S. Pfuhler

D. Schrenk, G. Lin, S. Pfuhler, 2020, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

Threshold of toxicological concern (TTC) for botanicals - Concentration data analysis of potentially genotoxic constituents to substantiate and extend the TTC approach to botanicals.

S. Barlow, S. Pfuhler, C. Mahony, 2020, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

AOP report: Development of an adverse outcome pathway for oxidative DNA damage leading to mutations and chromosomal aberrations

C. Yauk, G. Hendriks, S. Dertinger, 2022, Environmental and molecular mutagenesis.

A tiered approach to the use of alternatives to animal testing for the safety assessment of cosmetics: genotoxicity. A COLIPA analysis.

Nicola J Hewitt, Andreas Schepky, Kerstin Reisinger, 2010, Regulatory toxicology and pharmacology : RTP.

The BlueScreen HC assay to predict the genotoxic potential of fragrance materials

Gary M Williams, W. Dekant, H. Greim, 2022, Mutagenesis.

Validation of the 3D reconstructed human skin micronucleus (RSMN) assay: an animal-free alternative for following-up positive results from standard in vitro genotoxicity assays

M. Aardema, T. Downs, N. Hewitt, 2021, Mutagenesis.

Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward. Report of the working group from the 7th International Workshop on Genotoxicity Testing (IWGT).

T. Sofuni, S. Doak, J. van Benthem, 2020, Mutation research.

Role in a Testing Strategy

S. Pfuhler, K. Reisinger, 2017 .

Overview on Current Status

S. Pfuhler, K. Reisinger, 2017 .

Reconstructed Skin Micronucleus Assay (RSMN)

S. Pfuhler, K. Reisinger, 2017 .

Evaluation of aromatic amines in the EpiDerm™ 3D human reconstructed skin micronucleus (RSMN) assay

M. Aardema, S. Pfuhler, D. Holland, 2013 .

Evaluation of chemicals requiring metabolic activation in the EpiDerm™ 3D human reconstructed skin micronucleus (RSMN) assay.

M. Aardema, N. Hewitt, S. Pfuhler, 2013, Mutation research.

Common Considerations for Genotoxicity Assessment of Nanomaterials

S. Doak, M. Manjanatha, A. Collins, 2022, Frontiers in Toxicology.

Genotoxicty and mutagenicity.

M. Aardema, D. Maurici, C. Krul, 2005, Alternatives to laboratory animals : ATLA.

Novel Insights into Pyrrolizidine Alkaloid Toxicity and Implications for Risk Assessment: Occurrence, Genotoxicity, Toxicokinetics, Risk Assessment–AWorkshop Report

D. Schrenk, G. Lin, S. Pfuhler, 2021 .

Novel Insights into Pyrrolizidine Alkaloid Toxicity and Implications for Risk Assessment: Occurrence, Genotoxicity, Toxicokinetics, Risk Assessment–A Workshop Report

D. Schrenk, G. Lin, S. Pfuhler, 2021, Planta Medica.

Relative potency of fifteen pyrrolizidine alkaloids to induce DNA damage as measured by micronucleus induction in HepaRG human liver cells.

S. Pfuhler, C. Mahony, C. Lester, 2018, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

A weight of evidence review of the genotoxicity of titanium dioxide (TiO₂).

Helinor J Johnston, M. Aardema, E. Donner, 2022, Regulatory toxicology and pharmacology : RTP.

Metabolism of oxidative hair dyes: An overview of in vitro, in vivo, and clinical studies

M. Aardema, T. Hu, S. Pfuhler, 2007 .

Genetic toxicity assessment: employing the best science for human safety evaluation part IV: Recommendation of a working group of the Gesellschaft fuer Umwelt-Mutationsforschung (GUM) for a simple and straightforward approach to genotoxicity testing.

Albrecht Poth, Silvio Albertini, Stefan Pfuhler, 2007, Toxicological sciences : an official journal of the Society of Toxicology.

Measuring DNA modifications with the comet assay: a compendium of protocols

I. Kooter, G. Brunborg, Diana Anderson, 2023, Nature Protocols.

Assessment of mechanisms driving non-linear dose-response relationships in genotoxicity testing.

A. Sutter, A. Elhajouji, B. Kaina, 2015, Mutation research. Reviews in mutation research.

Utility of a next generation framework for assessment of genomic damage: A case study using the industrial chemical benzene

Mirjam Luijten, Federica Madia, Jan van Benthem, 2019, Environmental and molecular mutagenesis.

In Vitro and Integrated In Vivo Strategies to Reduce Animal Use in Genotoxicity Testing.

S. Doak, R. Corvi, S. Pfuhler, 2021, Mutagenesis.

The Cosmetics Europe strategy for animal-free genotoxicity testing: project status up-date.

R Fautz, J Barroso, G. Ouedraogo, 2014, Toxicology in Vitro.

Effect of 2-acetylaminofluorene and its genotoxic metabolites on DNA adduct formation and DNA damage in 3D reconstructed human skin tissue models

V. Arlt, T. Downs, S. Pfuhler, 2019, Mutagenesis.

Reduction of use of animals in regulatory genotoxicity testing: Identification and implementation opportunities-Report from an ECVAM workshop.

Raffaella Corvi, Peter Kasper, Cyrille Krul, 2009, Mutation research.

Validation of the 3D Skin Comet assay using full thickness skin models: Transferability and reproducibility.

Markus Schulz, Andreas Luch, Sebastian Hoffmann, 2018, Mutation research. Genetic toxicology and environmental mutagenesis.

Reduction of misleading ("false") positive results in mammalian cell genotoxicity assays. I. Choice of cell type.

Katie Smith, David Kirkland, Stefan Pfuhler, 2012, Mutation research.

Reduction of misleading ("false") positive results in mammalian cell genotoxicity assays. II. Importance of accurate toxicity measurement.

Katie Smith, David Kirkland, Stefan Pfuhler, 2012, Mutation research.

Relationships between p53 status, apoptosis and induction of micronuclei in different human and mouse cell lines in vitro: Implications for improving existing assays.

E. Lorge, J. Tanir, D. Kirkland, 2015, Mutation research. Genetic toxicology and environmental mutagenesis.

ECVAM retrospective validation of in vitro micronucleus test (MNT)

Raffaella Corvi, Sebastian Hoffmann, Thomas Hartung, 2008, Mutagenesis.

3.7. Genotoxicity and Mutagenicity

Raffaella Corvi, Barry Phillips, Cyrille Krul, 2005 .

Application of the adverse outcome pathway framework to genotoxic modes of action

A. Fornace, A. Elhajouji, C. Yauk, 2019, Environmental and molecular mutagenesis.

The rat bone marrow micronucleus test: Statistical considerations on historical negative control data

Roland Frötschl, Volker Harm, Rupert Kellner, 2019, Regulatory toxicology and pharmacology : RTP.

N-acetylation of three aromatic amine hair dye precursor molecules eliminates their genotoxic potential.

S. Pfuhler, A. Zeller, 2014, Mutagenesis.

Use of human in vitro skin models for accurate and ethical risk assessment: metabolic considerations.

Joan Eilstein, Nicola J Hewitt, Kerstin Reisinger, 2013, Toxicological sciences : an official journal of the Society of Toxicology.

Reduction of misleading ("false") positive results in mammalian cell genotoxicity assays. III: sensitivity of human cell types to known genotoxic agents.

Katie Smith, David Kirkland, Stefan Pfuhler, 2014, Mutation research. Genetic toxicology and environmental mutagenesis.

How to reduce false positive results when undertaking in vitro genotoxicity testing and thus avoid unnecessary follow-up animal tests: Report of an ECVAM Workshop.

Raffaella Corvi, Elizabeth Parry, Lutz Müller, 2007, Mutation research.

Critical issues with the in vivo comet assay: A report of the comet assay working group in the 6th International Workshop on Genotoxicity Testing (IWGT).

Hajime Kojima, Peter Kasper, Yoshifumi Uno, 2015, Mutation research. Genetic toxicology and environmental mutagenesis.

Summary of major conclusions from the 7th International Workshop on Genotoxicity Testing (IWGT), Tokyo, Japan.

C. Yauk, F. Marchetti, M. Honma, 2020, Mutation research.

In vitro genotoxicity test approaches with better predictivity: summary of an IWGT workshop.

A. Elhajouji, J. van Benthem, R. Corvi, 2011, Mutation research.

The reconstructed skin micronucleus assay (RSMN) in EpiDerm™: detailed protocol and harmonized scoring atlas.

M. Aardema, N. Hewitt, G. Ouedraogo, 2011, Mutation research.

Comet assay in reconstructed 3D human epidermal skin models—investigation of intra- and inter-laboratory reproducibility with coded chemicals

Raffaella Corvi, Kerstin Reisinger, Andreas Zeller, 2013, Mutagenesis.

A comparison of transgenic rodent mutation and in vivo comet assay responses for 91 chemicals.

M. LeBaron, K. Witt, M. Aardema, 2019, Mutation research. Genetic toxicology and environmental mutagenesis.

Assessing the genotoxicity and carcinogenicity of 2-chloroethanol through structure activity relationships and in vitro testing approaches.

S. Pfuhler, Thomas Roth, Henrike Peuschel, 2022, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

Emerging approaches in predictive toxicology

Ronald D Snyder, Nigel Greene, Luoping Zhang, 2014, Environmental and molecular mutagenesis.

Silica nanoparticles administered at the maximum tolerated dose induce genotoxic effects through an inflammatory reaction while gold nanoparticles do not.

Stefan Pfuhler, Thomas R Downs, M. Wagner, 2012, Mutation research.

Risk Governance of Emerging Technologies Demonstrated in Terms of its Applicability to Nanomaterials.

Igor Linkov, Finbarr Murphy, Alena Bartonova, 2020, Small.

Genotoxicity of nanomaterials: Refining strategies and tests for hazard identification

Robert Landsiedel, Masamitsu Honma, Shareen H Doak, 2013, Environmental and molecular mutagenesis.

The in vitro genotoxicity potency of mixtures of pyrrolizidine alkaloids can be explained by dose addition of the individual mixture components

S. Pfuhler, C. Mahony, Ashley Allemang, 2022, Environmental and molecular mutagenesis.

The 3D reconstructed skin micronucleus assay using imaging flow cytometry and deep learning: A proof-of-principle investigation.

S. Pfuhler, R. DeMarco, Ashley Allemang, 2021, Mutation research.

Weak silica nanomaterial-induced genotoxicity can be explained by indirect DNA damage as shown by the OGG1-modified comet assay and genomic analysis

T. Downs, S. Pfuhler, Yuching Shan, 2017, Mutagenesis.

Quantitative in vitro to in vivo extrapolation of genotoxicity data provides protective estimates of in vivo dose

Marc A. Beal, G. Hendriks, G. Johnson, 2022, Environmental and molecular mutagenesis.

Carcinogenicity.

M. Aardema, D. Maurici, C. Krul, 2019, Alternatives to laboratory animals : ATLA.

3.10. Carcinogenicity

Raffaella Corvi, Barry Phillips, Cyrille Krul, 2005, Alternatives to laboratory animals : ATLA.

Establishment of timetables for the phasing out of animal experiments for cosmetics Carcinogenicity

M. Aardema, D. Maurici, C. Krul, 2004 .

Emerging approaches in predictive toxicology

N. Greene, M. Aardema, Luoping Zhang, 2014, Environmental and molecular mutagenesis.

Use of the EpiDermTM 3D reconstructed skin micronucleus assay for fragrance materials

A. M. Api, M. Aardema, S. Pfuhler, 2021, Mutagenesis.

Validation of the 3D Skin Comet assay using full thickness skin models: Transferability and reproducibility.

A. Luch, C. Krul, T. Downs, 2018, Mutation research. Genetic toxicology and environmental mutagenesis.

Automation and validation of micronucleus detection in the 3D EpiDerm™ human reconstructed skin assay and correlation with 2D dose responses

S. Doak, G. Jenkins, S. Pfuhler, 2014, Mutagenesis.

The Cosmetics Europe strategy for animal-free genotoxicity testing: project status up-date.

G. Ouedraogo, S. Pfuhler, K. Reisinger, 2014, Toxicology in Vitro.

A tiered approach to the use of alternatives to animal testing for the safety assessment of cosmetics: genotoxicity. A COLIPA analysis.

M. Aardema, P. Carmichael, N. Hewitt, 2010, Regulatory toxicology and pharmacology : RTP.

Utility of ToxTracker in animal alternative testing strategy for fragrance materials

A. M. Api, G. Hendriks, S. Pfuhler, 2023, Environmental and molecular mutagenesis.

Mutation Research/Genetic Toxicology and Environmental Mutagenesis

J. van Benthem, F. Madia, R. Corvi, 2009 .

In vivo Mammalian Alkaline Comet Assay: Method Adapted for Genotoxicity Assessment of Nanomaterials

M. Manjanatha, A. Collins, S. Pfuhler, 2022, Frontiers in Toxicology.

A comparison of transgenic rodent mutation and in vivo comet assay responses for 91 chemicals.

M. LeBaron, K. Witt, M. Aardema, 2019, Mutation research. Genetic toxicology and environmental mutagenesis.

S. Albertini, A. Poth, S. Pfuhler, 2007, Toxicological sciences : an official journal of the Society of Toxicology.

Interlaboratory validation of the ToxTracker assay: An in vitro reporter assay for mechanistic genotoxicity assessment

G. Hendriks, J. van Benthem, S. Pfuhler, 2024, Environmental and molecular mutagenesis.

A comparison of classical and 21st century genotoxicity tools: A proof of concept study of 18 chemicals comparing in vitro micronucleus, ToxTracker and genomics‐based methods (TGx‐DDI, whole genome clustering and connectivity mapping)

S. Pfuhler, J. Krailler, Ashley Allemang, 2020, Environmental and molecular mutagenesis.

In vivo alkaline comet assay: Statistical considerations on historical negative and positive control data.

S. Pfuhler, Marie Z. Vasquez, R. Frötschl, 2024, Regulatory toxicology and pharmacology : RTP.