M. Iwakura

发表

Stabilization of Hyperactive Dihydrofolate Reductase by Cyanocysteine-mediated Backbone Cyclization*

Munehito Arai, Hisashi Takahashi, M. Arai, 2007, Journal of Biological Chemistry.

Systematic circular permutation of an entire protein reveals essential folding elements

Tsutomu Nakamura, K. Maki, M. Iwakura, 2000, Nature Structural Biology.

Dihydrofolate reductase as a new "affinity handle".

Y. Shimura, M. Iwakura, Y. Tanaka, 1992, Journal of biochemistry.

A novel method for peptide block synthesis using unprotected peptides

Y. Ishihama, Y. Oda, T. Takenawa, 1999 .

Purification and characterization of the flagellar hook–basal body complex of Bacillus subtilis

M. Iwakura, T. Kubori, N. Kobayashi, 1997, Molecular microbiology.

Synthesis and antibacterial activity of copolymers having a quaternary ammonium salt side group

M. Iwakura, K. Kaeriyama, T. Tashiro, 1989 .

Stability and reversibility of thermal denaturation are greatly improved by limiting terminal flexibility of Escherichia coli dihydrofolate reductase.

M. Iwakura, S. Honda, 1996, Journal of biochemistry.

Effects of the length of a glycine linker connecting the N-and C-termini of a circularly permuted dihydrofolate reductase.

T. Nakamura, M. Iwakura, 1998, Protein engineering.

SAXS of the chemically denatured states of homologous dihydrofolate reductases.

C. Matthews, O. Bilsel, K. Maki, 2000 .

Apparent radii of the native, stable intermediates and unfolded conformers of the alpha-subunit of tryptophan synthase from E. coli, a TIM barrel protein.

J. Lee, C. Matthews, O. Bilsel, 1999, Biochemistry.

Effects of point mutations at the flexible loop alanine-145 of Escherichia coli dihydrofolate reductase on its stability and function.

K. Gekko, M. Iwakura, E. Ohmae, 1998, Journal of biochemistry.

Enzyme-inhibition system for identifying potential antimalarials that target highly drug-resistant mutants of Plasmodium falciparum dihydrofolate reductase

T. Horii, M. Iwakura, F. Itoh, 1998 .

Circular Permutation Analysis as a Method for Distinction of Functional Elements in the M20 Loop of Escherichia coliDihydrofolate Reductase*

M. Iwakura, Tsutomu Nakamura, M Iwakura, 1999, The Journal of Biological Chemistry.

Discrimination of a single base change in a ribozyme using the gene for dihydrofolate reductase as a selective marker in Escherichia coli.

S. Fujita, M. Iwakura, K. Taira, 1997, Proceedings of the National Academy of Sciences of the United States of America.

An approach for protein to be completely reversible to thermal denaturation even at autoclave temperatures.

T. Takenawa, M. Iwakura, Y. Mitsuishi, 2001, Protein engineering.

Microsecond Hydrophobic Collapse in the Folding of Escherichia coli Dihydrofolate Reductase, an α/β-Type Protein

E. Kondrashkina, C. Matthews, O. Bilsel, 2007 .

Production of hetero-dimeric dihydrofolate reductase-thymidylate synthase bifunctional enzyme.

M. Iwakura, T. Kokubu, 1995, Journal of Biochemistry (Tokyo).

A cross-section of the fitness landscape of dihydrofolate reductase.

Y Husimi, M. Iwakura, Y. Husimi, 2001, Protein engineering.

Evolutional Design of a Hyperactive Cysteine- and Methionine-free Mutant of Escherichia coli Dihydrofolate Reductase*

Akiko Yokota, K. Gekko, K. Maki, 2006, Journal of Biological Chemistry.

Antibacterial activity of copolymers of trialkyl(4-vinylbenzyl)ammonium chlorides with acrylonitrile

M. Iwakura, K. Kaeriyama, Y. Shimura, 1993 .

ATP-dependent proteases degrade their substrates by processively unraveling them from the degradation signal.

A. Matouschek, M. Iwakura, Cheolju Lee, 2001, Molecular cell.

Nano-Scale Monitoring of the Thermally-Induced Unfolding of Proteins Using Capillary Electrophoresis with In-Column Incubation

Y. Ishihama, Y. Oda, M. Iwakura, 1997 .

Collapse of parallel folding channels in dihydrofolate reductase from Escherichia coli by site-directed mutagenesis.

C. Matthews, B. Jones, M. Iwakura, 1993, Biochemistry.

Detection of a stable intermediate in the thermal unfolding of a cysteine-free form of dihydrofolate reductase from Escherichia coli.

C. Matthews, J. Luo, M. Iwakura, 1995, Biochemistry.

Systematic alanine insertion reveals the essential regions that encode structure formation and activity of dihydrofolate reductase

M. Iwakura, H. Kamikubo, M. Kataoka, 2011, Biophysics.

Effects of the difference in the unfolded-state ensemble on the folding of Escherichia coli dihydrofolate reductase.

C. Matthews, K. Kuwajima, M. Arai, 2003, Journal of molecular biology.

Testing the relationship between foldability and the early folding events of dihydrofolate reductase from Escherichia coli.

Munehito Arai, K. Maki, Hisashi Takahashi, 2003, Journal of molecular biology.

Engineering of histidine tail in the N-terminal region of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens

M. Iwakura, A. Suemori, M. Ozawa, 2001, Biotechnology Letters.

A Systematic and Comprehensive Combinatorial Approach to Simultaneously Improve the Activity, Reaction Specificity, and Thermal Stability of p-Hydroxybenzoate Hydroxylase*

M. Iwakura, A. Suemori, 2007, Journal of Biological Chemistry.

Detection of conformational changes in an immobilized protein using surface plasmon resonance.

H. Sota, M. Iwakura, H Sota, 1998, Analytical chemistry.

Thermal Stability of Dihydrofolate Reductase and Its Fused Proteins with Oligopeptides

H. Uedaira, M. Iwakura, S. Kidokoro, 1990, Annals of the New York Academy of Sciences.

Peptide fragment studies on the folding elements of dihydrofolate reductase from Escherichia coli

Munehito Arai, M. Arai, M. Iwakura, 2005, Proteins.

Dihydrofolate reductase gene as a versatile expression marker.

T. Tanaka, M. Iwakura, 1992, Journal of biochemistry.

Probing the interactions between the folding elements early in the folding of Escherichia coli dihydrofolate reductase by systematic sequence perturbation analysis.

Munehito Arai, M. Arai, M. Iwakura, 2005, Journal of molecular biology.

Expression and purification of growth hormone-releasing factor with the aid of dihydrofolate reductase handle.

S. Ohashi, M. Iwakura, T. Kokubu, 1992, Journal of biochemistry.

Microsecond subdomain folding in dihydrofolate reductase.

C. Matthews, O. Bilsel, M. Arai, 2011, Journal of molecular biology.

Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: implication of hydrophobic interactions.

K. Gekko, M. Iwakura, E. Ohmae, 2005, Journal of biochemistry.

Protein oxidation during long storage: identification of the oxidation sites in dihydrofolate reductase from Escherichia coli through LC-MS and fragment studies.

Hisashi Takahashi, T. Takenawa, M. Iwakura, 2009, Journal of biochemistry.

Sequence perturbation analysis: addressing amino acid indices to elucidate the C-terminal role of Escherichia coli dihydrofolate reductase.

Hisashi Takahashi, T. Takenawa, M. Iwakura, 2009, Journal of biochemistry.

Folding Elements of Proteins

M. Arai, M. Iwakura, 2005 .

Site-specific immobilization of molecularly engineered dihydrofolate reductase to gold surfaces

Fumio Mizutani, F. Mizutani, M. Iwakura, 1994 .

Thermal transition of a mutated dihydrofolate reductase

H. Uedaira, M. Iwakura, S. Kidokoro, 1990 .

In search of circular permuted variants of Escherichia coli dihydrofolate reductase.

M. Iwakura, 1998, Bioscience, biotechnology, and biochemistry.