccase-polymerized polyphenol is dependent on phosphorylation of Rac1.

S. Akanuma, Y. Adachi, N. Ohno, 2018, Biochemical and Biophysical Research Communications - BBRC.

Cloning and characterization of laccase DNA from the Royal Sun medicinal mushroom, Agaricus brasiliensis (higher Basidiomycetes).

S. Akanuma, A. Yamagishi, N. Ohno, 2014, International journal of medicinal mushrooms.

Reconstruction and Characterization of Thermally Stable and Catalytically Active Proteins Comprising an Alphabet of ~ 13 Amino Acids

S. Akanuma, M. Kimura, 2020, Journal of Molecular Evolution.

Characterization of the low-temperature activity of Sulfolobus tokodaii glucose-1-dehydrogenase mutants.

S. Akanuma, A. Yamagishi, T. Sugii, 2014, Journal of bioscience and bioengineering.

Addition of negatively charged residues can reverse the decrease in the solubility of an acidic protein caused by an artificially introduced non-polar surface patch.

S. Akanuma, A. Yamagishi, Sota Yagi, 2014, Biochimica et biophysica acta.

Creation of artificial protein–protein interactions using α-helices as interfaces

S. Akanuma, A. Yamagishi, Sota Yagi, 2017, Biophysical Reviews.

Crystal structures of 3-isopropylmalate dehydrogenases with mutations at the C-terminus: crystallographic analyses of structure-stability relationships.

S. Akanuma, Takao Sato, N. Tanaka, 2000, Protein engineering.

Spontaneous tandem sequence duplications reverse the thermal stability of carboxyl-terminal modified 3-isopropylmalate dehydrogenase

S. Akanuma, T. Oshima, A. Yamagishi, 1996, Journal of bacteriology.

A mutation at the interface between domains causes rearrangement of domains in 3-isopropylmalate dehydrogenase.

S. Akanuma, T. Oshima, N. Tanaka, 1997, Protein engineering.

Designing the Binding Surface of Proteins to Construct Nano-fibers

S. Akanuma, A. Yamagishi, M. Takasu, 2012 .

Identification and characterization of key substructures involved in the early folding events of a (beta/alpha)8-barrel protein as studied by experimental and computational methods.

S. Akanuma, A. Yamagishi, 2005, Journal of molecular biology.

Comparative analysis of reconstructed ancestral proteins with their extant counterparts suggests primitive life had an alkaline habitat

S. Akanuma, Takahiro Sasamoto, Takayuki Fujikawa, 2024, Scientific reports.