Hiroyuki Nakai

发表

A Novel Metabolic Pathway for Glucose Production Mediated by α-Glucosidase-catalyzed Conversion of 1,5-Anhydrofructose*

M. Okuyama, W. Saburi, H. Mori, 2012, The Journal of Biological Chemistry.

M. Okuyama, W. Saburi, H. Mori, 2008 .

[Review: Symposium on Applied Glycoscience] Discovery of Novel Phosphorylases Involved in Nigeran Metabolism from Clostridium phytofermentans

K. Ohtsubo, Hiroyuki Nakai, M. Kitaoka, 2014 .

Amino Acids in Conserved Region II Are Crucial to Substrate Specificity, Reaction Velocity, and Regioselectivity in the Transglucosylation of Honeybee GH-13 α-Glucosidases

M. Okuyama, H. Mori, A. Kimura, 2012, Bioscience, biotechnology, and biochemistry.

Glucoamylase Originating from Schwanniomyces occidentalis Is a Typical α-Glucosidase

M. Okuyama, H. Mori, A. Kimura, 2005 .

Glucoamylase originating from Schwanniomyces occidentalis is a typical alpha-glucosidase.

M. Okuyama, H. Mori, A. Kimura, 2005, Bioscience, biotechnology, and biochemistry.

Using Carbohydrate Interaction Assays to Reveal Novel Binding Sites in Carbohydrate Active Enzymes

B. Svensson, Hiroyuki Nakai, D. Cockburn, 2016, PloS one.

1,2-β-Oligoglucan Phosphorylase from Listeria innocua

Hiroyuki Nakai, M. Kitaoka, M. Nakajima, 2014, PloS one.

Efficient Enzymatic Synthesis of α-(1→4)-glucosidic Disaccharides by Maltose Phosphorylase from Lactobacillus acidophilus NCFM

B. Svensson, Hiroyuki Nakai, T. Nihira, 2011 .

Discovery of solabiose phosphorylase and its application for enzymatic synthesis of solabiose from sucrose and lactose

W. Saburi, H. Mori, Hiroyuki Nakai, 2022, Scientific reports.

3-O-α-D-glucopyranosyl-L-rhamnose phosphorylase from Clostridium phytofermentans.

Hiroyuki Nakai, M. Kitaoka, T. Nihira, 2012, Carbohydrate research.

Identification of Bacillus selenitireducens MLS10 maltose phosphorylase possessing synthetic ability for branched α-D-glucosyl trisaccharides.

Hiroyuki Nakai, M. Kitaoka, T. Nihira, 2012, Carbohydrate research.

Enzymatic synthesis of branched α-D-glucosyl trisaccharides by novel maltose phosphorylase from Bacillus selenitireducens MLS 10

Hiroyuki Nakai, M. Kitaoka, T. Nihira, 2022 .

Analysis of Surface Binding Sites (SBS) within GH62, GH13, and GH77

B. Svensson, R. Field, Hiroyuki Nakai, 2015 .

Starch‐binding domains in the CBM45 family – low‐affinity domains from glucan, water dikinase and α‐amylase involved in plastidial starch metabolism

Diana Santelia, S. Zeeman, B. Svensson, 2011, The FEBS journal.

Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes.

Spencer J. Williams, T. Pons, R. Pickersgill, 2018, Glycobiology.

Multiple forms of α-glucosidase in rice seeds (Oryza sativa L., var Nipponbare)

M. Okuyama, H. Mori, A. Kimura, 2007 .

A novel β-1,2-glucanase from fungi

F. Sugawara, Hiroyuki Nakai, M. Nakajima, 2019 .

[Mini Review] Production of Novel Oligosaccharides by Using of Synthetic Reaction Catalyzing by Carbohydrate Active Enzyme

Hiroyuki Nakai, M. Kitaoka, T. Nihira, 2012 .

Discovery of Two β-1,2-Mannoside Phosphorylases Showing Different Chain-Length Specificities from Thermoanaerobacter sp. X-514

K. Ohtsubo, E. Suzuki, Hiroyuki Nakai, 2014, PloS one.

Synthesis of three deoxy-sophorose derivatives for evaluating the requirement of hydroxy groups at position 3 and/or 3' of sophorose by 1,2-β-oligoglucan phosphorylases.

Hiroyuki Nakai, M. Nakajima, W. Tsuzuki, 2018, Carbohydrate research.

A Solanum torvum GH3 β-glucosidase expressed in Pichia pastoris catalyzes the hydrolysis of furostanol glycoside.

M. Okuyama, A. Kimura, Hiroyuki Nakai, 2016, Phytochemistry.

2-O-α-D-Glucosylglycerol Phosphorylase from Bacillus selenitireducens MLS10 Possessing Hydrolytic Activity on β-D-Glucose 1-Phosphate

K. Ohtsubo, Hiroyuki Nakai, M. Kitaoka, 2014, PloS one.

Rice α-glucosidase isozymes and isoforms showing different starch granules-binding and -degrading ability

M. Okuyama, H. Mori, A. Kimura, 2008 .

Purification and characterization of a chloride ion-dependent alpha-glucosidase from the midgut gland of Japanese scallop (Patinopecten yessoensis)

M. Okuyama, H. Mori, A. Kimura, 2019 .

Purification and characterization of a chloride ion-dependent α-glucosidase from the midgut gland of Japanese scallop (Patinopecten yessoensis)

M. Okuyama, W. Saburi, H. Mori, 2016, Bioscience, biotechnology, and biochemistry.

Cleavage of α-1,4-glycosidic linkages by the glycosylphosphatidylinositol-anchored α-amylase AgtA decreases the molecular weight of cell wall α-1,3-glucan in Aspergillus oryzae

Masafumi Hidaka, Hiroyuki Nakai, A. Yoshimi, 2022, bioRxiv.

An efficient arabinoxylan-debranching α-l-arabinofuranosidase of family GH62 from Aspergillus nidulans contains a secondary carbohydrate binding site

B. Svensson, B. Kragelund, P. Dupree, 2016, Applied Microbiology and Biotechnology.

An inverting β‐1,2‐mannosidase belonging to glycoside hydrolase family 130 from Dyadobacter fermentans

K. Ohtsubo, E. Suzuki, Hiroyuki Nakai, 2015, FEBS letters.

Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity

M. Okuyama, H. Mori, A. Kimura, 2011, Applied Microbiology and Biotechnology.

Colorimetric determination of β-1,2-glucooligosaccharides for an enzymatic assay using 3-methyl-2-benzothiazolinonehydrazone.

Hiroyuki Nakai, M. Nakajima, H. Taguchi, 2018, Analytical biochemistry.

The measurement of hydrogen activities in molten copper using an oxide protonic conductor

Hiroyuki Nakai, T. Ohashi, N. Fukatsu, 1996 .

Crystal Structure and Substrate Recognition of Cellobionic Acid Phosphorylase, Which Plays a Key Role in Oxidative Cellulose Degradation by Microbes*

Hiroyuki Nakai, M. Kitaoka, S. Fushinobu, 2015, The Journal of Biological Chemistry.

The maltodextrin transport system and metabolism in Lactobacillus acidophilus NCFM and production of novel α‐glucosides through reverse phosphorolysis by maltose phosphorylase

B. Svensson, Martin J. Baumann, Hiroyuki Nakai, 2009, The FEBS journal.

Large-scale Preparation of 1,2-β-Glucan Using 1,2-β-Oligoglucan Phosphorylase

Hiroyuki Nakai, M. Kitaoka, M. Nakajima, 2015 .

One Pot Enzymatic Production of Nigerose from Common Sugar Resources Employing Nigerose Phosphorylase

K. Ohtsubo, Hiroyuki Nakai, M. Nishimoto, 2014 .

Recent development of phosphorylases possessing large potential for oligosaccharide synthesis.

Birte Svensson, Ken'ichi Ohtsubo, K. Ohtsubo, 2013, Current opinion in chemical biology.

Efficient one-pot enzymatic synthesis of alpha-(1-->4)-glucosidic disaccharides through a coupled reaction catalysed by Lactobacillus acidophilus NCFM maltose phosphorylase.

B. Svensson, Hiroyuki Nakai, M. Abou Hachem, 2010, Carbohydrate research.

Potassium ion‐dependent trehalose phosphorylase from halophilic Bacillus selenitireducens MLS10

K. Ohtsubo, Hiroyuki Nakai, M. Kitaoka, 2013, FEBS letters.

Characterization of Two α-1,3-Glucoside Phosphorylases from Clostridium phytofermentans

K. Ohtsubo, Hiroyuki Nakai, M. Nishimoto, 2014 .

Characterization of a laminaribiose phosphorylase from Acholeplasma laidlawii PG-8A and production of 1,3-β-D-glucosyl disaccharides.

Hiroyuki Nakai, M. Nishimoto, M. Kitaoka, 2012, Carbohydrate research.

Discovery of nigerose phosphorylase from Clostridium phytofermentans

Hiroyuki Nakai, M. Kitaoka, T. Nihira, 2012, Applied Microbiology and Biotechnology.

Chemoenzymatic synthesis of nove functional oligosaccharides as prebiotics

K. Ohtsubo, B. Svensson, Hiroyuki Nakai, 2011 .

Aspergillus nidulansα‐galactosidase of glycoside hydrolase family 36 catalyses the formation of α‐galacto‐oligosaccharides by transglycosylation

B. Svensson, Martin J. Baumann, Hiroyuki Nakai, 2010, The FEBS journal.

Identification, characterization, and structural analyses of a fungal endo-β-1,2-glucanase reveal a new glycoside hydrolase family

F. Sugawara, T. Terada, Hiroyuki Nakai, 2019, The Journal of Biological Chemistry.

Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from Clostridium thermocellum.

B. Svensson, Martin J. Baumann, Hiroyuki Nakai, 2010, Biochimie.

Structure of a bacterial α-1,2-glucosidase defines mechanisms of hydrolysis and substrate specificity in GH65 family hydrolases

T. Miyazaki, E. Park, Hiroyuki Nakai, 2021, The Journal of biological chemistry.

Key aromatic residues at subsites +2 and +3 of glycoside hydrolase family 31 α-glucosidase contribute to recognition of long-chain substrates.

B. Svensson, M. Okuyama, H. Mori, 2013, Biochimica et biophysica acta.

Enzymology and Structure of the GH13_31 Glucan 1,6-α-Glucosidase That Confers Isomaltooligosaccharide Utilization in the Probiotic Lactobacillus acidophilus NCFM

B. Svensson, L. Lo Leggio, M. S. Møller, 2012, Journal of bacteriology.

Recombinant production and characterisation of two related GH5 endo-β-1,4-mannanases from Aspergillus nidulans FGSC A4 showing distinctly different transglycosylation capacity.

B. Svensson, Martin J. Baumann, Hiroyuki Nakai, 2011, Biochimica et biophysica acta.

Large-scale preparation of β-1,2-glucan using quite a small amount of sophorose

Hiroyuki Nakai, T. Iwata, M. Nakajima, 2019, Bioscience, biotechnology, and biochemistry.

Function-unknown Glycoside Hydrolase Family 31 Proteins, mRNAs of which were Expressed in Rice Ripening and Germinating Stages, are α-Glucosidase and α-Xylosidase

M. Okuyama, H. Mori, A. Kimura, 2007 .

Enzymatic synthesis of β-xylosyl-oligosaccharides by transxylosylation using two β-xylosidases of glycoside hydrolase family 3 from Aspergillus nidulans FGSC A4.

B. Svensson, Martin J. Baumann, Hiroyuki Nakai, 2011, Carbohydrate research.

In vitro growth of four individual human gut bacteria on oligosaccharides produced by chemoenzymatic synthesis.

T. R. Licht, A. Meyer, B. Svensson, 2013, Food & function.

Characterization and structural analyses of a novel glycosyltransferase acting on the β-1,2-glucosidic linkages

Hiroyuki Nakai, M. Nakajima, K. Kuramochi, 2022, The Journal of biological chemistry.

Biochemical and structural analyses of a bacterial endo-β-1,2-glucanase reveal a new glycoside hydrolase family

Hiroki Matsunaga, Hiroyuki Nakai, M. Nakajima, 2017, The Journal of Biological Chemistry.

Functional and Structural Analysis of a β-Glucosidase Involved in β-1,2-Glucan Metabolism in Listeria innocua

Hiroyuki Nakai, M. Kitaoka, M. Nakajima, 2016, PloS one.

Discovery of β-1,4-d-Mannosyl-N-acetyl-d-glucosamine Phosphorylase Involved in the Metabolism of N-Glycans*

K. Ohtsubo, E. Suzuki, Hiroyuki Nakai, 2013, The Journal of Biological Chemistry.

Next-generation prebiotic promotes selective growth of bifidobacteria, suppressing Clostridioides difficile

Aina Gotoh, S. Maeda, Y. Yamauchi, 2021, Gut microbes.

A Snapshot into the Metabolism of Isomalto-oligosaccharides in Probiotic Bacteria

R. Barrangou, T. Klaenhammer, B. Svensson, 2013 .

Colorimetric Quantification of α-D-Mannose 1-Phosphate

K. Ohtsubo, E. Suzuki, Hiroyuki Nakai, 2013 .

An Enzymatic Colorimetric Quantification of Orthophosphate

Hiroyuki Nakai, M. Nishimoto, M. Kitaoka, 2011 .

An Enzymatic Colorimetric Quanti fi cation of Orthophosphate

Hiroyuki Nakai, M. Nishimoto, M. Kitaoka, 2011 .

Multiple forms of alpha-glucosidase in rice seeds (Oryza sativa L., var Nipponbare).

M. Okuyama, H. Mori, A. Kimura, 2007, Biochimie.

Discovery of cellobionic acid phosphorylase in cellulolytic bacteria and fungi

K. Ohtsubo, Hiroyuki Nakai, M. Nishimoto, 2013, FEBS letters.

Bacteroides thetaiotaomicron VPI‐5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions

M. Okuyama, W. Saburi, H. Mori, 2012, The FEBS journal.

Novel Dextranase Catalyzing Cycloisomaltooligosaccharide Formation and Identification of Catalytic Amino Acids and Their Functions Using Chemical Rescue Approach*

N. Suzuki, R. Suzuki, M. Okuyama, 2012, The Journal of Biological Chemistry.

Purification and Characterization of α-Glucosidase I from Japanese Honeybee (Apis cerana japonica) and Molecular Cloning of Its cDNA

M. Okuyama, H. Mori, A. Kimura, 2006, Bioscience, biotechnology, and biochemistry.

Rational engineering of Lactobacillus acidophilus NCFM maltose phosphorylase into either trehalose or kojibiose dual specificity phosphorylase.

B. Svensson, Hiroyuki Nakai, J. Duus, 2010, Protein engineering, design & selection : PEDS.

Enzymatic synthesis of alkyl α-2-deoxyglucosides by alkyl alcohol resistant α-glucosidase from Aspergillus niger

M. Okuyama, W. Saburi, H. Mori, 2005 .

Mechanistic insight into the substrate specificity of 1,2-β-oligoglucan phosphorylase from Lachnoclostridium phytofermentans

Hiroyuki Nakai, M. Nakajima, S. Fushinobu, 2017, Scientific Reports.

Structural and thermodynamic insights into β-1,2-glucooligosaccharide capture by a solute-binding protein in Listeria innocua

T. Terada, Hiroyuki Nakai, M. Nakajima, 2018, The Journal of Biological Chemistry.

Novel glycoside hydrolase family enzymes from Escherichia coli are associated with osmo-regulated periplasmic glucan synthesis

Hiroyuki Nakai, M. Nakajima, Kaito Kobayashi, 2023, bioRxiv.

Enzymatic control and evaluation of degrees of polymerization of β-(1 → 2)-glucans.

Hiroyuki Nakai, T. Iwata, M. Nakajima, 2021, Analytical biochemistry.

Characterization and Structural Analysis of a Novel exo-Type Enzyme Acting on β-1,2-Glucooligosaccharides from Parabacteroides distasonis.

Hiroyuki Nakai, M. Nakajima, N. Sugimoto, 2018, Biochemistry.

Function and structure relationships of a β‐1,2‐glucooligosaccharide‐degrading β‐glucosidase

Hiroyuki Nakai, M. Nakajima, N. Sugimoto, 2017, FEBS letters.

Alkoxycarbonyl elimination of 3-O-substituted glucose and fructose by heat treatment under neutral pH.

Hiroyuki Nakai, M. Kitaoka, H. Ono, 2020, Carbohydrate research.

Structural basis for reversible phosphorolysis and hydrolysis reactions of 2-O-α-glucosylglycerol phosphorylase.

Hiroyuki Nakai, M. Kitaoka, S. Fushinobu, 2014, The Journal of biological chemistry.

Discovery of solabiose phosphorylase and its application for enzymatic synthesis of solabiose from sucrose and lactose

W. Saburi, H. Mori, Hiroyuki Nakai, 2022, Scientific Reports.

Discovery of solabiose phosphorylase and its application for enzymatic synthesis of solabiose from sucrose and lactose

W. Saburi, H. Mori, Hiroyuki Nakai, 2021, Scientific reports.

An efficient arabinoxylan-debranching α-l-arabinofuranosidase of family GH62 from Aspergillus nidulans contains a secondary carbohydrate binding site

B. Svensson, B. Kragelund, P. Dupree, 2016, Applied Microbiology and Biotechnology.

Discovery of nigerose phosphorylase from Clostridium phytofermentans

Hiroyuki Nakai, M. Kitaoka, T. Nihira, 2011, Applied Microbiology and Biotechnology.

In vitro growth of four individual human gut bacteria on oligosaccharides produced by chemoenzymatic synthesis.

T. R. Licht, A. Meyer, B. Svensson, 2013, Food & function.

Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes.

Spencer J. Williams, T. Pons, R. Pickersgill, 2018, Glycobiology.