M. Cygler

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Preparation and characterization of bacterial protein complexes for structural analysis.

A. Berghuis, Z. Jia, A. Matte, 2009, Advances in protein chemistry and structural biology.

Structures of Shikimate Dehydrogenase AroE and Its Paralog YdiB

Allan Matte, Miroslaw Cygler, Adrian J. Lapthorn, 2003, Journal of Biological Chemistry.

Insights into the biology of Escherichia coli through structural proteomics

Z. Jia, A. Matte, M. Cygler, 2007, Journal of Structural and Functional Genomics.

Ser-His-Glu triad forms the catalytic site of the lipase from Geotrichum candidum

J. Schrag, M. Cygler, Yunge Li, 1991, Nature.

Relationship between sequence conservation and three‐dimensional structure in a large family of esterases, lipases, and related proteins

J. Sussman, I. Silman, M. Harel, 1993, Protein science : a publication of the Protein Society.

Catalytic Mechanism of Heparinase II Investigated by Site-directed Mutagenesis and the Crystal Structure with Its Substrate*

Robert J Linhardt, Traian Sulea, Miroslaw Cygler, 2010, The Journal of Biological Chemistry.

Antibodies to DNA

M. Cygler, W. Anderson, Jeremy S. Lee, 1988, BioEssays : news and reviews in molecular, cellular and developmental biology.

Structure of human procathepsin L reveals the molecular basis of inhibition by the prosegment.

R. Ménard, R. Coulombe, M. Cygler, 1996, The EMBO journal.

The α/β hydrolase fold

Joel L. Sussman, Miroslaw Cygler, Israel Silman, 1992 .

Recognition of carbohydrates by antibodies.

M. Cygler, 1994, Research in immunology.

The open conformation of a Pseudomonas lipase.

Y Li, D Schomburg, D. Schomburg, 1997, Structure.

A Structural Basis for the Chiral Preferences of Lipases

J. Schrag, M. Cygler, R. Kazlauskas, 1994 .

High-Level Production of RecombinantGeotrichum candidumLipases in YeastPichia pastoris

D. Tessier, M. Cygler, M. Holmquist, 1997 .

Polymorphism in the lipase genes of Geotrichum candidum strains.

T. Vernet, D. Y. Thomas, J. Schrag, 1994, European journal of biochemistry.

Analogs of reaction intermediates identify a unique substrate binding site in Candida rugosa lipase.

J. Schrag, M. Cygler, R. Kazlauskas, 1995, Biochemistry.

Quantitative continuous assay for hyaluronan synthase.

S. Withers, R. Linhardt, M. Cygler, 2007, Analytical biochemistry.

Crystal structure of Escherichia coli crotonobetainyl-CoA: carnitine CoA-transferase (CaiB) and its complexes with CoA and carnitinyl-CoA.

A. Matte, M. Cygler, E. Rangarajan, 2005, Biochemistry.

Characterization of chondroitin sulfate lyase ABC from Bacteroides thetaiotaomicron WAL2926.

M. Cygler, Yeong Shik Kim, Joon-Soo Sim, 2008, Biochemistry.

Coiled-coil helix rotation selects repressing or activating state of transcriptional regulator DhaR.

R. Shi, I. Ekiel, M. Cygler, 2014, Structure.

Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex

M. Cygler, Y. Abu Kwaik, Snake C. Jones, 2019, mBio.

Structural Mimicry by a Bacterial F Box Effector Hijacks the Host Ubiquitin-Proteasome System.

G. Kozlov, K. Gehring, M. Cygler, 2017, Structure.

Structural and Functional Studies of the Escherichia coli Phenylacetyl-CoA Monooxygenase Complex*

R. Ménard, M. Cygler, Linhua Zhang, 2011, The Journal of Biological Chemistry.

Identification of residues essential for differential fatty acyl specificity of Geotrichum candidum lipases I and II.

D. Tessier, M. Cygler, M. Holmquist, 1997, Biochemistry.

Structure and conformation of the potent antiherpes agent 9-(2-hydroxyethoxymethyl) guanine (acycloguanosine).

M. Cygler, D. Shugar, J. Kuśmierek, 1981, Biochemical and biophysical research communications.

Crystal structure of the RluD pseudouridine synthase catalytic module, an enzyme that modifies 23S rRNA and is essential for normal cell growth of Escherichia coli.

A. Matte, M. Cygler, J. Sivaraman, 2004, Journal of molecular biology.

Structure-Function Analysis of Escherichia coli MnmG (GidA), a Highly Conserved tRNA-Modifying Enzyme

A. Velázquez‐Campoy, A. Matte, R. Shi, 2009, Journal of bacteriology.

Preliminary crystal structure analysis of an Fab specific for a Salmonella O-polysaccharide antigen.

M. Cygler, M. Przybylska, D. Rose, 1990, Journal of molecular biology.

Design of noncovalent inhibitors of human cathepsin L. From the 96-residue proregion to optimized tripeptides.

E. Purisima, R. Ménard, Y. Konishi, 2002, Journal of medicinal chemistry.

Crystal Structure of StaL, a Glycopeptide Antibiotic Sulfotransferase from Streptomyces toyocaensis*

T. Sulea, A. Matte, R. Shi, 2007, Journal of Biological Chemistry.

Biochemical and Crystallographic Studies Reveal a Specific Interaction Between TRAPP Subunits Trs33p and Bet3p

B. Oh, Min-Sung Kim, M. Whiteway, 2005, Traffic.

Crystal structure of a dodecameric FMN‐dependent UbiX‐like decarboxylase (Pad1) from Escherichia coli O157: H7

L. Hung, A. Tocilj, A. Matte, 2004, Protein science : a publication of the Protein Society.

Quaternary structure of WzzB and WzzE polysaccharide copolymerases

M. Cygler, I. Rouiller, M. Bostina, 2015, Protein science : a publication of the Protein Society.

Structure of hydrogenase maturation protein HypF with reaction intermediates shows two active sites.

R. Shi, M. Cygler, R. Sawers, 2011, Structure.

Specificity of Campylobacter jejuni adhesin PEB3 for phosphates and structural differences among its ligand complexes.

A. Matte, M. Vedadi, M. Cygler, 2009, Biochemistry.

Structure of [NiFe] Hydrogenase Maturation Protein HypE from Escherichia coli and Its Interaction with HypF

A. Matte, J. Baardsnes, M. Cygler, 2007, Journal of bacteriology.

Structural context for protein N‐glycosylation in bacteria: The structure of PEB3, an adhesin from Campylobacter jejuni

A. Matte, M. Cygler, D. Watson, 2007, Protein science : a publication of the Protein Society.

Structural Mimicry by a Ba cterial F Box Effector Hijacks the Host Ubiquitin-Proteasome System Highlights

G. Kozlov, K. Gehring, M. Cygler, 2022 .

The structure of Legionella effector protein LpnE provides insights into its interaction with Oculocerebrorenal syndrome of Lowe (OCRL) protein

M. Cygler, Lei Li, I. Chung, 2019, The FEBS journal.

Crystallization and preliminary X-ray analysis of heparinase II from Pedobacter heparinus.

M. Cygler, Yunge Li, D. Shaya, 2004, Acta crystallographica. Section D, Biological crystallography.

Structural and Functional Characterization of PseC, an Aminotransferase Involved in the Biosynthesis of Pseudaminic Acid, an Essential Flagellar Modification in Helicobacter pylori*

J. Brisson, A. Matte, J. Julien, 2006, Journal of Biological Chemistry.

Uronic polysaccharide degrading enzymes.

M. Cygler, M. Garron, 2014, Current opinion in structural biology.

Liquid chromatography-mass spectrometry to study chondroitin lyase action pattern.

Zhenqing Zhang, R. Linhardt, M. Cygler, 2009, Analytical biochemistry.

Design of a linker for trivalent thrombin inhibitors: interaction of the main chain of the linker with thrombin.

E. Purisima, Y. Konishi, M. Cygler, 1993, Biochemistry.

Snapshots along the pathway

M. Cygler, 1993, Nature.

Roles of individual EF-hands in the activation of m-calpain by calcium.

M. Cygler, P. Grochulski, P. Dutt, 2000, The Biochemical journal.

Structural and Functional Characterization of Legionella pneumophila Effector MavL

M. Cygler, E. Hartland, I. Chung, 2021, Biomolecules.

RAT PROCATHEPSIN B

R. Coulombe, M. Cygler, P. Grochulski, 1997 .

The Contribution of Structural Proteomics to Understanding the Function of Hypothetical Proteins

Z. Jia, A. Matte, M. Cygler, 2008 .

Structurally unique interaction of RBD-like and PH domains is crucial for yeast pheromone signaling

T. Sulea, M. Whiteway, J. Baardsnes, 2013, Molecular biology of the cell.

Proregion structure of members of the papain superfamily. Mode of inhibition of enzymatic activity.

M. Cygler, J. Mort, 1997, Biochimie.

Structural and Functional Investigations of the Effector Protein LpiR1 from Legionella pneumophila*

Deborah H Anderson, M. Cygler, Deborah H. Anderson, 2016, The Journal of Biological Chemistry.

Crystal Structure of N-Succinylarginine Dihydrolase AstB, Bound to Substrate and Product, an Enzyme from the Arginine Catabolic Pathway of Escherichia coli*

A. Tocilj, A. Matte, J. Schrag, 2005, Journal of Biological Chemistry.

Binding the atypical RA domain of Ste50p to the unfolded Opy2p cytoplasmic tail is essential for the high-osmolarity glycerol pathway.

T. Sulea, M. Whiteway, I. Ekiel, 2009, Molecular biology of the cell.

A full-symmetry translation function based on electron density.

M. Cygler, M. Desrochers, 1989, Acta crystallographica. Section A, Foundations of crystallography.

Crystallization and preliminary X-ray analysis of PaaAC, the main component of the hydroxylase of the Escherichia coli phenylacetyl-coenzyme A oxygenase complex.

M. Cygler, Linhua Zhang, A. Grishin, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation.

J. Schwartz, M. Cygler, L. Masson, 1995, Journal of molecular biology.

Targeting of microvillus protein Eps8 by the NleH effector kinases from enteropathogenic E. coli

Nichollas E. Scott, Isabella M. Gaeta, M. Cygler, 2022, Proceedings of the National Academy of Sciences of the United States of America.

Structure-Guided Investigation of Lipopolysaccharide O-Antigen Chain Length Regulators Reveals Regions Critical for Modal Length Control

M. Cygler, M. Valvano, S. Kalynych, 2011, Journal of bacteriology.

Design of potent bivalent thrombin inhibitors based on hirudin sequence: incorporation of nonsubstrate-type active site inhibitors.

Y. Konishi, M. Cygler, B. Gibbs, 1994, Biochemistry.

Salmonella Disrupts Host Endocytic Trafficking by SopD2-Mediated Inhibition of Rab7.

S. Grinstein, R. Shi, M. Cygler, 2015, Cell reports.

Issue information

David W. Taylor, E. Marcotte, Y. Ashani, 2021 .

Crystallization and preliminary X-ray analysis of chondroitinase B from Flavobacterium heparinum.

A. Matte, Y. Li, M. Cygler, 1999, Acta crystallographica. Section D, Biological crystallography.

Crystallization and preliminary analysis of chondroitinase AC from Flavobacterium heparinum.

Y. Li, M. Cygler, M. Allaire, 1998, Acta crystallographica. Section D, Biological crystallography.

Crystallization of the bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase domain of the human trifunctional enzyme

J. Pelletier, R. Mackenzie, Y. Li, 1996, Proteins.

N-terminal tyrosine of ISCU2 triggers [2Fe-2S] cluster synthesis by ISCU2 dimerization

D. Winge, M. Cygler, R. Lill, 2021, Nature Communications.

Handbook of metalloproteins

R. Huber, K. Wieghardt, W. Bode, 2006 .

Sequence Alignment of Esterases and Lipases Based on 3-D Structures of Two Members of This Family

J. Sussman, I. Silman, M. Harel, 1992 .

Crystal structure of wild‐type human procathepsin K

R. Ménard, M. Cygler, J. Sivaraman, 1999, Protein science : a publication of the Protein Society.

Preliminary studies on the crystallization of beef heart cytochrome c oxidase by vapor diffusion.

Y. Li, M. Cygler, P. Nicholls, 1990, Biochemical and biophysical research communications.

The KH domain facilitates the substrate specificity and unwinding processivity of DDX43 helicase.

M. Cygler, A. Kusalik, V. Vidhyasagar, 2020, The Journal of biological chemistry.

Structures of three inhibitor complexes provide insight into the reaction mechanism of the human methylenetetrahydrofolate dehydrogenase/cyclohydrolase.

R. Mackenzie, M. Cygler, J. Toth, 2000, Biochemistry.

CRYSTAL STRUCTURE OF METHANOBACTERIUM THERMAOAUTOTROPHICUM PHOSPHORIBOSYL-AMP

T. Sulea, J. Schrag, V. Davisson, 2005 .

Crystal structure of Methanobacterium thermoautotrophicum phosphoribosyl-AMP cyclohydrolase HisI.

T. Sulea, J. Schrag, M. Cygler, 2005, Biochemistry.

Active site of chondroitin AC lyase revealed by the structure of enzyme-oligosaccharide complexes and mutagenesis.

R. Linhardt, M. Cygler, W. Huang, 2001, Biochemistry.

Ca2+‐Binding domain VI of rat calpain is a homodimer in solution: Hydrodynamic, crystallization and preliminary X‐ray diffraction studies

C. Kay, M. Cygler, P. Davies, 1996, Protein science : a publication of the Protein Society.

Asparagine 405 of heparin lyase II prevents the cleavage of glycosidic linkages proximate to a 3‐O‐sulfoglucosamine residue

J. Esko, R. Linhardt, M. Cygler, 2011, FEBS Letters.

A Structural Basis for the Chiral Preferences of Lipases

Miroslaw Cygler, Joseph D. Schrag, Romas J. Kazlauskas, 1995 .

Crystal structure of chondroitinase B from Flavobacterium heparinum and its complex with a disaccharide product at 1.7 A resolution.

A. Matte, R. Linhardt, Y. Li, 1999, Journal of molecular biology.

Purification and characterization of aPenicillium sp. lipase which discriminates against diglycerides

J. Schrag, M. Cygler, E. Ziomek, 1996, Lipids.

Comparison of solid state and solution conformations of R and S epimers of 8,5'-cycloadenosine and their relevance to some enzymatic reactions.

M. Cygler, R. Stolarski, D. Shugar, 1981, Biochemistry.

Studies of azo and azoxy dyestuffs. Part 17. Synthesis and structure determination of isomeric α and β phenylazoxypyridines, N-oxides, and methiodides. A reexamination of the oxidation of phenylazopyridines and X-ray structure analyses of 4-(phenyl-α-azoxy)pyridinium methiodide and 4-(phenyl-β-azoxy

M. Cygler, K. Varughese, G. Birnbaum, 1984 .

Synthesis, structural, and biological studies on a pseudodisaccharide containing a bicyclic, bridged carba-sugar

L. Szilágyi, R. Linhardt, M. Cygler, 2011 .

Essential Roles of Zinc Ligation and Enzyme Dimerization for Catalysis in the Aminoacylase-1/M20 Family*

R. Ménard, H. Lindner, M. Cygler, 2003, Journal of Biological Chemistry.

Molecules of Escherichia coli MobB assemble into densely packed hollow cylinders in a crystal lattice with 75% solvent content.

A. Tocilj, A. Matte, M. Cygler, 2003, Acta crystallographica. Section D, Biological crystallography.

Three-dimensional structure of 2-amino-3-ketobutyrate CoA ligase from Escherichia coli complexed with a PLP-substrate intermediate: inferred reaction mechanism.

A. Matte, Y. Li, J. Schrag, 2001, Biochemistry.

Structural Basis for Fe–S Cluster Assembly and tRNA Thiolation Mediated by IscS Protein–Protein Interactions

Allan Matte, Miroslaw Cygler, A. Matte, 2010, PLoS biology.

Structure and functional dynamics of the mitochondrial Fe/S cluster synthesis complex

M. Cygler, R. Lill, S. Freibert, 2017, Nature Communications.

A Medium-Throughput Structural Proteomics Approach Applied to the Genome of E. coli

Allan Matte, Kalle Gehring, Irena Ekiel, 2009 .

Mechanism of action and NAD+-binding mode revealed by the crystal structure of l-histidinol dehydrogenase

A. Matte, J. Schrag, M. Cygler, 2002, Proceedings of the National Academy of Sciences of the United States of America.

The crystal structure of benzenediazonium tetrafluoroborate, C6H5N2+•BF4−1

M. Cygler, M. Przybylska, R. Elofson, 1982 .

Sterically crowded five-membered heterocyclic systems. Part 2. Conformation of 2,2,5,5-tetramethylpyrrolidin-3-ol from nuclear magnetic resonance spectroscopy, crystal structure, and molecular mechanics calculations

M. Cygler, R. B. Nazarski, R. Skowroński, 1985 .

The Structure of calnexin, an ER chaperone involved in quality control of protein folding.

D. Y. Thomas, Y. Li, J. Schrag, 2001, Molecular cell.

Conformational features of acyclonucleosides: structure of acyclovir, an antiherpes agent

M. Cygler, D. Shugar, G. Birnbaum, 1984 .

The structure of the RlmB 23S rRNA methyltransferase reveals a new methyltransferase fold with a unique knot.

Allan Matte, Miroslaw Cygler, A. Matte, 2002, Structure.

The occluding loop in cathepsin B defines the pH dependence of inhibition by its propeptide.

M. Cygler, T. Fox, J. Sivaraman, 1999, Biochemistry.

Crystallographic Trapping of the Glutamyl-CoA Thioester Intermediate of Family I CoA Transferases*

A. Matte, M. Cygler, E. Rangarajan, 2005, Journal of Biological Chemistry.

Selectivity of lipases : Conformational analysis of suggested intermediates in ester hydrolysis of chiral primary and secondary alcohols

J. Schrag, M. Cygler, J. Zuegg, 1997 .

Lipases and alpha/beta hydrolase fold.

J. Schrag, M. Cygler, Miroslaw Cygler, 1997, Methods in enzymology.

The 3-D structure of a folate-dependent dehydrogenase/cyclohydrolase bifunctional enzyme at 1.5 A resolution.

R. Mackenzie, Y. Li, M. Cygler, 1998, Structure.

Advances in structural understanding of lipases.

J. Schrag, M. Cygler, F. Ergan, 1992, Biotechnology & Genetic Engineering Reviews.

Structure as basis for understanding interfacial properties of lipases.

J. Schrag, M. Cygler, 1997, Methods in enzymology.

Substrate Binding Site and the Role of the FLAP Loop in Candida rugosa Lipase, A Close Relative of Acetylcholinesterase

J. Schrag, M. Cygler, P. Grochulski, 1995 .

Structural determinants defining common stereoselectivity of lipases toward secondary alcohols.

J. Schrag, M. Cygler, P. Grochulski, 1995, Canadian journal of microbiology.

Two conformational states of Candida rugosa lipase

J. Schrag, M. Cygler, P. Grochulski, 1994, Protein science : a publication of the Protein Society.

Structure and conformational flexibility of Candida rugosa lipase.

J. Schrag, M. Cygler, 1999, Biochimica et biophysica acta.

Insights into interfacial activation from an open structure of Candida rugosa lipase.

B Rubin, Y. Li, J. Schrag, 1994, The Journal of biological chemistry.

The alpha/beta hydrolase fold.

S J Remington, I Silman, D. Ollis, 1992, Protein engineering.

Crystallization and structural details of Ca(2+)-induced conformational changes in the EF-hand domain VI of calpain.

M. Cygler, P. Grochulski, H. Blanchard, 2002, Methods in molecular biology.

Structure of a calpain Ca2+-binding domain reveals a novel EF-hand and Ca2+-induced conformational changes

M. Cygler, P. Davies, P. Grochulski, 1997, Nature Structural Biology.

Site-directed Mutagenesis of the Active Site Region in the Quinate/Shikimate 5-Dehydrogenase YdiB of Escherichia coli*

R. Ménard, A. Matte, H. Lindner, 2005, Journal of Biological Chemistry.

Progress in understanding the assembly process of bacterial O-antigen.

M. Cygler, R. Morona, S. Kalynych, 2014, FEMS microbiology reviews.

Crystal structure of a trimeric form of dephosphocoenzyme A kinase from Escherichia coli

L. Hung, A. Matte, M. Cygler, 2003, Protein science : a publication of the Protein Society.

Recognition of a cell-surface oligosaccharide of pathogenic Salmonella by an antibody Fab fragment.

M. Cygler, D. Rose, D. Bundle, 1991, Science.

Expression and Characterization of Geotrichum candidum Lipase I Gene

David Y. Thomas, T. Vernet, J. Schrag, 1995 .

Polysaccharide co-polymerases: the enigmatic conductors of the O-antigen assembly orchestra.

M. Cygler, M. Valvano, S. Kalynych, 2012, Protein engineering, design & selection : PEDS.

Structural Characterization of Closely Related O-antigen Lipopolysaccharide (LPS) Chain Length Regulators*

M. Cygler, S. Kalynych, D. Yao, 2012, The Journal of Biological Chemistry.

Sequence-structure relationships in polysaccharide co-polymerase (PCP) proteins.

Allan Matte, Miroslaw Cygler, A. Tocilj, 2009, Trends in biochemical sciences.

Bacterial polysaccharide co-polymerases share a common framework for control of polymer length

J. Rubinstein, A. Tocilj, A. Matte, 2008, Nature Structural &Molecular Biology.

Structure of human procathepsin L reveals the molecular basis of inhibition by the prosegment.

R. Ménard, R. Coulombe, M. Cygler, 1996, The EMBO journal.

Structural and enzymatic characterization of NanS (YjhS), a 9‐O‐Acetyl N‐acetylneuraminic acid esterase from Escherichia coli O157:H7

J. Schrag, M. Cygler, M. Gilbert, 2011, Protein science : a publication of the Protein Society.

Crystal structure of D‐ribose‐5‐phosphate isomerase (RpiA) from Escherichia coli

A. Matte, M. Cygler, E. Rangarajan, 2002, Proteins.

Sindbis virus core protein crystals.

M. Rossmann, M. Luo, P. Dumas, 1989, Journal of molecular biology.

Structure and conformation of 8-bromo-9-.beta.-D-xylofuranosyladenine in the solid state and in solution

I. Ekiel, M. Cygler, D. Shugar, 1982 .

Salmonella Effectors SseK1 and SseK3 Target Death Domain Proteins in the TNF and TRAIL Signaling Pathways*

Nichollas E. Scott, M. Cygler, J. Pearson, 2019, Molecular & Cellular Proteomics.

Insights into function of PSI domains from structure of the Met receptor PSI domain.

G. Kozlov, K. Gehring, J. Schrag, 2004, Biochemical and biophysical research communications.

Crystal structure of chondroitin AC lyase, a representative of a family of glycosaminoglycan degrading enzymes.

M. Cygler, B. Eggimann, J. Féthière, 1999, Journal of molecular biology.

The Architecture of the Multisubunit TRAPP I Complex Suggests a Model for Vesicle Tethering

B. Oh, T. Walz, Y. Kim, 2006, Cell.

Identification and crystallization of a protease-resistant core of calnexin that retains biological activity.

D. Tessier, D. Y. Thomas, J. Schrag, 1998, Journal of structural biology.

Domain organization and crystal structure of the catalytic domain of E.coli RluF, a pseudouridine synthase that acts on 23S rRNA.

A. Matte, M. Cygler, S. Sunita, 2006, Journal of molecular biology.

Structural and mechanistic classification of uronic acid-containing polysaccharide lyases.

Miroslaw Cygler, M. Cygler, M. Garron, 2010, Glycobiology.

Identification of an Escherichia coli O157:H7 heme oxygenase with tandem functional repeats.

Z. Jia, A. Matte, M. Cygler, 2005, Proceedings of the National Academy of Sciences of the United States of America.

Genetic selection designed to stabilize proteins uncovers a chaperone called Spy

Miroslaw Cygler, Ursula Jakob, Philipp Koldewey, 2011, Nature Structural &Molecular Biology.

Insights from protein-protein interaction studies on bacterial pathogenesis

S. Phanse, M. Babu, Alla Gagarinova, 2017, Expert review of proteomics.

15. Structure Information

Miroslaw Cygler, Allan Matte and, Joseph D. Schrag, 2008 .

Structure of rat procathepsin B: model for inhibition of cysteine protease activity by the proregion.

R. Coulombe, M. Cygler, P. Grochulski, 1996, Structure.

Bacterial structural genomics initiative: overview of methods and technologies applied to the process of structure determination.

Allan Matte, Miroslaw Cygler, John Wagner, 2008, Methods in molecular biology.

Salmonella effector SopD promotes plasma membrane scission by inhibiting Rab10

S. Grinstein, B. Raught, É. Coyaud, 2021, Nature Communications.

Structural biology of the invasion arsenal of Gram‐negative bacterial pathogens

Alla Gagarinova, M. Cygler, A. Grishin, 2021, The FEBS journal.

Disulfide Bonds Play a Critical Role in the Structure and Function of the Receptor-binding Domain of the SARS-CoV-2 Spike Antigen

M. Cygler, Olivier Fisette, Shelby Landreth, 2021, Journal of Molecular Biology.

High-resolution crystal structure of Arthrobacter aurescens chondroitin AC lyase: an enzyme-substrate complex defines the catalytic mechanism.

R. Linhardt, M. Cygler, V. Lunin, 2004, Journal of molecular biology.

New Ulvan-Degrading Polysaccharide Lyase Family: Structure and Catalytic Mechanism Suggests Convergent Evolution of Active Site Architecture.

Miroslaw Cygler, Ehud Banin, M. Cygler, 2017, ACS chemical biology.

The Structure of Chondroitin B Lyase Complexed with Glycosaminoglycan Oligosaccharides Unravels a Calcium-dependent Catalytic Machinery*

Robert J Linhardt, Traian Sulea, Rahul Raman, 2004, Journal of Biological Chemistry.

NleH defines a new family of bacterial effector kinases.

S. Phanse, M. Babu, M. Cygler, 2014, Structure.

Molecular basis for the binding and modulation of V-ATPase by a bacterial effector protein

J. Rubinstein, V. Kanelis, M. Cygler, 2017, PLoS pathogens.

Contribution of Structural Genomics to Understanding the Biology of Escherichia coli

Z. Jia, A. Matte, K. Gehring, 2003, Journal of bacteriology.

Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP

Allan Matte, Miroslaw Cygler, Joseph D. Schrag, 2002, Nature Structural Biology.

1.8 A refined structure of the lipase from Geotrichum candidum.

J. Schrag, M. Cygler, J D Schrag, 1993, Journal of molecular biology.

Spike protein disulfide disruption as a potential treatment for SARS-CoV-2

M. Cygler, D. Falzarano, G. George, 2021, bioRxiv.

Disulfide Bonds Play a Critical Role in the Structure and Function of the SARS-CoV-2 Spike Antigen

M. Cygler, Olivier Fisette, Shelby Landreth, 2022 .

Crystal structure of human grancalcin, a member of the penta-EF-hand protein family.

M. Cygler, J. Jia, N. Borregaard, 2000, Journal of molecular biology.

RECOMMENDATIONS FOR NOMENCLATURE IN CHOLINESTERASES

J. Sussman, P. Taylor, I. Silman, 1992 .

Recognition of a carbohydrate antigenic determinant of Salmonella by an antibody.

M. Cygler, D. Rose, D. Bundle, 1993, Biochemical Society transactions.

Cloning and expression of Geotrichum candidum lipase II gene in yeast. Probing of the enzyme active site by site-directed mutagenesis.

D. Tessier, T. Vernet, D. Y. Thomas, 1993, The Journal of biological chemistry.

Creation of a ribonuclease abzyme through site-directed mutagenesis

M. Cygler, Jeremy S. Lee, A. Kuderová, 1998, Nature Biotechnology.

Design of a data model for developing laboratory information management and analysis systems for protein production

Li Xueli, Wayne Boucher, David I Stuart, 2004, Proteins.

Crystal structure of two new bifunctional nonsubstrate type thrombin inhibitors complexed with human α‐thrombin

Y. Konishi, R. Coulombe, M. Cygler, 1996, Protein science : a publication of the Protein Society.

Structure–function analyses of a PL24 family ulvan lyase reveal key features and suggest its catalytic mechanism

M. Cygler, W. Helbert, E. Banin, 2018, The Journal of Biological Chemistry.

Structural basis of the regulation of the CbpA co-chaperone by its specific modulator CbpM.

M. O’Connor-McCourt, J. Baardsnes, I. Ekiel, 2010, Journal of molecular biology.

Structure of the noncatalytic domains and global fold of the protein disulfide isomerase ERp72.

Greg L. Hura, David Y. Thomas, G. Kozlov, 2009, Structure.

Crystal structure of the bb' domains of the protein disulfide isomerase ERp57.

David Y. Thomas, G. Kozlov, K. Gehring, 2006, Structure.

Structure of a single-chain antibody variable domain (Fv) fragment complexed with a carbohydrate antigen at 1.7-A resolution.

Y. Li, C. MacKenzie, M. Cygler, 1994, Proceedings of the National Academy of Sciences of the United States of America.

The Legionella kinase LegK7 exploits the Hippo pathway scaffold protein MOB1A for allostery and substrate phosphorylation

M. Cygler, C. Onu, M. Machner, 2020, Proceedings of the National Academy of Sciences.

Regulation of Shigella Effector Kinase OspG through Modulation of Its Dynamic Properties.

D. Tieleman, M. Cygler, G. Shaw, 2018, Journal of molecular biology.

Structural basis for the inhibition of host protein ubiquitination by Shigella effector kinase OspG.

M. Cygler, G. Shaw, C. Parsot, 2014, Structure.

Structural Snapshots of Escherichia coli Histidinol Phosphate Phosphatase along the Reaction Pathway*

A. Matte, M. Cygler, E. Rangarajan, 2006, Journal of Biological Chemistry.

Crystal structure of histidinol phosphate aminotransferase (HisC) from Escherichia coli, and its covalent complex with pyridoxal-5'-phosphate and l-histidinol phosphate.

A. Matte, Y. Li, J. Schrag, 2001, Journal of molecular biology.

Pancreatic lipases: evolutionary intermediates in a positional change of catalytic carboxylates?

F. Winkler, J. Schrag, M. Cygler, 1992, The Journal of biological chemistry.

Specificity determinants of human cathepsin s revealed by crystal structures of complexes.

Traian Sulea, Miroslaw Cygler, Robert Ménard, 2003, Biochemistry.

Lectin control of protein folding and sorting in the secretory pathway.

David Y. Thomas, J. Schrag, M. Cygler, 2003, Trends in biochemical sciences.

The crystal structure of CREG, a secreted glycoprotein involved in cellular growth and differentiation.

A. Di Bacco, G. Gill, M. Cygler, 2005, Proceedings of the National Academy of Sciences of the United States of America.

The Structure of the MAPK Scaffold, MP1, Bound to Its Partner, p14

M. Cygler, M. Sacher, V. Lunin, 2004, Journal of Biological Chemistry.

Structure of apoptosis-linked protein ALG-2: insights into Ca2+-induced changes in penta-EF-hand proteins.

M. Cygler, J. Jia, M. Berchtold, 2001, Structure.

A structural genomics pilot project based on gene targets selected from Escherichia coli

T. Gaasterland, A. Matte, J. Schrag, 2001 .

The crystal structure of Escherichia coli MoeA, a protein from the molybdopterin synthesis pathway.

A. Matte, J. Schrag, M. Cygler, 2001, Journal of molecular biology.

Crystal structure of human procathepsin X: a cysteine protease with the proregion covalently linked to the active site cysteine.

R. Ménard, M. Cygler, J. Sivaraman, 2000, Journal of molecular biology.

Crystallization and preliminary x‐ray diffraction studies of human procathepsin L

R. Ménard, R. Coulombe, M. Cygler, 1996, Proteins.

Restriction of intramolecular movements within the Cry1Aa toxin molecule of Bacillus thuringiensis through disulfide bond engineering

J. Schwartz, M. Cygler, L. Masson, 1997, FEBS letters.

Composite active site of chondroitin lyase ABC accepting both epimers of uronic acid.

M. Cygler, Yeong Shik Kim, Joon-Soo Sim, 2008, Glycobiology.

Crystal structure of the complex of human α‐thrombin and nonhydrolyzable bifunctional inhibitors, hirutonin—2 and hirutonin—6

Y Konishi, Y. Konishi, B. Edwards, 1993, Proteins.

Solution structure of a trisaccharide-antibody complex: comparison of NMR measurements with a crystal structure.

J. Brisson, M. Cygler, S. Gagné, 1994, Biochemistry.

Legionella pneumophila effector Lem4 is a membrane-associated protein tyrosine phosphatase

Alla Gagarinova, M. Cygler, Lei Li, 2018, The Journal of Biological Chemistry.

Salmonella effectors SseK1 and SseK3 target death domain proteins in the TNF and TRAIL signaling pathways

Nichollas E. Scott, M. Cygler, J. Pearson, 2018, bioRxiv.

Structural insight into effector proteins of Gram‐negative bacterial pathogens that modulate the phosphoproteome of their host

M. Cygler, A. Grishin, K. Beyrakhova, 2015, Protein science : a publication of the Protein Society.

Crystal Structure of Escherichia coli PdxA, an Enzyme Involved in the Pyridoxal Phosphate Biosynthesis Pathway*

D. Cane, A. Matte, M. Cygler, 2003, Journal of Biological Chemistry.

Lysine-based Structure Responsible for Selective Mannose Phosphorylation of Cathepsin D and Cathepsin L Defines a Common Structural Motif for Lysosomal Enzyme Targeting*

K. Tao, G. Sahagian, M. Cygler, 1998, The Journal of Biological Chemistry.

Crystal Structures of Escherichia coli ATP-Dependent Glucokinase and Its Complex with Glucose

Allan Matte, Miroslaw Cygler, Joseph D. Schrag, 2004, Journal of bacteriology.

CS lyases: structure, activity, and applications in analysis and the treatment of diseases.

R. Linhardt, M. Cygler, Yeong Shik Kim, 2006, Advances in pharmacology.

Crystal structure of Proteus vulgaris chondroitin sulfate ABC lyase I at 1.9A resolution.

Miroslaw Cygler, Nobuo Sugiura, Vladimir V Lunin, 2003, Journal of molecular biology.

An Atypical Approach Identifies TYR234 as the Key Base Catalyst in Chondroitin AC Lyase

S. Withers, A. Matte, M. Cygler, 2006, Chembiochem : a European journal of chemical biology.

Role of arginine 292 in the catalytic activity of chondroitin AC lyase from Flavobacterium heparinum.

A. Matte, R. Linhardt, M. Cygler, 2002, Biochimica et biophysica acta.

Preparation, characterization and crystallization of an antibody Fab fragment that recognizes RNA. Crystal structures of native Fab and three Fab-mononucleotide complexes.

Y. Li, M. Cygler, Yunge Li, 1995, Journal of molecular biology.

The Solution Structure of YbcJ from Escherichia coli Reveals a Recently Discovered αL Motif Involved in RNA Binding

K. Gehring, I. Ekiel, M. Cygler, 2003, Journal of bacteriology.

Structure and functional dynamics of the mitochondrial Fe/S cluster synthesis complex

M. Cygler, R. Lill, S. Freibert, 2017, Nature Communications.

The structure of the exopolyphosphatase (PPX) from Escherichia coli O157:H7 suggests a binding mode for long polyphosphate chains.

A. Matte, J. Schrag, M. Cygler, 2006, Journal of molecular biology.

Structural Organization of Enzymes of the Phenylacetate Catabolic Hybrid Pathway

M. Cygler, A. Grishin, 2015, Biology.

Structure of GlgS from Escherichia coli suggests a role in protein–protein interactions

G. Kozlov, K. Gehring, M. Cygler, 2004, BMC Biology.

Sulfonation of glycopeptide antibiotics by sulfotransferase StaL depends on conformational flexibility of aglycone scaffold

T. Sulea, R. Shi, M. Cygler, 2012, Proceedings of the National Academy of Sciences.

Protein-Protein Interactions in the β-Oxidation Part of the Phenylacetate Utilization Pathway

M. Cygler, I. Rouiller, M. Bostina, 2012, The Journal of Biological Chemistry.

Crystal structure of the YciO protein from Escherichia coli

A. Matte, M. Cygler, V. Sauvé, 2002, Proteins.

NMR structure of YcgL, a conserved protein from Escherichia coli representing the DUF709 family, with a novel α/β/α sandwich fold

I. Ekiel, M. Cygler, M. Hung, 2007, Proteins.

Letter to the Editor: 1H, 13C and 15N resonance assignments of human 5,10-methenyltetrahydrofolate synthetase

E. H. Copeland, I. Ekiel, M. Cygler, 2004, Journal of biomolecular NMR.

The KH domain facilitates the substrate specificity and unwinding processivity of DDX43 helicase

M. Cygler, A. Kusalik, V. Vidhyasagar, 2020, The Journal of biological chemistry.

Structural Snapshots of Heparin Depolymerization by Heparin Lyase I*

Zhenqing Zhang, R. Linhardt, M. Cygler, 2009, The Journal of Biological Chemistry.

Expression and characterization of Geotrichum candidum lipase I gene. Comparison of specificity profile with lipase II.

T. Vernet, D. Y. Thomas, J. Schrag, 1995, European journal of biochemistry.

Structure of Ca(2+)-loaded human grancalcin.

M. Cygler, J. Jia, N. Borregaard, 2001, Acta crystallographica. Section D, Biological crystallography.

Crystallization and preliminary X-ray analysis of human grancalcin, a novel cytosolic Ca2+-binding protein present in leukocytes.

Y. Li, M. Cygler, J. Jia, 2000, Acta crystallographica. Section D, Biological crystallography.

Conformational flexibility of PL12 family heparinases: structure and substrate specificity of heparinase III from Bacteroides thetaiotaomicron (BT4657)

D. Tieleman, R. Linhardt, R. Shi, 2017, Glycobiology.

Structural Biology Conformational fl exibility of PL 12 family heparinases : structure and substrate speci fi city of heparinase III from Bacteroides thetaiotaomicron ( BT 4657 )

D. Tieleman, R. Linhardt, R. Shi, 2017 .

Crystal Structure of Heparinase II from Pedobacter heparinus and Its Complex with a Disaccharide Product*

A. Tocilj, M. Cygler, R. Sasisekharan, 2006, Journal of Biological Chemistry.

Cloning, Baeyer-Villiger Biooxidations, and Structures of the Camphor Pathway 2-Oxo-Δ3-4,5,5-Trimethylcyclopentenylacetyl-Coenzyme A Monooxygenase of Pseudomonas putida ATCC 17453

R. Shi, M. Cygler, Hannes G. Leisch, 2012, Applied and Environmental Microbiology.

Characterization of interactions between LPS transport proteins of the Lpt system.

J. Baardsnes, M. Cygler, Linhua Zhang, 2011, Biochemical and biophysical research communications.

Interactions between kinase scaffold MP1/p14 and its endosomal anchoring protein p18.

M. Cygler, J. Magee, 2011, Biochemistry.

The final player in the coenzyme A biosynthetic pathway.

M. Cygler, N. O'Toole, 2003, Structure.

Structural and functional study of Legionella pneumophila effector RavA

Alla Gagarinova, M. Cygler, Pengfei Li, 2021, Protein science : a publication of the Protein Society.

Channeling efficiency in the bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase domain: the effects of site-directed mutagenesis of NADP binding residues.

R. Mackenzie, M. Cygler, M. Allaire, 2000, Biochimica et biophysica acta.

Conformation of complementarity determining region L1 loop in murine IgG lambda light chain extends the repertoire of canonical forms.

M. Cygler, M Cygler, S Wu, 1993, Journal of molecular biology.

Structural basis for specificity of papain‐like cysteine protease proregions toward their cognate enzymes

J. Jenkins, R. Coulombe, M. Cygler, 1998, Proteins.

The synthesis and structure of some 4-aryloxymethyl-2,2,6,6-tetramethyl-4-piperidinol and 4-aryloxymethyl-4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy derivatives

M. Cygler, W. Strzyžewski, R. Skowroński, 1986 .

Crystal structures of apo and metal-bound forms of the UreE protein from Helicobacter pylori: role of multiple metal binding sites.

A. Matte, R. Shi, M. Cygler, 2010, Biochemistry.

PEFLINS: A Family of Penta EF-Hand Proteins

M. Cygler, 2006 .

Molecular dynamics-solvated interaction energy studies of protein-protein interactions: the MP1-p14 scaffolding complex.

Marwen Naïm, Traian Sulea, Miroslaw Cygler, 2008, Journal of molecular biology.

Structure of CbpA J-Domain Bound to the Regulatory Protein CbpM Explains Its Specificity and Suggests Evolutionary Link between CbpM and Transcriptional Regulators

J. Baardsnes, R. Shi, I. Ekiel, 2014, PloS one.

Improvement of the Thermostability and Activity of a Pectate Lyase by Single Amino Acid Substitutions, Using a Strategy Based on Melting-Temperature-Guided Sequence Alignment

T. Sulea, M. Cygler, P. Lau, 2007, Applied and Environmental Microbiology.

Redesigning the active site of Geotrichum candidum lipase.

T. Vernet, D. Y. Thomas, J. Schrag, 1995, Protein engineering.

Crystal Structure of Escherichia coli Glucose-1-Phosphate Thymidylyltransferase (RffH) Complexed with dTTP and Mg2+ *

A. Matte, M. Cygler, V. Sauvé, 2002, The Journal of Biological Chemistry.

Trapping open and closed forms of FitE—A group III periplasmic binding protein

Allan Matte, Miroslaw Cygler, Qizhi Cui, 2009, Proteins.

An Indispensable Role for the MavE Effector of Legionella pneumophila in Lysosomal Evasion

M. Cygler, M. Šantić, Y. Abu Kwaik, 2021, mBio.

1.8 ANGSTROMS REFINED STRUCTURE OF THE LIPASE FROM GEOTRICHUM CANDIDUM

J. Schrag, M. Cygler, 1993 .

Structure of the N-terminal domain of the effector protein LegC3 from Legionella pneumophila.

M. Cygler, D. Yao, M. Cherney, 2014, Acta crystallographica. Section D, Biological crystallography.

Structure and active site residues of PglD, an N-acetyltransferase from the bacillosamine synthetic pathway required for N-glycan synthesis in Campylobacter jejuni.

T. Sulea, A. Matte, M. Cygler, 2008, Biochemistry.

Structural and Functional Analysis of Campylobacter jejuni PseG

Allan Matte, Traian Sulea, Miroslaw Cygler, 2009, The Journal of Biological Chemistry.

INSECTICIDAL TOXIN: STRUCTURE AND CHANNEL FORMATION

M. Cygler, P. Grochulski, 1997 .

A case study on the treatment of protein SIRAS data.

M. Cygler, Pu Han, H. Fan, 2014, Acta crystallographica. Section D, Biological crystallography.

Structural and functional characterization of PL28 family ulvan lyase NLR48 from Nonlabens ulvanivorans

M. Cygler, W. Helbert, E. Banin, 2018, The Journal of Biological Chemistry.

Structure and function of the glycopeptide N-methyltransferase MtfA, a tool for the biosynthesis of modified glycopeptide antibiotics.

B. Zakeri, T. Sulea, A. Matte, 2009, Chemistry & biology.

Design of P1' and P3' residues of trivalent thrombin inhibitors and their crystal structures.

Y. Konishi, Y. Li, M. Cygler, 2000, Biochemistry.

Nonpolar interactions of thrombin S' subsites with its bivalent inhibitor: methyl scan of the inhibitor linker.

E. Purisima, Y. Konishi, T. Sulea, 1997, Biochemistry.

Crystallization and structure determination of an autoimmune anti-poly(dT) immunoglobulin Fab fragment at 3.0 A resolution.

A. Boodhoo, M. Cygler, W. Anderson, 1987, The Journal of biological chemistry.

Crystallization and preliminary X-ray analysis of chondroitin sulfate ABC lyases I and II from Proteus vulgaris.

A. Matte, M. Cygler, W. Huang, 2000, Acta crystallographica. Section D, Biological crystallography.

Crystal Structure of the Salmonella Typhimurium Effector GtgE

G. Kozlov, K. Gehring, M. Cygler, 2016, PloS one.

Polysaccharide co-polymerases: the enigmatic conductors of the O-antigen assembly orchestra.

M. Cygler, M. Valvano, S. Kalynych, 2012, Protein engineering, design & selection : PEDS.

The structural genomics experimental pipeline: Insights from global target lists

Miroslaw Cygler, Zbyszek Otwinowski, Wladek Minor, 2004, Proteins.

A data management system for structural genomics

M. Cygler, N. O'Toole, Stéphane Raymond, 2004, Proteome Science.

Enantioselectivity of Candida Rugosa Lipase Toward Carboxylic Acids: A Predictive Rule from Substrate Mapping and X-Ray Crystallography

J. Schrag, M. Cygler, Sharmin N. Ahmed, 1994 .

Application of Dynamic Light Scattering in Protein Crystallization

R. Shi, M. Cygler, A. Proteau, 2010, Current protocols in protein science.

Molecular dynamics-solvated interaction energy studies of protein-protein interactions: the MP1-p14 scaffolding complex.

E. Purisima, T. Sulea, J. Schrag, 2008, Journal of molecular biology.

A. Yee, A. Semesi, G. Kozlov, 2004, Journal of bacteriology.

Crystal structure of a peptidyl pyridinium methyl ketone inhibitor with thrombin.

Y. Konishi, Y. Li, M. Cygler, 1995, Biochemistry.

Structure of the pseudouridine synthase RsuA from Haemophilus influenzae.

S. Burley, G. Louie, A. Matte, 2005, Acta crystallographica. Section F, Structural biology and crystallization communications.

Structure and conformation of the antiviral nucleoside 2'-fluoro-5-iodoarabinosylcytosine (FIAC). The gauche effect in nucleosides

M. Cygler, Kyoichi A. Watanabe, G. Birnbaum, 1982 .

Trapping open and closed forms of FitE—A group III periplasmic binding protein

E. Purisima, A. Matte, R. Shi, 2009, Proteins.

Crystallization and preliminary X-ray diffraction studies of the lepidopteran-specific insecticidal crystal protein CrylA(a).

M. Cygler, P. Grochulski, H. van Faassen, 1994, Journal of molecular biology.