J. Ghuysen

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A quasi-Newton algorithm for first-order saddle-point location

P. Culot, G. Dive, V. H. Nguyen, 1992 .

Serine beta-lactamases and penicillin-binding proteins.

J. Ghuysen, Jean-Marie Ghuysen, 1991, Annual review of microbiology.

Serine beta-lactamases and penicillin-binding proteins.

J M Ghuysen, J. Ghuysen, 1991, Annual review of microbiology.

Molecular structures of penicillin-binding proteins and β-lactamases

J. Ghuysen, Jean-Marie Ghuysen, 1994 .

Molecular structures of penicillin-binding proteins and beta-lactamases.

J M Ghuysen, J. Ghuysen, 1994, Trends in microbiology.

Modular design of the Enterococcus hirae muramidase-2 and Streptococcus faecalis autolysin.

J. Ghuysen, B. Joris, S. Englebert, 1992, FEMS microbiology letters.

Modular design of theEnterococcus hiraemuramidase-2 andStreptococcus faecalisautolysin

J. Ghuysen, B. Joris, S. Englebert, 1992 .

Mechanism of acyl transfer by the class A serine beta-lactamase of Streptomyces albus G.

J. Frère, G. Dive, J. Ghuysen, 1991, The Biochemical journal.

Nucleotide sequence of the gene encoding the Streptomyces albus G β‐lactamase precursor

J. Frère, J. Ghuysen, B. Joris, 1987 .

A standard numbering scheme for the class A beta-lactamases.

J M Ghuysen, J. Frère, R. Levesque, 1991, The Biochemical journal.

Comparison of the sequences of class A beta-lactamases and of the secondary structure elements of penicillin-recognizing proteins

J M Ghuysen, J. Frère, J. Ghuysen, 1991, Antimicrobial Agents and Chemotherapy.

Identification of BlaR, the signal transducer for beta-lactamase production in Bacillus licheniformis, as a penicillin-binding protein with strong homology to the OXA-2 beta-lactamase (class D) of Salmonella typhimurium

J. Ghuysen, B. Joris, I. Curran, 1990, Journal of bacteriology.

Nucleotide sequence of the gene encoding the active-site serine β-lactamase from Streptomyces cacaoi

J. Frère, J. Ghuysen, B. Joris, 1988 .

Cloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular beta-lactamase from Streptomyces cacaoi.

J. Frère, J. Ghuysen, J. Dusart, 1987, Journal of general microbiology.

Exocellular β-Lactamases of Streptomyces albus G and Strains R39 and K11

J. Ghuysen, J. Dusart, Kenneth A. Johnson, 1973, Antimicrobial Agents and Chemotherapy.

The exocellular beta-lactamase of Streptomyces albus G. Purification, properties and comparison with the exocellular DD-carboxypeptidase.

J. Frère, J. Ghuysen, C. Duez, 1981, The Biochemical journal.

Cloning and amplified expression in Streptomyces lividans of a gene encoding extracellular beta-lactamase from Streptomyces albus G.

J. Frère, J. Martial, J. Ghuysen, 1986, Gene.

The active sites of the beta-lactamases of Streptomyces cacaoi and Streptomyces albus G.

J. Frère, J. Ghuysen, J. V. Van Beeumen, 1987, The Biochemical journal.

Primary structure of the Streptomyces R61 extracellular DD-peptidase. 2. Amino acid sequence data.

J. Frère, J. Ghuysen, J. V. Van Beeumen, 1987, European journal of biochemistry.

Beta-lactamase expression in Streptomyces cacaoi

J. Ghuysen, J. Dusart, M. Nakano, 1990, Journal of bacteriology.

The crystal structure of the beta-lactamase of Streptomyces albus G at 0.3 nm resolution.

J M Ghuysen, J. Frère, J. Ghuysen, 1987, The Biochemical journal.

Studies on the primary structures of the exocellular D-alanyl-D-alanine peptidases of Streptomyces strain R61 and Actinomadura strain R39.

J. Frère, J. Vandekerckhove, J. Ghuysen, 1981, Biochimica et biophysica acta.

Site-directed mutagenesis of dicarboxylic acid residues of the penicillin-binding module of the Escherichia coli penicillin-binding protein 3.

J. Ghuysen, M. Nguyen-Distèche, J. Ayala, 1993, FEMS microbiology letters.

Structure of the Cell Wall of Staphylococcus aureus StrainCopenhagen. VI. The Soluble Glycopeptide and Its Sequential Degradatuib by Peptidases

J. Strominger, J. Ghuysen, D. Tipper, 1965 .

Acetylhexosamine compounds enzymically released from Micrococcus lysodeikticus cell walls. III. The structure of DI- and tetra-saccharides released from cell walls by lysozyme and Streptomyces F1 enzyme.

J. Ghuysen, M. Salton, 1960, Biochimica et biophysica acta.

Acetylhexosamine compounds enzymically released from Micrococcus lysodeikticus cell walls. I. Isolation and composition of acetylhexosamine and acetylhexosamine-peptide complexes.

J. Ghuysen, M. Salton, 1960, Biochimica et biophysica acta.

The catalytic, glycosyl transferase and acyl transferase modules of the cell wall peptidoglycan‐polymerizing penicillin‐binding protein 1b of Escherichia coli

J. Ghuysen, J. V. Van Beeumen, M. Terrak, 1999, Molecular microbiology.

Penicillin‐Binding Proteins

J. Ghuysen, 2002 .

Nucleotide sequences of the pbpX genes encoding the penicillin‐binding proteins 2x from Streptococcus pneumoniae R6 and a cefotaxime‐resistant mutant, C506

J. Ghuysen, B. Joris, G. Laible, 1989, Molecular microbiology.

Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism

J. Ghuysen, 1968 .

Cloning and sequencing of the low-affinity penicillin-binding protein 3r-encoding gene of Enterococcus hirae S185: modular design and structural organization of the protein

G. Piras, J. Ghuysen, A. El Kharroubi, 1993, Journal of bacteriology.

The active centres in penicillin-sensitive enzymes.

J. Frère, J. Ghuysen, H. Perkins, 1980, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

Penicillins and Δ3-cephalosporins as inhibitors and mechanism-based inactivators of D-alanyl-D-Ala peptidases

J. Frère, J. Ghuysen, O. Dideberg, 1981 .

The penicillin-binding site in the exocellular DD-carboxypeptidase-transpeptidase of Actinomadura R39.

J. Frère, J. Ghuysen, J. V. Van Beeumen, 1981, The Biochemical journal.

Lack of Cell Wall Peptidoglycan versus Penicillin Sensitivity: New Insights into the Chlamydial Anomaly

J. Ghuysen, C. Goffin, 1999, Antimicrobial Agents and Chemotherapy.

Purification of DD-carboxypeptidases from Streptomyces strains R61 and K15 by antigen-antibody affinity chromatography

J. Ghuysen, A. Marquet, M. Leyh-Bouille, 1978 .

Mechanisms of Enzymatic Bacteriolysis

J. Strominger, J. Ghuysen, 1967 .

The bimodular G57-V577 polypeptide chain of the class B penicillin-binding protein 3 of Escherichia coli catalyzes peptide bond formation from thiolesters and does not catalyze glycan chain polymerization from the lipid II intermediate

J. Frère, J. Ghuysen, J. V. Van Beeumen, 1997, Journal of bacteriology.

Induction of β-lactamase and low-affinity penicillin binding protein 2' synthesis in gram-positive bacteria

J. Ghuysen, B. Joris, K. Hardt, 1994 .

Mechanisms of enzymatic bacteriaolysis. Cell walls of bacteri are solubilized by action of either specific carbohydrases or specific peptidases.

J. Strominger, J. Ghuysen, 1967, Science.

Fractionation of the DD-carboxypeptidase-transpeptidase activities solubilized from membranes of Escherichia coli K12, strain 44.

J. Ghuysen, K. Kim, J. Coyette, 1974, European journal of biochemistry.

Solubilization and isolation of the membrane-bound DD-carboxypeptidase of Streptococcus faecalis ATCC9790. Properties of the purified enzyme.

J. Ghuysen, J. Coyette, R. Fontana, 1978, European journal of biochemistry.

On the DD-carboxypeptidase enzyme system of Streptomyces strain K15.

J. Ghuysen, M. Nguyen-Distèche, M. Leyh-Bouille, 2005, European journal of biochemistry.

The exocellular DD‐carboxypeptidase of Streptomyces albus G: A metallo (Zn2+) enzyme

J. Frère, J. Ghuysen, B. Joris, 1980, FEBS letters.

The 4.5 Å resolution structure analysis of the exocellular DD‐carboxypeptidase of Streptomyces albus G

J. Frère, J. Ghuysen, P. Charlier, 1980, FEBS Letters.

Bacterial walls and membranes

J. Ghuysen, 1971 .

Enzymes that degrade bacterial cell walls

J. Strominger, J. Ghuysen, D. Tipper, 1966 .

Membrane-bound transpeptidase and penicillin binding sites in Streptomyces strain R61.

J. Ghuysen, J. Dusart, H. Perkins, 1974, European journal of biochemistry.

THE PENICILLIN RECEPTOR IN STREPTOMYCES *

J. Frère, J. Ghuysen, J. Dusart, 1974, Annals of the New York Academy of Sciences.

Structure of the wall peptidoglycan of Streptomyces R39 and the specificity profile of its exocellular DD-carboxypeptidase--transpeptidase for peptide acceptors.

J. Frère, J. Ghuysen, C. Duez, 1973, Biochemistry.

Properties and crystallization of a genetically engineered, water-soluble derivative of penicillin-binding protein 5 of Escherichia coli K12.

J. Ghuysen, U. Schwarz, P. Charlier, 1988, European journal of biochemistry.

Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism.

J. Ghuysen, 1968, Bacteriological reviews.

Structure of the Cell Wall of Staphylococcus aureus StrainCopenhagen. V. Isolation of Peptidases Active on the Peptide Moiety of the Cell Walls of Some Gram-Positive Bacteria

J. Strominger, J. Ghuysen, L. Dierickx, 1965 .

STRUCTURE OF THE CELL WALL OF STAPHYLOCOCCUS AUREUS, STRAIN COPENHAGEN. IV. THE TEICHOIC ACID-GLYCOPEPTIDE COMPLEX.

J. Strominger, J. Ghuysen, D. Tipper, 1965, Biochemistry.

STRUCTURE OF THE CELL WALL OF STAPHYLOCOCCUS AUREUS, STRAIN COPENHAGEN. I. PREPARATION OF FRAGMENTS BY ENZYMATIC HYDROLYSIS.

J. Strominger, J. Ghuysen, 1963, Biochemistry.

DISCUSSION PAPER: THE dd‐CARBOXYPEPTIDASE‐TRANSPEPTIDASE SYSTEM IN ESCHERICHIA COLI MUTANT STRAIN 44 *

J. Ghuysen, J. J. Pollock, M. Salton, 1974 .

Membrane-bound DD-carboxypeptidase and LD-transpeptidase of Streptococcus faecalis ATCC 9790.

J. Ghuysen, I. Polacheck, G. Shockman, 1974, European journal of biochemistry.

Sensitivity to ampicillin and cephalothin of enzymes involved in wall peptide crosslinking in Escherichia coli K12, strain 44.

J. Ghuysen, J. Coyette, J. J. Pollock, 1974, European journal of biochemistry.

Enzymes involved in wall peptide crosslinking in Escherichia coli K12, strain 44.

J. Ghuysen, J. Coyette, J. J. Pollock, 1974, European journal of biochemistry.

Peptide subunit. N(super x)-(L-alanyl-D-isoglutaminyl)L-lysyl-D-alanine in cell wall peptidoglycans of Staphylococcus aureus strain Copenhagen, Micrococcus roseus R27, and Streptococcus pyogenes group A, typ 14

H. Heymann, J. Ghuysen, J. Petit, 1966 .

Structural Variations in Bacterial Cell Wall Peptidoglycans Studied with Streptomyces F1 endo-N-Acetylmuramidase

J. Ghuysen, J. Petit, R. Tinelli, 1966 .

Structure of the cell wall of Micrococcus lysodeikticus. I. Study of the structure of the glycan.

J. Ghuysen, D. Tipper, M. Leyh-Bouille, 1966, Biochemistry.

Peptide cross-links in bacterial cell wall peptidoglycans studied with specific endopeptidases from Streptomyces albus G.

J. Ghuysen, E. Vegas Muñoz, J. Petit, 1966, Biochemistry.

Staphylolytic Enzyme from Chalaropsis: Mechanism of Action

J. Strominger, J. Ghuysen, D. Tipper, 1964, Science.

Structure of the cell walls of Micrococcus lysodeikticus. 3. Isolation of a new peptide dimer, N-alpha-[L-alanyl-gamma-(alpha-D-glutamylglycine)]-L-lysyl-D-alanyl-N-alpha-[L-alanyl-gamma-(alpha-D-glutamylglycine)]-L-lysyl-D-alanine.

J. Ghuysen, E. Bricas, M. Leyh-Bouille, 1968, Biochemistry.

The Cell Wall Peptidoglycan of Bacillus megaterium KM. I. Studies on the Stereochemistry of α,α'-Diaminopimelic Acid

J. Ghuysen, E. Bricas, P. Dézelée, 1967 .

Substituents on the alpha-carboxyl group of D-glutamic acid in the peptidoglycan of several bacterial cell walls.

J. Strominger, J. Ghuysen, D. Tipper, 1967, Biochemistry.

Structure of the cell wall of Staphylococcus aureus, strain Copenhagen. IX. Teichoic acid and phage adsorption.

J. Ghuysen, J. Coyette, J. Coyette, 1968, Biochemistry.

The peptide N alpha-(L-alanyl-D-isoglutaminyl)-N epsilon-(D-isoasparaginyl)-L-lysyl-D-alanine and the disaccharide N-acetylglucosaminyl-beta-1,4-N-acetylmuramic acid in cell wall peptidoglycan of Streptococcus faecalis strain ATCC 9790.

J. Ghuysen, G. Shockman, E. Bricas, 1967, Biochemistry.

Structure of the Cell Wall of Staphylococcus aureus, Strain Copenhagen. III. Further Studies of the Disaccharides*

J. Strominger, J. Ghuysen, D. Tipper, 1965 .

Exploration of active sites of DD-peptidases

J. Ghuysen, 1984 .

Characterization of the Micrococcus lysodeikticus type of peptidoglycan in walls of other Micrococcaceae.

J. Ghuysen, J. N. Campbell, M. Leyh-Bouille, 1969, Biochemistry.

ON THE LINKAGE BETWEEN TEICHOIC ACID AND THE GLYCOPEPTIDE IN THE CELL WALL OF STAPHYLOCOCCUS AUREUS.

J. Strominger, J. Ghuysen, J. L. Strominger, 1963, Biochemical and Biophysical Research Communications - BBRC.

Transpeptidase activity of Streptomyces D-alanyl-D carboxypeptidases.

J. Frère, J. Ghuysen, J. J. Pollock, 1972, Proceedings of the National Academy of Sciences of the United States of America.

The concept of the penicillin target from 1965 until today. The thirteenth marjory stephenson memorial lecture.

J. Ghuysen, 1977, Journal of general microbiology.

Stability of D-5,5-dimethyl-delta2-thiazoline-4-carboxylic acid in relation to its possible occurrence as a degradation product of penicillin by the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61 and the membrane-bound dd-carboxypeptidase from Bacillus stearothermophilus.

J. Frère, J. Ghuysen, B. Meesschaert, 1978, The Journal of biological chemistry.

Fragmentation of benzylpenicillin after interaction with the exocellular DD-carboxypeptidase-transpeptidases of Streptomyces R61 and R39

J. Frère, J. Ghuysen, H. Perkins, 1975, Nature.

ALKALINE DEGRADATION OF THE PHOSPHOMUCOPOLYSACCHARIDE FROM CELL WALLS OF BACILLUS MEGATERIUM KM.

J. Ghuysen, 1964, Biochimica et biophysica acta.

Penicillin-sensitive DD-carboxypeptidases from Streptomyces strains R39 and K11.

J. Frère, J. Ghuysen, H. Perkins, 1972, Biochemistry.

Crystallographic data for the DD-carboxypeptidase-endopeptidase of low penicillin sensitivity excreted by Streptomyces albus g.

J. Frère, J. Ghuysen, O. Dideberg, 1979, Journal of molecular biology.

Crystallographic data for a penicillin receptor : exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61.

J. Frère, J. Ghuysen, N. Murthy, 1979, Journal of molecular biology.

Wall peptidoglycan in Aerococcus viridans strains 201 Evans and ATCC 11563 and in Gaffkya homari strain ATCC 10400.

O. Kandler, J. Ghuysen, M. Nakel, 1971, Biochemistry.

Structure of the walls of Lactobacillus acidophilus strain 63 AM Gasser.

J. Ghuysen, J. Coyette, J. Coyette, 1970, Biochemistry.

LL-diaminopimelic acid containing peptidoglycans in walls of Streptomyces sp. and of Clostridium perfringens (type A).

J. Ghuysen, D. Tipper, R. Tinelli, 1970, Biochemistry.

Structure of the meso-diaminopimelic acid containing peptidoglycans in Escherichia coli B and Bacillus megaterium KM.

J. Ghuysen, L. Elbaz, J. Petit, 1969, Biochemistry.

The penicillin-binding proteins in Streptococcus faecalis ATCC 9790.

J. Ghuysen, J. Coyette, R. Fontana, 1980, European journal of biochemistry.

Use of model enzymes in the determination of the mode of action of penicillins and delta 3-cephalosporins.

J. Frère, J. Ghuysen, J. Dusart, 1979, Annual review of biochemistry.

Interaction between beta-lactam antibiotics and exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61.

J. Frère, J. Ghuysen, H. Perkins, 1974, European journal of biochemistry.

On the Streptomyces albus G DD carboxypeptidase mechanism of action of penicillin, vancomycin, and ristocetin.

K. Schleifer, O. Kandler, J. Ghuysen, 1970, Biochemistry.

Cell walls of Streptococcus pyogenes, type 14. C polysaccharide-peptidoglycan and G polysaccharide-peptidoglycan complexes.

H. Heymann, J. Ghuysen, E. Vegas Muñoz, 1967, Biochemistry.

The structure of di- and tetra-saccharides released from cell walls by lysozyme and streptomyces F1 enzyme and the β (1→4) N-acetylhexosaminidase activity of these enzymes

J. Ghuysen, M. Salton, 1959 .

The non-penicillin-binding module of the tripartite penicillin-binding protein 3 of Escherichia coli is required for folding and/or stability of the penicillin-binding module and the membrane-anchoring module confers cell septation activity on the folded structure

J. Ghuysen, M. Terrak, C. Fraipont, 1996, Journal of bacteriology.

The D-Alanyl-D-Alanine-Cleaving Peptidases and β-Lactamases. Structure and Mechanism

J. Ghuysen, 1987 .

B-Lactam Antibiotics as Carbonyl Donors of the Active-Site Serine B-Lacta-Mases, DD-Peptidases and LL-Peptidases

J. Ghuysen, 1987 .

Overexpression, solubilization and refolding of a genetically engineered derivative of the penicillin‐binding protein 3 of Escherichia coli K12

M. Inouye, J. Ghuysen, H. Hara, 1988, Molecular microbiology.

Interactions between beta-lactam antibiotics and isolated membranes of Streptococcus faecalis ATCC 9790.

J. Ghuysen, J. Coyette, B. Meesschaert, 1977, European journal of biochemistry.

Characterization of an Enterococcus hirae penicillin-binding protein 3 with low penicillin affinity

G. Piras, J. Ghuysen, J. V. Van Beeumen, 1990, Journal of bacteriology.

Modular Design of the Bi(Multi?)Functional Penicillin-Binding Proteins

G. Piras, J. Ghuysen, B. Joris, 1993 .

Dual multimodular class A penicillin-binding proteins in Mycobacterium leprae

S. Cole, J. Ghuysen, B. Joris, 1997, Journal of bacteriology.

Overexpression , solubiiization and refolding of a genetically engineered derivative of the penicillin-binding protein 3 of Escherichia coli K 12

M. Inouye, J. Ghuysen, H. Hara, 2010 .

Acetylhexosamine compounds enzymically released from Micrococcus lysodeikticus cell walls. II. Enzymic sensitivity of purified acetylhexosamine and acetylhexosamine-peptide complexes.

J. Ghuysen, 1960, Biochimica et biophysica acta.

An improved technique for the preparation of Streptomyces peptidases and N-acetylmuramyl-l-alanine amidase active on bacterial wall peptidoglycans.

J. Ghuysen, J. Coyette, L. Dierickx, 1969, Biochemistry.

Primary structure of the wall peptidoglycan of leprosy-derived corynebacteria

J. Ghuysen, C. Cocito, M. Leyh-Bouille, 1981, Journal of bacteriology.

The exchange reaction of peptides R-D-alanyl-D-alanine with D-[14C]alanine to R-D-alanyl-D-[14C]alanine and D-alanine, catalysed by the membranes of Streptococcus faecalis ATCC 9790.

J. Ghuysen, J. Coyette, H. Perkins, 1977, European journal of biochemistry.

Occurrence of D-alanyl-(D)-meso-diaminopimelic acid and meso-diaminopimelyl-meso-diaminopimelic acid interpeptide linkages in the peptidoglycan of Mycobacteria.

E. Lederer, J. Ghuysen, J. Petit, 1974, Biochemistry.

The wall peptidoglycans of Neisseria perflava, Moraxella glucidolytica, Pseudomonas alcaligenes and Proteus vulgaris strain P18.

J. Ghuysen, J. Martín, M. Mock, 1973, European journal of biochemistry.

dd-Carboxypeptidase-Transpeptidase and Killing Site of β-Lactam Antibiotics in Streptomyces Strains R39, R61, and K11

J. Frère, J. Ghuysen, J. Dusart, 1973, Antimicrobial Agents and Chemotherapy.

Exocellular β-Lactamases of Streptomyces albus G and Strains R39 and K11

J. Ghuysen, J. Dusart, Kenneth A. Johnson, 1973, Antimicrobial Agents and Chemotherapy.

Structural investigations on cell walls of Nocardia sp. The wall lipid and peptidoglycan moieties of Nocardia kirovani.

J. Ghuysen, G. Michel, M. Guinand, 1972, European journal of biochemistry.

The cell envelope in Proteus vulgaris P 18. Isolation and characterization of the peptidoglycan component.

J. Ghuysen, M. Mock, R. Minck, 1971, Biochimica et biophysica acta.

Penicillin-sensitive DD-carboxypeptidase from Streptomyces strain R 61.

J. Ghuysen, J. Coyette, H. Perkins, 1971, Biochemistry.

Occurrence of N-glycolylmuramic acid in bacterial cell walls. A preliminary survey.

E. Lederer, J. Ghuysen, I. Azuma, 1970, Biochimica et biophysica acta.

Chemical structure of the cell wall of Mycobacterium smegmatis. I. Isolation and partial characterization of the peptidoglycan.

E. Lederer, J. Ghuysen, J. Petit, 1969, Biochemical and biophysical research communications.

The peptidoglycan in walls of Butyribacterium rettgeri.

K. Schleifer, O. Kandler, J. Ghuysen, 1969, Biochemistry.

Fate of thiazolidine ring during fragmentation of penicillin by exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61

J. Frère, J. Ghuysen, H. Vanderhaeghe, 1976, Nature.

Bacterial transpeptidases and penicillin.

J. Frère, J. Ghuysen, J. Dusart, 1975 .

Biochemistry and Comparative Genomics of SxxK Superfamily Acyltransferases Offer a Clue to the Mycobacterial Paradox: Presence of Penicillin-Susceptible Target Proteins versus Lack of Efficiency of Penicillin as Therapeutic Agent

J. Ghuysen, C. Goffin, 2002, Microbiology and Molecular Biology Reviews.

Streptomyces K15 d-Ala-d-Ala Transpeptidase

J. Ghuysen, 2013 .

Importance of the E-46-D-160 polypeptide segment of the non-penicillin-binding module for the folding of the low-affinity, multimodular class B penicillin-binding protein 5 of Enterococus hirae

J. Ghuysen, J. Coyette, M. Mollerach, 1996, Journal of bacteriology.

The precursor of the Streptomyces R61 DD‐peptidase containing a C‐terminal extension is inactive

J. Knowles, J. Frère, E. Komives, 1994, FEBS letters.

Binding site‐shaped repeated sequences of bacterial wall peptidoglycan hydrolases

J. Ghuysen, B. Joris, J. Lamotte‐Brasseur, 1994, FEBS letters.

Detailed Study of a Molecule in a Molecule N-Acetyl-L-Tryptophanamide in a Active-Site Model of Alpha-Chymotrypsin

G. Dive, J. Ghuysen, D. Dehareng, 1994 .

Primary structure of the Streptomyces R61 extracellular DD-peptidase. 1. Cloning into Streptomyces lividans and nucleotide sequence of the gene.

J. Frère, J. Martial, J. Ghuysen, 1994, European journal of biochemistry.

Primary and predicted secondary structures of the Actinomadura R39 extracellular DD-peptidase, a penicillin-binding protein (PBP) related to the Escherichia coli PBP4.

J. Frère, J. Ghuysen, J. V. Van Beeumen, 1992, The Biochemical journal.

The Enterococcus hirae R40 penicillin-binding protein 5 and the methicillin-resistant Staphylococcus aureus penicillin-binding protein 2' are similar.

G. Piras, J. Ghuysen, J. V. Van Beeumen, 1991, The Biochemical journal.

Amino acid sequence of the penicillin-binding protein/DD-peptidase of Streptomyces K15. Predicted secondary structures of the low Mr penicillin-binding proteins of class A.

J. Ghuysen, J. V. Van Beeumen, S. Houba, 1991, The Biochemical journal.

Conformational analysis of β and γ-lactam antibiotics

G. Dive, J. Ghuysen, J. Lamotte, 1991 .

Streptomyces K15 DD-peptidase-catalysed reactions with suicide beta-lactam carbonyl donors.

J. Ghuysen, M. Nguyen-Distèche, C. Bourguignon, 1986, The Biochemical journal.

On the structural analogy between D-alanyl-D-alanine terminated peptides and β-lactam antibiotics

G. Dive, J. Ghuysen, J. Brasseur, 1984 .

Mode of interaction between beta-lactam antibiotics and the exocellular DD-carboxypeptidase--transpeptidase from Streptomyces R39.

J. Frère, J. Ghuysen, C. Duez, 1976, The Biochemical journal.

Site-directed mutagenesis of penicillin-binding protein 3 of Escherichia coli: role of Val-545.

J. Ghuysen, M. Nguyen-Distèche, J. Ayala, 1994, FEMS microbiology letters.

Bacterial active-site serine penicillin-interactive proteins and domains: mechanism, structure, and evolution.

J. Ghuysen, 1988, Reviews of infectious diseases.

NMR evidence for the structure of the complex between penicillin and the DD‐carboxypeptidase of streptomyces R61

J. Frère, J. Ghuysen, J. Feeney, 1979, FEBS letters.

Degradation of benzylpenicillin by the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R 61.

J. Frère, J. Ghuysen, H. Vanderhaeghe, 1975, Archives internationales de physiologie et de biochimie.

Penicillin-binding proteins and carboxypeptidase/transpeptidase activities in Proteus vulgaris P18 and its penicillin-induced stable L-forms

J. Ghuysen, M. Nguyen-Distèche, A. Rousset, 1982, Journal of bacteriology.

The direction of peptide trimer synthesis from the donor—acceptor substrateN α‐(acetyl)‐N ε‐(glycyl)—L‐lysyl—D‐alanyl—D‐alanine by the exocellular DD‐car☐ypeptidase‐transpeptidase ofStreptomyces R61

J. Frère, J. Ghuysen, 1976, FEBS letters.

Overproduction and properties of the mannuronate alginate lyase AlxMB.

J. Ghuysen, C. Duez, M. Malissard, 1995, FEMS microbiology letters.

STRUCTURE OF THE CELL WALL OF STAPHYLOCOCCUS AUREUS, STRAIN COPENHAGEN. II. SEPARATION AND STRUCTURE OF DISACCHARIDES.

J. Strominger, J. Ghuysen, J M GHUYSEN, 1963, Biochemistry.

Wall autolysin of Lactobacillus acidophilus strain 63 AM gasser.

J. Ghuysen, J. Coyette, 1970, Biochemistry.

Structure, function, and fate of the BlaR signal transducer involved in induction of beta-lactamase in Bacillus licheniformis

J. Ghuysen, B. Joris, T. Kobayashi, 1992, Journal of bacteriology.

The peptidoglycan crosslinking enzyme system in Streptomyces strains R61, K15 and rimosus.

J. Ghuysen, J. Dusart, P. Reynolds, 1977, European journal of biochemistry.

Energy hypersurface local properties of the O2HF−1 rearrangement

P. Culot, G. Dive, J. Ghuysen, 1992 .

Penicillin binding proteins and model transpeptidase activity of the plasma membranes of Streptomyces strains R61 and rimosus

J. Ghuysen, J. Dusart, P. Reynolds, 1981 .

Interaction between penicillin and the DD-carboxypeptidase of the unstable L-form of Proteus mirabilis strain 19.

H. H. Martin, J. Frère, J. Ghuysen, 1978, European journal of biochemistry.

The peptidoglycan crosslinking enzyme system in Streptomyces strains R61, K15 and rimosus. Kinetic coefficients involved in the interactions of the membrane-bound transpeptidase with peptide substrates and beta-lactam antibiotics.

J. Ghuysen, J. Dusart, M. Leyh-Bouille, 1977, European journal of biochemistry.

The catalytic activity and penicillin sensitivity in the liquid and frozen states of membrane-bound and detergent-solubilised transpeptidase of Streptomyces R61.

J. Ghuysen, J. Dusart, H. Perkins, 1975, European journal of biochemistry.

Fluorescence and circular dichroism studies on the Streptomyces R61 DD-carboxypeptidase-transpeptidase. Penicillin binding by the enzyme.

J. Frère, J. Ghuysen, H. Perkins, 1973, The Biochemical journal.

Modular design of the Enterococcus hirae muramidase-2 and Streptococcus faecalis autolysin.

J. Ghuysen, B. Joris, S. Englebert, 1992, FEMS microbiology letters.

Site-directed mutagenesis of the Streptomyces R61 DD-peptidase. Catalytic function of the conserved residues around the active site and a comparison with class-A and class-C beta-lactamases.

J. Frère, J. Ghuysen, B. Joris, 1992, European journal of biochemistry.

Comparison oftheSequences ofClassA 1-Lactamases andofthe Secondary Structure Elements ofPenicillin-Recog nizing Proteins

J. Frère, J. Ghuysen, B. Joris, 1991 .

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J. Frère, J. Ghuysen, H. Perkins, 1976 .

Exocellular DD-carboxypeptidases-transpeptidases from Streptomyces.

J. Frère, J. Ghuysen, H. Perkins, 1976, Methods in enzymology.

Transcriptional analysis of the dd-peptidase/penicillin-binding protein-encoding dac gene of Streptomyces R61: Use of the promoter and signal sequences in a secretion vector

J. Ghuysen, J. Dusart, M. V. Lenzini, 1990, Molecular and General Genetics MGG.

Enzymatic method for rapid and sensitive determination of β-lactam antibiotics

J. Frère, J. Ghuysen, D. Klein, 1980 .

Cloning, nucleotide sequence and amplified expression of the gene encoding the extracellular metallo (Zn) DD-peptidase of Streptomyces albus G.

J. Ghuysen, J. Dusart, S. Houba, 1990, FEMS microbiology letters.

Primary structure of the Streptomyces R61 extracellular DD-peptidase. 1. Cloning into Streptomyces lividans and nucleotide sequence of the gene.

J. Frère, J. Martial, J. Ghuysen, 1987, European journal of biochemistry.

The pH dependence of the active-site serine DD-peptidase of Streptomyces R61.

J. Frère, J. Ghuysen, M. Nguyen-Distèche, 1987, European journal of biochemistry.

Interaction between monobactams and model d‐alanyl‐d‐alanine‐cleaving peptidases

J. Frère, J. Ghuysen, J. A. Kelly, 1984 .

Purification and properties of the exocellular β-lactamase of a Actinomadura strain R39

J. Frère, J. Ghuysen, J. Beeumen, 1982 .

Enzymatic Method for Rapid and Sensitive Determnination of

J. Ghuysen, D. Klein, 1980 .

Large scale preparation of purified exocellular DD‐carboxypeptidase–transpeptidase of Streptomyces strain R61

J. Ghuysen, J. Dusart, P. Sicard, 1978 .

The peptidoglycan crosslinking enzyme system in Streptomyces R61, K15 and rimosus. Immunological studies.

J. Frère, J. Ghuysen, J. Dusart, 1977, European journal of biochemistry.

Interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61, substrate and beta-lactam antibiotics. A choice of models.

J M Ghuysen, J. Frère, J. Ghuysen, 1975, European journal of biochemistry.

A donor‐acceptor substrate of the exocellular DD‐carboxypeptidase‐transpeptidase from streptomyces R61

J. Frère, J. Ghuysen, A. Zeiger, 1975, FEBS letters.

Molecular weight, amino acid composition and physicochemical properties of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39.

J. Frère, J. Ghuysen, R. Moreno, 1974, The Biochemical journal.

Binding of beta-lactam antibiotics to the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R39.

J. Frère, J. Ghuysen, P. Reynolds, 1974, The Biochemical journal.

Effects of donor and acceptor peptides on concomitant hydrolysis and transfer reactions catalyzed by the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R39.

J. Frère, J. Ghuysen, P. Reynolds, 1974, Biochemistry.

Molecular weight and amino acid composition of the exocellular DD-carboxypeptidase-transpeptidase of Streptomyces R61.

J. Frère, J. Ghuysen, H. Perkins, 1973, The Biochemical journal.

Kinetics of concomitant transfer and hydrolysis reactions catalysed by the exocellular DD-carboxypeptidase-transpeptidase of streptomyces R61.

J. Frère, J. Ghuysen, H. Perkins, 1973, The Biochemical journal.

Streptomyces DD-carboxypeptidases as transpeptidases. The specificity for amino compounds acting as carboxyl acceptors.

J. Frère, J. Ghuysen, H. Perkins, 1973, The Biochemical journal.

Peptide inhibitors of Streptomyces DD-carboxypeptidases.

J. Frère, J. Ghuysen, H. Perkins, 1973, The Biochemical journal.

Kinetics of interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61 and beta-lactam antibiotics. A choice of models.

J. Frère, J. Ghuysen, M. Iwatsubo, 1975, European journal of biochemistry.

Action of actinomycetin on bacterial cell walls

J. Ghuysen, M. Salton, 1957 .

Bacterial cell wall

John F. Kennedy, Charles J. Knill, Regine Hakenbeck, 1996 .

Importance of the two tryptophan residues in the Streptomyces R61 exocellular DD-peptidase.

J. Frère, J. Ghuysen, J. V. Van Beeumen, 1992, The Biochemical journal.

The complete amino acid sequence of the Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of streptomyces albus G.

J. Frère, J. Ghuysen, J. V. Van Beeumen, 2005, European journal of biochemistry.

Δ2- and Δ3-cephalosporins, penicillinate and 6-unsubstituted penems. Intrinsic reactivity and interaction with β-lactamases and d-alanyl-d-alanine-cleaving serine peptidases

J. Frère, J. Ghuysen, Jean-Marie Frère, 1982 .

Occurrence of a serine residue in the penicillin‐binding site of the exocellular DD‐carboxy‐peptidase‐transpeptidase from Streptomyces R61

J. Frère, J. Ghuysen, C. Duez, 1976, FEBS letters.

Polarization correction to the electrostatic potential at the CNDO and theab initio level. Influence of the basis set expansion

G. Dive, J. Ghuysen, D. Dehareng, 1991 .

Influence of the counterpoise correction on the optimized relative degrees of freedom in the H-bonded complex water-formamide

G. Dive, J. Ghuysen, D. Dehareng, 1992 .

Energy analysis on small to medium sized H-bonded complexes

G. Dive, J. Ghuysen, D. Dehareng, 1993 .

Streptomyces albus G serine beta-lactamase. Probing of the catalytic mechanism via molecular modelling of mutant enzymes.

J M Ghuysen, F. Jacob-Dubuisson, J. Frère, 1992, The Biochemical journal.

2.8-A Structure of penicillin-sensitive D-alanyl carboxypeptidase-transpeptidase from Streptomyces R61 and complexes with beta-lactams.

J M Ghuysen, J. Frère, J. Ghuysen, 1986, The Journal of biological chemistry.

Multimodular Penicillin-Binding Proteins: An Enigmatic Family of Orthologs and Paralogs

J. Ghuysen, C. Goffin, 1998, Microbiology and Molecular Biology Reviews.

Differential functionalities of amphiphilic peptide segments of the cell‐septation penicillin‐binding protein 3 of Escherichia coli

R. Brasseur, J. Ghuysen, X. Gallet, 2000, Molecular microbiology.

Penicillin and beyond: evolution, protein fold, multimodular polypeptides, and multiprotein complexes.

J. Ghuysen, B. Joris, P. Charlier, 1996, Microbial drug resistance.

Penicillin-binding proteins. Wall peptidoglycan assembly and resistance to penicillin: facts, doubts and hopes.

J M Ghuysen, J. Ghuysen, 1997, International journal of antimicrobial agents.

Beta-lactamases (Actinomycetes species).

J. Frère, J. Ghuysen, C. Duez, 1975, Methods in Enzymology.

The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R61 DD-peptidase family.

J M Ghuysen, J. Frère, G. Dive, 1988, The Biochemical journal.

Streptomyces K15 DD-peptidase-catalysed reactions with ester and amide carbonyl donors.

J. Frère, J. Ghuysen, M. Nguyen-Distèche, 1986, The Biochemical journal.

The penicillin sensory transducer, BlaR, involved in the inducibility of β‐lactamase synthesis in Bacillus licheniformis is embedded in the plasma membrane via a four‐α‐helix bundle

J. Frère, R. Brasseur, A. Fink, 1997, Molecular microbiology.

Structure of a Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase at 2.5 Å resolution

J. Frère, G. Dive, J. Ghuysen, 1982, Nature.

Penicillin target enzyme and the antibiotic binding site.

J M Ghuysen, J. Frère, J. Ghuysen, 1982, Science.

The bacterial DD-carboxypeptidase-transpeptidase enzyme system: A new insight into the mode of action of penicillin

J. Ghuysen, 1977 .

FROM ACTINOMADURA R39

J. Ghuysen, H. Perkins, 1981 .

Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism

J. Ghuysen, 1968 .

Conformational analysis of peptide substrates and inhibitors of the Zn2+ G and serine R61 D-alanyl-D-alanine peptidases.

J. Frère, J. Ghuysen, J. Lamotte‐Brasseur, 1981, European journal of biochemistry.

Study of the Zn-containing DD-carboxypeptidase of Streptomyces albus G by small-angle X-ray scattering in solution.

J. Frère, G. Barnickel, J. Ghuysen, 1984, European journal of biochemistry.

The cell wall peptidoglycan of Bacillus megaterium KM. I. Studies on the stereochemistry of alpha, alpha'-diaminopimelic acid.

J. Ghuysen, E. Bricas, P. Dézelée, 1967, Biochemistry.

Characterization of the sporulation-related gamma-D-glutamyl-(L)meso-diaminopimelic-acid-hydrolysing peptidase I of Bacillus sphaericus NCTC 9602 as a member of the metallo(zinc) carboxypeptidase A family. Modular design of the protein.

J. Ghuysen, B. Joris, L. Denoroy, 1993, The Biochemical journal.

Bacterial wall peptidoglycan, DD-peptidases and beta-lactam antibiotics.

J. Frère, J. Ghuysen, B. Joris, 1984, Scandinavian Journal of Infectious Diseases. Supplementum.

Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate.

J. Ghuysen, B. Joris, C. Duez, 1993, FEMS microbiology letters.

Biochemistry of the penicilloyl serine transferases

G. Dive, J. Ghuysen, 1994 .

[Presence of glycopeptide in the cell wall of Bdellovibrio bacteriovorus].

J. Ghuysen, M. Shilo, R. Tinelli, 1970, Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles.

Biosynthesis and assembly of bacterial cell walls

J. Ghuysen, 1977 .

Biosynthesis of Peptidoglycan

J. Ghuysen, 1973 .

B-Lactam Antibiotics

J. Frère, J. Ghuysen, 2004 .

Cloning and nucleotide sequence of the gene encoding the gamma-D-glutamyl-L-diamino acid endopeptidase II of Bacillus sphaericus.

J. Ghuysen, B. Joris, C. Duez, 1992, FEMS microbiology letters.

Engineering and overexpression of periplasmic forms of the penicillin-binding protein 3 of Escherichia coli.

J. Ghuysen, J. V. Van Beeumen, H. Hara, 1994, The Biochemical journal.

Enzymatic method for rapid and sensitive determination of beta-lactam antibiotics

J. Frère, J. Ghuysen, D. Klein, 1980, Antimicrobial Agents and Chemotherapy.

Mode of membrane insertion and sequence of a 32-amino acid peptide stretch of the penicillin-binding protein 4 of Enterococcus hirae.

G. Piras, J. Ghuysen, J. V. Van Beeumen, 1991, FEMS microbiology letters.

The Gene Encoding the Low-Affinity Penicillin-Binding Protein 3r in Enterococcus hirae S185R Is Borne on a Plasmid Carrying Other Antibiotic Resistance Determinants

J. Ghuysen, M. Martínez-Bueno, D. Raze, 1998, Antimicrobial Agents and Chemotherapy.

The importance of the negative charge of β‐lactam compounds for the inactivation of the active‐site serine DD‐peptidase of Streptomyces R61

J. Frère, J. Ghuysen, L. Varetto, 1987, FEBS letters.

Expression in Escherichia coli of the carboxy terminal domain of the BLAR sensory-transducer protein of Bacillus licheniformis as a water-soluble Mr 26,000 penicillin-binding protein.

J. Ghuysen, B. Joris, P. Ledent, 1990, FEMS microbiology letters.

Electrostatic potential maps at the quantum chemistry level of the active sites of the serine peptidases, alpha-chymotrypsin and subtilisin.

G. Dive, J. Ghuysen, J. Lamotte‐Brasseur, 1990, Journal of theoretical biology.

Identification and overexpression in Escherichia coli of a Mycobacterium leprae gene, pon1, encoding a high-molecular-mass class A penicillin-binding protein, PBP1

S. Cole, J. Ghuysen, J. V. Van Beeumen, 1996, Journal of Bacteriology.

Streptomyces K15 active-site serine DD-transpeptidase: specificity profile for peptide, thiol ester and ester carbonyl donors and pathways of the transfer reactions.

J. Frère, J. Ghuysen, C. Damblon, 1995, The Biochemical journal.

Active-site-serine D-alanyl-D-alanine-cleaving-peptidase-catalysed acyl-transfer reactions. Procedures for studying the penicillin-binding proteins of bacterial plasma membranes.

J. Frère, J. Ghuysen, J. Coyette, 1986, The Biochemical journal.

Engineering a novel β-Iactamase by a single point mutation

J. Frère, J. Ghuysen, B. Joris, 1990 .

Active-site-directed inactivators of the Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of Streptomyces albus G.

J. Frère, G. Dive, J. Ghuysen, 1984, Biochemical Journal.

Zinc d-Ala-d-Ala Carboxypeptidase

J. Frère, J. Ghuysen, P. Charlier, 2013 .

Zinc d-Ala-d-Ala carboxypeptidase (Streptomyces)

J. Ghuysen, 2004 .

Site-directed mutagenesis of the Actinomadura R39 DD-peptidase.

J. Frère, J. Ghuysen, S. Lepage, 1997, The Biochemical journal.

Enterococus hirae. class B penicillin-binding protein 5 of for the folding of the low-affinity, multimodular segment of the non-penicillin-binding module Importance of the E-46-D-160 polypeptide

J. Ghuysen, J. Coyette, M. Mollerach, 2014 .

Importance of the His-298 residue in the catalytic mechanism of the Streptomyces R61 extracellular DD-peptidase.

J M Ghuysen, J. Frère, J. Ghuysen, 1992, The Biochemical journal.

The life‐cycle proteins RodA of Escherichia coli and SpoVE of Bacillus subtilis have very similar primary structures

G. Dive, J. Ghuysen, B. Joris, 1990, Molecular microbiology.

Crystallographic mapping of beta-lactams bound to a D-alanyl-D-alanine peptidase target enzyme.

J. Frère, J. Ghuysen, J. Knox, 1989, Journal of molecular biology.

On the origin of bacterial resistance to penicillin: comparison of a beta-lactamase and a penicillin target.

J. Frère, J. Ghuysen, B. Joris, 1986, Science.

The exocellular DD-carboxypeptidase-endopeptidase from Streptomyces albus G. Purification and chemical properties.

J. Frère, J. Ghuysen, C. Duez, 1978, The Biochemical journal.

The exocellular DD-carboxypeptidase-endopeptidase of Streptomyces albus G. Interaction with beta-lactam antibiotics.

J. Frère, J. Ghuysen, F. Geurts, 1978, The Biochemical journal.

Fragmentation of penicillin catalysed by the exocellular DD‐carboxypeptidase‐transpeptidase of Streptomyces strain R61 isotopic study of hydrogen fixation on carbon 6

J. Frère, J. Ghuysen, 1978, FEBS Letters.

Cloning, sequencing and overexpression in Escherichia coli of the alginatelyase-encoding aly gene of Pseudomonas alginovora: identification of three classes of alginate lyases.

B. Henrissat, J. Ghuysen, P. Potin, 1996, The Biochemical journal.

Isolation of the membrane-bound 26 000-Mr penicillin-binding protein of Streptomyces strain K15 in the form of a penicillin-sensitive D-alanyl-D-alanine-cleaving transpeptidase.

J. Ghuysen, M. Nguyen-Distèche, M. Leyh-Bouille, 1982, The Biochemical journal.

[Data on the structure of disaccharide-peptide complexes liberated from the wall of Micrococcus lysodeikticus by the action of beta(1-4)N-acetylhexosaminidases].

J. Ghuysen, 1961, Biochimica et biophysica acta.

Substrate requirements of the Streptomyces albus G DD carboxypeptidase.

K. Schleifer, O. Kandler, J. Ghuysen, 1970, Biochemistry.

Antibiotics and peptidoglycan metabolism

J. Ghuysen, 1980 .

Interactions between non-classical β-lactam compounds and the β-lactamases of Actinomadura R39 and Streptomyces albus G

J. Frère, J. Ghuysen, C. Duez, 1981 .

Substrate Requirements of Glycosidases for Lytic Activity on Bacterial Walls

J. Ghuysen, 1974 .

Occurrence of Glucomuramic Acid in Gram-Positive Bacteria

J. Ghuysen, R. Wheat, 1971, Journal of bacteriology.

Interaction between non-classical beta-lactam compounds and the Zn2+-containing G and serine R61 and R39 D-alanyl-D-alanine peptidases.

J. Frère, J. Ghuysen, J. A. Kelly, 1981, The Biochemical journal.

Structure of the Cell Walls of Micrococcus lysodeikticus. III. Isolation of a New Peptide Dimer, Nα-[L-Alanyl-γ-(α-D-glutamyl-glycine )]-L-lysyl-D-alanyl-Nα- [L-alanyl-γ-(α-D-glutamyl-glycine)]-L-lysyl-D-alanine

J. Ghuysen, E. Bricas, M. Leyh-Bouille, 1968 .

Active-site and membrane topology of the DD-peptidase/penicillin-binding protein no. 6 of Enterococcus hirae (Streptococcus faecium) A.T.C.C. 9790.

G. Piras, J. Ghuysen, J. V. Van Beeumen, 1989, The Biochemical journal.