G. Milligan

B. Hudson, Marijn T. M. van Jaarsveld, D. Peeper, 2017, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

Distinct Phosphorylation Clusters Determine the Signaling Outcome of Free Fatty Acid Receptor 4/G Protein–Coupled Receptor 120

Ashley M. Miller, B. Hudson, G. Milligan, 2016, Molecular Pharmacology.

Distinct phosphorylation clusters determine the signalling outcome of the free fatty acid receptor FFA 4 / GPR 120

Ashley M. Miller, B. Hudson, G. Milligan, 2016 .

Indomethacin Treatment Prevents High Fat Diet-induced Obesity and Insulin Resistance but Not Glucose Intolerance in C57BL/6J Mice*

K. Kristiansen, L. Madsen, B. Hudson, 2014, The Journal of Biological Chemistry.

Succinct synthesis of saturated hydroxy fatty acids and in vitro evaluation of all hydroxylauric acids on FFA1, FFA4 and GPR84.

G. Milligan, T. Ulven, J. Dunlop, 2017, MedChemComm.

Effects of dexamethasone on G protein levels and adenylyl cyclase activity in rat vascular smooth muscle cells.

S. Tawil, J. Connell, G. Milligan, 1992, Journal of molecular endocrinology.

Roundabout 1 exists predominantly as a basal dimeric complex and this is unaffected by binding of the ligand Slit2.

G. Milligan, R. Ward, J. Pediani, 2014, Biochemical Journal.

Real-time monitoring of redox changes in the mammalian endoplasmic reticulum

G. Milligan, J. Pediani, M. van Lith, 2011, Journal of Cell Science.

Differences in the Signaling Pathways of α1A- and α1B-Adrenoceptors Are Related to Different Endosomal Targeting

G. Milligan, J. Pediani, M. Pérez-Aso, 2013, PloS one.

Regulation of cellular Gsα levels and basal adenylyl cyclase activity by expression of the β2-adrenoceptor in neuroblastoma cell lines

G. Milligan, Gun-Do Kim, I. Mullaney, 1995, Molecular and Cellular Biochemistry.

The stoichiometry of expression of protein components of the stimulatory adenylyl cyclase cascade and the regulation of information transfer.

G. Milligan, 1996, Cellular signalling.

Overexpression of Gsα in NG108‐15, neuroblastoma X glioma cells: effects on receptor regulation of the stimulatory adenylyl cyclase cascade

G. Milligan, I. Mullaney, I. Craig Carr, 1996 .

Discovery of 9-cyclopropylethynyl-2-((S)-1-[1,4]dioxan-2-ylmethoxy)-6,7-dihydropyrimido[6,1-a]isoquinolin-4-one (GLPG1205), a Unique GPR84 Negative Allosteric Modulator Undergoing Evaluation in a Phase II Clinical Trial.

L. Nelles, G. Milligan, S. Dupont, 2020, Journal of medicinal chemistry.

Ligand regulation of green fluorescent protein‐tagged forms of the human β1‐ and β2‐adrenoceptors; comparisons with the unmodified receptors

G. Milligan, A. J. McLean, 2000 .

G-Protein-Coupled Receptor 35 Mediates Human Saphenous Vein Vascular Smooth Muscle Cell Migration and Endothelial Cell Proliferation

G. Milligan, C. Delles, S. Nicklin, 2016, Journal of Vascular Research.

Insulin activates glycerol-3-phosphate acyltransferase (de novo phosphatidic acid synthesis) through a phospholipid-derived mediator. Apparent involvement of Gi alpha and activation of a phospholipase C.

G. Milligan, R. Farese, M. Standaert, 1990, Biochemistry.

Mapping binding sites for the PDE4D5 cAMP-specific phosphodiesterase to the N- and C-domains of beta-arrestin using spot-immobilized peptide arrays.

G. Milligan, G. Baillie, A. Dunlop, 2007, The Biochemical journal.

Why are there so many adrenoceptor subtypes?

G. Milligan, P. Svoboda, C. M. Brown, 1994, Biochemical pharmacology.

Wortmannin-sensitive Activation of p70 by Endogenous and Heterologously Expressed G-coupled Receptors (*)

G. Milligan, N. Anderson, M. Wilson, 1996, The Journal of Biological Chemistry.

beta-Arrestin 1 and Galphaq/11 coordinately activate RhoA and stress fiber formation following receptor stimulation.

J. Violin, R. Lefkowitz, G. Milligan, 2005, The Journal of biological chemistry.

Mutations of beta-arrestin 2 that limit self-association also interfere with interactions with the beta2-adrenoceptor and the ERK1/2 MAPKs: implications for beta2-adrenoceptor signalling via the ERK1/2 MAPKs.

W. Kolch, G. Milligan, Tianrui Xu, 2008, The Biochemical journal.

Efficacy of inverse agonists in cells overexpressing a constitutively active β2‐adrenoceptor and type II adenylyl cyclase

G. Milligan, P. Stevens, 1998, British journal of pharmacology.

The D2-dopamine receptor of anterior pituitary is functionally associated with a pertussis toxin-sensitive guanine nucleotide binding protein.

M. Caron, G. Milligan, J. Benovic, 1987, The Journal of biological chemistry.

Quantitative stoichiometry of the proteins of the stimulatory arm of the adenylyl cyclase cascade in neuroblastoma x glioma hybrid, NG108-15 cells.

G. Milligan, G. D. Kim, E. Adie, 1994, European journal of biochemistry.

Guanine nucleotide binding proteins in neuroblastoma x glioma hybrid, NG108-15, cells. Regulation of expression and function.

G. Milligan, S. McClue, F. McKenzie, 1990, The International journal of biochemistry.

The α2 B adrenergic receptor of undifferentiated neuroblastoma × glioma hybrid NG108‐15 cells, interacts directly with the guanine nucleotide binding protein, Gi2

G. Milligan, S. McClue, Steven J. McClue, 1990, FEBS letters.

Elevated levels of the guanine nucleotide binding protein, Go, are associated with differentiation of neuroblastoma × glioma hybrid cells

G. Milligan, I. Mullaney, Ian Mullaney, 1989, FEBS letters.

Agonist-induced endocytosis and recycling of the gonadotropin-releasing hormone receptor: effect of beta-arrestin on internalization kinetics.

G. Milligan, M. Vrecl, A. Hanyaloglu, 1998, Molecular endocrinology.

Stimulation of p70S6 kinase phosphorylation by the A2A adenosine receptor in primary human endothelial cells and in CHO cells heterologously expressing the receptor

G. Milligan, N. Anderson, M. Freissmuth, 1998 .

Ligand‐induced internalization of the orexin OX1 and cannabinoid CB1 receptors assessed via N‐terminal SNAP and CLIP‐tagging

G. Milligan, R. Ward, J. Pediani, 2011, British journal of pharmacology.

Selectivity and functional consequences of interactions of family A G protein‐coupled receptors with neurochondrin and periplakin

G. Milligan, R. Ward, Laura Jenkins, 2009, Journal of neurochemistry.

Multiple Roles for the C-terminal Tail of the Chemokine Scavenger D6*

N. Isaacs, G. Milligan, S. Milasta, 2008, Journal of Biological Chemistry.

Analysis of the C-terminal tail of the rat thyrotropin-releasing hormone receptor-1 in interactions and cointernalization with beta-arrestin 1-green fluorescent protein.

S. Wilson, G. Milligan, N. Evans, 2001, Molecular pharmacology.

Functional analysis of a human A1 adenosine receptor/green fluorescent protein/Gi1α fusion protein following stable expression in CHO cells

S. Rees, T. Palmer, G. Milligan, 1999, FEBS letters.

A Key Serine for the GTPase-Activating Protein Function of Regulator of G Protein Signaling Proteins Is Not a General Target for 14-3-3 Interactions

G. Milligan, R. Ward, 2005, Molecular Pharmacology.

The role of palmitoylation of the guanine nucleotide binding protein G11 alpha in defining interaction with the plasma membrane.

G. Milligan, A. Magee, M. Parenti, 1995, The Biochemical journal.

A novel N-terminal motif for palmitoylation of G-protein alpha subunits.

G. Milligan, M. Vigano, A. Magee, 1993, The Biochemical journal.

Agonist-induced transfer of the alpha subunits of the guanine-nucleotide-binding regulatory proteins Gq and G11 and of muscarinic m1 acetylcholine receptors from plasma membranes to a light-vesicular membrane fraction.

G. Milligan, P. Svoboda, 1994, European journal of biochemistry.

Agonist regulation of cellular G protein levels and distribution: mechanisms and functional implications.

G. Milligan, G. Milligan, 1993, Trends in pharmacological sciences.

Angiotensin1‐9 antagonises pro‐hypertrophic signalling in cardiomyocytes via the angiotensin type 2 receptor

G. Milligan, C. Haggerty, S. Nicklin, 2011, The Journal of physiology.

Development and Characterization of a Potent Free Fatty Acid Receptor 1 (FFA1) Fluorescent Tracer.

B. Hudson, G. Milligan, E. Christiansen, 2016, Journal of medicinal chemistry.

The Action and Mode of Binding of Thiazolidinedione Ligands at Free Fatty Acid Receptor 1*♦

Leigh A. Stoddart, G. Milligan, N. J. Smith, 2009, The Journal of Biological Chemistry.

Structural Characterization of Agonist Binding to Protease-Activated Receptor 2 through Mutagenesis and Computational Modeling.

G. Milligan, Johan Johansson, Jens Carlsson, 2018, ACS pharmacology & translational science.

Chemokine receptor CXCR4 oligomerization is disrupted selectively by the antagonist ligand IT1t

V. Raicu, G. Milligan, T. Handel, 2020, Journal of Biological Chemistry.

G-Protein-Coupled Receptor Dimers

G. Giovanni, G. Milligan, K. Herrick‐Davis, 2017, The Receptors.

Generation and analysis of constitutively active and physically destabilized mutants of the human beta(1)-adrenoceptor.

G. Milligan, F. Zeng, D. Behan, 2002, Molecular pharmacology.

Ligand regulation of green fluorescent protein-tagged forms of the human beta(1)- and beta(2)-adrenoceptors; comparisons with the unmodified receptors.

G. Milligan, A. J. McLean, 2000, British journal of pharmacology.

Measurement of agonist efficacy using an α2A‐adrenoceptor‐Gi1α fusion protein

G. Milligan, A. Wise, I. C. Carr, 1997 .

Measurement of agonist efficacy using an alpha2A-adrenoceptor-Gi1alpha fusion protein.

G. Milligan, A. Wise, I. C. Carr, 1997, FEBS letters.

Chemical inhibition of myristoylation of the G-protein Gi1 alpha by 2-hydroxymyristate does not interfere with its palmitoylation or membrane association. Evidence that palmitoylation, but not myristoylation, regulates membrane attachment.

G. Milligan, F. Galbiati, A. Magee, 1996, The Biochemical journal.

Robustness of G Proteins in Alzheimer's Disease: An Immunoblot Study

J. Mcculloch, G. Milligan, B. Ross, 1991, Journal of neurochemistry.

How do G-proteins stay at the plasma membrane?

G. Milligan, M. Grassie, 1997, Essays in biochemistry.

Prostaglandin E1-mediated, cyclic AMP-independent, down-regulation of Gs alpha in neuroblastoma x glioma hybrid cells.

G. Milligan, F. McKenzie, 1990, The Journal of biological chemistry.

Stimulation of high-affinity GTPase activity and cholera toxin-catalysed [32P]ADP-ribosylation of Gi by lysophosphatidic acid (LPA) in wild-type and alpha 2C10 adrenoceptor-transfected Rat 1 fibroblasts.

G. Milligan, C. Carr, M. Grassie, 1994, The Biochemical journal.

Novel Role for Proteinase-activated Receptor 2 (PAR2) in Membrane Trafficking of Proteinase-activated Receptor 4 (PAR4)*

S. Mundell, G. Milligan, J. Pediani, 2012, The Journal of Biological Chemistry.

Analysis of Pertussis Toxin-Sensitive Receptor: G-Protein Interactions in Native Porcine Endothelial Cells

C. Lowenstein, G. Milligan, N. Flavahan, 1995 .

Cannabinoid receptor type 1 protects against age-related osteoporosis by regulating osteoblast and adipocyte differentiation in marrow stromal cells.

G. Milligan, S. Ralston, D. Baker, 2009, Cell metabolism.

Multiple pertussis toxin‐sensitive G‐proteins can couple receptors to GIRK channels in rat sympathetic neurons when expressed heterologously, but only native Gi‐proteins do so in situ

G. Milligan, P. Delmas, D. Brown, 2001, The European journal of neuroscience.

G protein–coupled receptor modulation with pepducins: moving closer to the clinic

T. Schwartz, G. Milligan, T. Sakmar, 2011, Annals of the New York Academy of Sciences.

Mechanism of Action of G q to Inhibit G (cid:1)(cid:2) Modulation of Ca V 2.2 Calcium Channels: Probed by the Use of Receptor-G (cid:3) Tandems

G. Milligan, A. Dolphin, R. Ward, 2003 .

Muscarinic Acetylcholine Receptors in Neuroblastoma Cells: Lack of Effect of Veratrum Alkaloids on Receptor Number

G. Milligan, P. Strange, Philip G. Strange, 1984, Journal of neurochemistry.

The effect of sodium channel activators on muscarinic receptors of neuroblastoma cells

G. Milligan, P. Strange, Philip G. Strange, 1982, FEBS letters.

Isolation and characterization of a novel human RGS mutant displaying gain-of-function activity.

G. Ladds, G. Milligan, Claire Hill, 2008, Cellular signalling.

G-Protein–Coupled Receptor Mas Is a Physiological Antagonist of the Angiotensin II Type 1 Receptor

P. Sexton, A. Christopoulos, T. Walther, 2005, Circulation.

Chemogenetic Approaches to Explore the Functions of Free Fatty Acid Receptor 2.

G. Milligan, A. Tobin, N. Barki, 2021, Trends in pharmacological sciences.

Identification of novel species-selective agonists of the G-protein-coupled receptor GPR35 that promote recruitment of β-arrestin-2 and activate Gα13.

B. Hudson, M. Loza, J. Brea, 2010, The Biochemical journal.

Agonist activation of transfected human M1 muscarinic acetylcholine receptors in CHO cells results in down-regulation of both the receptor and the alpha subunit of the G-protein Gq.

G. Milligan, N. Buckley, I. Mullaney, 1993, The Biochemical journal.

Functional Homomers and Heteromers of Dopamine D2L and D3 Receptors Co-exist at the Cell Surface

Leigh A. Stoddart, M. Millan, G. Milligan, 2012, The Journal of Biological Chemistry.

Visualizing differences in ligand regulation of wild-type and constitutively active mutant beta(2)-adrenoceptor-green fluorescent protein fusion proteins.

S. Rees, G. Milligan, N. Bevan, 1999, Molecular pharmacology.

Predicting small molecule fluorescent probe localization in living cells using QSAR modeling. 1. Overview and models for probes of structure, properties and function in single cells

G. Milligan, J. Pediani, R. Horobin, 2013, Biotechnic & histochemistry : official publication of the Biological Stain Commission.

Syddansk Universitet Probe-Dependent Negative Allosteric Modulators of the Long-Chain Free Fatty Acid Receptor FFA 4

B. Hudson, G. Milligan, S. Yarwood, 2017 .

Up-regulation of the levels of expression and function of a constitutively active mutant of the hamster alpha1B-adrenoceptor by ligands that act as inverse agonists.

G. Milligan, S. Cotecchia, T. Lee, 1997, The Biochemical journal.

Guanine nucleotide regulation of the pertussis and cholera toxin substrates of rat glioma C6 BU1 cells.

G. Milligan, 1987, Biochimica et biophysica acta.

Reciprocal mutations of highly conserved residues in transmembrane helices 2 and 7 of the α2A-adrenoceptor restore agonist activation of Gi1α

G. Milligan, R. Ward, 2002 .

Histamine receptor H1 is required for TCR-mediated p38 MAPK activation and optimal IFN-gamma production in mice.

J. Zachary, G. Milligan, C. Teuscher, 2007, The Journal of clinical investigation.

An Asp79Asn mutation of the alpha2A-adrenoceptor interferes equally with agonist activation of individual Gialpha-family G protein subtypes.

G. Milligan, R. Ward, 1999, FEBS letters.

Morphine Desensitization, Internalization, and Down-Regulation of the μ Opioid Receptor Is Facilitated by Serotonin 5-Hydroxytryptamine2A Receptor Coactivation

G. Milligan, J. López-Giménez, M. Vilaró, 2008, Molecular Pharmacology.

Autoimmune Disease-Associated Alleles Affect Histamine Receptor H1 Protein Trafficking and Cell Surface Expression

Cory, Teuscher, G. Milligan, 2008 .

δ‐Opioid receptors exhibit high efficiency when activating trimeric G proteins in membrane domains

J. Novotný, G. Milligan, P. Svoboda, 2003, Journal of neurochemistry.

Thyrotropin‐releasing hormone‐induced depletion of Gqα/G11α proteins from detergent‐insensitive membrane domains

J. Novotný, G. Milligan, P. Svoboda, 1999 .

Opioid receptors and their interacting proteins

G. Milligan, 2007, NeuroMolecular Medicine.

Purification of heterotrimeric GTP-binding proteins from brain: identification of a novel form of Go.

G. Milligan, P. Backlund, A. Spiegel, 1988, Biochemistry.

Tissue-specific regulation of GTP-binding protein and muscarinic acetylcholine receptor levels during cardiac development.

G. Milligan, P. Gierschik, A. Spiegel, 1987, Biochemistry.

Analysis of endogenous S1P and LPA receptor expression in CHO-K1 cells.

P. Slocombe, G. Milligan, G. Hutchinson, 2005, Gene.

The insulin receptor tyrosyl kinase phosphorylates holomeric forms of the guanine nucleotide regulatory proteins Gi and Go

K. Siddle, R. O’Brien, G. Milligan, 1987, FEBS letters.

Mechanism of Gonadotrophin Releasing Hormone Receptor Mediated Regulation of G-Proteins in Clonal Pituitary Gonadotrophs

B. H. Shah, G. Milligan, D. MacEwan, 1996 .

Cellular localization and pharmacological characterization of functioning alpha-1 adrenoceptors by fluorescent ligand binding and image analysis reveals identical binding properties of clustered and diffuse populations of receptors.

G. Milligan, J. McGrath, C. Daly, 1998, The Journal of pharmacology and experimental therapeutics.

Cellular Localization and Pharmacological Characterization of Functioning Alpha-1 Adrenoceptors by Fluorescent Ligand Binding and Image Analysis Reveals Identical Binding Properties of Clustered and Diffuse Populations of Receptors 1

G. Milligan, J. McGrath, C. Daly, 1998 .

Modulation of SF1 Neuron Activity Coordinately Regulates Both Feeding Behavior and Associated Emotional States

M. Ungless, G. Milligan, Mark A. Smith, 2017, Cell reports.

Strategies to identify ligands for orphan G-protein-coupled receptors.

G. Milligan, 2001, Biochemical Society transactions.

GPCR homo-oligomerization

G. Milligan, R. Ward, Sara Marsango, 2019, Current opinion in cell biology.

Guanine nucleotide regulatory proteins in the spontaneously hypertensive rat.

J. Connell, G. Milligan, C. J. Clark, 1993, Hypertension.

Guanine nucleotide regulatory protein levels and function in spontaneously hypertensive rat vascular smooth-muscle cells.

J. Connell, G. Milligan, C. J. Clark, 1992, Biochimica et biophysica acta.

The Dually Acylated NH2-terminal Domain of Gi1α Is Sufficient to Target a Green Fluorescent Protein Reporter to Caveolin-enriched Plasma Membrane Domains

G. Milligan, M. Lisanti, D. Lublin, 1999, Journal of Biological Chemistry.

A cysteine-3 to serine mutation of the G-protein Gi1 alpha abrogates functional activation by the alpha 2A-adrenoceptor but not interactions with the beta gamma complex.

S. Rees, G. Milligan, M. Parenti, 1997, Biochemistry.

Orthologue selectivity and ligand bias: translating the pharmacology of GPR35.

G. Milligan, 2011, Trends in pharmacological sciences.

Agonist-induced phosphorylation of orthologues of the orphan receptor GPR35 functions as an activation sensor

B. Hudson, G. Milligan, A. Tobin, 2022, The Journal of biological chemistry.

Dimerization of α1-adrenoceptors

G. Milligan, J. Pediani, M. Fidock, 2004 .

The sphingosine-1-phosphate receptor-1 antagonist, W146, causes early and short-lasting peripheral blood lymphopenia in mice.

G. Milligan, G. Tarrasón, S. Kunkel, 2011, International immunopharmacology.

Ligand Rescue of Constitutively Active Mutant Receptors

G. Milligan, D. Ramsay, A. J. McLean, 2002, Neurosignals.

Visualization of Agonist-induced Association and Trafficking of Green Fluorescent Protein-tagged Forms of Both β-Arrestin-1 and the Thyrotropin-releasing Hormone Receptor-1*

Shelagh Wilson, G. Milligan, C. Krasel, 1999, The Journal of Biological Chemistry.

Real Time Visualization of Agonist-mediated Redistribution and Internalization of a Green Fluorescent Protein-tagged Form of the Thyrotropin-releasing Hormone Receptor*

G. Milligan, G. Gould, T. Drmota, 1998, The Journal of Biological Chemistry.

Interactions of the alpha2A-adrenoceptor with multiple Gi-family G-proteins: studies with pertussis toxin-resistant G-protein mutants.

S. Rees, G. Milligan, A. Wise, 1997, The Biochemical journal.

Analysis of inverse agonism at the delta opioid receptor after expression in Rat 1 fibroblasts.

G. Milligan, I. Mullaney, I. C. Carr, 1996, The Biochemical journal.

Agonist-dependent, cholera toxin-catalyzed ADP-ribosylation of pertussis toxin-sensitive G-proteins following transfection of the human alpha 2-C10 adrenergic receptor into rat 1 fibroblasts. Evidence for the direct interaction of a single receptor with two pertussis toxin-sensitive G-proteins, Gi2

G. Milligan, G. Gould, C. Carr, 1991, The Journal of biological chemistry.

Design , Synthesis , and Evaluation of a Diazirine Photoaffinity Probe for Ligand-Based Receptor Capture Targeting G Protein – Coupled Receptors s

G. Milligan, A. Tobin, R. Liskamp, 2019 .

Multiple defects occur in the guanine nucleotide regulatory protein system in liver plasma membranes of obese (fa/fa) but not lean (Fa/Fa) Zucker rats: loss of functional Gi and abnormal Gs function.

G. Milligan, M. Houslay, A. Wilson, 1989, Cellular signalling.

Title Inferring Signaling Pathway Topologies from MultiplePerturbation Measurements of Specific Biochemical Species

M. Girolami, W. Kolch, G. Milligan, 2018 .

Localization of the succinate receptor in the distal nephron and its signaling in polarized MDCK cells.

G. Milligan, P. Deen, R. Fenton, 2009, Kidney international.

Beta-arrestin-dependent spontaneous alpha1a-adrenoceptor endocytosis causes intracellular transportation of alpha-blockers via recycling compartments.

G. Milligan, J. Pediani, J. McGrath, 2005, Molecular pharmacology.

CypHer 5: a generic approach for measuring the activation and trafficking of G protein-coupled receptors in live cells.

G. Milligan, S. Game, K. Hadingham, 2003, Assay and drug development technologies.

Measurement of Agonist-Dependent and -Independent Signal Initiation of α1b-Adrenoceptor Mutants by Direct Analysis of Guanine Nucleotide Exchange on the G Protein Gα11

G. Milligan, J. J. Carrillo, P. Stevens, 2002, Journal of Pharmacology and Experimental Therapeutics.

Principles: extending the utility of [35S]GTP gamma S binding assays.

G. Milligan, 2003, Trends in pharmacological sciences.

Gi down-regulation as a mechanism for heterologous desensitization in adipocytes.

G. Milligan, A. Green, S. Dobias, 1992, The Journal of biological chemistry.

Agonist control of G-protein levels.

G. Milligan, A. Green, 1991, Trends in pharmacological sciences.

The regulator of G protein signaling RGS4 selectively enhances alpha 2A-adreoreceptor stimulation of the GTPase activity of Go1alpha and Gi2alpha.

G. Milligan, K. Druey, A. Cavalli, 2000, The Journal of biological chemistry.

Syddansk Universitet Fatty acid 16 : 4 ( n-3 ) stimulates a GPR 120-induced signaling cascade in splenic macrophages to promote chemotherapy resistance

B. Hudson, D. Peeper, G. Milligan, 2017 .

Ligand specific up-regulation of a Renilla reniformis luciferase-tagged, structurally unstable muscarinic M3 chimeric G protein-coupled receptor.

G. Milligan, F. Zeng, D. Behan, 2003, Molecular pharmacology.

Selective Interactions between Helix VIII of the Human μ-Opioid Receptors and the C Terminus of Periplakin Disrupt G Protein Activation*

C. Scorer, G. Milligan, Julia H. M. White, 2003, Journal of Biological Chemistry.

Resolution of inverse agonist-induced up-regulation from constitutive activity of mutants of the alpha(1b)-adrenoceptor.

S. Rees, G. Milligan, N. Bevan, 2000, Molecular pharmacology.

Persistent activation of the alpha subunit of Gs promotes its removal from the plasma membrane.

G. Milligan, C. Unson, G. Milligan, 1989, The Biochemical journal.

Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis

C. Malone, G. Milligan, R. Ward, 2012, The EMBO journal.

Treatment of intact hepatocytes with either the phorbol ester TPA or glucagon elicits the phosphorylation and functional inactivation of the inhibitory guanine nucleotide regulatory protein Gi

G. Milligan, M. Houslay, N. Pyne, 1989, FEBS letters.

Heterodimerisation of G protein-coupled receptors: implications for drug design and ligand screening

G. Milligan, L. S. del Burgo, 2010, Expert opinion on drug discovery.

Quantitative analysis of a cysteine351glycine mutation in the G protein Gi1α: effect on α2A‐adrenoceptor‐Gi1α fusion protein activation

S. Rees, G. Milligan, V. Jackson, 1998 .

New Aspects of G-Protein-Coupled Receptor Signalling and Regulation

G. Milligan, 1998, Trends in Endocrinology & Metabolism.

The Translation Factor eIF3a Regulates the Extracellular Signal regulated 1 Kinase (ERK) Pathway 2

G. Milligan, Tianrui Xu, M. Houslay, 2017 .

eIF3a interferes with Raf-1 activation and eIF3a downregulation by siRNA enhances ERK

G. Milligan, Tianrui Xu, M. Houslay, 2011 .

Uncovering the pharmacology of the G protein-coupled receptor GPR 40 : high apparent constitutive activity in [ 35 S ] GTP γ S binding studies reflects binding of an endogenous agonist

Leigh A. Stoddart, G. Milligan, Andrew J. Brown, 2006 .

Loss of activation of Gs but not Gi following expression of an α2A‐adrenoceptor‐Gi1α fusion protein

G. Milligan, M. Sautel, 1998 .

Is Promiscuity of G Protein Interaction an Issue in the Classification of Receptors?

G. Milligan, 1997, Annals of the New York Academy of Sciences.

Molecular interaction of the human alpha 2-C10-adrenergic receptor, when expressed in Rat-1 fibroblasts, with multiple pertussis toxin-sensitive guanine nucleotide-binding proteins: studies with site-directed antisera.

G. Milligan, S. McClue, 1991, Molecular pharmacology.

Targeted Elimination of G Proteins and Arrestins Defines Their Specific Contributions to Both Intensity and Duration of G Protein-coupled Receptor Signaling*

G. Milligan, A. Tobin, A. Inoue, 2016, The Journal of Biological Chemistry.

G-Protein–Coupled Receptor Mas Is a Physiological Antagonist of the Angiotensin II Type 1 Receptor Molecular Cardiology

P. Sexton, A. Christopoulos, G. Milligan, 2005 .

Therapeutic validation of an orphan G protein‐coupled receptor: The case of GPR84

G. Milligan, A. Tobin, Sara Marsango, 2020, British journal of pharmacology.

Abolition of the expression of inhibitory guanine nucleotide regulatory protein Gi activity in diabetes

G. Milligan, M. Houslay, A. Spiegel, 1987, Nature.

G-proteins as mediators of cellular signalling processes

G. Milligan, M. Houslay, 1990 .

Concurrent specific immunological detection of both primate and rodent forms of the guanine nucleotide binding protein G11 alpha following their coexpression.

G. Milligan, G. D. Kim, G. D. Kim, 1994, Biochimica et biophysica acta.

Qualitative and quantitative characterization of the distribution of G-protein α subunits in mammals

G. Milligan, 1993 .

Widespread distribution of Gqα/Gllα detected immunologically by an antipeptide antiserum directed against the predicted C‐terminal decapeptide

G. Milligan, S. Arkinstall, M. Wakelam, 1991 .

Activation, cellular redistribution and enhanced degradation of the G proteins Gq and G11 by endogenously expressed and transfected phospholipase C-coupled muscarinic m1 acetylcholine receptors.

G. Milligan, P. Svoboda, M. Caulfield, 1996, Progress in brain research.

Structure–Activity Relationship Studies of Tetrahydroquinolone Free Fatty Acid Receptor 3 Modulators

B. Hudson, G. Milligan, A. Tobin, 2020, Journal of medicinal chemistry.

Development and Characterization of a Fluorescent Tracer for the Free Fatty Acid Receptor 2 (FFA2/GPR43).

B. Hudson, G. Milligan, S. Pandey, 2017, Journal of medicinal chemistry.

Complex Pharmacology of Novel Allosteric Free Fatty Acid 3 Receptor Ligands

B. Hudson, G. Milligan, E. Christiansen, 2014, Molecular Pharmacology.

A disulfide bonding interaction role for cysteines in the extracellular domain of the thyrotropin-releasing hormone receptor.

G. Milligan, K. Eidne, A. McGregor, 1996, Endocrinology.

The G Protein α Subunit Has a Key Role in Determining the Specificity of Coupling to, but Not the Activation of, G Protein-gated Inwardly Rectifying K+ Channels*

G. Milligan, A. Tinker, J. Leaney, 2000, The Journal of Biological Chemistry.

Measurement of agonist-induced guanine nucleotide turnover by the G-protein Gi1alpha when constrained within an alpha2A-adrenoceptor-Gi1alpha fusion protein.

G. Milligan, A. Wise, I. C. Carr, 1997, The Biochemical journal.

Analysis of the role of receptor number in defining the intrinsic activity and potency of partial agonists in neuroblastoma x glioma hybrid NG108-15 cells transfected to express differing levels of the human beta 2-adrenoceptor.

G. Milligan, D. MacEwan, G. D. Kim, 1995, Molecular pharmacology.

Angiotensin-(1-9) antagonises cardiac remodelling via the angiotensin type 2 receptor

G. Milligan, S. Nicklin, D. Graham, 2013 .

Selective deficiency of guanine nucleotide-binding protein Go in two dopamine-resistant pituitary tumors.

G. Milligan, A. Spiegel, P. Goldsmith, 1988, Endocrinology.

G-protein palmitoylation: regulation and functional significance.

G. Milligan, D. MacEwan, A. Magee, 1995, Biochemical Society transactions.

Phosphorylation of the M1 muscarinic acetylcholine receptor mediates protection in neurodegenerative disease

B. Hudson, G. Milligan, A. Tobin, 2021, bioRxiv.

Diabetes-induced alterations in the expression, functioning and phosphorylation state of the inhibitory guanine nucleotide regulatory protein Gi-2 in hepatocytes.

G. Milligan, M. Houslay, P. Parker, 1990, The Biochemical journal.

Hormonal regulation of Gi2 α-subunit phosphorylation in intact hepatocytes

V. Hruby, G. Milligan, M. Houslay, 1990 .

Delta-opioid-receptor-mediated inhibition of adenylate cyclase is transduced specifically by the guanine-nucleotide-binding protein Gi2.

G. Milligan, F. McKenzie, G. Milligan, 1990, The Biochemical journal.

Diabetes abolishes the GTP-dependent, but not the receptor-dependent inhibitory function of the inhibitory guanine-nucleotide-binding regulatory protein (Gi) on adipocyte adenylate cyclase activity.

G. Milligan, M. Houslay, D. Strassheim, 1990, The Biochemical journal.

Glucagon desensitization of adenylate cyclase and stimulation of inositol phospholipid metabolism does not involve the inhibitory guanine nucleotide regulatory protein Gi, which is inactivated upon challenge of hepatocytes with glucagon.

G. Milligan, M. Houslay, N. Pyne, 1989, The Biochemical journal.

Specific antibodies and the selective inhibitor ICI 118233 demonstrate that the hormonally stimulated 'dense-vesicle' and peripheral-plasma-membrane cyclic AMP phosphodiesterases display distinct tissue distributions in the rat.

G. Milligan, M. Houslay, N. Anderson, 1987, The Biochemical journal.

S18616, a highly potent, spiroimidazoline agonist at alpha(2)-adrenoceptors: I. Receptor profile, antinociceptive and hypothermic actions in comparison with dexmedetomidine and clonidine.

M. Millan, D. Cussac, A. Newman-Tancredi, 2000, The Journal of pharmacology and experimental therapeutics.

Spatial intensity distribution analysis quantifies the extent and regulation of homodimerization of the secretin receptor

G. Milligan, L. Miller, R. Ward, 2017, The Biochemical journal.

1 Spatial Intensity Distribution Analysis Quantifies the Extent and Regulation of Homo-Dimerization of the Secretin Receptor

G. Milligan, L. Miller, R. Ward, 2017 .

The α1b-Adrenoceptor Exists as a Higher-Order Oligomer: Effective Oligomerization Is Required for Receptor Maturation, Surface Delivery, and Function

Graeme Milligan, Meritxell Canals, G. Milligan, 2007, Molecular Pharmacology.

Regulation of Oligomeric Organization of the Serotonin 5-Hydroxytryptamine 2C (5-HT2C) Receptor Observed by Spatial Intensity Distribution Analysis

G. Milligan, R. Ward, J. Pediani, 2015, The Journal of Biological Chemistry.

Identification of a Novel Serotonin / Glutamate Receptor Complex Implicated in Psychosis

Callado, S. Sealfon, T. Yuen, 2009 .

Agonist-induced Internalization of the G Protein G11α and Thyrotropin-releasing Hormone Receptors Proceed on Different Time Scales*

J. Novotný, G. Milligan, Gun-Do Kim, 1998, The Journal of Biological Chemistry.

On-target and off-target effects of novel orthosteric and allosteric activators of GPR84

S. McMahon, G. Milligan, A. Tobin, 2019, Scientific Reports.

G-protein-coupled receptor heterodimers: pharmacology, function and relevance to drug discovery.

Graeme Milligan, G. Milligan, 2006, Drug discovery today.

Co‐expression of mu and delta opioid receptors as receptor‐G protein fusions enhances both mu and delta signalling via distinct mechanisms

G. Milligan, D. Massotte, L. Snook, 2008, Journal of neurochemistry.

GPCR Theme Editorial

G. Milligan, J. McGrath, 2009, British journal of pharmacology.

Chimaeric G alpha proteins: their potential use in drug discovery.

S. Rees, G. Milligan, 1999, Trends in pharmacological sciences.

Periplakin Interferes with G Protein Activation by the Melanin-concentrating Hormone Receptor-1 by Binding to the Proximal Segment of the Receptor C-terminal Tail*

G. Feng, G. Milligan, Julia H. M. White, 2005, Journal of Biological Chemistry.

A general method to quantify ligand-driven oligomerization from fluorescence-based images

V. Raicu, G. Milligan, R. Ward, 2019, Nature Methods.

Stimulus-response coupling in FMLP-stimulated U937 monocytes: effect of differentiation on Gi2 expression.

G. Milligan, J. Creba, F. Mitchell, 1990, Biochimica et biophysica acta.

Differential G‐protein expression during B‐ and T‐cell development

G. Milligan, M. Harnett, W. Harnett, 1997, Immunology.

Discovery of a potent and selective GPR120 agonist.

B. Hudson, G. Milligan, B. Shimpukade, 2012, Journal of medicinal chemistry.

Degradation of G/G Is Accelerated by Agonist Occupancy of , , and Adrenergic Receptors (*)

G. Milligan, D. MacEwan, A. Wise, 1995, The Journal of Biological Chemistry.

Binding to GDP-agarose identifies a novel 60kDa substrate for the insulin receptor tyrosyl kinase in mouse NIH-3T3 cells expressing high concentrations of the human insulin receptor.

K. Siddle, R. O’Brien, G. Milligan, 1989, Biochemical and biophysical research communications.

Analysis of agonist function at fusion proteins between the IP prostanoid receptor and cognate, unnatural and chimaeric G-proteins.

G. Milligan, C. Fong, 1999, The Biochemical journal.

Modulation of SF 1 Neuron Activity Coordinately Regulates Both Feeding Behavior and Associated Emotional States Graphical

M. Ungless, G. Milligan, Mark A. Smith, 2017 .

G-proteins in essential hypertension: a study of human platelet plasma membranes

J. Connell, G. Milligan, M. Houslay, 1993, Journal of hypertension.

Generation and Analysis of Constitutively Active and Physically Destabilized Mutants of the Human (cid:1) 1 -Adrenoceptor

G. Milligan, F. Zeng, D. Behan, 2002 .

Foetal calf serum enhances cholera toxin-catalysed ADP-ribosylation of the pertussis toxin-sensitive guanine nucleotide binding protein, Gi2, in rat glioma C6BU1 cells.

G. Milligan, 1989, Cellular signalling.

Identification of both Gi2 and a novel, immunologically distinct, form of Go in rat myometrial membranes

G. Milligan, S. Harbon, O. Goureau, 1989, FEBS letters.

GTP analogues cause release of the alpha subunit of the GTP binding protein, GO, from the plasma membrane of NG108-15 cells.

G. Milligan, A. Magee, C. Unson, 1988, Biochemical and biophysical research communications.

Differential distribution of signal-transducing G-proteins in retina

C. Gerfen, G. Milligan, P. Gierschik, 1987, Brain Research.

THE USE OF SPECIFIC ANTISERA TO STUDY THE DEVELOPMENTAL REGULATION OF GUANINE NUCLEOTIDE BINDING PROTEINS

G. Milligan, P. Gierschik, A. Spiegel, 1987 .

Antibodies which recognize the C-terminus of the inhibitory guanine-nucleotide-binding protein (Gi) demonstrate that opioid peptides and foetal-calf serum stimulate the high-affinity GTPase activity of two separate pertussis-toxin substrates.

G. Milligan, F. McKenzie, A. Spiegel, 1988, The Biochemical journal.

GTP-binding proteins in brain and neutrophil are tethered to the plasma membrane via their amino termini.

G. Milligan, P. Gierschik, A. Spiegel, 1987, Biochemical and biophysical research communications.

Chemically induced hypothyroidism produces elevated amounts of the alpha subunit of the inhibitory guanine nucleotide binding protein (Gi) and the beta subunit common to all G-proteins.

G. Milligan, A. Spiegel, E. Saggerson, 1987, The Biochemical journal.

Development of opiate receptors and GTP-binding regulatory proteins in neonatal rat brain.

G. Milligan, P. Gierschik, A. Spiegel, 1987, The Journal of biological chemistry.

Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides

J. Falloon, G. Milligan, H. Malech, 1986, FEBS letters.

Immunochemical evidence for a novel pertussis toxin substrate in human neutrophils.

J. Falloon, G. Milligan, M. Pines, 1986, The Journal of biological chemistry.

Changes in the guanine nucleotide‐binding proteins, Gi and go, during differentiation of 3T3‐L1 cells

B. Morrow, G. Milligan, P. Gierschik, 1986, FEBS letters.

The β subunit of the guanine nucleotide regulatory proteins: Identification of its location on two‐dimesional gels of brain tissue and its regional and subcellular distribution in brain

D. Jacobowitz, G. Milligan, P. Gierschik, 1986 .

The inhibitory guanine nucleotide-binding protein (Ni) purified from bovine brain is a high affinity GTPase.

G. Milligan, W. Klee, 1985, The Journal of biological chemistry.

Specificity of interactions of receptors and effectors with GTP-binding proteins in native membranes.

G. Milligan, F. McKenzie, I. Mullaney, 1990, Biochemical Society symposium.

Agonist-mediated tyrosine phosphorylation of isoforms of the shc adapter protein by the delta opioid receptor.

G. Milligan, N. Anderson, I. Mullaney, 1997, Cellular signalling.

The Pharmacology and Function of Receptors for Short-Chain Fatty Acids

G. Milligan, A. Tobin, D. Bolognini, 2016, Molecular Pharmacology.

Chemically engineering ligand selectivity at the free fatty acid receptor 2 based on pharmacological variation between species orthologs

B. Hudson, G. Milligan, E. Kostenis, 2012, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

Selective Orthosteric Free Fatty Acid Receptor 2 (FFA2) Agonists

B. Hudson, G. Milligan, E. Kostenis, 2011, The Journal of Biological Chemistry.

Agonism and allosterism: the pharmacology of the free fatty acid receptors FFA2 and FFA3

Leigh A. Stoddart, G. Milligan, N. J. Smith, 2009, British journal of pharmacology.

Conserved Polar Residues in Transmembrane Domains V, VI, and VII of Free Fatty Acid Receptor 2 and Free Fatty Acid Receptor 3 Are Required for the Binding and Function of Short Chain Fatty Acids*

Leigh A. Stoddart, G. Milligan, Andrew J. Brown, 2008, Journal of Biological Chemistry.

Non-equivalence of Key Positively Charged Residues of the Free Fatty Acid 2 Receptor in the Recognition and Function of Agonist Versus Antagonist Ligands*

B. Hudson, G. Milligan, A. Mackenzie, 2015, The Journal of Biological Chemistry.

Melatonin Receptors Couple Through a Cholera Toxin‐Sensitive Mechanism to Inhibit Cyclic AMP in the Ovine Pituitary

G. Milligan, D. Hazlerigg, P. Morgan, 1995, Journal of neuroendocrinology.

Discovery of a potent and selective free fatty acid receptor 1 agonist with low lipophilicity and high oral bioavailability.

B. Hudson, G. Milligan, E. Kostenis, 2013, Journal of medicinal chemistry.

International Union of Pharmacology. LXXI. Free Fatty Acid Receptors FFA1, -2, and -3: Pharmacology and Pathophysiological Functions

Leigh A. Stoddart, G. Milligan, N. J. Smith, 2008, Pharmacological Reviews.

Principles: extending the utility of [35S]GTP gamma S binding assays.

G. Milligan, 2003, Trends in pharmacological sciences.

The other side of opioid receptor signalling: regulation by protein-protein interaction.

Graeme Milligan, G. Milligan, Z. Georgoussi, 2012, Current drug targets.

Expression of Gsα, Connexin-43, Connexin-26, and EP1, 3, and 4 Receptors in Myometrium of Prelabor Singleton Versus Multiple Gestations and the Effects of Mechanical Stretch and Steroids on Gsα

G. Milligan, S. Lye, T. Teoh, 2002, The Journal of the Society for Gynecologic Investigation: JSGI.

Expression of Gsalpha, connexin-43, connexin-26, and EP1, 3, and 4 receptors in myometrium of prelabor singleton versus multiple gestations and the effects of mechanical stretch and steroids on Gsalpha.

G. Milligan, S. Lye, T. Teoh, 2002, Journal of the Society for Gynecologic Investigation.

The CXCR1 and CXCR2 Receptors Form Constitutive Homo- and Heterodimers Selectively and with Equal Apparent Affinities*

Graeme Milligan, G. Milligan, Shirley Wilson, 2005, Journal of Biological Chemistry.

Constitutive Activity of the Cannabinoid CB1 Receptor Regulates the Function of Co-expressed Mu Opioid Receptors*

Graeme Milligan, Meritxell Canals, G. Milligan, 2008, Journal of Biological Chemistry.

Constitutive activity and inverse agonists of G protein-coupled receptors: a current perspective.

G. Milligan, 2003, Molecular pharmacology.

A Novel Allosteric Activator of Free Fatty Acid 2 Receptor Displays Unique Gi-functional Bias*

Ashley M. Miller, G. Milligan, A. Tobin, 2016, The Journal of Biological Chemistry.

The experimental power of FR900359 to study Gq-regulated biological processes

H. Dohlman, C. Müller, M. Hesse, 2015, Nature Communications.

International Union of Basic and Clinical Pharmacology. LXVII. Recommendations for the Recognition and Nomenclature of G Protein-Coupled Receptor Heteromultimers

Michel Bouvier, Lakshmi Devi, Jonathan A. Javitch, 2007, Pharmacological Reviews.

Dimers of Class A G Protein-coupled Receptors Function via Agonist-mediated Trans-activation of Associated G Proteins*

Graeme Milligan, G. Milligan, J. Pediani, 2003, Journal of Biological Chemistry.

Receptor for the Pain Modulatory Neuropeptides FF and AF Is an Orphan G Protein-coupled Receptor*

J. Chambers, H. Sarau, R. Ames, 2000, The Journal of Biological Chemistry.

Minireview: The Effects of Species Ortholog and SNP Variation on Receptors for Free Fatty Acids

B. Hudson, G. Milligan, H. Murdoch, 2013, Molecular endocrinology.

Design, Synthesis, and Evaluation of a Diazirine Photoaffinity Probe for Ligand-Based Receptor Capture Targeting G Protein–Coupled Receptors

G. Milligan, A. Tobin, R. Liskamp, 2018, Molecular Pharmacology.

The emerging pharmacology and function of GPR35 in the nervous system

G. Milligan, A. Mackenzie, 2017, Neuropharmacology.

Alterations in G-protein expression and the hormonal regulation of adenylate cyclase in the adipocytes of obese (fa/fa) Zucker rats.

T. Palmer, G. Milligan, M. Houslay, 1991, The Biochemical journal.

Applications of bioluminescence- and fluorescence resonance energy transfer to drug discovery at G protein-coupled receptors.

Graeme Milligan, G. Milligan, 2004, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

Identification of stable opioid receptor Go-protein complexes using GTP-binding protein selective antisera

G. Milligan, C. Zioudrou, Z. Georgoussi, 1995 .

Tailoring cAMP-signalling responses through isoform multiplicity.

G. Milligan, M. Houslay, 1997, Trends in biochemical sciences.

Monitoring Receptor Oligomerization Using Time-resolved Fluorescence Resonance Energy Transfer and Bioluminescence Resonance Energy Transfer

S. Rees, Shelagh Wilson, G. Milligan, 2001, The Journal of Biological Chemistry.

Chemogenetics defines receptor-mediated functions of short chain free fatty acids

B. Hudson, G. Milligan, A. Tobin, 2019, Nature Chemical Biology.

Identification and characterization of small-molecule ligands that maintain pluripotency of human embryonic stem cells.

Graeme Milligan, Andrew H Baker, Joanne C Mountford, 2010, Biochemical Society transactions.

G16 as a universal G protein adapter: implications for agonist screening strategies.

S. Rees, G. Milligan, F. Marshall, 1996, Trends in pharmacological sciences.

Gonadotrophin-releasing hormone receptor agonist-mediated down-regulation of Gq alpha/G11 alpha (pertussis toxin-insensitive) G proteins in alpha T3-1 gonadotroph cells reflects increased G protein turnover but not alterations in mRNA levels.

B. H. Shah, G. Milligan, D. MacEwan, 1995, Proceedings of the National Academy of Sciences of the United States of America.

Distribution and relative levels of expression of the phosphoinositidase-C-linked G-proteins Gqα and G11α: Absence of G11α in human platelets and haemopoietically derived cell lines

G. Milligan, I. Mullaney, J. F. McCALLUM, 1993 .

Guanine-nucleotide-binding proteins expressed in rat white adipose tissue. Identification of both mRNAs and proteins corresponding to Gi1, Gi2 and Gi3.

G. Milligan, M. Houslay, J. Knowler, 1989, The Biochemical journal.

Domain Swapping in the Human Histamine H1 Receptor

Graeme Milligan, Rob Leurs, R. Leurs, 2004, Journal of Pharmacology and Experimental Therapeutics.

Structural and biophysical characterisation of G protein-coupled receptor ligand binding using resonance energy transfer and fluorescent labelling techniques.

G. Milligan, R. Ward, 2014, Biochimica et biophysica acta.

Building a new conceptual framework for receptor heteromers.

Michel Bouvier, Graeme Milligan, Jonathan A Javitch, 2009, Nature chemical biology.

Metabolic and inflammatory functions of short-chain fatty acid receptors

G. Milligan, D. Bolognini, Domonkos Dedeo, 2020, Current Opinion in Endocrine and Metabolic Research.

Engineering a V(2) vasopressin receptor agonist- and regulator of G-protein-signaling-sensitive G protein.

G. Feng, G. Milligan, A. Cavalli, 2002, Analytical biochemistry.

MicroRNAs regulating cell pluripotency and vascular differentiation.

G. Milligan, N. Kane, A. Baker, 2011, Vascular pharmacology.

MicroRNA regulation of endothelial homeostasis and commitment-implications for vascular regeneration strategies using stem cell therapies.

G. Milligan, J. Mountford, A. Baker, 2013, Free radical biology & medicine.

Role of MicroRNAs 99b, 181a, and 181b in the Differentiation of Human Embryonic Stem Cells to Vascular Endothelial Cells

G. Milligan, J. McClure, C. Delles, 2012, Stem cells.

EMBRYONIC STEM CELLS / INDUCED PLURIPOTENT STEM CELLS Role of MicroRNAs 99 b , 181 a , and 181 b in the Differentiation of Human Embryonic Stem Cells to Vascular Endothelial Cells

G. Milligan, J. McClure, C. Delles, 2012 .

Profiling of transcriptional and epigenetic changes during directed endothelial differentiation of human embryonic stem cells identifies FOXA2 as a marker of early mesoderm commitment

G. Milligan, J. McClure, M. McBride, 2013, Stem Cell Research & Therapy.

G-proteins and second messengers in mitogenesis.

G. Milligan, M. Wakelam, 1989, Progress in growth factor research.

Tissue distribution and subcellular location of guanine nucleotide binding proteins: implications for cellular signalling.

G. Milligan, 1989, Cellular signalling.

Antibodies that identify only the active conformation of Gi family G protein α subunits

S. Rees, J. R. Lane, G. Milligan, 2008, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

G Protein Coupling and Ligand Selectivity of the D2L and D3 Dopamine Receptors

Graeme Milligan, Alan Wise, S. Rees, 2008, Journal of Pharmacology and Experimental Therapeutics.

Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia

L. Baki, A. Georgakopoulos, N. Robakis, 2016, Science Signaling.

The Role of miRNA in Stem Cell Pluripotency and Commitment to the Vascular Endothelial Lineage

G. Milligan, N. Kane, K. White, 2012, Microcirculation.

Autoimmune disease-associated histamine receptor H1 alleles exhibit differential protein trafficking and cell surface expression

G. Milligan, C. Teuscher, M. Rincón, 2008, The Journal of Immunology.

Autoimmune Disease-Associated Histamine Receptor H1 Alleles Exhibit Differential Protein Trafficking and Cell Surface Expression1

G. Milligan, C. Teuscher, M. Rincón, 2008, The Journal of Immunology.

ExpressionProtein Trafficking and Cell Surface Alleles Exhibit Differential 1 Receptor H Autoimmune Disease-Associated Histamine

G. Milligan, C. Teuscher, M. Rincón, 2008 .

Erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA) blocks differentiation and maintains the expression of pluripotency markers in human embryonic stem cells.

G. Milligan, G. Baillie, M. Houslay, 2010, The Biochemical journal.

Gut Dysbiosis during Influenza Contributes to Pulmonary Pneumococcal Superinfection through Altered Short-Chain Fatty Acid Production.

G. Milligan, R. Le Goffic, M. Teixeira, 2020, Cell reports.

Lymphocyte trafficking through the blood–brain barrier is dependent on endothelial cell heterotrimeric G‐protein signaling

G. Milligan, P. Adamson, J. Greenwood, 2002, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

Metabolism meets immunity: The role of free fatty acid receptors in the immune system.

G. Milligan, E. Alvarez-Curto, Elisa Alvarez-Curto, 2016, Biochemical Pharmacology.

High-Throughput Identification and Characterization of Novel, Species-selective GPR35 Agonists

Graeme Milligan, G. Milligan, A. Mackenzie, 2013, The Journal of Pharmacology and Experimental Therapeutics.

Apparent down-regulation of muscarinic acetylcholine receptors of neuroblastoma cells by pilocarpine is due to occluded agonist.

G. Milligan, P. Strange, 1983, European journal of pharmacology.

Complex Pharmacology of Free Fatty Acid Receptors.

B. Hudson, G. Milligan, B. Shimpukade, 2017, Chemical reviews.

Syddansk Universitet Complex Pharmacology of Free Fatty Acid Receptors

B. Hudson, G. Milligan, B. Shimpukade, 2017 .

The dynamic role of palmitoylation in signal transduction.

G. Milligan, A. Magee, M. Parenti, 1995, Trends in biochemical sciences.

Mechanism and Function of Drosophila capa GPCR: A Desiccation Stress-Responsive Receptor with Functional Homology to Human NeuromedinU Receptor

B. Hudson, G. Milligan, S. Davies, 2012, PloS one.

High-content assays for ligand regulation of G-protein-coupled receptors.

Graeme Milligan, G. Milligan, 2003, Drug discovery today.

The gonadotrophin-releasing hormone receptor of alpha T3-1 pituitary cells regulates cellular levels of both of the phosphoinositidase C-linked G proteins, Gq alpha and G11 alpha, equally.

B. H. Shah, G. Milligan, B H Shah, 1994, Molecular pharmacology.

Derivation of Endothelial Cells From Human Embryonic Stem Cells by Directed Differentiation: Analysis of MicroRNA and Angiogenesis In Vitro and In Vivo

G. Milligan, M. Houslay, R. Strehl, 2010, Arteriosclerosis, thrombosis, and vascular biology.

Mechanisms of multifunctional signalling by G protein-linked receptors.

G. Milligan, G. Milligan, 1993, Trends in pharmacological sciences.

Glucocorticoids regulate the amount of G proteins in rat aorta.

G. Milligan, C. Jones, R. Haigh, 1990, Journal of molecular endocrinology.

Gi-mediated activation of the p21ras-mitogen-activated protein kinase pathway by alpha 2-adrenergic receptors expressed in fibroblasts.

G. Milligan, P. Hordijk, W. Moolenaar, 1993, The Journal of biological chemistry.

High-content screening of feeder-free human embryonic stem cells to identify pro-survival small molecules.

G. Milligan, M. Houslay, P. D. Andrews, 2010, The Biochemical journal.

Presynaptic Control of Striatal Glutamatergic Neurotransmission by Adenosine A1–A2A Receptor Heteromers

Graeme Milligan, Francisco Ciruela, Meritxell Canals, 2006, The Journal of Neuroscience.

Selective activation of a chimeric Gi1/Gs G protein alpha subunit by the human IP prostanoid receptor: analysis using agonist stimulation of high affinity GTPase activity and [35S]guanosine-5'-O-(3-thio)triphosphate binding.

S. Rees, G. Milligan, C. Fong, 1998, Molecular pharmacology.

The long isoform of the rat thyrotropin-releasing hormone receptor down-regulates Gq proteins.

G. Milligan, J. Zabavnik, K. Eidne, 1994, The Journal of biological chemistry.

G‐proteins and G‐protein subunits mediating cholinergic inhibition of N‐type calcium currents in sympathetic neurons

G. Milligan, J. Haley, P. Delmas, 1998, The European journal of neuroscience.

The Use of Spatial Intensity Distribution Analysis to Examine G Protein-Coupled Receptor Oligomerization

G. Milligan, R. Ward, J. Pediani, 2017 .

The chemokine receptor CCX‐CKR mediates effective scavenging of CCL19 in vitro

Graeme Milligan, G. Milligan, S. Milasta, 2006, European journal of immunology.

Kinetic analysis of the internalization and recycling of [3H]TRH and C-terminal truncations of the long isoform of the rat thyrotropin-releasing hormone receptor-1.

G. Milligan, T. Drmota, 2000, The Biochemical journal.

Biphasic regulation of adenylate cyclase by cholera toxin in neuroblastoma x glioma hybrid cells is due to the activation and subsequent loss of the alpha subunit of the stimulatory GTP binding protein (GS).

G. Milligan, K. MacLeod, 1990, Cellular signalling.

Requirements and ontology for a G protein-coupled receptor oligomerization knowledge base

Michel Bouvier, Susan R. George, Jonathan A. Javitch, 2007, BMC Bioinformatics.

Oligomerisation of G-protein-coupled receptors.

G. Milligan, G Milligan, 2001, Journal of cell science.

FFA4/GPR120: Pharmacology and Therapeutic Opportunities

B. Hudson, G. Milligan, A. Tobin, 2017, Trends in pharmacological sciences.

FFA 4 / GPR 120 : Pharmacology and Therapeutic Opportunities

B. Hudson, G. Milligan, A. Tobin, 2017 .

Dynamic protein acylation and the regulation of localization and function of signal-transducing proteins.

G. Milligan, S. Ley, A. Magee, 1995, Biochemical Society transactions.

G protein-coupled receptor 35: an emerging target in inflammatory and cardiovascular disease

G. Milligan, S. Nicklin, A. Mackenzie, 2015, Front. Pharmacol..

Cell surface delivery and structural re-organization by pharmacological chaperones of an oligomerization-defective α1b-adrenoceptor mutant demonstrates membrane targeting of GPCR oligomers

G. Milligan, M. Canals, J. López-Giménez, 2008, The Biochemical journal.

G protein-coupled receptor dimerisation: molecular basis and relevance to function.

Graeme Milligan, G. Milligan, 2007, Biochimica et biophysica acta.

G-proteins of rat liver membranes. Subcellular compartmentation and disposition in the plasma membrane

G. Milligan, W. H. Evans, N. Ali, 2004, Molecular and Cellular Biochemistry.

Chemogenetic analysis of how receptors for short chain fatty acids regulate the gut-brain axis

J. Riddell, B. Hudson, G. Milligan, 2020, bioRxiv.

Dominant portion of thyrotropin-releasing hormone receptor is excluded from lipid domains. Detergent-resistant and detergent-sensitive pools of TRH receptor and Gqalpha/G11alpha protein.

J. Novotný, G. Milligan, P. Svoboda, 2005, Journal of biochemistry.

Long-term agonist stimulation of IP prostanoid receptor depletes the cognate G(s)alpha protein in membrane domains but does not change the receptor level.

J. Novotný, G. Milligan, P. Svoboda, 2004, Biochimica et biophysica acta.

The human muscarinic M1 acetylcholine receptor, when expressed in CHO cells, activates and downregulates both Gqα and G11α equally and non‐selectively

G. Milligan, N. Buckley, I. Mullaney, 1993 .

Analysis of Protein Palmitoylation

G. Milligan, M. Grassie, 1996 .

Antibodies to the GTP binding protein, Go, antagonize noradrenaline-induced calcium current inhibition in NG108-15 hybrid cells

D. A. Brown, G. Milligan, I. McFadzean, 1989, Neuron.

S 14506: novel receptor coupling at 5-HT1A receptors

E. Jacoby, M. Millan, M. Spedding, 2001, Neuropharmacology.

The GTP-binding regulatory proteins of neuroblastoma x glioma, NG108-15, and glioma, C6, cells. Immunochemical evidence of a pertussis toxin substrate that is neither Ni nor No.

G. Milligan, P. Gierschik, A. Spiegel, 1986, FEBS letters.

Muscarinic M‐current inhibition via G alpha q/11 and alpha‐adrenoceptor inhibition of Ca2+ current via G alpha o in rat sympathetic neurones.

D. A. Brown, G. Milligan, Susan M. Jones, 1994, The Journal of physiology.

Signal sorting by G-protein-linked receptors.

G. Milligan, 1995, Advances in pharmacology.

[21] Specificity and functional applications of antipeptide antisera which identify G-protein α subunits

G. Milligan, 1994 .

Identification of Three Residues Essential for 5-Hydroxytryptamine 2A-Metabotropic Glutamate 2 (5-HT2A·mGlu2) Receptor Heteromerization and Its Psychoactive Behavioral Function*

S. Sealfon, R. Neve, Terrell Holloway, 2012, The Journal of Biological Chemistry.

Chimaeric Gα proteins: their potential use in drug discovery

S. Rees, G. Milligan, 1999 .

Gi3 does not contribute to the inhibition of adenylate cyclase when stimulation of an alpha 2-adrenergic receptor causes activation of both Gi2 and Gi3.

G. Milligan, S. McClue, E. Selzer, 1992, The Biochemical journal.

Gs alpha mediates epidermal growth factor-elicited stimulation of rat cardiac adenylate cyclase.

G. Milligan, T. Patel, B. G. Nair, 1990, The Journal of biological chemistry.

Identification of Two Distinct Isoforms of the Guanine Nucleotide Binding Protein Go in Neuroblastoma × Glioma Hybrid Cells: Independent Regulation During Cyclic AMP‐Induced Differentiation

G. Milligan, I. Mullaney, 1990, Journal of neurochemistry.

Distinct Agonist Regulation of Muscarinic Acetylcholine M2-M3 Heteromers and Their Corresponding Homomers*

G. Milligan, Sara Marsango, E. Alvarez-Curto, 2015, The Journal of Biological Chemistry.

Receptors as Kissing Cousins

G. Milligan, 2000, Science.

Angiotensin-(1-7) and angiotensin-(1-9): function in cardiac and vascular remodelling.

C. A. McKinney, G. Milligan, C. Loughrey, 2014, Clinical science.

Differential regulation of amounts of the guanine-nucleotide-binding proteins Gi and Go in neuroblastoma x glioma hybrid cells in response to dibutyryl cyclic AMP.

G. Milligan, A. Magee, C. Unson, 1988, The Biochemical journal.

Eukaryotic Translation Initiation Factor 3, Subunit a, Regulates the Extracellular Signal-Regulated Kinase Pathway

W. Kolch, G. Milligan, Tianrui Xu, 2011, Molecular and Cellular Biology.

When simple agonism is not enough: Emerging modalities of GPCR ligands

G. Milligan, N. J. Smith, K. Bennett, 2011, Molecular and Cellular Endocrinology.

Concurrent up-regulation of guanine-nucleotide-binding proteins Gi1α, Gi2α and Gi3α in adipocytes of hypothyroid rats

G. Milligan, E. Saggerson, 1990 .

Involvement of G‐protein αil subunits in activation of G‐protein gated inward rectifying K+ channels (GIRK1) by human NPY1 receptors

G. Milligan, G. Mcallister, G. Seabrook, 1995, British journal of pharmacology.

Alterations in the activity of adenylate cyclase and high affinity GTPase in Alzheimer's disease

J. Mcculloch, G. Milligan, B. Ross, 1993, Brain Research.

Chronic exposure of rat glioma C6 cells to cholera toxin induces loss of the alpha-subunit of the stimulatory guanine nucleotide-binding protein (Gs).

G. Milligan, J. Knowler, C. Loney, 1990, European journal of pharmacology.

Concerted stimulation and deactivation of pertussis toxin‐sensitive G proteins by chimeric G protein‐coupled receptor‐regulator of G protein signaling 4 fusion proteins: analysis of the contribution of palmitoylated cysteine residues to the GAP activity of RGS4

G. Milligan, K. Druey, R. Ward, 2003, Journal of neurochemistry.

The Use of Stably Transfected Cell Lines in the Analysis of Functional Interactions between Goα1 & Giα2 and the α2C10 Adrenoceptor

G. Milligan, M. Grassie, 1995 .

Immunoprecipitation of solubilized opioid receptor-Go protein complexes using specific GTP-binding protein antisera

G. Milligan, C. Zioudrou, Z. Georgoussi, 1994, Regulatory Peptides.

Expression of Two Isoforms of the Guanine Nucleotide Binding Protein,G o , IN NG108–15 Neuroglioma Cells

G. Milligan, I. Mullaney, 1991 .

Uncovering the Pharmacology of the G Protein-Coupled Receptor GPR40: High Apparent Constitutive Activity in Guanosine 5′-O-(3-[35S]thio)triphosphate Binding Studies Reflects Binding of an Endogenous Agonist

Leigh A. Stoddart, G. Milligan, Andrew J. Brown, 2007, Molecular Pharmacology.

Differential uncoupling of A1 adenosine and D2 dopamine receptors by suramin and didemethylated suramin (NF037).

G. Milligan, M. Freissmuth, C. Nanoff, 1998, Molecular pharmacology.

Differential Uncoupling of A 1 Adenosine and D 2 Dopamine Receptors by Suramin and Didemethylated Suramin ( NF 037 )

G. Milligan, M. Freissmuth, C. Nanoff, 1998 .

Biochemical approaches to examine the specificity of interactions between receptors and guanine nuclotide binding proteins.

B. H. Shah, G. Milligan, I. Mullaney, 1995, Journal of receptor and signal transduction research.

Guanine nucleotide regulatory protein alterations in young Milan hypertensive strain rats.

J. Connell, G. Milligan, C. J. Clark, 1994, Biochimica et biophysica acta.

Discovery of a Potent Thiazolidine Free Fatty Acid Receptor 2 Agonist with Favorable Pharmacokinetic Properties.

Christer S. Ejsing, G. Milligan, D. Bolognini, 2018, Journal of medicinal chemistry.

G-protein-coupled receptors for free fatty acids: nutritional and therapeutic targets

Graeme Milligan, Trond Ulven, B. Hudson, 2014, British Journal of Nutrition.

Defining the Molecular Basis for the First Potent and Selective Orthosteric Agonists of the FFA2 Free Fatty Acid Receptor*

Graeme Milligan, Trond Ulven, Irina G. Tikhonova, 2013, The Journal of Biological Chemistry.

Defining the molecular basis for the first potent and selective orthosteric agonists of the FFA 2 free fatty acid

B. Hudson, G. Milligan, R. Ward, 2013 .

The therapeutic potential of allosteric ligands for free fatty acid sensitive GPCRs.

B. Hudson, G. Milligan, T. Ulven, 2013, Current topics in medicinal chemistry.

Gi2a fused to the human mu opioid receptor differently

G. Milligan, B. Kieffer, D. Massotte, 2002 .

Allosteric modulation of heterodimeric G-protein-coupled receptors.

G. Milligan, N. J. Smith, 2007, Trends in pharmacological sciences.

Orexin-1 Receptor-Cannabinoid CB1 Receptor Heterodimerization Results in Both Ligand-dependent and -independent Coordinated Alterations of Receptor Localization and Function*

G. Milligan, J. Pediani, S. Milasta, 2006, Journal of Biological Chemistry.

μ-δ Opioid Receptor Functional Interaction: Insight Using Receptor-G Protein Fusions

G. Milligan, B. Kieffer, D. Massotte, 2006, Journal of Pharmacology and Experimental Therapeutics.

Coordinated agonist regulation of receptor and G protein palmitoylation and functional rescue of palmitoylation-deficient mutants of the G protein G11alpha following fusion to the alpha1b-adrenoreceptor: palmitoylation of G11alpha is not required for interaction with beta*gamma complex.

G. Milligan, J. Pediani, J. J. Carrillo, 2001, The Journal of biological chemistry.

Insights into ligand pharmacology using receptor-G-protein fusion proteins.

G. Milligan, G. Milligan, 2000, Trends in pharmacological sciences.

Agonist occupation of an alpha2A-adrenoreceptor-Gi1alpha fusion protein results in activation of both receptor-linked and endogenous Gi proteins. Comparisons of their contributions to GTPase activity and signal transduction and analysis of receptor-G protein activation stoichiometry.

S. Rees, G. Milligan, A. Wise, 1998, The Journal of biological chemistry.

Rescue of Functional Interactions between the α2A-Adrenoreceptor and Acylation-resistant Forms of Gi1α by Expressing the Proteins from Chimeric Open Reading Frames*

G. Milligan, A. Wise, 1997, The Journal of Biological Chemistry.

Immunoprecipitation of opioid receptor-Go-protein complexes using specific GTP-binding-protein antisera.

G. Milligan, G Milligan, C. Zioudrou, 1995, The Biochemical journal.

Opioid peptides promote cholera-toxin-catalysed ADP-ribosylation of the inhibitory guanine-nucleotide-binding protein (Gi) in membranes of neuroblastoma x glioma hybrid cells.

G. Milligan, F. McKenzie, 1988, Biochemical Journal.

DECONVOLUTION OF COMPLEX G PROTEIN-COUPLED RECEPTOR SIGNALING IN LIVE CELLS : TAKING ADVANTAGE OF THE RESOLVING POWER OF DYNAMIC MASS REDISTRIBUTION TECHNOLOGY

G. Milligan, E. Kostenis, K. Mohr, 2010 .

Agonist activation of the G protein‐coupled receptor GPR35 involves transmembrane domain III and is transduced via Gα13 and β‐arrestin‐2

G. Milligan, E. Alvarez-Curto, M. Canals, 2011, British journal of pharmacology.

Protein-protein interactions at G-protein-coupled receptors.

G. Milligan, Julia H. M. White, J. White, 2001, Trends in pharmacological sciences.

Chapter 10. Hetero-oligomerization of chemokine receptors.

G. Milligan, S. Appelbe, 2009, Methods in enzymology.

Agonists activate Gi1 alpha or Gi2 alpha fused to the human mu opioid receptor differently.

G. Milligan, B. Kieffer, D. Massotte, 2002, Journal of neurochemistry.

Inverse agonism at adrenergic and opioid receptors: studies with wild type and constitutively active mutant receptors.

G. Milligan, D. MacEwan, I. Mullaney, 1997, Receptors & channels.

Agonist activation of p42 and p44 mitogen-activated protein kinases following expression of the mouse delta opioid receptor in Rat-1 fibroblasts: effects of receptor expression levels and comparisons with G-protein activation.

G. Milligan, N. Anderson, I. Mullaney, 1996, The Biochemical journal.

An arginine residue is the site of receptor-stimulated, cholera toxin-catalysed ADP-ribosylation of pertussis toxin-sensitive G-proteins.

G. Milligan, F. Mitchell, 1993, Cellular signalling.

Homo- and hetero-oligomeric interactions between G-protein-coupled receptors in living cells monitored by two variants of bioluminescence resonance energy transfer (BRET): hetero-oligomers between receptor subtypes form more efficiently than between less closely related sequences.

Graeme Milligan, S. Rees, G. Milligan, 2002, The Biochemical journal.

The α Subunit of Gq Contributes to Muscarinic Inhibition of the M-Type Potassium Current in Sympathetic Neurons

G. Milligan, J. Haley, Y. Vallis, 1998, The Journal of Neuroscience.

Therapeutic Opportunities and Challenges in Targeting the Orphan G Protein-Coupled Receptor GPR35

G. Milligan, A. Tobin, Tezz Quon, 2020, ACS pharmacology & translational science.

G protein‐coupled receptors not currently in the spotlight: free fatty acid receptor 2 and GPR35

G. Milligan, 2017, British journal of pharmacology.

The Antiallergic Mast Cell Stabilizers Lodoxamide and Bufrolin as the First High and Equipotent Agonists of Human and Rat GPR35

B. Hudson, G. Milligan, S. Nicklin, 2014, Molecular Pharmacology.

Microsoft Word-23820973-file00

B. Hudson, G. Milligan, Jennifer, 2013 .

Opioid regulation of mu receptor internalisation: relevance to the development of tolerance and dependence.

G. Milligan, J. López-Giménez, 2010, CNS & neurological disorders drug targets.

Human D2 and D4 dopamine receptors couple through β γ G-protein subunits to inwardly rectifying K+ channels (GIRK1) in a Xenopus oocyte expression system: selective antagonism by L-741,626 and L-745,870 respectively

G. Milligan, G. Mcallister, G. Seabrook, 1998, Neuropharmacology.

Visualizing differences in ligand‐induced β‐arrestin–GFP interactions and trafficking between three recently characterized G protein‐coupled receptors

Shelagh Wilson, G. Milligan, N. Evans, 2001, Journal of neurochemistry.

Palmitoylation Regulates Regulators of G-protein Signaling (RGS) 16 Function

G. Milligan, K. Druey, E. Fischer, 2003, Journal of Biological Chemistry.

A Molecular Mechanism for Sequential Activation of a G Protein-Coupled Receptor.

B. Hudson, G. Milligan, E. Kostenis, 2016, Cell chemical biology.

A novel mechanism for stepwise activation of a G protein-coupled receptor by a small molecule

B. Hudson, G. Milligan, E. Kostenis, 2016 .

Evidence for Distinct Antagonist-Revealed Functional States of 5-Hydroxytryptamine2A Receptor Homodimers

M. Loza, J. Brea, M. Cadavid, 2009, Molecular Pharmacology.

Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells

G. Milligan, E. Kostenis, K. Mohr, 2011, Nature Protocols.

On-target and off-target effects of novel orthosteric and allosteric activators of GPR84

S. McMahon, G. Milligan, A. Tobin, 2019, Scientific Reports.

Three classes of ligands each bind to distinct sites on the orphan G protein-coupled receptor GPR84

B. Hudson, G. Milligan, I. Tikhonova, 2017, Scientific Reports.

Context-Dependent Signaling of CXC Chemokine Receptor 4 and Atypical Chemokine Receptor 3

G. Milligan, M. Lohse, C. Hoffmann, 2019, Molecular Pharmacology.

Functional interactions between the α1b-adrenoceptor and Gα11 are compromised by de-palmitoylation of the G protein but not of the receptor

J. Novotný, G. Milligan, R. Ward, 2006 .

The Prevalence, Maintenance, and Relevance of G Protein–Coupled Receptor Oligomerization

G. Milligan, 2013, Molecular Pharmacology.

Signal transduction : a practical approach

G. Milligan, 1992 .

Reduction of adenylyl cyclase activity by cholera toxin in myeloid cells

M. Lanotte, G. Milligan, S. Hermouet, 1990, FEBS letters.

Identification of a novel site within G protein alpha subunits important for specificity of receptor-G protein interaction.

T. Frimurer, M. Rosenkilde, G. Milligan, 2004, Molecular pharmacology.

Angiotensin-( 1-7 ) and angiotensin-( 1-9 ) : function in cardiac and vascular remodeling

C. A. McKinney, G. Milligan, C. Loughrey, 2014 .

Up‐regulation of a constitutively active form of the β 2‐adrenoceptor by sustained treatment with inverse agonists but not antagonists

G. Milligan, D. MacEwan, 1996, FEBS letters.

The role of dimerisation in the cellular trafficking of G-protein-coupled receptors.

Graeme Milligan, G. Milligan, 2010, Current opinion in pharmacology.

Concurrent down-regulation of IP prostanoid receptors and the alpha-subunit of the stimulatory guanine-nucleotide-binding protein (Gs) during prolonged exposure of neuroblastoma x glioma cells to prostanoid agonists. Quantification and functional implications.

G. Milligan, F. McKenzie, I. Mullaney, 1992, The Biochemical journal.

Receptor availability defines the extent of agonist‐mediated G‐protein down‐regulation in neuroblastoma × glioma hybrid cells transfected to express the β2‐adrenoceptor

G. Milligan, Gun-Do Kim, Gun-Do Kim, 1994, FEBS letters.

Equivalent regulation of wild type and an epitope‐tagged variant of Gsα by the IP prostanoid receptor following expression in neuroblastoma × glioma hybrid, NG108‐15, cells

G. Milligan, I. Mullaney, 1994, FEBS Letters.

β-Arrestin 1 and Gαq/11 Coordinately Activate RhoA and Stress Fiber Formation following Receptor Stimulation*

J. Violin, R. Lefkowitz, G. Milligan, 2005, Journal of Biological Chemistry.

Molecular manipulation of G-protein-coupled receptors: a new avenue into drug discovery.

G. Milligan, G Milligan, M Sautel, 2000, Current medicinal chemistry.

Regulation of the stoichiometry of protein components of the stimulatory adenylyl cyclase cascade.

G. Milligan, D. MacEwan, I. Mullaney, 1998, Advances in pharmacology.

Agonist regulation of cellular Gs alpha-subunit levels in neuroblastoma x glioma hybrid NG108-15 cells transfected to express different levels of the human beta 2 adrenoceptor.

G. Milligan, E. Adie, 1994, The Biochemical journal.

Regulation of basal adenylate cyclase activity in neuroblastoma x glioma hybrid, NG108-15, cells transfected to express the human beta 2 adrenoceptor: evidence for empty receptor stimulation of the adenylate cyclase cascade.

G. Milligan, E. Adie, E. J. Adie, 1994, The Biochemical journal.

Prostanoid-mediated downregulation of Gs in NG108-15 cells.

G. Milligan, F. McKenzie, E. Adie, 1991, Biochemical Society transactions.

Foetal-calf serum stimulates a pertussis-toxin-sensitive high-affinity GTPase activity in rat glioma C6 BU1 cells.

G. Milligan, 1987, Biochemical Journal.

Non-Acidic Free Fatty Acid Receptor 4 Agonists with Antidiabetic Activity.

B. Hudson, G. Milligan, B. Shimpukade, 2016, Journal of medicinal chemistry.

The muscarinic M3 acetylcholine receptor exists as two differently sized complexes at the plasma membrane.

V. Raicu, G. Milligan, Tianrui Xu, 2013, The Biochemical journal.

Discovery of TUG-770: A Highly Potent Free Fatty Acid Receptor 1 (FFA1/GPR40) Agonist for Treatment of Type 2 Diabetes

B. Hudson, G. Milligan, E. Kostenis, 2013, ACS medicinal chemistry letters.

Applications of fluorescence and bioluminescence resonance energy transfer to drug discovery at G protein coupled receptors

G. Milligan, J. Pediani, E. Alvarez-Curto, 2010, Analytical and bioanalytical chemistry.

Differential effects of suramin on the coupling of receptors to individual species of pertussis-toxin-sensitive guanine-nucleotide-binding proteins.

G. Milligan, F. McKenzie, M. Wakelam, 1988, The Biochemical journal.

Interaction of the G‐protein G11α with receptors and phosphoinositidase C:

G. Milligan, M. Parenti, A. Wise, 1997 .

High-affinity interactions between human alpha1A-adrenoceptor C-terminal splice variants produce homo- and heterodimers but do not generate the alpha1L-adrenoceptor.

G. Milligan, J. Pediani, M. Fidock, 2004, Molecular pharmacology.

Syddansk Universitet Three classes of ligands each bind to distinct sites on the orphan G protein-coupled receptor GPR 84

B. Hudson, G. Milligan, I. Tikhonova, 2017 .

Dimerization of alpha1-adrenoceptors.

G. Milligan, J. Pediani, M. Fidock, 2004, Biochemical Society transactions.

Extracellular Ionic Locks Determine Variation in Constitutive Activity and Ligand Potency between Species Orthologs of the Free Fatty Acid Receptors FFA2 and FFA3*

B. Hudson, G. Milligan, I. Tikhonova, 2012, The Journal of Biological Chemistry.

Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and gqalpha /G11alpha induced by agonist stimulation.

J. Novotný, G. Milligan, G. Gould, 1999, The Biochemical journal.

Thyrotropin-releasing hormone-induced subcellular redistribution and down-regulation of G11alpha: analysis of agonist regulation of coexpressed G11alpha species variants.

G. Milligan, P. Svoboda, K. Eidne, 1996, Molecular pharmacology.

Widespread distribution of Gq alpha/G11 alpha detected immunologically by an antipeptide antiserum directed against the predicted C-terminal decapeptide.

G. Milligan, S. Arkinstall, M. Wakelam, 1991, FEBS letters.

Distribution of G-proteins in rat liver plasma-membrane domains and endocytic pathways.

G. Milligan, W. H. Evans, N. Ali, 1989, The Biochemical journal.

Defining the functional equivalence of wild-type and chemically engineered G protein-coupled receptors

G. Milligan, E. Alvarez-Curto, Elisa Alvarez-Curto, 2015 .

Immunological analysis of glucose transporters expressed in different regions of the rat brain and central nervous system.

G. Milligan, T. Jess, G. Gould, 1993, Biochemical and biophysical research communications.

G‐protein‐coupled receptors: an update

B B Fredholm, T. Hökfelt, B. Fredholm, 2007, Acta Physiologica.

Characterization of G proteins in rat myometrium A differential modulation of Gi2α and Gi3α during gestation

G. Milligan, S. Harbon, O. Goureau, 1991 .

The dually acylated NH2-terminal domain of gi1alpha is sufficient to target a green fluorescent protein reporter to caveolin-enriched plasma membrane domains. Palmitoylation of caveolin-1 is required for the recognition of dually acylated g-protein alpha subunits in vivo.

G. Milligan, M. Lisanti, F. Galbiati, 1999, The Journal of biological chemistry.

Free Fatty Acid Receptors

G. Milligan, I. Kimura, 2017 .

Comparative Analysis of the Efficacy of A1 Adenosine Receptor Activation of Gi/oα G Proteins following Coexpression of Receptor and G Protein and Expression of A1 Adenosine Receptor−Gi/oα Fusion Proteins†

S. Rees, G. Milligan, Melanie G. Lee, 1999 .

CXCR2 chemokine receptor antagonism enhances DOP opioid receptor function via allosteric regulation of the CXCR2–DOP receptor heterodimer

G. Milligan, S. Appelbe, G. Parenty, 2008, The Biochemical journal.

Kinetics of Ternary Complex Formation with Fusion Proteins Composed of the A1-Adenosine Receptor and G Protein α-Subunits*

G. Milligan, M. Freissmuth, C. Nanoff, 1999, The Journal of Biological Chemistry.

GTP analogues promote release of the alpha subunit of the guanine nucleotide binding protein, Gi2, from membranes of rat glioma C6 BU1 cells.

G. Milligan, L. Marshall, A. Spiegel, 1988, The Biochemical journal.

Receptor selectivity between the G proteins Gα12 and Gα13 is defined by a single leucine-to-isoleucine variation

David E. Gloriam, B. Hudson, G. Milligan, 2019, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

A single extracellular amino acid in Free Fatty Acid Receptor 2 defines antagonist species selectivity and G protein selection bias

B. Hudson, G. Milligan, D. Bolognini, 2017, Scientific Reports.

Ligand Regulation of the Quaternary Organization of Cell Surface M3 Muscarinic Acetylcholine Receptors Analyzed by Fluorescence Resonance Energy Transfer (FRET) Imaging and Homogeneous Time-resolved FRET*

G. Milligan, R. Ward, J. Pediani, 2010, The Journal of Biological Chemistry.

A general method to quantify ligand-driven oligomerization from fluorescence-based images

V. Raicu, G. Milligan, R. Ward, 2019, Nature Methods.

Evidence for the Existence of a CXCL17 Receptor Distinct from GPR35

G. Milligan, A. Mackenzie, J. Pease, 2018, The Journal of Immunology.

Muscarinic receptor oligomerization

G. Milligan, R. Ward, Sara Marsango, 2017, Neuropharmacology.

Effective Information Transfer from the α1b-Adrenoceptor to Gα11 Requires Both β/γ Interactions and an Aromatic Group Four Amino Acids from the C Terminus of the G Protein*

G. Milligan, J. Pediani, J. J. Carrillo, 2002, The Journal of Biological Chemistry.

Identification and Quantitation of G Protein α-Subunits

G. Milligan, I. Mullaney, 1997 .

The Use of Specific Antisera to Locate Functional Domains of Guanine Nucleotide Binding Proteins

G. Milligan, F. McKenzie, 1989 .

A Molecular Basis for Selective Antagonist Destabilization of Dopamine D3 Receptor Quaternary Organization

M. Millan, G. Milligan, R. Ward, 2017, Scientific Reports.

Using the Flp-In™ T-Rex™ system to regulate GPCR expression.

G. Milligan, R. Ward, E. Alvarez-Curto, 2011, Methods in molecular biology.

Phosphorylation of Gi in intact cells.

G. Milligan, M. Houslay, M. Bushfield, 1990, TIBS -Trends in Biochemical Sciences. Regular ed.

Beta 3-adrenoceptor agonist-induced down-regulation of Gs alpha and functional desensitization in a Chinese hamster ovary cell line expressing a beta 3-adrenoceptor refractory to down-regulation.

J. Chambers, S. Wilson, G. Milligan, 1994, The Biochemical journal.

G-proteins in experimental hypertension: a study of spontaneously hypertensive rat myocardial and renal cortical plasma membranes

J. Connell, G. Milligan, M. Houslay, 1993, Journal of Hypertension.

Ligands at the Free Fatty Acid Receptors 2/3 (GPR43/GPR41).

G. Milligan, D. Bolognini, E. Sergeev, 2017, Handbook of experimental pharmacology.

Developing Chemical Genetic Approaches to Explore G Protein-Coupled Receptor Function: Validation of the Use of a Receptor Activated Solely by Synthetic Ligand (RASSL)

G. Milligan, A. Tobin, M. Lohse, 2011, Molecular Pharmacology.

Immunohistochemical distribution of neurons containing the G-proteins Gq alpha/G11 alpha in the adult rat brain.

G. Milligan, J. Vanderhaeghen, C. Erneux, 1992, Neuroscience.

Intramolecular Fluorescence Resonance Energy Transfer (FRET) Sensors of the Orexin OX1 and OX2 Receptors Identify Slow Kinetics of Agonist Activation*

G. Milligan, Tianrui Xu, R. Ward, 2012, The Journal of Biological Chemistry.

Inverse agonist-induced up-regulation of the human beta2-adrenoceptor in transfected neuroblastoma X glioma hybrid cells.

G. Milligan, D. MacEwan, G Milligan, 1996, Molecular pharmacology.

Extracellular Loop 2 of the Free Fatty Acid Receptor 2 Mediates Allosterism of a Phenylacetamide Ago-Allosteric Modulator

Leigh A. Stoddart, B. Hudson, G. Milligan, 2011, Molecular Pharmacology.

The role of GPCR dimerisation/oligomerisation in receptor signalling.

G. Milligan, J. Pediani, M. Canals, 2006, Ernst Schering Foundation symposium proceedings.

Immunohistochemical distribution of neurons containing the G-proteins Gqα/G11α in the adult rat brain

G. Milligan, J. Vanderhaeghen, C. Erneux, 1992, Neuroscience.

G protein-coupled receptors : structure, signaling, and physiology

Graeme Milligan, G. Milligan, Sandra Siehler, 2010 .

Identification of Three Residues Essential for 5-HT 2 A-mGlu 2 Receptor Heteromerization and its Psychoactive Behavioral Function

S. Sealfon, R. Neve, G. Milligan, 2012 .

Mitogenic signalling by delta opioid receptors expressed in rat-1 fibroblasts involves activation of the p70s6k/p85s6k S6 kinase.

G. Milligan, N. Anderson, M. Wilson, 1997, The Biochemical journal.

Constitutive activity of GPR40/FFA1 intrinsic or assay dependent?

Leigh A. Stoddart, G. Milligan, 2010, Methods in enzymology.

Characterization of heterotrimeric G-proteins in adult Acanthocheilonema viteae.

G. Milligan, M. Harnett, W. Harnett, 1996, The Biochemical journal.

Use of [3H]Triphenylmethylphosphomum Cation for Estimating Membrane Potential in Neuroblastoma Cells

G. Milligan, P. Strange, Philip G. Strange, 1984, Journal of neurochemistry.

A pH-sensitive fluor, CypHer 5, used to monitor agonist-induced G protein-coupled receptor internalization in live cells.

G. Milligan, M. Cooper, M E Cooper, 2002, BioTechniques.

Growth Hormone Secretagogues and Growth Hormone Releasing Peptides Act As Orthosteric Super-Agonists but Not Allosteric Regulators for Activation of the G Protein Gαo1 by the Ghrelin Receptor

C. Langmead, G. Milligan, A. Wise, 2009, Molecular Pharmacology.

Novel Assay Technologies for the Discovery of G Protein-Coupled Receptor Drugs

G. Milligan, R. Ward, E. Alvarez-Curto, 2011 .

Novel approaches to enhance the detection of receptor constitutive activity and inverse agonists

G. Milligan, 2003 .

The specificity and molecular basis of α1-adrenoceptor and CXCR chemokine receptor dimerization

Graeme Milligan, G. Milligan, Shirley Wilson, 2007, Journal of Molecular Neuroscience.

Discovery of a Potent Free Fatty Acid 1 Receptor Agonist with Low Lipophilicity, Low Polar Surface Area, and Robust in Vivo Efficacy.

Christer S. Ejsing, B. Hudson, G. Milligan, 2016, Journal of Medicinal Chemistry.

Free fatty acid receptor 1 (FFA1/GPR40) agonists: mesylpropoxy appendage lowers lipophilicity and improves ADME properties.

B. Hudson, G. Milligan, E. Kostenis, 2012, Journal of medicinal chemistry.

Alpha 2-C10 adrenergic receptors expressed in rat 1 fibroblasts can regulate both adenylylcyclase and phospholipase D-mediated hydrolysis of phosphatidylcholine by interacting with pertussis toxin-sensitive guanine nucleotide-binding proteins.

G. Milligan, S. McClue, T. Jess, 1992, The Journal of biological chemistry.

Activation of the gonadotropin releasing hormone receptor of αT3 cells results in downregulation of the α subunits of both Gq/G11

B. H. Shah, G. Milligan, 1993 .

Novel mutation in the AVPR2 gene in a Danish male with nephrogenic diabetes insipidus caused by ER retention and subsequent lysosomal degradation of the mutant receptor

G. Milligan, D. Bichet, P. Deen, 2011, NDT plus.

the Medulla of Neurons in Superficial and Deeper Dorsal Horn of -Adrenoceptors Modulate NMDA-Evoked Responses

A. M. Peterson, E. Szabadi, M. Millan, 2015 .

The Highly Conserved DRY Motif of Class A G Protein-Coupled Receptors: Beyond the Ground State

Valérie Capra, Neil A. McDonald, Richard R Neubig, 2007, Molecular Pharmacology.

Effects of prolonged treatment of adipocytes with PGE1, N6-phenylisopropyl adenosine and nicotinic acid on G-proteins and antilipolytic sensitivity.

G. Milligan, A. Green, S. Belt, 1991, Biochemical Society transactions.

Functional interactions between the alpha1b-adrenoceptor and Galpha11 are compromised by de-palmitoylation of the G protein but not of the receptor.

J. Novotný, G. Milligan, R. Ward, 2006, Cellular signalling.

Cholera toxin treatment produces down-regulation of the alpha-subunit of the stimulatory guanine-nucleotide-binding protein (Gs).

G. Milligan, M. Wakelam, C. Unson, 1989, The Biochemical journal.

Treatment with triiodothyronine decreases the abundance of the α-subunits of Gi1 and Gi2 in the cerebral cortex

G. Milligan, E. Saggerson, M. Orford, 1992, Journal of the Neurological Sciences.

Expression of the Human β2‐Adrenoceptor in NCB20 Cells Results in Agonist Activation of Adenylyl Cyclase and Agonist‐Mediated Selective Down‐Regulation of Gsα

B. H. Shah, G. Milligan, A. Wise, 1995 .

Guanine nucleotide regulatory protein alterations in the Milan hypertensive rat strain

J. Connell, G. Milligan, C. J. Clark, 1993, Journal of Hypertension.

Non-equivalence of Key Positively Charged Residues of the Free Fatty Acid 2 Receptor in the Recognition and Function of Agonist Versus Antagonist Ligands*

B. Hudson, G. Milligan, A. Mackenzie, 2015, The Journal of Biological Chemistry.

Discovery and Characterization of Novel Antagonists of the Proinflammatory Orphan Receptor GPR84

Stuart P. McElroy, G. Milligan, A. Tobin, 2021, ACS pharmacology & translational science.

Inferring Signaling Pathway Topologies from Multiple Perturbation Measurements of Specific Biochemical Species

M. Girolami, W. Kolch, A. von Kriegsheim, 2010, Science Signaling.

Experimental challenges to targeting poorly characterized GPCRs: uncovering the therapeutic potential for free fatty acid receptors.

B. Hudson, G. Milligan, N. J. Smith, 2011, Advances in pharmacology.

Complex Pharmacology of Free Fatty Acid Receptors.

B. Hudson, G. Milligan, T. Ulven, 2017, Chemical reviews.

Metabolism meets immunity: The role of free fatty acid receptors in the immune system.

G. Milligan, Elisa Alvarez-Curto, 2016, Biochemical pharmacology.

Chemically engineering ligand selectivity at the free fatty acid receptor 2 based on pharmacological variation between species orthologs

B. Hudson, G. Milligan, E. Kostenis, 2012, The FASEB Journal.

, free fatty acid receptor 4 ( FFA 4 / GPR 120 ) The molecular basis of ligand interaction at Signal Transduction

B. Hudson, G. Milligan, T. Ulven, 2014 .

Capillary electrophoresis assay for G protein-coupled receptor-mediated GTPase activity.

R. Neubig, G. Milligan, R. Kennedy, 2007, Analytical chemistry.

The Pharmacology and Function of Receptors for Short-Chain Fatty Acids

G. Milligan, A. Tobin, D. Bolognini, 2016, Molecular Pharmacology.

Regulation of p42 and p44 MAP kinase isoforms in Rat-1 fibroblasts stably transfected with alpha 2C10 adrenoreceptors.

G. Milligan, N. Anderson, 1994, Biochemical and biophysical research communications.

Discovery of a Potent Thiazolidine Free Fatty Acid Receptor 2 Agonist with Favorable Pharmacokinetic Properties.

Christer S. Ejsing, G. Milligan, D. Bolognini, 2018, Journal of medicinal chemistry.

Localization of the succinate receptor in the distal nephron and its signaling in polarized MDCK cells.

G. Milligan, P. Deen, R. Fenton, 2009, Kidney international.

P273 Chemogenetic analysis of how receptors for short chain fatty acids regulate the gut-brain axis

B. Hudson, G. Milligan, A. Tobin, 2021, Posters.

The novel antidepressant, S35966, is a mixed serotonin and noradrenaline reuptake inhibitor and an antagonist at α2-adrenoceptors

D. Cussac, A. Gobert, J. Rivet, 2002, European Neuropsychopharmacology.

Real-time monitoring of redox changes in the mammalian endoplasmic reticulum

G. Milligan, J. Pediani, M. van Lith, 2011, Journal of Cell Science.

Regulation of cardiovascular remodeling by the counter-regulatory axis of the renin-angiotensin system.

C. A. McKinney, G. Milligan, S. Nicklin, 2013, Future cardiology.

Agonist regulation of adenylate cyclase activity in neuroblastoma x glioma hybrid NG108-15 cells transfected to co-express adenylate cyclase type II and the beta 2-adrenoceptor. Evidence that adenylate cyclase is the limiting component for receptor-mediated stimulation of adenylate cyclase activity.

G. Milligan, D. MacEwan, G. D. Kim, 1996, The Biochemical journal.

Defining the organizational structure of dopamine and muscarninic acetylcholine receptors

G. Milligan, Sara Marsango, G. Caltabiano, 2015, SpringerPlus.

Intramolecular Fluorescence Resonance Energy Transfer (FRET) Sensors of the Orexin OX1 and OX2 Receptors Identify Slow Kinetics of Agonist Activation*

G. Milligan, Tianrui Xu, R. Ward, 2012, The Journal of Biological Chemistry.

The Impact of G Proteins on Constitutive GPCR Activity

G. Milligan, 2006 .

The Molecular Basis of Oligomeric Organization of the Human M3 Muscarinic Acetylcholine Receptor

G. Milligan, R. Ward, Sara Marsango, 2015, Molecular Pharmacology.

Chapter 10. Hetero-oligomerization of chemokine receptors.

G. Milligan, S. Appelbe, 2009, Methods in enzymology.

Measurement of Agonist-Dependent and -Independent Signal Initiation of (cid:1) 1b -Adrenoceptor Mutants by Direct Analysis of Guanine Nucleotide Exchange on the G Protein G (cid:1) 11

G. Milligan, J. J. Carrillo, P. Stevens, 2002 .

Interactions between G-protein-coupled receptors and periplakin: a selective means to regulate G-protein activation.

G. Feng, G. Milligan, J. White, 2004, Biochemical Society transactions.

Presynaptic Control of Striatal Glutamatergic Neurotransmission by Adenosine A1–A2A Receptor Heteromers

F. Ciruela, R. Cunha, R. Luján, 2006, The Journal of Neuroscience.

Microsoft Word-73148042-file00.docx

G. Milligan, R. Ward, G. Caltabiano, 2015 .

Palmitoylation negative mutants of murine G11 alpha have decreased ability to interact with the plasma membrane when expressed in COS-1 cells.

G. Milligan, M. Parenti, A. Wise, 1995, Biochemical Society transactions.

Comparative analysis of the efficacy of A1 adenosine receptor activation of Gi/o alpha G proteins following coexpression of receptor and G protein and expression of A1 adenosine receptor-Gi/o alpha fusion proteins.

S. Rees, G. Milligan, A. Wise, 1999, Biochemistry.

Reduction in accumulation of [3H]triphenylmethylphosphonium cation in neuroblastoma cells caused by optical probes of membrane potential. Evidence for interactions between carbocyanine dyes and lipophilic anions.

G. Milligan, P. Strange, 1983, Biochimica et biophysica acta.

THEMED ISSUE: GPCR REVIEW G protein-coupled receptor hetero-dimerization: contribution to pharmacology and function

G. Milligan, 2009 .

A single amino acid determines preference between phospholipids and reveals length restriction for activation ofthe S1P4 receptor

James I. Fells, G. Milligan, A. Parrill, 2004, BMC Biochemistry.

Stochastic multidimensional hypercubes and inverse agonism.

A. IJzerman, G. Milligan, 2000, Trends in pharmacological sciences.