J. Sayers

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Invasive isolates of Neisseria meningitidis possess enhanced immunoglobulin A1 protease activity compared to colonizing strains

R. Read, S. Vitovski, J. Sayers, 1999, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

The evolution of pathways for aromatic hydrocarbon oxidation inPseudomonas

P. Williams, J. Sayers, 1994, Biodegradation.

Mutagenesis of conserved lysine residues in bacteriophage T5 5'-3' exonuclease suggests separate mechanisms of endo-and exonucleolytic cleavage.

T. Ceska, D. Suck, S. Garforth, 1999, Proceedings of the National Academy of Sciences of the United States of America.

Viral polymerase-associated 5'-->3'-exonucleases: expression, purification, and uses.

J. Sayers, 1996, Methods in enzymology.

Active site substitutions delineate distinct classes of eubacterial flap endonuclease

J. Sayers, M. Hodskinson, L. M. Allen, 2008, The Biochemical journal.

Identification and eradication of a denatured DNA isolated during alkaline lysis-based plasmid purification procedures.

J. Sayers, J. Thomson, D. Evans, 1996, Analytical biochemistry.

The structure of Escherichia coli ExoIX—implications for DNA binding and catalysis in flap endonucleases

T. J. Stillman, P. Artymiuk, S. Sedelnikova, 2013, Nucleic acids research.

Schistosoma mansoni secretes a chemokine binding protein with antiinflammatory activity

A. Alcamí, Philip Smith, Niamh E Mangan, 2005, The Journal of experimental medicine.

Phosphorothioate-based oligonucleotide-directed mutagenesis

J. Sayers, F. Eckstein, 1988 .

Investigating the interaction between osteoprotegerin and receptor activator of NF-kappaB or tumor necrosis factor-related apoptosis-inducing ligand: evidence for a pivotal role for osteoprotegerin in regulating two distinct pathways.

P. Croucher, S. Vitovski, J. Sayers, 2007, The Journal of biological chemistry.

Immunogenicity, toxicology, pharmacokinetics and pharmacodynamics of growth hormone ligand-receptor fusions.

P. Artymiuk, C. Strasburger, Zida Wu, 2010, Clinical science.

Overview: High Efficiency Oligonucleotide-Directed Mutagenesis of Double-stranded DNA Vectors

J. Sayers, D. Olsen, 1992 .

A method for enhancing the transfection efficiency of minipreps obtained from plasmid cDNA libraries.

E. Kiss-Toth, J. Sayers, S. Dower, 2001, Analytical biochemistry.

Expression of a glycosylphosphatidylinositol-anchored ligand, growth hormone, blocks receptor signalling

P. Artymiuk, D. James, F. Guesdon, 2012, Bioscience reports.

Low-dose methotrexate in myeloproliferative neoplasm models

J. Snowden, J. Sayers, M. Zeidler, 2017, Haematologica.

Structure-specific DNA cleavage by 5' nucleases.

T. Ceska, J. Sayers, 1998, Trends in biochemical sciences.

Three Metal Ions Participate in the Reaction Catalyzed by T5 Flap Endonuclease*

J. Tainer, J. Grasby, Nicholas H Williams, 2008, Journal of Biological Chemistry.

Sunny Chicago and gloomy Oxford: their roles in a citation classic

J. Sayers, 2007 .

Cloning, heterologous expression and antigenicity of a schistosome cercarial protease

J. Sayers, M. Doenhoff, H. Price, 1997, Parasitology.

A single cleavage assay for T5 5'-->3' exonuclease: determination of the catalytic parameters forwild-type and mutant proteins.

J. Grasby, S. Garforth, J. Sayers, 1999, Nucleic acids research.

Dynamic evidence for metal ion catalysis in the reaction mediated by a flap endonuclease

J. Grasby, J. Sayers, M. R. Tock, 2003, The EMBO journal.

MacrophagesProgrammed Death Ligand 1 on of T Cells via Selective Up-Regulation of Worms Induce Anergy Schistosoma mansoni

Philip Smith, C. Walsh, A. McKenzie, 2004 .

INVESTIGATING THE INTERACTION BETWEEN OSTEOPROTEGERIN AND RANKL OR TRAIL: EVIDENCE FOR A PIVOTAL ROLE FOR OSTEOPROTEGERIN IN REGULATING TWO DISTINCT PATHWAYS

P. Croucher, S. Vitovski, J. Sayers, 2007 .

Schistosoma mansoni cercarial elastase (SmCE): differences in immunogenic properties of native and recombinant forms

J. Sayers, M. Doenhoff, H. Price, 2017, Parasitology.

Antibiotics Limit Adaptation of Drug-Resistant Staphylococcus aureus to Hypoxia

S. Foster, M. Brockhurst, J. Sayers, 2022, Antimicrobial agents and chemotherapy.

Synthesis and antiviral properties of (E)-5-(2-bromovinyl)-2'-deoxycytidine-related compounds.

R. T. Walker, E. De Clercq, J. Sayers, 1988, Journal of medicinal chemistry.

The synthesis and antiviral properties of (E)-5-(2-bromovinyl)-2'-deoxyuridine-related compounds

R. T. Walker, E. Clercq, A. Kumar, 1987 .

Site-directed mutagenesis of single-stranded and double-stranded DNA by phosphorothioate approach.

J. Sayers, F. Eckstein, D. Olsen, 1993, Methods in enzymology.

Inhibition of restriction endonuclease hydrolysis by phosphorothioate-containing DNA.

J. Sayers, F. Eckstein, D. Olsen, 1989, Nucleic acids research.

5'-3' exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis.

J. Sayers, F. Eckstein, W. Schmidt, 1988, Nucleic acids research.

A Model for Transition of 5′-Nuclease Domain of DNA Polymerase I from Inert to Active Modes

P. Xie, J. Sayers, 2011, PloS one.

A high content microscopy-based platform for detecting antibodies to the nucleocapsid, spike and membrane proteins of SARS-CoV-2

D. Bliss, A. Metcalfe, J. Sayers, 2021, medRxiv.

Characterization of an unusual bipolar helicase encoded by bacteriophage T5

C. M. Sanders, J. Sayers, Io Nam Wong, 2013, Nucleic acids research.

Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust.

S. Rowland-Jones, A. Kucharski, D. James, 2022, Wellcome open research.

Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust

S. Rowland-Jones, A. Kucharski, D. James, 2021, Wellcome open research.

Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust

S. Rowland-Jones, A. Kucharski, D. James, 2021, medRxiv.

Title: Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust.

A. Kucharski, D. James, J. Sayers, 2021 .

Schistosoma mansoni: anomalous immunogenic properties of a 27 kDa larval serine protease associated with protective immunity

J. Sayers, R. Curtis, M. Doenhoff, 1997, Parasitology.

AGA/AGG codon usage in parasites: implications for gene expression in Escherichia coli.

P. Fallon, J. Sayers, M. Doenhoff, 1995, Parasitology today.

Relaxed Cleavage Specificity of an Immunoglobulin A1 Protease from Neisseria meningitidis

S. Vitovski, J. Sayers, 2007, Infection and Immunity.

Ligand‐Receptor Fusion Dimers

P. Artymiuk, R. Ross, J. Sayers, 2013 .

A ligand-receptor fusion of growth hormone forms a dimer and is a potent long-acting agonist

P. Artymiuk, C. Strasburger, Zida Wu, 2007, Nature Medicine.

Nontypeable Haemophilus influenzae in carriage and disease: a difference in IgA1 protease activity levels.

S. Vitovski, J. Sayers, A. Howard, 2002, JAMA.

Rapid high-efficiency site-directed mutagenesis by the phosphorothioate approach.

J. Sayers, F. Eckstein, C. Krekel, 1992, BioTechniques.

Schistosoma mansoni Worms Induce Anergy of T Cells via Selective Up-Regulation of Programmed Death Ligand 1 on Macrophages1

Philip Smith, C. Walsh, R. Fallon, 2004, The Journal of Immunology.

Roles of divalent metal ions in flap endonuclease–substrate interactions

Dipak Patel, Dietrich Suck, Min Feng, 2004, Nature Structural &Molecular Biology.

Direct observation of DNA threading in flap endonuclease complexes

T. Ceska, P. Artymiuk, J. Rafferty, 2016, Nature Structural &Molecular Biology.

A conserved tyrosine residue aids ternary complex formation, but not catalysis, in phage T5 flap endonuclease.

J. Grasby, J. Sayers, M. Feng, 2002, Journal of molecular biology.

Interactions of mutant and wild-type flap endonucleases with oligonucleotide substrates suggest an alternative model of DNA binding

T. Ceska, P. Artymiuk, J. Grasby, 2002, Proceedings of the National Academy of Sciences of the United States of America.

A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease

T. Ceska, D. Suck, G. Stier, 1996, Nature.

Continuous affinity-based selection: rapid screening and simultaneous amplification of bacterial surface-display libraries.

S. Vitovski, J. Sayers, R. Hockney, 2001, The Biochemical journal.

Variation in the Steady State Kinetic Parameters of Wild Type and Mutant T5 5′-3′-Exonuclease With pH

S. Garforth, J. Sayers, T. J. Pickering, 1999, The Journal of Biological Chemistry.

Prokaryotic 5'-3' exonucleases share a common core structure with gamma-delta resolvase.

T. Ceska, D. Suck, P. Artymiuk, 1997, Nucleic acids research.

Structure-specific DNA binding by bacteriophage T5 5'-->3' exonuclease.

S. Garforth, J. Sayers, Jon R. Sayers, 1997, Nucleic acids research.

A single-strand specific endonuclease activity copurifies with overexpressed T5 D15 exonuclease.

J. Sayers, F. Eckstein, 1991, Nucleic acids research.

A long-acting GH receptor antagonist through fusion to GH binding protein

P. Artymiuk, R. Ross, J. Sayers, 2016, Scientific Reports.

Properties of overexpressed phage T5 D15 exonuclease. Similarities with Escherichia coli DNA polymerase I 5'-3' exonuclease.

J. Sayers, F. Eckstein, 1990, The Journal of biological chemistry.

Comparison of the catalytic parameters and reaction specificities of a phage and an archaeal flap endonuclease.

J. Tainer, J. Grasby, Karl Syson, 2007, Journal of molecular biology.

Unusually wide co-factor tolerance in a metalloenzyme; divalent metal ions modulate endo?exonuclease activity in T5 exonuclease

Scott J. Garforth, Dipak Patel, Min Feng, 2001, Nucleic Acids Res..

Flexible loops and helical arches

P. Artymiuk, J. Sayers, 1998, Nature Structural &Molecular Biology.

Immunoglobulin A1 proteases: a structure-function update.

S. Vitovski, J. Sayers, H. Parsons, 2004, Biochemical Society transactions.

Bacteriophage T5 gene D10 encodes a branch-migration protein

C. M. Sanders, J. Sayers, Io Nam Wong, 2016, Scientific Reports.

Neutralizing Mutations of Carboxylates That Bind Metal 2 in T5 Flap Endonuclease Result in an Enzyme That Still Requires Two Metal Ions*

J. Tainer, J. Grasby, Nicholas H Williams, 2011, The Journal of Biological Chemistry.

Bacteriophage collagen [5] (multiple letters)

S. Casjens, J. Sayers, R. Hendrix, 1998 .

Bacteriophage Collagen

S. Casjens, J. Sayers, R. Hendrix, 1998, Science.

Phosphorothioate-Based Site-Directed Mutagenesis: Extending the Basic Methodology

J. Sayers, F. Eckstein, W. Schmidt, 1988 .

Strand specific cleavage of phosphorothioate-containing DNA by reaction with restriction endonucleases in the presence of ethidium bromide.

J. Sayers, F. Eckstein, A. Wendler, 1988, Nucleic acids research.

Establishing SARS-CoV-2 membrane protein-specific antibodies as a valuable serological target via high-content microscopy

J. Sayers, T. D. de Silva, Rebecca Brown, 2023, iScience.

Molecular interactions of Escherichia coli ExoIX and identification of its associated 3′–5′ exonuclease activity

J. Sayers, M. Hodskinson, L. M. Allen, 2007, Nucleic acids research.

Computer aided identification of a potential 5'-3' exonuclease gene encoded by Escherichia coli.

J. Sayers, 1994, Journal of theoretical biology.

Preliminary crystallographic studies on the D15 5' to 3' exonuclease from phage T5.

T. Ceska, D. Suck, J. Sayers, 1993, Journal of molecular biology.

Establishing SARS-CoV-2 membrane protein-specific antibodies as a valuable serological target via high-content microscopy

J. Sayers, T. D. de Silva, Rebecca Brown, 2023, iScience.

Brønsted analysis and rate-limiting steps for the T5 flap endonuclease catalyzed hydrolysis of exonucleolytic substrates.

J. Grasby, Nicholas H Williams, J. Sayers, 2010, Biochemistry.

Inhibition of the restriction endonuclease BanII using modified DNA substrates. Determination of phosphate residues critical for the formation of an active enzyme-DNA complex.

J. Sayers, F. Eckstein, D. Olsen, 1990, Journal of Biological Chemistry.

Nontypeable Haemophilus influenzae in Carriage and Disease

S. Vitovski, J. Sayers, A. Howard, 2017 .

Schistosoma mansoni secretes a chemokine binding protein with antiinflammatory activity

A. Alcamí, Philip Smith, C. Walsh, 2005, The Journal of experimental medicine.

Schistosoma mansoni secretes a chemokine binding protein with antiinflammatory activity

A. Alcamí, Philip Smith, C. Walsh, 2005, The Journal of experimental medicine.