J. Queiroz

发表

Bioreducible poly(2-ethyl-2-oxazoline)-PLA-PEI-SS triblock copolymer micelles for co-delivery of DNA minicircles and Doxorubicin.

Elisabete C. Costa, I. Correia, C. Pichon, 2015, Journal of controlled release : official journal of the Controlled Release Society.

Performance of a non-grafted monolithic support for purification of supercoiled plasmid DNA.

Â. Sousa, J. Queiroz, F. Sousa, 2011, Journal of chromatography. A.

Enhancement of a biotechnological platform for the purification and delivery of a human papillomavirus supercoiled plasmid DNA vaccine.

D. Costa, Â. Sousa, J. Queiroz, 2020, New biotechnology.

Purification of Membrane-Bound Catechol-O-Methyltransferase by Arginine-Affinity Chromatography

J. Queiroz, A. Q. Pedro, P. Pereira, 2015, Chromatographia.

First liquid chromatographic method for the simultaneous determination of amiodarone and desethylamiodarone in human plasma using microextraction by packed sorbent (MEPS) as sample preparation procedure.

J. Queiroz, A. Falcão, G. Alves, 2013, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Phenanthroline polyazamacrocycles as G-quadruplex DNA binders.

G. Salgado, J. Mergny, T. Quintela, 2018, Organic & biomolecular chemistry.

Characterization of a Parkinson's disease rat model using an upgraded paraquat exposure paradigm.

M. Beal, A. Salgado, N. Sousa, 2019, The European journal of neuroscience.

Plasmid DNA hydrogels for biomedical applications.

A. Valente, M. Miguel, D. Costa, 2014, Advances in colloid and interface science.

Responsive Plasmid DNA Hydrogels: A New Approach for Biomedical Applications

A. Valente, D. Costa, J. Queiroz, 2015 .

Plasmid DNA microgels for a therapeutical strategy combining the delivery of genes and anticancer drugs.

A. Valente, M. Miguel, D. Costa, 2012, Macromolecular bioscience.

Screening nucleotide binding to amino acid-coated supports by surface plasmon resonance and nuclear magnetic resonance

E. Cabrita, J. Queiroz, C. Cruz, 2011, Analytical and bioanalytical chemistry.

Pichia pastoris: a recombinant microfactory for antibodies and human membrane proteins.

J. Queiroz, F. Sousa, A. Q. Pedro, 2013, Journal of microbiology and biotechnology.

Preparative isolation of polymerase chain reaction products using mixed-mode chromatography.

J. Queiroz, L. Bülow, T. Matos, 2015, Analytical biochemistry.

Folate-Targeted Multifunctional Amino Acid-Chitosan Nanoparticles for Improved Cancer Therapy

Elisabete C. Costa, I. Correia, C. Pichon, 2014, Pharmaceutical Research.

New approach in RNA quantification using arginine-affinity chromatography: potential application in eukaryotic and chemically synthesized RNA

J. Queiroz, F. Sousa, R. Martins, 2013, Analytical and Bioanalytical Chemistry.

Application of a fed-batch bioprocess for the heterologous production of hSCOMT in Escherichia coli.

J. Queiroz, M. Bonifácio, L. Passarinha, 2009, Journal of microbiology and biotechnology.

Biofunctionalized nanoparticles with pH-responsive and cell penetrating blocks for gene delivery

I. Correia, V. Gaspar, J. Queiroz, 2013, Nanotechnology.

Nanoparticle mediated delivery of pure P53 supercoiled plasmid DNA for gene therapy.

I. Correia, Â. Sousa, V. Gaspar, 2011, Journal of Controlled Release.

Low-cost purification of nisin from milk whey to a highly active product

J. Teixeira, P. Mazzola, T. Penna, 2015 .

Preliminary studies on the use of cyanines as ligands in dye-affinity chromatography of proteins

J. Queiroz, A. Pardal, P. Almeida, 2002 .

The role of liquid-phase microextraction techniques in bioanalysis.

E. Gallardo, J. Queiroz, M. Barroso, 2015, Bioanalysis.

Separation of different forms of proteose peptone 3 by hydrophobic interaction chromatography with a dual salt system.

L. Rodrigues, J. Teixeira, J. Queiroz, 2008, Biomedical chromatography : BMC.

Capto™ Resins for Chromatography of DNA: A Minor Difference in Ligand Composition Greatly Influences the Separation of Guanidyl-Containing Fragments

J. Queiroz, L. Bülow, T. Matos, 2016, Chromatographia.

Binding and elution behavior of small deoxyribonucleic acid fragments on a strong anion-exchanger multimodal chromatography resin.

J. Queiroz, J. Queiroz, L. Bülow, 2013, Journal of chromatography. A.

An artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures

João M. L. Dias, J. Queiroz, A. Q. Pedro, 2015, Microbial Cell Factories.

Bioanalytical methods for the determination of cocaine and metabolites in human biological samples.

E. Gallardo, J. Queiroz, M. Barroso, 2009, Bioanalysis.

Hair: a complementary source of bioanalytical information in forensic toxicology.

E. Gallardo, J. Queiroz, M. López-Rivadulla, 2011, Bioanalysis.

Supercoiled plasmid quality assessment by analytical arginine-affinity chromatography.

J. Queiroz, F. Sousa, 2011, Journal of chromatography. A.

Biomedical application of plasmid DNA in gene therapy: A new challenge for chromatography

J. Queiroz, F. Sousa, L. Passarinha, 2009, Biotechnology & genetic engineering reviews.

Naphthalene amine support for G-quadruplex isolation.

J. Queiroz, F. Sousa, M. Corvo, 2017, The Analyst.

Plasmid purification by using a new naphthalene tripodal support

J. Queiroz, Tiago Santos, C. Tomaz, 2017 .

Performance of hydrophobic interaction ligands for human membrane-bound catechol-O-methyltransferase purification.

J. Queiroz, A. Q. Pedro, M. Bonifácio, 2013, Journal of separation science.

Comparative study on the interaction of recombinant human soluble catechol-O-methyltransferase on some hydrophobic adsorbents.

J. Queiroz, M. Bonifácio, L. Passarinha, 2007, Biomedical chromatography : BMC.

Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography.

D. Prazeres, J. Queiroz, F. Sousa, 2007, Biomedical chromatography : BMC.

The effects of ligand chain length, salt concentration and temperature on the adsorption of bovine serum albumin onto polypropyleneglycol-Sepharose.

J. Phillips, N. Pinto, J. Queiroz, 2005, Biomedical chromatography : BMC.

Effect of salts and temperature on the adsorption of bovine serum albumin on polypropylene glycol-Sepharose under linear and overloaded chromatographic conditions.

N. Pinto, J. Queiroz, A. C. Dias-Cabral, 2003, Journal of chromatography. A.

Assessment of purity and quantification of plasmid DNA in process solutions using high-performance hydrophobic interaction chromatography.

M. M. Diogo, D. Prazeres, J. Queiroz, 2003, Journal of chromatography. A.

Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized sepharose CL-6B. Using and relating two different models for describing the effect of salt and temperature on retention.

N. Pinto, M. M. Diogo, D. Prazeres, 2003, Journal of chromatography. A.

Comparative study on the fractionation of cellulases on some hydrophobic interaction chromatography adsorbents.

J. Queiroz, C. Tomaz, D. Duarte, 2002, Journal of chromatography. A.

Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B. Application of the solvophobic theory.

M. M. Diogo, D. Prazeres, J. Queiroz, 2002, Journal of chromatography. A.

Hydrophobic interaction chromatography of Chromobacterium viscosum lipase on polypropylene glycol immobilised on Sepharose.

M. M. Diogo, J. Queiroz, S. Silva, 1999, Journal of chromatography. A.

Separation and analysis of plasmid denatured forms using hydrophobic interaction chromatography.

M. M. Diogo, D. Prazeres, J. Queiroz, 1999, Analytical biochemistry.

Preliminary study on hydrophobic interaction chromatography of Chromobacterium viscosum lipase on polypropylene glycol immobilized on Sepharose.

M. M. Diogo, J. Queiroz, J. Cabral, 1998, Journal of chromatography. A.

Hydrophobic interaction chromatography of Chromobacterium viscosum lipase

J. Queiroz, J. Cabral, Fernando Garcia, 1995 .

Smoothing membrane protein structure determination by initial upstream stage improvements

J. Queiroz, A. Q. Pedro, L. Passarinha, 2019, Applied Microbiology and Biotechnology.

Development of plasmid DNA nanoparticles for mitochondrial gene therapy

D. Costa, J. Queiroz, F. Sousa, 2019, Materials for Biomedical Engineering.

Aptamer-guided acridine derivatives for cervical cancer.

J. Queiroz, Josué Carvalho, Jéssica Lopes-Nunes, 2019, Organic & biomolecular chemistry.

G-quadruplex, Friend or Foe: The Role of the G-quartet in Anticancer Strategies.

G. Salgado, J. Mergny, J. Queiroz, 2020, Trends in molecular medicine.

Amino acids–nucleotides biomolecular recognition: from biological occurrence to affinity chromatography

J. Queiroz, F. Sousa, C. Cruz, 2010, Journal of molecular recognition : JMR.

Affinity approaches in RNAi-based therapeutics purification.

J. Queiroz, F. Sousa, A. Figueiras, 2016, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

HPV‐16 targeted DNA vaccine expression: The role of purification

J. Tomás, Â. Sousa, J. Queiroz, 2018, Biotechnology progress.

Study of the interaction between indole-based compounds and biologically relevant G-quadruplexes.

G. Salgado, J. Mergny, J. Queiroz, 2017, Biochimie.

Recovery of biological active catechol-O-methyltransferase isoforms from Q-sepharose.

J. Queiroz, A. Q. Pedro, M. Bonifácio, 2014, Journal of separation science.

Histidine affinity chromatography-based methodology for the simultaneous isolation of Escherichia coli small and ribosomal RNA.

J. Queiroz, F. Sousa, R. Martins, 2012, Biomedical chromatography : BMC.

Anti-Helicobacter pylori and urease inhibitory activities of resveratrol and red wine

F. Domingues, E. Gallardo, J. Queiroz, 2011 .

Development and validation of an analytical method for the determination of trans- and cis-resveratrol in wine: analysis of its contents in 186 Portuguese red wines.

F. Domingues, E. Gallardo, J. Queiroz, 2011, Journal of agricultural and food chemistry.

Targeting of Mitochondria-Endoplasmic Reticulum by Fluorescent Macrocyclic Compounds

J. Queiroz, C. Cruz, S. Silvestre, 2011, PloS one.

Cancer gene therapy mediated by RALA/plasmid DNA vectors: Nitrogen to phosphate groups ratio (N/P) as a tool for tunable transfection efficiency and apoptosis.

T. Albuquerque, A. R. Neves, D. Costa, 2019, Colloids and surfaces. B, Biointerfaces.

Assessment of COMT isolation by HIC using a dual salt system and low temperature.

J. Queiroz, M. Bonifácio, L. Passarinha, 2009, Biomedical chromatography : BMC.

Dilemma on plasmid DNA purification: binding capacity vs selectivity.

J. Queiroz, F. Sousa, J. Valente, 2020, Journal of chromatography. A.

Gas-generating TPGS-PLGA microspheres loaded with nanoparticles (NIMPS) for co-delivery of minicircle DNA and anti-tumoral drugs.

Elisabete C. Costa, I. Correia, C. Pichon, 2015, Colloids and surfaces. B, Biointerfaces.

Evaluation of MutS and Mut+Pichia pastoris Strains for Membrane-Bound Catechol-O-Methyltransferase Biosynthesis

J. Queiroz, A. Q. Pedro, M. Bonifácio, 2015, Applied Biochemistry and Biotechnology.

A new approach on the purification of recombinant human soluble catechol-O-methyltransferase from an Escherichia coli extract using hydrophobic interaction chromatography.

J. Queiroz, P. Soares-da-Silva, M. Bonifácio, 2008, Journal of chromatography. A.

Plasmid DNA microgels for drug/gene co-delivery: A promising approach for cancer therapy

A. Valente, M. Miguel, D. Costa, 2014 .

Binding mechanisms for histamine and agmatine ligands in plasmid deoxyribonucleic acid purifications.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Journal of chromatography. A.

Discovery of Small Molecules as Membrane-Bound Catechol-O-methyltransferase Inhibitors with Interest in Parkinson’s Disease: Pharmacophore Modeling, Molecular Docking and In Vitro Experimental Validation Studies

E. Gallardo, J. Queiroz, S. Silvestre, 2021, Pharmaceuticals.

Aptamer-based Targeted Delivery of a G-quadruplex Ligand in Cervical Cancer Cells

G. Salgado, J. Mergny, J. Queiroz, 2019, Scientific Reports.

Methotrexate-plasmid DNA polyplexes for cancer therapy: Characterization, cancer cell targeting ability and tuned in vitro transfection

A. R. Neves, D. Costa, Â. Sousa, 2019, Journal of Molecular Liquids.

Purification of Plasmid DNA Vectors Produced in Escherichia coli for Gene Therapy and DNA Vaccination Applications

M. M. Diogo, D. Prazeres, J. Queiroz, 2005 .

Amino acid derivatized monoliths for purification of a DNA vaccine against influenza

J. Queiroz, F. Sousa, C. Tomaz, 2015, 2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG).

Selective purification of supercoiled plasmid DNA from clarified cell lysates with a single histidine–agarose chromatography step

D. Prazeres, J. Queiroz, F. Sousa, 2006, Biotechnology and applied biochemistry.

Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA.

D. Prazeres, J. Queiroz, F. Sousa, 2008, Trends in biotechnology.

Purification of supercoiled G-quadruplex pDNA for in vitro transcription

J. Queiroz, F. Sousa, Tiago Santos, 2016 .

Thermofluor-Based Optimization Strategy for the Stabilization of Recombinant Human Soluble Catechol-O-Methyltransferase

M. Romão, E. Gallardo, J. Queiroz, 2022, International journal of molecular sciences.

Development of a bioreactor system for cytotoxic evaluation of pharmacological compounds in living cells using NMR spectroscopy.

C. Geraldes, J. Queiroz, Josué Carvalho, 2019, Journal of pharmacological and toxicological methods.

Polymer-peptide ternary systems as a tool to improve the properties of plasmid DNA vectors in gene delivery

T. Albuquerque, A. R. Neves, D. Costa, 2020 .

Polyazamacrocycles as Potential Antitumor Agents for Human Prostate Cancer Cells

J. Queiroz, C. Cruz, E. Cairrão, 2013, Chemical biology & drug design.

A new insight in gellan microspheres application to capture a plasmid DNA vaccine from an Escherichia coli lysate

D. Costa, J. Queiroz, L. Passarinha, 2021 .

Study of hydrophobic interaction adsorption of bovine serum albumin under overloaded conditions using flow microcalorimetry.

N. Pinto, J. Queiroz, A. C. Dias-Cabral, 1999, Journal of chromatography. A.

Stimuli-responsive polyamine-DNA blend nanogels for co-delivery in cancer therapy.

A. Valente, D. Costa, J. Queiroz, 2015, Colloids and surfaces. B, Biointerfaces.

AS1411 derivatives as carriers of G-quadruplex ligands for cervical cancer cells.

G. Salgado, J. Mergny, J. Queiroz, 2019, International journal of pharmaceutics.

Advances in Membrane-Bound Catechol-O-Methyltransferase Stability Achieved Using a New Ionic Liquid-Based Storage Formulation

M. Romão, E. Gallardo, Â. Sousa, 2022, International journal of molecular sciences.

Ligand screening to pre-miRNA 149 G-quadruplex investigated by molecular dynamics

G. Salgado, J. Queiroz, F. Sousa, 2020, Journal of biomolecular structure & dynamics.

Sensitive detection of peptide-minicircle DNA interactions by surface plasmon resonance.

I. Correia, C. Pichon, V. Gaspar, 2013, Analytical chemistry.

Fluorescent light-up acridine orange derivatives bind and stabilize KRAS-22RT G-quadruplex.

G. Salgado, J. Mergny, J. Queiroz, 2018, Biochimie.

Analysis of phenylpiperazine-like stimulants in human hair as trimethylsilyl derivatives by gas chromatography-mass spectrometry.

J. Queiroz, M. López-Rivadulla, M. Barroso, 2010, Journal of chromatography. A.

Poly(2-ethyl-2-oxazoline)-PLA-g-PEI amphiphilic triblock micelles for co-delivery of minicircle DNA and chemotherapeutics.

Elisabete C. Costa, I. Correia, C. Pichon, 2014, Journal of controlled release : official journal of the Controlled Release Society.

Determination of biomarkers of tobacco smoke exposure in oral fluid using solid-phase extraction and gas chromatography-tandem mass spectrometry.

E. Gallardo, J. Queiroz, S. Ravara, 2012, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Advances in time course extracellular production of human pre-miR-29b from Rhodovulum sulfidophilum

J. Tomás, J. Queiroz, F. Sousa, 2016, Applied Microbiology and Biotechnology.

Analysis of nucleotides binding to chromatography supports provided by nuclear magnetic resonance spectroscopy.

E. Cabrita, J. Queiroz, C. Cruz, 2011, Journal of chromatography. A.

Folate-Targeted Multifunctional Amino Acid-Chitosan Nanoparticles for Improved Cancer Therapy

Elisabete C. Costa, I. Correia, C. Pichon, 2015, Pharmaceutical Research.

Determination of seven selected antipsychotic drugs in human plasma using microextraction in packed sorbent and gas chromatography–tandem mass spectrometry

E. Gallardo, J. Queiroz, B. M. Fonseca, 2013, Analytical and Bioanalytical Chemistry.

Studies on the chromatographic fractionation of Trichoderma reesei cellulases by hydrophobic interaction.

J. Queiroz, C. Tomaz, C T Tomaz, 1999, Journal of chromatography. A.

Protein purification by aminosquarylium cyanine dye-affinity chromatography.

J. Queiroz, F. Sousa, L. V. Reis, 2013, Biomedical chromatography : BMC.

Multifunctional nanocarriers for codelivery of nucleic acids and chemotherapeutics to cancer cells

Elisabete C. Costa, I. Correia, C. Pichon, 2016 .

Stabilization of novel immunoglobulin switch regions G-quadruplexes by naphthalene and quinoline-based ligands

G. Salgado, J. Mergny, E. Cabrita, 2016 .

Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography.

D. Prazeres, J. Queiroz, F. Sousa, 2007, Archives of biochemistry and biophysics.

A critical review of microextraction by packed sorbent as a sample preparation approach in drug bioanalysis.

João Queiroz, Gilberto Alves, Márcio Rodrigues, 2013, Bioanalysis.

Chapter 6 – Hydrophobic Interaction Chromatography

J. Queiroz, C. Tomaz, 2013 .

Hydrophobic interaction chromatography of proteins.

J. Queiroz, C. Tomaz, C T Tomaz, 2001, Journal of biotechnology.

Purification of supercoiled p53-encoding plasmid using an arginine-modified macroporous support.

Â. Sousa, J. Queiroz, F. Sousa, 2020, Journal of chromatography. A.

Optimization of supercoiled HPV-16 E6/E7 plasmid DNA purification with arginine monolith using design of experiments.

Â. Sousa, J. Queiroz, F. Sousa, 2015, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Pharmaceutical-grade pre-miR-29 purification using an agmatine monolithic support.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Journal of chromatography. A.

Purification of pre-miR-29 by arginine-affinity chromatography.

I. Correia, Â. Sousa, J. Queiroz, 2014, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Purification of human papillomavirus 16 E6/E7 plasmid deoxyribonucleic acid-based vaccine using an arginine modified monolithic support.

J. Queiroz, F. Sousa, A. Sousa, 2013, Journal of chromatography. A.

Differential interactions of plasmid DNA, RNA and genomic DNA with amino acid-based affinity matrices.

Angela Sousa, Fani Sousa, João A Queiroz, 2010, Journal of separation science.

Phenanthroline-bis-oxazole ligands for binding and stabilization of G-quadruplexes.

G. Salgado, J. Mergny, E. Cabrita, 2017, Biochimica et biophysica acta. General subjects.

Bioanalytical procedures and recent developments in the determination of opiates/opioids in human biological samples

E. Gallardo, J. Queiroz, M. López-Rivadulla, 2011, Analytical and bioanalytical chemistry.

Current technologies and considerations for drug bioanalysis in oral fluid.

E. Gallardo, J. Queiroz, M. Barroso, 2009, Bioanalysis.

The role of alternative specimens in toxicological analysis.

E. Gallardo, J. Queiroz, Eugenia Gallardo, 2008, Biomedical chromatography : BMC.

Chromatography of plasmid DNA.

M. M. Diogo, D. Prazeres, J. Queiroz, 2005, Journal of chromatography. A.

Application of ethylenediamine monolith to purify a hemagglutinin influenza deoxyribonucleic acid-based vaccine

J. Queiroz, F. Sousa, C. Tomaz, 2015 .

Advances in chromatographic supports for pharmaceutical-grade plasmid DNA purification.

Angela Sousa, Fani Sousa, João A Queiroz, 2012, Journal of separation science.

Mitochondrial Gene Therapy: Advances in Mitochondrial Gene Cloning, Plasmid Production, and Nanosystems Targeted to Mitochondria.

D. Costa, J. Queiroz, F. Sousa, 2017, Molecular pharmaceutics.

Development of mitochondrial targeting plasmid DNA nanoparticles: Characterization and in vitro studies

D. Costa, J. Queiroz, F. Sousa, 2015 .

Nucleolin as a potential biomarker for canine malignant neoplasia.

J. Queiroz, Josué Carvalho, Tiago Santos, 2020, Research in veterinary science.

Human Papillomavirus G-Rich Regions as Potential Antiviral Drug Targets.

G. Salgado, J. Mergny, C. Meyers, 2020, Nucleic acid therapeutics.

Non-coding RNAs: Emerging from the discovery to therapeutic applications.

C. Pichon, J. Queiroz, M. Riscado, 2021, Biochemical pharmacology.

Quantitative analysis of the interaction between l-methionine derivative and oligonucleotides.

J. Queiroz, F. Sousa, C. Cruz, 2015, Journal of biochemistry.

Cervical cancer and HPV infection: ongoing therapeutic research to counteract the action of E6 and E7 oncoproteins.

Â. Sousa, J. Queiroz, F. Sousa, 2019, Drug discovery today.

Trends in proteomic analysis of human vitreous humor samples

J. Queiroz, C. Tomaz, L. Passarinha, 2014, Electrophoresis.

Ribonucleic acid purification.

J. Queiroz, F. Sousa, R. Martins, 2014, Journal of chromatography. A.

A new strategy for RNA isolation from eukaryotic cells using arginine affinity chromatography.

J. Queiroz, F. Sousa, C. Maia, 2012, Journal of separation science.

Current progress on microRNAs‐based therapeutics in neurodegenerative diseases

J. Queiroz, F. Sousa, A. Figueiras, 2017, Wiley interdisciplinary reviews. RNA.

Vitreous humor in the pathologic scope: Insights from proteomic approaches

J. Queiroz, J. Monteiro, J. Queiroz, 2015, Proteomics. Clinical applications.

Purification of Histidine-Tagged Membrane-Bound Catechol-O-Methyltransferase from Detergent-Solubilized Pichia pastoris Membranes

J. Queiroz, A. Q. Pedro, L. Passarinha, 2018, Chromatographia.

An Improved HPLC Method for Quantification of Metanephrine with Coulometric Detection

E. Gallardo, J. Queiroz, A. Q. Pedro, 2014 .

Scale-up of hydrophobic interaction chromatography for the purification of a DNA vaccine against rabies

P. Perrin, N. Tordo, M. M. Diogo, 2000, Biotechnology Letters.

Production, purification and analysis of an experimental DNA vaccine against rabies

P. Perrin, N. Tordo, M. M. Diogo, 2001, The journal of gene medicine.

Rhodamine based plasmid DNA nanoparticles for mitochondrial gene therapy.

D. Costa, J. Queiroz, F. Sousa, 2014, Colloids and surfaces. B, Biointerfaces.

A novel prokaryotic expression system for biosynthesis of recombinant human membrane-bound catechol-O-methyltransferase.

J. Queiroz, A. Q. Pedro, M. Bonifácio, 2011, Journal of biotechnology.

LC-MS: a powerful tool in workplace drug testing.

E. Gallardo, J. Queiroz, M. Barroso, 2009, Drug testing and analysis.

Applications of gellan natural polymer microspheres in recombinant catechol-O-methyltransferase direct capture from a Komagataella pastoris lysate.

Â. Sousa, J. Queiroz, C. Gonçalves, 2021, International journal of biological macromolecules.

The effect of temperature on the analysis of metanephrine for catechol-O-methyltransferase activity assay by HPLC with electrochemical detection.

J. Queiroz, M. Bonifácio, L. Passarinha, 2006, Biomedical chromatography : BMC.

Role of microextraction sampling procedures in forensic toxicology.

E. Gallardo, J. Queiroz, M. Barroso, 2012, Bioanalysis.

Determination of piperazine-type stimulants in human urine by means of microextraction in packed sorbent and high performance liquid chromatography-diode array detection.

E. Gallardo, J. Queiroz, M. Barroso, 2012, Journal of pharmaceutical and biomedical analysis.

Effect of Chromatographic Conditions on Supercoiled Plasmid DNA Stability and Bioactivity

Â. Sousa, J. Queiroz, A. Q. Pedro, 2019 .

Optimization of peptide-plasmid DNA vectors formulation for gene delivery in cancer therapy exploring design of experiments.

P. Boisguérin, D. Costa, Â. Sousa, 2019, Colloids and surfaces. B, Biointerfaces.

Development of fed-batch profiles for efficient biosynthesis of catechol-O-methyltransferase

J. Queiroz, A. Q. Pedro, M. Bonifácio, 2014, Biotechnology reports.

Smart micelleplexes as a new therapeutic approach for RNA delivery

J. Queiroz, F. Sousa, F. Veiga, 2017, Expert opinion on drug delivery.

Minicircle DNA: The Future for DNA-Based Vectors?

Â. Sousa, J. Queiroz, F. Sousa, 2020, Trends in biotechnology.

Minicircle DNA vectors for gene therapy: advances and applications

Fani Sousa, Elisabete C. Costa, Chantal Pichon, 2015, Expert opinion on biological therapy.

DoE to improve supercoiled p53-pDNA purification by O-phospho-l-tyrosine chromatography.

Â. Sousa, J. Queiroz, F. Sousa, 2019, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Chromatographic HPV‐16 E6/E7 plasmid vaccine purification employing L‐histidine and 1‐benzyl‐L‐histidine affinity ligands

Â. Sousa, J. Queiroz, F. Sousa, 2017, Electrophoresis.

Selective purification of supercoiled p53-encoding pDNA with L-methionine-agarose matrix.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Analytical biochemistry.

Histamine monolith versatility to purify supercoiled plasmid deoxyribonucleic acid from Escherichia coli lysate.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Journal of chromatography. A.

Purification of pre-miR-29 by a new O-phospho-l-tyrosine affinity chromatographic strategy optimized using design of experiments.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Journal of chromatography. A.

Rapid quantification of supercoiled plasmid deoxyribonucleic acid using a monolithic ion exchanger.

Â. Sousa, J. Queiroz, F. Sousa, 2013, Journal of chromatography. A.

Impact of lysine-affinity chromatography on supercoiled plasmid DNA purification.

Â. Sousa, J. Queiroz, F. Sousa, 2011, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography

Fani Sousa, João A Queiroz, J. Queiroz, 2009, The journal of gene medicine.

The use of size-exclusion chromatography in the isolation of supercoiled minicircle DNA from Escherichia coli lysate.

Â. Sousa, J. Queiroz, F. Sousa, 2020, Journal of chromatography. A.

Quality assessment of supercoiled minicircle DNA by cadaverine-modified analytical chromatographic monolith.

Â. Sousa, J. Queiroz, F. Sousa, 2019, Journal of pharmaceutical and biomedical analysis.

Minicircle DNA purification: Performance of chromatographic monoliths bearing lysine and cadaverine ligands.

Â. Sousa, J. Queiroz, F. Sousa, 2019, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Minicircle DNA purification using a CIM® DEAE-1 monolithic support.

Â. Sousa, J. Queiroz, F. Sousa, 2016, Journal of separation science.

Improved minicircle DNA biosynthesis for gene therapy applications.

I. Correia, C. Pichon, V. Gaspar, 2014, Human gene therapy methods.

Simultaneous quantitation of morphine, 6-acetylmorphine, codeine, 6-acetylcodeine and tramadol in hair using mixed-mode solid-phase extraction and gas chromatography–mass spectrometry

J. Queiroz, M. López-Rivadulla, M. Barroso, 2010, Analytical and bioanalytical chemistry.

Development and validation of an analytical method for the simultaneous determination of cocaine and its main metabolite, benzoylecgonine, in human hair by gas chromatography/mass spectrometry.

J. Queiroz, M. López-Rivadulla, M. Barroso, 2008, Rapid communications in mass spectrometry : RCM.

Polyethylenimine coated plasmid DNA-surfactant complexes as potential gene delivery systems.

W. Briscoe, D. Costa, J. Queiroz, 2015, Colloids and surfaces. B, Biointerfaces.

Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines Field.

A. R. Neves, D. Costa, Â. Sousa, 2021, Methods in molecular biology.

Design of experiments to select triphenylphosphonium-polyplexes with suitable physicochemical properties for mitochondrial gene therapy

T. Albuquerque, S. Biswas, Himanshu S. Bhatt, 2020 .

A co-delivery platform based on plasmid DNA peptide-surfactant complexes: formation, characterization and release behavior.

T. Albuquerque, A. Valente, D. Costa, 2019, Colloids and surfaces. B, Biointerfaces.

Finding the ideal polyethylenimine-plasmid DNA system for co-delivery of payloads in cancer therapy.

A. Valente, D. Costa, Â. Sousa, 2018, Colloids and surfaces. B, Biointerfaces.

DNA-Based Hydrogels: An Approach for Multifunctional Bioapplications

A. Valente, D. Costa, J. Queiroz, 2018 .

Integrated Extraction-Preservation Strategies for RNA Using Biobased Ionic Liquids

J. Coutinho, M. Freire, J. Queiroz, 2019, ACS Sustainable Chemistry & Engineering.

Mixed-mode solid-phase extraction for sample cleanup in hair analysis for methadone and its main metabolite.

J. Queiroz, M. López-Rivadulla, M. Barroso, 2010, Biomedical chromatography : BMC.

Determination of eight selected organophosphorus insecticides in postmortem blood samples using solid-phase extraction and gas chromatography/mass spectrometry.

E. Gallardo, J. Queiroz, M. Barroso, 2010, Rapid communications in mass spectrometry : RCM.

Analytical approach to determine biogenic amines in urine using microextraction in packed syringe and liquid chromatography coupled to electrochemical detection.

E. Gallardo, J. Queiroz, L. Passarinha, 2013, Biomedical chromatography : BMC.

Recombinant pre-miR-29b for Alzheimer´s disease therapeutics

Fani Sousa, João A. Queiroz, J. Tomás, 2016, Scientific Reports.

New approach for purification of pre-miR-29 using lysine-affinity chromatography.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Journal of chromatography. A.

Enhanced biosynthesis of plasmid DNA from Escherichia coli VH33 using Box–Behnken design associated to aromatic amino acids pathway

J. Queiroz, F. Sousa, A. Q. Pedro, 2015 .

Effect of chromatographic conditions and plasmid DNA size on the dynamic binding capacity of a monolithic support.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Journal of Separation Science.

Purification of influenza deoxyribonucleic acid-based vaccine using agmatine monolith.

Â. Sousa, J. Queiroz, F. Sousa, 2016, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Screening of l‐histidine‐based ligands to modify monolithic supports and selectively purify the supercoiled plasmid DNA isoform

Â. Sousa, J. Queiroz, F. Sousa, 2015, Journal of molecular recognition : JMR.

Successful application of monolithic innovative technology using a carbonyldiimidazole disk to purify supercoiled plasmid DNA suitable for pharmaceutical applications.

Â. Sousa, J. Queiroz, F. Sousa, 2011, Journal of chromatography. A.

Selectivity of arginine chromatography in promoting different interactions using synthetic oligonucleotides as model.

Â. Sousa, J. Queiroz, F. Sousa, 2009, Journal of separation science.

Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine-agarose support.

D. Prazeres, J. Queiroz, F. Sousa, 2005, Analytical biochemistry.

Analysis of hSCOMT adsorption in bioaffinity chromatography with immobilized amino acids: the influence of pH and ionic strength.

J. Queiroz, M. Bonifácio, L. Passarinha, 2011, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Thiacarbocyanine as ligand in dye-affinity chromatography for protein purification. II. Dynamic binding capacity using lysozyme as a model.

J. Queiroz, F. Sousa, U. Anyanwu, 2009, Biomedical chromatography : BMC.

L-tryptophan and dipeptide derivatives for supercoiled plasmid DNA purification.

E. Cabrita, J. Queiroz, M. Corvo, 2016, International journal of biological macromolecules.

Plasmid DNA purification using a multimodal chromatography resin

João A Queiroz, Tiago Matos, Leif Bülow, 2014, Journal of molecular recognition : JMR.

Biorecognition of supercoiled plasmid DNA isoform in lysine-affinity chromatography.

Â. Sousa, J. Queiroz, F. Sousa, 2009, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

The biological performance of purified supercoiled p53 plasmid DNA in different cancer cell lines

V. Gaspar, J. Queiroz, F. Sousa, 2018, Process Biochemistry.

Analysis of pre-miR-29b binding conditions to amino acids by using a surface plasmon resonance biosensor

J. Queiroz, F. Sousa, A. Figueiras, 2016 .

Molecular recognition of oligonucleotides and plasmid DNA by l‐methionine

Â. Sousa, J. Queiroz, C. Cruz, 2014, Journal of molecular recognition : JMR.

Study of the specific interaction between L-methionine chromatography support and nucleotides.

Â. Sousa, J. Queiroz, F. Sousa, 2012, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention.

D. Prazeres, Â. Sousa, J. Queiroz, 2009, Biomedical chromatography : BMC.

Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography.

D. Prazeres, J. Queiroz, F. Sousa, 2008, Analytical biochemistry.

A new affinity approach to isolate Escherichia coli 6S RNA with histidine‐chromatography

J. Queiroz, F. Sousa, R. Martins, 2010, Journal of molecular recognition : JMR.

Formulation of chitosan–TPP–pDNA nanocapsules for gene therapy applications

I. Correia, V. Gaspar, J. Queiroz, 2011, Nanotechnology.

Analysis of Salvinorin A in urine using microextraction in packed syringe and GC-MS/MS.

E. Gallardo, J. Queiroz, A. Cruz, 2013, Bioanalysis.

Rapid determination of piperazine-type stimulants in human urine by microextraction in packed sorbent after method optimization using a multivariate approach.

E. Gallardo, J. Queiroz, M. Barroso, 2012, Journal of chromatography. A.

Brain-Targeted Delivery of Pre-miR-29b Using Lactoferrin-Stearic Acid-Modified-Chitosan/Polyethyleneimine Polyplexes

J. Tomás, J. Queiroz, F. Sousa, 2020, Pharmaceuticals.

p53 as the Focus of Gene Therapy: Past, Present and Future.

J. Queiroz, F. Sousa, J. Valente, 2018, Current drug targets.

A rapid HPLC method for the simultaneous determination of amiodarone and its major metabolite in rat plasma and tissues: a useful tool for pharmacokinetic studies.

J. Queiroz, A. Falcão, G. Alves, 2013, Journal of chromatographic science.

Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation

Â. Sousa, J. Queiroz, F. Sousa, 2021, Polymers.

Thiacarbocyanine as ligand in dye-affinity chromatography for protein purification.

J. Queiroz, R. Boto, P. Almeida, 2008, Biomedical chromatography : BMC.

Isolation of PCR DNA fragments using Aqueous Two-Phase Systems

J. Queiroz, L. Bülow, T. Matos, 2014 .

Arginine homopeptides for plasmid DNA purification using monolithic supports.

Â. Sousa, J. Queiroz, F. Sousa, 2018, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Nucleic acid and protein extraction from electropermeabilized E. coli cells on a microfluidic chip.

J. Kutter, L. Bulow, J. Queiroz, 2013, The Analyst.

Gel network photodisruption: a new strategy for the codelivery of plasmid DNA and drugs.

A. Valente, M. Miguel, D. Costa, 2011, Langmuir : the ACS journal of surfaces and colloids.

Influenza Plasmid DNA Vaccines: Progress and Prospects.

J. Queiroz, C. Tomaz, D. Bicho, 2015, Current gene therapy.

Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment.

Â. Sousa, J. Queiroz, F. Sousa, 2021, Methods in molecular biology.

Phthalocyanines for G-quadruplex aptamers binding.

G. Salgado, J. Mergny, M. Neves, 2020, Bioorganic chemistry.

Nanoaggregate-forming lipid-conjugated AS1411 aptamer AS a promising tumor-targeted delivery system of anticancer agents in vitro.

A. Ellington, G. Salgado, J. Mergny, 2021, Nanomedicine : nanotechnology, biology, and medicine.

Aptamer-based Targeted Delivery of a G-quadruplex Ligand in Cervical Cancer Cells

G. Salgado, J. Mergny, J. Queiroz, 2019, Scientific Reports.

Targeting of Cellular Organelles by Fluorescent Plasmid DNA Nanoparticles.

D. Costa, J. Queiroz, M. Caldeira, 2017, Biomacromolecules.

Studies on the retention of plasmid DNA and Escherichia coli nucleic acids by hydrophobic interaction chromatography

M. M. Diogo, D. Prazeres, J. Queiroz, 2001, Bioseparation.

New insights for therapeutic recombinant human miRNAs heterologous production: Rhodovolum sulfidophilum vs Escherichia coli

J. Queiroz, F. Sousa, A. Figueiras, 2017, Bioengineered.

Smoothing membrane protein structure determination by initial upstream stage improvements

J. Queiroz, A. Q. Pedro, L. Passarinha, 2019, Applied Microbiology and Biotechnology.

Plasmid production and purification: An integrated experiment‐based biochemistry and biotechnology laboratory course

J. Queiroz, F. Sousa, Tiago Santos, 2019, Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology.

RNA G-quadruplex as supramolecular carrier for cancer-selective delivery.

E. Cabrita, J. Queiroz, Tiago Santos, 2019, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

The Performance of Minicircle DNA Versus Parental Plasmid in p53 Gene Delivery Into HPV-18-Infected Cervical Cancer Cells.

Joel M. Alves, Â. Sousa, J. Queiroz, 2020, Nucleic acid therapeutics.

Ethylenediamine-Derived Chromatographic Ligand to Separate BSA, Lysozyme, and RNase A

J. Queiroz, F. Sousa, L. V. Reis, 2014, Chromatographia.

Study of specific interaction between nucleotides and dye support by nuclear magnetic resonance

J. Queiroz, C. Cruz, P. Almeida, 2011, Journal of molecular recognition : JMR.

NMR screening of new carbocyanine dyes as ligands for affinity chromatography.

J. Queiroz, C. Cruz, A. Drzazga, 2014, Journal of molecular recognition : JMR.

Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate

I. Correia, C. Pichon, V. Gaspar, 2017 .

Biosynthesis and purification of histidine‐tagged human soluble catechol‐O‐methyltransferase

J. Queiroz, A. Q. Pedro, M. Bonifácio, 2016 .

Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography

S. Martins, M. M. Diogo, J. Queiroz, 2000, Biotechnology and bioengineering.

Circular Dichroism of G-Quadruplex: A Laboratory Experiment for the Study of Topology and Ligand Binding.

J. Queiroz, Josué Carvalho, C. Cruz, 2017 .

p53-Encoding pDNA Purification by Affinity Chromatography for Cancer Therapy.

Â. Sousa, J. Queiroz, F. Sousa, 2015, Methods in molecular biology.

Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification.

D. Prazeres, J. Queiroz, F. Sousa, 2009, Biomedical chromatography : BMC.

Affinity analysis and application of dipeptides derived from l-tyrosine in plasmid purification.

E. Cabrita, J. Queiroz, F. Sousa, 2015, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Advances in time course extracellular production of human pre-miR-29b from Rhodovulum sulfidophilum

Fani Sousa, João A Queiroz, Ana Figueiras, 2016, Applied Microbiology and Biotechnology.

A rapid HPLC method for the simultaneous determination of amiodarone and its major metabolite in rat plasma and tissues: a useful tool for pharmacokinetic studies.

J. Queiroz, A. Falcão, G. Alves, 2013, Journal of chromatographic science.

Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography

J. Queiroz, F. Sousa, D. Prazeres, 2009, The journal of gene medicine.