I. Urbatsch

发表

Structure of P-Glycoprotein Reveals a Molecular Basis for Poly-Specific Drug Binding

Yue Weng, Geoffrey Chang, G. Chang, 2009, Science.

Covalent inhibitors of P-glycoprotein ATPase activity.

A. E. Senior, I. Urbatsch, M. Al-Shawi, 1994, The Journal of biological chemistry.

Structural stability of purified human CFTR is systematically improved by mutations in nucleotide binding domain 1.

J. Riordan, H. Ding, J. Kappes, 2018, Biochimica et biophysica acta. Biomembranes.

Substitution of Yor1p NBD1 residues improves the thermal stability of Human Cystic Fibrosis Transmembrane Conductance Regulator

H. Ding, J. Kappes, I. Urbatsch, 2017, Protein engineering, design & selection : PEDS.

Distinct conformational spectrum of homologous multidrug ABC transporters.

B. Carragher, C. Potter, G. Chang, 2015, Structure.

Mutational analysis of conserved carboxylate residues in the nucleotide binding sites of P-glycoprotein.

Marc-Etienne Rousseau, P. Gros, I. Urbatsch, 2000, Biochemistry.

Combined Mutation of Catalytic Glutamate Residues in the Two Nucleotide Binding Domains of P-glycoprotein Generates a Conformation That Binds ATP and ADP Tightly*

A. E. Senior, I. Urbatsch, G. Tombline, 2004, Journal of Biological Chemistry.

Drosophila INDY and Mammalian INDY: Major Differences in Transport Mechanism and Structural Features despite Mostly Similar Biological Functions

V. Ganapathy, I. Urbatsch, Sathish Sivaprakasam, 2021, Metabolites.

Bradford Assay for Determining Protein Concentration.

I. Urbatsch, C. Kielkopf, William J. Bauer, 2020, Cold Spring Harbor protocols.

Functional analysis of a tryptophan-less P-glycoprotein: a tool for tryptophan insertion and fluorescence spectroscopy.

T. Kwan, P. Gros, A. E. Senior, 2000, Molecular pharmacology.

Conserved Walker A Ser Residues in the Catalytic Sites of P-glycoprotein Are Critical for Catalysis and Involved Primarily at the Transition State Step*

A. E. Senior, S. Wilke-Mounts, I. Urbatsch, 2000, The Journal of Biological Chemistry.

Purification and ATP hydrolysis of the putative cholesterol transporters ABCG5 and ABCG8.

J. Weber, I. Urbatsch, M. Dumont, 2006, Biochemistry.

Effects of lipids on ATPase activity of purified Chinese hamster P-glycoprotein.

A. E. Senior, I. Urbatsch, 1995, Archives of biochemistry and biophysics.

Expression, purification, and characterization of cysteine-free mouse P-glycoprotein.

A. E. Senior, I. Urbatsch, G. Tombline, 2006, Archives of biochemistry and biophysics.

Mutational analysis of conserved aromatic residues in the A‐loop of the ABC transporter ABCB1A (mouse Mdr3)

P. Gros, A. E. Senior, I. Urbatsch, 2007, FEBS letters.

Methods for Measuring the Concentrations of Proteins.

I. Urbatsch, C. Kielkopf, William J. Bauer, 2020, Cold Spring Harbor protocols.

Investigation of the role of glutamine-471 and glutamine-1114 in the two catalytic sites of P-glycoprotein.

A. E. Senior, S. Wilke-Mounts, I. Urbatsch, 2000, Biochemistry.

Nucleotide dependence of the dimerization of ATP binding cassette nucleotide binding domains.

R B Sutton, I. Urbatsch, G. Altenberg, 2016, Biochemical and biophysical research communications.

Nucleotide binding to the multidrug resistance P-glycoprotein as studied by ESR spectroscopy.

A. E. Senior, I. Urbatsch, S. Delannoy, 2005, Biochemistry.

Staining Sodium Dodecyl Sulfate-Polyacrylamide Gels with Silver Salts.

I. Urbatsch, C. Kielkopf, William J. Bauer, 2021, Cold Spring Harbor protocols.

Design and Synthesis of Selenazole‐Containing Peptides for Cocrystallization with P‐Glycoprotein

G. Chang, Yue Weng, R. Zhuo, 2011, Chembiochem : a European journal of chemical biology.

Expression of Cloned Genes Using the Baculovirus Expression System.

I. Urbatsch, C. Kielkopf, William J. Bauer, 2020, Cold Spring Harbor protocols.

A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris

G. Chang, A. Ward, Brigitte Gasser, 2011, PloS one.

Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains

J. Riordan, L. DeLucas, J. Kappes, 2014, Protein science : a publication of the Protein Society.

Expressing Cloned Genes for Protein Production, Purification, and Analysis.

I. Urbatsch, C. Kielkopf, William J. Bauer, 2020, Cold Spring Harbor protocols.

Replacing the eleven native tryptophans by directed evolution produces an active P-glycoprotein with site-specific, non-conservative substitutions

J. Weber, I. Urbatsch, Anukriti Singh, 2020, Scientific Reports.

P-glycoprotein is fully active after multiple tryptophan substitutions.

J. Weber, I. Urbatsch, D. Swartz, 2013, Biochimica et biophysica acta.

Large Scale Purification of Detergent-soluble P-glycoprotein fromPichia pastoris Cells and Characterization of Nucleotide Binding Properties of Wild-type, Walker A, and Walker B Mutant Proteins*

P. Gros, A. E. Senior, I. Urbatsch, 1999, The Journal of Biological Chemistry.

High-level expression of mouse Mdr3 P-glycoprotein in yeast Pichia pastoris and characterization of ATPase activity.

P. Gros, I. Urbatsch, L. Beaudet, 1998, Methods in enzymology.

The catalytic cycle of P‐glycoprotein

A. E. Senior, I. Urbatsch, A E Senior, 1995, FEBS letters.

Both P-glycoprotein Nucleotide-binding Sites Are Catalytically Active (*)

B. Sankaran, A. E. Senior, I. Urbatsch, 1995, The Journal of Biological Chemistry.

P-glycoprotein Is Stably Inhibited by Vanadate-induced Trapping of Nucleotide at a Single Catalytic Site (*)

B. Sankaran, J. Weber, A. E. Senior, 1995, The Journal of Biological Chemistry.

ATP hydrolysis by multidrug-resistance protein from Chinese hamster ovary cells

A. E. Senior, I. Urbatsch, M. Al-Shawi, 1995, Journal of Bioenergetics and Biomembranes.

Characterization of the ATPase activity of purified Chinese hamster P-glycoprotein.

A. E. Senior, I. Urbatsch, M. Al-Shawi, 1994, Biochemistry.

Domain-interface dynamics of CFTR revealed by stabilizing nanobodies

J. Riordan, E. Pardon, J. Steyaert, 2019, Nature Communications.

A survey of detergents for the purification of stable, active human cystic fibrosis transmembrane conductance regulator (CFTR).

L. DeLucas, H. Ding, J. Kappes, 2014, Biochimica et biophysica acta.

Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain

E. Pardon, J. Steyaert, G. Chang, 2013, Proceedings of the National Academy of Sciences.

Purification and characterization of N-glycosylation mutant mouse and human P-glycoproteins expressed in Pichia pastoris cells.

A. E. Senior, S. Wilke-Mounts, I. Urbatsch, 2001, Archives of biochemistry and biophysics.

Crystal structure of the human sterol transporter ABCG5/ABCG8

Jonathan C. Cohen, H. Hobbs, L. Kinch, 2016, Nature.

Snapshots of ligand entry, malleable binding and induced helical movement in P-glycoprotein

Geoffrey Chang, Steven D. Rees, G. Chang, 2015, Acta crystallographica. Section D, Biological crystallography.

Substrate-induced conformational changes in the nucleotide-binding domains of lipid bilayer–associated P-glycoprotein during ATP hydrolysis

I. Urbatsch, G. Altenberg, Leo Mok, 2017, The Journal of Biological Chemistry.

Interactions and cooperativity between P-glycoprotein structural domains determined by thermal unfolding provides insights into its solution structure and function.

I. Urbatsch, C. Brouillette, Zhengrong Yang, 2017, Biochimica et biophysica acta. Biomembranes.

Nucleotide-induced Structural Changes in P-glycoprotein Observed by Electron Microscopy*

Stephan Wilkens, A. E. Senior, I. Urbatsch, 2008, Journal of Biological Chemistry.

Understanding polyspecificity of multidrug ABC transporters: closing in on the gaps in ABCB1.

Geoffrey Chang, G. Chang, A. Ward, 2010, Trends in biochemical sciences.

Properties of P-glycoprotein with Mutations in the “Catalytic Carboxylate” Glutamate Residues*

A. E. Senior, I. Urbatsch, G. Tombline, 2004, Journal of Biological Chemistry.

Purification of Polyhistidine-Tagged Proteins by Immobilized Metal Affinity Chromatography.

I. Urbatsch, C. Kielkopf, William J. Bauer, 2020, Cold Spring Harbor protocols.

Specific Lipids Modulate the Transporter Associated with Antigen Processing (TAP)*

Christer S. Ejsing, R. Tampé, H. Robenek, 2011, The Journal of Biological Chemistry.

P-glycoprotein Catalytic Mechanism

A. E. Senior, I. Urbatsch, G. Tombline, 2003, Journal of Biological Chemistry.

Projection Structure of P-glycoprotein by Electron Microscopy

A. E. Senior, I. Urbatsch, S. Wilkens, 2002, The Journal of Biological Chemistry.

Cysteines 431 and 1074 Are Responsible for Inhibitory Disulfide Cross-linking between the Two Nucleotide-binding Sites in Human P-glycoprotein*

Marc-Etienne Rousseau, P. Gros, A. E. Senior, 2001, The Journal of Biological Chemistry.

Residues in P-glycoprotein catalytic sites that react with the inhibitor 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole.

P. Gros, A. E. Senior, I. Urbatsch, 1998, Archives of biochemistry and biophysics.

ATPase activity of Chinese hamster P-glycoprotein.

A. E. Senior, I. Urbatsch, M. Al-Shawi, 1998, Methods in enzymology.

Nucleotide Binding to the Human Multidrug Resistance Protein 3, MRP3

I. Urbatsch, P. Vogel, Andrea D Hoffman, 2010, The protein journal.

Mutations in the nucleotide-binding sites of P-glycoprotein that affect substrate specificity modulate substrate-induced adenosine triphosphatase activity.

P. Gros, I. Urbatsch, L. Beaudet, 1998, Biochemistry.

Mutations in either nucleotide-binding site of P-glycoprotein (Mdr3) prevent vanadate trapping of nucleotide at both sites.

P. Gros, I. Urbatsch, L. Beaudet, 1998, Biochemistry.

Asymmetry in inward- and outward-affinity constant of transport explain unidirectional lysine flux in Saccharomyces cerevisiae

Katja Luck, Bert Poolman, Katja Luck, 2016, Scientific Reports.

A Stable Human-Cell System Overexpressing Cystic Fibrosis Transmembrane Conductance Regulator Recombinant Protein at the Cell Surface

J. Riordan, L. DeLucas, H. Ding, 2015, Molecular Biotechnology.

Functional Distinction between Human and Mouse Sodium-Coupled Citrate Transporters and Its Biologic Significance: An Attempt for Structural Basis Using a Homology Modeling Approach.

V. Ganapathy, I. Urbatsch, Valeria Jaramillo-Martinez, 2020, Chemical reviews.

A home run for human NaCT/SLC13A5/INDY: cryo-EM structure and homology model to predict transport mechanisms, inhibitor interactions and mutational defects

V. Ganapathy, I. Urbatsch, Valeria Jaramillo-Martinez, 2021, The Biochemical journal.

Bile acids stimulate ATP hydrolysis in the purified cholesterol transporter ABCG5/G8.

Brandy J. Johnson, I. Urbatsch, A. Pickert, 2010, Biochemistry.

Expression of 25 human ABC transporters in the yeast Pichia pastoris and characterization of the purified ABCC3 ATPase activity.

Yenphuong T. Trinh, I. Urbatsch, M. Dumont, 2007, Biochemistry.

Functional reconstitution of human ABCC3 into proteoliposomes reveals a transport mechanism with positive cooperativity.

H. Galla, I. Urbatsch, Britta Zehnpfennig, 2009, Biochemistry.

Lipid environment determines the drug-stimulated ATPase activity of P-glycoprotein

J. Weber, Qinghai Zhang, I. Urbatsch, 2023, Frontiers in Molecular Biosciences.

Directed evolution of P-glycoprotein cysteines reveals site-specific, non-conservative substitutions that preserve multidrug resistance

I. Urbatsch, G. Altenberg, Leo Mok, 2014, Bioscience reports.

Specific stabilization of CFTR by phosphatidylserine.

J. Kappes, H. Senderowitz, N. Khazanov, 2017, Biochimica et biophysica acta. Biomembranes.

human NBD1 of CFTR in complex with nanobodies T2a and T4

J. Riordan, E. Pardon, J. Steyaert, 2019 .

Mechanism of dual pharmacological correction and potentiation of human CFTR

J. Kappes, R. Grassucci, G. Lukács, 2022, bioRxiv.

Tracing the substrate translocation mechanism in P-glycoprotein

G. Hummer, D. Januliene, A. Moeller, 2023, bioRxiv.

Domain-interface dynamics of CFTR revealed by stabilizing nanobodies

J. Riordan, E. Pardon, J. Steyaert, 2019, Nature Communications.

Staphylococcal food poisoning.

A. L. Wong, J. Gécz, M. Rietschel, 2002 .

Functional analysis of a species-specific inhibitor selective for human Na+-coupled citrate transporter (NaCT/SLC13A5/mINDY)

V. Ganapathy, R B Sutton, I. Urbatsch, 2020, The Biochemical journal.

Variations of Staining Sodium Dodecyl Sulfate-Polyacrylamide Gels with Coomassie Brilliant Blue.

I. Urbatsch, C. Kielkopf, William J. Bauer, 2021, Cold Spring Harbor protocols.

Replacing the eleven native tryptophans by directed evolution produces an active P-glycoprotein with site-specific, non-conservative substitutions

J. Weber, I. Urbatsch, Anukriti Singh, 2020, Scientific Reports.

Scarring Alopecia

S. Girirajan, I. Zusman, H. Meinck, 2020 .

Sitosterolemia

A. L. Wong, J. Gécz, A. Pitkänen, 2020, Definitions.

Sialic Acid Storage Disease

M. Rietschel, M. Vinceti, A. Pitkänen, 2020, Definitions.