S. Lesley

发表

Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline

Adam Godzik, Chittibabu Guda, Peter G Schultz, 2002, Proceedings of the National Academy of Sciences of the United States of America.

Size and shape of detergent micelles determined by small-angle X-ray scattering.

S. Doniach, J. Lipfert, S. Lesley, 2007, The journal of physical chemistry. B.

Structures of a bifunctional cell wall hydrolase CwlT containing a novel bacterial lysozyme and an NlpC/P60 DL-endopeptidase.

Mitchell D. Miller, A. Godzik, A. Deacon, 2014, Journal of molecular biology.

Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts

Eric Koesema, S. Lesley, H. Klock, 2008, Proteins.

Structural basis of murein peptide specificity of a gamma-D-glutamyl-l-diamino acid endopeptidase.

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2009, Structure.

Structural Biology and Crystallization Communications the Jcsg High-throughput Structural Biology Pipeline

Kurt Wüthrich, Adam Godzik, Ashley M. Deacon, 2022 .

A scaleable and integrated crystallization pipeline applied to mining the Thermotoga maritima proteome

A. Deacon, S. Lesley, H. Klock, 2004, Journal of Structural and Functional Genomics.

Screening the mammalian extracellular proteome for regulators of embryonic human stem cell pluripotency

A. Orth, A. Su, P. Schultz, 2010, Proceedings of the National Academy of Sciences.

On the use of DXMS to produce more crystallizable proteins: Structures of the T. maritima proteins TM0160 and TM1171

Virgil L. Woods, I. Wilson, G. Spraggon, 2004, Protein science : a publication of the Protein Society.

Rapid refinement of crystallographic protein construct definition employing enhanced hydrogen/deuterium exchange MS.

Virgil L. Woods, R. Stevens, I. Wilson, 2004, Proceedings of the National Academy of Sciences of the United States of America.

Protein production and purification

K. Gunsalus, G. Montelione, J. Bray, 2008, Nature Methods.

Commensal microbiota is hepatoprotective and prevents liver fibrosis in mice

D. Brenner, L. Eckmann, S. Lesley, 2015, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

Crystal structure of TM1367 from Thermotoga maritima at 1.90 Å resolution reveals an atypical member of the cyclophilin (peptidylprolyl isomerase) fold

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2006, Proteins.

Crystal Structure of a Two-Subunit TrkA Octameric Gating Ring Assembly

Mitchell D. Miller, I. Wilson, G. Spraggon, 2015, PloS one.

Three-Dimensional Structural View of the Central Metabolic Network of Thermotoga maritima

Ines Thiele, Adam Godzik, Ashley M Deacon, 2009, Science.

Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells

Mitchell D. Miller, A. Godzik, A. Deacon, 2015, Proceedings of the National Academy of Sciences.

Cofactor‐induced reversible folding of Flavodoxin‐4 from Lactobacillus acidophilus

Kurt Wüthrich, Adam Godzik, Ashley M Deacon, 2015, Protein science : a publication of the Protein Society.

Crystal structure of a putative quorum sensing‐regulated protein (PA3611) from the Pseudomonas‐specific DUF4146 family

Mitchell D. Miller, A. Godzik, A. Deacon, 2014, Proteins.

Structural analysis of arabinose-5-phosphate isomerase from Bacteroides fragilis and functional implications.

Mitchell D. Miller, A. Godzik, A. Deacon, 2014, Acta crystallographica. Section D, Biological crystallography.

Structure and Function of the DUF2233 Domain in Bacteria and in the Human Mannose 6-Phosphate Uncovering Enzyme*

Adam Godzik, Hsiu-Ju Chiu, Ashley M Deacon, 2013, The Journal of Biological Chemistry.

Functional and structural characterization of a thermostable acetyl esterase from Thermotoga maritima

Mitchell D. Miller, A. Deacon, L. T. Ten Eyck, 2012, Proteins.

Identification of coumarin derivatives as a novel class of allosteric MEK1 inhibitors.

Yi Liu, Nathanael Gray, Scott A Lesley, 2005, Bioorganic & medicinal chemistry letters.

Selecting folded proteins from a library of secondary structural elements.

Peter G Schultz, Roshan Perera, Scott A Lesley, 2008, Journal of the American Chemical Society.

Expression, purification, and characterization of Thermotoga maritima membrane proteins for structure determination

Sebastian Doniach, Linda Columbus, Scott A Lesley, 2006, Protein science : a publication of the Protein Society.

Crystal structure of an α/β serine hydrolase (YDR428C) from Saccharomyces cerevisiae at 1.85 Å resolution

Adam Godzik, Henry van den Bedem, Slawomir K. Grzechnik, 2004 .

Shotgun Crystallization Strategy for Structural Genomics II: Crystallization Conditions that Produce High Resolution Structures for T. maritima Proteins

A. Deacon, R. Stevens, R. Page, 2005, Journal of Structural and Functional Genomics.

A neural net approach to the automated analysis of crystallization trials

J. Priestle, G. Spraggon, S. Lesley, 2002 .

Crystal structure of histidine phosphotransfer protein ShpA, an essential regulator of stalk biogenesis in Caulobacter crescentus.

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2009, Journal of molecular biology.

A Distinct Type of Pilus from the Human Microbiome

A. Godzik, A. Deacon, I. Wilson, 2016, Cell.

Mechanistic studies of a flavin-dependent thymidylate synthase.

Peter Kuhn, Scott A Lesley, Amnon Kohen, 2004, Biochemistry.

Folate binding site of flavin-dependent thymidylate synthase

S. Lesley, I. Mathews, Arjun Prabhakar, 2012, Proceedings of the National Academy of Sciences.

Trapping of an intermediate in the reaction catalyzed by flavin-dependent thymidylate synthase.

S. Lesley, B. Palfey, T. V. Mishanina, 2012, Journal of the American Chemical Society.

The relationships between oxidase and synthase activities of flavin dependent thymidylate synthase (FDTS).

S. Lesley, E. Koehn, A. Kohen, 2007, Chemical communications.

Crystal structure of thy1, a thymidylate synthase complementing protein from Thermotoga maritima at 2.25 Å resolution

Adam Godzik, Henry van den Bedem, Slawomir K. Grzechnik, 2002, Proteins.

Efficient Preparation of Site-Specific Antibody-Drug Conjugates Using Phosphopantetheinyl Transferases.

Scott A Lesley, Badry Bursulaya, Susan E. Cellitti, 2015, Bioconjugate chemistry.

Crystal structure of a PIN (PilT N‐terminus) domain (AF0591) from Archaeoglobus fulgidus at 1.90 Å resolution

Adam Godzik, Henry van den Bedem, Slawomir K. Grzechnik, 2004, Proteins.

Crystal structure of an iron‐containing 1,3‐propanediol dehydrogenase (TM0920) from Thermotoga maritima at 1.3 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2003, Proteins.

D-Ornithine coopts pyrrolysine biosynthesis to make and insert pyrroline-carboxy-lysine.

Susan E. Cellitti, A. Brock, S. Lesley, 2011, Nature chemical biology.

UHM-ULM interactions in the RBM39-U2AF65 splicing-factor complex.

A. Godzik, A. Deacon, K. Wüthrich, 2016, Acta crystallographica. Section D, Structural biology.

Comparative structural analysis of a novel glutathioneS‐transferase (ATU5508) from Agrobacterium tumefaciens at 2.0 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2006, Proteins.

Site-Directed Mutagenesis Using Altered /gb-Lactamase Specificity

S. Lesley, C. Andrews, 2002 .

Bacteria on steroids: the enzymatic mechanism of an NADH-dependent dehydrogenase that regulates the conversion of cortisol to androgen in the gut microbiome

Rafael C. Bernardi, Marcelo C. R. Melo, Z. Luthey-Schulten, 2020, bioRxiv.

Crystal structure of an Apo mRNA decapping enzyme (DcpS) from Mouse at 1.83 Å resolution

Adam Godzik, Henry van den Bedem, Slawomir K. Grzechnik, 2005, Proteins.

Insights into substrate specificity of geranylgeranyl reductases revealed by the structure of digeranylgeranylglycerophospholipid reductase, an essential enzyme in the biosynthesis of archaeal membrane lipids.

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Journal of molecular biology.

Cloning and in vivo and in vitro regulation of cyclic AMP-dependent carbon starvation genes from Escherichia coli

R. Burgess, P. Blum, S. Lesley, 1990, Journal of bacteriology.

Synthetic symmetrization in the crystallization and structure determination of CelA from Thermotoga maritima

T. Yeates, M. Sawaya, D. R. Banatao, 2010, Protein science : a publication of the Protein Society.

Crystal structure of a novel Sm‐like protein of putative cyanophage origin at 2.60 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, K. D. Murphy, 2009, Proteins.

Bacterial Pleckstrin Homology Domains: A Prokaryotic Origin for the PH Domain

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Journal of molecular biology.

Crystal structure of a transcription regulator (TM1602) from Thermotoga maritima at 2.3 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2007, Proteins.

Crystal structure of TM1030 from Thermotoga maritima at 2.3 Å resolution reveals molecular details of its transcription repressor function

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2007, Proteins: Structure, Function, and Bioinformatics.

Crystal structure of MtnX phosphatase from Bacillus subtilis at 2.0 Å resolution provides a structural basis for bipartite phosphomonoester hydrolysis of 2‐hydroxy‐3‐keto‐5‐methylthiopentenyl‐1‐phosphate

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2007, Proteins.

Crystal structure of an ORFan protein (TM1622) from Thermotoga maritima at 1.75 Å resolution reveals a fold similar to the Ran‐binding protein Mog1p

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2006, Proteins.

Crystal structure of the ApbE protein (TM1553) from Thermotoga maritima at 1.58 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2006, Proteins.

Crystal structure of AICAR transformylase IMP cyclohydrolase (TM1249) from Thermotoga maritima at 1.88 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2008, Proteins.

Structure of a Novel Winged-Helix Like Domain from Human NFRKB Protein

A. Godzik, A. Deacon, R. Fletterick, 2012, PloS one.

Molecular characterization of novel pyridoxal‐5′‐phosphate‐dependent enzymes from the human microbiome

Mitchell D. Miller, A. Godzik, A. Deacon, 2014, Protein science : a publication of the Protein Society.

Structures of single‐layer β‐sheet proteins evolved from β‐hairpin repeats

A. Godzik, A. Deacon, I. Wilson, 2019, Protein science : a publication of the Protein Society.

A Fully Automated High-Throughput Flow Cytometry Screening System Enabling Phenotypic Drug Discovery

Chris Shaw, Yingyao Zhou, Jennifer Harris, 2018, SLAS discovery : advancing life sciences R & D.

Crystal structures of human HSP90α-complexed with dihydroxyphenylpyrazoles

J. Caldwell, S. Lesley, A. Kreusch, 2005 .

Genome pool strategy for structural coverage of protein families.

A. Godzik, A. Deacon, I. Wilson, 2008, Structure.

Structural and Sequence Analysis of Imelysin-Like Proteins Implicated in Bacterial Iron Uptake

Mitchell D. Miller, A. Godzik, A. Deacon, 2011, PloS one.

Structures of the first representatives of Pfam family PF06684 (DUF1185) reveal a novel variant of the Bacillus chorismate mutase fold and suggest a role in amino-acid metabolism

Slawomir K. Grzechnik, Mitchell D. Miller, K. D. Murphy, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

The crystal structure of a bacterial Sufu-like protein defines a novel group of bacterial proteins that are similar to the N-terminal domain of human Sufu

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Protein science : a publication of the Protein Society.

Structure of the first representative of Pfam family PF04016 (DUF364) reveals enolase and Rossmann-like folds that combine to form a unique active site with a possible role in heavy-metal chelation

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Structural Biology and Crystallization Communications Structures of the First Representatives of Pfam Family Pf06938 (duf1285) Reveal a New Fold with Repeated Structural Motifs and Possible Involvement in Signal Transduction

Mitchell D. Miller, A. Godzik, A. Deacon, 2022 .

Structure of Thermotoga maritima TM0439: implications for the mechanism of bacterial GntR transcription regulators with Zn2+-binding FCD domains.

I. Wilson, D. Giedroc, L. Hung, 2009, Acta crystallographica. Section D, Biological crystallography.

Crystal structure of an alanine‐glyoxylate aminotransferase from Anabaena sp. at 1.70 Å resolution reveals a noncovalently linked PLP cofactor

Adam Godzik, Henry van den Bedem, Slawomir K. Grzechnik, 2005, Proteins.

Crystal structure of an ADP‐ribosylated protein with a cytidine deaminase‐like fold, but unknown function (TM1506), from Thermotoga maritima at 2.70 Å resolution

Adam Godzik, Henry van den Bedem, Slawomir K. Grzechnik, 2008, Proteins.

Crystal structure of a metal‐dependent phosphoesterase (YP_910028.1) from Bifidobacterium adolescentis: Computational prediction and experimental validation of phosphoesterase activity

Adam Godzik, Mary Jo Ondrechen, Hsiu-Ju Chiu, 2011, Proteins.

Crystal structure of the first eubacterial Mre11 nuclease reveals novel features that may discriminate substrates during DNA repair.

John A Tainer, Adam Godzik, Henry van den Bedem, 2010, Journal of molecular biology.

Crystal structure of a zinc‐containing glycerol dehydrogenase (TM0423) from Thermotoga maritima at 1.5 Å resolution

Adam Godzik, Slawomir K. Grzechnik, Chittibabu Guda, 2002, Proteins.

Structure of the pilus assembly protein TadZ from Eubacterium rectale: implications for polar localization

Mitchell D. Miller, A. Godzik, A. Deacon, 2012, Molecular microbiology.

Secreted PCSK9 promotes LDL receptor degradation independently of proteolytic activity.

Jennifer L. Harris, J. Trauger, G. Spraggon, 2007, The Biochemical journal.

Structural and functional characterization of BaiA, an enzyme involved in secondary bile acid synthesis in human gut microbe

S. Lesley, H. Axelrod, H. Chiu, 2014, Proteins.

Nanoscale Mapping of EGFR and c-MET Protein Environments on Lung Cancer Cell Surfaces via Therapeutic Antibody Photocatalyst Conjugates.

R. Oslund, S. Lesley, A. Olow, 2022, ACS chemical biology.

Structure of an MmyB-Like Regulator from C. aurantiacus, Member of a New Transcription Factor Family Linked to Antibiotic Metabolism in Actinomycetes

Mitchell D. Miller, A. Godzik, A. Deacon, 2012, PloS one.

Insights into substrate specificity of geranylgeranyl reductases revealed by the structure of digeranylgeranylglycerophospholipid reductase from Thermoplasma acidophilum , an essential enzyme in the biosynthesis of archaeal membrane lipids

Mitchell D. Miller, Deacon, A. Godzik, 2011 .

The structure of BVU2987 from Bacteroides vulgatus reveals a superfamily of bacterial periplasmic proteins with possible inhibitory function

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Crystal structure of a novel archaeal AAA+ ATPase SSO1545 from Sulfolobus solfataricus

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2009, Proteins.

Crystal structure of phosphoribosylformyl‐glycinamidine synthase II, PurS subunit (TM1244) from Thermotoga maritima at 1.90 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2006, Proteins.

A Human IgSF Cell-Surface Interactome Reveals a Complex Network of Protein-Protein Interactions

Paul W Anderson, Woj M. Wojtowicz, K. Garcia, 2020, Cell.

Supramolecular Organization of the Repetitive Backbone Unit of the Streptococcus pneumoniae Pilus

R. Rappuoli, Eric Koesema, V. Masignani, 2010, PloS one.

Preparation and use of E. coli S-30 extracts.

S. Lesley, 1995, Methods in molecular biology.

Methods and Results for Semi-automated Cloning Using Integrated Robotics

Eric Koesema, S. Lesley, H. Klock, 2005, Journal of Structural and Functional Genomics.

The Joint Center for Structural Genomics

A. Godzik, A. Deacon, K. Hodgson, 2011 .

High-throughput Protein Production for X-ray Crystallography and Use of Size Exclusion Chromatography to Validate or Refute Computational Biological Unit Predictions

S. Lesley, H. Klock, D. Mcmullan, 2005, Journal of Structural and Functional Genomics.

Characterization and optimization of selective, nonpeptidic inhibitors of cathepsin S with an unprecedented binding mode.

G. Spraggon, S. Lesley, J. Ellman, 2007, Journal of medicinal chemistry.

Simple affinity purification of antibodies using in vivo biotinylation of a fusion protein.

S. Lesley, D. Groskreutz, 1997, Journal of immunological methods.

The Polymerase Incomplete Primer Extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis.

Scott A Lesley, Heath E Klock, S. Lesley, 2009, Methods in molecular biology.

Use of in vitro protein synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies.

R. Burgess, S. Lesley, M. Brow, 1991, The Journal of biological chemistry.

Site-specific protein modifications through pyrroline-carboxy-lysine residues

Susan E. Cellitti, A. Brock, S. Lesley, 2011, Proceedings of the National Academy of Sciences.

Structure of the first representative of Pfam family PF09410 (DUF2006) reveals a structural signature of the calycin superfamily that suggests a role in lipid metabolism

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2009, Acta crystallographica. Section F, Structural biology and crystallization communications.

Solubilization of native integral membrane proteins in aqueous buffer by noncovalent chelation with monomethoxy poly(ethylene glycol) (mPEG) polymers.

A. Brock, S. Lesley, Linda Okach, 2011, Bioconjugate chemistry.

Metabolomics analysis reveals large effects of gut microflora on mammalian blood metabolites

W. R. Wikoff, P. Schultz, G. Siuzdak, 2009, Proceedings of the National Academy of Sciences.

Structural Analysis of Papain-Like NlpC/P60 Superfamily Enzymes with a Circularly Permuted Topology Reveals Potential Lipid Binding Sites

Mitchell D. Miller, A. Godzik, A. Deacon, 2011, PloS one.

Identification and structural characterization of heme binding in a novel dye‐decolorizing peroxidase, TyrA

Slawomir K. Grzechnik, Mitchell D. Miller, K. D. Murphy, 2007, Proteins.

Structural Insights into the Catalytic Mechanism of Bacterial Guanosine-diphospho-d-mannose Pyrophosphorylase and Its Regulation by Divalent Ions*

P. Kuhn, M. Pelissier, S. Lesley, 2010, The Journal of Biological Chemistry.

Open and closed conformations of two SpoIIAA-like proteins (YP_749275.1 and YP_001095227.1) provide insights into membrane association and ligand binding

Mitchell D. Miller, A. Godzik, A. Deacon, 2009, Acta crystallographica. Section F, Structural biology and crystallization communications.

The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2009, Acta crystallographica. Section F, Structural biology and crystallization communications.

Structure of a virulence regulatory factor CvfB reveals a novel winged helix RNA binding module.

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Structure.

Structure of LP2179, the first representative of Pfam family PF08866, suggests a new fold with a role in amino-acid metabolism

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2009, Acta crystallographica. Section F, Structural biology and crystallization communications.

High-throughput proteomics

Scott A. Lesley, Marc Nasoff, Andreas Kreusch, 2001, SPIE BiOS.

A genetic approach to the generation of antibodies with enhanced catalytic activities.

P. Schultz, S. Lesley, P. Patten, 1993, Proceedings of the National Academy of Sciences of the United States of America.

Gene expression response to misfolded protein as a screen for soluble recombinant protein.

Scott A Lesley, Heath E Klock, S. Lesley, 2002, Protein engineering.

Co-regulatory networks of human serum proteins link genetics to disease

Xia Yang, Le Shu, Vilmundur Gudnason, 2018, Science.

Characterization of the Escherichia coli transcription factor sigma 70: localization of a region involved in the interaction with core RNA polymerase.

R. Burgess, S. Lesley, Scott A. Lesley, 1989, Biochemistry.

Crystal structure of the ALK (anaplastic lymphoma kinase) catalytic domain.

Glen Spraggon, Scott A Lesley, Nanxin Li, 2010, The Biochemical journal.

Structure and functional characterization of a bile acid 7α dehydratase BaiE in secondary bile acid synthesis

Mitchell D. Miller, I. Wilson, S. Lesley, 2016, Proteins.

Structure and functional characterization of a bile acid 7α dehydratase BaiE in secondary bile acid synthesis

Mitchell D. Miller, I. Wilson, S. Lesley, 2016, Proteins: Structure, Function, and Bioinformatics.

Crystal structure of uronate isomerase (TM0064) from Thermotoga maritima at 2.85 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2003, Proteins.

Combinatorial Use of Antibodies to Secreted Mycobacterial Proteins in a Host Immune System-Independent Test for Tuberculosis

Scott A. Lesley, Maria L. Gennaro, M. Gennaro, 2001, Journal of Clinical Microbiology.

Structural and Functional Characterizations of SsgB, a Conserved Activator of Developmental Cell Division in Morphologically Complex Actinomycetes*

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2009, The Journal of Biological Chemistry.

The structure of Jann_2411 (DUF1470) from Jannaschia sp. at 1.45 Å resolution reveals a new fold (the ABATE domain) and suggests its possible role as a transcription regulator

Slawomir K. Grzechnik, Mitchell D. Miller, K. D. Murphy, 2009, Acta crystallographica. Section F, Structural biology and crystallization communications.

High-throughput proteomics: protein expression and purification in the postgenomic world.

S A Lesley, S. Lesley, 2001, Protein expression and purification.

XtalPred: a web server for prediction of protein crystallizability

Leszek Rychlewski, Adam Godzik, Lukasz Jaroszewski, 2007, Bioinform..

Active site conformational changes of prostasin provide a new mechanism of protease regulation by divalent cations

Aaron Shipway, Jennifer L. Harris, G. Spraggon, 2009, Protein science : a publication of the Protein Society.

A structural basis for the regulatory inactivation of DnaA.

Mitchell D. Miller, A. Godzik, A. Deacon, 2009, Journal of molecular biology.

Supplemental Data Structural Basis of Murein Peptide Specificity of a γ-D-Glutamyl-L-Diamino Acid Endopeptidase

Linda, Slawomir K. Grzechnik, Mitchell D. Miller, 2009 .

An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX Gene

Scott A. Lesley, Irimpan I. Mathews, Amnon Kohen, 2009, Nature.

The Thermotoga maritima Trk Potassium Transporter—from Frameshift to Function

S. Lesley, H. Johnson, E. Hampton, 2009, Journal of bacteriology.

Crystal structure of an orphan protein (TM0875) from Thermotoga maritima at 2.00‐Å resolution reveals a new fold

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins.

NMR for structural proteomics of Thermotoga maritima: Screening and structure determination

Torsten Herrmann, Kurt Wüthrich, Scott A. Lesley, 2004, Journal of Structural and Functional Genomics.

Shotgun crystallization strategy for structural genomics: an optimized two-tiered crystallization screen against the Thermotoga maritima proteome.

Slawomir K. Grzechnik, Rebecca Page, Peter Kuhn, 2003, Acta crystallographica. Section D, Biological crystallography.

Crystal structures of two novel dye‐decolorizing peroxidases reveal a β‐barrel fold with a conserved heme‐binding motif

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2007, Proteins.

Crystal structure of an aspartate aminotransferase (TM1255) from Thermotoga maritima at 1.90 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins.

Identification of a heat shock promoter in the topA gene of Escherichia coli

R. Burgess, S. Lesley, Y. Tse‐Dinh, 1990, Journal of bacteriology.

Crystal Structure of a Divalent Metal Ion Transporter CorA at 2.9 Angstrom Resolution

P. Nordlund, A. Kohl, D. M. Molina, 2006, Science.

Crystal structures of three representatives of a new Pfam family PF14869 (DUF4488) suggest they function in sugar binding/uptake

Mitchell D. Miller, A. Godzik, A. Deacon, 2014, Protein science : a publication of the Protein Society.

Structure of BT_3984, a member of the SusD/RagB family of nutrient-binding molecules

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold

Mitchell D. Miller, A. Godzik, A. Deacon, 2009, Acta crystallographica. Section F, Structural biology and crystallization communications.

The challenge of protein structure determination—lessons from structural genomics

Leszek Rychlewski, Adam Godzik, Ana P. C. Rodrigues, 2007, Protein science : a publication of the Protein Society.

Crystal Structure and Activity Studies of the C11 Cysteine Peptidase from Parabacteroides merdae in the Human Gut Microbiome*

A. Godzik, A. Deacon, I. Wilson, 2016, The Journal of Biological Chemistry.

Insights into Substrate Specificity of NlpC/P60 Cell Wall Hydrolases Containing Bacterial SH3 Domains

A. Godzik, A. Deacon, I. Wilson, 2015, mBio.

Structure-guided functional characterization of DUF1460 reveals a highly specific NlpC/P60 amidase family.

A. Godzik, A. Deacon, I. Wilson, 2014, Structure.

The structure of SSO2064, the first representative of Pfam family PF01796, reveals a novel two-domain zinc-ribbon OB-fold architecture with a potential acyl-CoA-binding role

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Identification of subunits of gonococcal RNA polymerase by immunoblot analysis: evidence for multiple sigma factors

S. Lesley, V. Clark, K. Klimpel, 1989, Journal of bacteriology.

Identification of unknown protein function using metabolite cocktail screening.

Wladek Minor, Marcin Cymborowski, Scott A Lesley, 2012, Structure.

Crystal structure of gamma-glutamyl phosphate reductase (TM0293) from Thermotoga maritima at 2.0 A resolution.

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins.

Crystal structure of γ‐glutamyl phosphate reductase (TM0293) from Thermotoga maritima at 2.0 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2003 .

The self-inhibited structure of full-length PCSK9 at 1.9 Å reveals structural homology with resistin within the C-terminal domain

Jennifer L. Harris, G. Spraggon, S. Lesley, 2007, Proceedings of the National Academy of Sciences.

Structure of the γ-d-glutamyl-l-diamino acid endopeptidase YkfC from Bacillus cereus in complex with l-Ala-γ-d-Glu: insights into substrate recognition by NlpC/P60 cysteine peptidases

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Crystal structure of a single‐stranded DNA‐binding protein (TM0604) from Thermotoga maritima at 2.60 Å resolution

Adam Godzik, Henry van den Bedem, Slawomir K. Grzechnik, 2006, Proteins.

Crystal structure of a member of a novel family of dioxygenases (PF10014) reveals a conserved cupin fold and active site

Mitchell D. Miller, A. Godzik, A. Deacon, 2014, Proteins.

Mixing and matching detergents for membrane protein NMR structure determination.

Adelene Y. L. Sim, S. Doniach, J. Lipfert, 2009, Journal of the American Chemical Society.

Crystal structure of S‐adenosylmethionine:tRNA ribosyltransferase‐isomerase (QueA) from Thermotoga maritima at 2.0 Å resolution reveals a new fold

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2005, Proteins.

Crystal structure of a tandem cystathionine‐β‐synthase (CBS) domain protein (TM0935) from Thermotoga maritima at 1.87 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins.

Crystal structure of a novel Thermotoga maritima enzyme (TM1112) from the cupin family at 1.83 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins: Structure, Function, and Bioinformatics.

Crystal structure of a putative oxalate decarboxylase (TM1287) from Thermotoga maritima at 1.95 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins.

Crystal structure of a methionine aminopeptidase (TM1478) from Thermotoga maritima at 1.9 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins: Structure, Function, and Bioinformatics.

Letter to the Editor: NMR Structure Determination of the Hypothetical Protein TM1290 from Thermotoga Maritima using Automated NOESY Analysis

K. Wüthrich, T. Herrmann, W. Peti, 2004, Journal of biomolecular NMR.

Crystal structure of a ribose‐5‐phosphate isomerase RpiB (TM1080) from Thermotoga maritima at 1.90 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins.

Crystal structure of a phosphoribosylaminoimidazole mutase PurE (TM0446) from Thermotoga maritima at 1.77‐Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins.

Crystal structure of a putative PII‐like signaling protein (TM0021) from Thermotoga maritima at 2.5 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins.

Parallel methods for expression and purification.

S. Lesley, 2009, Methods in enzymology.

Protein Production and Crystallization at the Joint Center for Structural Genomics

Scott A. Lesley, Ian A. Wilson, I. Wilson, 2005, Journal of Structural and Functional Genomics.

Integration of Inositol Phosphate Signaling Pathways via Human ITPK1*

G. Spraggon, S. Lesley, David H. Jones, 2007, Journal of Biological Chemistry.

The structure of Haemophilus influenzae prephenate dehydrogenase suggests unique features of bifunctional TyrA enzymes

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Estimation of Hydrogen-Exchange Protection Factors from MD Simulation Based on Amide Hydrogen Bonding Analysis

Scott A. Lesley, Glen Spraggon, In-Hee Park, 2015, J. Chem. Inf. Model..

Structure and Function of a Novel ld-Carboxypeptidase A Involved in Peptidoglycan Recycling

Mitchell D. Miller, A. Godzik, A. Deacon, 2013, Journal of bacteriology.

Computational analysis of crystallization trials.

Glen Spraggon, Scott A Lesley, Andreas Kreusch, 2002, Acta crystallographica. Section D, Biological crystallography.

Identification of selective, nonpeptidic nitrile inhibitors of cathepsin s using the substrate activity screening method.

Scott Lesley, Glen Spraggon, G. Spraggon, 2006, Journal of medicinal chemistry.

Structure of Bacteroides thetaiotaomicron BT2081 at 2.05 Å resolution: the first structural representative of a new protein family that may play a role in carbohydrate metabolism

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Functional analysis of substrate and cofactor complex structures of a thymidylate synthase-complementing protein.

Ashley M Deacon, Peter Kuhn, Scott A Lesley, 2003, Structure.

Structural Insights into the Recognition of Phosphopeptide by the FHA Domain of Kanadaptin

A. Deacon, I. Wilson, S. Lesley, 2014, PloS one.

The structure of the first representative of Pfam family PF09836 reveals a two-domain organization and suggests involvement in transcriptional regulation

Slawomir K. Grzechnik, Mitchell D. Miller, N. Grishin, 2009, Acta crystallographica. Section F, Structural biology and crystallization communications.

Crystal structure of the human TRPV2 channel ankyrin repeat domain

Andreas Kreusch, A. Patapoutian, Eric Koesema, 2006, Protein science : a publication of the Protein Society.

Crystal structures of human HSP90alpha-complexed with dihydroxyphenylpyrazoles.

Scott A Lesley, Andreas Kreusch, Yun He, 2005, Bioorganic & medicinal chemistry letters.

Structure of a putative NTP pyrophosphohydrolase: YP_001813558.1 from Exiguobacterium sibiricum 255-15

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Structure of a membrane-attack complex/perforin (MACPF) family protein from the human gut symbiont Bacteroides thetaiotaomicron

Herbert L. Axelrod, Constantina Bakolitsa, Adam Godzik, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Structure and Function of the First Full-Length Murein Peptide Ligase (Mpl) Cell Wall Recycling Protein

Adam Godzik, Hsiu-Ju Chiu, Debanu Das, 2011, PloS one.

Homogeneous High-Throughput Screening Assays for HIV-1 Integrase 3β-Processing and Strand Transfer Activities

J. Caldwell, K. Kuhen, S. Lesley, 2005, Journal of biomolecular screening.

A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells.

Paul W Anderson, S. Lesley, M. Gaylord, 2019, Methods in molecular biology.

Mammalian cell culture density determination using a laser through-beam sensor.

Scott A Lesley, Sarah M Rue, Paul W Anderson, 2018, BioTechniques.

Crystal structure of NMA1982 from Neisseria meningitidis at 1.5 Å resolution provides a structural scaffold for nonclassical, eukaryotic‐like phosphatases

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2007, Proteins.

Structure analysis of peptide deformylases from Streptococcus pneumoniae, Staphylococcus aureus, Thermotoga maritima and Pseudomonas aeruginosa: snapshots of the oxygen sensitivity of peptide deformylase.

G. Spraggon, S. Lesley, A. Kreusch, 2003, Journal of molecular biology.

Correction: Structure of an MmyB-Like Regulator from C. aurantiacus, Member of a New Transcription Factor Family Linked to Antibiotic Metabolism in Actinomycetes

Mitchell D. Miller, A. Godzik, A. Deacon, 2012, PLoS ONE.

Structural insights into enzyme regulation for inositol 1,4,5-trisphosphate 3-kinase B.

Glen Spraggon, Scott A Lesley, M. Cooke, 2005, Biochemistry.

Studies of the role of the Escherichia coli heat shock regulatory protein sigma 32 by the use of monoclonal antibodies.

N. Thompson, R. Burgess, S. Lesley, 1987, The Journal of biological chemistry.

Crystal structure of an Udp‐n‐acetylmuramate‐alanine ligase MurC (TM0231) from Thermotoga maritima at 2.3 Å resolution

Mitchell D. Miller, A. Godzik, A. Deacon, 2004, Proteins.

from in the Gut

A. Godzik, A. Deacon, I. Wilson, 2016 .

Thermotoga maritima of Three-Dimensional Structural View of the Central Metabolic Network

Ines Thiele, Ying Zhang, Adam Godzik, 2011 .

A chromogenic assay for screening large antibody libraries

P. Schultz, B. Gong, S. Lesley, 1992 .

Reactivation of methionine synthase from Thermotoga maritima (TM0268) requires the downstream gene product TM0269

I. Wilson, R. Matthews, S. Lesley, 2007, Protein science : a publication of the Protein Society.

Crystal structure of homoserine O‐succinyltransferase from Bacillus cereus at 2.4 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2007, Proteins.

Crystal Structure and Activity Studies of the C11 Cysteine Peptidase from Parabacteroides merdae in the Human Gut Microbiome

A. Godzik, A. Deacon, I. Wilson, 2016, The Journal of Biological Chemistry.

Crystal structure of the Fic (Filamentation induced by cAMP) family protein SO4266 (gi|24375750) from Shewanella oneidensis MR‐1 at 1.6 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2009, Proteins.

Crystal structure of a glycerate kinase (TM1585) from Thermotoga maritima at 2.70 Å resolution reveals a new fold

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2006, Proteins.

Crystal structure of a type II quinolic acid phosphoribosyltransferase (TM1645) from Thermotoga maritima at 2.50 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins.

Crystal structure of 2‐keto‐3‐deoxygluconate kinase (TM0067) from Thermotoga maritima at 2.05 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2008, Proteins: Structure, Function, and Bioinformatics.

High‐Throughput Cloning, Expression and Purification Technologies

Scott A. Lesley, Andreas Kreusch, S. Lesley, 2005 .

Crystal structure of the ALK (anaplastic lymphoma kinase) catalytic domain.

Sungjoon Kim, Nanxin Li, Jennifer L. Harris, 2010, The Biochemical journal.

Crystal structure of a putative NADPH‐dependent oxidoreductase (GI: 18204011) from mouse at 2.10 Å resolution

Adam Godzik, Henry van den Bedem, Slawomir K. Grzechnik, 2004, Proteins.

A conserved fold for fimbrial components revealed by the crystal structure of a putative fimbrial assembly protein (BT1062) from Bacteroides thetaiotaomicron at 2.2 Å resolution

Mitchell D. Miller, A. Godzik, A. Deacon, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Crystal structure of O‐acetylserine sulfhydrylase (TM0665) from Thermotoga maritima at 1.8 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins: Structure, Function, and Bioinformatics.

Cofactor‐induced reversible folding of Flavodoxin‐4 from Lactobacillus acidophilus

A. Godzik, A. Deacon, K. Wüthrich, 2015, Protein science : a publication of the Protein Society.

Crystal structure of 2‐phosphosulfolactate phosphatase (ComB) from Clostridium acetobutylicum at 2.6 Å resolution reveals a new fold with a novel active site

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2006, Proteins.

Crystal structure of acireductone dioxygenase (ARD) from Mus musculus at 2.06 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2006 .

Crystal structure of virulence factor CJ0248 from Campylobacter jejuni at 2.25 Å resolution reveals a new fold

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2005, Proteins.

Crystal structure of the global regulatory protein CsrA from Pseudomonas putida at 2.05 Å resolution reveals a new fold

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2005, Proteins.

Structural Biology and Crystallization Communications the Structure of Haemophilus Influenzae Prephenate Dehydrogenase Suggests Unique Features of Bifunctional Tyra Enzymes

Mitchell D. Miller, A. Godzik, A. Deacon, 2022 .

Crystal structure of a novel Sm‐like protein of putative cyanophage origin at 2.60 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, K. D. Murphy, 2009, Proteins.

Crystal structure of a formiminotetrahydrofolate cyclodeaminase (TM1560) from Thermotoga maritima at 2.80 Å resolution reveals a new fold

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2005, Proteins: Structure, Function, and Bioinformatics.

Oxidase activity of a flavin‐dependent thymidylate synthase

Zhen Wang, S. Lesley, A. Kohen, 2009, The FEBS journal.

The Structure of Mlc Titration Factor A (MtfA/YeeI) Reveals a Prototypical Zinc Metallopeptidase Related to Anthrax Lethal Factor

Adam Godzik, Hsiu-Ju Chiu, Qingping Xu, 2012, Journal of bacteriology.

Crystal structure of a putative glutamine amido transferase (TM1158) from Thermotoga maritima at 1.7 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins.

Crystal structure of a glycerophosphodiester phosphodiesterase (GDPD) from Thermotoga maritima (TM1621) at 1.60 Å resolution

Slawomir K. Grzechnik, Mitchell D. Miller, A. Godzik, 2004, Proteins.

Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells

Mitchell D. Miller, A. Godzik, A. Deacon, 2015, Proceedings of the National Academy of Sciences.