D. Umeno

发表

Generating mutant libraries using error-prone PCR.

Patrick C Cirino, P. Cirino, K. Mayer, 2003, Methods in molecular biology.

Directed evolution of squalene synthase for dehydrosqualene biosynthesis

D. Umeno, Kyoichi Saito, Maiko Furubayashi, 2014, FEBS letters.

Production of squalene by squalene synthases and their truncated mutants in Escherichia coli.

D. Umeno, Kyoichi Saito, Maiko Furubayashi, 2015, Journal of bioscience and bioengineering.

A C35 Carotenoid Biosynthetic Pathway

Frances H. Arnold, F. Arnold, D. Umeno, 2003, Applied and Environmental Microbiology.

Diversifying Carotenoid Biosynthetic Pathways by Directed Evolution

Frances H. Arnold, F. Arnold, D. Umeno, 2005, Microbiology and Molecular Biology Reviews.

Molecular breeding of carotenoid biosynthetic pathways

Frances H. Arnold, Claudia Schmidt-Dannert, C. Schmidt-Dannert, 2000, Nature Biotechnology.

Astaxanthin production in a model cyanobacterium Synechocystis sp. PCC 6803.

M. Ikeuchi, S. Takaichi, D. Umeno, 2020, The Journal of general and applied microbiology.

A novel carotenoid biosynthetic route via oxidosqualene.

T. Maoka, D. Umeno, Kyoichi Saito, 2022, Biochemical and biophysical research communications.

Construction of carotenoid biosynthetic pathways using squalene synthase

D. Umeno, Kyoichi Saito, Maiko Furubayashi, 2014, FEBS letters.

Rapid Diversification of BetI-Based Transcriptional Switches for the Control of Biosynthetic Pathways and Genetic Circuits.

D. Umeno, Kyoichi Saito, S. Kawai-Noma, 2016, ACS synthetic biology.

Directed evolution of carotenoid synthases for the production of unnatural carotenoids.

D. Umeno, Maiko Furubayashi, 2012, Methods in molecular biology.

Method to protect a targeted amino acid residue during random mutagenesis.

F. Arnold, K. Hiraga, D. Umeno, 2003, Nucleic acids research.

Improvement of the dP-nucleoside-mediated herpes simplex virus thymidine kinase negative-selection system by manipulating dP metabolism genes.

D. Umeno, Kyoichi Saito, S. Kawai-Noma, 2020, Journal of bioscience and bioengineering.

Dependence of Lanthanide-Ion Binding Performance on HDEHP Concentration in HDEHP Impregnation to Porous Sheet

D. Umeno, Kyoichi Saito, S. Otosaka, 2012 .

Low-threshold amplified UV emission of optically pumped ZnO-polymer nanocomposites

V. C. Agulto, D. Umeno, K. Yamanoi, 2021, Journal of Crystal Growth.

Preparation of Anion-exchange Fibers with Radiation-induced Emulsion Graft Polymerization for Rapid Protein Purification

D. Umeno, Kyoichi Saito, S. Kawai-Noma, 2017 .

Genetically engineered biosynthetic pathways for nonnatural C60 carotenoids using C5-elongases and C50-cyclases in Escherichia coli

T. Maoka, D. Umeno, Kyoichi Saito, 2019, Scientific Reports.

Immobilization of an Esterase Inhibitor on a Porous Hollow-Fiber Membrane by Radiation-Induced Graft Polymerization for Developing a Diagnostic Tool for Feline Kidney Diseases

D. Umeno, T. Yamashita, Kyoichi Saito, 2013, Bioscience, biotechnology, and biochemistry.

Transcription factors as evolvable biosensors.

D. Umeno, S. Kawai-Noma, Yuki Kimura, 2021, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

Directed evolution of transcriptional switches using dual-selector systems.

D. Umeno, Yuki Kimura, 2020, Methods in enzymology.

Affinity adsorption separation of mutagenic molecules by polyacrylamide hydrogels comprising double-stranded DNA

M. Maeda, D. Umeno, Takeshi Kano, 1998 .

Nonnatural biosynthetic pathway for 2-hydroxylated xanthophylls with C50-carotenoid backbone.

N. Misawa, T. Maoka, D. Umeno, 2019, Journal of bioscience and bioengineering.

A methodology for creating thermostabilized mutants of G‐protein coupled receptors by combining statistical thermodynamics and evolutionary molecular engineering

Tomohiko Hayashi, M. Kinoshita, T. Matsuura, 2022, Protein Science.

Effect of Dose on Amount of Protein Adsorbed on Anion-Exchange Fibers Prepared by Radiation-Induced Emulsion Graft Polymerization

D. Umeno, Kyoichi Saito, S. Kawai-Noma, 2019, RADIOISOTOPES.

Adsorption of Catechin in Green-Tea Extracts Using N-Vinylacetamide-Grafted Fiber

D. Umeno, Kyoichi Saito, T. Sugo, 2018, RADIOISOTOPES.

Improvement of protein binding capacity of acrylic-acid-grafted fibers by polymer root-to-brush shift

D. Umeno, Kyoichi Saito, S. Kawai-Noma, 2019, Radiation Physics and Chemistry.

Acrylic Acid-grafted Fibers Enable High-capacity Binding of Lysozyme Dissolved in High-concentration Phosphate Buffer

D. Umeno, Kyoichi Saito, S. Kawai-Noma, 2018, RADIOISOTOPES.

Preparation of Cation-Exchange Fibers with High Protein-Binding Capacities by Pre-Irradiation Induced Emulsion Graft Polymerization

T. Kojima, D. Umeno, Kyoichi Saito, 2017 .

Determination of Mole Percentages of Brush and Root of Polymer Chain Grafted onto Porous Sheet

S. Uchiyama, D. Umeno, Kyoichi Saito, 2013 .

Dependence of protein binding capacity of dimethylamino-γ-butyric-acid (DMGABA)-immobilized porous membrane on composition of solvent used for DMGABA immobilization

D. Umeno, Kyoichi Saito, T. Sugo, 2013 .

Effect of Dose on Mole Percentages of Polymer Brush and Root Grafted onto Porous Polyethylene Sheet by Radiation-Induced Graft Polymerization

S. Uchiyama, D. Umeno, Kyoichi Saito, 2013 .

Erratum: Engineering proteins that bind, move, make and break DNA (Current Opinion in Biotechnology (August 2003) 14 (371-378) pii: S0958166903000910)

F. Arnold, C. Collins, Y. Yokobayashi, 2003 .

Temperature-Induced Precipitation of Specific DNA Fragments Using DNA-Poly(N-isopropylacrylamide) Conjugate

M. Maeda, D. Umeno, 1999 .

Evolution of a Pathway to Novel Long-Chain Carotenoids

F. Arnold, D. Umeno, 2004, Journal of bacteriology.

Effect of Chelating Group Density of Crosslinked Graft Chain on Dynamic Binding Capacity for Metal Ions

D. Umeno, Kyoichi Saito, Ryo Ishihara, 2011 .

Evolutionary Design of Choline-Inducible and -Repressible T7-Based Induction Systems.

Satoshi Koizumi, Kohei Ike, Kyoichi Saito, 2015, ACS synthetic biology.

Crosslinked-Chelating Porous Sheet with High Dynamic Binding Capacity of Metal Ions

D. Umeno, Kyoichi Saito, Ryo Ishihara, 2013 .

Preparation of Extractant-Impregnated Fiber for Recovery of Palladium from Hydrochloric Acid Solution

D. Umeno, Kyoichi Saito, T. Sugo, 2016 .

Electrodialysis of Sulfuric Acid with Cation-Exchange Membranes Prepared by Electron-Beam-Induced Graft Polymerization

D. Umeno, Kyoichi Saito, K. Miyoshi, 2011 .

Pathways by Directed Evolution Diversifying Carotenoid Biosynthetic

F. Arnold, D. Umeno, A. V. Tobias, 2005 .

Simple Method for High-Density Impregnation of Aliquat 336 onto Porous Sheet and Binding Performance of Resulting Sheet for Palladium Ions

D. Umeno, Kyoichi Saito, Ryo Ishihara, 2014 .

High-performance collection of palladium ions in acidic media using nucleic-acid-base-immobilized porous hollow-fiber membranes

D. Umeno, Kyoichi Saito, T. Sugo, 2008 .

High-throughput solid-phase extraction of metal ions using an iminodiacetate chelating porous disk prepared by graft polymerization.

D. Umeno, Kyoichi Saito, T. Sugo, 2007, Journal of chromatography. A.

Protein Binding to Amphoteric Polymer Brushes Grafted onto a Porous Hollow‐Fiber Membrane

D. Umeno, Kyoichi Saito, T. Sugo, 2007, Biotechnology progress (Print).

Evolution of the C30 Carotenoid Synthase CrtM for Function in a C40 Pathway

F. Arnold, D. Umeno, A. V. Tobias, 2002, Journal of bacteriology.

High-resolution separation of neodymium and dysprosium ions utilizing extractant-impregnated graft-type particles.

S. Uchiyama, D. Umeno, Kyoichi Saito, 2018, Journal of chromatography. A.

Nd/Dy Resolution by SPE-Based Elution Chromatography with Bis(2-ethylhexyl) Phosphate (HDEHP)-Impregnated Fiber-Packed Bed

S. Uchiyama, D. Umeno, Kyoichi Saito, 2015 .

Modification of a hydrophobic-ligand-containing porous sheet using tri-n-octylphosphine oxide, and its adsorption/elution of bismuth ions

D. Umeno, Kyoichi Saito, Ryo Ishihara, 2010 .

Protein Resolution in Elution Chromatography Using Novel Cation-Exchange Polymer-Brush-Immobilized Particles

D. Umeno, Kyoichi Saito, N. Shinohara, 2012 .

Removal of Cesium Using Cobalt-Ferrocyanide-Impregnated Polymer-Chain-Grafted Fibers

T. Kojima, S. Uchiyama, D. Umeno, 2011 .

【Original Contribution】 Reduction of Buffer Volume Used in Regeneration ofAnion–Exchange Porous Hollow–Fiber Membrane by Site–ControlledIntroduction of Anion–Exchange Group into Graft Chain

D. Umeno, Kyoichi Saito, S. Kawai-Noma, 2014 .

Removal of Boron Using Nylon-Based Chelating Fibers

D. Umeno, Kyoichi Saito, T. Sugo, 2011 .

Binding of Phosphotyrosine to Gallium-Ion-Immobilized Porous Hollow-Fiber Membrane

D. Umeno, Kyoichi Saito, T. Sugo, 2010 .

Carboxybetaine–Group Immobilized onto Pore Surface Reduced Protein Adsorption to Porous Membrane

D. Umeno, Kyoichi Saito, M. Sakamoto, 2010 .

Purification of His-Tagged Protein Using an Immobilized Nickel Affinity Porous Hollow-Fiber Membrane

D. Umeno, Kyoichi Saito, T. Sugo, 2009 .

Protein Purification Using Immobilized Metal Affinity Porous Sheet

D. Umeno, Kyoichi Saito, T. Sugo, 2008 .

Preparation of Extractant-impregnated Porous Sheets for High-speed Separation of Radionuclides

N. Shinohara, D. Umeno, Kyoichi Saito, 2007 .

Protein Binding Characteristics of Amphoteric Polymer Brushes Grafted onto Porous Hollow-Fiber Membrane

D. Umeno, Kyoichi Saito, T. Sugo, 2007 .

Selection of solvents suitable for immobilization of N-methyglucamine on poly(glycidyl methacrylate) grafted onto nylon fiber

D. Umeno, Kyoichi Saito, T. Sugo, 2011 .

Preparation of Size–Exclusion Polymer Chain Grafted onto the Pore Surface of a Porous Hollow–Fiber Membrane

D. Umeno, Kyoichi Saito, K. Hagiwara, 2009 .

Effect of Salt Concentration of Cesium Solution on Cesium-Binding Capacity of Potassium Cobalt-Hexacyanoferrate-Impregnated Fiber

D. Umeno, Kyoichi Saito, T. Sugo, 2013 .

Protein-Binding Characteristics of Anion-Exchange Particles Prepared by Radiation-Induced Graft Polymerization at Low Temperatures

D. Umeno, Kyoichi Saito, N. Kubota, 2013 .

Robust and flexible platform for directed evolution of yeast genetic switches

A. Kondo, Jun Ishii, D. Umeno, 2021, Nature Communications.

Sequence-specific affinity precipitation of oligonucleotide using poly(N-isopropylacrylamide)-oligonucleotide conjugate.

M. Maeda, D. Umeno, T Mori, 2001, Biotechnology and bioengineering.

Labor-less, Automated Microfluidic Tandem Cell Processor for Visualizing Intracellular Molecules of Mammalian Cells.

D. Umeno, M. Yamada, M. Seki, 2019, Analytical chemistry.

Engineering proteins that bind, move, make and break DNA.

F. Arnold, C. Collins, Y. Yokobayashi, 2003, Current opinion in biotechnology.

Engineering proteins that bind, move, make and break DNA.

F. Arnold, C. Collins, Y. Yokobayashi, 2003, Current opinion in biotechnology.

Directed evolution of the stringency of the LuxR Vibrio fischeri quorum sensor without OFF-state selection.

D. Umeno, Kyoichi Saito, S. Kawai-Noma, 2020, ACS synthetic biology.

A system for the rapid determination of the mutation spectrum in Escherichia coli.

D. Umeno, Kyoichi Saito, Akinori Katabami, 2012, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

A nucleoside kinase as a dual selector for genetic switches and circuits

Kyoichi Saito, D. Umeno, Kyoichi Saito, 2010, Nucleic acids research.

Rapid and Liquid-Based Selection of Genetic Switches Using Nucleoside Kinase Fused with Aminoglycoside Phosphotransferase

D. Umeno, Kyoichi Saito, S. Kawai-Noma, 2015, PloS one.

Affinity Precipitation Separation of DNA Binding Protein Using Block Conjugate Composed of Poly(N-isopropylacrylamide) Grafted Double-Stranded DNA and Double-Stranded DNA Containing a Target Sequence

M. Maeda, D. Umeno, M. Murata, 2002, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

A highly selective biosynthetic pathway to non-natural C50 carotenoids assembled from moderately selective enzymes

T. Maoka, H. Hemmi, S. Takaichi, 2015, Nature Communications.

Impregnation structure of cobalt ferrocyanide microparticles by the polymer chain grafted onto nylon fiber

T. Kojima, D. Umeno, Kyoichi Saito, 2016 .

Cesium removal in freshwater using potassium cobalt hexacyanoferrate-impregnated fibers

T. Kojima, D. Umeno, Kyoichi Saito, 2014 .

Adsorption Isotherms for Cesium Ions in Seawater of Insoluble Cobalt and Nickel Ferrocyanide-Impregnated Fibers

D. Umeno, Kyoichi Saito, T. Sugo, 2014 .

A High-Throughput Colorimetric Screening Assay for Terpene Synthase Activity Based on Substrate Consumption

D. Umeno, Kyoichi Saito, Maiko Furubayashi, 2014, PloS one.

Pathway engineering for efficient biosynthesis of violaxanthin in Escherichia coli

M. Takemura, N. Misawa, T. Maoka, 2019, Applied Microbiology and Biotechnology.

Single stranded DNA-poly(N-isopropylacrylamide) conjugate for affinity precipitation separation of oligonucleotides

M. Maeda, D. Umeno, 1998 .

Construction of a pathway to C50-ε-carotene

T. Maoka, D. Umeno, Kyoichi Saito, 2019, PloS one.

Bacterial Production of Pinene by a Laboratory-Evolved Pinene-Synthase.

M. Ikeuchi, Yoko Iijima, D. Umeno, 2016, ACS synthetic biology.

Psoralen-containing vinyl monomer for conjugation of double-helical DNA with vinyl polymers.

M. Maeda, D. Umeno, C. Nishimura, 1994, Bioconjugate chemistry.

OFF-switching property of quorum sensor LuxR via As(III)-induced insoluble form.

D. Umeno, S. Kawai-Noma, R. Ayuba, 2022, Journal of bioscience and bioengineering.

Directed evolution and expression tuning of geraniol synthase for efficient geraniol production in Escherichia coli.

D. Umeno, Kyoichi Saito, A. Fujii, 2017, The Journal of general and applied microbiology.

Construction of a Nonnatural C60 Carotenoid Biosynthetic Pathway.

D. Umeno, Kyoichi Saito, Maiko Furubayashi, 2019, ACS synthetic biology.

Evolutionary analysis of the functional plasticity of Staphylococcus aureus C30 carotenoid synthase.

D. Umeno, Kyoichi Saito, Maiko Furubayashi, 2014, Journal of bioscience and bioengineering.

Removal of Urea from Water Using Urease-Immobilized Fibers

D. Umeno, Kyoichi Saito, K. Ando, 2013 .

Imprinting of Proteins on Poly mer-Coated DNA for Affinity Separation with Enhanced Selectivity

M. Maeda, D. Umeno, M. Kawasaki, 1998 .

Directed evolution of Vibrio fischeri LuxR signal sensitivity.

D. Umeno, Kyoichi Saito, S. Kawai-Noma, 2016, Journal of bioscience and bioengineering.

Directed evolution of the autoinducer selectivity of Vibrio fischeri LuxR.

D. Umeno, A. Tanaka, Kyoichi Saito, 2016, The Journal of general and applied microbiology.

Liquid-Based Iterative Recombineering Method Tolerant to Counter-Selection Escapes

I. Kawagishi, D. Umeno, Kyoichi Saito, 2015, PloS one.

Photolithographic coating of polymers on DNA as an approach for construction of nano-structures

M. Maeda, D. Umeno, M. Kawasaki, 1998 .

Poly(N-isopropylacrylamide) carrying double-stranded DNA for affinity separation of genotoxins

M. Maeda, D. Umeno, 1997 .

Diagnostic Polymer Reagents Carrying Dna

M. Maeda, Takeshi Mori, Y. Katayama, 2002 .

Synthesis and Characterization of Vinyl Polymer-Grafted DNA and Its AB-Type Block Conjugate with Native DNA Fragment

Mizuo Maeda, Masaharu Murata, M. Maeda, 1999 .

Nucleotide kinase-based selection system for genetic switches.

Kohei Ike, Daisuke Umeno, D. Umeno, 2014, Methods in molecular biology.

Propagation and Aggregation of Motile Cells of Escherichia coli Pattern

T. Tsujikawa, T. Sakurai, D. Umeno, 2018 .

Water-soluble conjugate of double-stranded DNA and Poly(N-isopropylacrylamide) for one-pot affinity precipitation separation of DNA-binding proteins.

M. Maeda, D. Umeno, M. Kawasaki, 1998, Bioconjugate chemistry.