I. Matsumura

发表

Vectors for the Generation of FLAG-or EGFP-Tagged cDNA Constructs and EGFP-Tagged Antisense RNA Constructs

I. Matsumura, 2022 .

Whole plasmid mutagenic PCR for directed protein evolution.

Ichiro Matsumura, I. Matsumura, Lori A Rowe, 2005, Biomolecular engineering.

Pooling for Improved Screening of Combinatorial Libraries for Directed Evolution

Jay H. Lee, Cody U Spencer, M. Realff, 2006, Biotechnology progress.

A study in molecular contingency: glutamine phosphoribosylpyrophosphate amidotransferase is a promiscuous and evolvable phosphoribosylanthranilate isomerase.

Ichiro Matsumura, Wayne M. Patrick, W. Patrick, 2008, Journal of molecular biology.

Directed Evolution of Aminoglycoside Phosphotransferase (3′) Type IIIa Variants That Inactivate Amikacin but Impose Significant Fitness Costs

I. Matsumura, Joseph R. Kramer, 2013, PloS one.

Why Johnny can't clone: Common pitfalls and not so common solutions.

I. Matsumura, 2015, BioTechniques.

Dan Tawfik’s Lessons for Protein Engineers about Enzymes Adapting to New Substrates

W. Patrick, I. Matsumura, W. M. Patrick, 2022, Biochemistry.

Rational Design of a Plasmid Origin That Replicates Efficiently in Both Gram-Positive and Gram-Negative Bacteria

I. Matsumura, A. Bryksin, 2010, PloS one.

A quarter century of reaping what we SOE.

I. Matsumura, 2013, BioTechniques.

Transformable facultative thermophile Geobacillus stearothermophilus NUB3621 as a host strain for metabolic engineering

I. Matsumura, S. Robic, K. Blanchard, 2014, Applied Microbiology and Biotechnology.

Aptamers as therapeutic and diagnostic reagents: problems and prospects.

A D Ellington, A. Ellington, I. Matsumura, 1997, Current opinion in chemical biology.

Overlap extension PCR cloning.

I. Matsumura, A. Bryksin, 2013, Methods in molecular biology.

Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of beta-fucosidase from beta-galactosidase.

I. Matsumura, Monal R Parikh, 2005, Journal of molecular biology.

Engineering DNA and RNA Regulatory Regions through Random Mutagenesis and Screening

J. Gallivan, I. Matsumura, Sean A. Lynch, 2009 .

Rapid Evolution of β-Glucuronidase Specificity by Saturation Mutagenesis of an Active Site Loop*

Ichiro Matsumura, Melissa L Geddie, I. Matsumura, 2004, Journal of Biological Chemistry.

The identification of an Onchocerca-specific recombinant antigen containing a T cell epitope.

F. Perler, T. Nutman, I. Matsumura, 1990, Journal of immunology.

Diversification and specialization of HIV protease function during in vitro evolution.

I. Matsumura, D. Greene, Taryn L O'Loughlin, 2006, Molecular biology and evolution.

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids.

Ichiro Matsumura, Anton V Bryksin, I. Matsumura, 2010, BioTechniques.

Bacterial Cells as Model Factories

Ichiro Matsumura, I. Matsumura, 2013 .

A comparison of directed evolution approaches using the beta-glucuronidase model system.

Ichiro Matsumura, Melissa L Geddie, I. Matsumura, 2003, Journal of molecular biology.

In vitro evolution of thermostable p53 variants

A. Ellington, I. Matsumura, 2008, Protein science : a publication of the Protein Society.

Multicopy suppression underpins metabolic evolvability.

Wayne M Patrick, Ichiro Matsumura, Erik M. Quandt, 2007, Molecular biology and evolution.

Substrate ambiguous enzymes within the Escherichia coli proteome offer different evolutionary solutions to the same problem.

I. Matsumura, S. Yip, 2013, Molecular biology and evolution.

Artificially evolved Synechococcus PCC6301 Rubisco variants exhibit improvements in folding and catalytic efficiency.

I. Matsumura, S. Whitney, D. Greene, 2007, The Biochemical journal.

In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates.

A D Ellington, A. Ellington, I. Matsumura, 2001, Journal of molecular biology.

Directed evolution of the surface chemistry of the reporter enzyme β-glucuronidase

Andrew D. Ellington, Peter D. Vize, John B. Wallingford, 1999, Nature Biotechnology.

Methylase-assisted subcloning for high throughput BioBrick assembly

Ichiro Matsumura, I. Matsumura, 2020, PeerJ.

Semi-automated Tip Snip cloning of restriction fragments into and out of plasmid polylinkers.

I. Matsumura, 2017, BioTechniques.

Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli.

Ichiro Matsumura, I. Matsumura, Monal R Parikh, 2006, Protein engineering, design & selection : PEDS.

High throughput microplate screens for directed protein evolution.

Ichiro Matsumura, Melissa L Geddie, I. Matsumura, 2004, Methods in enzymology.

Evidence for a new family of evolutionarily conserved homeobox genes.

D. Weisblat, I. Matsumura, I Matsumura, 1990, Nucleic acids research.

Escherichia coli Deletion Mutants Illuminate Trade-Offs between Growth Rate and Flux through a Foreign Anabolic Pathway

I. Matsumura, A. Bryksin, Kelly C. Falls, 2014, PloS one.

Antibody-induced oligomerization and activation of an engineered reporter enzyme.

I. Matsumura, Melissa L. Geddie, 2007, Journal of molecular biology.

The adenosine deaminases of Plasmodium vivax and Plasmodium falciparum exhibit surprising differences in ligand specificity.

A. Ivanov, I. Matsumura, 2012, Journal of molecular graphics & modelling.

Expression Vectors for Acinetobacter baylyi ADP1

C. D. Murin, I. Matsumura, A. Bryksin, 2011, Applied and Environmental Microbiology.

Mutagenic polymerase chain reaction of protein-coding genes for in vitro evolution.

A. Ellington, I. Matsumura, 2002, Methods in molecular biology.

Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases.

I. Matsumura, 2022, BioTechniques.

Natural history as a predictor of protein evolvability.

W. Patrick, I. Matsumura, Taryn L O'Loughlin, 2006, Protein engineering, design & selection : PEDS.

Submitted as a Research Article to Molecular Biology and Evolution.

W. Patrick, I. Matsumura, Erik M Quandt, 2007 .

Optimization of heterologous gene expression for in vitro evolution.

A D Ellington, A. Ellington, I. Matsumura, 2001, BioTechniques.

Rational Design of p53, an Intrinsically Unstructured Protein, for the Fabrication of Novel Molecular Sensors*

I. Matsumura, K. Woods, Melissa L. Geddie, 2005, Journal of Biological Chemistry.

HIV protease-activated molecular switches based on beta-glucuronidase and alkaline phosphatase.

I. Matsumura, Taryn L O'Loughlin, 2006, Combinatorial chemistry & high throughput screening.

Material For : Overlap extension PCR cloning : a simple and reliable way to create recombinant plasmids

I. Matsumura, A. Bryksin, 2022 .

Statistical noise from recombinant plasmids can be abated via complementation of a ribosomal protein gene deletion.

I. Matsumura, D. Chyong, 2019, Protein engineering, design & selection : PEDS.

Simulation Modeling of Pooling for Combinatorial Protein Engineering

Jay H. Lee, Anshul Dubey, Karen M Polizzi, 2005, Journal of biomolecular screening.

Transformable facultative thermophile Geobacillus stearothermophilus NUB3621 as a host strain for metabolic engineering

I. Matsumura, S. Robic, K. Blanchard, 2014, Applied Microbiology and Biotechnology.