T. K. Christopoulos

发表

The biotin-(strept)avidin system: principles and applications in biotechnology.

E. Diamandis, E. P. Diamandis, T. K. Christopoulos, 1991, Clinical chemistry.

Microfabricated device for DNA and RNA amplification by continuous-flow polymerase chain reaction and reverse transcription-polymerase chain reaction with cycle number selection.

H. John Crabtree, Theodore K Christopoulos, Christopher J Backhouse, 2003, Analytical chemistry.

1 – PAST, PRESENT, AND FUTURE OF IMMUNOASSAYS

E. Diamandis, T. K. Christopoulos, 1996 .

14 – FLUORESCENCE IMMUNOASSAYS

T. K. Christopoulos, 1996 .

Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization.

T. K. Christopoulos, Monique Verhaegent, 2002, Analytical chemistry.

Continuous-flow DNA and RNA amplification chip combined with laser-induced fluorescence detection

Theodore K. Christopoulos, T. K. Christopoulos, P. Obeid, 2003 .

Determination of prostate specific antigen mRNA in peripheral blood by reverse transcriptase polymerase chain reaction and a simple chemiluminometric hybridization assay in a high-throughput format.

T. K. Christopoulos, Konstantinos Polizois, E. Emmanouilidou, 2003, Analytical biochemistry.

Quantitative reverse transcriptase-polymerase chain reaction for prostate-specific antigen mRNA.

T. K. Christopoulos, B. Galvan, 1997, Clinical biochemistry.

Detection of transgenes in soybean via a polymerase chain reaction and a simple bioluminometric assay based on a universal aequorin-labeled oligonucleotide probe

T. K. Christopoulos, P. Ioannou, K. Glynou, 2004, Analytical and bioanalytical chemistry.

Oligonucleotide-functionalized gold nanoparticles as probes in a dry-reagent strip biosensor for DNA analysis by hybridization.

Penelope C Ioannou, Theodore K Christopoulos, T. K. Christopoulos, 2003, Analytical chemistry.

Dual-allele dipstick assay for genotyping single nucleotide polymorphisms by primer extension reaction

T. K. Christopoulos, P. Ioannou, Jessica K Konstantou, 2009, European Journal of Human Genetics.

Visual genotyping of SNPs of drug-metabolizing enzymes by tetra-primer PCR coupled with a dry-reagent DNA biosensor.

T. K. Christopoulos, A. Gravanis, P. Ioannou, 2009, Pharmacogenomics.

Dipstick-type biosensor for visual detection of DNA with oligonucleotide-decorated colored polystyrene microspheres as reporters.

Despina P. Kalogianni, T. K. Christopoulos, P. Ioannou, 2009, Biosensors & bioelectronics.

Dry-reagent disposable dipstick test for visual screening of seven leukemia-related chromosomal translocations

Despina P. Kalogianni, T. K. Christopoulos, P. Ioannou, 2007, Nucleic acids research.

Photoprotein aequorin as a novel reporter for SNP genotyping by primer extension–application to the variants of mannose‐binding lectin gene

T. K. Christopoulos, E. Kanavakis, J. Traeger-Synodinos, 2006, Human mutation.

Multianalyte immunoassay based on spatially distinct fluorescent areas quantified by laser-excited solid-phase time-resolved fluorometry.

E. Diamandis, T. K. Christopoulos, S. Kakabakos, 1992, Clinical chemistry.

Two-round enzymatic amplification combined with time-resolved fluorometry of Tb3+ chelates for enhanced sensitivity in DNA hybridization assays.

T. K. Christopoulos, P. Ioannou, 1998, Analytical chemistry.

One-step purification and refolding of recombinant photoprotein aequorin by immobilized metal-ion affinity chromatography.

Penelope C Ioannou, Theodore K Christopoulos, T. K. Christopoulos, 2003, Protein expression and purification.

Quantitative bioluminometric method for DNA-based species/varietal identification in food authenticity assessment.

T. K. Christopoulos, G. Tucker, P. Kalaitzis, 2012, Journal of agricultural and food chemistry.

Screening for unknown mutations by a bioluminescent protein truncation test with homogeneous detection.

Despina P. Kalogianni, T. K. Christopoulos, Ioannis A. Trantakis, 2010, Journal of the American Chemical Society.

Quantitative polymerase chain reaction based on a dual-analyte chemiluminescence hybridization assay for target DNA and internal standard.

T. K. Christopoulos, M. Verhaegen, 1998, Analytical chemistry.

Enzyme-amplified aequorin-based bioluminometric hybridization assays.

T. K. Christopoulos, P. Ioannou, E. Laios, 2001, Analytical chemistry.

Mix-and-read method for assessment of milk pasteurization using a smartphone or a common digital camera

Sotirios S. Tragoulias, Despina P. Kalogianni, T. K. Christopoulos, 2020, Analytical and Bioanalytical Chemistry.

Dissolution studies of drug formulations using ion-selective electrodes as sensors in an air-segmented continuous flow analyzer.

E. Diamandis, M. Koupparis, T. K. Christopoulos, 1987, Journal of pharmaceutical sciences.

Expression immunoassay based on antibodies labeled with a deoxyribonucleic acid fragment encoding the α-peptide of β-galactosidase

T. K. Christopoulos, S. White, 1998 .

High-throughput chemiluminometric determination of prostate-specific membrane antigen mRNA in peripheral blood by RT-PCR using a synthetic RNA internal standard

T. K. Christopoulos, E. Emmanouilidou, P. Ioannou, 2004, Analytical and bioanalytical chemistry.

Quantification of prostate-specific antigen mRNA by coamplification with a recombinant RNA internal standard and microtiter well-based hybridization.

T. K. Christopoulos, P. Ioannou, M. Verhaegen, 1998, Clinical chemistry.

Identification of single-nucleotide polymorphisms by the oligonucleotide ligation reaction: a DNA biosensor for simultaneous visual detection of both alleles.

T. K. Christopoulos, C. Flordellis, P. Ioannou, 2009, Analytical chemistry.

Absolute quantification of the alleles in somatic point mutations by bioluminometric methods based on competitive polymerase chain reaction in the presence of a locked nucleic acid blocker or an allele-specific primer.

T. K. Christopoulos, J. Traeger-Synodinos, E. Kanavakis, 2011, Analytical chemistry.

Multi-allele dipstick assay for visual genotyping of four novel SIRT1 gene variant alleles as candidate biomarkers for sporadic Parkinson disease

T. K. Christopoulos, P. Ioannou, Stella Kouloulia, 2017, Microchimica Acta.

Binding studies using ion-selective electrodes. Examination of the picrate-albumin interaction as a model system.

E. Diamandis, T. K. Christopoulos, 1990, Analytical chemistry.

Screening non-deletion α-thalassaemia mutations in the HBA1 and HBA2 genes by high-resolution melting analysis

T. K. Christopoulos, J. Traeger-Synodinos, E. Kanavakis, 2015, Clinical chemistry and laboratory medicine.

Lateral flow dipstick test for genotyping of 15 beta-globin gene (HBB) mutations with naked-eye detection.

T. K. Christopoulos, J. Traeger-Synodinos, E. Kanavakis, 2012, Analytica chimica acta.

Quantitative polymerase chain reaction using a recombinant DNA internal standard and time-resolved fluorometry.

T. K. Christopoulos, M. Verhaegen, S. Bortolin, 1996, Analytical chemistry.

Enzymatically amplified time-resolved fluorescence immunoassay with terbium chelates.

E. Diamandis, T. K. Christopoulos, 1992, Analytical chemistry.

Expression hybridization assays combining cDNAs from firefly and Renilla luciferases as labels for simultaneous determination of two target sequences.

T. K. Christopoulos, P. Obeid, P. Ioannou, 2000, Analytical chemistry.

Posidonia oceanica (Mediterranean tapeweed) leaf litter as a source of fluorescent carbon dot preparations

Despina P. Kalogianni, T. K. Christopoulos, Natalia-Maria Christopoulou, 2021 .

High-throughput microtiter well-based chemiluminometric genotyping of 15 HBB gene mutations in a dry-reagent format.

T. K. Christopoulos, E. Kanavakis, J. Traeger-Synodinos, 2007, Clinical chemistry.

Multianalyte quantitative competitive PCR on optically encoded microspheres for an eight-gene panel related to prostate cancer

Despina P. Kalogianni, A. Scorilas, T. K. Christopoulos, 2017, Analytical and Bioanalytical Chemistry.

Affinity capture-facilitated preparation of aequorin- oligonucleotide conjugates for rapid hybridization assays.

T. K. Christopoulos, P. Ioannou, K. Glynou, 2003, Bioconjugate chemistry.

High-throughput microtiter well-based bioluminometric genotyping of two single-nucleotide polymorphisms in the toll-like receptor-4 gene.

T. K. Christopoulos, E. Kanavakis, J. Traeger-Synodinos, 2008, Analytical biochemistry.

Two-site expression immunoassay using a firefly luciferase-coding DNA label.

T. K. Christopoulos, N. Chiu, N H Chiu, 1999, Clinical chemistry.

Hybridization assays using an expressible DNA fragment encoding firefly luciferase as a label.

T. K. Christopoulos, N. Chiu, 1996, Analytical chemistry.

Expression immunoassay. Antigen quantitation using antibodies labeled with enzyme-coding DNA fragments.

T. K. Christopoulos, N. Chiu, 1995, Analytical chemistry.

T7 RNA polymerase as a self-replicating label for antigen quantification.

B. Tannous, T. K. Christopoulos, E. Laios, 2002, Nucleic acids research.

Quadruple-allele dipstick test for simultaneous visual genotyping of A896G (Asp299Gly) and C1196T (Thr399Ile) polymorphisms in the toll-like receptor-4 gene.

T. K. Christopoulos, J. Traeger-Synodinos, M. Tzetis, 2011, Clinica chimica acta; international journal of clinical chemistry.

Quadruple-allele chemiluminometric assay for simultaneous genotyping of two single-nucleotide polymorphisms.

T. K. Christopoulos, P. Ioannou, Dimitrios S Elenis, 2009, The Analyst.

Potentiometric titration of organic cations with sodium tetraphenylborate and a liquid-membrane tetraphenylborate ion-selective electrode

E. Diamandis, T. K. Christopoulos, T. Hadjiioannou, 1982 .

Dipstick test for DNA-based food authentication. Application to coffee authenticity assessment.

T. K. Christopoulos, G. Tucker, P. Kalaitzis, 2012, Journal of agricultural and food chemistry.

24 – DEVELOPMENT OF IN-HOUSE IMMUNOASSAYS

E. Diamandis, T. K. Christopoulos, M. Khosravi, 1996 .

Flow-through units for solid-state, liquid and PVC matrix membrane ion-selective electrodes to minimise streaming potentials

E. Diamandis, T. K. Christopoulos, 1987 .

Construction of ion-selective electrodes for chlorpromazine, amitriptyline, propantheline and meperidine: analytical study and application to pharmaceutical analysis.

E. Diamandis, T. K. Christopoulos, T. Hadjiioannou, 1985, The Analyst.

Fluorometric and time-resolved immunofluorometric assays for protein-tyrosine phosphatase activity.

T. K. Christopoulos, B. Galvan, 1996, Clinical biochemistry.

Ultrasensitive Time-Resolved Fluorescence Method for α-Fetoprotein

E. Diamandis, T. K. Christopoulos, E. S. Lianidou, 1990 .

Ultrasensitive thyrotropin immunoassay based on enzymatically amplified time-resolved fluorescence with a terbium chelate.

E. Diamandis, T. K. Christopoulos, A. Papanastasiou-Diamandi, 1992, Clinical chemistry.

Europium chelate labels in time-resolved fluorescence immunoassays and DNA hybridization assays.

E. Diamandis, T. K. Christopoulos, E P Diamandis, 1990, Analytical chemistry.

Polymerase Chain Reaction and Other Amplification Systems

T. K. Christopoulos, 2006 .

Microfabricated Systems for Nucleic Acid Analysis

T. K. Christopoulos, P. Obeid, 2004, Critical reviews in clinical laboratory sciences.

High-throughput chemiluminometric genotyping of single nucleotide polymorphisms of histamine, serotonin, and adrenergic receptor genes.

T. K. Christopoulos, C. Flordellis, P. Ioannou, 2009, Analytical biochemistry.

High-throughput double quantitative competitive polymerase chain reaction for determination of genetically modified organisms.

T. K. Christopoulos, P. Ioannou, T. Koraki, 2005, Analytical chemistry.

Dry reagent dipstick test combined with 23S rRNA PCR for molecular diagnosis of bacterial infection in arthroplasty.

Elias Panagiotopoulos, Despina P Kalogianni, Penelope C Ioannou, 2007, Analytical biochemistry.

Continuous-flow potentiometric determination of α-amylase activity in serum and urine

E. Diamandis, T. K. Christopoulos, T. Hadjiioannou, 1985 .

3 – THEORY OF IMMUNOASSAYS

Eleftherios P. Diamandis, Theodore K. Christopoulos, E. Diamandis, 1996 .

Τwo-panel molecular testing for genetic predisposition for thrombosis using multi-allele visual biosensors

T. K. Christopoulos, P. Ioannou, Alexandra Iliadi, 2016, Analytical and Bioanalytical Chemistry.

Paper-based device providing visual genetic signatures for precision medicine: application to breast cancer

Penelope C Ioannou, Theodore K Christopoulos, Aikaterini Galaziou, 2019, Analytical and Bioanalytical Chemistry.

Lateral flow devices for nucleic acid analysis exploiting quantum dots as reporters.

Sotirios S. Tragoulias, Theodore K. Christopoulos, Despina P. Kalogianni, 2015, Analytica chimica acta.

Carbon nano-strings as reporters in lateral flow devices for DNA sensing by hybridization

Despina P Kalogianni, Penelope C Ioannou, Theodore K Christopoulos, 2011, Analytical and bioanalytical chemistry.

Dry‐reagent disposable biosensor for visual genotyping of single nucleotide polymorphisms by oligonucleotide ligation reaction: application to pharmacogenetic analysis

T. K. Christopoulos, A. Gravanis, P. Ioannou, 2008, Human mutation.

Time-resolved fluorometric hybridization assays with RNA probes synthesized from polymerase chain reaction-generated DNA templates.

T. K. Christopoulos, P. Radovich, S. Bortolin, 1995, Analytical chemistry.

Visual screening for JAK2V617F mutation by a disposable dipstick

Jan Traeger-Synodinos, Theodore K. Christopoulos, T. K. Christopoulos, 2010, Analytical and bioanalytical chemistry.

Potentiometric titration of pharmaceutical compounds in formulations with sodium tetraphenylborate

E. Diamandis, T. K. Christopoulos, 1983 .

Ultrasensitive determination of europium using microsecond time-resolved spectrofluorimetry

E. Diamandis, T. K. Christopoulos, 1991 .

Nanoparticle-based DNA biosensor for visual detection of genetically modified organisms.

Despina P Kalogianni, Penelope C Ioannou, Theodore K Christopoulos, 2006, Biosensors & bioelectronics.

Ion-selective electrodes for the determination of ionisation constants of sparingly soluble organic bases in aqueous solutions: applications to chlorpromazine and amitriptyline

E. Diamandis, T. K. Christopoulos, A. Mitsana-Papazoglou, 1985 .

A general method for the assay of binders. Application to the potentiometric determination of albumin in human serum, plasma and whole blood.

E. Diamandis, T. K. Christopoulos, 1986, Clinical biochemistry.

Macromolecular crowding agents enhance the sensitivity of lateral flow immunoassays

Despina P. Kalogianni, T. K. Christopoulos, Natalia-Maria Christopoulou, 2022, Biosensors and Bioelectronics.

Method for rapid conjugation of recombinant photoprotein aequorin with streptavidin and application as a universal detection reagent for binding assays

T. K. Christopoulos, P. Ioannou, Panayotis G. Zerefos, 2006 .

Combined flash- and glow-type chemiluminescent reactions for high-throughput genotyping of biallelic polymorphisms.

Theodore K Christopoulos, B. Tannous, T. K. Christopoulos, 2003, Analytical biochemistry.

Bacterial expression of in vivo-biotinylated aequorin for direct application to bioluminometric hybridization assays.

T. K. Christopoulos, M. Verhaegen, 2002, Analytical biochemistry.

Bioluminescence hybridization assays using recombinant aequorin. Application to the detection of prostate-specific antigen mRNA.

T. K. Christopoulos, B. Galvan, 1996, Analytical chemistry.

Genotyping of single nucleotide polymorphisms by primer extension reaction and a dual-analyte bio/chemiluminometric assay

T. K. Christopoulos, P. Ioannou, Jessica K Konstantou, 2007, Analytical and bioanalytical chemistry.

Lateral flow test for meat authentication with visual detection.

Despina P Kalogianni, Theodore K Christopoulos, Despina P. Kalogianni, 2019, Food chemistry.

Time-Resolved Fluorescence

Eleftherios P. Diamandis, Theodore K. Christopoulos, E. Diamandis, 2000 .

Quadruple-analyte chemiluminometric hybridization assay. Application to double quantitative competitive polymerase chain reaction.

T. K. Christopoulos, P. Ioannou, Dimitrios S Elenis, 2007, Analytical chemistry.

A universal lateral flow assay for microRNA visual detection in urine samples.

Despina P. Kalogianni, T. K. Christopoulos, Panagiota M Kalligosfyri, 2023, Talanta.

Advances in microRNA analysis

Despina P. Kalogianni, T. K. Christopoulos, Panagiota M Kalligosfyri, 2017, Analytical and Bioanalytical Chemistry.

Quantitative western blot analysis and spot immunodetection using time-resolved fluorometry.

E. Diamandis, T. K. Christopoulos, C. Bean, 1992, Journal of immunological methods.

Duplex RT-PCR and chemiluminometric hybridization assay for combined screening of the mRNAs of prostate-specific antigen and prostate-specific membrane antigen in peripheral blood

B. Tannous, T. K. Christopoulos, E. Emmanouilidou, 2005 .

Advances in molecular techniques for the detection and quantification of genetically modified organisms

Despina P Kalogianni, Penelope C Ioannou, Theodore K Christopoulos, 2008, Analytical and bioanalytical chemistry.

Association of TLR4 single-nucleotide polymorphisms and sarcoidosis in Greek patients.

T. K. Christopoulos, J. Traeger-Synodinos, M. Tzetis, 2009, Genetic testing and molecular biomarkers.

Genotyping of single-nucleotide polymorphisms by primer extension reaction in a dry-reagent dipstick format.

T. K. Christopoulos, E. Kanavakis, J. Traeger-Synodinos, 2007, Analytical chemistry.

Home-built integrated microarray system (IMAS). A three-laser confocal fluorescence scanner coupled with a microarray printer

Sotirios S. Tragoulias, Pierre J. Obeid, Ioannis E. Tataridis, 2008, Analytical and bioanalytical chemistry.

Detection of prostate-specific antigen mRNA by reverse transcription polymerase chain reaction and time-resolved fluorometry.

E. Diamandis, T. K. Christopoulos, B. Galvan, 1995, Clinical chemistry.

Detection of BCR-ABL transcripts from the Philadelphia translocation by hybridization in microtiter wells and time-resolved immunofluorometry.

T. K. Christopoulos, S. Bortolin, 1995, Clinical chemistry.

Time-resolved immunofluorometric determination of specific mRNA sequences amplified by the polymerase chain reaction.

T. K. Christopoulos, S. Bortolin, 1994, Analytical chemistry.

A simplified approach for FSHD molecular testing.

T. K. Christopoulos, J. Traeger-Synodinos, E. Kanavakis, 2014, Clinica chimica acta; international journal of clinical chemistry.

A nanoparticle-based sensor for visual detection of multiple mutations

Penelope C Ioannou, Theodore K Christopoulos, T. K. Christopoulos, 2011, Nanotechnology.

Multianalyte, dipstick-type, nanoparticle-based DNA biosensor for visual genotyping of single-nucleotide polymorphisms.

Jan Traeger-Synodinos, Penelope C Ioannou, Theodore K Christopoulos, 2009, Biosensors & bioelectronics.

Digital camera and smartphone as detectors in paper-based chemiluminometric genotyping of single nucleotide polymorphisms

Elena M Spyrou, Despina P Kalogianni, Sotirios S Tragoulias, 2016, Analytical and Bioanalytical Chemistry.

Quantification of nucleic acids on nitrocellulose membranes with time-resolved fluorometry.

E. Diamandis, T. K. Christopoulos, G. Wilson, 1991, Nucleic acids research.

Sandwich-type deoxyribonucleic acid hybridization assays based on enzyme amplified time-resolved fluorometry.

T. K. Christopoulos, N. Chiu, J. Peltier, 1998, The Analyst.

Potentiometric measurements in air-segmented streams without debubbling

E. Diamandis, T. K. Christopoulos, 1989 .

Olive oil DNA fingerprinting by multiplex SNP genotyping on fluorescent microspheres.

Despina P. Kalogianni, T. K. Christopoulos, P. Kalaitzis, 2015, Journal of agricultural and food chemistry.

High-throughput microtiter well-based bioluminometric genotyping of two single-nucleotide polymorphisms in the toll-like receptor-4 gene.

T. K. Christopoulos, E. Kanavakis, J. Traeger-Synodinos, 2008, Analytical biochemistry.

Multi-allele DNA biosensor for the rapid genotyping of 'nondeletion' alpha thalassaemia mutations in HBA1 and HBA2 genes by means of multiplex primer extension reaction.

T. K. Christopoulos, J. Traeger-Synodinos, E. Kanavakis, 2015, Clinica chimica acta; international journal of clinical chemistry.