H. Hemmi

发表

An active-site mutation causes enhanced reactivity and altered regiospecificity of transglucosylation catalyzed by the Bacillus sp. SAM1606 alpha-glucosidase.

T. Nishino, T. Nakayama, M. Inohara-Ochiai, 2000, Journal of bioscience and bioengineering.

Biochemical Analysis of a Novel Lipolytic Enzyme YvdO from Bacillus subtilis 168

H. Hemmi, T. Yoshimura, S. Kato, 2010, Bioscience, biotechnology, and biochemistry.

Identification of genes affecting lycopene formation in Escherichia coli transformed with carotenoid biosynthetic genes: candidates for early genes in isoprenoid biosynthesis.

T. Nishino, H. Hemmi, S. Ohnuma, 1998, Journal of biochemistry.

Alterations in D-amino acid concentrations and microbial community structures during the fermentation of red and white wines.

H. Hemmi, T. Yoshimura, S. Kato, 2011, Journal of bioscience and bioengineering.

Microbial diversity in biodegradation and reutilization processes of garbage.

S. Haruta, M. Ishii, Y. Igarashi, 2005, Journal of bioscience and bioengineering.

Domain characterization of Bacillus subtilis GabR, a pyridoxal 5'-phosphate-dependent transcriptional regulator.

H. Hemmi, T. Yoshimura, M. Goto, 2015, Journal of biochemistry.

UDP-glucuronic Acid:Anthocyanin Glucuronosyltransferase from Red Daisy (Bellis perennis) Flowers

T. Nishino, T. Nakayama, H. Hemmi, 2005, Journal of Biological Chemistry.

Structural and Kinetic Evidence for an Extended Hydrogen-bonding Network in Catalysis of Methyl Group Transfer

S. Ragsdale, H. Hemmi, T. Doukov, 2007, Journal of Biological Chemistry.

Substrate specificity of undecaprenyl diphosphate synthase from the hyperthermophilic archaeon Aeropyrum pernix.

H. Hemmi, T. Ogawa, T. Yoshimura, 2013, Biochemical and biophysical research communications.

Introduction of the archaebacterial geranylgeranyl pyrophosphate synthase gene into Chlamydomonas reinhardtii chloroplast.

E. Fukusaki, A. Kobayashi, T. Nishino, 2003, Journal of bioscience and bioengineering.

Mevalonate Pathway in Bacteria and Archaea

Shunji Takahashi, H. Hemmi, T. Kuzuyama, 2010 .

An alternative mechanism of product chain-length determination in type III geranylgeranyl diphosphate synthase.

T. Nishino, T. Nakayama, H. Hemmi, 2003, European journal of biochemistry.

Change of product specificity of hexaprenyl diphosphate synthase from Sulfolobus solfataricus by introducing mimetic mutations.

T. Nishino, T. Nakayama, H. Hemmi, 2002, Biochemical and biophysical research communications.

Novel Medium-Chain Prenyl Diphosphate Synthase from the Thermoacidophilic Archaeon Sulfolobus solfataricus

T. Nishino, H. Hemmi, S. Yamashita, 2002, Journal of bacteriology.

The role of histidine‐114 of Sulfolobus acidocaldarius geranylgeranyl diphosphate synthase in chain‐length determination

T. Nishino, H. Hemmi, K. Hirooka, 2000, FEBS letters.

Geranylgeranyl Reductase and Ferredoxin from Methanosarcina acetivorans Are Required for the Synthesis of Fully Reduced Archaeal Membrane Lipid in Escherichia coli Cells

H. Hemmi, T. Ogawa, T. Yoshimura, 2013, Journal of bacteriology.

Archaeal Phospholipid Biosynthetic Pathway Reconstructed in Escherichia coli

H. Hemmi, T. Yoshimura, Keisuke Isobe, 2012, Archaea.

Structure and mutation analysis of archaeal geranylgeranyl reductase.

Masahiro Fujihashi, Kunio Miki, Tohru Yoshimura, 2011, Journal of molecular biology.

A novel geranylgeranyl reductase from the methanogenic archaeon Methanosarcina acetivorans displays unique regiospecificity

H. Hemmi, T. Ogawa, T. Yoshimura, 2014, The FEBS journal.

Conversion of Product Specificity of Archaebacterial Geranylgeranyl-diphosphate Synthase

T. Nishino, H. Hemmi, K. Hirooka, 1996, The Journal of Biological Chemistry.

(S)-2,3-Di-O-geranylgeranylglyceryl Phosphate Synthase from the Thermoacidophilic Archaeon Sulfolobus solfataricus

T. Nishino, T. Nakayama, H. Hemmi, 2004, Journal of Biological Chemistry.

Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus

T. Nishino, T. Nakayama, H. Hemmi, 2007, The FEBS journal.

Specific Partial Reduction of Geranylgeranyl Diphosphate by an Enzyme from the Thermoacidophilic Archaeon Sulfolobus acidocaldarius Yields a Reactive Prenyl Donor, Not a Dead-End Product

H. Hemmi, T. Yoshimura, Sho Sato, 2008, Journal of bacteriology.

Crystal structure of mevalonate 3,5-bisphosphate decarboxylase reveals insight into the evolution of decarboxylases in the mevalonate metabolic pathways

J. Bowie, M. Sawaya, D. Cascio, 2022, The Journal of biological chemistry.

Mechanism of eukaryotic serine racemase-catalyzed serine dehydration.

H. Hemmi, T. Yoshimura, M. Goto, 2020, Biochimica et biophysica acta. Proteins and proteomics.

A heteromeric cis-prenyltransferase is responsible for the biosynthesis of glycosyl carrier lipids in Methanosarcina mazei.

H. Hemmi, Koh-ichi Emi, Kitty Sompiyachoke, 2019, Biochemical and biophysical research communications.

Quantitative analyses of the behavior of exogenously added bacteria during an acidulocomposting process.

T. Nishino, T. Nakayama, H. Hemmi, 2012, Journal of bioscience and bioengineering.

Cloning, Expression, and Characterization ofcis-Polyprenyl Diphosphate Synthase from the Thermoacidophilic Archaeon Sulfolobus acidocaldarius

T. Nishino, T. Nakayama, H. Hemmi, 2001, Journal of bacteriology.

Microbacterium natoriense sp. nov., a novel D-aminoacylase-producing bacterium isolated from soil in Natori, Japan.

T. Nishino, T. Nakayama, H. Hemmi, 2005, International journal of systematic and evolutionary microbiology.

Deletion and insertion of a 192-residue peptide in the active-site domain of glycosyl hydrolase family-2 beta-galactosidases.

T. Nishino, T. Nakayama, M. Inohara-Ochiai, 2002, Journal of bioscience and bioengineering.

Molecular biological analysis of microflora in a garbage treatment process under thermoacidophilic conditions.

T. Nishino, T. Nakayama, H. Hemmi, 2004, Journal of bioscience and bioengineering.

Fusion-type lycopene beta-cyclase from a thermoacidophilic archaeon Sulfolobus solfataricus.

T. Nishino, T. Nakayama, H. Hemmi, 2003, Biochemical and biophysical research communications.

Urinary l-erythro-β-hydroxyasparagine—a novel serine racemase inhibitor and substrate of the Zn2+-dependent d-serine dehydratase

H. Hemmi, T. Yoshimura, Y. Kitaura, 2021, Bioscience reports.

Catalytic mechanism of type 2 isopentenyl diphosphate:dimethylallyl diphosphate isomerase: verification of a redox role of the flavin cofactor in a reaction with no net redox change.

T. Nishino, T. Nakayama, H. Hemmi, 2004, Biochemical and biophysical research communications.

Simultaneous determination of D-amino acids by the coupling method of D-amino acid oxidase with high-performance liquid chromatography.

H. Hemmi, T. Yoshimura, S. Kato, 2011, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Alicyclobacillus sendaiensis sp. nov., a novel acidophilic, slightly thermophilic species isolated from soil in Sendai, Japan.

T. Nishino, T. Nakayama, H. Hemmi, 2003, International journal of systematic and evolutionary microbiology.

Effects of random mutagenesis in a putative substrate-binding domain of geranylgeranyl diphosphate synthase upon intermediate formation and substrate specificity.

T. Nishino, H. Hemmi, C. Ohto, 1997, Journal of biochemistry.

Bacillus subtilis Spore Coat Protein LipC Is a Phospholipase B

H. Hemmi, T. Yoshimura, S. Kato, 2010, Bioscience, biotechnology, and biochemistry.

Zinc biosorption by a zinc-resistant bacterium, Brevibacterium sp. strain HZM-1

T. Nishino, T. Nakayama, H. Hemmi, 2000, Applied Microbiology and Biotechnology.

Total Synthesis and Structure Confirmation of trans-Anhydromevalonate-5-phosphate, a Key Biosynthetic Intermediate of the Archaeal Mevalonate Pathway.

H. Hemmi, T. Shinada, Y. Yasuno, 2021, Journal of natural products.

A versatile cis-prenyltransferase from Methanosarcina mazei catalyzes both C- and O-prenylations

H. Hemmi, H. Unno, M. Matsumoto, 2021, The Journal of biological chemistry.

Total synthesis of geranylgeranylglyceryl phosphate enantiomers: substrates for characterization of 2,3-O-digeranylgeranylglyceryl phosphate synthase.

G. Prestwich, T. Nishino, H. Hemmi, 2006, Organic letters.

Conversion from Farnesyl Diphosphate Synthase to Geranylgeranyl Diphosphate Synthase by Random Chemical Mutagenesis (*)

T. Koyama, T. Nishino, H. Hemmi, 1996, The Journal of Biological Chemistry.

Overexpression of an archaeal geranylgeranyl diphosphate synthase in Escherichia coli cells.

T. Nishino, H. Hemmi, C. Ohto, 1998, Bioscience, biotechnology, and biochemistry.

Dramatic changes in the substrate specificities of prenyltransferase by a single amino acid substitution

T. Koyama, T. Nishino, H. Hemmi, 2002 .

New Role of Flavin as a General Acid-Base Catalyst with No Redox Function in Type 2 Isopentenyl-diphosphate Isomerase*

T. Nishino, T. Nakayama, M. Kusunoki, 2009, Journal of Biological Chemistry.

Novel sugar phosphotransferase system applicable to the efficient labeling of the compounds synthesized via the non-mevalonate pathway in Escherichia coli.

T. Nishino, T. Nakayama, H. Hemmi, 2002, Journal of bioscience and bioengineering.

A new member of MocR/GabR‐type PLP‐binding regulator of d‐alanyl‐d‐alanine ligase in Brevibacillus brevis

I. Miyahara, H. Hemmi, T. Yoshimura, 2015, The FEBS journal.

Altering the substrate chain-length specificity of an α-glucosidase

T. Nishino, T. Nakayama, H. Hemmi, 2003 .

Site-specific mutagenesis at positions 272 and 273 of the Bacillus sp. SAM1606 α-glucosidase to screen mutants with altered specificity for oligosaccharide production by transglucosylation

T. Nishino, T. Nakayama, M. Okada, 2002 .

Deciphering the molecular basis of the broad substrate specificity of alpha-glucosidase from Bacillus sp. SAM1606.

T. Nishino, T. Nakayama, M. Inohara-Ochiai, 2003, Journal of biochemistry.

D-Serine Metabolism and Its Importance in Development of Dictyostelium discoideum

Tamao Saito, H. Hemmi, T. Yoshimura, 2018, Front. Microbiol..

Enzymatic Assay for D-Aspartic Acid Using D-Aspartate Oxidase and Oxaloacetate Decarboxylase

H. Hemmi, T. Yoshimura, S. Kato, 2012, Bioscience, biotechnology, and biochemistry.

Catalytic mechanism of serine racemase from Dictyostelium discoideum

H. Hemmi, T. Yoshimura, M. Goto, 2012, Amino Acids.

A cis‐prenyltransferase from Methanosarcina acetivorans catalyzes both head‐to‐tail and nonhead‐to‐tail prenyl condensation

H. Hemmi, T. Ogawa, T. Yoshimura, 2016, The FEBS journal.

Utilization of an intermediate of the methylerythritol phosphate pathway, (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate, as the prenyl donor substrate for various prenyltransferases

H. Hemmi, T. Yoshimura, Tomokazu Ito, 2018, Bioscience, biotechnology, and biochemistry.

Effect of mutagenesis at the region upstream from the G(Q/E) motif of three types of geranylgeranyl diphosphate synthase on product chain-length.

T. Nishino, T. Nakayama, H. Hemmi, 2009, Journal of bioscience and bioengineering.

Geranylfarnesyl diphosphate synthase from Methanosarcina mazei: Different role, different evolution.

H. Hemmi, T. Ogawa, T. Yoshimura, 2010, Biochemical and biophysical research communications.

Identification and functional analysis of a new type of Z,E‐mixed prenyl reductase from mycobacteria

H. Hemmi, Yoshitaka Tateishi, A. Nishiyama, 2022, The FEBS journal.

The product chain length determination mechanism of type II geranylgeranyl diphosphate synthase requires subunit interaction

T. Koyama, T. Nishino, T. Nakayama, 2008, The FEBS journal.

Collagenolytic Serine-Carboxyl Proteinase from Alicyclobacillus sendaiensis Strain NTAP-1: Purification, Characterization, Gene Cloning, and Heterologous Expression

T. Nishino, T. Nakayama, H. Hemmi, 2003, Applied and Environmental Microbiology.

Mechanism of Pyridoxine 5′-Phosphate Accumulation in Pyridoxal 5′-Phosphate-Binding Protein Deficiency

H. Hemmi, D. Downs, T. Yoshimura, 2022, Journal of bacteriology.

Inhibition of glycine cleavage system by pyridoxine 5′‐phosphate causes synthetic lethality in glyA yggS and serA yggS in Escherichia coli

H. Hemmi, D. Downs, T. Yoshimura, 2020, Molecular microbiology.

Conserved Pyridoxal 5'-Phosphate-Binding Protein YggS Impacts Amino Acid Metabolism through Pyridoxine 5'-Phosphate in Escherichia coli

H. Hemmi, D. Downs, T. Yoshimura, 2019, Applied and Environmental Microbiology.

Production of Ophthalmic Acid Using Engineered Escherichia coli

H. Hemmi, T. Yoshimura, Tomokazu Ito, 2018, Applied and Environmental Microbiology.

Conserved Pyridoxal Protein That Regulates Ile and Val Metabolism

H. Hemmi, T. Yoshimura, Tomokazu Ito, 2013, Journal of bacteriology.

Physiological role of carbon dioxide in spore germination of Clostridium perfringens S40.

H. Hemmi, T. Yoshimura, S. Kato, 2009, Journal of bioscience and bioengineering.

Reconstruction of the “Archaeal” Mevalonate Pathway from the Methanogenic Archaeon Methanosarcina mazei in Escherichia coli Cells

H. Hemmi, Ryo Yoshida, T. Yoshimura, 2020, Applied and Environmental Microbiology.

Conversion of Mevalonate 3-Kinase into 5-Phosphomevalonate 3-Kinase by Single Amino Acid Mutations

H. Kawaide, H. Hemmi, T. Yoshimura, 2019, Applied and Environmental Microbiology.

Modified mevalonate pathway of the archaeon Aeropyrum pernix proceeds via trans-anhydromevalonate 5-phosphate

H. Kawaide, H. Hemmi, T. Yoshimura, 2018, Proceedings of the National Academy of Sciences.

A Single Amino Acid Mutation Converts (R)-5-Diphosphomevalonate Decarboxylase into a Kinase*

H. Kawaide, H. Hemmi, T. Yoshimura, 2016, The Journal of Biological Chemistry.

(R)-Mevalonate 3-Phosphate Is an Intermediate of the Mevalonate Pathway in Thermoplasma acidophilum*♦

H. Kawaide, H. Hemmi, T. Yoshimura, 2014, The Journal of Biological Chemistry.

Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5'-phosphate-binding protein YggS.

H. Hemmi, T. Yoshimura, Tomokazu Ito, 2016, Journal of bioscience and bioengineering.

Molecular cloning and characterization of a thermostable carboxylesterase from an archaeon, Sulfolobus shibatae DSM5389: non-linear kinetic behavior of a hormone-sensitive lipase family enzyme.

T. Nishino, T. Nakayama, H. Hemmi, 2004, Journal of bioscience and bioengineering.

Enzymatic assay of D-serine using D-serine dehydratase from Saccharomyces cerevisiae.

H. Hemmi, T. Yoshimura, Kei Takahashi, 2007, Analytical biochemistry.

Occurrence of the (2R,3S)-Isomer of 2-Amino-3,4-dihydroxybutanoic Acid in the Mushroom Hypsizygus marmoreus.

H. Hemmi, Y. Asada, T. Yoshimura, 2017, Journal of agricultural and food chemistry.

PEGylated D-serine dehydratase as a D-serine reducing agent.

H. Hemmi, T. Yoshimura, T. Matsuda, 2015, Journal of pharmaceutical and biomedical analysis.

Reaction mechanism of Zn2+-dependent d-serine dehydratase: role of a conserved tyrosine residue interacting with pyridine ring nitrogen of pyridoxal 5'-phosphate.

H. Hemmi, T. Yoshimura, Tomokazu Ito, 2014, Journal of biochemistry.

Metal ion dependency of serine racemase from Dictyostelium discoideum

M. Maki, H. Hemmi, S. Hayashi, 2012, Amino Acids.

Role of zinc ion for catalytic activity in d‐serine dehydratase from Saccharomyces cerevisiae

H. Hemmi, T. Yoshimura, Tomokazu Ito, 2012, The FEBS journal.

A highly sensitive enzymatic assay for d- and total serine detection using d-serine dehydratase from Saccharomyces cerevisiae

H. Hemmi, T. Yoshimura, Tomokazu Ito, 2010 .

Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of microbial community structure in landfill leachate.

T. Nishino, T. Nakayama, H. Hemmi, 2009, Journal of hazardous materials.

A Novel Lipolytic Enzyme, YcsK (LipC), Located in the Spore Coat of Bacillus subtilis, Is Involved in Spore Germination

K. Watabe, R. Kuwana, H. Takamatsu, 2007, Journal of bacteriology.

A highly selective biosynthetic pathway to non-natural C50 carotenoids assembled from moderately selective enzymes

T. Maoka, H. Hemmi, S. Takaichi, 2015, Nature Communications.

Construction of an artificial biosynthetic pathway for hyperextended archaeal membrane lipids in the bacterium Escherichia coli

H. Hemmi, Ryo Yoshida, 2020, Synthetic biology.

Acidulocomposting, an Accelerated Composting Process of Garbage under Thermoacidophilic Conditions for Prolonged Periods

T. Nishino, T. Nakayama, H. Hemmi, 2003 .

Type 2 isopentenyl diphosphate isomerase from a thermoacidophilic archaeon Sulfolobus shibatae.

T. Nishino, T. Nakayama, H. Hemmi, 2004, European journal of biochemistry.

Substrate-Induced Change in the Quaternary Structure of Type 2 Isopentenyl Diphosphate Isomerase from Sulfolobus shibatae

H. Hemmi, T. Yoshimura, S. Goda, 2012, Journal of bacteriology.

Covalent modification of reduced flavin mononucleotide in type-2 isopentenyl diphosphate isomerase by active-site-directed inhibitors

Tohru Yoshimura, Hisashi Hemmi, H. Hemmi, 2011, Proceedings of the National Academy of Sciences.

Identification of enzymes involved in the mevalonate pathway of Flavobacterium johnsoniae.

H. Hemmi, T. Yoshimura, Kento Motoyama, 2017, Biochemical and biophysical research communications.

Role of the aminotransferase domain in Bacillus subtilis GabR, a pyridoxal 5′‐phosphate‐dependent transcriptional regulator

Masaru Goto, Shiro Kato, Tohru Yoshimura, 2015, Molecular microbiology.

A Conserved Pyridoxal Protein that Regulates Ile and Val Metabolism 1 2

H. Hemmi, T. Yoshimura, Tomokazu Ito, 2013 .

A phytoene desaturase homolog gene from the methanogenic archaeon Methanosarcina acetivorans is responsible for hydroxyarchaeol biosynthesis.

H. Hemmi, T. Ogawa, T. Yoshimura, 2015, Biochemical and biophysical research communications.

d‐amino acid auxotrophic Escherichia coli strain for in vivo functional cloning of novel d‐amino acid synthetic enzyme

H. Hemmi, T. Yoshimura, Tomokazu Ito, 2023, The FEBS journal.

3 Flavoenzymes involved in non-redox reactions

H. Hemmi, 2012 .

Lysine racemase from a lactic acid bacterium, Oenococcus oeni: structural basis of substrate specificity.

H. Hemmi, T. Yoshimura, S. Kato, 2012, Journal of biochemistry.

Identification and characterization of a serine racemase in the silkworm Bombyx mori.

M. Sumitani, H. Sezutsu, H. Hemmi, 2022, Journal of biochemistry.

Catalytic mechanism of serine racemase from Dictyostelium discoideum

H. Hemmi, S. Hayashi, T. Yoshimura, 2013, Amino Acids.

Biosynthetic machinery for C25,C25-diether archaeal lipids from the hyperthermophilic archaeon Aeropyrum pernix.

H. Hemmi, Ryo Yoshida, T. Yoshimura, 2018, Biochemical and biophysical research communications.

Connected cavity structure enables prenyl elongation across the dimer interface in mutated geranylfarnesyl diphosphate synthase from Methanosarcina mazei.

H. Hemmi, T. Ogawa, T. Yoshimura, 2011, Biochemical and biophysical research communications.

A novel zinc-dependent D-serine dehydratase from Saccharomyces cerevisiae.

H. Hemmi, Y. Mukai, K. Kataoka, 2008, The Biochemical journal.

Recognition of allylic substrates in Sulfolobus acidocaldarius geranylgeranyl diphosphate synthase: analysis using mutated enzymes and artificial allylic substrates.

T. Koyama, T. Nishino, H. Hemmi, 1998, Journal of biochemistry.

Biochemical evidence supporting the presence of the classical mevalonate pathway in the thermoacidophilic archaeon Sulfolobus solfataricus.

H. Hemmi, T. Yoshimura, M. Miyagawa, 2013, Journal of biochemistry.

A [4Fe-4S] cluster resides at the active center of phosphomevalonate dehydratase, a key enzyme in the archaeal modified mevalonate pathway

H. Hemmi, T. Shinada, Tomokazu Ito, 2023, Frontiers in Microbiology.

In Vivo Formation of the Protein Disulfide Bond That Enhances the Thermostability of Diphosphomevalonate Decarboxylase, an Intracellular Enzyme from the Hyperthermophilic Archaeon Sulfolobus solfataricus

H. Hemmi, T. Yoshimura, S. Goda, 2015, Journal of bacteriology.

diphosphomevalonate decarboxylase, an intracellular enzyme from the hyperthermophilic

H. Hemmi, T. Yoshimura, S. Goda, 2015 .

Evolution and properties of alanine racemase from Synechocystis sp. PCC6803.

K. Inagaki, H. Hemmi, T. Yoshimura, 2021, Journal of biochemistry.

Connected cavity structure enables prenyl elongation across the dimer interface in mutated geranylfarnesyl diphosphate synthase from Methanosarcina mazei.

H. Hemmi, T. Ogawa, T. Yoshimura, 2011, Biochemical and biophysical research communications.

Menaquinone-Specific Prenyl Reductase from the Hyperthermophilic Archaeon Archaeoglobus fulgidus

T. Nishino, T. Nakayama, H. Hemmi, 2005, Journal of bacteriology.

A novel geranylgeranyl reductase from the methanogenic archaeon Methanosarcina acetivorans displays unique regiospecificity

H. Hemmi, T. Ogawa, T. Yoshimura, 2014, The FEBS journal.

Effects of producing high levels of hyperthermophile-specific C25,C25-archaeal membrane lipids in Escherichia coli

H. Hemmi, Ryo Yoshida, Tomokazu Ito, 2024, Biochemical and Biophysical Research Communications.