S. Akashi

发表

Dynamic allostery in the ring protein TRAP.

J. Tame, Sam-Yong Park, J. Heddle, 2007, Journal of molecular biology.

The nature of the TRAP–Anti-TRAP complex

J. Tame, Sam-Yong Park, Masahiro Watanabe, 2009, Proceedings of the National Academy of Sciences.

Structural plasticity of a designer protein sheds light on β‐propeller protein evolution

J. Tame, S. Akashi, A. Voet, 2020, The FEBS journal.

Thermal unfolding of proteins probed by laser spray mass spectrometry.

K. Hiraoka, S. Akashi, Y. Nishimura, 2008, Rapid communications in mass spectrometry : RCM.

Comparative study of H3O+ (aq) and NH4+ (aq) on electrophoresis, protonating ability, and sodiation of proteins

K. Hiraoka, S. Akashi, S. Ninomiya, 2021, International Journal of Mass Spectrometry.

Single-Cell Native Mass Spectrometry of Human Erythrocytes.

S. Akashi, T. Konuma, W. Sakamoto, 2021, Analytical chemistry.

Screening of protein-ligand interactions under crude conditions by native mass spectrometry

T. Ikegami, S. Akashi, I. Hamachi, 2020, Analytical and Bioanalytical Chemistry.

Structural characterization of human general transcription factor TFIIF in solution

S. Akashi, Y. Nishimura, Seiji Yamamoto, 2008, Protein science : a publication of the Protein Society.

Observation of hydrogen-deuterium exchange of ubiquitin by direct analysis of electrospray capillary-skimmer dissociation with Fourier transform ion cyclotron resonance mass spectrometry.

S. Akashi, K. Takio, Y. Naito, 1999, Analytical chemistry.

Squid nerve sphingomyelin containing an unusual sphingoid base.

T. Tanaka, S. Akashi, Y. Kishimoto, 2000, Journal of lipid research.

Crystal structures of penicillin-binding protein 3 (PBP3) from methicillin-resistant Staphylococcus aureus in the apo and cefotaxime-bound forms.

E. Obayashi, H. Yoshida, F. Kawai, 2012, Journal of molecular biology.

Structural studies of the Maillard reaction products of a protein using ion trap mass spectrometry.

S. Akashi, T. Mizukoshi, K. Hirayama, 2000, Journal of mass spectrometry : JMS.

Field desorption tandem mass spectrometry of anthracycline antibiotics, cosmomycin A, B, A', B', C and D.

H. Shibai, S. Akashi, K. Hirayama, 1987, Biomedical & environmental mass spectrometry.

Quantitative Analysis of Binding Affinities of Biological Macromolecular Complexes by Laser Spray Mass Spectrometry

K. Hiraoka, S. Akashi, Y. Nishimura, 2008 .

Investigation of molecular size of transcription factor TFIIE in solution

S. Akashi, Y. Nishimura, S. Unzai, 2005, Proteins.

Primary structure of Paim I, an alpha-amylase inhibitor from Streptomyces corchorushii, determined by the combination of Edman degradation and fast atom bombardment mass spectrometry.

K. Tanaka, S. Akashi, S. Murao, 1987, Biochemistry.

Crystal structures of the clock protein EA4 from the silkworm Bombyx mori.

N. Shibayama, Sam-Yong Park, S. Akashi, 2008, Journal of molecular biology.

Application of the NZ‐1 Fab as a crystallization chaperone for PA tag‐inserted target proteins

S. Akashi, T. Nogi, Y. Kato, 2019, Protein science : a publication of the Protein Society.

The crystal structure and oligomeric form of Escherichia colil,d-carboxypeptidase A.

J. Tame, S. Akashi, D. Roper, 2018, Biochemical and biophysical research communications.

The crystal structure and oligomeric form of Escherichia colil,d-carboxypeptidase A.

J. Tame, S. Akashi, D. Roper, 2018, Biochemical and biophysical research communications.

Determination of the primary structure of Paim II, an alpha-amylase inhibitor from Streptomyces coruchorushii, by high-performance tandem mass spectrometry.

S. Akashi, S. Murao, K. Hirayama, 1989, Biochemical and biophysical research communications.

Characterization of the interface structure of enzyme‐inhibitor complex by using hydrogen‐deuterium exchange and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

S. Akashi, K. Takio, 2000, Protein science : a publication of the Protein Society.

Purification, crystallization and initial X-ray diffraction study of human REV7 in complex with a REV3 fragment

Hashimoto, S. Akashi, Toshiyuki Shimizu, 2009 .

Structural and biochemical analyses of the human PAD4 variant encoded by a functional haplotype gene.

H. Kurumizaka, A. Osakabe, S. Akashi, 2011, Acta crystallographica. Section D, Biological crystallography.

Metal distribution in Cu/Zn-superoxide dismutase revealed by native mass spectrometry.

M. Tajiri, S. Akashi, Y. Furukawa, 2022, Free radical biology & medicine.

A mass spectrometric approach to the study of DNA-binding proteins: interaction of human TRF2 with telomeric DNA.

M. Gross, S. Akashi, Y. Nishimura, 2008, Biochemistry.

Characterization of Stress-Exposed Granulocyte Colony Stimulating Factor Using ELISA and Hydrogen/Deuterium Exchange Mass Spectrometry

S. Akashi, Katsuyoshi Yamazaki, Daisuke Tsuchida, 2014, Journal of The American Society for Mass Spectrometry.

Evaluation of binding affinity of protein-mutant dna complexes in solution by laser spray mass spectrometry

K. Hiraoka, S. Akashi, Y. Nishimura, 2006, Journal of the American Society for Mass Spectrometry.

Hybrid in vitro/in silico analysis of low‐affinity protein–protein interactions that regulate signal transduction by Sema6D

T. Yamane, M. Ikeguchi, S. Akashi, 2022, Protein science : a publication of the Protein Society.

Structural analysis and taste evaluation of γ-glutamyl peptides comprising sulfur-containing amino acids

S. Akashi, Y. Ishiwatari, H. Wakabayashi, 2018, Bioscience, biotechnology, and biochemistry.

Function of homo- and hetero-oligomers of human nucleoplasmin/nucleophosmin family proteins NPM1, NPM2 and NPM3 during sperm chromatin remodeling

K. Nagata, S. Akashi, M. Okuwaki, 2012, Nucleic acids research.

Denaturation of α‐lactalbumin and ubiquitin studied by electrospray and laser spray

K. Hiraoka, S. Akashi, Atsushi Takamizawa, 2007 .

Stability analysis for double-stranded DNA oligomers and their noncovalent complexes with drugs by laser spray.

K. Hiraoka, S. Akashi, Y. Nishimura, 2006, Journal of mass spectrometry : JMS.

Impact of limited oxidation on protein ion mobility and structure of importance to footprinting by radical probe mass spectrometry.

S. Akashi, K. Downard, S. Maleknia, 2012, Rapid communications in mass spectrometry : RCM.

Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinant protein expression system

S. Akashi, Hideo Takahashi, M. Sakakura, 2018, Journal of Biomolecular NMR.

Structural Characterization of “Tailed Picket-Fence Porphyrins” by High-Energy Fast Atom Bombardment Collision-Induced Dissociation Mass Spectrometry/Mass Spectrometry

S. Akashi, N. Dohmae, H. Nishide, 1997 .

Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and Frit-FAB LC/MS.

S. Akashi, K. Hirayama, M. Furuya, 1990, Biochemical and biophysical research communications.

Total synthesis of (+)-tryptoquivaline

S. Akashi, Y. Hasegawa, Manabu Ito, 1985 .

Annonaceous acetogenins from the seeds of Annona squamosa. Adjacent bis-tetrahydrofuranic acetogenins

S. Akashi, T. Eguchi, K. Kakinuma, 1994 .

Structural studies of polyhydroxybis(tetrahydrofuran)acetogenins from Annona squamosa using the combination of chemical derivatization and precursor-ion scanning mass spectrometry

S. Akashi, K. Hirayama, Y. Fujimoto, 1993 .

Fission Yeast Swi5-Sfr1 Protein Complex, an Activator of Rad51 Recombinase, Forms an Extremely Elongated Dogleg-shaped Structure

M. Ikeguchi, S. Akashi, Toshiyuki Shimizu, 2011, The Journal of Biological Chemistry.

3D structural analysis of protein O‐mannosyl kinase, POMK, a causative gene product of dystroglycanopathy

S. Akashi, S. Mishra, Koichi Kato, 2017, Genes to cells : devoted to molecular & cellular mechanisms.

Mechanistic insights into intramembrane proteolysis by E. coli site-2 protease homolog RseP

O. Nureki, M. Tajiri, K. Hirata, 2022, bioRxiv.

Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinant protein expression system

S. Akashi, Hideo Takahashi, M. Sakakura, 2018, Journal of Biomolecular NMR.

Structural visualization of key steps in nucleosome reorganization by human FACT

K. Morikawa, S. Akashi, N. Miyazaki, 2019, Scientific Reports.

Structure of the N-terminal Regulatory Domain of a Plant NADPH Oxidase and Its Functional Implications*

K. Shimamoto, S. Akashi, Toshiyuki Shimizu, 2009, The Journal of Biological Chemistry.

Mass Spectrometric Characterization of Histone H3 Isolated from in-Vitro Reconstituted and Acetylated Nucleosome Core Particle

S. Akashi, S. Izumi, K. Saikusa, 2020, Mass spectrometry.

Cryo-electron microscopy structure of the H3-H4 octasome without histones H2A and H2B

H. Kurumizaka, S. Akashi, Y. Takizawa, 2021 .

Crystal Structure of Human REV7 in Complex with a Human REV3 Fragment and Structural Implication of the Interaction between DNA Polymerase ζ and REV1*

Y. Murakumo, S. Akashi, Toshiyuki Shimizu, 2010, The Journal of Biological Chemistry.

C‐terminal acidic domain of histone chaperone human NAP1 is an efficient binding assistant for histone H2A‐H2B, but not H3‐H4

K. Nagata, H. Kurumizaka, A. Osakabe, 2016, Genes to cells : devoted to molecular & cellular mechanisms.

Charge‐neutralization effect of the tail regions on the histone H2A/H2B dimer structure

H. Kurumizaka, S. Akashi, Y. Nishimura, 2015, Protein science : a publication of the Protein Society.

Native mass spectrometry of protein and DNA complexes prepared in non-volatile buffers.

H. Kurumizaka, S. Akashi, K. Saikusa, 2020, Journal of the American Society for Mass Spectrometry.

Rapid and Definitive Analysis of In Vitro DNA Methylation by Nano-electrospray Ionization Mass Spectrometry

S. Akashi, Hiroshi Ushijima, Eri Igarashi, 2019, Journal of The American Society for Mass Spectrometry.

Conclusive evidence of the reconstituted hexasome proven by native mass spectrometry.

H. Kurumizaka, S. Akashi, Y. Nishimura, 2013, Biochemistry.

Characterisation of an intrinsically disordered protein complex of Swi5-Sfr1 by ion mobility mass spectrometry and small-angle X-ray scattering.

Mamoru Sato, Mitsunori Ikeguchi, Naoyuki Kuwabara, 2013, The Analyst.

Structural characterization of mouse monoclonal antibody 13-1 against a porphyrin derivative: identification of a disulfide bond in CDR-H3 of Mab 13-1.

H. Yamaguchi, T. Torizawa, S. Akashi, 1997, Biochemical and biophysical research communications.

Characterization of mouse switch variant antibodies by matrix-assisted laser desorption ionization mass spectrometry and electrospray ionization mass spectrometry

S. Akashi, I. Shimada, Y. Arata, 1996, Journal of the American Society for Mass Spectrometry.

A determination of the positions of disulphide bonds in Paim I, alpha-amylase inhibitor from Streptomyces corchorushii, using fast atom bombardment mass spectrometry.

K. Tanaka, S. Akashi, S. Murao, 1988, Biomedical & environmental mass spectrometry.

Comprehensive Characterization of Relationship Between Higher-Order Structure and FcRn Binding Affinity of Stress-Exposed Monoclonal Antibodies

S. Akashi, Katsuyoshi Yamazaki, Daisuke Tsuchida, 2015, Pharmaceutical Research.

Characterization of the structural difference between active and inactive forms of the Ras protein by chemical modification followed by mass spectrometric peptide mapping.

S. Yokoyama, T. Terada, M. Shirouzu, 1997, Analytical biochemistry.

Top-down analysis of basic proteins by microchip capillary electrophoresis mass spectrometry.

S. Akashi, E. Suzuki, Y. Nishimura, 2006, Rapid communications in mass spectrometry : RCM.

Crystal structure of the overlapping dinucleosome composed of hexasome and octasome

H. Kono, Sam-Yong Park, Y. Ohkawa, 2017, Science.

Structure of melittin bound to phospholipid micelles studied using hydrogen-deuterium exchange and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

S. Akashi, K. Takio, 2001, Journal of the American Society for Mass Spectrometry.

Cryo–electron microscopy structure of the H3-H4 octasome: A nucleosome-like particle without histones H2A and H2B

H. Kurumizaka, S. Akashi, Y. Takizawa, 2022, Proceedings of the National Academy of Sciences of the United States of America.

Evaluation of protein-DNA binding affinity by electrospray ionization mass spectrometry

S. Akashi, Y. Nishimura, R. Osawa, 2005, Journal of the American Society for Mass Spectrometry.

[Structural and functional characterization of biological macromolecules by mass spectrometry].

S. Akashi, 2006, Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan.

Melittin-Diacylphosphatidylcholine Interaction Examined by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

S. Akashi, K. Takio, 2002 .

The primary structure of human EGF produced by genetic engineering, studied by high-performance tandem mass spectrometry.

S. Akashi, K. Hirayama, M. Furuya, 1989, Biochemical and biophysical research communications.

The Crystal Structure of the Active Domain of Anopheles Anti-platelet Protein, a Powerful Anti-coagulant, in Complex with an Antibody*

J. Tame, Sam-Yong Park, S. Akashi, 2014, The Journal of Biological Chemistry.

Deimination stabilizes histone H2A/H2B dimers as revealed by electrospray ionization mass spectrometry.

H. Kurumizaka, S. Akashi, Y. Nishimura, 2010, Journal of mass spectrometry : JMS.

Guidelines for reporting the use of capillary electrophoresis in proteomics

Chris F. Taylor, Coral Barbas, Erdmann Rapp, 2010, Nature Biotechnology.

Effect of charge on the conformation of highly basic peptides including the tail regions of histone proteins by ion mobility mass spectrometry

S. Akashi, K. Downard, 2016, Analytical and Bioanalytical Chemistry.

Structural visualization of key steps in nucleosome reorganization by human FACT

K. Morikawa, S. Akashi, N. Miyazaki, 2019, Scientific Reports.

Photoinduced in Vivo Magnetic Resonance Imaging (MRI) with Rapid CO Release from an MnCO-Protein Needle Composite.

M. Matsuo, S. Akashi, T. Ueno, 2018, Chemistry.

Solvent accessibility of the thrombin-thrombomodulin interface.

J. Mandell, E. Komives, S. Akashi, 2001, Journal of molecular biology.

Crystal structure of human REV 7 in complex with a human REV 3 fragment and structural implication of the interaction between DNA polymerase ζ and REV 1

Y. Murakumo, S. Akashi, Toshiyuki Shimizu, 2010 .

Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin.

T. Umehara, R. Roeder, K. Tsumoto, 2023, Molecular cell.

Comprehensive Characterization of Relationship Between Higher-Order Structure and FcRn Binding Affinity of Stress-Exposed Monoclonal Antibodies

S. Akashi, Katsuyoshi Yamazaki, Daisuke Tsuchida, 2016, Pharmaceutical Research.

Evaluation of Drug Responses to Human β2AR Using Native Mass Spectrometry

M. Tajiri, S. Akashi, I. Shimada, 2023, ACS omega.

Mass spectrometric approach for characterizing the disordered tail regions of the histone H2A/H2B dimer.

S. Fuchigami, H. Kurumizaka, S. Akashi, 2015, Analytical chemistry.

Structural basis of CXC chemokine receptor 1 ligand binding and activation

M. Tajiri, J. Tame, Sam-Yong Park, 2023, Nature communications.

Homology‐modelled structure of the βB2B3‐crystallin heterodimer studied by ion mobility and radical probe MS

M. Ikeguchi, S. Akashi, Yuichi Kokabu, 2011, The FEBS journal.

Stability of the βB2B3 crystallin heterodimer to increased oxidation by radical probe and ion mobility mass spectrometry.

S. Akashi, K. Downard, S. Maleknia, 2015, Journal of structural biology.

Site-specific sodiation of peptides studied by pulsed nanoelectrospray ionization

K. Hiraoka, S. Akashi, S. Ninomiya, 2023, International Journal of Mass Spectrometry.

Thermal unfolding of proteins probed by laser spray mass spectrometry.

K. Hiraoka, S. Akashi, Y. Nishimura, 2008, Rapid communications in mass spectrometry : RCM.

Investigation of molecular interaction within biological macromolecular complexes by mass spectrometry

S. Akashi, 2006, Medicinal research reviews.

Gas-phase structure of the histone multimers characterized by ion mobility mass spectrometry and molecular dynamics simulation.

S. Fuchigami, M. Ikeguchi, H. Kurumizaka, 2013, Analytical chemistry.

Analysis of the homodimeric structure of a D‐Ala‐D‐Ala metallopeptidase, VanX, from vancomycin‐resistant bacteria

S. Akashi, T. Takai, A. Nagadoi, 2024, Protein science : a publication of the Protein Society.

Structure and assembly mechanisms of toxic human islet amyloid polypeptide oligomers associated with copper† †Electronic supplementary information (ESI) available: Experimental section, Fig. S1–S18 and Tables S1 and S2. See DOI: 10.1039/c6sc00153j

S. Akashi, D. Mun, Tae Su Choi, 2016, Chemical science.