Masayuki Kusano

发表

Engineering, expression, purification, and production of recombinant thermolysin.

K. Inouye, Masayuki Kusano, Y. Hashida, 2007, Biotechnology annual review.

Extracellular production of recombinant thermolysin expressed in Escherichia coli, and its purification and enzymatic characterization.

K. Inouye, Masayuki Kusano, Y. Hashida, 2006, Protein expression and purification.

Effects of the mutational combinations on the activity and stability of thermolysin.

K. Inouye, Masayuki Kusano, K. Yasukawa, 2010, Journal of biotechnology.

Insights into the catalytic roles of the polypeptide regions in the active site of thermolysin and generation of the thermolysin variants with high activity and stability.

K. Inouye, Masayuki Kusano, K. Yasukawa, 2008, Journal of biochemistry.

Engineering of the pH-dependence of thermolysin activity as examined by site-directed mutagenesis of Asn112 located at the active site of thermolysin.

K. Inouye, Masayuki Kusano, Y. Hashida, 2006, Journal of biochemistry.

Characterization of Gly-D-Phe, Gly-L-Leu, and D-Phe as affinity ligands to thermolysin.

K. Inouye, Masayuki Kusano, K. Yasukawa, 2006, Protein expression and purification.

A new method for the extracellular production of recombinant thermolysin by co-expressing the mature sequence and pro-sequence in Escherichia coli.

K. Inouye, Masayuki Kusano, K. Yasukawa, 2007, Protein engineering, design & selection : PEDS.

Synthesis of N-carbobenzoxy-l-aspartyl-l-phenylalanine methyl ester catalyzed by thermolysin variants with improved activity

K. Inouye, Masayuki Kusano, K. Yasukawa, 2010 .

Insights into the catalytic roles of the polypeptide regions in the active site of thermolysin and generation of the thermolysin variants with high activity and stability.

K. Inouye, Masayuki Kusano, K. Yasukawa, 2008, Journal of biochemistry.