S. Klimašauskas

发表

5-hmC in the brain is abundant in synaptic genes and shows differences at the exon-intron boundary

B. Blencowe, P. Kapranov, R. Kustra, 2012, Nature Structural &Molecular Biology.

Hhal methyltransferase flips its target base out of the DNA helix

R. Roberts, Xiaodong Cheng, S. Klimašauskas, 1994, Cell.

Processive Methylation of Hemimethylated CpG Sites by Mouse Dnmt1 DNA Methyltransferase*

S. Klimašauskas, G. Vilkaitis, I. Suetake, 2005, Journal of Biological Chemistry.

M.HhaI binds tightly to substrates containing mismatches at the target base.

R. Roberts, S. Klimašauskas, 1995, Nucleic acids research.

Chemical display of thymine residues flipped out by DNA methyltransferases.

R. Roberts, S. Klimašauskas, E. Weinhold, 1998, Nucleic acids research.

Functional Roles of the Conserved Threonine 250 in the Target Recognition Domain of HhaI DNA Methyltransferase*

Xiaodong Cheng, S. Klimašauskas, E. Weinhold, 2000, The Journal of Biological Chemistry.

DNA Labeling Using DNA Methyltransferases.

Edita Kriukienė, S. Klimašauskas, M. Tomkuvienė, 2016, Advances in experimental medicine and biology.

Bisulfite sequencing protocol displays both 5-methylcytosine and N4-methylcytosine.

S. Klimašauskas, G. Vilkaitis, 1999, Analytical biochemistry.

Programmable sequence-specific click-labeling of RNA using archaeal box C/D RNP methyltransferases

Ignas Černiauskas, S. Klimašauskas, E. Weinhold, 2012, Nucleic acids research.

Photocage-Selective Capture and Light-Controlled Release of Target Proteins

S. Klimašauskas, A. Zvirbliene, Martynas Simanavičius, 2020, iScience.

Repurposing enzymatic transferase reactions for targeted labeling and analysis of DNA and RNA.

S. Klimašauskas, G. Vilkaitis, M. Tomkuvienė, 2019, Current opinion in biotechnology.

Biophysical Approaches To Study Dna Base Flipping

Z. Liutkevičiūtė, S. Klimašauskas, D. Daujotytė, 2009 .

The stereochemistry of benzo[a]pyrene‐2′‐deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases

D. Maltseva, S. Klimašauskas, N. Geacintov, 2007, The FEBS journal.

Interaction of AluI, Cfr6I and PvuII restriction-modification enzymes with substrates containing either N4-methylcytosine or 5-methylcytosine.

A. Janulaitis, S. Klimašauskas, V. Butkus, 1987, Biochimica et biophysica acta.

Chemistry of AdoMet‐dependent methyltransferases.

S. Klimašauskas, G. Lukinavičius, 2008 .

Alleviation of C⋅C Mismatches in DNA by the Escherichia coli Fpg Protein

P. Ruoff, S. Klimašauskas, A. Klungland, 2021, Frontiers in Microbiology.

HhaI DNA methyltransferase uses the protruding Gln237 for active flipping of its target cytosine.

Č. Venclovas, S. Klimašauskas, G. Vilkaitis, 2004, Structure.

Crystal structure of the HhaI DNA methyltransferase.

R. Roberts, S. Klimašauskas, S. Kumar, 1993, Cold Spring Harbor symposia on quantitative biology.

Alw26I, Eco31I and Esp3I--type IIs methyltransferases modifying cytosine and adenine in complementary strands of the target DNA.

J. Bitinaite, A. Janulaitis, S. Klimašauskas, 1992, Nucleic acids research.

Enhanced chemical stability of adomet analogues for improved methyltransferase-directed labeling of DNA.

S. Klimašauskas, G. Lukinavičius, M. Tomkuvienė, 2013, ACS chemical biology.

Direct decarboxylation of 5-carboxylcytosine by DNA C5-methyltransferases.

Edita Kriukienė, Z. Liutkevičiūtė, S. Klimašauskas, 2014, Journal of the American Chemical Society.

Interplay between the trigger loop and the F loop during RNA polymerase catalysis

S. Klimašauskas, I. Artsimovitch, V. Nikiforov, 2013, Nucleic acids research.

Allosteric control of catalysis by the F loop of RNA polymerase

S. Klimašauskas, I. Artsimovitch, V. Nikiforov, 2009, Proceedings of the National Academy of Sciences.

The sequence specificity domain of cytosine-C5 methylases.

R. Roberts, J. Nelson, S. Klimašauskas, 1991, Nucleic acids research.

Methyltransferase-directed derivatization of 5-hydroxymethylcytosine in DNA.

Edita Kriukienė, Z. Liutkevičiūtė, S. Klimašauskas, 2011, Angewandte Chemie.

Cytosine-5-methyltransferases add aldehydes to DNA.

Z. Liutkevičiūtė, S. Klimašauskas, G. Lukinavičius, 2009, Nature chemical biology.

Synthesis of S‐Adenosyl‐L‐Methionine Analogs with Extended Transferable Groups for Methyltransferase‐Directed Labeling of DNA and RNA

S. Klimašauskas, V. Masevičius, Milda Nainytė, 2016, Current protocols in nucleic acid chemistry.

Oligonucleotide-Addressed Covalent 3'-Terminal Derivatization of Small RNA Strands for Enrichment and Visualization.

S. Klimašauskas, G. Vilkaitis, A. Plotnikova, 2017, Angewandte Chemie.

Characterization of restriction‐modification enzymes Cfr13 I from Citrobacter freundii RFL13

J. Bitinaite, A. Janulaitis, S. Klimašauskas, 1985, FEBS letters.

Enzymatic Hydroxylation and Excision of Extended 5-Methylcytosine Analogues

S. Klimašauskas, M. Bochtler, M. Ravichandran, 2020, Journal of molecular biology.

Modulation of RNA polymerase activity through the trigger loop folding

S. Klimašauskas, I. Artsimovitch, V. Nikiforov, 2010, Transcription.

Usage of rRNA-methyltransferase for site-specific fluorescent labeling

P. Sergiev, O. Dontsova, S. Klimašauskas, 2012, Moscow University Chemistry Bulletin.

FANCD2 maintains replication fork stability during misincorporation of the DNA demethylation products 5-hydroxymethyl-2’-deoxycytidine and 5-hydroxymethyl-2’-deoxyuridine

S. Klimašauskas, C. García-Calderón, I. V. Rosado, 2022, Cell Death & Disease.

A new tool for biotechnology: AdoMet-dependent methyltransferases.

S. Klimašauskas, E. Weinhold, 2007, Trends in biotechnology.

Lineage-specific variations in the trigger loop modulate RNA proofreading by bacterial RNA polymerases

S. Klimašauskas, G. Belogurov, A. Kulbachinskiy, 2016, Nucleic acids research.

A basal promoter element recognized by free RNA polymerase sigma subunit determines promoter recognition by RNA polymerase holoenzyme.

K. Severinov, S. Klimašauskas, T. Heyduk, 2006, Molecular cell.

Specific Recognition of the -10 Promoter Element by the Free RNA Polymerase σ Subunit*

S. Klimašauskas, T. Heyduk, A. Feklistov, 2007, Journal of Biological Chemistry.

An Additional Basal Promoter Element Recognized by Free RNA Polymerase σ Subunit Determines Promoter Recognition by RNA Polymerase Holoenzyme

K. Severinov, S. Klimašauskas, T. Heyduk, 2006 .

Recognition of guanosine by dissimilar tRNA methyltransferases.

J. Piccirilli, Z. Liutkevičiūtė, S. Klimašauskas, 2012, RNA.

Dynamic modes of the flipped‐out cytosine during HhaI methyltransferase–DNA interactions in solution

K. Wüthrich, T. Szyperski, S. Klimašauskas, 1998, The EMBO journal.

Probing a rate-limiting step by mutational perturbation of AdoMet binding in the HhaI methyltransferase

S. Klimašauskas, E. Merkienė, 2005, Nucleic acids research.

Chemical mapping of cytosines enzymatically flipped out of the DNA helix

Z. Liutkevičiūtė, S. Klimašauskas, G. Tamulaitis, 2008, Nucleic acids research.

Analysis of products of DNA modification by methylases: a procedure for the determination of 5- and N4-methylcytosines in DNA.

A. Janulaitis, S. Klimašauskas, V. Butkus, 1985, Analytical biochemistry.

Sequence motifs characteristic of DNA[cytosine-N4]methyltransferases: similarity to adenine and cytosine-C5 DNA-methylases.

A. Janulaitis, S. Klimašauskas, A. Timinskas, 1989, Nucleic acids research.

2-Aminopurine as a fluorescent probe for DNA base flipping by methyltransferases.

S. Klimašauskas, E. Weinhold, S. Serva, 1998, Nucleic acids research.

Engineering the DNA cytosine-5 methyltransferase reaction for sequence-specific labeling of DNA

S. Klimašauskas, A. Lapinaite, G. Lukinavičius, 2012, Nucleic acids research.

Direct observation of cytosine flipping and covalent catalysis in a DNA methyltransferase

S. Klimašauskas, Rūta Gerasimaitė, E. Merkienė, 2011, Nucleic acids research.

A directed evolution design of a GCG-specific DNA hemimethylase

S. Klimašauskas, G. Vilkaitis, Rūta Gerasimaitė, 2009, Nucleic acids research.

Solubility engineering of the HhaI methyltransferase.

Thomas Szyperski, Dalia Daujotyte, Giedrius Vilkaitis, 2003, Protein engineering.

The Mechanism of DNA Cytosine-5 Methylation

S. Klimašauskas, E. Weinhold, G. Vilkaitis, 2001, The Journal of Biological Chemistry.

Enzymatic C5-cytosine methylation of DNA: mechanistic implications of new crystal structures for HhaL methyltransferase-DNA-AdoHcy complexes.

R. Roberts, Xiaodong Cheng, S. Klimašauskas, 1996, Journal of molecular biology.

Direct transfer of extended groups from synthetic cofactors by DNA methyltransferases

S. Klimašauskas, E. Weinhold, G. Lukinavičius, 2006, Nature chemical biology.

DNA unmethylome profiling by covalent capture of CpG sites

Sun-Chong Wang, Daofeng Li, Ting Wang, 2013, Nature Communications.

DNA fluorocode: A single molecule, optical map of DNA with nanometre resolution

P. Dedecker, J. Hofkens, J. Hotta, 2010 .

Targeted labeling of DNA by methyltransferase-directed transfer of activated groups (mTAG).

Saulius Klimasauskas, S. Klimašauskas, E. Weinhold, 2007, Journal of the American Chemical Society.

5-Hydroxymethylcytosine--the elusive epigenetic mark in mammalian DNA.

Edita Kriukienė, Z. Liutkevičiūtė, S. Klimašauskas, 2012, Chemical Society reviews.

Cytosine modification in DNA by BcnI methylase yields N 4‐methylcytosine

A Janulaitis, A. Janulaitis, S. Klimašauskas, 1983, FEBS letters.

N4,5-dimethylcytosine, a novel hypermodified base in DNA.

S. Klimašauskas, G. Vilkaitis, S. Kulakauskas, 2002, Nucleic acids research. Supplement.

Excision of the doubly methylated base N4,5-dimethylcytosine from DNA by Escherichia coli Nei and Fpg proteins

S. Klimašauskas, M. Bjørås, A. Klungland, 2018, Philosophical Transactions of the Royal Society B: Biological Sciences.

Approaches for studying microRNA and small interfering RNA methylation in vitro and in vivo.

Xuemei Chen, S. Klimašauskas, G. Vilkaitis, 2007, Methods in enzymology.

Kinetic and functional analysis of the small RNA methyltransferase HEN1: the catalytic domain is essential for preferential modification of duplex RNA.

S. Klimašauskas, G. Vilkaitis, A. Plotnikova, 2010, RNA.

Precise genomic mapping of 5-hydroxymethylcytosine via covalent tether-directed sequencing

Edita Kriukienė, S. Klimašauskas, Milda Narmontė, 2020, PLoS biology.

The DNA (cytosine-5) methyltransferases

Xiaodong Cheng, Sanjay Kumar, Richard J. Roberts, 1994, Nucleic Acids Res..

Tethered Oligonucleotide-Primed Sequencing, TOP-Seq: A High-Resolution Economical Approach for DNA Epigenome Profiling.

Edita Kriukienė, S. Klimašauskas, Povilas Gibas, 2017, Molecular cell.

5-Hydroxymethylcytosine — The Elusive Epigenetic Mark in Mammalian DNA

Edita Kriukienė, Z. Liutkevičiūtė, S. Klimašauskas, 2013 .

Selective chemical tracking of Dnmt1 catalytic activity in live cells

S. Klimašauskas, G. Vilkaitis, V. Masevičius, 2022, Molecular cell.

Functional mapping of the plant small RNA methyltransferase: HEN1 physically interacts with HYL1 and DICER-LIKE 1 proteins

Č. Venclovas, S. Klimašauskas, G. Vilkaitis, 2015, Nucleic acids research.

Selective covalent labeling of miRNA and siRNA duplexes using HEN1 methyltransferase.

S. Klimašauskas, G. Vilkaitis, A. Plotnikova, 2014, Journal of the American Chemical Society.

Mechanistic insights into small RNA recognition and modification by the HEN1 methyltransferase.

S. Klimašauskas, G. Vilkaitis, A. Plotnikova, 2013, The Biochemical journal.

Mechanistic insights into small RNA recognition and modification by the HEN 1 methyltransferase

S. Klimašauskas, G. Vilkaitis, A. Plotnikova, 2013 .

An Adversarial DNA N6-Methyladenine-Sensor Network Preserves Polycomb Silencing

S. Klimašauskas, S. Kweon, D. Feldman, 2019, Molecular cell.

Purification and properties of the Eco57I restriction endonuclease and methylase--prototypes of a new class (type IV).

A. Janulaitis, S. Klimašauskas, V. Butkus, 1992, Nucleic acids research.

Enhanced nucleosome assembly at CpG sites containing an extended 5-methylcytosine analogue

S. Klimašauskas, V. Kairys, Markus Meier, 2022, Nucleic acids research.

Archaeal fibrillarin–Nop5 heterodimer 2′-O-methylates RNA independently of the C/D guide RNP particle

Z. Liutkevičiūtė, S. Klimašauskas, I. Olendraite, 2017, RNA.

Restriction endonucleases of a new type.

J. Bitinaite, A. Janulaitis, S. Klimašauskas, 1988, Gene.

Animal Hen1 2′-O-methyltransferases as tools for 3′-terminal functionalization and labelling of single-stranded RNAs

S. Klimašauskas, G. Vilkaitis, A. Plotnikova, 2018, Nucleic acids research.

Distribution and regulatory roles of oxidized 5-methylcytosines in DNA and RNA of the basidiomycete fungi Laccaria bicolor and Coprinopsis cinerea

Edita Kriukienė, S. Klimašauskas, G. Petraitytė, 2022, Open Biology.

Cleavage of methylated CCCGGG sequences containing either N4-methylcytosine or 5-methylcytosine with MspI, HpaII, SmaI, XmaI and Cfr9I restriction endonucleases.

A. Janulaitis, S. Klimašauskas, V. Butkus, 1987, Nucleic acids research.

Investigation of restriction-modification enzymes from M. varians RFL19 with a new type of specificity toward modification of substrate.

A. Janulaitis, S. Klimašauskas, D. Kersulyte, 1985, Nucleic acids research.

Disruption of the target G-C base-pair by the HhaI methyltransferase.

R. Roberts, S. Klimašauskas, 1995, Gene.

Cloning and sequence analysis of the genes coding for Eco57I type IV restriction-modification enzymes.

A. Janulaitis, S. Klimašauskas, A. Timinskas, 1992, Nucleic acids research.

Synthesis of S-adenosyl-L-methionine analogs and their use for sequence-specific transalkylation of DNA by methyltransferases

S. Klimašauskas, E. Weinhold, G. Lukinavičius, 2006, Nature Protocols.

Time-resolved fluorescence of 2-aminopurine as a probe of base flipping in M.HhaI–DNA complexes

Anita C Jones, David T. F. Dryden, Steven W. Magennis, 2005, Nucleic acids research.

Tandem virtual screening targeting the SRA domain of UHRF1 identifies a novel chemical tool modulating DNA methylation.

Z. Liutkevičiūtė, S. Klimašauskas, C. Bronner, 2016, European journal of medicinal chemistry.

Methyltransferase-directed orthogonal tagging and sequencing of miRNAs and bacterial small RNAs

S. Klimašauskas, G. Vilkaitis, A. Osipenko, 2021, BMC biology.

M.Smal is an N4-methylcytosine specific DNA-methylase.

A. Janulaitis, S. Klimašauskas, V. Butkus, 1990, Nucleic acids research.

Synthesis and physical characterization of DNA fragments containing N4-methylcytosine and 5-methylcytosine.

A. Janulaitis, S. Klimašauskas, L. Minchenkova, 1987, Nucleic acids research.

A. Janulaitis, S. Klimašauskas, A. Timinskas, 1995, Gene.

Circular permutation of DNA cytosine-N4 methyltransferases: in vivo coexistence in the BcnI system and in vitro probing by hybrid formation.

A. Janulaitis, S. Klimašauskas, G. Vilkaitis, 2002, Nucleic acids research.

Synthesis of S‐Adenosyl‐L‐Methionine Analogs with Extended Transferable Groups for Methyltransferase‐Directed Labeling of DNA and RNA

S. Klimašauskas, V. Masevičius, Martynas Malikenas, 2023, Current protocols.

An Adversarial DNA N6-Methyladenine-Sensor Network Preserves Polycomb Silencing

S. Klimašauskas, S. Kweon, D. Feldman, 2019, Molecular cell.

Biosynthetic selenoproteins with genetically-encoded photocaged selenocysteines.

Z. Liutkevičiūtė, S. Klimašauskas, R. Rakauskaitė, 2015, Chemical communications.

5-Hydroxymethylcytosine: the many faces of the sixth base of mammalian DNA.

Edita Kriukienė, S. Klimašauskas, M. Tomkuvienė, 2024, Chemical Society reviews.

One-pot trimodal mapping of unmethylated, hydroxymethylated, and open chromatin sites unveils distinctive 5hmC roles at dynamic chromatin loci

Edita Kriukienė, S. Klimašauskas, Milda Narmontė, 2023, Cell chemical biology.