P. Alves

Manuel J T Carrondo, Paula M Alves, Cristina Peixoto, 2008, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Evaluation of helper-dependent canine adenovirus 1 vectors in a 3 D human CNS model 2 3

Giampietro, C. Peddie, L. Collinson, 2015 .

Clinical-grade Oncolytic Adenovirus Purification using Polysorbate 20 as an Alternative for Cell Lysis

P. Alves, M. Carrondo, C. Peixoto, 2018, Current gene therapy.

Extending hepatocyte functionality for drug-testing applications using high-viscosity alginate-encapsulated three-dimensional cultures in bioreactors.

P. Alves, M. Carrondo, S. Leite, 2010, Tissue engineering. Part C, Methods.

Resource Recapitulation of Human Neural Microenvironment Signatures in iPSC-Derived NPC 3 D Differentiation

Marta M. Silva, P. Alves, M. Sousa, 2018 .

Biolayer Interferometry Analysis for a Higher Throughput Quantification of In-Process Samples of a Rotavirus Vaccine

Sofia B. Carvalho, Sofia B Carvalho, P. Alves, 2022, Vaccines.

Bioreactor-based 3D human myocardial ischemia/reperfusion in vitro model: a novel tool to unveil key paracrine factors upon AMI.

P. Alves, M. Serra, P. Gomes-Alves, 2019, Translational research : the journal of laboratory and clinical medicine.

Gene Expression Analysis of Adapted Insect Cells during Influenza VLP Production Using RNA-Sequencing

Inês A. Isidro, C. Clarke, P. Alves, 2022, Viruses.

Canine helper-dependent vectors production: implications of Cre activity and co-infection on adenovirus propagation

P. Alves, A. S. Coroadinha, E. Kremer, 2015, Scientific Reports.

Translation of liver stage activity of M5717, a Plasmodium elongation factor 2 inhibitor: from bench to bedside

Akash Khandelwal, P. Alves, Ö. Yalkinoglu, 2022, Malaria journal.

Quantification of Ehrlichia ruminantium by real time PCR.

P. Alves, N. Vachiéry, D. Martinez, 2005, Veterinary microbiology.

Expression of Extracellular Vesicle PIWI-Interacting RNAs Throughout hiPSC-Cardiomyocyte Differentiation

Inês A. Isidro, P. Alves, M. Serra, 2022, Frontiers in Physiology.

Expression of recombinant antibody and secreted alkaline phosphatase in mammalian cells. Influence of cell line and culture system upon production kinetics

J. B. Griffiths, P. Alves, M. Carrondo, 1994, Applied Microbiology and Biotechnology.

Relationship between retroviral vector membrane and vector stability.

P. Cruz, P. Alves, M. Carrondo, 2006, The Journal of general virology.

1H-NMR spectroscopy for human 3D neural stem cell cultures metabolic profiling

Ana P. Teixeira, A. Teixeira, P. Alves, 2013, BMC Proceedings.

H-nmr Spectroscopy for Human 3d Neural Stem Cell Cultures Metabolic Profiling Materials and Methods H-nmr Spectra Acquisition and Profiling

A. Teixeira, P. Alves, D. Simão, 2022 .

Bioanalytics for Influenza Virus-Like Particle Characterization and Process Monitoring

Sofia B. Carvalho, Sofia B Carvalho, P. Alves, 2022, Frontiers in Bioengineering and Biotechnology.

Modeling protein binding and elution over a chromatographic surface probed by surface plasmon resonance.

P. Alves, J. Mota, M. Carrondo, 2010, Journal of chromatography. A.

Metabolic changes during cell growth inhibition by the IRF-1 system

P. Alves, M. Carrondo, H. Hauser, 2002 .

Impact of E1 and Cre on Adenovirus Vector Amplification: Developing MDCK CAV-2-E1 and E1-Cre Transcomplementing Cell Lines

P. Alves, A. S. Coroadinha, E. Kremer, 2013, PloS one.

Stabilization of gammaretroviral and lentiviral vectors: from production to gene transfer

P. Cruz, P. Alves, M. Carrondo, 2009, The journal of gene medicine.

A detection and quantification label-free tool to speed up downstream processing of model mucins

Sofia B. Carvalho, Sofia B Carvalho, P. Alves, 2018, PloS one.

Stirred vessel cultures of rat brain cells aggregates: Characterization of major metabolic pathways and cell population dynamics

P. Alves, M. Carrondo, U. Sonnewald, 2007, Journal of neuroscience research.

Process development for the mass production of Ehrlichia ruminantium.

P. Alves, A. Cunha, M. Sousa, 2006, Vaccine.

Improving retroviral vectors production: role of carbon sources in lipid biosynthesis.

P. Alves, A. I. Amaral, A. S. Coroadinha, 2008, Journal of biotechnology.

Flexible 3D cell-based platforms for the discovery and profiling of novel drugs targeting Plasmodium hepatic infection.

P. Alves, M. Carrondo, D. Simão, 2019, ACS infectious diseases.

Production of high‐quality SARS‐CoV‐2 antigens: Impact of bioprocess and storage on glycosylation, biophysical attributes, and ELISA serologic tests performance

J. Demengeot, M. Bergman, P. Alves, 2020, Biotechnology and bioengineering.

Rational development of two flowthrough purification strategies for adenovirus type 5 and retro virus-like particles.

P. Alves, J. Mota, M. Carrondo, 2015, Journal of chromatography. A.

Carboxylesterase 2 production and characterization in human cells: new insights into enzyme oligomerization and activity

P. Alves, M. Sousa, A. S. Coroadinha, 2012, Applied Microbiology and Biotechnology.

Screening of Novel Excipients for Improving the Stability of Retroviral and Adenoviral Vectors

P. Cruz, P. Alves, M. Carrondo, 2006, Biotechnology progress.

Establishing Suspension Cell Cultures for Improved Manufacturing of Oncolytic Adenovirus.

P. Alves, M. Sousa, M. Carrondo, 2020, Biotechnology journal.

Intensifying Continuous Production of Gag-HA VLPs at High Cell Density Using Stable Insect Cells Adapted to Low Culture Temperature

P. Alves, A. Roldão, Bárbara Fernandes, 2022, Frontiers in Bioengineering and Biotechnology.

A Flow-Through Chromatographic Strategy for Hepatitis C Virus-Like Particles Purification

P. Alves, M. Carrondo, C. Peixoto, 2020, Processes.

In Vitro Approaches for Improved Rotavirus VLP’s Quality

P. Alves, M. Carrondo, M. Mellado, 2012 .

Portuguese winemaking residues as a potential source of natural anti-adenoviral agents

P. Alves, T. Ferreira, A. Matias, 2010, International journal of food sciences and nutrition.

Impact of physicochemical parameters on in vitro assembly and disassembly kinetics of recombinant triple‐layered rotavirus‐like particles

Manuel J T Carrondo, Paula M Alves, António Lopes, 2009, Biotechnology and bioengineering.

Polymethoxylated Flavones from Orange Peels Inhibit Cell Proliferation in a 3D Cell Model of Human Colorectal Cancer

P. Alves, M. Bronze, A. Serra, 2018, Nutrition and cancer.

Downstream processing of triple layered rotavirus like particles.

P M Alves, M J T Carrondo, P. Alves, 2007, Journal of biotechnology.

Tetraspanins displayed in retrovirus-derived virus-like particles and their immunogenicity.

D. Klatzmann, P. Alves, M. Carrondo, 2016, Vaccine.

Strategies for improved stability of Peste des Petits Ruminants Vaccine.

P. Alves, M. Carrondo, A. Silva, 2011, Vaccine.

Surface‐based cryopreservation strategies for human embryonic stem cells: A comparative study

J. C. Schulz, P. Alves, H. Zimmermann, 2012, Biotechnology progress.

Targeted supplementation design for improved production and quality of enveloped viral particles in insect cell-baculovirus expression system.

V. Bernal, P. Alves, I. Berger, 2016 .

Use of adenoviral vectors as veterinary vaccines

P. Alves, M. Carrondo, J. Auniņš, 2005, Gene Therapy.

Baculovirus production for gene therapy: the role of cell density, multiplicity of infection and medium exchange

Rui Oliveira, Vicente Bernal, Paula M. Alves, 2009, Applied Microbiology and Biotechnology.

Improvement of recombinant protein production by an anti-apoptotic protein from hemolymph of Lonomia obliqua

P. Alves, H. Vieira, C. Peixoto, 2010, Cytotechnology.

Intracellular dynamics in rotavirus-like particles production: Evaluation of multigene and monocistronic infection strategies

Rui Oliveira, Paula M. Alves, António Roldão, 2006 .

Proteolytic activity in infected and noninfected insect cells: degradation of HIV-1 Pr55gag particles.

P. Cruz, P. Alves, M. Carrondo, 1999, Biotechnology and bioengineering.

Cell density effect in the baculovirus‐insect cells system: A quantitative analysis of energetic metabolism

Vicente Bernal, Paula M Alves, Nuno Carinhas, 2009, Biotechnology and bioengineering.

Human neuron-astrocyte 3D co-culture-based assay for evaluation of neuroprotective compounds.

P. Alves, C. Brito, A. P. Terrasso, 2017, Journal of pharmacological and toxicological methods.

Functional metabolic interactions of human neuron-astrocyte 3D in vitro networks

Ana P. Teixeira, A. Teixeira, P. Alves, 2016, Scientific Reports.

Novel scalable 3D cell based model for in vitro neurotoxicity testing: Combining human differentiated neurospheres with gene expression and functional endpoints.

Catarina Brito, Margarida Serra, Paula Marques Alves, 2015, Journal of biotechnology.

A multi-organ chip co-culture of neurospheres and liver equivalents for long-term substance testing.

Uwe Marx, Catarina Brito, Eva-Maria Materne, 2015, Journal of biotechnology.

Combining Hypoxia and Bioreactor Hydrodynamics Boosts Induced Pluripotent Stem Cell Differentiation Towards Cardiomyocytes

P. Alves, T. Šarić, M. Sousa, 2014, Stem Cell Reviews and Reports.

Production and purification of functional truncated soluble forms of human recombinant L1 cell adhesion glycoprotein from Spodoptera frugiperda Sf9 cells.

P. Alves, V. Morais, C. Peixoto, 2007, Protein expression and purification.

Cultures of rat astrocytes challenged with a steady supply of glutamate: New model to study flux distribution in the glutamate–glutamine cycle

P. Alves, M. Carrondo, P. Alves, 2005, Glia.

Towards purification of adenoviral vectors based on membrane technology

P. Alves, M. Carrondo, C. Peixoto, 2008, Biotechnology progress.

Viruses and Virus-Like Particles in Biotechnology: Fundamentals and Applications

António Roldão, Ana Carina Silva, Mcm Mellado, 2017, Comprehensive Biotechnology.

Modeling human neural functionality in vitro: three-dimensional culture for dopaminergic differentiation.

Ana P. Teixeira, P. A. Lima, C. Peddie, 2015, Tissue engineering. Part A.

A novel asexual blood-stage malaria vaccine candidate: PfRipr5 formulated with human-use adjuvants induces potent growth inhibitory antibodies

P. Alves, E. Takashima, T. Tsuboi, 2022, Frontiers in Immunology.

Viral vaccines and their manufacturing cell substrates: New trends and designs in modern vaccinology

P. Alves, A. S. Coroadinha, M. R. Guerreiro, 2015, Biotechnology journal.

Enhancing the purification of Lentiviral vectors for clinical applications

Mary Collins, Paula M. Alves, Cristina Peixoto, 2021 .

Bioengineered Liver Cell Models of Hepatotropic Infections

P. Alves, C. Brito, F. Arez, 2021, Viruses.

Next generation of heart regenerative therapies: progress and promise of cardiac tissue engineering

P. Alves, M. Tenreiro, M. Serra, 2021, NPJ Regenerative medicine.

Metabolic Maturation of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Inhibition of HIF1&agr; and LDHA

P. Alves, D. Milan, A. Garakani, 2018, Circulation research.

Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells.

P. Alves, M. Sousa, D. Simão, 2016, Methods in molecular biology.

Adenovirus vector production and purification.

S. Kochanek, P. Cruz, P. Alves, 2010, Current gene therapy.

Transcriptome analysis of Sf9 insect cells during production of recombinant Adeno‐associated virus

Inês A. Isidro, C. Clarke, P. Alves, 2022, Biotechnology journal.

3D-3-culture: A tool to unveil macrophage plasticity in the tumour microenvironment.

W. Sommergruber, P. Alves, P. Loza-Álvarez, 2018, Biomaterials.

Modelling the tumour microenvironment in long-term microencapsulated 3D co-cultures recapitulates phenotypic features of disease progression.

Simon T Barry, Catarina Brito, Paula M Alves, 2016, Biomaterials.

Hallmarks of the human intestinal microbiome on liver maturation and function.

Pedro M. Baptista, J. Gisbert, P. Alves, 2021, Journal of hepatology.

Distinct carbon sources affect structural and functional maturation of cardiomyocytes derived from human pluripotent stem cells

Ana P. Teixeira, A. Teixeira, P. Alves, 2017, Scientific Reports.

Flexible pseudotyping of retrovirus using recombinase-mediated cassette exchange

P. Alves, A. S. Coroadinha, H. Soares, 2018, Biotechnology Letters.

Down-regulation of CD81 in human cells producing HCV-E1/E2 retroVLPs

D. Klatzmann, P. Alves, M. Carrondo, 2011, BMC proceedings.

Down-regulation of CD 81 in human cells producing HCV-E 1 / E 2 retroVLPs

D. Klatzmann, P. Alves, M. Carrondo, 2011 .

Lentiviral Gene Therapy Vectors: Challenges and Future Directions

P. Alves, A. S. Coroadinha, H. Tomás, 2013 .

Quantitation of MLV-based retroviral vectors using real-time RT-PCR.

P. Cruz, P. Alves, M. Carrondo, 2004, Journal of virological methods.

Drug screening in 3D in vitro tumor models: overcoming current pitfalls of efficacy read‐outs

Catarina Brito, Paula M Alves, Vítor E Santo, 2017, Biotechnology journal.

Thermosensitivity of the reverse transcription process as an inactivation mechanism of lentiviral vectors.

P. Cruz, P. Alves, M. Carrondo, 2009, Human gene therapy.

Detection and Quantification of Label-Free Infectious Adenovirus Using a Switch-On Cell-Based Fluorescent Biosensor.

P. Alves, A. S. Coroadinha, M. R. Guerreiro, 2019, ACS sensors.

Advances in Lentivirus Purification

P. Alves, M. Carrondo, C. Peixoto, 2020, Biotechnology journal.

From retroviral vector production to gene transfer: spontaneous inactivation is caused by loss of reverse transcription capacity

P. Cruz, P. Alves, M. Carrondo, 2008, The journal of gene medicine.

Enhancing Hepatitis C Virus pseudoparticles infectivity through p7NS2 cellular expression.

P. Alves, A. S. Coroadinha, H. Soares, 2019, Journal of virological methods.

Metabolic pathways recruited in the production of a recombinant enveloped virus: mining targets for process and cell engineering.

P. Alves, A. S. Coroadinha, V. Bandeira, 2013, Metabolic engineering.

Retroviral vector production under serum deprivation: The role of lipids

P. Alves, A. S. Coroadinha, M. Carmo, 2009, Biotechnology and bioengineering.

Metabolic shifts do not influence the glycosylation patterns of a recombinant fusion protein expressed in BHK cells.

P M Alves, H J Cruz, P. Alves, 2000, Biotechnology and bioengineering.

Expansion of functional personalized cells with specific transgene combinations

Q. Yuan, M. Ott, J. Riancho, 2018, Nature Communications.

Conversion of Nonproliferating Astrocytes into Neurogenic Neural Stem Cells: Control by FGF2 and Interferon‐γ

E. Glaab, A. Teixeira, P. Alves, 2016, Stem cells.

Triple layered rotavirus VLP production: kinetics of vector replication, mRNA stability and recombinant protein production.

Paula M Alves, António Roldão, Manuel J T Carrondo, 2005, Journal of biotechnology.

Cryopreservation in micro‐volumes: Impact upon caco‐2 colon adenocarcinoma cell proliferation and differentiation

P. Alves, M. Carrondo, H. Zimmermann, 2007, Biotechnology and bioengineering.

Upstream Bioprocess for Adenovirus Vectors

Paula M. Alves, Paulo Jorge Fernandes, Ana Carina Silva, 2016 .

Evaluation of Novel Large Cut-Off Ultrafiltration Membranes for Adenovirus Serotype 5 (Ad5) Concentration

P. Alves, J. Mota, M. Carrondo, 2014, PloS one.

Purification of adenoviral vectors using expanded bed chromatography.

P M Alves, P. Cruz, P. Alves, 2006, Journal of virological methods.

Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization

F. Fernández‐Avilés, B. Ibáñez, P. Alves, 2019, Scientific Reports.

Dielectric Spectroscopy to Improve the Production of rAAV Used in Gene Therapy

Inês A. Isidro, Peter G. Slade, P. Alves, 2020, Processes.

Perfusion of 3D encapsulated hepatocytes—A synergistic effect enhancing long‐term functionality in bioreactors

P. Alves, M. Sousa, M. Carrondo, 2011, Biotechnology and bioengineering.

Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater.

P. Alves, A. I. Amaral, N. Vachiéry, 2007, Vaccine.

Insect High Five™ cell line development using site-specific flipase recombination technology

A. Teixeira, P. Alves, M. M. Dias, 2021, G3.

AAV process intensification by perfusion bioreaction and integrated clarification

P. Alves, A. Roldão, C. Peixoto, 2022, Frontiers in Bioengineering and Biotechnology.

Adaptable stirred-tank culture strategies for large scale production of multicellular spheroid-based tumor cell models.

Catarina Brito, Paula M. Alves, Vítor E. Santo, 2016, Journal of biotechnology.

Proteomic and Glyco(proteo)mic tools in the profiling of cardiac progenitors and pluripotent stem cell derived cardiomyocytes: Accelerating translation into therapy.

P. Alves, P. Gomes-Alves, Lara Marcos-Silva, 2021, Biotechnology advances.

Systems biotechnology of animal cells: the road to prediction.

Rui Oliveira, Ana P Teixeira, Nuno Carinhas, 2012, Trends in biotechnology.

Synchronous fluorescence spectroscopy as a novel tool to enable PAT applications in bioprocesses

Paula M Alves, Ana P Teixeira, Ana P. Teixeira, 2011, Biotechnology and bioengineering.

Advances in on-line monitoring and control of mammalian cell cultures: Supporting the PAT initiative.

Ana P. Teixeira, A P Teixeira, R Oliveira, 2009, Biotechnology advances.

Validation of Bioreactor and Human-on-a-Chip Devices for Chemical Safety Assessment.

Uwe Marx, Catarina Brito, Paula M Alves, 2016, Advances in experimental medicine and biology.

Two Different Serum-free Media and Osmolality Effect Upon Human 293 Cell Growth and Adenovirus Production

P. Alves, M. Carrondo, T. Ferreira, 2005, Biotechnology Letters.

Testing a new formulation for Peste des Petits Ruminants vaccine in Ethiopia.

P. Alves, M. Carrondo, A. Silva, 2014, Vaccine.

Effect of ammonia production on intracellular pH: Consequent effect on adenovirus vector production.

P. Alves, M. Carrondo, T. Ferreira, 2007, Journal of biotechnology.

293 cell cycle synchronisation adenovirus vector production

P. Alves, J. Auniņš, M. Carrondo, 2009, Biotechnology progress.

Scalable culture systems using different cell lines for the production of Peste des Petits ruminants vaccine.

P. Alves, M. Sousa, M. Carrondo, 2008, Vaccine.

The importance of 293 cell cycle phase on adenovirus vector production

M. Haury, P. Cruz, P. Alves, 2006 .

Novel scFv against Notch Ligand JAG1 Suitable for Development of Cell Therapies toward JAG1-Positive Tumors

P. Alves, D. Simão, Gabriela Silva, 2023, Biomolecules.

Human cardiac stem cells inhibit lymphocyte proliferation through paracrine mechanisms that correlate with indoleamine 2,3-dioxygenase induction and activity

P. Alves, E. Lombardo, M. Serra, 2018, Stem Cell Research & Therapy.

Single-step cloning-screening method: a new tool for developing and studying high-titer viral vector producer cells

P. Alves, A. S. Coroadinha, M. R. Guerreiro, 2015, Gene Therapy.

Modeling electrostatic interactions of baculovirus vectors for ion-exchange process development.

P. Alves, M. Carrondo, T. Vicente, 2010, Journal of chromatography. A.

Modeling rotavirus-like particles production in a baculovirus expression vector system: Infection kinetics, baculovirus DNA replication, mRNA synthesis and protein production.

Manuel J T Carrondo, Paula M Alves, António Roldão, 2007, Journal of biotechnology.

Impact of grafting on the design of new membrane adsorbers for adenovirus purification.

P. Alves, J. Mota, M. Carrondo, 2014, Journal of biotechnology.

Quantitative Proteomics of Spodoptera frugiperda Cells during Growth and Baculovirus Infection

Rui Oliveira, Nuno Carinhas, Paula Marques Alves, 2011, PloS one.

HepaRG microencapsulated spheroids in DMSO-free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism

P. Alves, C. Brito, V. Shevchenko, 2015, Archives of Toxicology.

Population homogeneity for the antibody response to COVID-19 BNT162b2/Comirnaty vaccine is only reached after the second dose across all adult age ranges

J. Demengeot, M. Bergman, P. Alves, 2022, Nature communications.

Extracellular Vesicles from Ovarian Carcinoma Cells Display Specific Glycosignatures

Sofia B. Carvalho, Sofia B Carvalho, P. Altevogt, 2015, Biomolecules.

An integrated analysis of enzyme activities, cofactor pools and metabolic fluxes in baculovirus-infected Spodoptera frugiperda Sf9 cells.

Vicente Bernal, Paula M Alves, Nuno Carinhas, 2010, Journal of biotechnology.

Exosome-based therapeutics: Purification using semi-continuous multi-column chromatography

P. Alves, M. Carrondo, C. Peixoto, 2019, Separation and Purification Technology.

Efficiency of inactivated vaccines against heartwater in Burkina Faso: impact of Ehrlichia ruminantium genetic diversity.

P. Alves, D. Meyer, N. Vachiéry, 2010, Vaccine.

Immunisation of sheep against heartwater in The Gambia using inactivated and attenuated Ehrlichia ruminantium vaccines.

P. Alves, L. Bell-Sakyi, F. Jongejan, 2007, Vaccine.

Development of a Vaccine Candidate Against Heartwater

P. Alves, A. I. Amaral, M. Sousa, 2010 .

Process Development for a Veterinary Vaccine Against Heartwater Using Stirred Tanks

P. Alves, A. Cunha, A. I. Amaral, 2007 .

Process Development for a Veterinary Vaccine against Heartwater

P. Alves, M. Sousa, M. Carrondo, 2005 .

Expansion of 3D human induced pluripotent stem cell aggregates in bioreactors: Bioprocess intensification and scaling-up approaches.

Margarida Serra, Paula M Alves, P. Alves, 2017, Journal of biotechnology.

Quantification of Metabolic Rearrangements During Neural Stem Cells Differentiation into Astrocytes by Metabolic Flux Analysis

Ana P. Teixeira, A. Teixeira, P. Alves, 2016, Neurochemical Research.

Improving washing strategies of human mesenchymal stem cells using negative mode expanded bed chromatography.

Margarida Serra, Manuel J T Carrondo, Paula M Alves, 2016, Journal of chromatography. A.

Characterization of Ehrlichia ruminantium replication and release kinetics in endothelial cell cultures.

P. Alves, M. Sousa, M. Carrondo, 2005, Veterinary microbiology.

Proteomic analyses of Ehrlichia ruminantium highlight differential expression of MAP1-family proteins.

P. Alves, D. Meyer, A. D. de Almeida, 2012, Veterinary microbiology.

Culture Methods for Mass Production of Ruminant Endothelial Cells

P. Alves, M. Carrondo, J. Moreira, 2003 .

Universal label-free in-process quantification of influenza virus-like particles.

Manuel J T Carrondo, Paula M Alves, René Gantier, 2017, Biotechnology journal.

Filtration methodologies for the clarification and concentration of human mesenchymal stem cells

Paula M. Alves, Margarida Serra, Cristina Peixoto, 2015 .

Flipase‐mediated cassette exchange in Sf9 insect cells for stable gene expression

A. Teixeira, P. Alves, K. Prather, 2012, Biotechnology and bioengineering.

CXCL6 is an important paracrine factor in the pro-angiogenic human cardiac progenitor-like cell secretome

P. Alves, A. Bernad, C. Torroja, 2017, Scientific Reports.

Conversion of non-proliferating astrocytes into neurogenic neural stem cells: control by FGF2 and IFN-gamma

E. Glaab, A. Teixeira, P. Alves, 2016 .

Production of complex virus-like particles in insect cells

A. Teixeira, Nuno Carinhas, P. Alves, 2014 .

3D-printed ordered bed structures for chromatographic purification of enveloped and non-enveloped viral particles

P. Alves, M. Carrondo, C. Peixoto, 2021 .

Adenovirus purification by two-column, size-exclusion, simulated countercurrent chromatography.

Manuel J T Carrondo, Paula M Alves, Cristina Peixoto, 2014, Journal of chromatography. A.

Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors

K. Airenne, P. Alves, M. Carrondo, 2011, Gene Therapy.

Rational design and optimization of downstream processes of virus particles for biopharmaceutical applications: current advances.

P. Alves, J. Mota, M. Carrondo, 2011, Biotechnology advances.

Dissecting insect cell heterogeneity during influenza VLP production using single-cell transcriptomics

Inês A. Isidro, C. Clarke, P. Alves, 2023, Frontiers in Bioengineering and Biotechnology.

Metabolic profiling of insect cell lines: Unveiling cell line determinants behind system's productivity

V. Bernal, P. Alves, M. Carrondo, 2014, Biotechnology and bioengineering.

Unveiling dynamic metabolic signatures in human induced pluripotent and neural stem cells

João Vasconcelos e Sá, Daniel Simão, Ana P. Terrasso, 2020, PLoS Comput. Biol..

Human cardiac progenitor cell activation and regeneration mechanisms: exploring a novel myocardial ischemia/reperfusion in vitro model

P. Alves, M. Serra, P. Gomes-Alves, 2019, Stem cell research & therapy.

Monitoring virus-like particle and viral protein production by intact cell MALDI-TOF mass spectrometry.

P. Alves, A. Coelho, C. Franco, 2010, Talanta.

Metabolic signatures of GS‐CHO cell clones associated with butyrate treatment and culture phase transition

Paula M Alves, Ana P Teixeira, Nuno Carinhas, 2013, Biotechnology and bioengineering.

Robust Expansion of Human Pluripotent Stem Cells: Integration of Bioprocess Design With Transcriptomic and Metabolomic Characterization

Marta M. Silva, P. Alves, A. S. Coroadinha, 2015, Stem cells translational medicine.

Hybrid metabolic flux analysis: combining stoichiometric and statistical constraints to model the formation of complex recombinant products

Rui Oliveira, Vicente Bernal, Paula M. Alves, 2011, BMC Systems Biology.

Culturing primary brain astrocytes under a fully controlled environment in a novel bioreactor

P. Alves, M. Carrondo, S. S. Santos, 2005, Journal of neuroscience research.

Holographic Imaging of Insect Cell Cultures: Online Non-Invasive Monitoring of Adeno-Associated Virus Production and Cell Concentration

P. Alves, D. Pais, J. Barbau, 2020, Processes.

Enabling PAT in insect cell bioprocesses: In situ monitoring of recombinant adeno‐associated virus production by fluorescence spectroscopy

Rui M. C. Portela, P. Alves, M. Carrondo, 2019, Biotechnology and bioengineering.

Efficient filtration strategies for the clarification of influenza virus-like particles derived from insect cells

Sofia B Carvalho, P. Alves, J. Clemente, 2019, Separation and Purification Technology.

Improved storage of influenza HA-VLPs using a trehalose-glycerol natural deep eutectic solvent system.

P. Alves, A. Duarte, M. Carrondo, 2021, Vaccine.

Improving Influenza HA-Vlps Production in Insect High Five Cells via Adaptive Laboratory Evolution

P. Alves, M. Carrondo, A. Roldão, 2020, Vaccines.

Effect of Growth Arrest in BHK Metabolism: On Line Monitoring by 13C and 31P NMR Spectroscopy

P. Alves, J. Moreira, H. Santos, 1998 .

Evaluation of helper-dependent canine adenovirus vectors in a 3D human CNS model

P M Alves, C. Peddie, L. Collinson, 2015, Gene Therapy.

Error assessment in recombinant baculovirus titration: evaluation of different methods.

Paula M. Alves, António Roldão, P. Alves, 2009, Journal of virological methods.

Human liver cell spheroids in extended perfusion bioreactor culture for repeated‐dose drug testing

Catarina Brito, Margarida Serra, Manuel J T Carrondo, 2012, Hepatology.

Metabolic Flux Analysis: A Powerful Tool in Animal Cell Culture

João V. Sá, Tiago M. Duarte, Manuel J.T. Carrondo, 2015 .

High-throughput analysis of animal cell cultures using two-dimensional fluorometry.

Rui Oliveira, Paula M. Alves, Tiago M. Duarte, 2011, Journal of biotechnology.

Online monitoring of hiPSC expansion and hepatic differentiation in 3D culture by dielectric spectroscopy

F. Prósper, P. Alves, M. Serra, 2021, Biotechnology and bioengineering.

In situ 2D fluorometry and chemometric monitoring of mammalian cell cultures

Rui Oliveira, Carla A M Portugal, Paula M Alves, 2009, Biotechnology and bioengineering.

Transcriptional Response of Human Neurospheres to Helper-Dependent CAV-2 Vectors Involves the Modulation of DNA Damage Response, Microtubule and Centromere Gene Groups

P. Alves, Carla Mottini, S. Salinas, 2015, PloS one.

Bioprocessing of Human Pluripotent Stem Cells for Cell Therapy Applications

Catarina Brito, Cláudia Correia, Margarida Serra, 2014 .

Towards real-time monitoring of therapeutic protein quality in mammalian cell processes.

Daniel A M Pais, Manuel J T Carrondo, Paula M Alves, 2014, Current opinion in biotechnology.

Cell Bank Origin of MDCK Parental Cells Shapes Adaptation to Serum-Free Suspension Culture and Canine Adenoviral Vector Production

P. Alves, A. S. Coroadinha, Y. Genzel, 2020, International journal of molecular sciences.

Adaptive laboratory evolution of stable insect cell lines for improved HIV-Gag VLPs production.

A. Teixeira, P. Alves, João Vidigal, 2019, Journal of biotechnology.

Novel culture strategy for human stem cell proliferation and neuronal differentiation

P. Alves, M. Carrondo, C. Brito, 2007, Journal of neuroscience research.

Production of canine adenovirus type 2 in serum-free suspension cultures of MDCK cells

P. Alves, M. Sousa, A. S. Coroadinha, 2015, Applied Microbiology and Biotechnology.

Population homogeneity for the antibody response to COVID-19 Comirnaty vaccine is only reached after the second dose

J. Demengeot, M. Bergman, P. Alves, 2021, medRxiv.

Modelling and optimization of a recombinant BHK-21 cultivation process using hybrid grey-box systems.

Ana P. Teixeira, P M Alves, M J T Carrondo, 2005, Journal of biotechnology.

Bioorthogonal Strategy for Bioprocessing of Specific-Site-Functionalized Enveloped Influenza-Virus-Like Particles

Sofia B Carvalho, P. Alves, M. Carrondo, 2016, Bioconjugate chemistry.

On the Effect of Thermodynamic Equilibrium on the Assembly Efficiency of Complex Multi-Layered Virus-Like Particles (VLP): the Case of Rotavirus VLP

Rui Oliveira, Paula M. Alves, Maria Candida M. Mellado, 2012, PLoS Comput. Biol..

Oncolytic virus purification with periodic counter‐current chromatography

P. Alves, M. Carrondo, C. Peixoto, 2021, Biotechnology and bioengineering.

Improving the downstream processing of vaccine and gene therapy vectors with continuous chromatography

Cristina Peixoto, Paula M. Alves, Manuel J.T. Carrondo, 2015 .

Robust design of adenovirus purification by two-column, simulated moving-bed, size-exclusion chromatography.

Cristina Peixoto, Piergiuseppe Nestola, P. Alves, 2015, Journal of biotechnology.

Palmitoylation Strengthens Cholesterol-dependent Multimerization and Fusion Activity of Human Cytomegalovirus Glycoprotein B (gB)*

A. Teixeira, P. Alves, A. S. Coroadinha, 2015, The Journal of Biological Chemistry.

Online Control Strategies

Rui Oliveira, Paula M. Alves, Ana P. Teixeira, 2019, Comprehensive Biotechnology.

Three‐dimensional co‐culture of human hepatocytes and mesenchymal stem cells: improved functionality in long‐term bioreactor cultures

Marta M. Silva, P. Alves, C. Brito, 2017, Journal of tissue engineering and regenerative medicine.

Combining metabolic flux analysis tools and 13C NMR to estimate intracellular fluxes of cultured astrocytes

Rui Oliveira, Paula M. Alves, Nuno Carinhas, 2008, Neurochemistry International.

A Comprehensive Metabolic Profile of Cultured Astrocytes Using Isotopic Transient Metabolic Flux Analysis and 13C-Labeled Glucose

Ana P. Teixeira, A. Teixeira, P. Alves, 2011, Front. Neuroenerg..

European Society for Animal Cell Technology

P. Alves, Y. Schneider, A. Roldão, 2004, Cytotechnology.

Production of rotavirus core-like particles in Sf9 cells using recombinase-mediated cassette exchange.

A. Teixeira, P. Alves, M. Sousa, 2014, Journal of biotechnology.

RMCE-based insect cell platform to produce membrane proteins captured on HIV-1 Gag virus-like particles

Ana P. Teixeira, A. Teixeira, P. Alves, 2017, Applied Microbiology and Biotechnology.

3D aggregate culture improves metabolic maturation of human pluripotent stem cell derived cardiomyocytes

Ana P. Teixeira, D. Elliott, A. Teixeira, 2018, Biotechnology and bioengineering.

Integrating high cell density cultures with adapted laboratory evolution for improved Gag‐HA virus‐like particles production in stable insect cell lines

P. Alves, M. Sousa, M. Carrondo, 2021, Biotechnology and bioengineering.

How can measurement, monitoring, modeling and control advance cell culture in industrial biotechnology?

Udo Reichl, Carl-Fredrik Mandenius, Rui Oliveira, 2012, Biotechnology journal.

Designing clinical-grade integrated strategies for the downstream processing of human mesenchymal stem cells

Marta M. Silva, P. Alves, M. Carrondo, 2013, BMC Proceedings.

Asexual Blood-Stage Malaria Vaccine Candidate PfRipr5: Enhanced Production in Insect Cells

P. Alves, E. Takashima, T. Tsuboi, 2022, Frontiers in Bioengineering and Biotechnology.

Hybrid elementary flux analysis/nonparametric modeling: application for bioprocess control

Rui Oliveira, Paula M. Alves, Manuel J. T. Carrondo, 2007, BMC Bioinformatics.

Removal of envelope protein-free retroviral vectors by anion-exchange chromatography to improve product quality.

P. Cruz, P. Alves, M. Carrondo, 2008, Journal of separation science.

Effect of Vitamins or Lipids Addition on Adenovirus Production at High Cell Densities

P. Alves, M. Carrondo, T. Ferreira, 2007 .

Translation of liver stage activity of M5717, a Plasmodium elongation factor 2 inhibitor: from bench to bedside

Akash Khandelwal, P. Alves, Ö. Yalkinoglu, 2022, Malaria journal.

Sodium dodecyl sulfate-capillary gel electrophoresis analysis of rotavirus-like particles.

Paula M Alves, P. Alves, C. Franco, 2008, Journal of chromatography. A.

3D-3-Culture: Tumor Models to Study Heterotypic Interactions in the Tumor Microenvironment

P. Alves, C. Brito, C. Pinto, 2020 .

Impact of dual‐baculovirus infection on the Sf9 insect cell transcriptome during rAAV production using single‐cell RNA‐seq

C. Clarke, P. Alves, A. Roldão, 2023, Biotechnology and bioengineering.

Advanced cell technologies: Making protein, cell, and gene therapies a reality

M. Betenbaugh, P. Alves, A. Roldão, 2023, Biotechnology and bioengineering.

Vaccine-induced neutralizing antibody responses to seasonal influenza virus H1N1 strains are not enhanced during subsequent pandemic H1N1 infection

P. Alves, M. V. van Gils, M. Carrondo, 2023, Frontiers in immunology.

Metabolic Flux Analysis Tools to Investigate Brain Metabolism In Vitro

A. Teixeira, P. Alves, A. I. Amaral, 2014 .

Generation and genetic modification of 3D cultures of human dopaminergic neurons derived from neural progenitor cells

P. Alves, J. Schwarz, E. Kremer, 2012 .

3D cultures of human neural progenitor cells: dopaminergic differentiation and genetic modification. [corrected].

P. Alves, J. Schwarz, E. Kremer, 2012, Methods.

Metabolic signatures of GS‐CHO cell clones associated with butyrate treatment and culture phase transition

A. Teixeira, Nuno Carinhas, P. Alves, 2013, Biotechnology and bioengineering.

Effects of ammonia and lactate on growth, metabolism, and productivity of BHK cells.

Alves, P. Alves, M. Carrondo, 2000, Enzyme and microbial technology.

Pre-erythrocytic Activity of M5717 in Monotherapy and Combination in Preclinical Plasmodium Infection Models

P. Alves, C. Brito, M. Prudêncio, 2022, ACS infectious diseases.

CXCL6 is an important paracrine factor in the pro-angiogenic human cardiac progenitor-like cell secretome

P. Alves, A. Bernad, C. Torroja, 2017, Scientific Reports.

Systems biotechnology of animal cells: the road to prediction.

Ana P. Teixeira, A. Teixeira, Nuno Carinhas, 2012, Trends in biotechnology.

Towards an extended functional hepatocyte in vitro culture.

P. Alves, M. Carrondo, S. Leite, 2009, Tissue engineering. Part C, Methods.

Improved virus purification processes for vaccines and gene therapy

P. Alves, M. Carrondo, C. Peixoto, 2015, Biotechnology and bioengineering.

Rotavirus-like particle production: Simulation of protein production and particle assembly

P. Alves, Rui Oliveira, M. Carrondo, 2006 .

Rational development of two flowthrough purification strategies for adenovirus type 5 and retro virus-like particles.

P. Alves, J. Mota, M. Carrondo, 2015, Journal of chromatography. A.

3D Cultures: Effect on the Hepatocytes Functionality

P. Alves, M. Carrondo, J. Miranda, 2010 .

Evaluation of the impact of matrix stiffness on encapsulated HepaRG spheroids

P. Alves, C. Brito, C. Chesné, 2013, BMC Proceedings.

Scalable culture systems using different cell lines for the production of Peste des Petits ruminants vaccine.

P. Alves, M. Sousa, M. Carrondo, 2008, Vaccine.

Towards human central nervous system in vitro models for preclinical research: strategies for 3D neural cell culture

P. Alves, J. Schwarz, D. Simão, 2011, BMC proceedings.

Adaptable stirred-tank culture strategies for large scale production of multicellular spheroid-based tumor cell models.

P. Alves, A. Serra, C. Brito, 2016, Journal of biotechnology.

Stochastic simulation of protein expression in the baculovirus/insect cells system

Rui Oliveira, Paula M. Alves, António Roldão, 2008, Comput. Chem. Eng..

Down‐regulation of CD81 tetraspanin in human cells producing retroviral‐based particles: Tailoring vector composition

D. Klatzmann, P. Alves, M. Carrondo, 2011, Biotechnology and bioengineering.

Hydrodynamic effects on BHK cells grown as suspended natural aggregates

P M Alves, P. Alves, J. Auniņš, 1995, Biotechnology and bioengineering.

Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cells.

P. Alves, M. Carrondo, A. S. Coroadinha, 2014, Trends in biotechnology.

Scalable Culture Strategies for the Expansion of Patient-Derived Cancer Stem Cell Lines

P. Alves, A. Serra, C. Brito, 2019, Stem cells international.