M. Tam

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Site-directed mutagenesis and chemical modification of histidine residues on an alpha-class chick liver glutathione S-transferase CL 3-3. Histidines are not needed for the activity of the enzyme and diethylpyrocarbonate modifies both histidine and lysine residues.

L. Chang, M. Tam, 1993, European journal of biochemistry.

Expression and characterization of SUMO-conjugated metal-responsive transcription factor 1: SIM-dependent cross-interaction and distinct DNA binding activity.

Lih-Yuan Lin, M. Tam, Chang-Yi Lin, 2013, Journal of biochemistry.

The Role of Small Ubiquitin-like Modifier-interacting Motif in the Assembly and Regulation of Metal-responsive Transcription Factor 1*

Lih-Yuan Lin, M. Tam, Ya-Chuan Liu, 2011, The Journal of Biological Chemistry.

Production of unmodified human adult hemoglobin in Escherichia coli.

V. Simplaceanu, C. Ho, N. T. Ho, 1993, Proceedings of the National Academy of Sciences of the United States of America.

Identification of the heterogeneous nuclear ribonucleoprotein A2/B1 as the antigen for the gastrointestinal cancer specific monoclonal antibody MG7

D. Fan, D. Fan, M. Tam, 2005, Proteomics.

Advances in Biomedical Research

J. Coligan, Sung-Liang Yu, R. Daniel, 1998, Journal of Biomedical Science.

PTEN interacts with metal-responsive transcription factor 1 and stimulates its transcriptional activity.

Lih-Yuan Lin, M. Tam, Ya-Chuan Liu, 2012, The Biochemical journal.

Recombinant Octameric Hemoglobins as Resuscitation Fluids in a Murine Model of Traumatic Brain Injury Plus Hemorrhagic Shock

P. Kochanek, C. Ho, V. Simplaceanu, 2013 .

Phenotypic analysis of proteinase A mutants. Implications for autoactivation and the maturation pathway of the vacuolar hydrolases of Saccharomyces cerevisiae.

M. Innis, M. Tam, C. Woolford, 1993, The Journal of biological chemistry.

Electroblotting onto glass-fiber filter from an analytical isoelectrofocusing gel: a preparative method for isolating proteins for N-terminal microsequencing.

J. Hsieh, M. Tam, F. Lin, 1988, Analytical biochemistry.

New crystal forms of a mu-class glutathione S-transferase from rat liver.

J. Rose, B. Wang, M. Tam, 1993 .

The single cysteine residue on an alpha family chick liver glutathione S-transferase CL 3-3 is not functionally important.

L. Chang, M. Tam, L. Y. Wang, 1991, Biochemical and biophysical research communications.

Reversible modification of rat liver glutathione S-transferase 3-3 with 1-chloro-2,4-dinitrobenzene: specific labelling of Tyr-115.

L. Liu, J. Hsieh, M. Tam, 1993, The Biochemical journal.

Site-directed mutagenesis and chemical modification of cysteine residues of rat glutathione S-transferase 3-3.

J. Hsieh, M. Tam, S. Tsai, 1992, The Biochemical journal.

Modification of glutathione S-transferase 3-3 mutants with 2-(S-glutathionyl)-3,5,6-trichloro-1,4-benzoquinone. Identification of the C-terminal tryptic fragment as part of the H-site and evidence that 2-(S-glutathionyl)-3,5,6-trichloro-1,4-benzoquinone is not specific for cysteine labelling.

C. Hsiao, L. Liu, M. Tam, 1994, Biochemical Journal.

Amino acid sequencing, molecular cloning and modelling of the chick liver class-theta glutathione S-transferase CL1.

C. Hsiao, E. Martsen, M. Tam, 1995, The Biochemical journal.

Crystals of isoenzyme 3-3 of rat liver glutathione S-transferase with and without inhibitor.

J. Rose, B. Wang, M. Tam, 1991, Acta crystallographica. Section B, Structural science.

Cysteine-86 is not needed for the enzymic activity of glutathione S-transferase 3-3.

J. Hsieh, Y. Lai, M. Tam, 1991, The Biochemical journal.

Characterization and heterospecific expression of cDNA clones of genes in the maize GSH S-transferase multigene family.

K. P. Timmerman, M. Tam, N. Li, 1988, Nucleic acids research.

Nucleotide sequence of a class mu glutathione S-transferase from chicken liver.

L. Liu, M. Tam, 1991, Biochimica et biophysica acta.

Cloning and expression of a chick liver glutathione S-transferase CL 3 subunit with the use of a baculovirus expression system.

L. Liu, L. Chang, M. Tam, 1992, Biochemical Journal.

Mass spectrometric analysis of rat liver cytosolic glutathione S-transferases: modifications are limited to N-terminal processing.

M. Tam, S. Tsai, L. Y. Wang, 1995, The Biochemical journal.

Human glutathione S-transferases. The Ha multigene family encodes products of different but overlapping substrate specificities.

M. Tam, C. Kao-Shan, C. Tu, 1988, The Journal of biological chemistry.

Characterization of glutathione S-transferases from day-old chick livers.

L. Chang, M. Tam, C. Tu, 1990, Biochemistry.

Identification of rat liver glutathione S‐transferase Yb subunits by partial N‐terminal sequencing after electroblotting of proteins onto a polyvinylidene difluoride membrane from an analytical isoelectric focusing gel

J. Hsieh, L. Chang, M. Tam, 1990, Electrophoresis.

Nucleotide sequence of class-α glutathione S-transferases from chicken liver

M. Tam, Li-Fan Liu, S. Wu, 1993 .

Production of human normal adult and fetal hemoglobins in Escherichia coli.

D. P. Sun, V. Simplaceanu, C. Ho, 1997, Protein engineering.

cDNA cloning and immunologic characterization of Pen o 18, the vacuolar serine protease major allergen of Penicillium oxalicum.

C. Wang, S. Han, S. R. Wang, 2001, The Journal of laboratory and clinical medicine.

Pen ch 13 allergen induces secretion of mediators and degradation of occludin protein of human lung epithelial cells

M. Tam, D. Perng, H. Tai, 2006, Allergy.

Detection of dimethylarginines in protein hydrolysates by matrix-assisted laser desorption/ionization mass spectrometry.

M. Tam, C. Hsieh, 2006, Analytical biochemistry.

Matrix-assisted laser desorption/ionization of peptides on AnchorChip targets with alpha-cyano-4-hydroxycinnamic acid and nitrocellulose as matrix.

M. Tam, Yu-Ching Wu, C. Hsieh, 2006, Rapid communications in mass spectrometry : RCM.

Vacuolar Serine Protease Is a Major Allergen of Cladosporium cladosporioides

M. Tam, R. Panzani, H. Tai, 2008, International Archives of Allergy and Immunology.

A Vacuolar Serine Protease (Rho m 2) Is a Major Allergen of Rhodotorula mucilaginosa and Belongs to a Class of Highly Conserved Pan-Fungal Allergens

M. Tam, H. Tai, H. Chou, 2005, International Archives of Allergy and Immunology.

cDNA Cloning, Biological and Immunological Characterization of the Alkaline Serine Protease Major Allergen from Penicillium chrysogenum

M. Tam, M. Chou, S. Han, 2002, International Archives of Allergy and Immunology.

Sickle Cell Hemoglobin with Mutation at αHis-50 Has Improved Solubility*

C. Ho, V. Simplaceanu, N. T. Ho, 2015, The Journal of Biological Chemistry.

Molecular and structural analysis of immunoglobulin E‐binding epitopes of Pen ch 13, an alkaline serine protease major allergen from Penicillium chrysogenum

M. Tam, H. Tai, H. Chou, 2004, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

Autoxidation and Oxygen Binding Properties of Recombinant Hemoglobins with Substitutions at the αVal-62 or βVal-67 Position of the Distal Heme Pocket*

C. Ho, V. Simplaceanu, N. T. Ho, 2013, The Journal of Biological Chemistry.

The 30 kDa protein co-purified with chick liver glutathione S-transferases is a carbonyl reductase.

M. Tam, S. Tsai, L. Y. Wang, 1996, Biochimica et biophysica acta.

Expression of Yb1 glutathione S-transferase using a baculovirus expression system.

L. Liu, J. Hsieh, M. Tam, 1989, Biochemical and Biophysical Research Communications - BBRC.

Murine malignant cells synthesize a 19,000-dalton protein that is physicochemically and antigenically related to the immunosuppressive retroviral protein, P15E

R. Snyderman, M. Tam, G. Cianciolo, 1983, The Journal of experimental medicine.

Identification of galectin I and thioredoxin peroxidase II as two arsenic-binding proteins in Chinese hamster ovary cells.

M. Tam, Te-Chang Lee, Rong-Nan Huang, 2003, The Biochemical journal.

New look at hemoglobin allostery.

C. Ho, V. Simplaceanu, M. Tam, 2015, Chemical reviews.

Tyr115, gln165 and trp209 contribute to the 1, 2-epoxy-3-(p-nitrophenoxy)propane-conjugating activity of glutathione S-transferase cGSTM1-1.

C. Hsiao, L. Liu, C. Chou, 2000, Journal of molecular biology.

The three-dimensional structure of an avian class-mu glutathione S-transferase, cGSTM1-1 at 1.94 A resolution.

Y. J. Sun, C. Hsiao, M. Tam, 1998, Journal of molecular biology.

Complementary DNA cloning and immunologic characterization of a new Penicillium citrinum allergen (Pen c 3).

C. Wang, S. R. Wang, M. Tam, 2000, The Journal of allergy and clinical immunology.

Asp159 is a critical core amino acid of an IgE-binding and cross-reactive epitope of a dust mite allergen Der f 7.

M. Tam, H. Tai, R. Tang, 2011, Molecular immunology.

Homology modeling and monoclonal antibody binding of the Der f 7 dust mite allergen

W. Thomas, M. Tam, S. Sheu, 2011, Immunology and cell biology.

Characterization of the 33‐kilodalton major allergen of Penicillium citrinum by using MoAbs and N‐terminal amino acid sequencing

M. Tam, H. Shen, W.‐L. Lin, 1997, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

Transaldolases are novel and immunoglobulin E cross‐reacting fungal allergens

M. Tam, H. Tai, H. Chou, 2011, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

Characterization of Enolase Allergen from Rhodotorula mucilaginosa

M. Tam, R. Tang, H. Chou, 2002, Journal of Biomedical Science.

The Importance of Serine Proteinases as Aeroallergens Associated with Asthma

M. Tam, S. Han, H. Chou, 1999, International Archives of Allergy and Immunology.

Alkaline Serine Proteinase: A Major Allergen of Aspergillus oryzae and Its Cross-Reactivity with Penicillium citrinum

J. Tsai, M. Tam, S. Han, 1998, International Archives of Allergy and Immunology.

Amino Acid Residues Involved in the Functional Integrity of Escherichia coli Methionine Aminopeptidase

Lih-Yuan Lin, M. Tam, Chao-Zong Lee, 1999, Journal of bacteriology.

Developmental changes of heat-shock proteins in porcine testis by a proteomic analysis.

San-Yuan Huang, M. Tam, Ming-yu Chen, 2005, Theriogenology.

Roles of α114 and β87 Amino Acid Residues in the Polymerization of Hemoglobin S: Implications for Gene Therapy

D. P. Sun, C. Ho, R. Nagel, 1996 .

Primary sequence of duck metallothionein.

L. Liu, C. Fenselau, P. C. Huang, 1990, Biochimica et biophysica acta.

Isolation and molecular cloning of human sorcin a calcium-binding protein in vincristine-resistant H0B1 lymphoma cells

M. Kao, M. Tam, S. Pai, 1995 .

Yeast Ribosomal Protein L12 Is a Substrate of Protein-arginine Methyltransferase 2*

M. Tam, M. Chern, T. C. Tam, 2002, The Journal of Biological Chemistry.

Physical characteristics of the reconstitution intermediates (RI30 and RI30*) from the 30S ribosomal subunit of Escherichia coli.

M. Tam, W. Hill, 1981, Biochemistry.

Expression of glutathione peroxidase I gene in selenium-deficient rats.

M. Tam, C. C. Reddy, N. Li, 1988, Nucleic acids research.

Rpl12p affects the transcription of the PHO pathway high‐affinity inorganic phosphate transporters and repressible phosphatases

Wen-Yo Tu, M. Tam, Yu-chen Huang, 2011, Yeast.

Glutathione S-transferase pi in an arsenic-resistant Chinese hamster ovary cell line.

T. Lee, M. Tam, H. F. Wang, 1992, The Biochemical journal.

Physical characteristics of protein-deficient derived from the 50S ribosomal subunit.

M. Tam, W. Hill, L. Giri, 1976, Biochemistry.

Characterization of a phage specific to hemorrhagic Escherichia coli O157:H7 and disclosure of variations in host outer membrane protein ompC.

K. Wu, M. Tam, H. Ding, 1998, Journal of biomedical science.

Characterization of a Phage Specific to Hemorrhagic Escherichia coli O157:H7 and Disclosure of Variations in Host Outer Membrane Protein OmpC

Sung-Liang Yu, M. Tam, H. Ding, 1998, Journal of Biomedical Science.

Characterization of chicken-liver glutathione S-transferase (GST) A1-1 and A2-2 isoenzymes and their site-directed mutants heterologously expressed in Escherichia coli: identification of Lys-15 and Ser-208 on cGSTA1-1 as residues interacting with ethacrynic acid.

L. Liu, M. Tam, Y. Liaw, 1997, The Biochemical journal.

Escherichia coli methionine aminopeptidase with Tyr168 to alanine substitution can improve the N-terminal processing of recombinant proteins with valine at the penultimate position.

M. Tam, S. Tsai, C. Hsieh, 2004, Analytical biochemistry.

Purification and characterization of tilapia (Oreochromis mossambicus) deoxyribonuclease I--primary structure and cDNA sequence.

W. Y. Wang, M. Tam, Y. Hsiao, 1997, European journal of biochemistry.

Modified method for double stranded DNA sequencing and synthetic oligonucleotide purification.

J. Hsieh, C. Hui, M. Tam, 1988, Nucleic acids research.

ShyA, a membrane protein for proper septation of hyphae in Streptomyces.

M. Tam, S. Teng, M. Chern, 2006, Biochemical and biophysical research communications.

Aspergillus and Penicillium allergens: Focus on proteases

M. Tam, R. Tang, H. Chou, 2007, Current allergy and asthma reports.

Alkaline Serine Proteinase Is a Major Allergen of Aspergillus flavus, a Prevalent Airborne Aspergillus Species in the Taipei Area

M. Tam, S. Han, H. Chou, 1999, International Archives of Allergy and Immunology.

Characterization of a novel Candida albicans 29 kDa IgE‐binding protein – purification, cDNA isolation and heterologous expression of Cand a 3

M. Tam, H. Chou, H. Shen, 2003, Allergy.

The basement membrane glycoprotein entactin promotes cell attachment and binds calcium ions.

M. Tam, S. Chakravarti, A. Chung, 1990, The Journal of biological chemistry.

Characterization of the allergen Der f 7 from house dust mite extracts by species‐specific and crossreactive monoclonal antibodies

M. Tam, L. Tsai, Horng‐Der Shen, 1997, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

The Transaldolase, a Novel Allergen of Fusarium proliferatum, Demonstrates IgE Cross-Reactivity with Its Human Analogue

M. Tam, C. Yeh, H. Tai, 2014, PloS one.

Use of monoclonal antibodies to isolate and characterize Cyn d I, the major allergen of Bermuda grass pollen.

C. Liu, C. Chi, J. Tsai, 1993, The Journal of allergy and clinical immunology.

Expression of proteins with dimethylarginines in Escherichia coli for protein–protein interaction studies

San-Yuan Huang, M. Tam, Yu-Ching Wu, 2007, Protein science : a publication of the Protein Society.

Cloning of porcine neuron growth inhibitory factor (metallothionein III) cDNA and expression of the gene in Saccharomyces cerevisiae.

M. Tam, S. H. Wang, Chung-yu Chang, 1997, Gene.

High-level production of human a- and -globins in insect cells

M. Busch, S. Liebhaber, D. Groebe, 1992 .

Molecular and immunological characterization of Pen ch 18, the vacuolar serine protease major allergen of Penicillium chrysogenum

M. Tam, H. Chou, Horng‐Der Shen, 2003, Allergy.

Pen ch 13 Major Fungal Allergen Decreases CD44 Expression in Human Bronchial Epithelial Cells

M. Tam, H. Tai, H. Chou, 2010, International Archives of Allergy and Immunology.

Co-expression of glutathione S-transferase with methionine aminopeptidase: a system of producing enriched N-terminal processed proteins in Escherichia coli

Lih-Yuan Lin, M. Tam, T. C. Tam, 1999 .

Physical characteristics of 23 S rRNA from the 50 S ribosomal subunit of escherichia coli

M. Tam, J. Dodd, W. Hill, 1981, FEBS letters.

Physical characteristics of the reconstitution intermediate RI50(1) from the 50 S ribosomal subunit of Escherichia coli

M. Tam, W. Hill, 1980, FEBS letters.

Characterization of the isoforms of the group I allergen of Cynodon dactylon.

C. Liu, J. Tsai, M. Tam, 1995, The Journal of allergy and clinical immunology.

Physical characteristics of 16 S rRNA under reconstitution conditions.

M. Tam, J. Dodd, W. Hill, 1981, Journal of Biological Chemistry.

Proteomic analysis of stable protein methylation in lymphoblastoid cells.

M. Hsieh, Chuan Li, M. Tam, 2002, Journal of biochemistry.

Production and characterization of monoclonal antibodies to serine proteinase allergens in Penicillium and Aspergillus species

M. Tam, H. Chou, Horng‐Der Shen, 2000, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

The different modes of binding of the dust mite allergens, Der f 7 and Der p 7, on a monoclonal antibody WH9 contribute to the differential reactivity.

M. Tam, S. Sheu, C. Yeh, 2017, Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi.

Developmental expression of three mungbean Hsc70s and substrate-binding specificity of the encoded proteins.

Miin-Feng Wu, M. Tam, Yueh-hsiang Yu, 2004, Plant & cell physiology.

Localization of a carboxylic residue possibly involved in the inhibition of vacuolar H+-pyrophosphatase by N, N'-dicyclohexylcarbodi-imide.

S. Hung, M. Tam, R. Pan, 1999, The Biochemical journal.

Mass spectrometric analysis of rat ovary and testis cytosolic glutathione S-transferases (GSTs): identification of a novel class-alpha GST, rGSTA6*, in rat testis.

M. Tam, S. Tsai, C. Hsieh, 1997, The Biochemical journal.

cDNA Cloning and Immunological Characterization of a Newly Identified Enolase Allergen from Penicillium citrinum and Aspergillus fumigatus

J. Tsai, M. Tam, R. Tang, 2002, International Archives of Allergy and Immunology.

Isolation and Partial Characterization of a 46-kD Allergen of Bermuda Grass Pollen

C. Chi, M. Tam, Z. Chang, 2001, Journal of Biomedical Sciences.

Isolation and partial characterization of a 46-kd allergen of Bermuda grass pollen.

C. Chi, M. Tam, Z. Chang, 2001, Journal of Biomedical Sciences.

Co-expression of glutathione S-transferase with methionine aminopeptidase: a system of producing enriched N-terminal processed proteins in Escherichia coli.

M. Tam, T. C. Tam, I. Kuan, 1999, The Biochemical journal.

Restoring allosterism with compensatory mutations in hemoglobin.

D. P. Sun, C. Ho, N. T. Ho, 1994, Proceedings of the National Academy of Sciences of the United States of America.

Identification of vacuolar serine proteinase as a major allergen of Aspergillus fumigatus by immunoblotting and N‐terminal amino acid sequence analysis

M. Tam, S. Han, H. Chou, 2001, Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology.

Pen ch 13 Major Fungal Allergen Decreases CD44 Expression in Human Bronchial Epithelial Cells

M. Tam, H. Tai, H. Chou, 2010, International Archives of Allergy and Immunology.