S. Bearne

发表

Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions

Shan Ying, E. Pringle, Brett A. Duguay, 2022, bioRxiv.

The role of Brønsted base basicity in estimating carbon acidity at enzyme active sites: a caveat.

S. Bearne, 2019, Organic & biomolecular chemistry.

Glutamate gamma-semialdehyde as a natural transition state analogue inhibitor of Escherichia coli glucosamine-6-phosphate synthase.

R. Wolfenden, S. Bearne, 1995, Biochemistry.

Biophysical Analysis of Bacterial CTP Synthase Filaments Formed in the Presence of the Chemotherapeutic Metabolite Gemcitabine-5'-triphosphate.

S. Bearne, Gregory D. McCluskey, 2018, Journal of molecular biology.

Anfractuous assemblies of IMP dehydrogenase and CTP synthase: new twists on regulation?

S. Bearne, Gregory D. McCluskey, 2018, The FEBS journal.

GTP-Dependent Regulation of CTP Synthase: Evolving Insights into Allosteric Activation and NH3 Translocation

S. Bearne, Ji-Long Liu, Chenghao Guo, 2022, Biomolecules.

An assay for cytidine 5(')-triphosphate synthetase glutaminase activity using high performance liquid chromatography.

S. Bearne, Akshai Iyengar, 2002, Analytical biochemistry.

Slow-Onset, Potent Inhibition of Mandelate Racemase by 2-Formylphenylboronic Acid. An Unexpected Adduct Clasps the Catalytic Machinery.

S. Bearne, Colin D. Douglas, M. St. Maurice, 2021, Biochemistry.

Inhibition of CTP synthase from Escherichia coli by xanthines and uric acids.

S. Bearne, A. C. Roy, Faylene A Lunn, 2010, Bioorganic & medicinal chemistry letters.

Measuring benzohydroxamate complexation with Mg2+, Mn2+, Co2+, and Ni2+ using isothermal titration calorimetry

S. Bearne, Matthew Harty, 2016, Journal of Thermal Analysis and Calorimetry.

Potent inhibition of mandelate racemase by a fluorinated substrate-product analogue with a novel binding mode.

S. Bearne, M. St. Maurice, M. Nagar, 2014, Biochemistry.

Inactivation of Mandelate Racemase by 3-Hydroxypyruvate Reveals a Potential Mechanistic Link between Enzyme Superfamilies.

S. Bearne, M. St. Maurice, Brittney N. Wyatt, 2015, Biochemistry.

A platform for chemical modification of mandelate racemase: characterization of the C92S/C264S and &ggr;-thialysine 166 variants

S. Bearne, H. Kumar, M. Nagar, 2018, Protein engineering, design & selection : PEDS.

An assay for mandelate racemase using high-performance liquid chromatography.

S. Bearne, M. St. Maurice, M. D. Vaughan, 1999, Analytical biochemistry.

Synthesis of coenzyme A thioesters using methyl acyl phosphates in an aqueous medium.

S. Bearne, M. Pal, 2014, Organic & biomolecular chemistry.

Aspartate-107 and leucine-109 facilitate efficient coupling of glutamine hydrolysis to CTP synthesis by Escherichia coli CTP synthase.

S. Bearne, Akshai Iyengar, 2003, The Biochemical journal.

Purification of recombinant mandelate racemase: improved catalytic activity.

S. Bearne, Ariun Narmandakh, 2010, Protein expression and purification.

Perturbing the hydrophobic pocket of mandelate racemase to probe phenyl motion during catalysis.

C. Blouin, S. Bearne, Haiyan Jiang, 2005, Biochemistry.

Oxidative stress response in the opportunistic oral pathogen Fusobacterium nucleatum

S. Bearne, D. Barnett, J. Potrykus, 2011, Proteomics.

Direct selective pyrophosphorylation of the primary hydroxyl group in (hydroxyethyl)thiamin by modified phosphoric acid-cresol solutions and evaluation of extension of the method to nucleosides

G. Gish, K. Karimian, S. Bearne, 1989 .

Thiopurines inhibit coronavirus Spike protein processing and incorporation into progeny virions

Shan Ying, E. Pringle, Brett A. Duguay, 2022, bioRxiv.

Supporting Information for Proteomics DOI 10.1002/pmic.200700437

Robert L. White, S. Bearne, J. Potrykus, 2008 .

Illustrating Enzyme Inhibition Using Gibbs Energy Profiles

S. Bearne, 2012 .

Inhibition of Escherichia coliGlucosamine-6-phosphate Synthase by Reactive Intermediate Analogues

C. Blouin, S. Bearne, 2000, The Journal of Biological Chemistry.

Alternative substrates for wild-type and L109A E. coli CTP synthases: kinetic evidence for a constricted ammonia tunnel.

S. Bearne, Faylene A Lunn, Faylene A. Lunn, 2004, European journal of biochemistry.

Kinetic evidence for a constricted ammonia tunnel

S. Bearne, Faylene A Lunn, Faylene A. Lunn, 2004 .

Ground State, Intermediate, and Multivalent Nucleotide Analogue Inhibitors of Cytidine 5′‐Triphosphate Synthase

Scott D. Taylor, S. Bearne, Faylene A Lunn, 2008, ChemMedChem.

The interdigitating loop of the enolase superfamily as a specificity binding determinant or 'flying buttress'.

S. Bearne, 2017, Biochimica et biophysica acta. Proteins and proteomics.

Capturing the free energy of transition state stabilization: insights from the inhibition of mandelate racemase

S. Bearne, 2023, Philosophical Transactions of the Royal Society B.

An additional role for the Brønsted acid-base catalysts of mandelate racemase in transition state stabilization.

S. Bearne, M. Nagar, 2015, Biochemistry.

Inhibition of mandelate racemase by the substrate-intermediate-product analogue 1,1-diphenyl-1-hydroxymethylphosphonate.

S. Bearne, R. K. Burley, 2005, Bioorganic & medicinal chemistry letters.

Inhibition of mandelate racemase by α-fluorobenzylphosphonates

Scott D. Taylor, S. Bearne, M. Maurice, 2003 .

Phosphoenol acetylphosphonates: Substrate analogues as inhibitors of phosphoenolpyruvate enzymes

S. Bearne, R. Kluger, 1992 .

Inhibition of Escherichia coli glucosamine-6-phosphate synthase by reactive intermediate analogues. The role of the 2-amino function in catalysis.

C. Blouin, S. Bearne, 2000, The Journal of biological chemistry.

Unexpected Side Product Formed during LDA-induced Phosphonylation of Uridine

Palash Bhar, S. Bearne, 2017 .

An unsymmetrical approach to the synthesis of bismethylene triphosphate analogues.

Scott D. Taylor, S. Bearne, F. Mirzaei, 2006, Organic letters.

Reactivity and mechanism in chemical and synthetic biology

N. Richards, E. Parker, S. Bearne, 2023, Philosophical Transactions of the Royal Society B.

Potent Inhibition of Mandelate Racemase by Boronic Acids: Boron as a Mimic of a Carbon Acid Center.

S. Bearne, M. St. Maurice, L. Grandinetti, 2020, Biochemistry.

Electrochemical quartz crystal nanobalance (EQCN) studies of the adsorption behaviour of an enzyme, mandelate racemase, and its substrate, mandelic acid, on Pt

C. Brosseau, S. Bearne, S. G. Roscoe, 2005 .

Exploring the Potent Inhibition of CTP Synthase by Gemcitabine‐5′‐Triphosphate

Scott D. Taylor, S. Bearne, S. Mohamady, 2016, Chembiochem : a European journal of chemical biology.

Active site-directed inactivation of Escherichia coli glucosamine-6-phosphate synthase. Determination of the fructose 6-phosphate binding constant using a carbohydrate-based inactivator.

S. Bearne, 1996, The Journal of biological chemistry.

The role of lysine residues 297 and 306 in nucleoside triphosphate regulation of E. coli CTP synthase: inactivation by 2',3'-dialdehyde ATP and mutational analyses.

S. Bearne, Faylene A Lunn, Travis J Macleod, 2006, Biochimica et biophysica acta.

Proteomic investigation of glucose metabolism in the butyrate‐producing gut anaerobe Fusobacterium varium

Robert L. White, Brandi L. Mahaney, S. Bearne, 2007, Proteomics.

Proteomic investigation of amino acid catabolism in the indigenous gut anaerobe Fusobacterium varium

Robert L. White, S. Bearne, J. Potrykus, 2008, Proteomics.

Potent dialkyl substrate-product analogue inhibitors and inactivators of α-methylacyl-coenzyme A racemase from Mycobacterium tuberculosis by rational design.

S. Bearne, M. Pal, Nicole M Easton, 2018, Bioorganic chemistry.

INHIBITION OF β‐GALACTOSIDASE OF APPLE BY FLAVONOIDS AND OTHER POLYPHENOLS

S. Bearne, A. J. Dick, 1988 .

Intermediate analogue inhibitors of mandelate racemase: N-Hydroxyformanilide and cupferron.

S. Bearne, Jennifer R Bourque, R. K. Burley, 2007, Bioorganic & medicinal chemistry letters.

Rational design and synthesis of substrate-product analogue inhibitors of α-methylacyl-coenzyme A racemase from Mycobacterium tuberculosis.

S. Thirumalairajan, M. Khanal, S. Bearne, 2016, Chemical communications.

Reduction of intrinsic kinetic and thermodynamic barriers for enzyme-catalysed proton transfers from carbon acid substrates.

R. Spiteri, S. Bearne, 2005, Journal of theoretical biology.

Through the looking glass: Chiral recognition of substrates and products at the active sites of racemases and epimerases.

S. Bearne, 2020, Chemistry.

Inhibition of Escherichia coli CTP synthase by glutamate gamma-semialdehyde and the role of the allosteric effector GTP in glutamine hydrolysis.

S. Bearne, O. Hekmat, J. E. Macdonnell, 2001, The Biochemical journal.

Interrogation of the active site of OMP decarboxylase from Escherichia coli with a substrate analogue bearing an anionic group at C6.

S. Thirumalairajan, Brandi L. Mahaney, S. Bearne, 2010, Chemical communications.

Construction and Characterization of a Single-Chain Variable Fragment Antibody Library against Fusobacterium nucleatum

Yung-Hua Li, S. Halperin, S. Bearne, 2012 .

Inhibition of serine and proline racemases by substrate-product analogues.

S. Bearne, C. Legay, Matthew Harty, 2014, Bioorganic & medicinal chemistry letters.

A continuous assay for alpha-methylacyl-coenzyme A racemase using circular dichroism.

S. Bearne, D. Ouazia, 2010, Analytical biochemistry.

Altering the binding determinant on the interdigitating loop of mandelate racemase shifts specificity towards that of d-tartrate dehydratase.

S. Bearne, J. Hayden, Oliver P. Kuehm, 2022, Archives of biochemistry and biophysics.

Kinetic characterization and quaternary structure of glutamate racemase from the periodontal anaerobe Fusobacterium nucleatum.

S. Bearne, J. Potrykus, J. Flemming, 2009, Archives of biochemistry and biophysics.

Catalytic properties of the metal ion variants of mandelate racemase reveal alterations in the apparent electrophilicity of the metal cofactor.

S. Bearne, Matthew Harty, A. Sharma, 2019, Metallomics : integrated biometal science.

Heat Shock Proteins in the "Hot" Mitochondrion: Identity and Putative Roles.

Mohamed A. Nasr, N. El-Badri, S. Bearne, 2019, BioEssays : news and reviews in molecular, cellular and developmental biology.

Enzymic hydration of an olefin: the burden borne by fumarase

R. Wolfenden, S. Bearne, 1995 .

Activation and inhibition of CTP synthase from Trypanosoma brucei, the causative agent of African sleeping sickness.

S. Bearne, Craig H Steeves, 2011, Bioorganic & medicinal chemistry letters.

Gemcitabine is a Competitive Inhibitor of Cytidine‐5′‐triphosphate Synthase (CTPS) that Induces Enzyme Filament Polymerization

S. Bearne, Gregory D. McCluskey, 2015 .

A Paradigm for CH Bond Cleavage: Structural and Functional Aspects of Transition State Stabilization by Mandelate Racemase.

S. Bearne, M. St. Maurice, 2017, Advances in protein chemistry and structural biology.

Redefining the minimal substrate tolerance of mandelate racemase. Racemization of trifluorolactate.

S. Bearne, M. Nagar, Ariun Narmandakh, 2011, Biochemistry.

Hydrophobic nature of the active site of mandelate racemase.

S. Bearne, Martin St Maurice, Stephen L Bearne, 2004, Biochemistry.

A continuous assay for l-talarate/galactarate dehydratase using circular dichroism.

S. Bearne, Nicole M Easton, Sarah A E Aboushawareb, 2018, Analytical biochemistry.

Altering the Y137-K164-K166 triad of mandelate racemase and its effect on the observed pKa of the Brønsted base catalysts.

S. Bearne, Colin D. Douglas, M. Nagar, 2019, Archives of biochemistry and biophysics.

Mutational analysis of the active site flap (20s loop) of mandelate racemase.

S. Bearne, Jennifer R Bourque, 2008, Biochemistry.

Reactive architecture profiling with a methyl acyl phosphate electrophile.

S. Bearne, Laura C. McGary, Gemma L. Regan, 2023, Biochimica et biophysica acta. Proteins and proteomics.

Changes in quaternary structure cause a kinetic asymmetry of glutamate racemase‐catalyzed homocysteic acid racemization

S. Bearne, Joanna Mackie, H. Kumar, 2018, FEBS letters.

Inhibition of E. coli CTP synthase by the "positive" allosteric effector GTP.

S. Bearne, Faylene A Lunn, J. E. Macdonnell, 2004, Biochimica et biophysica acta.

Mutational analysis of conserved glycine residues 142, 143 and 146 reveals Gly(142) is critical for tetramerization of CTP synthase from Escherichia coli.

S. Bearne, Faylene A Lunn, Travis J Macleod, 2008, The Biochemical journal.

A metal‐dependent conformational change provides a structural basis for the inhibition of CTP synthase by gemcitabine‐5′‐triphosphate

S. Bearne, Norman Tran, T. Holyoak, 2023, Protein science : a publication of the Protein Society.

"Pinching" the ammonia tunnel of CTP synthase unveils coordinated catalytic and allosteric-dependent control of ammonia passage.

S. Bearne, Gregory D. McCluskey, 2018, Biochimica et biophysica acta. General subjects.

Structural Requirements for the Activation of Escherichia coli CTP Synthase by the Allosteric Effector GTP Are Stringent, but Requirements for Inhibition Are Lax*

S. Bearne, Faylene A Lunn, J. E. Macdonnell, 2008, Journal of Biological Chemistry.

Limited proteolysis of Escherichia coli cytidine 5'-triphosphate synthase. Identification of residues required for CTP formation and GTP-dependent activation of glutamine hydrolysis.

S. Bearne, Akshai Iyengar, D. Simard, 2003, European journal of biochemistry.

Construction and Characterization of a Single-Chain Variable Fragment Antibody Library against Fusobacterium nucleatum

Yung-Hua Li, S. Halperin, S. Bearne, 2012 .

Purification and characterization of β‐methylaspartase from Fusobacterium varium

Robert L. White, S. Bearne, S. Bahrami, 2001, Molecular and Cellular Biochemistry.

Structure of mandelate racemase with bound intermediate analogues benzohydroxamate and cupferron.

S. Bearne, M. St. Maurice, M. Nagar, 2012, Biochemistry.

Characterization of a novel, cytokine-inducible carboxypeptidase D isoform in haematopoietic tumour cells.

C. K. Too, S. Bearne, S. Abdelmagid, 2005, The Biochemical journal.

A Phenylboronic Acid-Based Transition State Analogue Yields Nanomolar Inhibition of Mandelate Racemase.

S. Bearne, J. Hayden, Oliver P. Kuehm, 2023, Biochemistry.

Asymmetry in catalysis: ‘unidirectional’ amino acid racemases

S. Bearne, 2021, The Biochemist.

Mandelate racemase in pieces: effective concentrations of enzyme functional groups in the transition state.

R. Wolfenden, S. Bearne, 1997, Biochemistry.

Rational design and synthesis of substrate-product analogue inhibitors of α-methylacyl-coenzyme A racemase from Mycobacterium tuberculosis.

S. Thirumalairajan, M. Khanal, S. Bearne, 2016, Chemical communications.

Substrate-product analogue inhibitors of isoleucine 2-epimerase from Lactobacillus buchneri by rational design.

S. Bearne, Gregory D. McCluskey, Joshua W. M. MacMillan, 2019, Organic & biomolecular chemistry.

Interrogating l-fuconate dehydratase with tartronate and 3-hydroxypyruvate reveals subtle differences within the mandelate racemase-subgroup of the enolase superfamily.

S. Bearne, Colin D. Douglas, Oliver P. Kuehm, 2024, Archives of biochemistry and biophysics.