I. Kumagai

发表

Contribution of Thr29 to the thermodynamic stability of goat α‐lactalbumin as determined by experimental and theoretical approaches

K. Tsumoto, K. Kuwajima, M. Saito, 2001, Proteins.

Microtubule shuttles on kinesin-coated glass micro-wire tracks

I. Kumagai, W. Hwang, H. Nakazawa, 2014, Biomedical microdevices.

A human antibody fragment with high affinity for biodegradable polymer film.

K. Tsumoto, H. Kondo, I. Kumagai, 2007, Bioconjugate chemistry.

Grafting of material-binding function into antibodies Functionalization by peptide grafting.

K. Tsumoto, I. Kumagai, H. Abe, 2008, Biochemical and biophysical research communications.

Synthesis and expression of a DNA encoding the Fv domain of an anti-lysozyme monoclonal antibody, HyHEL10, in Streptomyces lividans.

K. Tsumoto, K. Watanabe, I. Kumagai, 1993, Gene.

Novel anti-Tn single-chain Fv-Fc fusion proteins derived from immunized phage library and antibody Fc domain.

I. Kumagai, K. Nakamura, Rinpei Niwa, 2010, Anticancer research.

Thermodynamic and kinetic effects of human IgG1 defucosylation on IgG1-FcγRIIIa interaction

Masako Wakitani, K. Shitara, K. Tsumoto, 2006 .

Fucose depletion from human IgG1 oligosaccharide enhances binding enthalpy and association rate between IgG1 and FcgammaRIIIa.

Kouhei Tsumoto, Akira Okazaki, Masako Wakitani, 2004, Journal of molecular biology.

Functional Domain Order of an Anti-EGFR × Anti-CD16 Bispecific Diabody Involving NK Cell Activation

I. Kumagai, A. Kuwahara, T. Nakanishi, 2020, International journal of molecular sciences.

Contribution of Asparagine Residues to the Stabilization of a Proteinaceous Antigen-Antibody Complex, HyHEL-10-Hen Egg White Lysozyme*

Kouhei Tsumoto, Hidemasa Kondo, Akiko Yokota, 2009, The Journal of Biological Chemistry.

Role of Tyr Residues in the Contact Region of Anti-lysozyme Monoclonal Antibody HyHEL10 for Antigen Binding (*)

K Watanabe, K. Tsumoto, K. Watanabe, 1995, The Journal of Biological Chemistry.

Chemically Crosslinked Bispecific Antibodies for Cancer Therapy: Breaking from the Structural Restrictions of the Genetic Fusion Approach

I. Kumagai, H. Nakazawa, M. Umetsu, 2020, International journal of molecular sciences.

Application of 300× enhanced fluorescence on a plasmonic chip modified with a bispecific antibody to a sensitive immunosensor.

I. Kumagai, H. Nakazawa, M. Umetsu, 2013, ACS applied materials & interfaces.

Glycosylation analysis of an aggregated antibody produced by Chinese hamster ovary cells in bioreactor culture.

H. Ohtake, T. Omasa, I. Kumagai, 2014, Journal of bioscience and bioengineering.

Entropically driven MHC class I recognition by human inhibitory receptor leukocyte Ig-like receptor B1 (LILRB1/ILT2/CD85j).

Y. Shirakihara, K. Tsumoto, D. Kohda, 2006, Journal of molecular biology.

Molecular cloning and nucleotide sequence determination of gene encoding Streptomyces subtilisin inhibitor (SSI).

I. Kumagai, S. Taguchi, K. Miura, 1989, Journal of biochemistry.

Study of antibody-antigen interaction through site-directed mutagenesis of the VH region of a hybrid phage-antibody fragment.

I. Kumagai, G. Whitelam, Y. Li, 1996, Protein engineering.

High-throughput cytotoxicity and antigen-binding assay for screening small bispecific antibodies without purification.

I. Kumagai, H. Nakazawa, Teppei Niide, 2018, Journal of bioscience and bioengineering.

Cell-growth control by monomeric antigen: the cell surface expression of lysozyme-specific Ig V-domains fused to truncated Epo receptor.

M. Kawahara, K. Tsumoto, G. Winter, 2000, Journal of immunological methods.

Biomass-binding peptides designed by molecular evolution for efficient degradation of cellulose in biomass by cellulase

A. Ikeuchi, I. Kumagai, H. Nakazawa, 2013 .

Automated Discovery of Protein Functional Units from Amino-acid sequences using Rough-Sets-based Comparative Analysis

Kouhei Tsumoto, S. Tsumoto, Hiroshi Tanaka, 1995 .

A thermostable tRNA (guanosine-2')-methyltransferase from Thermus thermophilus HB27 and the effect of ribose methylation on the conformational stability of tRNA.

K. Watanabe, T. Oshima, I. Kumagai, 1982, The Journal of biological chemistry.

A thermostable Gm-methylase recognizes the tertiary structure of tRNA.

K. Watanabe, T. Oshima, I. Kumagai, 1987, Journal of biochemistry.

Effects of modification of 4-thiouridine in E. coli tRNA(fMet) on its methyl acceptor activity by thermostable Gm-methylases.

K. Watanabe, I. Kumagai, K. Miura, 1989, Journal of Biochemistry (Tokyo).

Crystal structure of the hypothetical protein ST2072 from Sulfolobus tokodaii

I. Tanaka, K. Tsumoto, Yoshikazu Tanaka, 2005, Proteins.

T-cell immunotherapy for human MK-1-expressing tumors using a fusion protein of the superantigen SEA and anti-MK-1 scFv antibody.

M. Kuroki, T. Kudo, I. Kumagai, 2002, Anticancer research.

Target cell-restricted apoptosis induction by 528scFv-TRAIL fusion protein specific for human EGFR and expressed in Escherichia coli.

M. Unno, I. Kumagai, Y. Katayose, 2010, International journal of oncology.

Selective expansion of genetically modified T cells using an antibody/interleukin-2 receptor chimera.

M. Kawahara, K. Tsumoto, Teruyuki Nagamune, 2008, Journal of immunological methods.

A highly effective and stable bispecific diabody for cancer immunotherapy: cure of xenografted tumors by bispecific diabody and T-LAK cells

K. Tsumoto, K. Makabe, M. Unno, 2004, Cancer Immunology, Immunotherapy.

Direct immobilization of gold-binding antibody fragments for immunosensor applications.

I. Kumagai, M. Kaieda, M. Umetsu, 2010, Analytical chemistry.

Rapid and Sensitive Detection of Brain-Derived Neurotrophic Factor with a Plasmonic Chip

I. Kumagai, H. Nakazawa, M. Umetsu, 2013 .

Zinc oxide-coated plasmonic chip modified with a bispecific antibody for sensitive detection of a fluorescent labeled-antigen.

I. Kumagai, M. Umetsu, K. Tawa, 2011, Analytical chemistry.

Association behavior and control of the quality of cancer therapeutic bispecific diabodies expressed in Escherichia coli

Yoshikazu Tanaka, I. Kumagai, H. Nakazawa, 2020 .

Highly Enhanced Cytotoxicity of a Dimeric Bispecific Diabody, the hEx3 Tetrabody*

M. Unno, T. Kudo, I. Kumagai, 2010, The Journal of Biological Chemistry.

Application of the Fc fusion format to generate tag‐free bi‐specific diabodies

M. Unno, T. Kudo, I. Kumagai, 2010, The FEBS journal.

Cytotoxic Enhancement of a Bispecific Diabody by Format Conversion to Tandem Single-chain Variable Fragment (taFv)

I. Kumagai, M. Umetsu, T. Nakanishi, 2010, The Journal of Biological Chemistry.

Integration of PEGylation and refolding for renaturation of recombinant proteins from insoluble aggregates produced in bacteria--application to a single-chain Fv fragment.

I. Kumagai, M. Umetsu, T. Nakanishi, 2010, Journal of bioscience and bioengineering.

Preferential heterodimerization of a bispecific diabody based on a humanized anti-EGFR antibody 528.

M. Unno, T. Kudo, I. Kumagai, 2008, Protein engineering, design & selection : PEDS.

Efficient construction of a diabody using a refolding system: anti-carcinoembryonic antigen recombinant antibody fragment.

K. Tsumoto, K. Makabe, T. Kudo, 2002, Journal of biochemistry.

Highly Effective Recombinant Format of a Humanized IgG-like Bispecific Antibody for Cancer Immunotherapy with Retargeting of Lymphocytes to Tumor Cells*

M. Unno, T. Kudo, I. Kumagai, 2007, Journal of Biological Chemistry.

Compact Seahorse‐Shaped T Cell–Activating Antibody for Cancer Therapy

N. Manabe, Yoshikazu Tanaka, I. Kumagai, 2018, Advanced Therapeutics.

Affinity maturation of humanized anti-epidermal growth factor receptor antibody using a modified phage-based open sandwich selection method

I. Kumagai, M. Umetsu, Kazuki Kobayashi, 2018, Scientific Reports.

Phage library panning against cytosolic fraction of cells using quantitative dot blotting assay: application of selected VH to histochemistry.

K. Tsumoto, I. Kumagai, Michihiro Nakamura, 2002, Journal of immunological methods.

Lysozyme-dependent Proliferation Switch: Hematopoietic and Hybridoma Cell Growth Control with the Use of Antibody/GP130 Chimera

M. Kawahara, K. Tsumoto, Teruyuki Nagamune, 2002 .

Replacing factor-dependency with that for lysozyme: affordable culture of IL-6-dependent hybridoma by transfecting artificial cell surface receptor.

M. Kawahara, K. Tsumoto, K. Kato, 2001, Biotechnology and bioengineering.

Biomimetic engineering of modular bispecific antibodies for biomolecule immobilization.

I. Kumagai, M. Kaieda, M. Umetsu, 2011, Langmuir : the ACS journal of surfaces and colloids.

Bypassing antibiotic selection: positive screening of genetically modified cells with an antigen-dependent proliferation switch.

M. Kawahara, K. Tsumoto, Teruyuki Nagamune, 2003, Nucleic acids research.

Bioassisted capture and release of nanoparticles on nanolithographed ZnO films

I. Kumagai, M. Umetsu, M. Sugiyama, 2011, Nanotechnology.

Humanization of the Bispecific Epidermal Growth Factor Receptor × CD3 Diabody and Its Efficacy as a Potential Clinical Reagent

K. Tsumoto, K. Makabe, M. Unno, 2006, Clinical Cancer Research.

Construction of a circularly connected VHH bispecific antibody (cyclobody) for the desirable positioning of antigen-binding sites.

K. Makabe, I. Kumagai, M. Umetsu, 2019, Biochemical and biophysical research communications.

Multimerization of anti‐(epidermal growth factor receptor) IgG fragments induces an antitumor effect: the case for humanized 528 scFv multimers

I. Kumagai, M. Umetsu, S. Furumoto, 2013, The FEBS journal.

Isolation and partial characterization of SSI-like protease inhibitors from Streptomyces.

I. Kumagai, S. Taguchi, K. Miura, 1992, FEMS microbiology letters.

AMEGA: antigen-mediated genetically modified cell amplification.

M. Kawahara, K. Tsumoto, Teruyuki Nagamune, 2004, JIM - Journal of Immunological Methods.

A mutated superantigen SEA D227A fusion diabody specific to MUC1 and CD3 in targeted cancer immunotherapy for bile duct carcinoma

K. Tsumoto, T. Kudo, I. Kumagai, 2002, Cancer Immunology, Immunotherapy.

Generation of high-producing cell lines by overexpression of cell division cycle 25 homolog A in Chinese hamster ovary cells.

H. Ohtake, T. Omasa, I. Kumagai, 2013, Journal of bioscience and bioengineering.

Human cystatin A is inactivated by engineered truncation. The NH2-terminal region of the cysteine proteinase inhibitor is essential for expression of its inhibitory activity.

T. Itoh, I. Kumagai, K. Miura, 1995, Biochemistry.

Effects of 5-(N-aminohexyl)carbamoyl-2'-deoxyuridine on endonuclease stability and the ability of oligodeoxynucleotide to activate RNase H.

A. Matsuda, I. Kumagai, N. Haginoya, 1997, Nucleic acids research.

Molecular motor-powered shuttles along multi-walled carbon nanotube tracks.

J. Ramón‐Azcón, H. Shiku, T. Matsue, 2013, Nano letters.

Herpes simplex virus targeting to the EGF receptor by a gD-specific soluble bridging molecule.

K. Tsumoto, I. Kumagai, J. Glorioso, 2005, Molecular therapy : the journal of the American Society of Gene Therapy.

In vitro and in vivo antitumor effects of recombinant bispecific antibodies based on humanized anti-EGFR antibody.

M. Unno, T. Kudo, I. Kumagai, 2011, Oncology reports.

Diabody-based Recombinant Formats of Humanized IgG-like Bispecific Antibody With Effective Retargeting of Lymphocytes to Tumor Cells

M. Unno, T. Kudo, I. Kumagai, 2008, Journal of immunotherapy.

Construction of a diabody (small recombinant bispecific antibody) using a refolding system.

K. Tsumoto, T. Kudo, I. Kumagai, 2000, Protein engineering.

Functional Fv fragment of an antibody specific for CD28: Fv‐mediated co‐stimulation of T cells

K. Tsumoto, T. Kudo, I. Kumagai, 2000, FEBS letters.

Rearranging the domain order of a diabody-based IgG-like bispecific antibody enhances its antitumor activity and improves its degradation resistance and pharmacokinetics

M. Unno, T. Omasa, T. Kudo, 2014, mAbs.

Behavior of Kinesin Driven Quantum Dots Trapped in a Microtubule Loop.

I. Kumagai, W. Hwang, H. Nakazawa, 2015, ACS nano.

Evaluation of amnion-derived mesenchymal stem cells for treatment-resistant moderate Crohn’s disease: study protocol for a phase I/II, dual-centre, open-label, uncontrolled, dose–response trial

I. Kumagai, K. Yamahara, N. Sakamoto, 2018, BMJ open gastroenterology.

Crystal Structure of Anti-Hen Egg White Lysozyme Antibody (HyHEL-10) Fv-Antigen Complex

K. Tsumoto, H. Kondo, M. Matsushima, 1999, The Journal of Biological Chemistry.

Cell-free expression of two single-chain monoclonal antibodies against lysozyme: effect of domain arrangement on the expression.

V. Erdmann, W. Stiege, K. Tsumoto, 1999, Journal of biochemistry.

Electrochemical detection of epidermal growth factor receptors on a single living cell surface by scanning electrochemical microscopy.

H. Shiku, T. Matsue, Yasufumi Takahashi, 2009, Analytical chemistry.

Salicylate‐induced translocation of prestin having mutation in the GTSRH sequence to the plasma membrane

H. Wada, K. Tsumoto, I. Kumagai, 2010, FEBS letters.

Humanized bispecific antibodies retargeting of lymphocytes against tumor cells

I. Kumagai, R. Asano, 2008 .

Structural considerations for functional anti-EGFR × anti-CD3 bispecific diabodies in light of domain order and binding affinity

K. Makabe, I. Kumagai, T. Kumagai, 2018, Oncotarget.

Organic crystal-binding peptides: morphology control and one-pot formation of protein-displaying organic crystals.

I. Kumagai, H. Nakazawa, Teppei Niide, 2015, Nanoscale.

Domain order of a bispecific diabody dramatically enhances its antitumor activity beyond structural format conversion: the case of the hEx3 diabody.

M. Unno, T. Kudo, I. Kumagai, 2013, Protein engineering, design & selection : PEDS.

Direct and selective immobilization of proteins by means of an inorganic material-binding peptide: discussion on functionalization in the elongation to material-binding peptide.

K. Tsumoto, I. Kumagai, H. Abe, 2010, The journal of physical chemistry. B.

Solubilization of active green fluorescent protein from insoluble particles by guanidine and arginine.

D. Ejima, K. Tsumoto, T. Arakawa, 2003, Biochemical and biophysical research communications.

A visualization method of filamentous phage infection and phage-derived proteins in Escherichia coli using biotinylated phages.

K. Tsumoto, I. Kumagai, K. Ishimura, 2001, Biochemical and biophysical research communications.

Enhanced bacteriolytic activity of hen egg-white lysozyme due to conversion of Trp62 to other aromatic amino acid residues.

I. Kumagai, K. Miura, 1989, Journal of biochemistry.

Growth control of hybridoma cells with an artificially induced EpoR-gp130 heterodimer

M. Kawahara, K. Tsumoto, Teruyuki Nagamune, 2007, Cytotechnology.

Selection of highly productive mammalian cells based on an inducible growth advantage using an antibody/receptor chimera.

M. Kawahara, K. Tsumoto, Teruyuki Nagamune, 2002, Journal of bioscience and bioengineering.

Mechanism of HSV infection through soluble adapter-mediated virus bridging to the EGF receptor.

M. Kuwano, I. Kumagai, J. Glorioso, 2011, Virology.

A Double Mutation in Glycoprotein gB Compensates for Ineffective gD-Dependent Initiation of Herpes Simplex Virus Type 1 Infection

P. Grandi, I. Kumagai, J. Glorioso, 2010, Journal of Virology.

Effect of tandem repeated AUG codons on translation efficiency of eukaryotic mRNA carrying a short leader sequence

I. Kumagai, K. Miura, I. Hirao, 1993, Molecular and General Genetics MGG.

Microtubule guiding in a multi-walled carbon nanotube circuit

J. Ramón‐Azcón, H. Shiku, T. Matsue, 2015, Biomedical microdevices.

On-column refolding and characterization of soluble human interleukin-15 receptor alpha-chain produced in Escherichia coli.

K. Tsumoto, I. Kumagai, S. Misawa, 2003, Protein expression and purification.

Alteration of the Specificity of the Streptomyces Subtilisin Inhibitor by Gene Engineering

I. Kumagai, K. Miura, S. Kojima, 1990, Bio/Technology.

A new method for the synthesis of oligodeoxyribonucleotides bearing a 5'-terminal phosphate group

M. Sekine, I. Kumagai, K. Miura, 1987 .

A New Method for the Synthesis of Oligodeoxyribonucleotides Bearing a 5′-Terminal Phosphate Group.

M. Sekine, I. Kumagai, K. Miura, 1987 .

Chiral recognition in the binding of helicenediamine to double strand DNA: interactions between low molecular weight helical compounds and a helical polymer.

M. Yamaguchi, I. Kumagai, K. Tsumoto, 2002, Bioorganic & medicinal chemistry.

Efficient Leukocyte Ig-like Receptor Signaling and Crystal Structure of Disulfide-linked HLA-G Dimer*

Kouhei Tsumoto, Daisuke Kohda, K. Tsumoto, 2006, Journal of Biological Chemistry.

Human inhibitory receptors Ig-like transcript 2 (ILT2) and ILT4 compete with CD8 for MHC class I binding and bind preferentially to HLA-G

Kouhei Tsumoto, Sarah Rowland-Jones, Veronique M. Braud, 2003, Proceedings of the National Academy of Sciences of the United States of America.

Expression of a Model Peptide of a Marine Mussel Adhesive Protein in Escherichia coli and Characterization of Its Structural and Functional Properties

K. Tsumoto, I. Kumagai, M. Kitamura, 1999 .

Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide.

Haruo Takahashi, Mitsuo Umetsu, Taiji Ikawa, 2012, Journal of bioscience and bioengineering.

Redesign of the substrate-binding site of hen egg white lysozyme based on the molecular evolution of C-type lysozymes.

I. Kumagai, K. Miura, S. Takeda, 1992, The Journal of biological chemistry.

Structural basis for recognition of the nonclassical MHC molecule HLA-G by the leukocyte Ig-like receptor B2 (LILRB2/LIR2/ILT4/CD85d)

Kouhei Tsumoto, Eiji Kurimoto, Daisuke Kohda, 2006, Proceedings of the National Academy of Sciences.

Functional Expression of Prestin, the Outer Hair Cell Motor Protein, Using the Baculovirus/Insect Cell System

H. Wada, K. Tsumoto, I. Kumagai, 2008 .

Secretory production of chicken ovomucoid domain 3 by Escherichia coli and alteration of inhibitory specificity toward proteases by substitution of the P1 site residue.

I. Kumagai, K. Miura, S. Kojima, 1994, Gene.

Investigation of the recognition of an important uridine in an internal loop of a hairpin ribozyme prepared using post-synthetically modified oligonucleotides.

Y. Komatsu, E. Ohtsuka, I. Kumagai, 1999, Nucleic acids research.

SEA-scFv as a bifunctional antibody: construction of a bacterial expression system and its functional analysis.

K. Tsumoto, Y. Hinoda, T. Kudo, 1999, Biochemical and biophysical research communications.

Substrate Recognition of tRNA (Guanosine-2′-)-methyltransferase from Thermus thermophilus HB27*

I. Kumagai, T. Ueda, K. Nishikawa, 1998, The Journal of Biological Chemistry.

How Additives Influence the Refolding of Immunoglobulin-folded Proteins in a Stepwise Dialysis System

Kouhei Tsumoto, Tadafumi Adschiri, Mitsuo Umetsu, 2003, The Journal of Biological Chemistry.

Human Anti-gold Antibodies

I. Kumagai, M. Umetsu, Hideki Watanabe, 2008, Journal of Biological Chemistry.

Nanoparticles with affinity for biopolymer : Bioassisted room-temperature selective multistacking of inorganic particles on biopolymer film

I. Kumagai, M. Umetsu, Hideki Watanabe, 2008 .

Critical Contribution of Aromatic Rings to Specific Recognition of Polyether Rings

K. Tsumoto, Yoshikazu Tanaka, I. Kumagai, 2008, Journal of Biological Chemistry.

Nondenaturing solubilization of beta2 microglobulin from inclusion bodies by L-arginine.

Shigeki Nitta, Kouhei Tsumoto, Tadafumi Adschiri, 2005, Biochemical and biophysical research communications.

Pathway to Insoluble Aggregates on the Refolding of a Single-chain Fv Antibody : Morphological Changes of Aggregated Protein on Refolding

K. Tsumoto, I. Kumagai, M. Umetsu, 2004 .

Structural characteristics and refolding of in vivo aggregated hyperthermophilic archaeon proteins

K. Tsumoto, Yoshikazu Tanaka, I. Kumagai, 2004, FEBS letters.

Role of Arginine in Protein Refolding, Solubilization, and Purification

Kouhei Tsumoto, Mitsuo Umetsu, Daisuke Ejima, 2004, Biotechnology progress.

Antitumor activity of interleukin‐21 prepared by novel refolding procedure from inclusion bodies expressed in Escherichia coli

K. Tsumoto, K. Makabe, T. Kudo, 2002, FEBS letters.

Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent--application to a human single-chain Fv fragment.

K. Tsumoto, H. Kondo, I. Kumagai, 1998, Journal of immunological methods.

Effects of amino acid substitutions in the hydrophobic core of alpha-lactalbumin on the stability of the molten globule state.

K. Kuwajima, K. Watanabe, I. Kumagai, 1995, Protein engineering.

Effect of the order of antibody variable regions on the expression of the single-chain HyHEL10 Fv fragment in E. coli and the thermodynamic analysis of its antigen-binding properties.

K. Tsumoto, K. Yutani, K. Ogasahara, 1994, Biochemical and biophysical research communications.

Inhibition of the hepatitis C virus NS3 protease activity by Fv fragment of antibody 8D4.

K. Tsumoto, I. Kumagai, N. Kasai, 2001, Biochemical and biophysical research communications.

Effective treatment of an orthotopic xenograft model of human glioblastoma using an EGFR-retargeted oncolytic herpes simplex virus.

P. Grandi, E. Chiocca, M. Kuroki, 2013, Molecular therapy : the journal of the American Society of Gene Therapy.

The Role of Hydrogen Bonding via Interfacial Water Molecules in Antigen-Antibody Complexation

Kouhei Tsumoto, Hidemasa Kondo, Akiko Yokota, 2003, The Journal of Biological Chemistry.

Refolding of therapeutic proteins produced in Escherichia coli as inclusion bodies.

I. Kumagai, S. Misawa, I Kumagai, 1999, Biopolymers.

High Affinity Anti-inorganic Material Antibody Generation by Integrating Graft and Evolution Technologies

Satoshi Ohara, Hiroya Abe, Tadafumi Adschiri, 2009, The Journal of Biological Chemistry.

Cloning and expression of the rubredoxin gene from Desulfovibrio vulgaris (Miyazaki F)--comparison of the primary structure of desulfoferrodoxin.

I. Kumagai, K. Miura, K. Kohno, 1997, Biochimica et biophysica acta.

Electrochemical screening of recombinant protein solubility in Escherichia coli using scanning electrochemical microscopy (SECM).

H. Shiku, T. Matsue, K. Nagamine, 2007, Biotechnology and bioengineering.

Monovalent Antigen Activates Antibody/Cytokine Receptor Chimera and Controls Hematopoietic Cell Growth

M. Kawahara, K. Tsumoto, Teruyuki Nagamune, 2002 .

Comparison of secretory expression in Escherichia coli and Streptomyces of Streptomyces subtilisin inhibitor (SSI) gene.

I. Kumagai, S. Taguchi, K. Miura, 1990, Biochimica et biophysica acta.

Immobilized oxidoreductase as an additive for refolding inclusion bodies: application to antibody fragments.

K. Tsumoto, I. Kumagai, M. Umetsu, 2003, Protein engineering.

Antigen–Antibody Binding

K. Tsumoto, I. Kumagai, 2010 .

Structural Consequences of Target Epitope-directed Functional Alteration of an Antibody

K. Tsumoto, H. Kondo, I. Kumagai, 2003, Journal of Biological Chemistry.

Complementary Design for Pairing between Two Types of Nanoparticles Mediated by a Bispecific Antibody: Bottom-Up Formation of Porous Materials from Nanoparticles.

N. Manabe, I. Kumagai, H. Nakazawa, 2019, Langmuir : the ACS journal of surfaces and colloids.

Tumor-directed lymphocyte-activating cytokines: refolding-based preparation of recombinant human interleukin-12 and an antibody variable domain-fused protein by additive-introduced stepwise dialysis.

K. Tsumoto, K. Makabe, T. Kudo, 2005, Biochemical and biophysical research communications.

Enhancement of cellulolytic enzyme activity by clustering cellulose binding domains on nanoscaffolds.

Haruo Takahashi, Do-Myoung Kim, Ryutaro Asano, 2011, Small.

Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer.

K. Tsumoto, Teruyuki Nagamune, I. Kumagai, 2001, Analytical biochemistry.

Effect of a rare leucine codon, TTA, on expression of a foreign gene in Streptomyces lividans.

I. Kumagai, S. Taguchi, K. Miura, 1993, Biochimica et biophysica acta.

Isolation and Characterization of Monoclonal Antibodies That Inhibit Hepatitis C Virus NS3 Protease

Kouhei Tsumoto, Takamasa Ueno, Nobuhiro Kasai, 2000, Journal of Virology.

Unusual genetic codes and a novel gene structure for tRNA(AGYSer) in starfish mitochondrial DNA.

H. Masaki, K. Watanabe, I. Kumagai, 1987, Gene.

Practical considerations in refolding proteins from inclusion bodies.

Kouhei Tsumoto, Daisuke Ejima, D. Ejima, 2003, Protein expression and purification.

Structural evidence for guanidine-protein side chain interactions: crystal structure of CutA from Pyrococcus horikoshii in 3 M guanidine hydrochloride.

I. Tanaka, K. Tsumoto, T. Arakawa, 2004, Biochemical and biophysical research communications.

High-level expression of porcine muscle adenylate kinase in Escherichia coli: effects of the copy number of the gene and the translational initiation signals.

I. Kumagai, K. Miura, K. Yazaki, 1994, Journal of biotechnology.

Molecular Cloning and Sequencing of cDNA Encoding Goat Pre α-Lactalbumin

I. Kumagai, K. Miura, S. Kakinuma, 1987 .

Structural Consequences of Mutations in Interfacial Tyr Residues of a Protein Antigen-Antibody Complex

K. Tsumoto, Yoshikazu Tanaka, H. Kondo, 2007, Journal of Biological Chemistry.

Expression of a synthetic gene encoding canine milk lysozyme in Escherichia coli and characterization of the expressed protein.

K. Kuwajima, T. Ikura, I. Kumagai, 1999, Protein engineering.

Effect of the extra n-terminal methionine residue on the stability and folding of recombinant alpha-lactalbumin expressed in Escherichia coli.

K. Tsumoto, K. Kuwajima, T. Ikura, 1999, Journal of Molecular Biology.

Comprehensive study of domain rearrangements of single-chain bispecific antibodies to determine the best combination of configurations and microbial host cells

I. Kumagai, K. Sode, R. Asano, 2018, mAbs.

Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of α2,6-sialyltransferase derived from Chinese hamster ovary cells

H. Ohtake, T. Omasa, I. Kumagai, 2012, Applied Microbiology and Biotechnology.

Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells

H. Ohtake, T. Omasa, I. Kumagai, 2009, Applied Microbiology and Biotechnology.

Human CD4+ central and effector memory T cells produce IL-21: effect on cytokine-driven proliferation of CD4+ T cell subsets.

K. Tsumoto, K. Makabe, N. Tanaka, 2007, International immunology.

Human CD 4 1 central and effector memory T cells produce IL-21 : effect on cytokine-driven proliferation of CD 4 1 T cell subsets

K. Tsumoto, K. Makabe, N. Tanaka, 2007 .

Open sandwich ELISA: A novel immunoassay based on the interchain interaction of antibody variable region

Kouhei Tsumoto, Walter C. Mahoney, Eiji Suzuki, 1996, Nature Biotechnology.

Effects of subsite alterations on substrate-binding mode in the active site of hen egg-white lysozyme.

I. Kumagai, K. Miura, S. Takeda, 1993, European journal of biochemistry.

Electrochemical detection of receptor-mediated endocytosis by scanning electrochemical microscopy.

K. Ino, H. Shiku, T. Matsue, 2011, Physical chemistry chemical physics : PCCP.

Engineering the hinge region of human IgG1 Fc-fused bispecific antibodies to improve fragmentation resistance

I. Kumagai, R. Asano, S. Konno, 2018, Scientific Reports.

Affinity maturation of humanized anti-epidermal growth factor receptor antibody using a modified phage-based open sandwich selection method

I. Kumagai, M. Umetsu, Kazuki Kobayashi, 2018, Scientific Reports.

Development of an affinity-matured humanized anti-epidermal growth factor receptor antibody for cancer immunotherapy.

I. Kumagai, M. Umetsu, T. Nakanishi, 2013, Protein engineering, design & selection : PEDS.

Construction and humanization of a functional bispecific EGFR × CD16 diabody using a refolding system

Hiroki Hayashi, Michiaki Unno, Ryutaro Asano, 2012, The FEBS journal.

Thermodynamic Consequences of Mutations in Vernier Zone Residues of a Humanized Anti-human Epidermal Growth Factor Receptor Murine Antibody, 528*

K. Tsumoto, K. Makabe, Yoshikazu Tanaka, 2008, Journal of Biological Chemistry.

Inhibition of hepatitis C virus NS3 protease by peptides derived from complementarity‐determining regions (CDRs) of the monoclonal antibody 8D4: tolerance of a CDR peptide to conformational changes of a target

K. Tsumoto, I. Kumagai, S. Misawa, 2002, FEBS letters.

Structural Evidence for Entropic Contribution of Salt Bridge Formation to a Protein Antigen-Antibody Interaction

K. Tsumoto, K. Yutani, K. Ogasahara, 2001, The Journal of Biological Chemistry.

How Oligomerization Contributes to the Thermostability of an Archaeon Protein

I. Tanaka, K. Tsumoto, H. Fukada, 2004, Journal of Biological Chemistry.

Relationship between utilization of dual translational initiation signals and protein processing in Streptomyces

I. Kumagai, S. Taguchi, K. Miura, 1991, Molecular and General Genetics MGG.

Analysis of transcriptional control regions in the Streptomyces subtilisin-inhibitor-encoding gene.

I. Kumagai, S. Taguchi, K. Miura, 1989, Gene.

Efficient Extracellular Expression of a Foreign Protein in Streptomyces Using Secretory Protease Inhibitor (SSI) Gene Fusions

I. Kumagai, S. Taguchi, K. Miura, 1989, Nature Biotechnology.

Structural analysis of mutant hen egg-white lysozyme preferring a minor binding mode.

K. Watanabe, G. Kawai, M. Matsushima, 1998, Biochimica et biophysica acta.

Antigen-Mediated Genetically Modified Cell Amplification (AMEGA) with Single Vector Transductio

M. Kawahara, K. Tsumoto, Teruyuki Nagamune, 2004 .

High IL-21 receptor expression and apoptosis induction by IL-21 in follicular lymphoma.

K. Makabe, I. Kumagai, Yasuaki Yamada, 2007, Cancer letters.

Reversal of antigen-dependent signaling by two mutations in antibody/receptor chimera: implication of inverse agonism in cytokine receptor superfamily.

M. Kawahara, K. Tsumoto, Teruyuki Nagamune, 2004, Biochemical Pharmacology.

Terminal heterogeneity and corrections of the nucleotide sequence of 5S rRNA from an extreme thermophile, Thermus thermophilus HB8.

I. Kumagai, V. Erdmann, S. Takemura, 1983, Nucleic acids research.

Synthesis of alpha-helix-forming peptides by gene engineering methods and their characterization by circular dichroism spectra measurements.

S. Takahashi, I. Kumagai, K. Miura, 1996, Biochimica et biophysica acta.

Expression and Secretion of Bovine β-Lactoglobulin in Saccharomyces cerevisiae

M. Totsuka, I. Kumagai, K. Miura, 1990 .

Expression and secretion of bovine beta-lactoglobulin in Saccharomyces cerevisiae.

M. Totsuka, I. Kumagai, K. Miura, 1990, Agricultural and biological chemistry.

Mechanisms of temporary inhibition in Streptomyces subtilisin inhibitor induced by an amino acid substitution, tryptophan 86 replaced by histidine.

K. Kanaori, I. Kumagai, K. Miura, 1991, Biochemistry.

Contribution of salt bridge in the protease inhibitor SSI (Streptomyces subtilisin inhibitor) to its inhibitory action

I. Kumagai, K. Miura, K. Fujimura, 1994, FEBS letters.

Molecular recognition at the active site of subtilisin BPN': crystallographic studies using genetically engineered proteinaceous inhibitor SSI (Streptomyces subtilisin inhibitor).

Y. Satow, S. Noguchi, I. Kumagai, 1994, Protein engineering.

Requirement for a disulfide bridge near the reactive site of protease inhibitor SSI (Streptomyces subtilisin inhibitor) for its inhibitory action.

I. Kumagai, K. Miura, S. Kojima, 1993, Journal of molecular biology.

Effects of deletion in the flexible loop of the protease inhibitor SSI (Streptomyces subtilisin inhibitor) on interactions with proteases.

I. Kumagai, K. Miura, I Kumagai, 1993, Protein engineering.

High frequency of SSI-like protease inhibitors among Streptomyces.

I. Kumagai, S. Taguchi, K. Miura, 1993, Bioscience, biotechnology, and biochemistry.

A protease inhibitor produced by Streptomyces lividans 66 exhibits inhibitory activities toward both subtilisin BPN' and trypsin.

K. Tsumoto, I. Kumagai, K. Miura, 1992, Journal of biochemistry.

Inhibition of subtilisin BPN' by reaction site P1 mutants of Streptomyces subtilisin inhibitor.

I. Kumagai, K. Miura, Y. Nishiyama, 1991, Journal of biochemistry.

Effect of inhibitory activity of mutation at reaction site P4 of the Streptomyces subtilisin inhibitor, SSI.

I. Kumagai, K. Miura, S. Kojima, 1990, Protein engineering.

Identification of amino acid residues responsible for the changes of absorption and fluorescence spectra on the binding of subtilisin BPN' and Streptomyces subtilisin inhibitor.

I. Kumagai, K. Inouye, B. Tonomura, 1993, Journal of biochemistry.

Interaction of subtilisin BPN' and recombinant Streptomyces subtilisin inhibitors with substituted P1 site residues.

K. Kanaori, T. Hatanaka, I. Kumagai, 1993, Journal of biochemistry.

Trehalose suppresses antibody aggregation during the culture of Chinese hamster ovary cells.

T. Omasa, I. Kumagai, H. Maseda, 2014, Journal of bioscience and bioengineering.

Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of α2,6-sialyltransferase derived from Chinese hamster ovary cells

H. Ohtake, T. Omasa, I. Kumagai, 2011, Applied Microbiology and Biotechnology.

Dissection of protein-carbohydrate interactions in mutant hen egg-white lysozyme complexes and their hydrolytic activity.

K. Watanabe, M. Matsushima, I. Kumagai, 1995, Journal of molecular biology.

Mimicry of erythropoietin and interleukin-6 signalling by an antibody/cytokine receptor chimera in murine myeloid 32D cells.

M. Kawahara, K. Tsumoto, Teruyuki Nagamune, 2007, Journal of biochemistry.

High-level expression in Streptomyces lividans 66 of a gene encoding Streptomyces subtilisin inhibitor from Streptomyces albogriseolus S-3253.

I. Kumagai, K. Miura, S. Obata, 1989, Journal of biochemistry.

Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27.

K. Watanabe, T. Oshima, I. Kumagai, 1980, Proceedings of the National Academy of Sciences of the United States of America.

Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells.

K. Tsumoto, I. Kumagai, H. Ueda, 2010, Journal of immunological methods.

Evaluation of intercellular cross-linking abilities correlated with cytotoxicities of bispecific antibodies with domain rearrangements using AFM force-sensing.

Hyonchol Kim, I. Kumagai, K. Ishibashi, 2021, Biosensors & bioelectronics.

Fluorolabeling of antibody variable domains with green fluorescent protein variants: application to an energy transfer-based homogeneous immunoassay.

K. Tsumoto, Teruyuki Nagamune, I. Kumagai, 2000, Protein engineering.

Expression and secretion of goat α-lactalbumin as an active protein in Saccharomyces cerevisiae

I. Kumagai, K. Miura, S. Takeda, 1990 .

Role of Salt Bridge Formation in Antigen-Antibody Interaction

K. Tsumoto, K. Yutani, K. Ogasahara, 1996, The Journal of Biological Chemistry.

An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation.

K. Tsumoto, Teruyuki Nagamune, I. Kumagai, 2003, Journal of immunological methods.

The nucleotide sequence of 5S rRNA from an extreme thermophile, Thermus thermophilus HB8.

K. Watanabe, T. Oshima, I. Kumagai, 1981, Nucleic acids research.

Phylogenetic Diversity Reflected in the Three Dimensional Structure of Ribosomal 5S RNA

V. Erdmann, T. Pieler, I. Kumagai, 1982 .

Anti-EGFR antibody 528 binds to domain III of EGFR at a site shifted from the cetuximab epitope

K. Tsumoto, K. Makabe, Yoshikazu Tanaka, 2021, Scientific Reports.

A high-affinity gold-binding camel antibody: antibody engineering for one-pot functionalization of gold nanoparticles as biointerface molecules.

I. Kumagai, M. Umetsu, T. Nakanishi, 2012, Bioconjugate chemistry.

Contribution to antibody-antigen interaction of structurally perturbed antigenic residues upon antibody binding.

K Watanabe, K. Tsumoto, K. Watanabe, 1994, The Journal of biological chemistry.

Structural and functional properties of hen egg-white lysozyme deamidated by protein engineering.

I. Kumagai, A. Kato, S. Tanimoto, 1992, Bioscience, biotechnology and biochemistry.

Critical contribution of VH–VL interaction to reshaping of an antibody: The case of humanization of anti‐lysozyme antibody, HyHEL‐10

Kouhei Tsumoto, Hidemasa Kondo, Akiko Yokota, 2008, Protein science : a publication of the Protein Society.

Anti‐EGFR scFv tetramer (tetrabody) with a stable monodisperse structure, strong anticancer effect, and a long in vivo half‐life

I. Kumagai, M. Umetsu, S. Furumoto, 2016, FEBS open bio.

Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library.

K. Tsumoto, I. Kumagai, K. Ishimura, 2001, Journal of Biochemistry (Tokyo).

Selection of human antibody fragments on the basis of stabilization of the variable domain in the presence of target antigens.

Kouhei Tsumoto, Yoshiyuki Nishimiya, Ryutaro Asano, 2002, Biochemical and biophysical research communications.

Detection of biotinylated proteins in polyacrylamide gels using an avidin-fluorescein conjugate.

K. Tsumoto, I. Kumagai, Michihiro Nakamura, 2002, Analytical biochemistry.

A semi high-throughput method for screening small bispecific antibodies with high cytotoxicity

I. Kumagai, H. Nakazawa, Teppei Niide, 2017, Scientific Reports.

Hybrid Nanocellulosome Design from Cellulase Modules on Nanoparticles: Synergistic Effect of Catalytically Divergent Cellulase Modules on Cellulose Degradation Activity

Hikaru Nakazawa, Mitsuo Umetsu, A. Ikeuchi, 2013 .

A nanocluster design for the construction of artificial cellulosomes

Haruo Takahashi, Do-Myoung Kim, Hikaru Nakazawa, 2012 .

P1 specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor.

I. Kumagai, K. Miura, H. Matsuzawa, 1998, Bioscience, biotechnology, and biochemistry.

Partial Alteration of a Protein Streptomyces Subtilisin Inhibitor by Site-Directed Mutagenesis

I. Kumagai, S. Taguchi, K. Miura, 1988 .

Isolation and Characterization of Monoclonal Antibodies That Inhibit Hepatitis C Virus NS 3 Protease

K. Tsumoto, I. Kumagai, S. Misawa, 2000 .

Homogeneous noncompetitive immunoassay based on the energy transfer between fluorolabeled antibody variable domains (open sandwich fluoroimmunoassay).

K. Tsumoto, Teruyuki Nagamune, I. Kumagai, 1999, BioTechniques.

Imaging by Atomic Force Microscopy of the Plasma Membrane of Prestin-Transfected Chinese Hamster Ovary Cells

T. Gomi, H. Wada, K. Tsumoto, 2006, Journal of the Association for Research in Otolaryngology.

Protein–protein interactions and selection: generation of molecule‐binding proteins on the basis of tertiary structural information

I. Kumagai, M. Umetsu, T. Nakanishi, 2010, The FEBS journal.

A growth signal with an artificially induced erythropoietin receptor-gp130 cytoplasmic domain heterodimer.

M. Kawahara, K. Tsumoto, Teruyuki Nagamune, 2001, Journal of biochemistry.

Functional construction of the anti-mucin core protein (MUC1) antibody MUSE11 variable regions in a bacterial expression system.

K. Tsumoto, T. Kudo, I. Kumagai, 2000, Journal of biochemistry.

Importance of the hydrogen bonding network including Asp52 for catalysis, as revealed by Asn59 mutant hen egg-white lysozymes.

M. Matsushima, I. Kumagai, K. Maenaka, 2009, Journal of biochemistry.

Thermodynamic Consequences of Grafting Enhanced Affinity toward the Mutated Antigen onto an Antibody

K. Tsumoto, K. Yutani, I. Kumagai, 2000, The Journal of Biological Chemistry.

Material-binding peptide application--ZnO crystal structure control by means of a ZnO-binding peptide.

Satoshi Ohara, Takashi Naka, Hiroya Abe, 2011, Journal of bioscience and bioengineering.

The effect of an agglutogen on virus infection: biotinylated filamentous phages and avidin as a model

K. Tsumoto, I. Kumagai, Michihiro Nakamura, 2002, FEBS Letters.

Construction of mono- and bivalent human single-chain Fv fragments against the D antigen in the Rh blood group: multimerization effect on cell agglutination and application to blood typing.

K. Tsumoto, I. Kumagai, M. Uchikawa, 1998, Protein engineering.

The effect of a cytidine-to-uridine transition on the stability of Escherichia coli A19 5-S RNA.

V. Erdmann, T. Pieler, I. Kumagai, 2005, European journal of biochemistry.

Engineering the hinge region of human IgG1 Fc-fused bispecific antibodies to improve fragmentation resistance

I. Kumagai, R. Asano, Saori Suzuki, 2018, Scientific Reports.

Molecular properties of two proteins homologous to PduO‐type ATP:cob(I)alamin adenosyltransferase from Sulfolobus tokodaii

I. Tanaka, K. Tsumoto, Yoshikazu Tanaka, 2007, Proteins.

Structural implications for heavy metal‐induced reversible assembly and aggregation of a protein: the case of Pyrococcus horikoshii CutA 1

I. Tanaka, K. Tsumoto, Yoshikazu Tanaka, 2004, FEBS letters.

Nucleotide sequence and secondary structure analysis of spinach chloroplast 4.5 S RNA.

V. Erdmann, T. Pieler, I. Kumagai, 1982, Journal of Biological Chemistry.

Folding‐unfolding of goat α‐lactalbumin studied by stopped‐flow circular dichroism and molecular dynamics simulations

K. Tsumoto, K. Kuwajima, M. Saito, 2001, Proteins.

Functional conversion of the homologous proteins alpha-lactalbumin and lysozyme by exon exchange.

I. Kumagai, K. Miura, S. Takeda, 1992, Proceedings of the National Academy of Sciences of the United States of America.

The primary structure of pre-cytochrome c3 from Desulfovibrio vulgaris (Miyazaki F) as determined by nucleotide sequencing of its gene and partial amino acid sequencing

I. Kumagai, K. Miura, K. Ozawa, 1993 .

Novel selection method for engineered antibodies using the mechanism of Fv fragment stabilization in the presence of antigen.

K. Tsumoto, Teruyuki Nagamune, K. Yutani, 1997, Protein engineering.

A Double Mutation in Glycoprotein gB Compensates for Ineffective gD-Dependent Initiation of Herpes Simplex Virus Type 1 Infection

P. Grandi, I. Kumagai, J. Glorioso, 2010, Journal of Virology.

Effects of Mutation in the Conserved GTSRH Sequence of the Motor Protein Prestin on Its Characteristics

H. Wada, K. Tsumoto, I. Kumagai, 2005 .

Glycosylation analysis of an aggregated antibody produced by Chinese hamster ovary cells in bioreactor culture.

H. Ohtake, T. Omasa, I. Kumagai, 2014, Journal of bioscience and bioengineering.

Biomimetic engineering of modular bispecific antibodies for biomolecule immobilization.

I. Kumagai, M. Umetsu, Takeshi Imamura, 2011, Langmuir : the ACS journal of surfaces and colloids.

Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing

K. Makabe, I. Kumagai, H. Nakazawa, 2017, Protein engineering, design & selection : PEDS.

Phage-display selection of antibodies to the left end of CTX3C using synthetic fragments.

K. Tsumoto, I. Kumagai, M. Mizugaki, 2004, Journal of immunological methods.

HETEROGENEOUS EXPRESSION OF THE MOTOR PROTEIN PRESTIN OF COCHLEAR OUTER HAIR CELLS

H. Wada, I. Kumagai, Koji Iida, 2006 .

Construction of an expression system for the motor protein prestin in Chinese hamster ovary cells

H. Wada, K. Tsumoto, I. Kumagai, 2005, Hearing Research.

Relationship between Fluorescence Intensity of GFP and the Expression Level of Prestin in a Prestin-Expressing Chinese Hamster Ovary Cell Line

H. Wada, K. Tsumoto, I. Kumagai, 2004 .

Stable Expression of the Motor Protein Prestin in Chinese Hamster Ovary Cells

H. Wada, K. Tsumoto, I. Kumagai, 2003 .

Purification of the Motor Protein Prestin from Chinese Hamster Ovary Cells Stably Expressing Prestin

H. Wada, K. Tsumoto, I. Kumagai, 2008 .

Quantum Dot Motion on Microtubules

I. Kumagai, W. Hwang, M. Umetsu, 2012 .

Build-up functionalization of anti-EGFR × anti-CD3 bispecific diabodies by integrating high-affinity mutants and functional molecular formats

T. Omasa, I. Kumagai, M. Umetsu, 2020, Scientific Reports.

Nucleotide sequence and secondary structure analysis of spinach chloroplast 4.5 S RNA.

V. Erdmann, T. Pieler, I. Kumagai, 1982, The Journal of biological chemistry.

Impact in stability during sequential CDR grafting to construct camelid VHH antibodies against zinc oxide and gold

K. Makabe, I. Kumagai, H. Nakazawa, 2018, Journal of biochemistry.

Novel FMN-binding protein from Desulfovibrio vulgaris (Miyazaki F). Cloning and expression of its gene in Escherichia coli.

I. Kumagai, K. Miura, H. Akutsu, 1994, The Journal of biological chemistry.

Obsevation of unfolding straucturral changes of goat α-lactalbumin by molecular dynamics simulations at high temperature

K. Tsumoto, K. Kuwajima, M. Saito, 1999 .

Expression of goat alpha-lactalbumin in Escherichia coli and its refolding to biologically active protein.

I. Kumagai, K. Miura, S. Takeda, 1990, Protein engineering.

In vitro and in vivo antitumor effects of recombinant bispecific antibodies based on humanized anti-EGFR antibody.

M. Unno, T. Kudo, I. Kumagai, 2011, Oncology reports.

A morphologic study of filamentous phage infection of Escherichia coli using biotinylated phages

K. Tsumoto, I. Kumagai, Michihiro Nakamura, 2003, FEBS letters.