P. Champeil

发表

Interaction of membrane proteins and lipids with solubilizing detergents.

J. Møller, M. le Maire, P. Champeil, 2000, Biochimica et biophysica acta.

Crystal Structure of D351A and P312A Mutant Forms of the Mammalian Sarcoplasmic Reticulum Ca2+-ATPase Reveals Key Events in Phosphorylation and Ca2+ Release*

P. Nissen, C. Jaxel, C. Olesen, 2008, Journal of Biological Chemistry.

Ca2+ versus Mg2+ coordination at the nucleotide-binding site of the sarcoplasmic reticulum Ca2+-ATPase.

P. Nissen, M. Picard, J. Møller, 2007, Journal of molecular biology.

Conformational changes in sarcoplasmic reticulum Ca(2+)-ATPase mutants: effect of mutations either at Ca(2+)-binding site II or at tryptophan 552 in the cytosolic domain.

C. Jaxel, M. Picard, M. le Maire, 2006, Biochemistry.

The Average Conformation at Micromolar [Ca2+] of Ca2+-ATPase with Bound Nucleotide Differs from That Adopted with the Transition State Analog ADP·AlFx or with AMPPCP under Crystallization Conditions at Millimolar [Ca2+]*

M. Picard, C. Toyoshima, P. Champeil, 2005, Journal of Biological Chemistry.

Involvement of the L6–7 Loop in SERCA1a Ca2+-ATPase Activation by Ca2+ (or Sr2+) and ATP*

M. Picard, J. Møller, M. le Maire, 2004, Journal of Biological Chemistry.

Proteolytic Studies on the Transduction Mechanism of Sarcoplasmic Reticulum Ca2+‐ATPase

P. Champeil, M. Maire, G. Lenoir, 2003, Annals of the New York Academy of Sciences.

A Remarkably Stable Phosphorylated Form of Ca2+-ATPase Prepared from Ca2+-loaded and Fluorescein Isothiocyanate-labeled Sarcoplasmic Reticulum Vesicles*

J. Lacapère, P. Champeil, D. McIntosh, 2001, The Journal of Biological Chemistry.

Phosphorylated Ca2+-ATPase Stable Enough for Structural Studies*

J. Lacapère, F. Delavoie, P. Champeil, 2001, The Journal of Biological Chemistry.

Tryptophan octyl ester in detergent micelles of dodecylmaltoside: fluorescence properties and quenching by brominated detergent analogs.

P. Champeil, M. Vincent, J. Gallay, 1999, Biophysical journal.

Brominated detergents as tools to study protein-detergent interactions.

D. Plusquellec, M. le Maire, P. Champeil, 1996, European journal of biochemistry.

Kinetic characterization of the perturbation by dodecylmaltoside of sarcoplasmic reticulum Ca(2+)-ATPase.

P. Champeil, F. Henao, B. de Foresta, 1992, European journal of biochemistry.

Cancellation of the cooperativity of Ca2+ binding to sarcoplasmic reticulum Ca(2+)-ATPase by the non-ionic detergent dodecylmaltoside.

P. Champeil, F. Henao, B. de Foresta, 1994, European journal of biochemistry.

Perturbation of the structure and function of a membranous Ca2+-ATPase by non-solubilizing concentrations of a non-ionic detergent.

J. Møller, M. le Maire, P. Champeil, 1983, European journal of biochemistry.

Inhibition by 4-hydroxynonenal (HNE) of Ca2+ transport by SERCA1a: low concentrations of HNE open protein-mediated leaks in the membrane.

P. Champeil, Solenne Chardonnet, C. Montigny, 2011, Free radical biology & medicine.

Binding of a nonionic detergent to membranes: flip-flop rate and location on the bilayer.

J. Møller, M. le Maire, P. Champeil, 1987, Biochemistry.

Inhibitors bound to Ca(2+)-free sarcoplasmic reticulum Ca(2+)-ATPase lock its transmembrane region but not necessarily its cytosolic region, revealing the flexibility of the loops connecting transmembrane and cytosolic domains.

M. Picard, C. Toyoshima, Carole Gauron, 2007, Biochemistry.

Kinetics of calcium dissociation from its high-affinity transport sites on sarcoplasmic reticulum ATPase.

S. Orlowski, P. Champeil, 1991, Biochemistry.

Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop

M. Picard, Carole Gauron, J. Møller, 2019, PloS one.

Slow Phospholipid Exchange between a Detergent-Solubilized Membrane Protein and Lipid-Detergent Mixed Micelles: Brominated Phospholipids as Tools to Follow Its Kinetics

Carole Gauron, S. Orlowski, J. Møller, 2017, PloS one.

A robust method to screen detergents for membrane protein stabilization, revisited.

S. Orlowski, J. Møller, M. le Maire, 2016, Analytical biochemistry.

Protein-protein contacts in solubilized membrane proteins, as detected by cross-linking.

J. Møller, J. Velours, M. le Maire, 2007, Analytical biochemistry.

Interaction of Amphipols with Sarcoplasmic Reticulum Ca2+-ATPase*

C. Tribet, J. Popot, M. le Maire, 2000, The Journal of Biological Chemistry.

Membrane solubilization by detergent: use of brominated phospholipids to evaluate the detergent-induced changes in Ca2+-ATPase/lipid interaction.

S. Orlowski, J. Møller, A. Lee, 1989, Biochemistry.

Does a radiolabelled ligand bind to a homogeneous population of non-interacting receptor sites?

S. Swillens, P. Champeil, M. Waelbroeck, 1995, Trends in pharmacological sciences.

Phosphoenzymes formed from Mg.ATP and Ca.ATP during pre-steady state kinetics of sarcoplasmic reticulum ATPase.

S. Orlowski, J. Møller, P. Champeil, 1988, The Journal of biological chemistry.

Kinetic characterization of the normal and detergent-perturbed reaction cycles of the sarcoplasmic reticulum calcium pump. Rate-limiting step(s) under different conditions.

J. Møller, M. le Maire, P. Champeil, 1986, The Journal of biological chemistry.

Ligand binding to macromolecules or micelles: use of centrifugal ultrafiltration to measure low-affinity binding.

M. le Maire, P. Champeil, P. Falson, 1998, Analytical biochemistry.

Pressure-induced inactivation of sarcoplasmic reticulum adenosine triphosphatase during high-speed centrifugation.

P. Champeil, F. Guillain, S. Bueschlen, 1981, Biochemistry.

Detection of Ca(2+)-binding proteins by electrophoretic migration in the presence of Ca2+ combined with 45Ca2+ overlay of protein blots.

S. Deschamps, J. Møller, M. le Maire, 1991, Analytical biochemistry.

Metal binding to ligands: cadmium complexes with glutathione revisited.

P. Champeil, P. Leverrier, C. Montigny, 2007, Analytical biochemistry.

Coordinated Overexpression in Yeast of a P4-ATPase and Its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex.

P. Champeil, R. Barry, G. Lenoir, 2016, Methods in molecular biology.

Different classes of tryptophan residues involved in the conformational changes characteristic of the sarcoplasmic reticulum Ca2(+)-ATPase cycle.

M. le Maire, P. Champeil, B. de Foresta, 1990, European journal of biochemistry.

On the molecular mechanism of flippase- and scramblase-mediated phospholipid transport.

J. Lyons, P. Nissen, P. Champeil, 2016, Biochimica et biophysica acta.

Calcium translocation mechanism in sarcoplasmic reticulum vesicles, deduced from location studies of protein-bound spin labels.

P. Champeil, J. Rigaud, C. Gary-Bobo, 1980, Proceedings of the National Academy of Sciences of the United States of America.

Amphipols from A to Z.

E. Goormaghtigh, W. Kühlbrandt, Y. Gohon, 2011, Annual review of biophysics.

Characterization of a Protease-resistant Domain of the Cytosolic Portion of Sarcoplasmic Reticulum Ca2+-ATPase

J. Møller, M. le Maire, P. Champeil, 1998, The Journal of Biological Chemistry.

Kinetic characterization of sarcoplasmic reticulum Ca2+-ATPase

P. Champeil, 1996 .

Mechanism of inhibition of the (Ca2(+)-Mg2+)-ATPase by nonylphenol.

S. Orlowski, P. Champeil, J. East, 1990, Biochemistry.

Kinetic characterization of the normal and procaine-perturbed reaction cycles of the sarcoplasmic reticulum calcium pump.

A. Cuenda, S. Orlowski, P. Champeil, 1991, European journal of biochemistry.

Nucleotide Binding to Na,K-ATPase: The Role of Electrostatic Interactions.

P. Champeil, N. Fedosova, M. Esmann, 2002, Biochemistry.

Overexpression of Membrane Proteins in Saccharomyces cerevisiae for Structural and Functional Studies: A Focus on the Rabbit Ca2+-ATPase Serca1a and on the Yeast Lipid “Flippase” Complex Drs2p/Cdc50p

C. Jaxel, P. Champeil, M. Maire, 2014 .

Conformational properties of some viral DNAs.

J. Brahms, P. Champeil, 1974, European journal of biochemistry.

Unexpected phosphoryl transfer from Asp351 to fluorescein attached to Lys515 in sarcoplasmic reticulum Ca2+-ATPase.

P. Champeil, C. Montigny, D. Mcintosh, 2008, Biochemistry.

Strontium binding to sarcoplasmic reticulum Ca(2+)-ATPase. Spectroscopic differentiation of the substeps involved.

S. Orlowski, P. Champeil, 1993, FEBS letters.

The Cytoplasmic Loop between Putative Transmembrane Segments 6 and 7 in Sarcoplasmic Reticulum Ca2+-ATPase Binds Ca2+ and Is Functionally Important*

L. Bouneau, J. Møller, M. le Maire, 1997, The Journal of Biological Chemistry.

Protective and inhibitory effects of various types of amphipols on the Ca2+-ATPase from sarcoplasmic reticulum: a comparative study.

J. Popot, F. Giusti, T. Dahmane, 2006, Biochemistry.

A new approach to the characterization of the B and A forms of DNA by I.R. spectroscopy.

J. Brahms, P. Champeil, T. Tran, 1973, Biochemical and biophysical research communications.

The calcium-sensitive dye arsenazo III inhibits calcium transport and ATP hydrolysis by the sarcoplasmic reticulum calcium pump.

P. Champeil, S. Riollet, 1987, Analytical biochemistry.

Sarcoplasmic reticulum ATPase. Spin labeling detection of ligand-induced changes in the relative reactivities of certain sulfhydryl groups.

P. Champeil, C. Gary-Bobo, F. Bastide, 1978, The Journal of biological chemistry.

Interaction of magnesium and inorganic phosphate with calcium-deprived sarcoplasmic reticulum adenosinetriphosphatase as reflected by organic solvent induced perturbation.

P. Champeil, F. Guillain, C. Vénien, 1985, Biochemistry.

Structural Studies of a Stabilized Phosphoenzyme Intermediate of Ca2+-ATPase*

D. Stokes, W. Rice, F. Delavoie, 2005, Journal of Biological Chemistry.

Effect of magnesium on the calcium-dependent transient kinetics of sarcoplasmic reticulum ATPase, studied by stopped flow fluorescence and phosphorylation.

P. Champeil, F. Guillain, G. Inesi, 1983, The Journal of biological chemistry.

Direct fluorescence measurements of Mg2+ binding to sarcoplasmic reticulum ATPase.

P. Champeil, F. Guillain, M. Gingold, 1982, The Journal of biological chemistry.

Glycyl betaine is effective in slowing down the irreversible denaturation of a detergent-solubilized membrane protein, sarcoplasmic reticulum Ca2+-ATPase (SERCA1a).

P. Champeil, B. Arnou, C. Montigny, 2010, Biochemical and biophysical research communications.

Rapid filtration study of the phosphorylation-dependent dissociation of calcium from transport sites of purified sarcoplasmic reticulum ATPase and ATP modulation of the catalytic cycle.

P. Champeil, F. Guillain, 1986, Biochemistry.

The Cytoplasmic Loop Located between Transmembrane Segments 6 and 7 Controls Activation by Ca2+ of Sarcoplasmic Reticulum Ca2+-ATPase*

S. Deschamps, L. Bouneau, J. Møller, 1998, The Journal of Biological Chemistry.

The two calcium ions initially bound to nonphosphorylated sarcoplasmic reticulum Ca(2+)-ATPase can no longer be kinetically distinguished when they dissociate from phosphorylated ATPase toward the lumen.

S. Orlowski, P. Champeil, 1991, Biochemistry.

Effects of phospholipids on binding of calcium to (Ca2(+)-Mg2(+)-ATPase.

S. Orlowski, P. Champeil, J. East, 1990, Biochemistry.

Phosphatidylserine Stimulation of Drs2p·Cdc50p Lipid Translocase Dephosphorylation Is Controlled by Phosphatidylinositol-4-phosphate*

C. Jaxel, M. le Maire, P. Champeil, 2012, The Journal of Biological Chemistry.

Sarcoplasmic reticulum adenosinetriphosphatase phosphorylation from inorganic phosphate. Theoretical and experimental reinvestigation.

P. Boyer, P. Champeil, F. Guillain, 1984, Biochemistry.

Stopped flow and rapid quenching measurement of the transient steps induced by calcium binding to sarcoplasmic reticulum adenosine triphosphatase. Competition with Ca2+-independent phosphorylation.

P. Champeil, F. Guillain, J. Lacapere, 1981, The Journal of biological chemistry.

Sarcoplasmic reticulum ATPase phosphorylation from inorganic phosphate in the absence of a calcium gradient. Steady state and kinetic fluorescence studies.

P. Champeil, F. Guillain, J. Lacapere, 1981, The Journal of biological chemistry.

A direct fluorescence study of the transient steps induced by calcium binding to sarcoplasmic reticulum ATPase.

P. Champeil, F. Guillain, M. Gingold, 1980, The Journal of biological chemistry.

Calcium transport by sarcoplasmic reticulum Ca(2+)-ATPase. Role of the A domain and its C-terminal link with the transmembrane region.

C. Toyoshima, M. le Maire, P. Champeil, 2002, The Journal of biological chemistry.

Do Transmembrane Segments in Proteolyzed Sarcoplasmic Reticulum Ca2+-ATPase Retain Their Functional Ca2+ Binding Properties after Removal of Cytoplasmic Fragments by Proteinase K? (*)

L. Denoroy, P. Champeil, M. Maire, 1995, The Journal of Biological Chemistry.

Crosslinking the active site of sarcoplasmic reticulum Ca(2+)-ATPase completely blocks Ca2+ release to the vesicle lumen.

P. Champeil, F. Guillain, D. C. Ross, 1991, Proceedings of the National Academy of Sciences of the United States of America.

Detergent structure and associated lipid as determinants in the stabilization of solubilized Ca2+-ATPase from sarcoplasmic reticulum.

S. Orlowski, J. Møller, M. le Maire, 1989, The Journal of biological chemistry.

High phosphatidylinositol 4-phosphate (PI4P)-dependent ATPase activity for the Drs2p-Cdc50p flippase after removal of its N- and C-terminal extensions

J. Lyons, P. Nissen, P. Champeil, 2017, The Journal of Biological Chemistry.

Twists and turns

P. Champeil, M. Maire, JESPER V. MØLLER, 2018, Nature.

Use of metallochromic dyes and potentiometric pH-meter titration to detect binding of divalent cations to "Good's" buffers: 4-morpholinepropanesulfonic acid (Mops) does not bind Mg2+.

P. Champeil, C. Montigny, 2007, Analytical biochemistry.

How to evaluate the distribution of an "invisible" amphiphile between biological membranes and water.

M. le Maire, P. Champeil, B. de Foresta, 1990, Analytical biochemistry.

Stabilization of membranes upon interaction of amphipathic polymers with membrane proteins

M. Picard, P. Champeil, E. Dé, 2004, Protein science : a publication of the Protein Society.

Functional Properties of Sarcoplasmic Reticulum Ca2+-ATPase after Proteolytic Cleavage at Leu119-Lys120, Close to the A-domain

M. Picard, Carole Gauron, J. Møller, 2004, Journal of Biological Chemistry.

Rapid filtration analysis of nucleotide binding to Na,K-ATPase.

P. Champeil, N. Fedosova, M. Esmann, 2003, Biochemistry.

A High-Yield Co-Expression System for the Purification of an Intact Drs2p-Cdc50p Lipid Flippase Complex, Critically Dependent on and Stabilized by Phosphatidylinositol-4-Phosphate

P. Nissen, M. Palmgren, P. Gourdon, 2014, PloS one.

Role of Mg2+ ions in several steps of the sarcoplasmic reticulum-ATPase cycle.

P. Champeil, F. Guillain, J. Lacapere, 1984, Current topics in cellular regulation.

Effects of Inhibitors on Luminal Opening of Ca2+ Binding Sites in an E2P-like Complex of Sarcoplasmic Reticulum Ca22+-ATPase with Be22+-fluoride*

M. Picard, C. Toyoshima, P. Champeil, 2006, Journal of Biological Chemistry.

Calcium Transport by Sarcoplasmic Reticulum Ca2+-ATPase

C. Toyoshima, J. Møller, M. le Maire, 2002, The Journal of Biological Chemistry.

From calcium blips to calcium puffs: theoretical analysis of the requirements for interchannel communication.

S. Swillens, G. Dupont, L. Combettes, 1999, Proceedings of the National Academy of Sciences of the United States of America.

The Topology of Sarcoplasmic Reticulum Ca2+-ATPase and Na+,K+-ATPase in the M5/M6 Region

J. Møller, P. Champeil, M. Maire, 1994 .

Spin labelled sarcoplasmic reticulum vesicles: Ca2+‐induced spectral changes

C. Taupin, P. Champeil, F. Bastide, 1976, FEBS letters.

Inhibition by 4-hydroxynonenal (HNE) of Ca2+ transport by SERCA1a: low concentrations of HNE open protein-mediated leaks in the membrane.

P. Champeil, Solenne Chardonnet, C. Montigny, 2011, Free radical biology & medicine.