C. Fontes

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Molecular organization and protein stability of the Clostridium thermocellum glucuronoxylan endo-β-1,4-xylanase of family 30 glycoside hydrolase in solution.

A. Goyal, Kedar Sharma, C. Fontes, 2019, Journal of structural biology.

Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates.

Harry J. Gilbert, H. Gilbert, C. Fontes, 2010, Annual review of biochemistry.

The structure of the feruloyl esterase module of xylanase 10B from Clostridium thermocellum provides insights into substrate recognition.

G J Davies, G. Davies, C. Fontes, 2001, Structure.

Discovery of hyperstable carbohydrate‐active enzymes through metagenomics of extreme environments

B. Henrissat, F. Lauro, V. Lombard, 2019, The FEBS journal.

Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila StCBM64C*

M. Romão, A. Carvalho, C. Fontes, 2017, The Journal of Biological Chemistry.

Evidence for Temporal Regulation of the Two Pseudomonas cellulosa Xylanases Belonging to Glycoside Hydrolase Family 11

H. Gilbert, C. Fontes, L. Ferreira, 2002, Journal of bacteriology.

Cellulosomes: Highly Efficient Cellulolytic Complexes.

C. Fontes, P. Bule, V. Alves, 2021, Sub-cellular biochemistry.

Small angle X-ray scattering based structure, modeling and molecular dynamics analyses of family 43 glycoside hydrolase α-L-arabinofuranosidase from Clostridium thermocellum

A. Goyal, Kedar Sharma, C. Fontes, 2019, Journal of biomolecular structure & dynamics.

Thermostable recombinant β-(1→4)-mannanase from C. thermocellum: biochemical characterization and manno-oligosaccharides production.

Arabinda Ghosh, A. Goyal, C. Fontes, 2013, Journal of agricultural and food chemistry.

The family 6 carbohydrate-binding module (CtCBM6B) of Clostridium thermocellum alpha-L-arabinofuranosidase binds xylans and thermally stabilized by Ca2+ ions

A. Goyal, C. Fontes, A. Luis, 2013 .

Crystal structure of a cellulosomal family 3 carbohydrate esterase from Clostridium thermocellum provides insights into the mechanism of substrate recognition.

R. Lewis, H. Gilbert, C. Fontes, 2008, Journal of molecular biology.

Cellulosome assembly revealed by the crystal structure of the cohesin–dockerin complex

Harry J. Gilbert, H. Gilbert, M. Romão, 2003, Proceedings of the National Academy of Sciences of the United States of America.

The Family 6 Carbohydrate Binding Module CmCBM6-2 Contains Two Ligand-binding Sites with Distinct Specificities*[boxs]

B. Henrissat, D. Bolam, H. Gilbert, 2004, Journal of Biological Chemistry.

Efficient pretreatment for bioethanol production from water hyacinth (eichhornia crassipes) involving naturally isolated and recombinant enzymes and its recovery

Saprativ P. Das, Arabinda Ghosh, D. Das, 2013 .

Bioethanol Production Involving Recombinant C. thermocellum Hydrolytic Hemicellulase and Fermentative Microbes

D. Goyal, Saprativ P. Das, D. Das, 2012, Applied Biochemistry and Biotechnology.

Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction

E. Bayer, H. Gilbert, A. Carvalho, 2018, Scientific Reports.

Single Binding Mode Integration of Hemicellulose-degrading Enzymes via Adaptor Scaffoldins in Ruminococcus flavefaciens Cellulosome*

E. Bayer, H. Gilbert, C. Fontes, 2016, The Journal of Biological Chemistry.

Expression, purification, crystallization and preliminary X-ray analysis of CttA, a putative cellulose-binding protein from Ruminococcus flavefaciens.

C. Fontes, S. Najmudin, L. Ferreira, 2015, Acta crystallographica. Section F, Structural biology communications.

Overexpression, crystallization and preliminary X-ray characterization of Ruminococcus flavefaciens scaffoldin C cohesin in complex with a dockerin from an uncharacterized CBM-containing protein.

E. Bayer, C. Fontes, S. Najmudin, 2014, Acta crystallographica. Section F, Structural biology communications.

The resistance of cellulases and xylanases to proteolytic inactivation

J. Hall, B. Hirst, H. Gilbert, 1995, Applied Microbiology and Biotechnology.

T7 Endonuclease I Mediates Error Correction in Artificial Gene Synthesis

A. Sequeira, R. Vincentelli, C. Fontes, 2016, Molecular Biotechnology.

Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum.

K. Sakka, D. Bolam, H. Gilbert, 2009, The Biochemical journal.

Unravelling Glucan Recognition Systems by Glycome Microarrays Using the Designer Approach and Mass Spectrometry

A. Carvalho, C. Fontes, Guangli Yu, 2015, Molecular & Cellular Proteomics.

A novel Cellvibrio mixtus family 10 xylanase that is both intracellular and expressed under non-inducing conditions.

H. Gilbert, G. Hazlewood, C. Fontes, 2000, Microbiology.

Target highlights from the first post‐PSI CASP experiment (CASP12, May–August 2016)

M. Nardini, K. Fidelis, J. Moult, 2018, Proteins.

Influence of a Mannan Binding Family 32 Carbohydrate Binding Module on the Activity of the Appended Mannanase

K. Sakka, H. Gilbert, C. Fontes, 2012, Applied and Environmental Microbiology.

Role of Pectinolytic Enzymes Identified in Clostridium thermocellum Cellulosome

A. Goyal, C. Fontes, J. Prates, 2015, PloS one.

Flexibility and specificity of the cohesin–dockerin interaction: implications for cellulosome assembly and functionality

A. Carvalho, C. Fontes, S. Najmudin, 2012 .

Molecular architecture and structural transitions of a Clostridium thermocellum mini-cellulosome.

R. Melero, M. Romão, A. Carvalho, 2011, Journal of molecular biology.

Deciphering Ligand Specificity of a Clostridium thermocellum Family 35 Carbohydrate Binding Module (CtCBM35) for Gluco- and Galacto- Substituted Mannans and Its Calcium Induced Stability

Arabinda Ghosh, A. Goyal, C. Fontes, 2013, PloS one.

Generation of a Library of Carbohydrate-Active Enzymes for Plant Biomass Deconstruction

B. Henrissat, R. Vincentelli, C. Fontes, 2022, International journal of molecular sciences.

Molecular basis for the preferential recognition of β1,3‐1,4‐glucans by the family 11 carbohydrate‐binding module from Clostridium thermocellum

A. Carvalho, C. Fontes, E. Cabrita, 2019, The FEBS journal.

The Membrane-Bound α-Glucuronidase from Pseudomonas cellulosa Hydrolyzes 4-O-Methyl-d-Glucuronoxylooligosaccharides but Not 4-O-Methyl-d-Glucuronoxylan

H. Gilbert, C. Fontes, L. Ferreira, 2002, Journal of bacteriology.

Novel modular enzymes encoded by a cellulase gene cluster in Cellvibrio mixtus.

H. Gilbert, A. Goyal, C. Fontes, 2006, FEMS microbiology letters.

Evidence for a general role for non-catalytic thermostabilizing domains in xylanases from thermophilic bacteria.

J. Hall, B. Hirst, H. Gilbert, 1995, The Biochemical journal.

The N-terminal family 22 carbohydrate-binding module of xylanase 10B of Clostridium themocellum is not a thermostabilizing domain.

H. Gilbert, A. Goyal, C. Fontes, 2004, FEMS microbiology letters.

Overexpression, purification and crystallization of the two C-terminal domains of the bifunctional cellulase ctCel9D-Cel44A from Clostridium thermocellum.

M. Romão, C. Fontes, S. Najmudin, 2005, Acta crystallographica. Section F, Structural biology and crystallization communications.

Family 6 carbohydrate-binding modules display multiple beta1,3-linked glucan-specific binding interfaces.

D. Bolam, H. Gilbert, C. Fontes, 2009, FEMS microbiology letters.

Purification, crystallization and preliminary X-ray characterization of the pentamodular arabinoxylanase CtXyl5A from Clostridium thermocellum.

M. Romão, C. Fontes, S. Najmudin, 2011, Acta crystallographica. Section F, Structural biology and crystallization communications.

Purification and crystallographic studies of a putative carbohydrate-binding module from the Ruminococcus flavefaciens FD-1 endoglucanase Cel5A.

H. Santos, C. Fontes, S. Najmudin, 2015, Acta crystallographica. Section F, Structural biology communications.

Family 46 Carbohydrate-binding Modules Contribute to the Enzymatic Hydrolysis of Xyloglucan and β-1,3–1,4-Glucans through Distinct Mechanisms*♦

K. Sakka, H. Gilbert, C. Fontes, 2015, The Journal of Biological Chemistry.

Crystallization and preliminary X-ray diffraction analysis of a trimodular endo-β-1,4-glucanase (Cel5B) from Bacillus halodurans.

K. Sakka, H. Santos, C. Fontes, 2014, Acta crystallographica. Section F, Structural biology communications.

Overexpression, purification, crystallization and preliminary X-ray characterization of the fourth scaffoldin A cohesin from Acetivibrio cellulolyticus in complex with a dockerin from a family 5 glycoside hydrolase.

C. Fontes, S. Najmudin, V. Cardoso, 2014, Acta crystallographica. Section F, Structural biology communications.

Escherichia coli expression, purification, crystallization, and structure determination of bacterial cohesin-dockerin complexes.

H. Gilbert, M. Romão, A. Carvalho, 2012, Methods in enzymology.

Identification of tandemly repeated type VI cellulose-binding domains in an endoglucanase from the aerobic soil bacterium Cellvibrio mixtus

H. Gilbert, G. Hazlewood, C. Fontes, 1998, Applied Microbiology and Biotechnology.

Possible roles for a non-modular, thermostable and proteinase-resistant cellulase from the mesophilic aerobic soil bacterium Cellvibrio mixtus

H. Gilbert, G. Hazlewood, C. Fontes, 1997, Applied Microbiology and Biotechnology.

Structure-function studies can improve binding affinity of cohesin-dockerin interactions for multi-protein assemblies.

E. Bayer, M. Romão, A. Carvalho, 2022, International journal of biological macromolecules.

Molecular determinants of substrate specificity revealed by the structure of Clostridium thermocellum arabinofuranosidase 43A from glycosyl hydrolase family 43 subfamily 16.

A. Goyal, Kedar Sharma, C. Fontes, 2016, Acta crystallographica. Section D, Structural biology.

Crystallization and preliminary crystallographic studies of a novel noncatalytic carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome.

A. Goyal, C. Fontes, S. Najmudin, 2015, Acta crystallographica. Section F, Structural biology communications.

Crystallization and preliminary X-ray crystallographic analysis of a novel α-L-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405.

A. Goyal, C. Fontes, S. Najmudin, 2014, Acta Crystallographica Section F Structural Biology Communications.

Purification, crystallization and preliminary X-ray characterization of the third ScaB cohesin in complex with an ScaA X-dockerin from Acetivibrio cellulolyticus.

C. Fontes, S. Najmudin, L. Ferreira, 2014, Acta crystallographica. Section F, Structural biology communications.

Novel insights into the degradation of β-1,3-glucans by the cellulosome of Clostridium thermocellum revealed by structure and function studies of a family 81 glycoside hydrolase.

A. Carvalho, A. Goyal, Kedar Sharma, 2018, International journal of biological macromolecules.

Biochemical characterization of a recombinant derivative (CtLic26A-Cel5) of a cellulosomal cellulase from Clostridium thermocellum

D. Goyal, A. Goyal, Deepmoni Deka, 2009 .

Expression, purification and crystallization of a novel carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome.

C. Fontes, S. Najmudin, L. Ferreira, 2014, Acta crystallographica. Section F, Structural biology communications.

Overproduction, purification, crystallization and preliminary X-ray characterization of the family 46 carbohydrate-binding module (CBM46) of endo-β-1,4-glucanase B (CelB) from Bacillus halodurans.

K. Sakka, H. Santos, C. Fontes, 2014, Acta crystallographica. Section F, Structural biology communications.

Overexpression, crystallization and preliminary X-ray crystallographic analysis of glucuronoxylan xylanohydrolase (Xyn30A) from Clostridium thermocellum.

H. Santos, F. Freire, M. Romão, 2013, Acta crystallographica. Section F, Structural biology and crystallization communications.

Overproduction, purification, crystallization and preliminary X-ray characterization of the C-terminal family 65 carbohydrate-binding module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens.

H. Gilbert, C. Fontes, S. Najmudin, 2013, Acta crystallographica. Section F, Structural biology and crystallization communications.

Purification, crystallization and preliminary X-ray characterization of the Acetivibrio cellulolyticus type I cohesin ScaC in complex with the ScaB dockerin.

C. Fontes, S. Najmudin, L. Ferreira, 2012, Acta crystallographica. Section F, Structural biology and crystallization communications.

Overproduction, purification, crystallization and preliminary X-ray characterization of a novel carbohydrate-binding module of endoglucanase Cel5A from Eubacterium cellulosolvens.

M. Romão, C. Fontes, S. Najmudin, 2011, Acta crystallographica. Section F, Structural biology and crystallization communications.

Family 42 carbohydrate-binding modules display multiple arabinoxylan-binding interfaces presenting different ligand affinities.

M. Romão, C. Fontes, J. Prates, 2010, Biochimica et biophysica acta.

Bacterial xylanase expression in mammalian cells and transgenic mice.

J. Hall, B. Hirst, H. Gilbert, 1999, Journal of biotechnology.

Co-integration and expression of bacterial and genomic transgenes in the pancreatic and intestinal tissues of transgenic mice.

J. Hall, B. Hirst, H. Gilbert, 1997, Gene.

Highly efficient, processive and multifunctional recombinant endoglucanase RfGH5_4 from Ruminococcus flavefaciens FD-1 v3 for recycling lignocellulosic plant biomasses.

A. Goyal, C. Fontes, N. Ahmed, 2022, International journal of biological macromolecules.

A New Member of Family 11 Polysaccharide Lyase, Rhamnogalacturonan Lyase (CtRGLf) from Clostridium thermocellum

A. Goyal, C. Fontes, J. Prates, 2016, Molecular Biotechnology.

Immobilization of bacterial feruloyl esterase on mesoporous silica particles and enhancement of synthetic activity by hydrophobic-modified surface.

L. Olsson, C. Fontes, Sun-Li Chong, 2019, Bioresource technology.

Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module

Mercedes C. Hernández-Gómez, Maja G. Rydahl, H. Gilbert, 2015, FEBS letters.

Functional and structural characterization of family 6 carbohydrate-binding module (CtCBM6A) of Clostridium thermocellum α-L-arabinofuranosidase

A. Goyal, C. Fontes, A. Luis, 2013, Biochemistry (Moscow).

Clostridium thermocellum Xyn10B carbohydrate-binding module 22-2: the role of conserved amino acids in ligand binding.

P. Simpson, D. Bolam, H. Gilbert, 2001, Biochemistry.

A modular xylanase from mesophilic Cellulomonas fimi contains the same cellulose-binding and thermostabilizing domains as xylanases from thermophilic bacteria.

H. Gilbert, G. Hazlewood, C. Fontes, 1996, FEMS microbiology letters.

The Mechanisms by Which Family 10 Glycoside Hydrolases Bind Decorated Substrates*

Harry J. Gilbert, Johan P. Turkenburg, Tracey M. Gloster, 2004, Journal of Biological Chemistry.

Computational and SAXS-based structure insights of pectin acetyl esterase (CtPae12B) of family 12 carbohydrate esterase from Clostridium thermocellum ATCC 27405

A. Goyal, Kedar Sharma, C. Fontes, 2021, Journal of biomolecular structure & dynamics.

A Novel, Noncatalytic Carbohydrate-binding Module Displays Specificity for Galactose-containing Polysaccharides through Calcium-mediated Oligomerization*

B. Henrissat, P. Coutinho, R. Lewis, 2011, The Journal of Biological Chemistry.

Evidence that family 35 carbohydrate binding modules display conserved specificity but divergent function

S. Firbank, R. Lewis, H. Gilbert, 2009, Proceedings of the National Academy of Sciences.

Common Inhibition of Both β‐Glucosidases and β‐Mannosidases by Isofagomine Lactam Reflects Different Conformational Itineraries for Pyranoside Hydrolysis

H. Gilbert, G. Davies, C. Fontes, 2004 .

Structure and Activity of Two Metal Ion-dependent Acetylxylan Esterases Involved in Plant Cell Wall Degradation Reveals a Close Similarity to Peptidoglycan Deacetylases*

A. Brzozowski, J. Turkenburg, G. Davies, 2006, Journal of Biological Chemistry.

Signature active site architectures illuminate the molecular basis for ligand specificity in family 35 carbohydrate binding module.

B. Henrissat, P. Coutinho, H. Gilbert, 2010, Biochemistry.

The Active Site of a Carbohydrate Esterase Displays Divergent Catalytic and Noncatalytic Binding Functions

E. Dodson, D. Bolam, H. Gilbert, 2009, PLoS biology.

A trimodular family 16 glycoside hydrolase from the cellulosome of Ruminococcus flavefaciens displays highly specific licheninase (EC 3.2.1.73) activity.

A. Goyal, C. Fontes, Abhijeet Thakur, 2021, Microbiology.

Molecular Cloning, Expression and Biochemical Characterization of a Family 5 Glycoside Hydrolase First Endo-Mannanase (RfGH5_7) from Ruminococcus flavefaciens FD-1 v3

A. Goyal, C. Fontes, Abhijeet Thakur, 2019, Molecular Biotechnology.

Xyloglucan Is Recognized by Carbohydrate-binding Modules That Interact with β-Glucan Chains*

D. Bolam, H. Gilbert, M. Romão, 2006, Journal of Biological Chemistry.

Targeted expression of microbial cellulases in transgenic animals

Harry J. Gilbert, Judith Hall, B. Hirst, 1995 .

NMR solution structure and SRP54M predicted interaction of the N-terminal sequence (1-30) of the ovine Doppel protein

I. Martins, C. Fontes, E. Cabrita, 2013, Peptides.

Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli

A. Sequeira, R. Vincentelli, C. Fontes, 2017, Microbial Cell Factories.

C. Fontes, A. Horta, R. Pereira, 2016, Animal genetics.

Prion-like Doppel gene polymorphisms and scrapie susceptibility in Portuguese sheep breeds.

C. Fontes, L. Gama, A. Horta, 2010, Animal genetics.

Doppel gene polymorphisms in Portuguese sheep breeds: insights on ram fertility.

C. Fontes, C. C. Marques, M. Vasques, 2009, Animal reproduction science.

The prion-like protein Doppel enhances ovine spermatozoa fertilizing ability.

C. Fontes, E. Cabrita, F. Dias, 2012, Reproduction in domestic animals = Zuchthygiene.

The X6 "thermostabilizing" domains of xylanases are carbohydrate-binding modules: structure and biochemistry of the Clostridium thermocellum X6b domain.

G J Davies, D. Bolam, H. Gilbert, 2000, Biochemistry.

How Family 26 Glycoside Hydrolases Orchestrate Catalysis on Different Polysaccharides

H. Gilbert, G. Davies, A. Goyal, 2005, Journal of Biological Chemistry.

High-Throughput Production of Oxidized Animal Toxins in Escherichia coli.

A. Sequeira, R. Vincentelli, C. Fontes, 2019, Methods in molecular biology.

A Venomics approach coupled to high-throughput toxin production strategies identifies the first venom-derived melanocortin receptor agonists.

A. Sequeira, E. De Pauw, R. Vincentelli, 2020, Journal of medicinal chemistry.

High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery

A. Sequeira, E. De Pauw, R. Vincentelli, 2017, Microbial Cell Factories.

Insights into the Molecular Determinants of Substrate Specificity in Glycoside Hydrolase Family 5 Revealed by the Crystal Structure and Kinetics of Cellvibrio mixtus Mannosidase 5A*

L. Tailford, H. Gilbert, G. Davies, 2004, Journal of Biological Chemistry.

Crystal Structures of Clostridium thermocellum Xyloglucanase, XGH74A, Reveal the Structural Basis for Xyloglucan Recognition and Degradation*

H. Brumer, G. Davies, Martin J. Baumann, 2006, Journal of Biological Chemistry.

Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus.

K. Nelson, E. Mongodin, L. Tailford, 2006, FEMS microbiology letters.

Structure and Function of an Arabinoxylan-specific Xylanase*

Koushik Mazumder, William S York, Joana L A Brás, 2011, The Journal of Biological Chemistry.

The Location of the Ligand-binding Site of Carbohydrate-binding Modules That Have Evolved from a Common Sequence Is Not Conserved*

B. Henrissat, D. Bolam, H. Gilbert, 2001, The Journal of Biological Chemistry.

Recombinant lichenase fromClostridium thermocellum binds glucomannan but not to lichenan: Analysis by affinity electrophoresis

A. Goyal, C. Fontes, S. Ahmed, 2008, Annals of Microbiology.

Homologous xylanases from Clostridium thermocellum: evidence for bi-functional activity, synergism between xylanase catalytic modules and the presence of xylan-binding domains in enzyme complexes.

H. Gilbert, G. Hazlewood, C. Fontes, 1999, The Biochemical journal.

The Family 11 Carbohydrate-binding Module of Clostridium thermocellum Lic26A-Cel5E Accommodates β-1,4- and β-1,3–1,4-Mixed Linked Glucans at a Single Binding Site*

D. Bolam, H. Gilbert, M. Romão, 2004, Journal of Biological Chemistry.

Carbohydrate Depolymerization by Intricate Cellulosomal Systems.

E. Bayer, J. Stern, C. Fontes, 2017, Methods in molecular biology.

The Fine Structure of the Cellulosome Defines the Intricacies of Carbohydrate Deconstruction in the Mammalian Gut

C. Fontes, S. Najmudin, J. Prates, 2020 .

Functional characterization and mutation analysis of family 11, Carbohydrate-Binding Module (CtCBM11) of cellulosomal bifunctional cellulase from Clostridium thermocellum

A. Goyal, C. Fontes, A. Majumder, 2007, Indian Journal of Microbiology.

The Crystal Structure of the Family 6 Carbohydrate Binding Module from Cellvibrio mixtus Endoglucanase 5A in Complex with Oligosaccharides Reveals Two Distinct Binding Sites with Different Ligand Specificities*

B. Henrissat, D. Bolam, H. Gilbert, 2004, Journal of Biological Chemistry.

Mapping Molecular Recognition of β1,3-1,4-Glucans by a Surface Glycan-Binding Protein from the Human Gut Symbiont Bacteroides ovatus

Yan Liu, M. Coimbra, F. Trovão, 2021, Microbiology spectrum.

FliD , the flagellar cap protein from Pseudomonas aeruginosa PAO 1 ( CASP : T 0886 , Ts 886 , PDB : 5 FHY ) : Provided by

M. Nardini, K. Fidelis, J. Moult, 2017 .

Evidence for a dual binding mode of dockerin modules to cohesins

Edward A Bayer, Ana Luísa Carvalho, E. Bayer, 2007, Proceedings of the National Academy of Sciences.

Insights into the structural determinants of cohesin-dockerin specificity revealed by the crystal structure of the type II cohesin from Clostridium thermocellum SdbA.

H. Gilbert, M. Romão, A. Carvalho, 2005, Journal of molecular biology.

Solution structure, dynamics and binding studies of a family 11 carbohydrate-binding module from Clostridium thermocellum (CtCBM11).

F. Freire, M. Romão, A. Carvalho, 2013, The Biochemical journal.

Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-beta-D-xylanase 10B in complex with xylohexaose.

M. Romão, C. Fontes, S. Najmudin, 2008, Acta crystallographica. Section F, Structural biology and crystallization communications.

The family 6 Carbohydrate Binding Module (CtCBM6) of glucuronoxylanase (CtXynGH30) of Clostridium thermocellum binds decorated and undecorated xylans through cleft A.

M. N. Gupta, A. Goyal, C. Fontes, 2015, Archives of biochemistry and biophysics.

Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum.

L. Domingues, C. Fontes, M. Gama, 2008, Protein expression and purification.

Molecular determinants of ligand specificity in family 11 carbohydrate binding modules – an NMR, X‐ray crystallography and computational chemistry approach

Pedro Alexandrino Fernandes, Maria João Ramos, Nuno M F S A Cerqueira, 2008, The FEBS journal.

Putting an N-terminal end to the Clostridium thermocellum xylanase Xyn10B story: crystal structure of the CBM22-1-GH10 modules complexed with xylohexaose.

Shabir Najmudin, H. Gilbert, M. Romão, 2010, Journal of structural biology.

A Novel α-L-Arabinofuranosidase of Family 43 Glycoside Hydrolase (Ct43Araf) from Clostridium thermocellum

M. N. Gupta, Arabinda Ghosh, A. Goyal, 2013, PloS one.

Conservation in the mechanism of glucuronoxylan hydrolysis revealed by the structure of glucuronoxylan xylanohydrolase (CtXyn30A) from Clostridium thermocellum.

F. Freire, A. Goyal, C. Fontes, 2016, Acta crystallographica. Section D, Structural biology.

Structural and biochemical properties of lichenase from Clostridium thermocellum

A. Goyal, C. Fontes, A. Majumder, 2009, Indian Journal of Microbiology.

Understanding How Noncatalytic Carbohydrate Binding Modules Can Display Specificity for Xyloglucan

H. Gilbert, C. Fontes, S. Najmudin, 2012, The Journal of Biological Chemistry.

Cellulosome assembly: paradigms are meant to be broken!

Pedro Bule, Virgínia Mr Pires, Carlos Mga Fontes, 2018, Current opinion in structural biology.

Single versus dual-binding conformations in cellulosomal cohesin-dockerin complexes.

Michael A Nash, Edward A Bayer, E. Bayer, 2016, Current opinion in structural biology.

Expression in Escherichia coli and Purification of Microbial Enzymes for the Production of Enzymatic Analytical Kits to Measure Relevant Metabolites

C. Fontes, J. Prates, L. Ferreira, 2015 .

Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family-related protein-protein interactions

Raphael Lamed, Edward A. Bayer, Pedro Bule, 2017, Scientific Reports.

Elaborate cellulosome architecture of Acetivibrio cellulolyticus revealed by selective screening of cohesin–dockerin interactions

Daniel B. Fried, E. Bayer, R. Lamed, 2014, PeerJ.

Structure–function analyses generate novel specificities to assemble the components of multienzyme bacterial cellulosome complexes

E. Bayer, H. Gilbert, C. Fontes, 2018, The Journal of Biological Chemistry.

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes

E. Bayer, H. Gilbert, A. Carvalho, 2017, Scientific Reports.

Diverse specificity of cellulosome attachment to the bacterial cell surface

E. Bayer, H. Gilbert, M. Romão, 2016, Scientific Reports.

Combined Crystal Structure of a Type I Cohesin

E. Bayer, S. Fleishman, H. Gilbert, 2015, The Journal of Biological Chemistry.

Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition

Maja G. Rydahl, B. Henrissat, P. Coutinho, 2016, Proceedings of the National Academy of Sciences.

High-Throughput Production of a New Library of Human Single and Tandem PDZ Domains Allows Quantitative PDZ-Peptide Interaction Screening Through High-Throughput Holdup Assay.

A. Sequeira, R. Vincentelli, Y. Nominé, 2019, Methods in molecular biology.

A Novel Platform for High-Throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli.

Renaud Vincentelli, Ana Filipa Sequeira, Joana L A Brás, 2017, Methods in molecular biology.

Structural and functional insights into the glycoside hydrolase family 30 xylanase of the rumen bacterium Ruminococcus flavefaciens

A. Goyal, C. Fontes, Shubha Singh, 2022, Journal of Molecular Structure.

A dual cohesin–dockerin complex binding mode in Bacteroides cellulosolvens contributes to the size and complexity of its cellulosome

A. Carvalho, C. Fontes, S. Najmudin, 2021, The Journal of biological chemistry.

Molecular determinants of substrate specificity in the feruloyl esterase module of xylanase 10B from Clostridium thermocellum.

G. Davies, C. Fontes, J. Prates, 2005, Acta crystallographica. Section D, Biological crystallography.

Directed evolution of the type C feruloyl esterase from Fusarium oxysporum FoFaeC and molecular docking analysis of its improved variants.

S. Bozonnet, C. Fontes, U. Rova, 2019, New biotechnology.

Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis

E. Bayer, H. Gilbert, M. Romão, 2011, Proceedings of the National Academy of Sciences.

The Mechanism by Which Arabinoxylanases Can Recognize Highly Decorated Xylans

B. Henrissat, H. Gilbert, C. Fontes, 2016, The Journal of Biological Chemistry.

Evolution of the feruloyl esterase MtFae1a from Myceliophthora thermophila towards improved catalysts for antioxidants synthesis

C. Fontes, U. Rova, P. Christakopoulos, 2018, Applied Microbiology and Biotechnology.

Small Angle X-ray Scattering Analysis of Clostridium thermocellum Cellulosome N-terminal Complexes Reveals a Highly Dynamic Structure*

E. Bayer, Z. Jia, M. Currie, 2013, The Journal of Biological Chemistry.

Cell-surface Attachment of Bacterial Multienzyme Complexes Involves Highly Dynamic Protein-Protein Anchors*

Pedro Bule, Victor D Alves, Edward A Bayer, 2015, The Journal of Biological Chemistry.

Novel Clostridium thermocellum Type I Cohesin-Dockerin Complexes Reveal a Single Binding Mode*

Harry J. Gilbert, Victor D. Alves, Shabir Najmudin, 2012, The Journal of Biological Chemistry.

Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction

E. Bayer, H. Gilbert, A. Carvalho, 2018, Scientific Reports.

Probing the beta-1,3:1,4 glucanase, CtLic26A, with a thio-oligosaccharide and enzyme variants.

H. Gilbert, G. Davies, C. Fontes, 2008, Organic & biomolecular chemistry.

NMR solution structure and SRP54M predicted interaction of the N-terminal sequence (1-30) of the ovine Doppel protein

I. Martins, C. Fontes, E. Cabrita, 2013, Peptides.

Single Binding Mode Cohesin-Dockerin Complexes Reveal a Type I Clostridium thermocellum Novel Protein Structure and Folding :

D. Bolam, H. Gilbert, M. Romão, 2012 .

Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins

A. Sequeira, R. Vincentelli, C. Fontes, 2016, BMC Biotechnology.

A Novel Platform for High-Throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli.

A. Sequeira, R. Vincentelli, C. Fontes, 2017, Methods in molecular biology.

Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum.

K. Sakka, D. Bolam, H. Gilbert, 2009, The Biochemical journal.

Family 46 Carbohydrate-binding Modules Contribute to the Enzymatic Hydrolysis of Xyloglucan and (cid:2) -1,3–1,4-Glucans through Distinct Mechanisms

K. Sakka, C. Fontes, S. Najmudin, 2020 .

Mannan specific family 35 carbohydrate-binding module (CtCBM35) of Clostridium thermocellum: structure analysis and ligand binding

Arabinda Ghosh, A. Goyal, C. Fontes, 2014, Biologia.