H. Kuyama

发表

A simple device using magnetic transportation for droplet-based PCR

Tetsuo Ohashi, H. Kuyama, Nobuhiro Hanafusa, 2007, Biomedical microdevices.

Selective isolation of N-terminal peptides from proteins and their de novo sequencing by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry without regard to unblocking or blocking of N-terminal amino acids.

T. Nakazawa, O. Nishimura, H. Kuyama, 2008, Rapid communications in mass spectrometry : RCM.

Computational survey of sequence specificity for protein terminal tags covering nine organisms and its application to protein identification.

Akiyasu C. Yoshizawa, Shigeki Kajihara, Koichi Tanaka, 2015, Journal of proteome research.

Identification of human basic fetoprotein as glucose-6-phosphate isomerase by using N- and C-terminal sequence tags and terminal tag database.

Akiyasu C. Yoshizawa, Koichi Tanaka, H. Kuyama, 2015, Journal of Pharmaceutical and Biomedical Analysis.

C-Terminal sequencing of protein by MALDI mass spectrometry through the specific derivatization of the α-carboxyl group with 3-aminopropyltris-(2,4,6-trimethoxyphenyl)phosphonium bromide

T. Nakazawa, O. Nishimura, H. Kuyama, 2012, Analytical and Bioanalytical Chemistry.

The specific isolation of C-terminal peptides of proteins through a transamination reaction and its advantage for introducing functional groups into the peptide.

Ei-ichi Matsuo, O. Nishimura, H. Kuyama, 2009, Rapid communications in mass spectrometry : RCM.

An improved sample preparation method for the sensitive detection of peptides by MALDI-MS.

Koichi Tanaka, H. Kuyama, Y. Hioki, 2013, Journal of mass spectrometry : JMS.

A method for terminus proteomics: selective isolation and labeling of N-terminal peptide from protein through transamination reaction.

Ei-ichi Matsuo, O. Nishimura, H. Kuyama, 2009, Bioorganic & medicinal chemistry letters.

Enriching C-terminal peptide from endopeptidase ArgC digest for protein C-terminal analysis.

Koichi Tanaka, H. Kuyama, C. Nakajima, 2012, Bioorganic & medicinal chemistry letters.

Mass spectrometry-based sequencing of protein C-terminal peptide using α-carboxyl group-specific derivatization and COOH capturing.

Koichi Tanaka, H. Kuyama, C. Nakajima, 2012, Analytical biochemistry.

Sensitive detection of phosphopeptides by matrix-assisted laser desorption/ionization mass spectrometry: use of alkylphosphonic acids as matrix additives.

O. Nishimura, H. Kuyama, K. Sonomura, 2008, Rapid communications in mass spectrometry : RCM.

Imidazole C-2 Hydrogen/Deuterium Exchange Reaction at Histidine for Probing Protein Structure and Function with MALDI Mass Spectrometry

M. Miyagi, T. Nakazawa, O. Nishimura, 2015 .

Characterization of N- and C-termini of HER2 protein by mass spectrometry-based sequencing: Observation of two types of N-terminal sequence

Koichi Tanaka, H. Kuyama, C. Nakajima, 2014 .

Mass spectrometry based N- and C-terminal sequence determination of a hepatopancreas-type prophenoloxidase from the kuruma prawn, Marsupenaeus japonicus

O. Nishimura, H. Kuyama, T. Hirata, 2013, Analytical and Bioanalytical Chemistry.

A novel type of prophenoloxidase from the kuruma prawn Marsupenaeus japonicus contributes to the melanization of plasma in crustaceans.

O. Nishimura, H. Kuyama, T. Hirata, 2012, Fish & shellfish immunology.

A method for N-terminal de novo sequencing of Nα-blocked proteins by mass spectrometry.

T. Nakazawa, O. Nishimura, H. Kuyama, 2011, The Analyst.

Magnetic particle transport through organogel- an application to DNA extraction.

Tetsuo Ohashi, H. Kuyama, 2020, Analytical biochemistry.

Quantitative profiling of drug-associated proteomic alterations by combined 2-nitrobenzenesulfenyl chloride (NBS) isotope labeling and 2DE/MS identification.

Kun Yu, P. Tan, Ei-ichi Matsuo, 2006, Journal of proteome research.

Fast Separation of Oligonucleotide and Triplet Repeat DNA on a Microfabricated Capillary Electrophoresis Device

Y. Baba, H. Kuyama, H. Nakanishi, 2000 .

Studies on Pteridium aquilinum var. latiusculum, IV. Isolation of three p-hydroxystyrene glycosides and an efficient method for the isolation of ptaquiloside, an unstable bracken carcinogen.

M. Ojika, H. Kuyama, H. Kigoshi, 1985, Journal of natural products.

Cyclo-glycosylation of (1→4)-linked glycohexaoses: Synthesis of cyclo-lactohexaose

H. Kuyama, T. Nukada, T. Ogawa, 1993 .

Cyclo-glycosylation of a (1-->4)-linked glycooctaose and glycodecaose: synthesis of cyclo-lactooctaose and cyclo-lactodecaose.

H. Kuyama, Y. Ito, T. Nukada, 1995, Carbohydrate research.

Stereocontrolled synthesis of chitosan dodecamer.

H. Kuyama, Y. Ito, T. Nukada, 1993, Carbohydrate research.

Development of iodoacetic acid-based cysteine mass tags: detection enhancement for cysteine-containing peptide by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Koichi Tanaka, H. Kuyama, Taka-Aki Sato, 2012, Analytical biochemistry.

A method for N-terminal de novo sequence analysis of proteins by matrix-assisted laser desorption/ionization mass spectrometry.

O. Nishimura, H. Kuyama, K. Sonomura, 2008, Analytical biochemistry.

Selective isolation of N-blocked peptide by combining AspN digestion, transamination, and tosylhydrazine glass treatment.

S. Futaki, Ei-ichi Matsuo, O. Nishimura, 2011, Analytical biochemistry.

A new strategy for protein biomarker discovery utilizing 2-nitrobenzenesulfenyl (NBS) reagent and its applications to clinical samples.

Ei-ichi Matsuo, O. Nishimura, H. Kuyama, 2009, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

High-throughput method for N-terminal sequencing of proteins by MALDI mass spectrometry.

T. Nakazawa, H. Kuyama, T. Okamura, 2005, Analytical chemistry.

Imidazole C-2 hydrogen/deuterium exchange reaction at histidine for probing protein structure and function with matrix-assisted laser desorption ionization mass spectrometry.

M. Miyagi, T. Nakazawa, O. Nishimura, 2014, Biochemistry.

An efficient chemical method for dephosphorylation of phosphopeptides.

O. Nishimura, Koichi Tanaka, H. Kuyama, 2003, Rapid communications in mass spectrometry : RCM.

MALDI mass spectrometry-based sequence analysis of arginine-containing glycopeptides: improved fragmentation of glycan and peptide chains by modifying arginine residue.

Shigeki Kajihara, Koichi Tanaka, H. Kuyama, 2013, Journal of mass spectrometry : JMS.

An improved method for de novo sequencing of arginine-containing, Nalpha-tris(2,4,6-trimethoxyphenyl)phosphonium-acetylated peptides.

O. Nishimura, H. Kuyama, K. Sonomura, 2008, Rapid communications in mass spectrometry : RCM.

A simple and highly successful C‐terminal sequence analysis of proteins by mass spectrometry

O. Nishimura, H. Kuyama, K. Sonomura, 2008, Proteomics.

Enzymatic conversion of arginine to citrulline for improving fragmentation of Nα-tris(2,4,6-trimethoxyphenyl)phosphonium-acetylated peptides by tandem mass spectrometry

T. Nakazawa, O. Nishimura, H. Kuyama, 2011 .

Conversion of arginine to ornithine for improving the fragmentation pattern of peptides labeled with the N-terminal tris(2,4,6-trimethoxyphenyl)phosphonium group in tandem mass spectrometry

T. Nakazawa, O. Nishimura, H. Kuyama, 2010 .

Specific isolation of N-terminal fragments from proteins and their high-fidelity de novo sequencing.

T. Nakazawa, O. Nishimura, H. Kuyama, 2007, Rapid communications in mass spectrometry : RCM.

High sequence-coverage detection of proteolytic peptides using a bis(terpyridine)ruthenium(II) complex.

T. Nakazawa, Hitoshi Yamamoto, R. Masui, 2007, The Analyst.

Application of Bis(terpyridine)ruthenium(II) to N-Terminal Amino Acid Sequencing

T. Nakazawa, Hitoshi Yamamoto, R. Masui, 2005 .

Simultaneous detection of N-terminal fragment ions in a protein mixture using a ruthenium(II) complex.

T. Nakazawa, Hitoshi Yamamoto, R. Masui, 2007, Rapid Communications in Mass Spectrometry.

Control of aqueous droplets using magnetic and electrostatic forces.

Tetsuo Ohashi, H. Kuyama, Koichi Suzuki, 2008, Analytica chimica acta.

Laser-induced Propagation and Destruction of Amyloid β Fibrils*

T. Kawakami, O. Nishimura, H. Kuyama, 2010, The Journal of Biological Chemistry.

Rapid and sensitive amino-acid sequencing of cloning Thermus thermophilus HB8 ferredoxin by proteomics.

T. Nakazawa, Hitoshi Yamamoto, R. Masui, 2004, Journal of proteome research.

An approach to quantitative proteome analysis by labeling tryptophan residues.

O. Nishimura, H. Kuyama, E. Ando, 2003, Rapid communications in mass spectrometry : RCM.

Selective isolation of N-blocked peptide by combining AspN digestion, transamination, and tosylhydrazine glass treatment.

S. Futaki, Ei-ichi Matsuo, O. Nishimura, 2011, Analytical biochemistry.