C. Siebold

发表

Crystal structure of the phosphoenolpyruvate-binding enzyme I-domain from the Thermoanaerobacter tengcongensis PEP: sugar phosphotransferase system (PTS).

U. Baumann, A. Oberholzer, M. Bumann, 2005, Journal of molecular biology.

Repulsive Guidance Molecule is a structural bridge between Neogenin and Bone Morphogenetic Protein

C. H. Bell, C. Siebold, E. Healey, 2015, Nature Structural &Molecular Biology.

The structure and function of the outer coat protein VP9 of Banna virus.

D. Stuart, X. de Lamballerie, P. de Micco, 2005, Structure.

MHC class II proteins and disease: a structural perspective

Lars Fugger, Christian Siebold, E. Yvonne Jones, 2006, Nature Reviews Immunology.

Structural basis for integration of GluD receptors within synaptic organizer complexes

M. Yuzaki, K. Kohda, K. Matsuda, 2016, Science.

Repulsive guidance molecules lock growth differentiation factor 5 in an inhibitory complex

T. Mueller, C. Siebold, B. Bishop, 2020, Proceedings of the National Academy of Sciences.

Glypicans shield the Wnt lipid moiety to enable signalling at a distance

E. Jones, C. Siebold, B. Bishop, 2020, Nature.

The morphogen Sonic hedgehog inhibits its receptor Patched by a pincer grasp mechanism

Benjamin Bishop, Mark S. P. Sansom, Tomas Malinauskas, 2019, Nature Chemical Biology.

Explorer Structural and functional studies of LRP 6 ectodomain reveal a platform for Wnt signaling

J. Mao, E. Jones, C. Siebold, 2018 .

Initiation of T cell signaling by CD45 segregation at ‘close-contacts’

D. Klenerman, Steven F. Lee, Kristina A. Ganzinger, 2016, Nature Immunology.

Structural basis for Smoothened regulation by its extracellular domains

M. Sansom, R. Rambo, C. Siebold, 2016, Nature.

Secreted HHIP1 interacts with heparan sulfate and regulates Hedgehog ligand localization and function

C. Siebold, B. Bishop, Benjamin L. Allen, 2015, The Journal of cell biology.

Structure of the Repulsive Guidance Molecule (RGM)–Neogenin Signaling Hub

C. H. Bell, C. Siebold, E. Healey, 2013, Science.

Automation of large scale transient protein expression in mammalian cells

D. Stuart, Weixian Lu, C. Siebold, 2011, Journal of structural biology.

Structural and functional studies of LRP6 ectodomain reveal a platform for Wnt signaling.

J. Mao, E. Jones, C. Siebold, 2011, Developmental cell.

Structural insights into hedgehog ligand sequestration by the human hedgehog-interacting protein HIP

Benjamin Bishop, A. Radu Aricescu, Karl Harlos, 2009, Nature Structural &Molecular Biology.

Crystal structure of HLA-DQ0602 that protects against type 1 diabetes and confers strong susceptibility to narcolepsy

Arne Svejgaard, Lars Fugger, Karl Harlos, 2004, Proceedings of the National Academy of Sciences of the United States of America.

Structure of the fungal β‐glucan‐binding immune receptor dectin‐1: Implications for function

S. Gordon, E. Jones, James Brown, 2007, Protein science : a publication of the Protein Society.

Crystal Structure of Enzyme I of the Phosphoenolpyruvate Sugar Phosphotransferase System in the Dephosphorylated State*

U. Baumann, A. Oberholzer, P. Schneider, 2009, The Journal of Biological Chemistry.

Lrig2 Negatively Regulates Ectodomain Shedding of Axon Guidance Receptors by ADAM Proteases.

C. Hoogenraad, Jeroen A. A. Demmers, T. Yamashita, 2015, Developmental cell.

RGMs: Structural Insights, Molecular Regulation, and Downstream Signaling.

T. Yamashita, C. Siebold, B. Mueller, 2017, Trends in cell biology.

Large spectrum of lissencephaly and pachygyria phenotypes resulting from de novo missense mutations in tubulin alpha 1A (TUBA1A)

D. Keays, J. Flint, C. Fallet-Bianco, 2007, Human mutation.

Cilia-Associated Oxystero ls Activate Smoothened Graphical Abstract Highlights

D. Elnatan, A. Renslo, P. Beachy, 2018 .

Structures of vertebrate Patched and Smoothened reveal intimate links between cholesterol and Hedgehog signalling.

R. E. Woolley, C. Siebold, R. Rohatgi, 2019, Current opinion in structural biology.

Structural insights into semaphorins and their receptors.

E. Jones, C. Siebold, E. Jones, 2013, Seminars in cell & developmental biology.

Mechanism-based Inhibition of Enzyme I of the Escherichia coli Phosphotransferase System

C. Siebold, B. Erni, J. Hewel, 2002, The Journal of Biological Chemistry.

Structural insights into hedgehog ligand sequestration by the human hedgehog-interacting protein HHIP

Jones, C. Siebold, K. Harlos, 2013 .

Multiple ligand binding sites regulate the Hedgehog signal transducer Smoothened in vertebrates.

C. Siebold, R. Rohatgi, G. Luchetti, 2018, Current opinion in cell biology.

Intein-mediated cyclization of a soluble and a membrane protein in vivo: function and stability.

C. Siebold, B. Erni, 2002, Biophysical chemistry.

Patched 1 regulates Smoothened by controlling sterol binding to its extracellular cysteine-rich domain

M. Sansom, R. E. Woolley, C. Siebold, 2022, Science advances.

Oxysterols modulate the Hedgehog signalling pathway by binding a novel site on the membrane protein Smoothened, which may offer new options for the treatment of cancers linked to this pathway.

P. Ingham, A. McMahon, C. Siebold, 2013 .

Humidity control as a strategy for lattice optimization applied to crystals of HLA-A*1101 complexed with variant peptides from dengue virus.

D. Stuart, G. Screaton, E. Jones, 2007, Acta crystallographica. Section F, Structural biology and crystallization communications.

Photochemical Probe Identification of a Small‐Molecule Inhibitor Binding Site in Hedgehog Acyltransferase (HHAT)**

C. Siebold, M. Fuchter, Jake L. Greenfield, 2021, Angewandte Chemie.

Photochemical probe identification of the small-molecule binding site in a mammalian membrane-bound O-acyltransferase

C. Siebold, A. Magee, E. Tate, 2020, bioRxiv.

Acylation-coupled lipophilic induction of polarisation (Acyl-cLIP): a universal assay for lipid transferase and hydrolase enzymes† †Electronic supplementary information (ESI) available: Experimental materials and methods, additional Acyl-cLIP data, chemical structures, hit compound counter-screens,

Edward W. Tate, C. Siebold, A. Magee, 2019, Chemical science.

R-spondins engage heparan sulfate proteoglycans to potentiate WNT signaling

Mitchell Ho, J. Carette, I. Jordens, 2020, eLife.

LipIDens: simulation assisted interpretation of lipid densities in cryo-EM structures of membrane proteins

Anna L. Duncan, M. Sansom, C. Siebold, 2022, bioRxiv.

First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value.

T. Sixma, G. Schneider, J. Sussman, 2006, Acta crystallographica. Section D, Biological crystallography.

High-resolution structure of the catalytic region of MICAL (molecule interacting with CasL), a multidomain flavoenzyme-signaling molecule.

R. Owens, D. Stuart, A. Kolodkin, 2005, Proceedings of the National Academy of Sciences of the United States of America.

Title Structure and function of the smoothened extracellulardomain in vertebrate hedgehog signaling

P. Ingham, C. Siebold, Sigrid Nachtergaele, 2017 .

Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling

P. Ingham, C. Siebold, Sigrid Nachtergaele, 2013, eLife.

Structure and functional analysis of the IGF‐II/IGF2R interaction

J. Wallace, James Brown, A. Denley, 2008, The EMBO journal.

Crystal Structure of the Citrobacter freundii Dihydroxyacetone Kinase Reveals an Eight-stranded α-Helical Barrel ATP-binding Domain*

U. Baumann, C. Siebold, B. Erni, 2003, Journal of Biological Chemistry.

A mechanism of covalent substrate binding in the x-ray structure of subunit K of the Escherichia coli dihydroxyacetone kinase

Christian Siebold, U. Baumann, C. Siebold, 2003, Proceedings of the National Academy of Sciences of the United States of America.

Proteoglycan-Specific Molecular Switch for RPTPσ Clustering and Neuronal Extension

J. Flanagan, Weixian Lu, E. Jones, 2011, Science.

Structure and functional analysis of the IGF‐II/IGF2R interaction

J. Wallace, E. Jones, James Brown, 2008, The EMBO journal.

Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling

C. Siebold, B. Bishop, J. Elegheert, 2021, Cell.

Restoring p53 function in human melanoma cells by inhibiting MDM2 and cyclin B1/CDK1-phosphorylated nuclear iASPP.

S. Knapp, Xin Lu, S. Zhong, 2013, Cancer cell.

Hedgehog-Interacting Protein is a multimodal antagonist of Hedgehog signalling

C. Siebold, B. Bishop, R. Gilbert, 2021, Nature Communications.

Biochemical mechanisms of vertebrate hedgehog signaling

C. Siebold, R. Rohatgi, Jennifer H. Kong, 2019, Development.

Modular mechanism of Wnt signaling inhibition by Wnt inhibitory factor 1

Weixian Lu, E. Jones, C. Siebold, 2011, Nature Structural &Molecular Biology.

Calcium-sensing receptor residues with loss- and gain-of-function mutations are located in regions of conformational change and cause signalling bias

E. Jones, C. Siebold, R. Thakker, 2018, Human molecular genetics.

A calcium-sensing receptor mutation causing hypocalcemia disrupts a transmembrane salt bridge to activate β-arrestin–biased signaling

E. Jones, C. Siebold, R. Thakker, 2018, Science Signaling.

Structural basis of semaphorin–plexin signalling

C. H. Bell, K. Mitchell, E. Jones, 2010, Nature.

Biological Crystallography Structure of Hla-a*0301 in Complex with a Peptide of Proteolipid Protein: Insights into the Role of Hla-a Alleles in Susceptibility to Multiple Sclerosis

L. Fugger, E. Jones, C. Siebold, 2022 .

A Dual Binding Mode for RhoGTPases in Plexin Signalling

C. H. Bell, E. Jones, C. Siebold, 2011, PLoS biology.

Mutations in α-Tubulin Cause Abnormal Neuronal Migration in Mice and Lissencephaly in Humans

Steve D. M. Brown, D. Keays, J. Flint, 2007, Cell.

Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling

C. Siebold, Sigrid Nachtergaele, R. Sircar, 2016, eLife.

Cholesterol activates the G-protein coupled receptor Smoothened to promote morphogenetic signaling

C. Siebold, Sigrid Nachtergaele, R. Sircar, 2016, bioRxiv.

Cilia-Associated Oxysterols Activate Smoothened.

D. Elnatan, A. Renslo, P. Beachy, 2018, Molecular cell.

iASPP mediates p53 selectivity through a modular mechanism fine-tuning DNA recognition

K. Au, Zhu Chen, S. Zhong, 2019, Proceedings of the National Academy of Sciences.

Recording information on protein complexes in an information management system

Benjamin Bishop, Chris Morris, Bill Lin, 2011, Journal of structural biology.

An ion-channel modulator from the saliva of the brown ear tick has a highly modified Kunitz/BPTI structure.

D. Stuart, R. Esnouf, M. Dallas, 2009, Journal of molecular biology.

Evidence for the Specificity for Platelet HPA-1a Alloepitope and the Presenting HLA-DR52a of Diverse Antigen-Specific Helper T Cell Clones from Alloimmunized Mothers1

Wei Zhang, C. Siebold, M. Murphy, 2009, The Journal of Immunology.

Mothers Helper T Cell Clones from Alloimmunized HLA-DR 52 a of Diverse Antigen-Specific HPA-1 a Alloepitope and the Presenting Evidence for the Specificity for Platelet

Wei Zhang, C. Siebold, M. Murphy, 2009 .

University of Southern Denmark Calcium-sensing receptor residues with loss- and gain-of-function mutations are located in regions of conformational change and cause signalling bias Gorvin,

E. Jones, C. Siebold, R. Thakker, 2018 .

Carbohydrate transporters of the bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS)

C. Siebold, B. Erni, K. Flükiger, 2001, FEBS letters.

Biochemical and structural studies of ASPP proteins reveal differential binding to p53, p63, and p73.

Xin Lu, E. Jones, C. Siebold, 2008, Structure.

A tick protein with a modified Kunitz fold inhibits human tryptase.

David I Stuart, Karl Harlos, Christian Siebold, 2007, Journal of molecular biology.

Interactions between Hedgehog proteins and their binding partners come into view.

P. Beachy, D. Leahy, S. Hymowitz, 2010, Genes & development.

Strategy for membrane protein crystallization exemplified with OmpA and OmpX

G. Schulz, A. Pautsch, C. Siebold, 1999, Proteins.

Neuropilins lock secreted semaphorins onto plexins in a ternary signaling complex

M. Z. Cader, E. Jones, C. Siebold, 2012, Nature Structural &Molecular Biology.

Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins

D. Stuart, R. E. Woolley, E. Jones, 2018, Nature Protocols.

Structure of a Tyrosine Phosphatase Adhesive Interaction Reveals a Spacer-Clamp Mechanism

Weixian Lu, E. Jones, C. Siebold, 2007, Science.

Structure, mechanism, and inhibition of Hedgehog acyltransferase

Edward W. Tate, E. Pardon, J. Steyaert, 2021, Molecular cell.

Structure and Mechanism of Hedgehog Acyl Transferase

Edward W. Tate, E. Pardon, J. Steyaert, 2021, bioRxiv.

The Energetics and Ion Coupling of Cholesterol Transport Through Patched1

M. Sansom, C. Siebold, R. Corey, 2023, bioRxiv.

Diversity of oligomerization in Drosophila semaphorins suggests a mechanism of functional fine-tuning

M. Renner, E. Jones, C. Siebold, 2019, Nature Communications.

Structural Plasticity of Eph-Receptor A4 Facilitates Cross-Class Ephrin Signaling

R. Owens, D. Stuart, E. Jones, 2009, Structure.

Structural Plasticity of Eph Receptor A4 Facilitates Cross-Class Ephrin Signaling

R. Owens, D. Stuart, E. Jones, 2009, Structure.

Relative Affinities of Protein–Cholesterol Interactions from Equilibrium Molecular Dynamics Simulations

M. Sansom, C. Siebold, P. Stansfeld, 2021, bioRxiv.

Cholesterol accessibility at the ciliary membrane controls hedgehog signaling

C. Siebold, J. McDonald, R. Rohatgi, 2019, eLife.

Cholesterol accessibility at the ciliary membrane controls

C. Siebold, J. McDonald, R. Rohatgi, 2022 .

Small substrate, big surprise: fold, function and phylogeny of dihydroxyacetone kinases

U. Baumann, A. Oberholzer, C. Siebold, 2006, Cellular and Molecular Life Sciences CMLS.

Structure and function of the ROR2 cysteine-rich domain in vertebrate noncanonical WNT5A signaling

S. Sidhu, C. Siebold, A. Wagner, 2021, bioRxiv.

Crystal structure of the GluR2 amino-terminal domain provides insights into the architecture and assembly of ionotropic glutamate receptors.

E. Jones, C. Siebold, K. Harlos, 2009, Journal of molecular biology.

Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP.

S. Knapp, Xin Lu, S. Zhong, 2016, Cancer cell.

Patched 1 reduces the accessibility of cholesterol in the outer leaflet of membranes

C. Siebold, R. Sircar, R. Rohatgi, 2021, eLife.

Cholesterol access in cellular membranes controls Hedgehog signaling

C. Siebold, R. Rohatgi, A. Radhakrishnan, 2020, Nature Chemical Biology.

Cholesterol accessibility at the ciliary membrane controls 1 Hedgehog signaling 2 3

C. Siebold, J. McDonald, R. Rohatgi, 2019 .

Molecular analysis of receptor protein tyrosine phosphatase μ‐mediated cell adhesion

Christian Siebold, Weixian Lu, E. Jones, 2006, The EMBO journal.

Cholesterol Interaction Sites on the Transmembrane Domain of the Hedgehog Signal Transducer and Class F G Protein-Coupled Receptor Smoothened

M. Chavent, M. Sansom, C. Siebold, 2018, bioRxiv.

Structural insights into proteoglycan-shaped Hedgehog signaling

C. Siebold, R. Gilbert, T. Malinauskas, 2013, Proceedings of the National Academy of Sciences.

A procedure for setting up high‐throughput nanolitre crystallization experiments. Crystallization workflow for initial screening, automated storage, imaging and optimization

Lester G. Carter, L. Carter, R. Owens, 2005, Acta crystallographica. Section D, Biological crystallography.

Rocking the MBOAT: Structural insights into the membrane bound O-acyltransferase family.

M. Sansom, C. Siebold, T. Ansell, 2023, Current opinion in structural biology.

Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins

D. Stuart, R. E. Woolley, E. Jones, 2018, Nature Protocols.

Receptor protein tyrosine phosphatase micro: measuring where to stick.

E. Jones, C. Siebold, A. Aricescu, 2008, Biochemical Society transactions.

Sphingomyelin suppresses Hedgehog signaling by restricting cholesterol accessibility at the ciliary membrane

C. Siebold, R. Rohatgi, D. Covey, 2019, bioRxiv.

The Inseparable Relationship Between Cholesterol and Hedgehog Signaling.

C. Siebold, R. Rohatgi, 2023, Annual review of biochemistry.

Author response: Cholesterol accessibility at the ciliary membrane controls hedgehog signaling

C. Siebold, J. McDonald, R. Rohatgi, 2019 .

Mutations in α-Tubulin Cause Abnormal Neuronal Migration in Mice and Lissencephaly in Humans

Steve D. M. Brown, D. Keays, J. Flint, 2007, Cell.

Biological Crystallography Structure of Hla-a*0301 in Complex with a Peptide of Proteolipid Protein: Insights into the Role of Hla-a Alleles in Susceptibility to Multiple Sclerosis

L. Fugger, E. Jones, C. Siebold, 2022 .

The energetics and ion coupling of cholesterol transport through Patched1

M. Sansom, C. Siebold, R. Corey, 2023, Science advances.

Multiple sclerosis: MHC associations and therapeutic implications

L. Fugger, J. Bell, C. Siebold, 2005, Expert Reviews in Molecular Medicine.

Biological Crystallography

T. Sixma, J. Sussman, I. Bertini, 2022 .

RGMs: Structural Insights, Molecular Regulation, and Downstream Signaling.

T. Yamashita, C. Siebold, R. Pasterkamp, 2017, Trends in cell biology.

Molecular mechanism of BMP signal control by Twisted gastrulation

H. Ashe, C. Siebold, C. Baldock, 2024, Nature communications.