K. Geoghegan

发表

Spontaneous alpha-N-6-phosphogluconoylation of a "His tag" in Escherichia coli: the cause of extra mass of 258 or 178 Da in fusion proteins.

P. Lemotte, K. Geoghegan, H. Dixon, 1999, Analytical biochemistry.

Identification, Characterization, and Crystal Structure of the Omega Class Glutathione Transferases*

G Chelvanayagam, G. Chelvanayagam, D. Danley, 2000, The Journal of Biological Chemistry.

Binding site elucidation and structure guided design of macrocyclic IL-17A antagonists

Li Xing, Guoyun Bai, Atli Thorarensen, 2016, Scientific Reports.

Kinetic analysis of estrogen receptor/ligand interactions

Rebecca L Rich, David G Myszka, Preston Hensley, 2002, Proceedings of the National Academy of Sciences of the United States of America.

X-ray analyses of peptide–inhibitor complexes define the structural basis of specificity for human and mouse renins

T. L. Blundell, V. Dhanaraj, H. P. C. Driessen, 1992, Nature.

Chemoproteomic Analysis of Intertissue and Interspecies Isoform Diversity of AMP-activated Protein Kinase (AMPK)

K. Geoghegan, Jiang Wu, Xidong Feng, 2013, The Journal of Biological Chemistry.

The 2.0 Å crystal structure of the ERα ligand‐binding domain complexed with lasofoxifene

M. Ammirati, P. Lemotte, D. Danley, 2007, Protein Science.

Chemical Modification of Proteins: An Overview

K. Geoghegan, R. Feeney, R. Yamasaki, 1982 .

Structural basis for AMPK activation: natural and synthetic ligands regulate kinase activity from opposite poles by different molecular mechanisms.

R. Kurumbail, M. Harris, Hong Wang, 2014, Structure.

Escherichia coli expression, purification and characterization of functional full-length recombinant alpha2beta2gamma3 heterotrimeric complex of human AMP-activated protein kinase.

R. Kurumbail, M. Harris, K. Geoghegan, 2010, Protein expression and purification.

Cleavage Efficiency by Adenovirus Protease Is Site-dependent*

K. Geoghegan, M. Diouri, J. Weber, 1996, The Journal of Biological Chemistry.

MAP4K4 Is a Threonine Kinase That Phosphorylates FARP1.

L. Buckbinder, K. Stone, Adam G Schwaid, 2015, ACS chemical biology.

Expression and protein chemistry yielding crystallization of the catalytic domain of ADAM17 complexed with a hydroxamate inhibitor.

I. Wang, K. Geoghegan, L. R. Hoth, 2007, Protein expression and purification.

Dominance of Amyloid Precursor Protein Sequence over Host Cell Secretases in Determining β-Amyloid Profiles Studies of Interspecies Variation and Drug Action by Internally Standardized Immunoprecipitation/Mass Spectrometry

K. Geoghegan, M. Rosner, Michele H. Rosner, 2007, Journal of Pharmacology and Experimental Therapeutics.

Reversible reductive alkylation of amino groups in proteins.

K. Geoghegan, R. Feeney, D. Ybarra, 1979, Biochemistry.

Emerging Methods in Chemoproteomics with Relevance to Drug Discovery.

Graham M. West, K. Geoghegan, G. M. West, 2017, Methods in molecular biology.

Direct photocapture of bromodomains using tropolone chemical probes

R. Denny, M. Pletcher, E. Hett, 2015 .

Pharmacologic Inhibition of Site 1 Protease Activity Inhibits Sterol Regulatory Element-Binding Protein Processing and Reduces Lipogenic Enzyme Gene Expression and Lipid Synthesis in Cultured Cells and Experimental Animals

I. Wang, B. Hay, K. Geoghegan, 2008, Journal of Pharmacology and Experimental Therapeutics.

A tag-free collisionally induced fragmentation approach to detect drug-adducted proteins by mass spectrometry.

K. Geoghegan, A. Vaz, T. E. Ballard, 2015, Rapid communications in mass spectrometry : RCM.

Rational targeting of active-site tyrosine residues using sulfonyl fluoride probes.

A. Narayanan, A. Gopalsamy, E. Hett, 2015, ACS chemical biology.

Discovery of a JAK3-Selective Inhibitor: Functional Differentiation of JAK3-Selective Inhibition over pan-JAK or JAK1-Selective Inhibition.

J. Bauman, J. D. Clark, M. Hegen, 2016, ACS chemical biology.

A library approach to rapidly discover photoaffinity probes of the mRNA decapping scavenger enzyme DcpS.

A. Narayanan, A. Gopalsamy, E. Hett, 2015, Molecular bioSystems.

Site‐Directed Conjugation of Nonpeptide Groups to Peptides and Proteins via Periodate Oxidation of a 2‐Amino Alcohol. Application to Modification at N‐Terminal Serine.

K. Geoghegan, J. Stroh, 1992 .

Development of a high-throughput crystal structure-determination platform for JAK1 using a novel metal-chelator soaking system.

R. Kurumbail, K. Geoghegan, L. R. Hoth, 2016, Acta crystallographica. Section F, Structural biology communications.

In vivo dipeptidylation of the amino terminus of recombinant glucagon-like peptide-1(7-37) produced in the yeast Yarrowia lipolytica as a fusion protein

K. E. Cole, K. Geoghegan, A. Lanzetti, 1996 .

Identification of the residue responsible for catalysing regeneration of activity in the inactive glutamate dehydrogenase mutant D165N

P. Engel, K. Geoghegan, F. Paradisi, 2005, FEBS letters.

Plasminogen Activator Inhibitor-1

E. Goldsmith, D. Danley, J. Mottonen, 2003 .

Modification of Amino Groups

K. Geoghegan, 2016, Current protocols in protein science.

Cyclization of N-terminal S-carbamoylmethylcysteine causing loss of 17 Da from peptides and extra peaks in peptide maps.

J. Boyd, K. Geoghegan, L. R. Hoth, 2002, Journal of proteome research.

Isolation and characterization of biologically active murine interleukin-1α derived from expression of a synthetic gene in Escherichia coli

I. Otterness, K. Geoghegan, A. Mccoll, 1989 .

Cryospectroscopy of intermediates in the mechanism of carboxypeptidase A.

B. Vallee, K. Geoghegan, D. Auld, 1983, Biochemistry.

Spectral properties of cobalt carboxypeptidase A. Interaction of the metal atom with anions.

B. Vallee, K. Geoghegan, B. Holmquist, 1983, Biochemistry.

Alternative reducing agents for reductive methylation of amino groups in proteins.

K. Geoghegan, H. Dixon, R. Feeney, 2009, International journal of peptide and protein research.

Synthesis and biological activity of selective pipecolic acid-based TNF-alpha converting enzyme (TACE) inhibitors.

Dennis E Danley, D. Danley, G. Schulte, 2002, Bioorganic & medicinal chemistry letters.

Crystal structure of cholesteryl ester transfer protein reveals a long tunnel and four bound lipid molecules

Ing-Kae Wang, Xiayang Qiu, Dennis E Danley, 2007, Nature Structural &Molecular Biology.

Distinctive attributes of β-lactam target proteins in Acinetobacter baumannii relevant to development of new antibiotics.

V. Shanmugasundaram, K. Geoghegan, Xidong Feng, 2011, Journal of the American Chemical Society.

Chemical Proteomic Technologies for Drug Target Identification

K. Geoghegan, Douglas S. Johnson, 2010 .

Chemoproteomics demonstrates target engagement and exquisite selectivity of the clinical phosphodiesterase 10A inhibitor MP-10 in its native environment.

N. Brandon, P. Verhoest, C. J. Schmidt, 2014, ACS chemical biology.

Reduction of biological activity of murine recombinant interleukin‐1β by selective deamidation at asparagine‐149

I. Otterness, K. Geoghegan, A. Mccoll, 1991, FEBS letters.

The discovery of four distinct glutamate dehydrogenase genes in a strain of Halobacterium salinarum.

P. Engel, K. Geoghegan, B. M. Hayden, 2005, Gene.

Phosphorylation of serine residues in histidine-tag sequences attached to recombinant protein kinases: a cause of heterogeneity in mass and complications in function.

P. Lemotte, K. Coleman, K. Geoghegan, 2005, Protein expression and purification.

Biochemical applications of mass spectrometry in pharmaceutical drug discovery.

K. Geoghegan, M. Kelly, 2005, Mass spectrometry reviews.

Tandem mass spectrometry of multiply phosphorylated forms of a 'histidine-tag' derived from a recombinant protein kinase expressed in bacteria.

K. Geoghegan, Samuel P. Simons, P. Du, 2005, Rapid communications in mass spectrometry : RCM.

Engineered stabilization and structural analysis of the autoinhibited conformation of PDE4

J. Pandit, K. Geoghegan, A. Aulabaugh, 2015, Proceedings of the National Academy of Sciences.

Periodate inactivation of ovotransferrin and human serum transferrin.

K. Geoghegan, R. Feeney, J. Dallas, 1980, The Journal of biological chemistry.

Selective stalling of human translation through small-molecule engagement of the ribosome nascent chain

J. Doudna, J. Cate, P. Loria, 2017, PLoS biology.

Structural and biophysical studies of PCSK9 and its mutants linked to familial hypercholesterolemia

Xiayang Qiu, Dennis E Danley, S. Shenolikar, 2007, Nature Structural &Molecular Biology.

Antifreeze glycoproteins from Polar fish. Effects of freezing conditions on cooperative function.

D. Mulvihill, Y. Yeh, K. Geoghegan, 1980, The Journal of biological chemistry.

Deconstruction of activity-dependent covalent modification of heme in human neutrophil myeloperoxidase by multistage mass spectrometry (MS(4)).

K. Geoghegan, F. Vajdos, Xidong Feng, 2012, Biochemistry.

A Thermally Sensitive Loop in Clostridial Glutamate Dehydrogenase Detected by Limited Proteolysis*

P. Engel, K. Geoghegan, B. Chrunyk, 2003, The Journal of Biological Chemistry.

Antifreeze glycoproteins from Polar fish. Structural requirements for function of glycopeptide 8.

Y. Yeh, K. Geoghegan, R. Feeney, 1980, The Journal of biological chemistry.

X-ray analysis of HIV-1 proteinase at 2.7 Å resolution confirms structural homology among retroviral enzymes

John P. Overington, John Overington, Tom Blundell, 1989, Nature.

Selective Mechanism-Based Inactivation of CYP3A4 by CYP3cide (PF-04981517) and Its Utility as an In Vitro Tool for Delineating the Relative Roles of CYP3A4 versus CYP3A5 in the Metabolism of Drugs

G. S. Walker, R. Obach, R. Walsky, 2012, Drug Metabolism and Disposition.

Phosphodiesterase 9A Regulates Central cGMP and Modulates Responses to Cholinergic and Monoaminergic Perturbation In Vivo

Anne W. Schmidt, F. Menniti, M. Hajós, 2012, Journal of Pharmacology and Experimental Therapeutics.

Chemoproteomic profiling reveals that cathepsin D off-target activity drives ocular toxicity of β-secretase inhibitors

Graham M. West, Sherry L. Niessen, Claude Ambroise, 2016, Nature Communications.

Mapping the Binding Site of BMS-708163 on γ-Secretase with Cleavable Photoprobes.

K. Geoghegan, Christopher W. am Ende, Douglas S. Johnson, 2017, Cell chemical biology.

Glutathione S-Transferase Omega 1-1 Is a Target of Cytokine Release Inhibitory Drugs and May Be Responsible for Their Effect on Interleukin-1β Posttranslational Processing*

C. Gabel, K. Geoghegan, P. Rosner, 2003, The Journal of Biological Chemistry.

A compound that directly and selectively stalls PCSK9 translation

J. Doudna, J. Cate, P. Loria, 2016, bioRxiv.

Title : A compound that directly and selectively stalls PCSK 9 translation 1 2

J. Cate, P. Loria, S. Liras, 2016 .

Rapid-scanning cryospectroscopy of enzyme-substrate-inhibitor complexes of cobalt carboxypeptidase A.

B. Vallee, K. Geoghegan, D. Auld, 1986, Biochemistry.

Concentration-Dependent Modulation of Amyloid-β in Vivo and in Vitro Using the γ-Secretase Inhibitor, LY-450139

Tracy M. Brown, L. Lau, K. Geoghegan, 2006, Journal of Pharmacology and Experimental Therapeutics.

Concentration-dependent modulation of amyloid-beta in vivo and in vitro using the gamma-secretase inhibitor, LY-450139.

Tracy M. Brown, L. Lau, K. Geoghegan, 2006, The Journal of pharmacology and experimental therapeutics.

Fluorescence‐based continuous assay for the aspartyl protease of human immunodeficiency virus‐1

D. Danley, K. Geoghegan, R. Spencer, 1990, FEBS letters.

Site-directed conjugation of nonpeptide groups to peptides and proteins via periodate oxidation of a 2-amino alcohol. Application to modification at N-terminal serine.

K. Geoghegan, J. Stroh, 1992, Bioconjugate chemistry.

Development and characterization of a Fab fragment as a surrogate for the IL-1 receptor

T. Hynes, D. Wasilko, I. Otterness, 1997 .

Activation of Liver AMPK with PF-06409577 Corrects NAFLD and Lowers Cholesterol in Rodent and Primate Preclinical Models

M. Birnbaum, Jake E. Delmore, A. Kalgutkar, 2018, EBioMedicine.

Expression and secretion of bovine prochymosin in Yarrowia lipolytica

D. Danley, K. Geoghegan, L. Davidow, 1988 .

Initiation of translation at an upstream non-AUG codon accounting for N-terminally extended minor forms of recombinant proteins expressed in insect cells.

Paul W. Wu, K. Geoghegan, Xidong Feng, 2011, Protein expression and purification.

Modification of Amino Groups

K. Geoghegan, 1996, Current protocols in protein science.

Pharmacodynamics and Pharmacokinetics of the γ-Secretase Inhibitor PF-3084014

Carol D. Hicks, Tracy M. Brown, Douglas E. Wood, 2010, Journal of Pharmacology and Experimental Therapeutics.

Glutathione s-transferase omega 1-1 is a target of cytokine release inhibitory drugs and may be responsible for their effect on interleukin-1beta posttranslational processing.

C. Gabel, K. Geoghegan, P. Rosner, 2003, The Journal of biological chemistry.

Mutation of a Protease-sensitive Region in Firefly Luciferase Alters Light Emission Properties*

Johnf . Thompson, Shannon M. Anderson, K. Geoghegan, 1997, The Journal of Biological Chemistry.

Investigating γ-secretase protein interactions in live cells using active site-directed clickable dual-photoaffinity probes

K. Geoghegan, Douglas S. Johnson, T. E. Ballard, 2014 .

Expression of human plasminogen activator inhibitor type-1 (PAI-1) in Escherichia coli as a soluble protein comprised of active and latent forms. Isolation and crystallization of latent PAI-1.

D. Danley, R. Gerard, K. Geoghegan, 1990, Biochimica et biophysica acta.

Unexpected mucin-type O-glycosylation and host-specific N-glycosylation of human recombinant interleukin-17A expressed in a human kidney cell line.

Xi Song, Xidong Feng, K. Geoghegan, 2013, Protein expression and purification.

Site-directed double fluorescent tagging of human renin and collagenase (MMP-1) substrate peptides using the periodate oxidation of N-terminal serine. An apparently general strategy for provision of energy-transfer substrates for proteases.

K. Geoghegan, A. Mccoll, G. Daumy, 1993, Bioconjugate chemistry.

Phosphoproteomic evaluation of pharmacological inhibition of leucine‐rich repeat kinase 2 reveals significant off‐target effects of LRRK‐2‐IN‐1

Chuong Nguyen, Hualin Xi, H. Xi, 2014, Journal of neurochemistry.

Design and chemoproteomic functional characterization of a chemical probe targeted to bromodomains of BET family proteins

S. Wright, K. Geoghegan, P. Sahasrabudhe, 2014 .

A continuous and direct assay to monitor leucine-rich repeat kinase 2 activity.

K. Geoghegan, A. Aulabaugh, R. G. Silva, 2014, Analytical biochemistry.

Pharmacodynamics and Pharmacokinetics of the -Secretase Inhibitor PF-3084014 □ S

Carol D. Hicks, Tracy M. Brown, Douglas E. Wood, 2010 .

Concentration-Dependent Modulation of Amyloid- (cid:1) in Vivo and in Vitro Using the (cid:2) -Secretase Inhibitor, LY-450139

Tracy M. Brown, L. Lau, K. Geoghegan, 2006 .

Discovery of a Selective Covalent Inhibitor of Lysophospholipase-like 1 (LYPLAL1) as a Tool to Evaluate the Role of this Serine Hydrolase in Metabolism.

K. Fennell, J. Pandit, K. Parris, 2016, ACS chemical biology.

Structural basis of latency in plasminogen activator inhibitor-1

E. Goldsmith, D. Danley, J. Symerský, 1992, Nature.

Cloning, expression, and type II collagenolytic activity of matrix metalloproteinase-13 from human osteoarthritic cartilage.

K. Geoghegan, P. Rosner, H. Magna, 1996, The Journal of clinical investigation.

Spontaneous chemical reversion of an active site mutation: deamidation of an asparagine residue replacing the catalytic aspartic acid of glutamate dehydrogenase.

P. Engel, J. Dean, K. Geoghegan, 2005, Biochemistry.

Hydrolysis of peptides by carboxypeptidase A: equilibrium trapping of the ES2 intermediate.

K. Geoghegan, G. Hanson, D. Auld, 1986, Biochemistry.

IL-1 beta maturation: evidence that mature cytokine formation can be induced specifically by nigericin.

C. Gabel, K. Geoghegan, A. Lanzetti, 1992, Journal of immunology.

Structural basis for AMPK activation: natural and synthetic ligands regulate kinase activity from opposite poles by different molecular mechanisms.

R. Kurumbail, M. Harris, Hong Wang, 2014, Structure.

Cryospectrokinetic characterization of intermediates in biochemical reactions: carboxypeptidase A.

K. Geoghegan, D. Auld, A. Galdes, 1984, Proceedings of the National Academy of Sciences of the United States of America.

Deconstruction of activity-dependent covalent modification of heme in human neutrophil myeloperoxidase by multistage mass spectrometry (MS(4)).

K. Geoghegan, F. Vajdos, Xidong Feng, 2012, Biochemistry.

Unexpected mucin-type O-glycosylation and host-specific N-glycosylation of human recombinant interleukin-17A expressed in a human kidney cell line.

K. Geoghegan, L. R. Hoth, D. Luxenberg, 2013, Protein expression and purification.