Jay D Keasling

发表

Synergies between synthetic biology and metabolic engineering

Jens Nielsen, Jay D Keasling, J. Keasling, 2011, Nature Biotechnology.

Metabolic engineering delivers next-generation biofuels

Jay D Keasling, Howard Chou, J. Keasling, 2008, Nature Biotechnology.

The Experiment Data Depot: A Web-Based Software Tool for Biological Experimental Data Storage, Sharing, and Visualization.

Nathan J Hillson, David Ando, Tyler W. H. Backman, 2017, ACS synthetic biology.

BglBricks: A flexible standard for biological part assembly

Jay D Keasling, J Christopher Anderson, John E Dueber, 2010, Journal of biological engineering.

Narrowing the gap between the promise and reality of polyketide synthases as a synthetic biology platform.

Jay D Keasling, Leonard Katz, Sean Poust, 2014, Current opinion in biotechnology.

j5 DNA assembly design automation software.

Nathan J Hillson, Jay D Keasling, Rafael D Rosengarten, 2012, ACS synthetic biology.

A framework and model system to investigate linear system behavior in Escherichia coli

Meghdad Hajimorad, Jay D Keasling, Paul R Gray, 2011, Journal of biological engineering.

Addressing the need for alternative transportation fuels: the Joint BioEnergy Institute.

Jay D Keasling, Paul D Adams, Blake A Simmons, 2008, ACS chemical biology.

Programming cellular function

Jay D Keasling, Christopher A Voigt, J. Keasling, 2005, Nature chemical biology.

Commodity Chemicals From Engineered Modular Type I Polyketide Synthases.

Jay D Keasling, Amin Zargar, Bo Pang, 2018, Methods in enzymology.

Development of biosensors and their application in metabolic engineering.

Jay D Keasling, Jie Zhang, Michael K Jensen, 2015, Current opinion in chemical biology.

PaR-PaR laboratory automation platform.

Nathan J Hillson, Gregory Linshiz, Jay D Keasling, 2013, ACS synthetic biology.

Combining mechanistic and machine learning models for predictive engineering and optimization of tryptophan metabolism

Jay D Keasling, Jens Nielsen, Zak Costello, 2020, Nature Communications.

Engineering of Bacterial Methyl Ketone Synthesis for Biofuels

Ee-Been Goh, Harry R Beller, Jay D Keasling, 2012, Applied and Environmental Microbiology.

Building a global alliance of biofoundries

Douglas C. Friedman, Huimin Zhao, Filippo Menolascina, 2019, Nature Communications.

DeviceEditor visual biological CAD canvas

Nathan J Hillson, Douglas Densmore, Jay D Keasling, 2012, Journal of Biological Engineering.

PR-PR: cross-platform laboratory automation system.

Nathan J Hillson, Garima Goyal, Monica Sharma, 2014, ACS synthetic biology.

Metabolic engineering of microorganisms for isoprenoid production.

Jay D Keasling, James Kirby, J. Keasling, 2008, Natural product reports.

Pathway engineering by designed divergent evolution.

Jay D Keasling, Yasuo Yoshikuni, J. Keasling, 2007, Current opinion in chemical biology.

Charge-associated effects of fullerene derivatives on microbial structural integrity and central metabolism.

Chul-Hyun Kim, Yinjie J. Tang, Jay D Keasling, 2007, Nano letters.

A novel semi-biosynthetic route for artemisinin production using engineered substrate-promiscuous P450(BM3).

David Baker, Jay D Keasling, Yasuo Yoshikuni, 2009, ACS chemical biology.

Coupling S-adenosylmethionine–dependent methylation to growth: Design and uses

Adam M. Feist, Bernhard O Palsson, Jay D Keasling, 2019, PLoS biology.

Microbioreactor arrays with parametric control for high‐throughput experimentation

Jay D Keasling, Michel M Maharbiz, Roger T Howe, 2004, Biotechnology and bioengineering.

Engineering a Polyketide Synthase for In Vitro Production of Adipic Acid.

Jay D Keasling, Christopher J Petzold, Andrew Hagen, 2016, ACS synthetic biology.

Transcriptomic and proteomic analyses of Desulfovibrio vulgaris biofilms: Carbon and energy flow contribute to the distinct biofilm growth state

Jay D Keasling, Aindrila Mukhopadhyay, Adam P Arkin, 2012, BMC Genomics.

Quantitative proteomic profiling of host-pathogen interactions: the macrophage response to Mycobacterium tuberculosis lipids.

Jay D Keasling, Wenqing Shui, Carolyn R Bertozzi, 2009, Journal of proteome research.

Amplification of HMG-CoA reductase production enhances carotenoid accumulation in Neurospora crassa.

Jay D Keasling, J. Keasling, Guang-Yi Wang, 2002, Metabolic engineering.

Model-Driven Engineering of RNA Devices to Quantitatively Program Gene Expression

Jay D Keasling, James M Carothers, Jonathan A Goler, 2011, Science.

Optimization of the mevalonate-based isoprenoid biosynthetic pathway in Escherichia coli for production of the anti-malarial drug precursor amorpha-4,11-diene.

Jay D Keasling, J. Keasling, J. Newman, 2009, Metabolic engineering.

Tracing Determinants of Dual Substrate Specificity in Glycoside Hydrolase Family 5*

Dylan Chivian, Jay D Keasling, Paul D Adams, 2012, The Journal of Biological Chemistry.

CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae

Jay D Keasling, Irina Borodina, Alex Toftgaard Nielsen, 2015, Microbial Cell Factories.

Genome-scale metabolic rewiring improves titers rates and yields of the non-native product indigoidine at scale

Thomas Eng, Jay D Keasling, Christopher J Petzold, 2020, Nature Communications.

Design, Engineering, and Characterization of Prokaryotic Ligand-Binding Transcriptional Activators as Biosensors in Yeast.

Jay D Keasling, Michael K Jensen, Tim Snoek, 2018, Methods in molecular biology.

Isotopomer distributions in amino acids from a highly expressed protein as a proxy for those from total protein.

Yinjie J. Tang, Jay D Keasling, Aindrila Mukhopadhyay, 2008, Analytical chemistry.

Effect of lacYExpression on Homogeneity of Induction from the Ptac and Ptrc Promoters by Natural and Synthetic Inducers

Jay D Keasling, J. Keasling, A. Khlebnikov, 2002, Biotechnology progress.

Evaluation of the effects of various culture conditions on Cr(VI) reduction by Shewanella oneidensis MR‐1 in a novel high‐throughput mini‐bioreactor

Yinjie J. Tang, Jay D Keasling, Yinjie J Tang, 2006, Biotechnology and bioengineering.

Evolution-guided engineering of small-molecule biosensors

Jay D Keasling, Stefan Kol, Michael K Jensen, 2019, Nucleic acids research.

Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae for high-level production of isoprenoids.

Jay D Keasling, James Kirby, J. Keasling, 2007, Metabolic engineering.

Mevalonate Pathway Promiscuity Enables Noncanonical Terpene Production.

Nathan J Hillson, Oliver Fiehn, Jay D Keasling, 2019, ACS synthetic biology.

Development of a Transcription Factor-Based Lactam Biosensor.

Jay D Keasling, Thomas L Ruegg, J. Keasling, 2017, ACS synthetic biology.

Analysis of metabolic pathways and fluxes in a newly discovered thermophilic and ethanol‐tolerant Geobacillus strain

Yinjie J. Tang, Jay D Keasling, Yinjie J Tang, 2009, Biotechnology and bioengineering.

Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer

Jay D Keasling, Christopher J Petzold, Zak Costello, 2018, Nature Communications.

Metabolic flux elucidation for large-scale models using 13C labeled isotopes.

Madhukar S. Dasika, Jay D Keasling, Patrick F Suthers, 2007, Metabolic engineering.

A kinetic model describing Shewanella oneidensis MR‐1 growth, substrate consumption, and product secretion

Yinjie J. Tang, Jay D Keasling, Yinjie J Tang, 2007, Biotechnology and bioengineering.

Effect of gene location, mRNA secondary structures, and RNase sites on expression of two genes in an engineered operon.

Jay D Keasling, Christina D Smolke, J. Keasling, 2002, Biotechnology and bioengineering.

Microbial sensors for small molecules: development of a mevalonate biosensor.

Jay D Keasling, Brian F Pfleger, Brian F. Pfleger, 2007, Metabolic engineering.

Development of Next Generation Synthetic Biology Tools for Use in Streptomyces venezuelae.

Jay D Keasling, Leonard Katz, Blake J. Rasor, 2017, ACS synthetic biology.

De novo DNA synthesis using polymerase-nucleotide conjugates

Nathan J Hillson, Jay D Keasling, J. Keasling, 2018, Nature Biotechnology.

A Droplet Microfluidic Platform for Automating Genetic Engineering.

Nathan J Hillson, Steve C. C. Shih, Jay D Keasling, 2016, ACS synthetic biology.

OpenMSI Arrayed Analysis Toolkit: Analyzing Spatially Defined Samples Using Mass Spectrometry Imaging.

Oliver Rübel, Benjamin P Bowen, Jay D Keasling, 2017, Analytical chemistry.

Induction of multiple pleiotropic drug resistance genes in yeast engineered to produce an increased level of anti-malarial drug precursor, artemisinic acid

Jay D Keasling, J. Keasling, D. Ro, 2008, BMC biotechnology.

Heterologous Gene Expression of N-Terminally Truncated Variants of LipPks1 Suggests a Functionally Critical Structural Motif in the N-terminus of Modular Polyketide Synthase.

Constance B. Bailey, Jay D Keasling, Christopher J Petzold, 2017, ACS chemical biology.

A kinetic‐based approach to understanding heterologous mevalonate pathway function in E. coli

Edward Baidoo, Jay D Keasling, Christopher J Petzold, 2015, Biotechnology and bioengineering.

Increased β‐Carotene Production in Recombinant Escherichia coli Harboring an Engineered Isoprenoid Precursor Pathway with Mevalonate Addition

Jay D Keasling, Seon-Won Kim, Sang-Hwal Yoon, 2008, Biotechnology progress.

Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli.

Jay D Keasling, J. Keasling, D. Pitera, 2007, Metabolic engineering.

Divergent mechanistic routes for the formation of gem-dimethyl groups in the biosynthesis of complex polyketides.

Jay D Keasling, Christopher J Petzold, Leonard Katz, 2015, Angewandte Chemie.

Alteration of Polyketide Stereochemistry from anti to syn by a Ketoreductase Domain Exchange in a Type I Modular Polyketide Synthase Subunit.

Jay D Keasling, Satoshi Yuzawa, Clara H. Eng, 2016, Biochemistry.

Comprehensive Structural and Biochemical Analysis of the Terminal Myxalamid Reductase Domain for the Engineered Production of Primary Alcohols.

Ray Luo, Jay D Keasling, Shiou-Chuan Tsai, 2015, Chemistry & biology.

Programming adaptive control to evolve increased metabolite production

Jay D Keasling, Howard H Chou, J. Keasling, 2013, Nature Communications.

Enhanced lycopene production in Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate

Jay D Keasling, Seon-Won Kim, Sang-Hwal Yoon, 2006, Biotechnology and bioengineering.

A three-dimensional, stochastic simulation of biofilm growth and transport-related factors that affect structure.

Jay D Keasling, Eric S Gilbert, J. Keasling, 2003, Microbiology.

Heterologous production of polyketides by modular type I polyketide synthases in Escherichia coli.

Jay D Keasling, Satoshi Yuzawa, Woncheol Kim, 2012, Current opinion in biotechnology.

In vitro analysis of carboxyacyl substrate tolerance in the loading and first extension modules of borrelidin polyketide synthase.

Jay D Keasling, Christopher J Petzold, Paul D Adams, 2014, Biochemistry.

Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production.

Jay D Keasling, Christopher J Petzold, Paul D Adams, 2013, Metabolic engineering.

Microbioreactor arrays with parametric control for high-throughput experimentation.

Jay D Keasling, Michel M Maharbiz, Roger T Howe, 2004, Biotechnology and bioengineering.

Engineering Escherichia coli for production of functionalized terpenoids using plant P450s.

Jay D Keasling, J. Keasling, D. Ro, 2007, Nature chemical biology.

Production of isoprenoid pharmaceuticals by engineered microbes

Jay D Keasling, J. Keasling, Michelle C. Y. Chang, 2006, Nature chemical biology.

Combinatorial expression of bacterial whole mevalonate pathway for the production of beta-carotene in E. coli.

Jay D Keasling, Seon-Won Kim, Hee-Jeong Jang, 2009, Journal of biotechnology.

Substantial improvements in methyl ketone production in E. coli and insights on the pathway from in vitro studies.

Ee-Been Goh, Harry R Beller, Jay D Keasling, 2014, Metabolic engineering.

Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae.

Jay D Keasling, Markus J. Herrgård, Mette Kristensen, 2015, Metabolic engineering.

Developing Aspergillus as a host for heterologous expression.

Jay D Keasling, J. Keasling, David Lubertozzi, 2009, Biotechnology advances.

Precise and reliable gene expression via standard transcription and translation initiation elements

Drew Endy, Jay D Keasling, Adam P Arkin, 2013, Nature Methods.

Redirection of flux through the FPP branch‐point in Saccharomyces cerevisiae by down‐regulating squalene synthase

Jay D Keasling, James Kirby, J. Keasling, 2008, Biotechnology and bioengineering.

Enhancing fatty acid production by the expression of the regulatory transcription factor FadR.

Jay D Keasling, Christopher J Petzold, Aindrila Mukhopadhyay, 2012, Metabolic engineering.

Biosynthesis and incorporation of side-chain-truncated lignin monomers to reduce lignin polymerization and enhance saccharification.

Seema Singh, Jay D Keasling, Fan Yang, 2012, Plant biotechnology journal.

Broad substrate specificity of the loading didomain of the lipomycin polyketide synthase.

Jay D Keasling, Satoshi Yuzawa, Clara H. Eng, 2013, Biochemistry.

Microbial synthesis of pinene.

Jay D Keasling, Pamela Peralta-Yahya, J. Keasling, 2014, ACS synthetic biology.

Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway

Jay D Keasling, Eui-Sung Choi, Seon-Won Kim, 2010, Biotechnology and bioengineering.

Redesigning enzymes based on adaptive evolution for optimal function in synthetic metabolic pathways.

Jay D Keasling, Patricia C Babbitt, Yasuo Yoshikuni, 2008, Chemistry & biology.

Transcription factor-based screens and synthetic selections for microbial small-molecule biosynthesis.

Jay D Keasling, David L Shis, J. Keasling, 2013, ACS synthetic biology.

Leveraging microbial biosynthetic pathways for the generation of 'drop-in' biofuels.

Constance B. Bailey, Jay D Keasling, Amin Zargar, 2017, Current opinion in biotechnology.

Metabolic engineering of microbial pathways for advanced biofuels production.

Jay D Keasling, Fuzhong Zhang, Sarah Rodriguez, 2011, Current opinion in biotechnology.

Comprehensive in Vitro Analysis of Acyltransferase Domain Exchanges in Modular Polyketide Synthases and Its Application for Short-Chain Ketone Production.

Jay D Keasling, Paul D Adams, Trent R Northen, 2017, ACS synthetic biology.

Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production.

Jay D Keasling, Taek Soon Lee, J. Keasling, 2013, Metabolic engineering.

Engineering a functional 1-deoxy-D-xylulose 5-phosphate (DXP) pathway in Saccharomyces cerevisiae.

Peter Jackson, Jay D Keasling, Kristy M. Hawkins, 2016, Metabolic engineering.

Improved assembly of multimeric genes for the biosynthetic production of protein polymers.

Jay D Keasling, J. Keasling, S. Muller, 2002, Biomacromolecules.

BglBrick vectors and datasheets: A synthetic biology platform for gene expression

Meghdad Hajimorad, Jay D Keasling, Fuzhong Zhang, 2011, Journal of biological engineering.

Enzyme analysis of the polyketide synthase leads to the discovery of a novel analog of the antibiotic α-lipomycin

Jay D Keasling, Satoshi Yuzawa, Clara H. Eng, 2013, The Journal of Antibiotics.

Dual-selection for evolution of in vivo functional aptazymes as riboswitch parts.

Jay D Keasling, James M Carothers, Jonathan A Goler, 2014, Methods in molecular biology.

Process design for microbial plastic factories: metabolic engineering of polyhydroxyalkanoates.

Jay D Keasling, J. Keasling, Ilana S Aldor, 2003, Current opinion in biotechnology.

CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae.

Jay D Keasling, Irina Borodina, Jie Zhang, 2015, ACS synthetic biology.

High‐level production of amorpha‐4,11‐diene in a two‐phase partitioning bioreactor of metabolically engineered Escherichia coli

Jay D Keasling, J. Keasling, Eric M. Paradise, 2006, Biotechnology and bioengineering.

Biochemical Characterization of β‐Amino Acid Incorporation in Fluvirucin B2 Biosynthesis

Nathan J Hillson, Jay D Keasling, Amin Zargar, 2018, Chembiochem : a European journal of chemical biology.

An Orthogonal and pH-Tunable Sensor-Selector for Muconic Acid Biosynthesis in Yeast.

Jay D Keasling, Jie Zhang, Michael K Jensen, 2018, ACS synthetic biology.

Engineered reversal of function in glycolytic yeast promoters

Jay D Keasling, Jiufu Qin, Emre Özdemir, 2019, bioRxiv.

Bio-based production of fuels and industrial chemicals by repurposing antibiotic-producing type I modular polyketide synthases: opportunities and challenges

Jay D Keasling, Satoshi Yuzawa, Leonard Katz, 2016, The Journal of Antibiotics.

Development of an orthogonal fatty acid biosynthesis system in E. coli for oleochemical production.

Jay D Keasling, Leonard Katz, Simon F Brunner, 2015, Metabolic engineering.

Engineering an NADPH/NADP+ Redox Biosensor in Yeast.

Nikolaus Sonnenschein, Jay D Keasling, Jie Zhang, 2016, ACS synthetic biology.

Invariability of central metabolic flux distribution in Shewanella oneidensis MR‐1 under environmental or genetic perturbations

Xueyang Feng, Yinjie J. Tang, Jay D Keasling, 2009, Biotechnology progress.

Importance of systems biology in engineering microbes for biofuel production.

Jay D Keasling, Aindrila Mukhopadhyay, Alyssa M. Redding, 2008, Current opinion in biotechnology.

System-level perturbations of cell metabolism using CRISPR/Cas9.

Jay D Keasling, Michael K Jensen, Tadas Jakočiūnas, 2017, Current opinion in biotechnology.

Separation and mass spectrometry in microbial metabolomics.

Yinjie J. Tang, Jay D Keasling, Yinjie J Tang, 2008, Current opinion in microbiology.

Effect of copy number and mRNA processing and stabilization on transcript and protein levels from an engineered dual-gene operon.

Jay D Keasling, Christina D Smolke, J. Keasling, 2002, Biotechnology and bioengineering.

Biofuel alternatives to ethanol: pumping the microbial well.

Swapnil Chhabra, Jay D Keasling, Howard Chou, 2008, Trends in biotechnology.

Study of stationary phase metabolism via isotopomer analysis of amino acids from an isolated protein

Yinjie J. Tang, Héctor García Martín, Jay D Keasling, 2010, Biotechnology progress.

Advanced biofuel production in microbes

Jay D Keasling, J. Keasling, P. Peralta-Yahya, 2010, Biotechnology journal.

Short-chain ketone production by engineered polyketide synthases in Streptomyces albus

Jay D Keasling, Satoshi Yuzawa, Blake A Simmons, 2018, Nature Communications.