T. Aoki

发表

Hydratase activities of green fluorescent protein tagged human multifunctional enzyme type 2 hydratase domain and its variants.

Shirou Tsuchida, Koutarou Kawamoto, N. Endo, 2012, Journal of oleo science.

Fluorescence Resonance Energy Transfer-Based Assay for DNA-Binding Protein Tagged by Green Fluorescent Protein

Shirou Tsuchida, T. Aoki, T. Imamura, 2006, Bioscience, biotechnology, and biochemistry.

A Flexible Single-Step Detection of Blotted Antigen Using a Fusion Protein between Protein A and Green Fluorescent Protein

T. Aoki, H. Watabe, M. Miyashita, 2000, Bioscience, biotechnology, and biochemistry.

Construction of a fusion protein between protein A and green fluorescent protein and its application to Western blotting

H. Leffert, T. Aoki, H. Watabe, 1996, FEBS letters.

A new method for detecting single nucleotide polymorphism using GFP-display.

T. Aoki, T. Imamura, H. Watabe, 2004, Journal of biochemical and biophysical methods.

Effect of extracellular calcium on the gene expression of bone morphogenetic protein-2 and -4 of normal human bone cells

O. Nakade, T. Kaku, T. Aoki, 2001, Journal of Bone and Mineral Metabolism.

A simple method for preparing a diamino cellulose disk from a dialdehyde cellulose disk by reductive amination using 2-picoline-borane

Shirou Tsuchida, N. Hamaue, T. Aoki, 2022, Cellulose.

Purification of recombinant human pepsinogens and their application as immunoassay standards

T. Aoki, H. Watabe, H. Onagi, 1998, Biochemistry and molecular biology international.

A Highly Sensitive Assay for Proteases Using Staphylococcal Protein A Fused with Enhanced Green Fluorescent Protein

T. Aoki, H. Watabe, H. Fujino, 2002, Bioscience, biotechnology, and biochemistry.

Chiral separation, determination of absolute configuration, and high-performance liquid chromatography detection of enantiomeric 3-hydroxyhexadecanoyl-CoA.

Shirou Tsuchida, Koutarou Kawamoto, K. Nunome, 2011, Journal of oleo science.

Discordant expression and variable numbers of neighboring GGA- and GAA-rich triplet repeats in the 3' untranslated regions of two groups of messenger RNAs encoded by the rat polymeric immunoglobulin receptor gene.

H. Leffert, A. Gleiberman, T. Aoki, 1995, Nucleic acids research.

Functional pleiotropy of an intramolecular triplex-forming fragment from the 3'-UTR of the rat Pigr gene.

J. R. Fresco, C. Schildkraut, I. Fabregat, 2001, Physiological genomics.

Attenuation of gene expression by a trinucleotide repeat-rich tract from the terminal exon of the rat hepatic polymeric immunoglobulin receptor gene.

H. Leffert, T. Aoki, K. Koch, 1997, Journal of molecular biology.

Site-specific integration of targeted DNA into animal cell genomes.

Y. Wang, H. Leffert, A. Gleiberman, 2000, Gene.

Application of “Homogeneous Assay for Fluorescence Concentrated on Membrane” to the Analysis of the Substrate Specificity of Protease

Shirou Tsuchida, K. Nunome, N. Hamaue, 2010, Bioscience, biotechnology, and biochemistry.

Screening of recombinant Escherichia coli using activation of green fluorescent protein as an indicator.

Shirou Tsuchida, N. Hamaue, T. Aoki, 2014, Biochemical and biophysical research communications.

Verification of the guidelines for lupus anticoagulant detection: usefulness of index for circulating anticoagulant in APTT mixing test.

T. Aoki, M. Ieko, S. Naito, 2014, Thrombosis research.

Index of circulating anticoagulant cut‐off value establishment in activated partial thromboplastin time mixing test for lupus anticoagulant diagnosis

T. Aoki, S. Naito, O. Kumano, 2013, Journal of thrombosis and haemostasis : JTH.

Conversion of cathepsin E to enzymatic unstable form in gastric cancer cells.

T. Aoki, H. Watabe, T. Furukawa, 1995, Biological & pharmaceutical bulletin.

Entacapone, a catechol-O-methyltransferase inhibitor, improves the motor activity and dopamine content of basal ganglia in a rat model of Parkinson's disease induced by Japanese encephalitis virus

M. Hirafuji, Shirou Tsuchida, N. Hamaue, 2010, Brain Research.

Effect of sequential C‐terminal tryptophans on green fluorescent protein fluorescence

Shirou Tsuchida, N. Hamaue, T. Aoki, 2018, FEBS open bio.

Synthesis and Detection by HPLC of 3-Oxohexadecanoyl-CoA for the Study of Peroxisomal Bifunctional Proteins.

Shirou Tsuchida, N. Hamaue, T. Aoki, 2017, Journal of oleo science.

Analysis of enoyl-coenzyme A hydratase activity and its stereospecificity using high-performance liquid chromatography equipped with chiral separation column.

Shirou Tsuchida, Koutarou Kawamoto, K. Nunome, 2011, Journal of oleo science.

Effects of naturally occurring missense mutations and G525V in the hydratase domain of human d-bifunctional protein on hydratase activity

Shirou Tsuchida, N. Hamaue, T. Aoki, 2014, Molecular genetics and metabolism reports.

A homogeneous assay for relative affinity of binding proteins using a green fluorescent protein tag and membrane disk.

T. Aoki, H. Watabe, M. Satoh, 2005, Analytical biochemistry.

A simple and rapid immunoassay system using green fluorescent protein tag.

H. Leffert, T. Aoki, H. Watabe, 1997, Journal of immunoassay.

General properties of GFP-display, an electrophoretic analysis for single amino acid changes in target polypeptides.

T. Aoki, H. Watabe, H. Fujino, 2003, Analytical biochemistry.

"GFP-display," an easy detection method for single amino acid changes in a target polypeptide: application to random mutagenesis.

T. Aoki, H. Watabe, H. Fujino, 2002, Analytical biochemistry.

A new approach to gene mutation analysis using "GFP-Display".

T. Aoki, H. Watabe, H. Fujino, 2000, Journal of biochemistry.

Application of green fluorescent protein-protein A fusion protein to western blotting.

H. Leffert, T. Aoki, H. Watabe, 1999, Methods in enzymology.