R. Akada

发表

A Novel Terminator Primer and Enhancer Reagents for Direct Expression of PCR-Amplified Genes in Mammalian Cells

Mikiko Nakamura, Ayako Suzuki, J. Akada, 2015, Molecular Biotechnology.

High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast?

H. Hoshida, R. Akada, B. M. Abdel-Banat, 2009, Applied Microbiology and Biotechnology.

Whole-Genome Sequencing of Sake Yeast Saccharomyces cerevisiae Kyokai no. 7

Akira Nishimura, Katsuhiko Kitamoto, Yoshiharu Inoue, 2011, DNA research : an international journal for rapid publication of reports on genes and genomes.

Positive and negative elements upstream of the meiosis-specific glucoamylase gene in Saccharomyces cerevisiae.

R. Akada, I. Yamashita, M. Nakamura, 1991, Molecular & general genetics : MGG.

Positive and negative elements upstream of the meiosis-specific glucoamylase gene in Saccbaromyces cerevisiae

Motonao Nakamura, R. Akada, I. Yamashita, 1991, Molecular and General Genetics MGG.

Autophagy regulates levels of tumor suppressor enzyme protein phosphatase 6

M. Oshima, T. Usui, H. Hoshida, 2020, Cancer science.

Construction of Flocculent Kluyveromyces marxianus Strains Suitable for High-Temperature Ethanol Fermentation

H. Hoshida, R. Akada, B. M. Abdel-Banat, 2009, Bioscience, biotechnology, and biochemistry.

Structure-based Analyses Reveal Distinct Binding Sites for Atg2 and Phosphoinositides in Atg18*

F. Inagaki, N. Noda, Y. Ohsumi, 2012, The Journal of Biological Chemistry.

Isolation of five laccase gene sequences from the white-rot fungus Trametes sanguinea by PCR, and cloning, characterization and expression of the laccase cDNA in yeasts.

R. Akada, M. Nakao, Y. Nishizawa, 2001, Journal of bioscience and bioengineering.

Role of soluble factors (gamones) and calcium ions in conjugation of the ciliate Euplotes patella syngen 2

R. Akada, 1985 .

Genetically modified industrial yeast ready for application.

R. Akada, 2002, Journal of bioscience and bioengineering.

Anoxia-induced mitophagy in the yeast Kluyveromyces marxianus.

H. Hoshida, R. Akada, Shota Kagawa, 2020, FEMS yeast research.

Multiple genes coding for precursors of rhodotorucine A, a farnesyl peptide mating pheromone of the basidiomycetous yeast Rhodosporidium toruloides

R. Akada, I. Yamashita, S. Fukui, 1989, Molecular and cellular biology.

Genomic organization of multiple genes coding for rhodotorucine A, a lipopeptide mating pheromone of the basidiomycetous yeast Rhodosporidium toruloides

R. Akada, I. Yamashita, S. Fukui, 1989, Archives of Microbiology.

Loss of heterozygosity is induced in Candida albicans by ultraviolet irradiation

R. Akada, H. Kumagai, Kenji Yamamoto, 2008, Applied Microbiology and Biotechnology.

Morphological Changes of Mitochondria and Actin Cytoskeleton in the Yeast Saccharomyces cerevisiae During Diauxic Growth and Glucose Depletion Culture

H. Hoshida, R. Akada, I. Miyakawa, 2022, CYTOLOGIA.

Cloning of a Gene Coding for Rhodotorucine A,a Farnesyl Peptide Mating Pheromone of Rhodosporidium toruloides

R. Akada, I. Yamashita, S. Fukui, 1987 .

Construction of recombinant sake yeast containing a dominant FAS2 mutation without extraneous sequences by a two-step gene replacement protocol.

R. Akada, Y. Nishizawa, K. Matsuo, 1999, Journal of bioscience and bioengineering.

Inhibition of Distinct Proline- or N-Acetylglucosamine-Induced Hyphal Formation Pathways by Proline Analogs in Candida albicans

H. Hoshida, R. Akada, Tatsuki Sato, 2020, BioMed research international.

Identification of essential intron sequences that enhance gene expression independently of splicing in the yeast Saccharomyces cerevisiae.

H. Hoshida, R. Akada, S. Goto, 2022, Biochimica et biophysica acta. Gene regulatory mechanisms.

Direct mating between diploid sake strains of Saccharomyces cerevisiae

H. Hoshida, R. Akada, Y. Nishizawa, 2006, Applied Microbiology and Biotechnology.

APPLIED GENETICS AND MOLECULAR BIOTECHNOLOGY

H. Hoshida, R. Akada, Y. Nishizawa, 2006 .

Self-cloning Yeast Strains Containing Novel FAS2 Mutations Produce a Higher Amount of Ethyl Caproate in Japanese Sake

H. Hoshida, R. Akada, Y. Nishizawa, 2004, Bioscience, biotechnology, and biochemistry.

Identification and Mutational Analysis of Escherichia coli Sorbitol-Enhanced Glucose-Repressed srlA Promoter Expressed in LB Medium by Using Homologous Recombination and One-Round PCR Products

Mikiko Nakamura, H. Hoshida, R. Akada, 2018, Molecular Biotechnology.

N-glycosylation deficiency enhanced heterologous production of a Bacillus licheniformis thermostable α-amylase in Saccharomyces cerevisiae

H. Hoshida, R. Akada, Tsuneyasu Fujita, 2013, Applied Microbiology and Biotechnology.

End Joining-Mediated Gene Expression in Mammalian Cells Using PCR-Amplified DNA Constructs that Contain Terminator in Front of Promoter

Mikiko Nakamura, Ayako Suzuki, J. Akada, 2015, Molecular Biotechnology.

High-Temperature Ethanol Fermentation and Transformation with Linear DNA in the Thermotolerant Yeast Kluyveromyces marxianus DMKU3-1042

M. Yamada, H. Hoshida, R. Akada, 2008, Applied and Environmental Microbiology.

Upstream regions of the yeast glucoamylase gene which are required for efficient transcription

M. Inui, R. Akada, I. Yamashita, 1989 .

1 Polymorphisms in the LAC 12 gene explain lactose utilisation 1 variability in Kluyveromyces marxianus strains 2 3

E. Boles, J. Morrissey, R. Akada, 2019 .

Polymorphisms in the LAC12 gene explain lactose utilisation variability in Kluyveromyces marxianus strains

E. Boles, J. Morrissey, H. Hoshida, 2017, FEMS yeast research.

TheSaccharomyces cerevisiae genes (CMP1 andCMP2) encoding calmodulin-binding proteins homologous to the catalytic subunit of mammalian protein phosphatase 2B

R. Akada, S. Fukui, T. Miyakawa, 1991, Molecular and General Genetics MGG.

Genomic analyses of thermotolerant microorganisms used for high-temperature fermentations

Mamoru Yamada, Rinji Akada, Tomoyuki Kosaka, 2016, Bioscience, biotechnology, and biochemistry.

Molecular Mechanisms of Thermotolerance of Thermotolerant Fermentation Microorganisms

M. Yamada, H. Hoshida, R. Akada, 2015 .

Isolation of Auxotrophic Mutants of Diploid Industrial Yeast Strains after UV Mutagenesis

H. Hoshida, R. Akada, Y. Nishizawa, 2005, Applied and Environmental Microbiology.

Construction of a self-cloning sake yeast that overexpresses alcohol acetyltransferase gene by a two-step gene replacement protocol

R. Akada, Y. Nishizawa, H. Hoshida, 2004, Applied Microbiology and Biotechnology.

Sets of integrating plasmids and gene disruption cassettes containing improved counter‐selection markers designed for repeated use in budding yeast

H. Hoshida, R. Akada, Y. Nishizawa, 2002, Yeast.

Detection of a point mutation in FAS2 gene of sake yeast strains by allele-specific PCR amplification.

R. Akada, Y. Nishizawa, H. Hoshida, 2001, Journal of bioscience and bioengineering.

Copper-Dependent Production of a Pycnoporus coccineus Extracellular Laccase in Aspergillus oryzae and Saccharomyces cerevisiae

H. Hoshida, R. Akada, K. Murata, 2005, Bioscience, biotechnology, and biochemistry.

Relationships between cell cycle and conjugation in the ciliate Euplotes patella syngen 2

R. Akada, 1985 .

Structural insights into Atg10-mediated formation of the autophagy-essential Atg12-Atg5 conjugate.

F. Inagaki, N. Noda, Y. Ohsumi, 2012, Structure.

Structural basis of starvation-induced assembly of the autophagy initiation complex

Yayoi Kimura, Hisashi Hirano, Fuyuhiko Inagaki, 2014, Nature Structural &Molecular Biology.

Gene expression analysis using strains constructed by NHEJ-mediated one-step promoter cloning in the yeast Kluyveromyces marxianus.

H. Fujii, Ayako Suzuki, H. Hoshida, 2015, FEMS yeast research.

Random and targeted gene integrations through the control of non‐homologous end joining in the yeast Kluyveromyces marxianus

H. Hoshida, R. Akada, Sanom Nonklang, 2009, Yeast.

Mutant screening for oncogenes of Ewing’s sarcoma using yeast

Mikiko Nakamura, J. Akada, H. Hoshida, 2015, Applied Microbiology and Biotechnology.

Isolation of a novel mutant strain of Saccharomyces cerevisiae by an ethyl methane sulfonate-induced mutagenesis approach as a high producer of bioethanol.

K. Ghaedi, H. Zarkesh-Esfahani, R. Akada, 2008, Journal of bioscience and bioengineering.

Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae

H. Hoshida, R. Akada, Y. Nishizawa, 2002, Yeast.

Involvement of Ca2+/Calmodulin in Sexual Differentiation Induced by Mating Pheromone Rhodotorucine A in Rhodosporidium toruloides

R. Akada, S. Fukui, T. Miyakawa, 1986 .

Initiation of meiosis and sporulation in Saccharomyces cerevisiae requires a novel protein kinase homologue

R. Akada, K. Kominami, Y. Sakata, 1990, Molecular and General Genetics MGG.

Screening and identification of yeast sequences that cause growth inhibition when overexpressed

R. Akada, I. Yamashita, J. Yamamoto, 1997, Molecular and General Genetics MGG.

Accumulation of eicosapentaenoic acid in Nannochloropsis sp. in response to elevated CO2 concentrations

H. Hoshida, R. Akada, A. Minematsu, 2005, Journal of Applied Phycology.

Novel Small-Molecule Compounds That Affect Cellular Morphogenesis in Yeast and Mammalian Cells

Mikiko Nakamura, H. Hoshida, R. Akada, 2013, Bioscience, biotechnology, and biochemistry.

The Thermotolerant Yeast Kluyveromyces marxianus Is a Useful Organism for Structural and Biochemical Studies of Autophagy*

T. Itoh, F. Inagaki, N. Noda, 2015, The Journal of Biological Chemistry.

Helicobacter pylori CagA inhibits endocytosis of cytotoxin VacA in host cells

T. Hirayama, M. Matsuzaki, S. Terai, 2010, Disease Models & Mechanisms.

Triple gene expressions in yeast, Escherichia coli, and mammalian cells by transferring DNA fragments amplified from a mother yeast expression plasmid.

Mikiko Nakamura, Ayako Suzuki, H. Hoshida, 2022, Journal of bioscience and bioengineering.

Various short autonomously replicating sequences from the yeast Kluyveromyces marxianus seemingly without canonical consensus

H. Hoshida, R. Akada, B. M. Abdel-Banat, 2021, Current research in microbial sciences.

YHp as a highly stable, hyper‐copy, hyper‐expression plasmid constructed using a full 2‐μm circle sequence in cir0 strains of Saccharomyces cerevisiae

Mikiko Nakamura, H. Hoshida, R. Akada, 2019, Yeast.

5´-UTR introns enhance protein expression in the yeast Saccharomyces cerevisiae

Tohru Yarimizu, H. Hoshida, R. Akada, 2016, Applied Microbiology and Biotechnology.

Screening of accurate clones for gene synthesis in yeast.

Mikiko Nakamura, Tohru Yarimizu, H. Hoshida, 2015, Journal of bioscience and bioengineering.

Non‐homologous end joining‐mediated functional marker selection for DNA cloning in the yeast Kluyveromyces marxianus

Mikiko Nakamura, Ayako Suzuki, H. Hoshida, 2014, Yeast.

Identification of auxotrophic mutants of the yeast Kluyveromyces marxianus by non‐homologous end joining‐mediated integrative transformation with genes from Saccharomyces cerevisiae

Rinji Akada, Mikiko Nakamura, Tohru Yarimizu, 2013, Yeast.

Designed construction of recombinant DNA at the ura3Δ0 locus in the yeast Saccharomyces cerevisiae

Mikiko Nakamura, H. Hoshida, R. Akada, 2013, Yeast.

Construction of a URA3 deletion strain from the allotetraploid bottom‐fermenting yeast Saccharomyces pastorianus

H. Hoshida, R. Akada, T. Ogata, 2012, Yeast.

Fusion PCR via novel overlap sequences.

H. Hoshida, R. Akada, Kamonchai Cha‐aim, 2012, Methods in molecular biology.

Reliable fusion PCR mediated by GC-rich overlap sequences.

H. Hoshida, R. Akada, Kamonchai Cha‐aim, 2009, Gene.

Combinatorial Gene Overexpression and Recessive Mutant Gene Introduction in Sake Yeast

H. Hoshida, R. Akada, T. Miyakawa, 2009, Bioscience, biotechnology, and biochemistry.

PCR‐mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae

Akihiko Kondo, Kenji Kida, Shigeru Morimura, 2006, Yeast.

Genome-Wide Analysis of Cellular Response to Bacterial Genotoxin CdtB in Yeast

H. Hoshida, R. Akada, Takao Kitagawa, 2007, Infection and Immunity.

Partial characterization of the mating-inducing factors (gamones) produced by mating type VI cells in the ciliate Euplotes patella syngen 2

R. Akada, 1986 .

Genes Required for Transcription of STA1 encoding an Extracellular Glucoamylase in the Yeast Saccharomyces

R. Akada, I. Yamashita, H. Yoshimoto, 1989 .

Marker-disruptive gene integration and URA3 recycling for multiple gene manipulation in Saccharomyces cerevisiae

Hideo Noda, Akihiko Kondo, Hideki Fukuda, 2009, Applied Microbiology and Biotechnology.

Elevated temperature greatly improves transformation of fresh and frozen competent cells in yeast.

R. Akada, Y. Nishizawa, M. Kawahata, 2000, BioTechniques.

Noncanonical recognition and UBL loading of distinct E2s by autophagy-essential Atg7

F. Inagaki, Y. Fujioka, N. Noda, 2012, Nature Structural &Molecular Biology.

Spontaneous selfing (intraclonal conjugation) in the ciliate Euplotes patella syngen 2 takes place without a change in mating type expression

R. Akada, 1986 .

Mating types and mating-inducing factors (gamones) in the ciliate Euplotes patella syngen 2

R. Akada, 1985 .

Enhancement of Hydrogen Production by Combining a Blend Ratio for Two Types of Algal Waste Biomass and Reducing Casamino Acids Supplementation By Clostridium butyricum RAK25832

R. Akada, Koichi Yamamoto, T. Imai, 2018 .

Enzymatic Hydrolysis of Black Liquor Xylan by a Novel Xylose-Tolerant, Thermostable β-Xylosidase from a Tropical Strain of Aureobasidium pullulans CBS 135684

R. Akada, P. Lotrakul, S. Prasongsuk, 2018, Applied Biochemistry and Biotechnology.

DNA extraction method for screening yeast clones by PCR.

R. Akada, Y. Nishizawa, T. Murakane, 2000, BioTechniques.

Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus

Mikiko Nakamura, Tohru Yarimizu, H. Hoshida, 2015, Microbial Cell Factories.

Identification of factors that accelerate hydrogen production by Clostridium butyricum RAK25832 using casamino acids as a nitrogen source

R. Akada, Koichi Yamamoto, T. Imai, 2017 .

High-Temperature Bioethanol Fermentation by Conventional and Nonconventional Yeasts

H. Hoshida, R. Akada, 2017 .

The Potential use of Trichoderma Viride Strain FRP3 in Biodegradation of the Herbicide Glyphosate

Tohru Yarimizu, R. Akada, T. Imai, 2013 .

Identification of genes that enhance cellulase protein production in yeast.

Haruo Takahashi, Rinji Akada, Kenro Tokuhiro, 2011, Journal of biotechnology.

Enhanced production of extracellular inulinase by the yeast Kluyveromyces marxianus in xylose catabolic state.

H. Hoshida, R. Akada, Kenta Kidera, 2018, Journal of bioscience and bioengineering.

Screening of Drugs That Suppress Ste11 MAPKKK Activation in Yeast Identified a c-Abl Tyrosine Kinase Inhibitor

Hidetoshi Takahashi, H. Hoshida, R. Akada, 2007, Bioscience, biotechnology, and biochemistry.

N-glycosylation deficiency enhanced heterologous production of a Bacillus licheniformis thermostable α-amylase in Saccharomyces cerevisiae

H. Hoshida, R. Akada, Tsuneyasu Fujita, 2013 .

A positive selection for plasmid loss in Saccharomyces cerevisiae using galactose‐inducible growth inhibitory sequences

R. Akada, Y. Nishizawa, M. Kawahata, 1999, Yeast.

The presence of an intron relieves gene repression caused by promoter-proximal four-bp specific sequences in yeast.

H. Hoshida, R. Akada, Hiroki Kikuta, 2023, Biochimica et biophysica acta. Gene regulatory mechanisms.

Characterization of an interesting novel mutant strain of commercial Saccharomyces cerevisiae

K. Ghaedi, H. Zarkesh-Esfahani, R. Akada, 2011 .

Quick-check method to test the size of Escherichia coli plasmids.

R. Akada, 1994, BioTechniques.

Yeast src homology region 3 domain-binding proteins involved in bud formation

R. Akada, Y. Matsui, R. Akada, 1996, The Journal of cell biology.

Development of a Novel Functional High-Throughput Screening System for Pathogen Effectors in the Yeast Saccharomyces cerevisiae

I. Yanatori, F. Kishi, R. Akada, 2009, Bioscience, biotechnology, and biochemistry.