J. Broderick

发表

Evidence for retention of biological activity of a non-heme iron enzyme adsorbed on a silver colloid: a surface-enhanced resonance Raman scattering study.

M. Natan, R. V. Van Duyne, T. O’Halloran, 1993, Biochemistry.

Insights into [FeFe]-hydrogenase structure, mechanism, and maturation.

J. W. Peters, M. Posewitz, P. King, 2011, Structure.

[FeFe]- and [NiFe]-hydrogenase diversity, mechanism, and maturation.

J. W. Peters, P. King, E. Boyd, 2015, Biochimica et biophysica acta.

Biosynthesis of complex iron-sulfur enzymes.

J. W. Peters, E. Boyd, J. Broderick, 2011, Current opinion in chemical biology.

Radical SAM Enzymes and Their Roles in Complex Cluster Assembly

J. Broderick, E. Shepard, J. Betz, 2015 .

Radical S-Adenosyl-l-methionine Chemistry in the Synthesis of Hydrogenase and Nitrogenase Metal Cofactors*

J. W. Peters, A. Byer, J. Broderick, 2014, The Journal of Biological Chemistry.

Radical SAM enzymes in methylation and methylthiolation.

J. Broderick, Rachel U. Hutcheson, 2012, Metallomics : integrated biometal science.

11 Catechol dioxygenases

J. Broderick, 2003 .

Overproduction, purification, and characterization of chlorocatechol dioxygenase, a non-heme iron dioxygenase with broad substrate tolerance.

T. O’Halloran, J. Broderick, Joan B. Broderick, 1991, Biochemistry.

Mechanism of Radical S-Adenosyl-l-methionine Adenosylation: Radical Intermediates and the Catalytic Competence of the 5'-Deoxyadenosyl Radical.

B. Hoffman, J. Broderick, W. A. van der Donk, 2022, Journal of the American Chemical Society.

The Radical SAM enzyme Spore Photoproduct Lyase: Properties of the Ω Organometallic Intermediate and Identification of Stable Protein Radicals Formed During Substrate-Free Turnover.

B. Hoffman, J. Broderick, E. Shepard, 2020, Journal of the American Chemical Society.

The Elusive 5'-Deoxyadenosyl Radical: Captured and Characterized by EPR and ENDOR Spectroscopies.

A. Byer, K. Yokoyama, B. Hoffman, 2019, Journal of the American Chemical Society.

EPR and FTIR analysis of the mechanism of H2 activation by [FeFe]-hydrogenase HydA1 from Chlamydomonas reinhardtii.

J. W. Peters, A. Byer, P. King, 2013, Journal of the American Chemical Society.

Hydrogenase cluster biosynthesis: organometallic chemistry nature's way.

J. W. Peters, David W. Mulder, J. Broderick, 2009, Dalton transactions.

S K-edge XAS and DFT calculations on SAM dependent pyruvate formate-lyase activating enzyme: nature of interaction between the Fe4S4 cluster and SAM and its role in reactivity.

K. Hodgson, J. Broderick, A. Dey, 2011, Journal of the American Chemical Society.

Frontiers in enzymatic C-H-bond activation.

J. Bollinger, J. Broderick, 2009, Current opinion in chemical biology.

Spectroscopic Investigation of Pyruvate Formate Lyase-activating Enzyme: A Look into EPR, ENDOR and Mossabuer Spectroscopy

J. Broderick, Danilo O. Ortillo, 2014 .

Radical S-Adenosyl-l-Methionine Enzymes

J. Broderick, Maike N Lundahl, W. E. Broderick, 2021 .

Radical SAM enzymes: surprises along the path to understanding mechanism

J. Broderick, W. E. Broderick, W. Broderick, 2019, JBIC Journal of Biological Inorganic Chemistry.

Radical SAM catalysis via an organometallic intermediate with an Fe–[5′-C]-deoxyadenosyl bond

B. Hoffman, J. Broderick, Amy R. Marts, 2016, Science.

Biochemistry: A radically different enzyme

J. Broderick, 2010, Nature.

Complete stereospecific repair of a synthetic dinucleotide spore photoproduct by spore photoproduct lyase

J. Broderick, E. Zilinskas, W. E. Broderick, 2010, JBIC Journal of Biological Inorganic Chemistry.

Control of radical chemistry in the AdoMet radical enzymes.

J. Broderick, Kaitlin S. Duschene, Sunshine C. Silver, 2009, Current opinion in chemical biology.

Electron-nuclear double resonance spectroscopic evidence that S-adenosylmethionine binds in contact with the catalytically active [4Fe-4S](+) cluster of pyruvate formate-lyase activating enzyme.

B. Hoffman, J. Broderick, C. Walsby, 2002, Journal of the American Chemical Society.

Escherichia coli LipA is a lipoyl synthase: in vitro biosynthesis of lipoylated pyruvate dehydrogenase complex from octanoyl-acyl carrier protein.

J. Cronan, M. Marletta, J. Broderick, 2000, Biochemistry.

HydG, the "dangler" iron, and catalytic production of free CO and CN-: implications for [FeFe]-hydrogenase maturation.

J. W. Peters, A. Byer, J. Broderick, 2021, Dalton transactions.

[FeFe]-hydrogenase maturation: H-cluster assembly intermediates tracked by electron paramagnetic resonance, infrared, and X-ray absorption spectroscopy

J. Broderick, M. Haumann, A. Magnuson, 2020, JBIC Journal of Biological Inorganic Chemistry.

Electron Spin Relaxation and Biochemical Characterization of the Hydrogenase Maturase HydF: Insights into [2Fe-2S] and [4Fe-4S] Cluster Communication and Hydrogenase Activation

A. Byer, J. Broderick, S. Eaton, 2017, Biochemistry.

A Redox Active [2Fe-2S] Cluster on the Hydrogenase Maturase HydF.

J. W. Peters, A. Byer, J. Broderick, 2016, Biochemistry.

[FeFe]-hydrogenase maturation.

J. W. Peters, A. Byer, J. Broderick, 2014, Biochemistry.

[FeFe]‐Hydrogenase Cofactor Assembly

J. W. Peters, A. Byer, E. Boyd, 2013 .

Iron–sulfur cluster coordination in the [FeFe]‐hydrogenase H cluster biosynthetic factor HydF

J. W. Peters, A. Byer, J. Broderick, 2012, FEBS letters.

Radical AdoMet enzymes in complex metal cluster biosynthesis.

J. W. Peters, E. Boyd, J. Broderick, 2012, Biochimica et biophysica acta.

Identification and Characterization of a Novel Member of the Radical AdoMet Enzyme Superfamily and Implications for the Biosynthesis of the Hmd Hydrogenase Active Site Cofactor

J. W. Peters, E. Boyd, J. Broderick, 2009, Journal of bacteriology.

Activation of HydA Δ EFG Requires a Preformed [ 4 Fe-4 S ] Cluster †

J. W. Peters, David W. Mulder, J. Broderick, 2009 .

Photoinduced Electron Transfer in a Radical SAM Enzyme Generates an S-Adenosylmethionine Derived Methyl Radical.

B. Hoffman, J. Broderick, E. Shepard, 2019, Journal of the American Chemical Society.

Proteomic Analysis of Methanococcus voltae Grown in the Presence of Mineral and Nonmineral Sources of Iron and Sulfur

J. Broderick, B. Bothner, E. Shepard, 2022, Microbiology spectrum.

[FeFe]-hydrogenase cyanide ligands derived from S-adenosylmethionine-dependent cleavage of tyrosine.

J. W. Peters, E. Boyd, P. Roach, 2010, Angewandte Chemie.

Radical S-adenosylmethionine maquette chemistry: Cx3Cx2C peptide coordinated redox active [4Fe–4S] clusters

V. Copié, J. Broderick, Jacquelyn Miller, 2019, JBIC Journal of Biological Inorganic Chemistry.

Active-Site Controlled, Jahn-Teller Enabled Regioselectivity in Reductive S-C Bond Cleavage of S-Adenosylmethionine in Radical SAM Enzymes.

B. Hoffman, J. Broderick, E. Shepard, 2020, Journal of the American Chemical Society.

Monovalent Cation Activation of the Radical SAM Enzyme Pyruvate Formate-Lyase Activating Enzyme

A. Byer, B. Hoffman, J. Broderick, 2017, Journal of the American Chemical Society.

An anchoring role for FeS clusters: chelation of the amino acid moiety of S-adenosylmethionine to the unique iron site of the [4Fe-4S] cluster of pyruvate formate-lyase activating enzyme.

B. Hoffman, J. Broderick, C. Walsby, 2002, Journal of the American Chemical Society.

Conversion of 3Fe-4S to 4Fe-4S Clusters in Native Pyruvate Formate-Lyase Activating Enzyme: Mössbauer Characterization and Implications for Mechanism

C. Krebs, J. Broderick, B. Huynh, 2000 .

H-cluster assembly intermediates built on HydF by the radical SAM enzymes HydE and HydG

A. Byer, P. King, J. Broderick, 2019, JBIC Journal of Biological Inorganic Chemistry.

Characterization of the Preprocessed Copper Site Equilibrium in Amine Oxidase and Assignment of the Reactive Copper Site in Topaquinone Biogenesis.

Doreen E. Brown, D. Dooley, J. Broderick, 2019, Journal of the American Chemical Society.

Coordination of adenosylmethionine to a unique iron site of the [4Fe-4S] of pyruvate formate-lyase activating enzyme: a Mössbauer spectroscopic study.

C. Krebs, J. Broderick, B. Huynh, 2002, Journal of the American Chemical Society.

The iron-sulfur cluster of pyruvate formate-lyase activating enzyme in whole cells: cluster interconversion and a valence-localized [4Fe-4S]2+ state.

J. Broderick, M. Li, S. Naik, 2009, Biochemistry.

HydF as a scaffold protein in [FeFe] hydrogenase H‐cluster biosynthesis

Mark A. Winslow, J. W. Peters, M. Posewitz, 2008, FEBS letters.

[FeFe]-hydrogenase maturation: HydG-catalyzed synthesis of carbon monoxide.

J. W. Peters, P. Roach, J. Broderick, 2010, Journal of the American Chemical Society.

Chemoselective Deprotection of Triethylsilyl Ethers

J. Broderick, W. E. Broderick, T. Chandra, 2009, Nucleosides, nucleotides & nucleic acids.

Paradigm Shift for Radical S-Adenosyl-l-methionine Reactions: The Organometallic Intermediate Ω Is Central to Catalysis

L. Scott, A. Byer, K. Yokoyama, 2018, Journal of the American Chemical Society.

S-Adenosyl-l-ethionine is a Catalytically Competent Analog of S-Adenosyl-l-methione (SAM) in the Radical SAM Enzyme HydG.

B. Hoffman, J. Broderick, E. Shepard, 2021, Angewandte Chemie.

Spore photoproduct lyase catalyzes specific repair of the 5R but not the 5S spore photoproduct.

J. Broderick, E. Shepard, E. Zilinskas, 2009, Journal of the American Chemical Society.

In vitro activation of [FeFe] hydrogenase: new insights into hydrogenase maturation

J. W. Peters, M. Posewitz, P. King, 2007, JBIC Journal of Biological Inorganic Chemistry.

Combined Mössbauer spectroscopic, multi-edge X-ray absorption spectroscopic, and density functional theoretical study of the radical SAM enzyme spore photoproduct lyase

J. Broderick, D. Gardenghi, S. Naik, 2014, JBIC Journal of Biological Inorganic Chemistry.

Pyruvate formate-lyase activating enzyme: elucidation of a novel mechanism for glycyl radical formation.

J. Broderick, Jeffrey M Buis, 2005, Archives of biochemistry and biophysics.

An Efficient Deprotection of N-Trimethylsilylethoxymethyl (SEM) Groups From Dinucleosides and Dinucleotides

J. Broderick, W. E. Broderick, T. Chandra, 2010, Nucleosides, nucleotides & nucleic acids.

The Elusive 5'-Deoxyadenosyl Radical: Captured and Characterized by EPR and ENDOR Spectroscopies.

A. Byer, K. Yokoyama, B. Hoffman, 2019, Journal of the American Chemical Society.

The [4Fe-4S]1+ Cluster of Pyruvate Formate-Lyase Activating Enzyme Generates the Glycyl Radical on Pyruvate Formate-Lyase: EPR-Detected Single Turnover

J. Broderick, T. Henshaw, A. Cheek, 2000 .

Pyruvate Formate-Lyase Activating Enzyme Is an Iron-Sulfur Protein

J. Broderick, Michael K. Johnson, T. Henshaw, 1997 .

Radical S-Adenosylmethionine Enzymes

J. Broderick, E. Shepard, Benjamin R. Duffus, 2014, Chemical reviews.

Emerging paradigms for complex iron-sulfur cofactor assembly and insertion.

J. W. Peters, J. Broderick, 2012, Annual review of biochemistry.

Synthesis of the 2Fe subcluster of the [FeFe]-hydrogenase H cluster on the HydF scaffold

Mark A. Winslow, J. W. Peters, J. Broderick, 2010, Proceedings of the National Academy of Sciences.

Catechol dioxygenases.

J. Broderick, 1999, Essays in biochemistry.

[FeFe]-hydrogenase maturation: insights into the role HydE plays in dithiomethylamine biosynthesis.

Eyal Akiva, Gemma L. Holliday, Patricia C Babbitt, 2015, Biochemistry.

Examining Pathways of Iron and Sulfur Acquisition, Trafficking, Deployment, and Storage in Mineral-Grown Methanogen Cells

E. Boyd, M. Young, J. Broderick, 2021, Journal of bacteriology.

Reversible H Atom Abstraction Catalyzed by the Radical S-Adenosylmethionine Enzyme HydG

J. W. Peters, J. Broderick, Benjamin R. Duffus, 2014, Journal of the American Chemical Society.

Characterization of an Active Spore Photoproduct Lyase, a DNA Repair Enzyme in the Radical S-Adenosylmethionine Superfamily*

J. Broderick, Efthalia Kalliri, J. Cheek, 2006, Journal of Biological Chemistry.

Direct H atom abstraction from spore photoproduct C-6 initiates DNA repair in the reaction catalyzed by spore photoproduct lyase: evidence for a reversibly generated adenosyl radical intermediate.

J. Broderick, J. Cheek, 2002, Journal of the American Chemical Society.

S -Adenosylmethionine and Iron–Sulfur Clusters in Biological Radical Reactions: The Radical SAM Superfamily

J. Broderick, E. Shepard, 2010 .

Biochemical and kinetic characterization of radical S-adenosyl-L-methionine enzyme HydG.

J. W. Peters, P. Roach, J. Broderick, 2013, Biochemistry.

Biochemical and kinetic characterization of radical AdoMet enzyme HydG

J. W. Peters, P. Roach, J. Broderick, 2022 .

17 Origin and evolution of Fe-S proteins and enzymes

E. Boyd, M. Adams, J. Broderick, 2017 .

[FeFe]-Hydrogenase: Defined Lysate-Free Maturation Reveals a Key Role for Lipoyl-H-Protein in DTMA Ligand Biosynthesis.

B. Hoffman, J. Broderick, E. Shepard, 2022, Angewandte Chemie.

The antiviral protein viperin is a radical SAM enzyme

J. Broderick, Kaitlin S. Duschene, 2010, FEBS letters.

Pyruvate Formate-lyase and Its Activation by Pyruvate Formate-lyase Activating Enzyme*

J. Broderick, Adam V Crain, 2013, The Journal of Biological Chemistry.

Mechanistic Studies of Radical SAM Enzymes: Pyruvate Formate-Lyase Activating Enzyme and Lysine 2,3-Aminomutase Case Studies.

A. Byer, J. Broderick, W. E. Broderick, 2018, Methods in enzymology.

Stepwise [FeFe]-hydrogenase H-cluster assembly revealed in the structure of HydAΔEFG

J. W. Peters, E. Boyd, David W. Mulder, 2010, Nature.

[FeFe]-Hydrogenase In Vitro Maturation.

J. Broderick, E. Shepard, Adrien Pagnier, 2022, Angewandte Chemie.

[FeFe]-hydrogenase oxygen inactivation is initiated at the H cluster 2Fe subcluster.

J. W. Peters, P. King, J. Artz, 2015, Journal of the American Chemical Society.

Why Nature Uses Radical SAM Enzymes so Widely: Electron Nuclear Double Resonance Studies of Lysine 2,3-Aminomutase Show the 5'-dAdo• "Free Radical" Is Never Free.

A. Byer, B. Hoffman, J. Broderick, 2015, Journal of the American Chemical Society.

Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction

J. Tiedje, T. Marsh, J. Broderick, 2012, BMC Microbiology.

Adenosylmethionine-dependent iron-sulfur enzymes: versatile clusters in a radical new role

J. Broderick, J. Cheek, 2001, JBIC Journal of Biological Inorganic Chemistry.

Secondary structure analysis of peptides with relevance to iron–sulfur cluster nesting

Rebecca Hanscam, Eric M. Shepard, Joan B. Broderick, 2018, J. Comput. Chem..

Structural basis for glycyl radical formation by pyruvate formate-lyase activating enzyme

J. Broderick, M. Li, C. Drennan, 2008, Proceedings of the National Academy of Sciences.

Glycyl radical activating enzymes: structure, mechanism, and substrate interactions.

J. Broderick, Krista A Shisler, Joan B Broderick, 2014, Archives of biochemistry and biophysics.

Inactivation of E. coli pyruvate formate-lyase: role of AdhE and small molecules.

J. Broderick, Yi Peng, Mbako R Nnyepi, 2007, Archives of biochemistry and biophysics.

8.27 – Iron–Sulfur Clusters in Enzyme Catalysis

J. Broderick, 2003 .

Pyruvate Formate-lyase, Evidence for an Open Conformation Favored in the Presence of Its Activating Enzyme*

J. Broderick, G. Gillispie, Yi Peng, 2010, The Journal of Biological Chemistry.

Pyruvate formate-lyase-activating enzyme: strictly anaerobic isolation yields active enzyme containing a [3Fe-4S](+) cluster.

A. Kopf, J. Broderick, T. Henshaw, 2000, Biochemical and biophysical research communications.

Flavodoxin cofactor binding induces structural changes that are required for protein-protein interactions with NADP(+) oxidoreductase and pyruvate formate-lyase activating enzyme.

J. Broderick, Joan B Broderick, Adam V Crain, 2013, Biochimica et biophysica acta.

Assembling iron-sulfur clusters in the cytosol.

J. Broderick, 2007, Nature chemical biology.

Structural studies of the interaction of S‐adenosylmethionine with the [4Fe‐4S] clusters in biotin synthase and pyruvate formate‐lyase activating enzyme

J. Broderick, W. Hong, N. Cosper, 2003, Protein science : a publication of the Protein Society.

Computational engineering of previously crystallized pyruvate formate-lyase activating enzyme reveals insights into SAM binding and reductive cleavage

J. Broderick, C. M. Lawrence, J. Moody, 2023, The Journal of biological chemistry.

Radical SAM enzymes: Nature's choice for radical reactions

B. Hoffman, J. Broderick, W. Broderick, 2022, FEBS letters.

The B12-independent glycerol dehydratase activating enzyme from Clostridium butyricum cleaves SAM to produce 5'-deoxyadenosine and not 5'-deoxy-5'-(methylthio)adenosine.

J. Broderick, E. Shepard, M. Villanueva, 2021, Journal of inorganic biochemistry.

Mechanism of Radical Initiation in the Radical S-Adenosyl-l-methionine Superfamily.

B. Hoffman, J. Broderick, W. Broderick, 2018, Accounts of chemical research.

Spectroscopic approaches to elucidating novel iron-sulfur chemistry in the "radical-Sam" protein superfamily.

B. Hoffman, J. Broderick, C. Walsby, 2005, Inorganic chemistry.

Solution phase dynamics of the DNA repair enzyme spore photoproduct lyase as probed by H/D exchange

B. Bothner, J. Broderick, Shourjo Ghose, 2014, FEBS letters.

H-Cluster assembly during maturation of the [FeFe]-hydrogenase

J. W. Peters, A. Byer, J. Broderick, 2014, JBIC Journal of Biological Inorganic Chemistry.

Viperin: a radical response to viral infection

J. Broderick, Kaitlin S. Duschene, 2012, Biomolecular concepts.

Activation of HydA(DeltaEFG) requires a preformed [4Fe-4S] cluster.

J. W. Peters, David W. Mulder, J. Broderick, 2009, Biochemistry.

Coenzymes and Cofactors

Joan B. Broderick, J. Broderick, 2001 .

Cyanide and Carbon Monoxide Ligand Formation in Hydrogenase Biosynthesis

J. W. Peters, J. Broderick, K. D. Swanson, 2011 .

Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction

J. Tiedje, T. Marsh, J. Broderick, 2012, BMC Microbiology.

Emerging themes in radical SAM chemistry.

J. Broderick, Krista A Shisler, Joan B Broderick, 2012, Current opinion in structural biology.

Computational Description of Alkylated Iron-Sulfur Organometallic Clusters.

B. Hoffman, J. Broderick, Martín A. Mosquera, 2023, Journal of the American Chemical Society.

Compositional and structural insights into the nature of the H-cluster precursor on HydF.

A. Byer, P. King, David W. Mulder, 2018, Dalton transactions.

Mechanism of Radical Initiation in the Radical SAM Enzyme Superfamily.

B. Hoffman, J. Broderick, W. Broderick, 2023, Annual review of biochemistry.

Faculty Opinions recommendation of Crystallographic and spectroscopic characterization of a nonheme Fe(IV)-O complex.

J. Broderick, 2003 .

Semisynthetic maturation of [FeFe]-hydrogenase using [Fe2(μ-SH)2(CN)2(CO)4]2-: key roles for HydF and GTP.

J. Broderick, Michael T. Mock, E. Shepard, 2023, Chemical communications.

Control of radical chemistry in the AdoMet radical enzymes.

J. Broderick, Kaitlin S. Duschene, Sunshine C. Silver, 2009, Current opinion in chemical biology.

The antiviral protein viperin is a radical SAM enzyme

J. Broderick, Kaitlin S. Duschene, 2010, FEBS letters.

[FeFe]-hydrogenase cyanide ligands derived from S-adenosylmethionine-dependent cleavage of tyrosine.

J. W. Peters, E. Boyd, P. Roach, 2010, Angewandte Chemie.

Pyruvate Formate-lyase, Evidence for an Open Conformation Favored in the Presence of Its Activating Enzyme*

J. Broderick, G. Gillispie, Yi Peng, 2010, The Journal of Biological Chemistry.

ENDOR Spectroscopy Reveals the "Free" 5'-Deoxyadenosyl Radical in a Radical SAM Enzyme Active Site Actually is Chaperoned by Close Interaction with the Methionine-Bound [4Fe-4S]2+ Cluster.

J. Broderick, Martín A. Mosquera, Madeline B Ho, 2024, Journal of the American Chemical Society.

S K-edge XAS and DFT calculations on SAM dependent pyruvate formate-lyase activating enzyme: nature of interaction between the Fe4S4 cluster and SAM and its role in reactivity.

K. Hodgson, J. Broderick, A. Dey, 2011, Journal of the American Chemical Society.

A shift between mineral and nonmineral sources of iron and sulfur causes proteome-wide changes in Methanosarcina barkeri

J. Broderick, Rachel L. Spietz, B. Bothner, 2024, Microbiology spectrum.