J. Lorsch

发表

J. Hackman, J. Lorsch, 1987 .

eIF4A is stimulated by the pre-initiation complex and enhances recruitment of mRNAs regardless of structural complexity

Colin Echeverría Aitken, A. Hinnebusch, J. Lorsch, 2017, bioRxiv.

1 " " eIF 4 A is stimulated by the pre-initiation complex and enhances recruitment of mRNAs regardless of structural 1 " complexity 2 " 3 "

Colin Echeverría Aitken, A. Hinnebusch, J. Lorsch, 2017 .

A mechanistic overview of translation initiation in eukaryotes

Jon R Lorsch, Colin Echeverría Aitken, J. Lorsch, 2012, Nature Structural &Molecular Biology.

The mechanism of eukaryotic translation initiation: new insights and challenges.

Jon R Lorsch, Alan G Hinnebusch, A. Hinnebusch, 2012, Cold Spring Harbor perspectives in biology.

Specific Domains in Yeast Translation Initiation Factor eIF4G Strongly Bias RNA Unwinding Activity of the eIF4F Complex toward Duplexes with 5′-Overhangs*

A. Hinnebusch, J. Lorsch, E. Park, 2012, The Journal of Biological Chemistry.

Communication between eukaryotic translation initiation factors 1 and 1A on the yeast small ribosomal subunit.

J. Lorsch, David Maag, 2003, Journal of molecular biology.

The molecular mechanics of eukaryotic translation.

Jon R Lorsch, J. Lorsch, Lee D. Kapp, 2003, Annual review of biochemistry.

A conformational change in the eukaryotic translation preinitiation complex and release of eIF1 signal recognition of the start codon.

J. Lorsch, David Maag, Christie A Fekete, 2005, Molecular cell.

The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosome.

Mikkel A. Algire, V. Ramakrishnan, J. Lorsch, 2007, Molecular cell.

Dissociation of eIF1 from the 40S ribosomal subunit is a key step in start codon selection in vivo.

Mikkel A. Algire, A. Hinnebusch, J. Lorsch, 2007, Genes & development.

Coupled Release of Eukaryotic Translation Initiation Factors 5B and 1A from 80S Ribosomes following Subunit Joining

Jon R. Lorsch, J. Lorsch, T. E. Dever, 2007, Molecular and Cellular Biology.

Pi release from eIF2, not GTP hydrolysis, is the step controlled by start-site selection during eukaryotic translation initiation.

Mikkel A. Algire, J. Lorsch, David Maag, 2005, Molecular cell.

In vitro evolution of new ribozymes with polynucleotide kinase activity

Jon R. Lorsch, Jack W. Szostak, J. Szostak, 1994, Nature.

eIF1 controls multiple steps in start codon recognition during eukaryotic translation initiation.

A. Hinnebusch, J. Lorsch, Y. Cheung, 2009, Journal of molecular biology.

Organizing Graduate Life Sciences Education around Nodes and Connections

J. Lorsch, David G. Nichols, 2011, Cell.

The beta propellers of eIF3b and eIF3i relocate together to the ribosomal intersubunit interface during translation initiation

A. Hinnebusch, V. Ramakrishnan, J. Lorsch, 2018, bioRxiv.

Active yeast ribosome preparation using monolithic anion exchange chromatography

Sarah E. Walker, J. Lorsch, Paul Yourik, 2016, RNA biology.

Inhibition of protein synthesis by aminoglycoside-arginine conjugates.

J. Pelletier, J. Lorsch, P. Garneau, 2002, RNA.

Molecular View of 43 S Complex Formation and Start Site Selection in Eukaryotic Translation Initiation*

J. Lorsch, T. E. Dever, T. Dever, 2010, The Journal of Biological Chemistry.

Distinct interactions of eIF4A and eIF4E with RNA helicase Ded1 stimulate translation in vivo

Suna P. Gulay, A. Hinnebusch, J. Lorsch, 2020, eLife.

Where to begin? The mechanism of translation initiation codon selection in eukaryotes.

Mikkel A. Algire, J. Lorsch, 2006, Current opinion in chemical biology.

Coordinated Movements of Eukaryotic Translation Initiation Factors eIF1, eIF1A, and eIF5 Trigger Phosphate Release from eIF2 in Response to Start Codon Recognition by the Ribosomal Preinitiation Complex*

A. Hinnebusch, J. Lorsch, J. Nanda, 2013, The Journal of Biological Chemistry.

β-Hairpin Loop of Eukaryotic Initiation Factor 1 (eIF1) Mediates 40 S Ribosome Binding to Regulate Initiator tRNAMet Recruitment and Accuracy of AUG Selection in Vivo * ♦

R. E. Luna, A. Hinnebusch, G. Wagner, 2013, The Journal of Biological Chemistry.

Communication between eukaryotic translation initiation factors 5 and 1A within the ribosomal pre-initiation complex plays a role in start site selection.

Mikkel A. Algire, J. Lorsch, David Maag, 2006, Journal of molecular biology.

Intragenic Suppressor Mutations Restore GTPase and Translation Functions of a Eukaryotic Initiation Factor 5B Switch II Mutant

J. Lorsch, B. Shin, T. E. Dever, 2006, Molecular and Cellular Biology.

Protein derivitization-expressed protein ligation.

J. Lorsch, Sarah F. Mitchell, 2014, Methods in enzymology.

Reconstitution of yeast translation initiation.

J. Lorsch, S. Kolitz, M. Acker, 2007, Methods in enzymology.

Labeling of a protein with fluorophores using maleimide derivitization.

J. Lorsch, J. Nanda, 2014, Methods in enzymology.

Identification and characterization of functionally critical, conserved motifs in the internal repeats and N-terminal domain of yeast translation initiation factor 4B (yeIF4B).

Sarah E. Walker, A. Hinnebusch, J. Lorsch, 2014, The Journal of Biological Chemistry.

Identification and Characterization of Functionally Critical, Conserved Motifs in the Internal Repeats and N-terminal Domain of Yeast Translation Initiation Factor 4B (yeIF4B)*

Sarah E. Walker, A. Hinnebusch, J. Lorsch, 2013, The Journal of Biological Chemistry.

Eukaryotic translation initiation factor eIF5 promotes the accuracy of start codon recognition by regulating Pi release and conformational transitions of the preinitiation complex

A. Hinnebusch, J. Lorsch, Fan Zhang, 2015, Nucleic acids research.

Eukaryotic translation initiation factor eIF5 promotes the accuracy of start codon recognition by regulating Pi release and conformational transitions of the preinitiation complex

A. Hinnebusch, J. Lorsch, Fan Zhang, 2014, Nucleic acids research.

ATP and GTP hydrolysis assays (TLC).

J. Lorsch, V. Rajagopal, Jon R Lorsch, 2013, Methods in enzymology.

4 The Mechanism of Translation Initiation in Eukaryotes

J. Lorsch, C. Hellen, T. Pestova, 2007 .

eIF3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex

Colin Echeverría Aitken, A. Hinnebusch, J. Lorsch, 2016 .

Chance and necessity in the selection of nucleic acid catalysts.

J R Lorsch, J W Szostak, J. Szostak, 1996, Accounts of chemical research.

Kinetic dissection of fundamental processes of eukaryotic translation initiation in vitro

J R Lorsch, D. Herschlag, J. Lorsch, 1999, The EMBO journal.

In vitro selection of RNA aptamers specific for cyanocobalamin.

J. Szostak, J. Lorsch, 1994, Biochemistry.

The DEAD box protein eIF4A. 2. A cycle of nucleotide and RNA-dependent conformational changes.

D. Herschlag, J. Lorsch, Daniel Herschlag, 1998, Biochemistry.

RNA purification--precipitation methods.

Sarah E. Walker, J. Lorsch, 2013, Methods in enzymology.

RNA Chaperones Exist and DEAD Box Proteins Get a Life

J. Lorsch, 2002, Cell.

Reverse transcriptase reads through a 2'-5'linkage and a 2'-thiophosphate in a template.

J R Lorsch, J W Szostak, D. Bartel, 1995, Nucleic acids research.

Functional interplay between DEAD-box RNA helicases Ded1 and Dbp1 in preinitiation complex attachment and scanning on structured mRNAs in vivo.

A. Hinnebusch, T. Preiss, J. Lorsch, 2019, Nucleic acids research.

Ribosome recycling step in yeast cytoplasmic protein synthesis is catalyzed by eEF3 and ATP

J. Lorsch, A. Kaji, K. Nielsen, 2010, Proceedings of the National Academy of Sciences.

Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition

A. Hinnebusch, V. Ramakrishnan, J. Lorsch, 2018, bioRxiv.

Molecular Architecture of a Eukaryotic Translational Initiation Complex

V. Ramakrishnan, Sjors H. W. Scheres, Jon R. Lorsch, 2013, Science.

Structural integrity of {alpha}-helix H12 in translation initiation factor eIF5B is critical for 80S complex stability.

J. Lorsch, B. Shin, M. Acker, 2011, RNA.

Regulatory elements in eIF1A control the fidelity of start codon selection by modulating tRNA(i)(Met) binding to the ribosome.

A. Hinnebusch, J. Lorsch, J. Nanda, 2010, Genes & development.

Kinetic analysis of late steps of eukaryotic translation initiation.

J. Lorsch, B. Shin, M. Acker, 2009, Journal of molecular biology.

rRNA Suppressor of a Eukaryotic Translation Initiation Factor 5B/Initiation Factor 2 Mutant Reveals a Binding Site for Translational GTPases on the Small Ribosomal Subunit

J. Lorsch, B. Shin, M. Acker, 2008, Molecular and Cellular Biology.

Should I Stay or Should I Go? Eukaryotic Translation Initiation Factors 1 and 1A Control Start Codon Recognition*

J. Lorsch, Sarah F. Mitchell, 2008, Journal of Biological Chemistry.

N‐ and C‐terminal residues of eIF1A have opposing effects on the fidelity of start codon selection

Jon R Lorsch, Alan G Hinnebusch, Mikkel A. Algire, 2007, The EMBO journal.

Interaction between Eukaryotic Initiation Factors 1A and 5B Is Required for Efficient Ribosomal Subunit Joining*

J. Lorsch, B. Shin, M. Acker, 2006, Journal of Biological Chemistry.

Uncoupling of Initiation Factor eIF5B/IF2 GTPase and Translational Activities by Mutations that Lower Ribosome Affinity

S. Burley, J. Lorsch, B. Shin, 2002, Cell.

Mechanism of ribosomal subunit joining during eukaryotic translation initiation.

Jon R Lorsch, J. Lorsch, M. Acker, 2008, Biochemical Society transactions.

Conformational Differences between Open and Closed States of the Eukaryotic Translation Initiation Complex

Colin Echeverría Aitken, A. Hinnebusch, V. Ramakrishnan, 2015, Molecular cell.

Structural Changes Enable Start Codon Recognition by the Eukaryotic Translation Initiation Complex

A. Hinnebusch, V. Ramakrishnan, J. Lorsch, 2014, Cell.

eIF1 discriminates against suboptimal initiation sites to prevent excessive uORF translation genome-wide

A. Hinnebusch, J. Lorsch, Fujun Zhou, 2020, RNA.

eIF1A residues implicated in cancer stabilize translation preinitiation complexes and favor suboptimal initiation sites in yeast

A. Hinnebusch, J. Lorsch, Fan Zhang, 2017, eLife.

Rps3/uS3 promotes mRNA binding at the 40S ribosome entry channel and stabilizes preinitiation complexes at start codons

Colin Echeverría Aitken, A. Hinnebusch, J. Lorsch, 2017, Proceedings of the National Academy of Sciences.

Conserved residues in yeast initiator tRNA calibrate initiation accuracy by regulating preinitiation complex stability at the start codon

A. Hinnebusch, J. Lorsch, S. Kolitz, 2014, Genes & development.

Enhanced eIF1 binding to the 40S ribosome impedes conformational rearrangements of the preinitiation complex and elevates initiation accuracy

R. E. Luna, A. Hinnebusch, G. Wagner, 2014, RNA.

The C-terminal domain of eukaryotic initiation factor 5 promotes start codon recognition by its dynamic interplay with eIF1 and eIF2β.

Jon R Lorsch, Assen Marintchev, Gerhard Wagner, 2012, Cell reports.

The C-Terminal Domain of Eukaryotic Initiation Factor 5 Promotes Start Codon Recognition by Its Dynamic Interplay with eIF 1 and eIF 2 b

R. E. Luna, A. Hinnebusch, S. Hyberts, 2012 .

Initiation Factor eIF2γ Promotes eIF2–GTP–Met-tRNAiMet Ternary Complex Binding to the 40S Ribosome

Sarah E. Walker, J. Lorsch, B. Shin, 2011, Nature Structural &Molecular Biology.

Initiation Factor eIF 2 γ Promotes eIF 2 – GTP – Met-tRNAiMet Ternary Complex Binding to the 40 S Ribosome

Sarah E. Walker, J. Lorsch, B. Shin, 2011 .

GTP-dependent recognition of the methionine moiety on initiator tRNA by translation factor eIF2.

Jon R Lorsch, J. Lorsch, Lee D. Kapp, 2004, Journal of molecular biology.

Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae

A. Hinnebusch, J. Lorsch, Fujun Zhou, 2019, BMC Biology.

Kinetic and thermodynamic characterization of the reaction catalyzed by a polynucleotide kinase ribozyme.

J. Szostak, J. Lorsch, 1995, Biochemistry.

Eukaryotic translation initiation factor 3 plays distinct roles at the mRNA entry and exit channels of the ribosomal preinitiation complex

Colin Echeverría Aitken, A. Hinnebusch, J. Lorsch, 2016, bioRxiv.

Development and characterization of a reconstituted yeast translation initiation system.

Mikkel A. Algire, A. Hinnebusch, A. Sachs, 2002, RNA.

Multiple elements in the eIF4G1 N‐terminus promote assembly of eIF4G1•PABP mRNPs in vivo

Sarah E. Walker, A. Hinnebusch, J. Lorsch, 2011, The EMBO journal.

Genetic identification of yeast 18S rRNA residues required for efficient recruitment of initiator tRNA(Met) and AUG selection.

A. Hinnebusch, J. Lorsch, B. Shin, 2008, Genes & development.

Recruiting knotty partners: The roles of translation initiation factors in mRNA recruitment to the eukaryotic ribosome

Colin Echeverría Aitken, J. Lorsch, V. Rajagopal, 2011 .

The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to stimulate canonical translation initiation and to block an alternative pathway.

Sarah E. Walker, Mikkel A. Algire, A. Hinnebusch, 2010, Molecular cell.

Perspective: Sustaining the big-data ecosystem

Jon R Lorsch, Philip E Bourne, Eric D Green, 2015, Nature.

The mercury resistance (mer) operon in a marine gliding flavobacterium, Tenacibaculum discolor 9A5.

J. Lorsch, J. Mccauley, J. Voet, 2013, FEMS microbiology ecology.

The DEAD box protein eIF4A. 1. A minimal kinetic and thermodynamic framework reveals coupled binding of RNA and nucleotide.

D. Herschlag, J. Lorsch, 1998, Biochemistry.

Yeast Eukaryotic Initiation Factor 4B (eIF4B) Enhances Complex Assembly between eIF4A and eIF4G in Vivo*

Sarah E. Walker, A. Hinnebusch, J. Lorsch, 2012, The Journal of Biological Chemistry.

Fixing problems with cell lines

Francis S. Collins, Jennifer Lippincott-Schwartz, Jon R. Lorsch, 2014, Science.

Kinetic and thermodynamic analysis of the role of start codon/anticodon base pairing during eukaryotic translation initiation.

J. Lorsch, S. Kolitz, Julie E Takacs, 2008, RNA.

Yeast initiator tRNA identity elements cooperate to influence multiple steps of translation initiation.

J. Lorsch, S. Kolitz, Lee D. Kapp, 2006, RNA.

Labeling a protein with fluorophores using NHS ester derivitization.

J. Lorsch, J. Nanda, 2014, Methods in enzymology.

Identification of compounds that decrease the fidelity of start codon recognition by the eukaryotic translational machinery.

Nicholas T. Ingolia, A. Hinnebusch, J. Pelletier, 2011, RNA.

Yeast eIF4A enhances recruitment of mRNAs regardless of their structural complexity

Colin Echeverría Aitken, A. Hinnebusch, J. Lorsch, 2017, eLife.

Yeast eIF4B binds to the head of the 40S ribosomal subunit and promotes mRNA recruitment through its N-terminal and internal repeat domains.

Sarah E. Walker, A. Hinnebusch, J. Lorsch, 2013, RNA.

Ribozyme catalysis: not different, just worse

Jon R Lorsch, J. Doudna, J. Lorsch, 2005, Nature Structural &Molecular Biology.

Initiation of protein synthesis by hepatitis C virus is refractory to reduced eIF2.GTP.Met-tRNA(i)(Met) ternary complex availability.

J. Pelletier, J. Lorsch, R. Kaufman, 2006, Molecular biology of the cell.

Basic science: Bedrock of progress.

Christopher P Austin, Andrea Norris, William T Riley, 2016, Science.

Catalyzing the Modernization of Graduate Education: A major overhaul of how we educate graduate students in biomedical research is long overdue

J. Lorsch, A. Gammie, Shiva Singh, 2016 .

Practical steady-state enzyme kinetics.

J. Lorsch, 2014, Methods in enzymology.

Protein Affinity Purification using Intein/Chitin Binding Protein Tags.

J. Lorsch, Sarah F. Mitchell, 2015, Methods in enzymology.

Translation initiation : cell biology, high-throughput methods, and chemical-based approaches

Jon Lorsch, J. Lorsch, 2007 .

Standard in vitro assays for protein-nucleic acid interactions--gel shift assays for RNA and DNA binding.

J. Lorsch, Sarah F. Mitchell, 2014, Methods in enzymology.

Yeast Ded1 promotes 48S translation pre-initiation complex assembly in an mRNA-specific and eIF4F-dependent manner

A. Hinnebusch, J. Lorsch, Neha Gupta, 2018, bioRxiv.

Developing a culture of safety in biomedical research training

J. Lorsch, A. Gammie, Michelle Bond, 2020, Molecular biology of the cell.

Mechanism of Ribosomal Peptide Bond Formation

J. Berg, J. Lorsch, 2001, Science.

Yeast eIF4A enhances recruitment of mRNAs regardless of their structural complexity

Colin Echeverría Aitken, A. Hinnebusch, J. Lorsch, 2017, eLife.

Eukaryotic initiator tRNA: Finely tuned and ready for action

J. Lorsch, Sarah E. Kolitz, Jon R. Lorsch, 2010, FEBS letters.

The eIF1A C‐terminal domain promotes initiation complex assembly, scanning and AUG selection in vivo

A. Hinnebusch, J. Lorsch, T. Pestova, 2005, The EMBO journal.

Multiple elements in the eIF4G1 N‐terminus promote assembly of eIF4G1•PABP mRNPs in vivo

Sarah E. Walker, A. Hinnebusch, J. Lorsch, 2011, The EMBO journal.

Sanger dideoxy sequencing of DNA.

Jon Lorsch, Sarah E Walker, Sarah E. Walker, 2013, Methods in enzymology.

Maximizing the return on taxpayers' investments in fundamental biomedical research

Jon R. Lorsch, J. Lorsch, 2015, Molecular biology of the cell.

The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosome.

Mikkel A. Algire, V. Ramakrishnan, J. Lorsch, 2007, Molecular cell.

Structure of a partial yeast 48S preinitiation complex with eIF5 N-terminal domain (model C1)

A. Hinnebusch, V. Ramakrishnan, J. Lorsch, 2018 .

Basic science: Bedrock of progress.

Elizabeth L. Wilder, Franziska B Greider, N. Volkow, 2016, Science.