D. Roper

发表

Nano-encapsulated Escherichia coli Divisome Anchor ZipA, and in Complex with FtsZ

Benjamin S. Hanson, K. Edler, Mohammed Jamshad, 2019, Scientific Reports.

Bacterial cell wall assembly: still an attractive antibacterial target.

T. Bugg, C. Dowson, D. Roper, 2011, Trends in biotechnology.

Phospho-MurNAc-pentapeptide translocase (MraY) as a target for antibacterial agents and antibacterial proteins.

A. Lloyd, T. Bugg, D. Roper, 2006, Infectious disorders drug targets.

A molecular link between cell wall biosynthesis, translation fidelity, and stringent response in Streptococcus pneumoniae

A. Lloyd, Surya D. Aggarwal, N. Hiller, 2021, Proceedings of the National Academy of Sciences.

Asymmetric triplex metallohelices with high and selective activity against cancer cells.

Rebecca A. Kaner, G. Clarkson, D. Roper, 2014, Nature chemistry.

Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases.

Z. Dauter, D. Wigley, K. Wilson, 1996, Biochemistry.

Structural and mechanistic studies of the orf12 gene product from the clavulanic acid biosynthesis pathway.

A. Dubus, I. Andersson, D. Ivison, 2013, Acta crystallographica. Section D, Biological crystallography.

Observation of the time-course for peptidoglycan lipid intermediate II polymerization by Staphylococcus aureus monofunctional transglycosylase.

M. Chappell, T. Bugg, D. Roper, 2014, Microbiology.

In vitro reconstitution of peptidoglycan assembly from the Gram-positive pathogen Streptococcus pneumoniae.

T. Vernet, E. Breukink, A. Zapun, 2013, ACS chemical biology.

Design and synthesis of new hydroxyethylamines as inhibitors of D-alanyl-D-lactate ligase (VanA) and D-alanyl-D-alanine ligase (DdlB).

Samo Turk, Stanislav Gobec, C. Fishwick, 2009, Bioorganic & medicinal chemistry letters.

Mutational analysis of the substrate specificity of Escherichia coli penicillin binding protein 4.

Thomas B. Clarke, F. Kawai, J. Tame, 2009, Biochemistry.

Tetramerization of ZapA is required for FtsZ bundling.

M. Hicks, A. Rodger, Corinne J. Smith, 2013, The Biochemical journal.

In vitro characterization of the antivirulence target of Gram-positive pathogens, peptidoglycan O-acetyltransferase A (OatA)

P. Howell, H. Robinson, C. Dowson, 2017, PLoS pathogens.

Crystal structures of penicillin-binding protein 3 (PBP3) from methicillin-resistant Staphylococcus aureus in the apo and cefotaxime-bound forms.

E. Obayashi, H. Yoshida, F. Kawai, 2012, Journal of molecular biology.

A road map for prioritizing warheads for cysteine targeting covalent inhibitors.

J. Kos, G. Keserű, J. Ilaš, 2018, European journal of medicinal chemistry.

Bacterial Cell Division: Experimental and Theoretical Approaches to the Divisome

H. A. van den Berg, Claire E Broughton, A. Rodger, 2015, Science progress.

Bacterial Lipid II Analogs: Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities

C. Dowson, D. Roper, A. Bouhss, 2019, Molecules.

Structure-based modelling and dynamics of MurM, a Streptococcus pneumoniae penicillin resistance determinant that functions at the cytoplasmic membrane interface

Charo I. del Genio, S. Khalid, A. Lloyd, 2020, bioRxiv.

Structure-guided enhancement of selectivity of chemical probe inhibitors targeting bacterial seryl-tRNA synthetase

C. Fishwick, L. Czaplewski, D. Scott, 2019, bioRxiv.

The crystal structure and oligomeric form of Escherichia colil,d-carboxypeptidase A.

J. Tame, S. Akashi, D. Roper, 2018, Biochemical and biophysical research communications.

The crystal structure and oligomeric form of Escherichia colil,d-carboxypeptidase A.

J. Tame, S. Akashi, D. Roper, 2018, Biochemical and biophysical research communications.

CO2 directly modulates connexin 26 by formation of carbamate bridges between subunits

N. Dale, T. Rodgers, M. Cann, 2013, eLife.

Structure of dual BON-domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation

I. Henderson, T. Lithgow, A. Cunningham, 2020, eLife.

Structure-function analyses of dual-BON domain protein DolP identifies phospholipid binding as a new mechanism for protein localisation

I. Henderson, M. Overduin, T. Lithgow, 2020 .

The physico-chemical characterization of a boiling stable antifreeze protein from a perennial grass (Lolium perenne).

P. Pudney, P. Lillford, C. Sidebottom, 2003, Archives of biochemistry and biophysics.

The role of the jaw subdomain of peptidoglycan glycosyltransferases for lipid II polymerization

S. Khalid, D. Scott, A. Lloyd, 2018, Cell surface.

Characterization of tRNA-dependent Peptide Bond Formation by MurM in the Synthesis of Streptococcus pneumoniae Peptidoglycan*

A. Tomasz, A. Lloyd, T. Bugg, 2008, Journal of Biological Chemistry.

Penicillin-Binding Protein 4

J. Tame, D. Roper, 2013 .

Plant peptidoglycan precursor biosynthesis: Conservation between moss chloroplasts and Gram-negative bacteria

A. Cuming, A. Lloyd, C. Dowson, 2022, bioRxiv.

Diaryltriazenes as antibacterial agents against methicillin resistant Staphylococcus aureus (MRSA) and Mycobacterium smegmatis.

A. Lloyd, C. Dowson, M. Osmak, 2017, European journal of medicinal chemistry.

Crystallization and preliminary X-ray analysis of a D-alanyl-D-alanine ligase (EcDdlB) from Escherichia coli.

V. Fülöp, D. Roper, D. Rea, 2010, Acta crystallographica. Section F, Structural biology and crystallization communications.

Nod1 Signaling Overcomes Resistance of S. pneumoniae to Opsonophagocytic Killing

Thomas B. Clarke, J. Weiser, E. Lysenko, 2007, PLoS pathogens.

Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein

A. Pitt, C. Spickett, S. Hall, 2019, Scientific Reports.

The Pneumococcal Divisome: Dynamic Control of Streptococcus pneumoniae Cell Division

M. Winkler, D. Roper, K. E. Bruce, 2021, Frontiers in Microbiology.

Crystallization and preliminary X-ray characterization of VanA from Enterococcus faecium BM4147: towards the molecular basis of bacterial resistance to the glycopeptide antibiotic vancomycin.

D. Roper, T. Huyton, 1999, Acta crystallographica. Section D, Biological crystallography.

Preliminary crystallographic analysis of 4-oxalocrotonate tautomerase reveals the oligomeric structure of the enzyme.

D. Wigley, H. Subramanya, V. Shingler, 1994, Journal of molecular biology.

Investigating the effect of bacteriophages on bacterial FtsZ localisation

Antonia P. Sagona, U. Qimron, D. Roper, 2022, Frontiers in Cellular and Infection Microbiology.

Subcloning and nucleotide sequence of the 3,4‐dihydroxyphenylacetate (homoprotocatechuate) 2,3‐dioxygenase gene from Escherichia coli C

D. Roper, R. Cooper, 1990, FEBS letters.

Surfactant-free puri fi cation of membrane protein complexes from bacteria : application to the staphylococcal penicillin-binding protein complex PBP 2 / PBP 2 a

S. Foster, Mohammed Jamshad, P. Taylor, 2014 .

Kinetic Characterization of Lipid II-Ala:Alanyl-tRNA Ligase (MurN) from Streptococcus pneumoniae using Semisynthetic Aminoacyl-lipid II Substrates*

A. Lloyd, T. Bugg, J. Schouten, 2008, Journal of Biological Chemistry.

In silico identification, synthesis and biological evaluation of novel tetrazole inhibitors of MurB

C. Dowson, D. Roper, D. Patin, 2018, Chemical biology & drug design.

Indistinguishability and identifiability of kinetic models for the Mur C reaction in peptidoglycan biosynthesis* *EPSRC funded project Indistinguishability analysis for model discrimination in Systems Biology: A Feasibility Study applied to Bacterial Peptidoglycan Biosynthesis (EP/E057535/1).

Daniel J. Bearup, M. Chappell, N. Evans, 2009 .

Mechanisms of Incorporation for D-Amino Acid Probes That Target Peptidoglycan Biosynthesis

F. Cava, E. Breukink, E. Kuru, 2019, ACS chemical biology.

Purification, nucleotide sequence and some properties of a bifunctional isomerase/decarboxylase from the homoprotocatechuate degradative pathway of Escherichia coli C.

D. Roper, R. Cooper, 1993, European journal of biochemistry.

Inhibition of tRNA-dependent ligase MurM from Streptococcus pneumoniae by phosphonate and sulfonamide inhibitors.

S. Caddick, A. Lloyd, T. Bugg, 2009, Bioorganic & medicinal chemistry.

Structure-based modeling and dynamics of MurM, a Streptococcus pneumoniae penicillin resistance determinant present at the cytoplasmic membrane

Charo I. del Genio, S. Khalid, A. Lloyd, 2021, Structure.

Adenosine phosphonate inhibitors of lipid II: alanyl tRNA ligase MurM from Streptococcus pneumoniae.

A. Lloyd, T. Bugg, C. Dowson, 2007, Bioorganic & medicinal chemistry letters.

Crystal structure of penicillin binding protein 4 (dacB) from Escherichia coli, both in the native form and covalently linked to various antibiotics.

J. Tame, Sam-Yong Park, A. Lloyd, 2006, Biochemistry.

Vancomycin resistance in enterococci: reprogramming of the D-ala-D-Ala ligases in bacterial peptidoglycan biosynthesis.

C. Walsh, D. Roper, J. Knox, 2000, Chemistry & biology.

Identification of a Novel Inhibition Site in Translocase MraY Based upon the Site of Interaction with Lysis Protein E from Bacteriophage ϕX174

R. Hancock, T. Bugg, R. Hancock, 2014, Chembiochem : a European journal of chemical biology.

Synthesis and in vitro enzyme activity of an oxa analogue of azi-DAP

R. Cox, D. Roper, J. Durston, 2002 .

Structure-guided enhancement of selectivity of chemical probe inhibitors targeting bacterial seryl-tRNA synthetase.

C. Fishwick, L. Czaplewski, D. Scott, 2019, Journal of medicinal chemistry.

Preliminary crystallographic analysis of 5-carboxymethyl-2-hydroxymuconate isomerase from Escherichia coli.

D. Wigley, D. Roper, R. Cooper, 1989, Journal of molecular biology.

Understanding the mechanism of ice binding by type III antifreeze proteins.

P. Lillford, A. Antson, R. Hubbard, 2001, Journal of molecular biology.

Surfactant-free purification of membrane protein complexes from bacteria: application to the staphylococcal penicillin-binding protein complex PBP2/PBP2a

S. Foster, Mohammed Jamshad, P. Taylor, 2014, Nanotechnology.

Carbohydrate scaffolds as glycosyltransferase inhibitors with in vivo antibacterial activity

W. Meutermans, A. Lloyd, T. Cheng, 2015, Nature Communications.

Fluorescent reagents for in vitro studies of lipid-linked steps of bacterial peptidoglycan biosynthesis: derivatives of UDPMurNAc-pentapeptide containing d-cysteine at position 4 or 5.

A. Lloyd, T. Bugg, J. Schouten, 2006, Molecular bioSystems.

Prospects for novel inhibitors of peptidoglycan transglycosylases

D. Roper, Nicola F. Galley, Amy O’Reilly, 2014, Bioorganic chemistry.

Active membrane transport and receptor proteins from bacteria.

V. Blessie, P. Henderson, M. Phillips-Jones, 2005, Biochemical Society transactions.

Structure/function studies on a S-adenosyl-L-methionine-dependent uroporphyrinogen III C methyltransferase (SUMT), a key regulatory enzyme of tetrapyrrole biosynthesis.

E. Getzoff, K. Wilson, D. Roper, 2004, Journal of molecular biology.

Expression, purification and crystallization of 2-oxo-hept-4-ene-1,7-dioate hydratase (HpcG) from Escherichia coli C

J. Tame, Sam-Yong Park, A. Izumi, 2006, Acta crystallographica. Section F, Structural biology and crystallization communications.

The pH Dependence of Polymerization and Bundling by the Essential Bacterial Cytoskeltal Protein FtsZ

A. Rodger, D. Roper, T. Dafforn, 2011, PloS one.

, Vilmos of a MurE Enzyme Ternary Complex StructurePeptidoglycan as Revealed by the Staphylococcus aureus Incorporation in Specificity Determinants for Lysine Microbiology

V. Fülöp, A. Lloyd, D. Mengin-Lecreulx, 2013 .

Crystal structure and functional assignment of YfaU, a metal ion dependent class II aldolase from Escherichia coli K12.

John A Gerlt, V. Fülöp, J. Gerlt, 2008, Biochemistry.

A conserved zinc-binding site in Acinetobacter baumannii PBP2 required for elongasome-directed bacterial cell shape

Yunfei Dai, A. Lloyd, R. Isberg, 2023, Proceedings of the National Academy of Sciences of the United States of America.

The Extracellular Domain of Two-component System Sensor Kinase VanS from Streptomyces coelicolor Binds Vancomycin at a Newly Identified Binding Site

Richard J. Edwards, D. Roper, C. Lockey, 2020, Scientific Reports.

Structural characterisation of the insecticidal toxin XptA1, reveals a 1.15 MDa tetramer with a cage-like structure.

V. Fülöp, L. Baxter, S. Stoilova-McPhie, 2007, Journal of molecular biology.

FtsZ polymer-bundling by the Escherichia coli ZapA orthologue, YgfE, involves a conformational change in bound GTP.

D. Scott, A. Rodger, D. Roper, 2007, Journal of molecular biology.

The N-terminal domain of human topoisomerase IIalpha is a DNA-dependent ATPase.

A. Maxwell, D. Roper, T. Hammonds, 1998, Biochemistry.

Mutations in the DNA Mismatch Repair Proteins MutS and MutL of Oxazolidinone-Resistant or -Susceptible Enterococcus faecium

S. North, N. Woodford, R. Willems, 2003, Antimicrobial Agents and Chemotherapy.

The mechanics of FtsZ fibers.

M. S. Turner, A. Rodger, Corinne J. Smith, 2012, Biophysical journal.

Purification, some properties and nucleotide sequence of 5‐carboxymethyl‐2‐hydroxymuconate isomerase of Escherichia coli C

D. Roper, R. Cooper, 1990, FEBS letters.

Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein

A. Pitt, C. Spickett, Sarah C. Lee, 2019, Scientific Reports.

Expression, purification and preliminary crystallographic analysis of 2,4-dihydroxy-hepta-2-ene-1,7-dioate aldolase (HpcH) from Escherichia coli C.

V. Fülöp, T. Bugg, D. Roper, 2005, Acta crystallographica. Section F, Structural biology and crystallization communications.

Expression, purification and crystallization of the cell-division protein YgfE from Escherichia coli.

D. Scott, A. Rodger, Corinne J. Smith, 2005, Acta crystallographica. Section F, Structural biology and crystallization communications.

Expression, purification, crystallization and preliminary characterization of uridine 5'-diphospho-N-acetylmuramoyl L-alanyl-D-glutamate:lysine ligase (MurE) from Streptococcus pneumoniae 110K/70.

V. Fülöp, A. Lloyd, T. Bugg, 2004, Acta crystallographica. Section D, Biological crystallography.

Biological Insights from a Simulation Model of the Critical FtsZ Accumulation Required for Prokaryotic Cell Division.

H. A. van den Berg, A. Rodger, D. Roper, 2015, Biochemistry.

A model of membrane contraction predicting initiation and completion of bacterial cell division.

H. A. van den Berg, A. Rodger, D. Roper, 2013, Integrative biology : quantitative biosciences from nano to macro.

Human DNA‐topoisomerase I activity is affected by bis‐netropsin's binding to DNA minor groove

A. Sukhanova, D. Roper, S. Grokhovsky, 1998 .

Inhibition of D-Ala:D-Ala ligase through a phosphorylated form of the antibiotic D-cycloserine

V. Fülöp, C. Fishwick, K. Simmons, 2017, Nature Communications.

Crystallization and preliminary X-ray analysis of a UDP-MurNAc-tripeptide D-alanyl-D-alanine-adding enzyme (PaMurF) from Pseudomonas aeruginosa.

D. Roper, S. Gobec, V. Majce, 2013, Acta crystallographica. Section F, Structural biology and crystallization communications.

Synthetic Sansanmycin Analogues as Potent Mycobacterium tuberculosis Translocase I Inhibitors.

Roger G. Linington, E. Rubin, R. Payne, 2021, Journal of medicinal chemistry.

Sansanmycin natural product analogues as potent and selective anti-mycobacterials that inhibit lipid I biosynthesis

Roger G. Linington, R. Payne, T. Bugg, 2017, Nature Communications.

Substitutions in PBP2b from β-Lactam-resistant Streptococcus pneumoniae Have Different Effects on Enzymatic Activity and Drug Reactivity*

E. Breukink, A. Zapun, C. Contreras-Martel, 2017, The Journal of Biological Chemistry.

Insights into bacterial cell division from a structure of EnvC bound to the FtsX periplasmic domain

P. Stansfeld, D. Roper, J. Cook, 2020, Proceedings of the National Academy of Sciences.

SMA-PAGE: A new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis.

G. Hurlbut, Saskia E. Bakker, S. Muench, 2019, Biochimica et biophysica acta. Biomembranes.

Structure and mechanism of HpcG, a hydratase in the homoprotocatechuate degradation pathway of Escherichia coli.

J. Tame, Sam-Yong Park, S. Unzai, 2007, Journal of molecular biology.

Indistinguishability and identifiability of kinetic models for the MurC reaction in peptidoglycan biosynthesis

Christopher G. Dowson, Neil D. Evans, Michael J. Chappell, 2011, Comput. Methods Programs Biomed..

Pseudomonas aeruginosa MurE amide ligase: enzyme kinetics and peptide inhibitor.

A. Lloyd, T. Bugg, F. Sanschagrin, 2009, The Biochemical journal.

The crystal structure of HpcE, a bifunctional decarboxylase/isomerase with a multifunctional fold.

K. Namba, J. Tame, E. Dodson, 2002, Biochemistry.

Structure and mechanism of HpcH: a metal ion dependent class II aldolase from the homoprotocatechuate degradation pathway of Escherichia coli.

V. Fülöp, T. Bugg, D. Roper, 2007, Journal of molecular biology.

Specificity Determinants for Lysine Incorporation in Staphylococcus aureus Peptidoglycan as Revealed by the Structure of a MurE Enzyme Ternary Complex*

V. Fülöp, A. Lloyd, D. Mengin-Lecreulx, 2013, The Journal of Biological Chemistry.

Circular dichroism spectroscopy for the study of protein-ligand interactions.

A. Rodger, D. Roper, R. Marrington, 2005, Methods in molecular biology.

Adenosine tetraphosphoadenosine drives a continuous ATP-release assay for aminoacyl-tRNA synthetases and other adenylate-forming enzymes.

C. Fishwick, A. Lloyd, C. Dowson, 2013, ACS chemical biology.

Core Steps of Membrane-Bound Peptidoglycan Biosynthesis: Recent Advances, Insight and Opportunities

D. Roper, A. Teo, 2015, Antibiotics.

The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA).

A. Vagin, D. Roper, G. Dodson, 2000, Proceedings of the National Academy of Sciences of the United States of America.

Anticancer metallohelices: nanomolar potency and high selectivity† †Electronic supplementary information (ESI) available: Experimental details, syntheses, biophysical analyses, antimicrobial, anticancer, toxicity, and mechanistic studies. See DOI: 10.1039/c5sc03677a Click here for additional data fi

Rebecca A. Kaner, D. Roper, N. Waterfield, 2015, Chemical science.

Structural basis of lipopolysaccharide maturation by the O-antigen ligase

S. Erramilli, K. Nosol, A. Kossiakoff, 2022, Nature.

Crystal structures of penicillin-binding proteins 4 and 5 from Haemophilus influenzae.

Thomas B. Clarke, E. Obayashi, F. Kawai, 2010, Journal of molecular biology.

A Dynamic Network of Proteins Facilitate Cell Envelope Biogenesis in Gram-Negative Bacteria

D. Roper, Katie Smart, M. Banzhaf, 2021, International journal of molecular sciences.

Beyond the Discovery Void: New Targets for Antibacterial Compounds

H. A. van den Berg, Claire E Broughton, A. Rodger, 2016, Science progress.

Full-length structure of the major autolysin LytA.

T. Vernet, Cong-Zhao Zhou, C. Morlot, 2015, Acta crystallographica. Section D, Biological crystallography.

A de novo designed inhibitor of D-Ala-D-Ala ligase from E. coli.

C. Fishwick, A. Johnson, I. Chopra, 2005, Angewandte Chemie.

Inhibition of D-Ala:D-Ala ligase through a phosphorylated form of the antibiotic D-cycloserine

V. Fülöp, C. Fishwick, K. Simmons, 2017, Nature Communications.

Activator-induced conformational changes regulate division-associated peptidoglycan amidases

P. Stansfeld, D. Roper, J. Cook, 2023, Proceedings of the National Academy of Sciences of the United States of America.

Substrate and stereochemical control of peptidoglycan crosslinking by transpeptidation by Escherichia coli PBP1B.

M. Chappell, N. Evans, A. Lloyd, 2020, Journal of the American Chemical Society.

Rebecca A. Kaner, D. Roper, N. Waterfield, 2015, Chemical Science.

The Escherichia coli C homoprotocatechuate degradative operon: hpc gene order, direction of transcription and control of expression

D. Roper, T. Fawcett, R. Cooper, 1993, Molecular and General Genetics MGG.

Reconstruction of diaminopimelic acid biosynthesis allows characterisation of Mycobacterium tuberculosis N-succinyl-L,L-diaminopimelic acid desuccinylase

G. Besra, G. Kozlov, K. Gehring, 2016, Scientific Reports.

The Extracellular Domain of Two-component System Sensor Kinase VanS from Streptomyces coelicolor Binds Vancomycin at a Newly Identified Binding Site

Richard J. Edwards, D. Roper, A. M. Dixon, 2020, Scientific Reports.

Structure of the diaminopimelate epimerase DapF from Mycobacterium tuberculosis.

G. Besra, K. Fütterer, D. Roper, 2009, Acta crystallographica. Section D, Biological crystallography.

Refinement of Haemophilus influenzae diaminopimelic acid epimerase (DapF) at 1.75 A resolution suggests a mechanism for stereocontrol during catalysis.

J. Turkenburg, A. Lloyd, D. Roper, 2004, Acta crystallographica. Section D, Biological crystallography.

Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex

John J. Deering, F. Mancia, O. Clarke, 2023, Nature communications.

Structure of the diaminopimelate epimerase DapF from Mycobacterium tuberculosis.

G. Besra, K. Fütterer, D. Roper, 2009, Acta crystallographica. Section D, Biological crystallography.