F. Sousa

发表

Bioreducible poly(2-ethyl-2-oxazoline)-PLA-PEI-SS triblock copolymer micelles for co-delivery of DNA minicircles and Doxorubicin.

Elisabete C. Costa, I. Correia, C. Pichon, 2015, Journal of controlled release : official journal of the Controlled Release Society.

Molecular Beacon Assay Development for Severe Acute Respiratory Syndrome Coronavirus 2 Detection

F. Sousa, Josué Carvalho, Jéssica Lopes-Nunes, 2021, Sensors.

Performance of a non-grafted monolithic support for purification of supercoiled plasmid DNA.

Â. Sousa, J. Queiroz, F. Sousa, 2011, Journal of chromatography. A.

Enhancement of a biotechnological platform for the purification and delivery of a human papillomavirus supercoiled plasmid DNA vaccine.

D. Costa, Â. Sousa, J. Queiroz, 2020, New biotechnology.

Pichia pastoris: a recombinant microfactory for antibodies and human membrane proteins.

J. Queiroz, F. Sousa, A. Q. Pedro, 2013, Journal of microbiology and biotechnology.

Development of a model for membrane filtration of long and flexible macromolecules: Application to predict dextran and linear DNA rejections in ultrafiltration

A. Morão, J. Queiroz, F. Sousa, 2009 .

Folate-Targeted Multifunctional Amino Acid-Chitosan Nanoparticles for Improved Cancer Therapy

Elisabete C. Costa, I. Correia, C. Pichon, 2014, Pharmaceutical Research.

Nanoparticle mediated delivery of pure P53 supercoiled plasmid DNA for gene therapy.

I. Correia, Â. Sousa, V. Gaspar, 2011, Journal of controlled release : official journal of the Controlled Release Society.

Supercoiled plasmid quality assessment by analytical arginine-affinity chromatography.

J. Queiroz, F. Sousa, 2011, Journal of chromatography. A.

Biomedical application of plasmid DNA in gene therapy: A new challenge for chromatography

J. Queiroz, F. Sousa, L. Passarinha, 2009, Biotechnology & genetic engineering reviews.

Naphthalene amine support for G-quadruplex isolation.

J. Queiroz, F. Sousa, M. Corvo, 2017, The Analyst.

Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography.

D. Prazeres, J. Queiroz, F. Sousa, 2007, Biomedical chromatography : BMC.

Development of plasmid DNA nanoparticles for mitochondrial gene therapy

D. Costa, J. Queiroz, F. Sousa, 2019, Materials for Biomedical Engineering.

Affinity approaches in RNAi-based therapeutics purification.

J. Queiroz, F. Sousa, A. Figueiras, 2016, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

HPV‐16 targeted DNA vaccine expression: The role of purification

J. Tomás, Â. Sousa, J. Queiroz, 2018, Biotechnology progress.

Histidine affinity chromatography-based methodology for the simultaneous isolation of Escherichia coli small and ribosomal RNA.

J. Queiroz, F. Sousa, R. Martins, 2012, Biomedical chromatography : BMC.

Advances Brought by Hydrophilic Ionic Liquids in Fields Involving Pharmaceuticals

M. Freire, F. Sousa, Teresa B. V. Dinis, 2021, Materials.

Insights on the DNA Stability in Aqueous Solutions of Ionic Liquids

M. Freire, F. Sousa, Teresa B. V. Dinis, 2020, Frontiers in Bioengineering and Biotechnology.

Dilemma on plasmid DNA purification: binding capacity vs selectivity.

J. Queiroz, F. Sousa, J. Valente, 2020, Journal of chromatography. A.

Gas-generating TPGS-PLGA microspheres loaded with nanoparticles (NIMPS) for co-delivery of minicircle DNA and anti-tumoral drugs.

Elisabete C. Costa, I. Correia, C. Pichon, 2015, Colloids and surfaces. B, Biointerfaces.

Influence of growth conditions on plasmid DNA production.

J. Queiroz, F. Domingues, F. Sousa, 2009, Journal of microbiology and biotechnology.

Binding mechanisms for histamine and agmatine ligands in plasmid deoxyribonucleic acid purifications.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Journal of chromatography. A.

Biosynthesis of small noncoding RNAs in Rhodovulum sulfidophilum: optimization of the fermentation process

F. Sousa, Resumo Alargado, D. Interior, 2018 .

Thermodynamic study of the interaction between linear plasmid DNA and an anion exchange support under linear and overloaded conditions.

F. Sousa, A. C. Dias-Cabral, P. Aguilar, 2014, Journal of chromatography. A.

Amino acid derivatized monoliths for purification of a DNA vaccine against influenza

J. Queiroz, F. Sousa, C. Tomaz, 2015, 2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG).

Selective purification of supercoiled plasmid DNA from clarified cell lysates with a single histidine–agarose chromatography step

D. Prazeres, J. Queiroz, F. Sousa, 2006, Biotechnology and applied biochemistry.

Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA.

D. Prazeres, J. Queiroz, F. Sousa, 2008, Trends in biotechnology.

Purification of supercoiled G-quadruplex pDNA for in vitro transcription

J. Queiroz, F. Sousa, Tiago Santos, 2016 .

Supported Ionic Liquids Used as Chromatographic Matrices in Bioseparation—An Overview

M. C. Neves, M. Freire, F. Sousa, 2022, Molecules.

Molecular Beacon Assay Development for Severe Acute Respiratory Syndrome Coronavirus 2 Detection

F. Sousa, Josué Carvalho, Jéssica Lopes-Nunes, 2021, Sensors.

Preparation of well-defined brush-like block copolymers for gene delivery applications under biorelevant reaction conditions.

A. Serra, J. Coelho, J. Góis, 2018, Colloids and surfaces. B, Biointerfaces.

Ligand screening to pre-miRNA 149 G-quadruplex investigated by molecular dynamics

G. Salgado, J. Queiroz, F. Sousa, 2020, Journal of biomolecular structure & dynamics.

Sensitive detection of peptide-minicircle DNA interactions by surface plasmon resonance.

I. Correia, C. Pichon, V. Gaspar, 2013, Analytical chemistry.

Poly(2-ethyl-2-oxazoline)-PLA-g-PEI amphiphilic triblock micelles for co-delivery of minicircle DNA and chemotherapeutics.

Elisabete C. Costa, I. Correia, C. Pichon, 2014, Journal of controlled release : official journal of the Controlled Release Society.

Advances in time course extracellular production of human pre-miR-29b from Rhodovulum sulfidophilum

J. Tomás, J. Queiroz, F. Sousa, 2016, Applied Microbiology and Biotechnology.

Folate-Targeted Multifunctional Amino Acid-Chitosan Nanoparticles for Improved Cancer Therapy

Elisabete C. Costa, I. Correia, C. Pichon, 2015, Pharmaceutical Research.

Protein purification by aminosquarylium cyanine dye-affinity chromatography.

J. Queiroz, F. Sousa, L. V. Reis, 2013, Biomedical chromatography : BMC.

Multifunctional nanocarriers for codelivery of nucleic acids and chemotherapeutics to cancer cells

Elisabete C. Costa, I. Correia, C. Pichon, 2016 .

Stabilization of novel immunoglobulin switch regions G-quadruplexes by naphthalene and quinoline-based ligands

G. Salgado, J. Mergny, E. Cabrita, 2016 .

Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography.

D. Prazeres, J. Queiroz, F. Sousa, 2007, Archives of biochemistry and biophysics.

Purification of supercoiled p53-encoding plasmid using an arginine-modified macroporous support.

Â. Sousa, J. Queiroz, F. Sousa, 2020, Journal of chromatography. A.

Optimization of supercoiled HPV-16 E6/E7 plasmid DNA purification with arginine monolith using design of experiments.

Â. Sousa, J. Queiroz, F. Sousa, 2015, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Pharmaceutical-grade pre-miR-29 purification using an agmatine monolithic support.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Journal of chromatography. A.

Purification of pre-miR-29 by arginine-affinity chromatography.

I. Correia, Â. Sousa, J. Queiroz, 2014, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Purification of human papillomavirus 16 E6/E7 plasmid deoxyribonucleic acid-based vaccine using an arginine modified monolithic support.

J. Queiroz, F. Sousa, A. Sousa, 2013, Journal of chromatography. A.

Differential interactions of plasmid DNA, RNA and genomic DNA with amino acid-based affinity matrices.

Angela Sousa, Fani Sousa, João A Queiroz, 2010, Journal of separation science.

Application of ethylenediamine monolith to purify a hemagglutinin influenza deoxyribonucleic acid-based vaccine

J. Queiroz, F. Sousa, C. Tomaz, 2015 .

Advances in chromatographic supports for pharmaceutical-grade plasmid DNA purification.

Angela Sousa, Fani Sousa, João A Queiroz, 2012, Journal of separation science.

mRNA, a Revolution in Biomedicine

C. Pichon, F. Sousa, Nabila Laroui, 2021, Pharmaceutics.

Mitochondrial Gene Therapy: Advances in Mitochondrial Gene Cloning, Plasmid Production, and Nanosystems Targeted to Mitochondria.

D. Costa, J. Queiroz, F. Sousa, 2017, Molecular pharmaceutics.

Development of mitochondrial targeting plasmid DNA nanoparticles: Characterization and in vitro studies

D. Costa, J. Queiroz, F. Sousa, 2015 .

New RNA-Based Breakthroughs in Alzheimer’s Disease Diagnosis and Therapeutics

F. Sousa, M. Riscado, Bruno Baptista, 2021, Pharmaceutics.

Quantitative analysis of the interaction between l-methionine derivative and oligonucleotides.

J. Queiroz, F. Sousa, C. Cruz, 2015, Journal of biochemistry.

Cervical cancer and HPV infection: ongoing therapeutic research to counteract the action of E6 and E7 oncoproteins.

Â. Sousa, J. Queiroz, F. Sousa, 2019, Drug discovery today.

Efficient Isolation of Bacterial RNAs Using Silica-Based Materials Modified with Ionic Liquids

M. C. Neves, M. Freire, F. Sousa, 2021, Life.

Ribonucleic acid purification.

J. Queiroz, F. Sousa, R. Martins, 2014, Journal of chromatography. A.

A new strategy for RNA isolation from eukaryotic cells using arginine affinity chromatography.

J. Queiroz, F. Sousa, C. Maia, 2012, Journal of separation science.

Current progress on microRNAs‐based therapeutics in neurodegenerative diseases

J. Queiroz, F. Sousa, A. Figueiras, 2017, Wiley interdisciplinary reviews. RNA.

Rhodamine based plasmid DNA nanoparticles for mitochondrial gene therapy.

D. Costa, J. Queiroz, F. Sousa, 2014, Colloids and surfaces. B, Biointerfaces.

Ultrafiltration of supercoiled plasmid DNA : modeling and application

Fani Sousa, João A. Queiroz, Isabel C. Escobar, 2011 .

Understanding the adsorption of plasmid DNA and RNA molecules onto arginine-agarose chromatographic resin

F. Sousa, P. A. P. Pessôa Filho, Sara Cardoso, 2022, Molecular Biology Reports.

Characterization of polyplexes involving small RNA.

A. Jorge, A. Pais, F. Sousa, 2012, Journal of colloid and interface science.

Smart micelleplexes as a new therapeutic approach for RNA delivery

J. Queiroz, F. Sousa, F. Veiga, 2017, Expert opinion on drug delivery.

Minicircle DNA: The Future for DNA-Based Vectors?

Â. Sousa, J. Queiroz, F. Sousa, 2020, Trends in biotechnology.

Minicircle DNA vectors for gene therapy: advances and applications

Fani Sousa, Elisabete C. Costa, Chantal Pichon, 2015, Expert opinion on biological therapy.

DoE to improve supercoiled p53-pDNA purification by O-phospho-l-tyrosine chromatography.

Â. Sousa, J. Queiroz, F. Sousa, 2019, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Chromatographic HPV‐16 E6/E7 plasmid vaccine purification employing L‐histidine and 1‐benzyl‐L‐histidine affinity ligands

Â. Sousa, J. Queiroz, F. Sousa, 2017, Electrophoresis.

Selective purification of supercoiled p53-encoding pDNA with L-methionine-agarose matrix.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Analytical biochemistry.

Histamine monolith versatility to purify supercoiled plasmid deoxyribonucleic acid from Escherichia coli lysate.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Journal of chromatography. A.

Purification of pre-miR-29 by a new O-phospho-l-tyrosine affinity chromatographic strategy optimized using design of experiments.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Journal of chromatography. A.

Rapid quantification of supercoiled plasmid deoxyribonucleic acid using a monolithic ion exchanger.

Â. Sousa, J. Queiroz, F. Sousa, 2013, Journal of chromatography. A.

Impact of lysine-affinity chromatography on supercoiled plasmid DNA purification.

Â. Sousa, J. Queiroz, F. Sousa, 2011, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography

Fani Sousa, João A Queiroz, J. Queiroz, 2009, The journal of gene medicine.

The use of size-exclusion chromatography in the isolation of supercoiled minicircle DNA from Escherichia coli lysate.

Â. Sousa, J. Queiroz, F. Sousa, 2020, Journal of chromatography. A.

Quality assessment of supercoiled minicircle DNA by cadaverine-modified analytical chromatographic monolith.

Â. Sousa, J. Queiroz, F. Sousa, 2019, Journal of pharmaceutical and biomedical analysis.

Minicircle DNA purification: Performance of chromatographic monoliths bearing lysine and cadaverine ligands.

Â. Sousa, J. Queiroz, F. Sousa, 2019, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Minicircle DNA purification using a CIM® DEAE-1 monolithic support.

Â. Sousa, J. Queiroz, F. Sousa, 2016, Journal of separation science.

Improved minicircle DNA biosynthesis for gene therapy applications.

I. Correia, C. Pichon, V. Gaspar, 2014, Human gene therapy methods.

Integrated Extraction-Preservation Strategies for RNA Using Biobased Ionic Liquids

J. Coutinho, M. Freire, J. Queiroz, 2019, ACS Sustainable Chemistry & Engineering.

Recombinant pre-miR-29b for Alzheimer´s disease therapeutics

Fani Sousa, João A. Queiroz, J. Tomás, 2016, Scientific Reports.

New approach for purification of pre-miR-29 using lysine-affinity chromatography.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Journal of chromatography. A.

Enhanced biosynthesis of plasmid DNA from Escherichia coli VH33 using Box–Behnken design associated to aromatic amino acids pathway

J. Queiroz, F. Sousa, A. Q. Pedro, 2015 .

Effect of chromatographic conditions and plasmid DNA size on the dynamic binding capacity of a monolithic support.

Â. Sousa, J. Queiroz, F. Sousa, 2014, Journal of Separation Science.

Purification of influenza deoxyribonucleic acid-based vaccine using agmatine monolith.

Â. Sousa, J. Queiroz, F. Sousa, 2016, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Screening of l‐histidine‐based ligands to modify monolithic supports and selectively purify the supercoiled plasmid DNA isoform

Â. Sousa, J. Queiroz, F. Sousa, 2015, Journal of molecular recognition : JMR.

Supercoiled plasmid DNA purification by integrating membrane technology with a monolithic chromatography.

Angela Sousa, Fani Sousa, João A Queiroz, 2014, Journal of separation science.

Successful application of monolithic innovative technology using a carbonyldiimidazole disk to purify supercoiled plasmid DNA suitable for pharmaceutical applications.

Â. Sousa, J. Queiroz, F. Sousa, 2011, Journal of chromatography. A.

Selectivity of arginine chromatography in promoting different interactions using synthetic oligonucleotides as model.

Â. Sousa, J. Queiroz, F. Sousa, 2009, Journal of separation science.

Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine-agarose support.

D. Prazeres, J. Queiroz, F. Sousa, 2005, Analytical biochemistry.

Thiacarbocyanine as ligand in dye-affinity chromatography for protein purification. II. Dynamic binding capacity using lysozyme as a model.

J. Queiroz, F. Sousa, U. Anyanwu, 2009, Biomedical chromatography : BMC.

Biorecognition of supercoiled plasmid DNA isoform in lysine-affinity chromatography.

Â. Sousa, J. Queiroz, F. Sousa, 2009, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

The biological performance of purified supercoiled p53 plasmid DNA in different cancer cell lines

V. Gaspar, J. Queiroz, F. Sousa, 2018, Process Biochemistry.

Analysis of pre-miR-29b binding conditions to amino acids by using a surface plasmon resonance biosensor

J. Queiroz, F. Sousa, A. Figueiras, 2016 .

Study of the specific interaction between L-methionine chromatography support and nucleotides.

Â. Sousa, J. Queiroz, F. Sousa, 2012, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention.

D. Prazeres, Â. Sousa, J. Queiroz, 2009, Biomedical chromatography : BMC.

Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography.

D. Prazeres, J. Queiroz, F. Sousa, 2008, Analytical biochemistry.

Brain-Targeted Delivery of Pre-miR-29b Using Lactoferrin-Stearic Acid-Modified-Chitosan/Polyethyleneimine Polyplexes

J. Tomás, J. Queiroz, F. Sousa, 2020, Pharmaceuticals.

p53 as the Focus of Gene Therapy: Past, Present and Future.

J. Queiroz, F. Sousa, J. Valente, 2018, Current drug targets.

Effect of Plasmid DNA Size on Chitosan or Polyethyleneimine Polyplexes Formulation

Â. Sousa, J. Queiroz, F. Sousa, 2021, Polymers.

Arginine homopeptides for plasmid DNA purification using monolithic supports.

Â. Sousa, J. Queiroz, F. Sousa, 2018, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment.

Â. Sousa, J. Queiroz, F. Sousa, 2021, Methods in molecular biology.

New insights for therapeutic recombinant human miRNAs heterologous production: Rhodovolum sulfidophilum vs Escherichia coli

J. Queiroz, F. Sousa, A. Figueiras, 2017, Bioengineered.

Plasmid production and purification: An integrated experiment‐based biochemistry and biotechnology laboratory course

J. Queiroz, F. Sousa, Tiago Santos, 2019, Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology.

Integrated platform resorting to ionic liquids comprising the extraction, purification and preservation of DNA

M. Freire, F. Sousa, Teresa B. V. Dinis, 2023, Separation and Purification Technology.

Arginine-Modified 3D-Printed Chromatographic Supports

J. R. Dias, F. Sousa, N. Alves, 2022, Pharmaceutics.

The Performance of Minicircle DNA Versus Parental Plasmid in p53 Gene Delivery Into HPV-18-Infected Cervical Cancer Cells.

Joel M. Alves, Â. Sousa, J. Queiroz, 2020, Nucleic acid therapeutics.

Ethylenediamine-Derived Chromatographic Ligand to Separate BSA, Lysozyme, and RNase A

J. Queiroz, F. Sousa, L. V. Reis, 2014, Chromatographia.

Protein purification by aminosquarylium cyanine dye-affinity chromatography.

F. Sousa, P. Almeida, P. F. Santos, 2015, Methods in molecular biology.

Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate

I. Correia, C. Pichon, V. Gaspar, 2017 .

p53-Encoding pDNA Purification by Affinity Chromatography for Cancer Therapy.

Â. Sousa, J. Queiroz, F. Sousa, 2015, Methods in molecular biology.

Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification.

D. Prazeres, J. Queiroz, F. Sousa, 2009, Biomedical chromatography : BMC.

Formulation of chitosan–TPP–pDNA nanocapsules for gene therapy applications

I. Correia, V. Gaspar, J. Queiroz, 2011, Nanotechnology.

Affinity analysis and application of dipeptides derived from l-tyrosine in plasmid purification.

E. Cabrita, J. Queiroz, F. Sousa, 2015, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

Pristine Multi-walled Carbon Nanotubes for a rapid and efficient plasmid DNA clarification

M. Freire, F. Sousa, J. Faria, 2023, Separation and Purification Technology.

Amino acids–nucleotides biomolecular recognition: from biological occurrence to affinity chromatography

J. Queiroz, F. Sousa, C. Cruz, 2010, Journal of molecular recognition : JMR.

Advances in time course extracellular production of human pre-miR-29b from Rhodovulum sulfidophilum

Fani Sousa, João A Queiroz, Ana Figueiras, 2016, Applied Microbiology and Biotechnology.

A new affinity approach to isolate Escherichia coli 6S RNA with histidine‐chromatography

F. Sousa, R. Martins, João A. Queiroz, 2010, Journal of molecular recognition : JMR.

Multimodal ionic liquid-based chromatographic supports for an effective RNA purification

M. C. Neves, M. Freire, F. Sousa, 2023, Separation and Purification Technology.