I. Hirao

发表

Crystal structures of a series of RNA aptamers complexed to the same protein target

A. Ellington, S. Phillips, P. Stockley, 1998, Nature Structural Biology.

Generation of high-affinity DNA aptamers using an expanded genetic alphabet

S. Yokoyama, I. Hirao, M. Kimoto, 2013, Nature Biotechnology.

Sanger Gap Sequencing for Genetic Alphabet Expansion of DNA.

I. Hirao, M. Kimoto, Si Hui Gabriella Soh, 2020, Chembiochem : a European journal of chemical biology.

Cognate base‐pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase

I. Hirao, I. Okamoto, M. Kimoto, 2020, Biopolymers.

DNA Sequencing Method Including Unnatural Bases for DNA Aptamer Generation by Genetic Alphabet Expansion.

K. Hamashima, I. Hirao, M. Kimoto, 2019, ACS synthetic biology.

Recent advances in synthetic biosafety

A. Ellington, I. Hirao, Anna J. Simon, 2016, F1000Research.

Genetic Alphabet Expansion Provides Versatile Specificities and Activities of Unnatural-Base DNA Aptamers Targeting Cancer Cells

I. Hirao, M. Kimoto, K. Futami, 2018, Molecular therapy. Nucleic acids.

Unnatural base pairs between 2- and 6-substituted purines and 2-oxo(1H)pyridine for expansion of the genetic alphabet.

S. Yokoyama, T. Fujiwara, I. Hirao, 2004, Bioorganic & medicinal chemistry letters.

A unique unnatural base pair between a C analogue, pseudoisocytosine, and an A analogue, 6-methoxypurine, in replication.

S. Yokoyama, I. Hirao, M. Kimoto, 2002, Bioorganic & medicinal chemistry letters.

Synthesis of 6-(2-thienyl)purine nucleoside derivatives that form unnatural base pairs with pyridin-2-one nucleosides.

S. Yokoyama, H. Sugiyama, T. Fujiwara, 2001, Bioorganic & medicinal chemistry letters.

Molecular affinity rulers: systematic evaluation of DNA aptamers for their applicabilities in ELISA

I. Hirao, M. Kimoto, Yun Wei Shermane Lim, 2019, Nucleic acids research.

Crystal structure of d(GCGAAAGCT) containing a parallel-stranded duplex with homo base pairs and an anti-parallel duplex with Watson-Crick base pairs.

J. Kondo, K. Miura, I. Hirao, 2002, Nucleic acids research.

Success probability of high-affinity DNA aptamer generation by genetic alphabet expansion

Y. S. Tan, I. Hirao, M. Kimoto, 2022, bioRxiv.

Human cystatin A is inactivated by engineered truncation. The NH2-terminal region of the cysteine proteinase inhibitor is essential for expression of its inhibitory activity.

T. Itoh, I. Kumagai, K. Miura, 1995, Biochemistry.

Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamers

Y. Leo, S. Vasoo, I. Hirao, 2021, Scientific Reports.

Unnatural base pairs between 2-amino-6-(2-thienyl)purine and the complementary bases.

T. Ohtsuki, S. Yokoyama, T. Fujiwara, 2000, Nucleic acids symposium series.

Structure of d(GCGAAAGC) (hexagonal form): a base-intercalated duplex as a stable structure.

J. Kondo, K. Miura, I. Hirao, 2004, Acta crystallographica. Section D, Biological crystallography.

Synthetic Genetic Polymers Functioning to Store and Propagate Information by Genetic Alphabet Expansion

I. Hirao, 2014 .

The Effect of Hairpin DNA Fragments on Escherichia coli Poly(U)-Dependent Poly(Phe) Synthesis

K. Miura, S. Yoshizawa, I. Hirao, 2000 .

Nuclease resistance of an extraordinarily thermostable mini-hairpin DNA fragment, d(GCGAAGC) and its application to in vitro protein synthesis.

K. Watanabe, K. Miura, T. Ueda, 1994, Nucleic acids research.

The Use of Hydroxybenzoic Acids to Polymers. II. Polyether-amides Obtained from the Interfacial Polycondensation of p -Carboxyphenoxyacetic Acid Dichloride with Aliphatic Diamines

I. Hirao, T. Fujimoto, T. Kito, 1974 .

Genetic Code Engineering by Natural and Unnatural Base Pair Systems for the Site-Specific Incorporation of Non-Standard Amino Acids Into Proteins

I. Hirao, M. Kimoto, 2022, Frontiers in Molecular Biosciences.

Site-Specific Incorporation of Functional Components into RNA by an Unnatural Base Pair Transcription System

I. Hirao, M. Kimoto, R. Kawai, 2012, Molecules.

A two-unnatural-base-pair system toward the expansion of the genetic code.

S. Yokoyama, T. Fujiwara, I. Hirao, 2004, Journal of the American Chemical Society.

An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules

Shigeyuki Yokoyama, Ichiro Hirao, Michiko Kimoto, 2008, Nucleic acids research.

Visual Detection of Amplified DNA by Polymerase Chain Reaction Using a Genetic Alphabet Expansion System.

I. Hirao, M. Kimoto, Rie Yamashige, 2018, Journal of the American Chemical Society.

Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants.

A. Ellington, I. Hirao, M. Kimoto, 2017, Chemical communications.

Structural Basis for Expansion of the Genetic Alphabet with an Artificial Nucleobase Pair.

K. Diederichs, A. Marx, I. Hirao, 2017, Angewandte Chemie.

Unnatural base pair systems for sensing and diagnostic applications

R. Cox, I. Hirao, M. Kimoto, 2011, Expert review of molecular diagnostics.

A unique fluorescent base analogue for the expansion of the genetic alphabet.

S. Yokoyama, I. Hirao, M. Kimoto, 2010, Journal of the American Chemical Society.

An efficient unnatural base pair for PCR amplification.

S. Yokoyama, I. Hirao, M. Kimoto, 2007, Journal of the American Chemical Society.

Site-specific fluorescent labeling of RNA molecules by specific transcription using unnatural base pairs.

S. Yokoyama, S. Ikeda, Masayuki Endo, 2005, Journal of the American Chemical Society.

Site-speci ® c incorporation of an unnatural amino acid into proteins in mammalian cells

Kazuki Saito, S. Yokoyama, K. Sakamoto, 2022 .

Effect of tandem repeated AUG codons on translation efficiency of eukaryotic mRNA carrying a short leader sequence

I. Kumagai, K. Miura, I. Hirao, 1993, Molecular and General Genetics MGG.

Oligonucleotide synthesis in terms of a novel type of polymer-support: a cellulose acetate functionalized with 4-(2-hydroxyethylsulfonyl)dihydrocinnamoyl substituent

K. Miura, I. Hirao, Kimitsuna Watanabe, 1990 .

Acetylenic Alcohol and its Derivatives. VII: Chemical Properties of 1-Amino-3-phenyl-propen-3-one and Synthesis of 5-Benzoyl-2-phenylpyridine@@@1-Amino-3-phenylpropen-3-oneの性質と5-Benzoyl-2-phenylpyridineの合成に就いて

I. Hirao, 1954 .

Characterization of fluorescent, unnatural base pairs

S. Yokoyama, I. Hirao, M. Kimoto, 2007 .

Unnatural base pair systems for DNA/RNA-based biotechnology.

Ichiro Hirao, I. Hirao, 2006, Current opinion in chemical biology.

An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA

Yoko Harada, Tsuyoshi Fujiwara, Shigeyuki Yokoyama, 2006, Nature Methods.

An unnatural hydrophobic base, 4-propynylpyrrole-2-carbaldehyde, as an efficient pairing partner of 9-methylimidazo[(4,5)-b]pyridine.

S. Yokoyama, I. Hirao, M. Kimoto, 2003, Bioorganic & medicinal chemistry letters.

An unnatural hydrophobic base pair with shape complementarity between pyrrole-2-carbaldehyde and 9-methylimidazo[(4,5)-b]pyridine.

Shigeyuki Yokoyama, Ichiro Hirao, Michiko Kimoto, 2003, Journal of the American Chemical Society.

Regioselective phenylcarbamoylation of purine and pyrimidine ribonucleosides through dibutyltin oxide-and tertiary amine-phenyl isocyanate systems

I. Hirao, Y. Ishido, Y. Araki, 1986 .

Partial Protection of Carbohydrate Derivatives. Part 14. Regioselective Phenylcarbamoylation of Purine and Pyrimidine Ribonucleosides Through Dibutyltin Oxide- and Tertiary Amine-Phenyl Isocyanate Systems.

I. Hirao, Y. Ishido, Y. Araki, 1987 .

Bis(tributyltin) oxide-phenyl isocyanate system for regioselective (phenylcarbamoyl)ation of purine and pyrimidine ribonucleosides☆

N. Sakairi, I. Hirao, Y. Ishido, 1982 .

Genetic alphabet expansion technology by creating unnatural base pairs.

Ichiro Hirao, Michiko Kimoto, I. Hirao, 2020, Chemical Society reviews.

An efficient method for the alkynylation of oxiranes using alkynyl boranes

M. Yamaguchi, I. Hirao, 1983 .

REACTION OF OXIRANES WITH DIANION OF METHYL ACETOACETATE IN THE PRESENCE OF BORON TRIFLUORIDE ETHERATE

M. Yamaguchi, I. Hirao, 1985 .

Synthesis and reactions of the (1-cyclobutenyl)triphenylphosphonium salt

T. Hanamoto, I. Hirao, T. Minami, 1983 .

Synthesis of a 3,1-Benzoxazin-4-one, 2,4(1H,3H)-Quinolinediones, and 2,4(1H,3H)-Quinazolinediones from the Reaction of Phosphoryl-Stabilized Anions Containing no a-Hydrogen Atoms with Isatoic Anhydride

M. Ogata, I. Hirao, T. Minami, 1982 .

Reactions of vinylphosphonates. 2. Synthesis of functionalized dienes, trienes, and their analogs. Synthetic applications to regioselectively functionalized benzene derivatives

I. Hirao, T. Minami, T. Agawa, 1982 .

A convenient synthesis of alkynyl ketones from esters

M. Yamaguchi, I. Hirao, Keisuke Shibato, 1986 .

Reactions of α-Lithiatd 4H-1-Benzothiopyran-4-one and 1,2-Dihydrocyclopenta[b][1]benzothiopyran-9(3H)-one Derivatives

M. Yamaguchi, I. Hirao, K. Hasebe, 1985 .

Cycloaddition of diazomethane to butadienylphosphonates. A new approach to functionalized pentadienylphosphonates and pyrazoles.

I. Hirao, T. Minami, Shigehumi Tokumasu, 1985 .

Synthesis of heterocyclic compounds containing phosphorus residues by cycloaddition of 1,3-dipoles to cyclobutenylphosphorus compounds

T. Hanamoto, I. Hirao, T. Minami, 1985 .

a highly stereoselective synthesis of carbocyclic compounds by the michael induced intramolecular alkylation a stereocontrol of extracyclic chiral centers

M. Yamaguchi, I. Hirao, M. Tsukamoto, 1985 .

A highly stereoselective Michael reaction of simple ester enolates to α,β-unsaturated esters

M. Yamaguchi, I. Hirao, Shinya Tanaka, 1985 .

A ring opening reaction of oxetanes with lithium acetylides promoted by boron trifluoride etherate

M. Yamaguchi, I. Hirao, Yukio Nobayashi, 1984 .

The alkynylation reaction of oxetanes

M. Yamaguchi, I. Hirao, Yukio Nobayashi, 1984 .

The Reaction of Butadienylphosphonates with a Oxosulfonium Ylide, Phosphonium Ylides, and Ketone Enolates

I. Hirao, T. Minami, Shigehumi Tokumasu, 1984 .

Synthesis of butadienylphosphonates containing electronegative substituents and their synthetic applications to functionalized cyclopentenylphosphonates

I. Hirao, T. Minami, S. Takenaka, 1983 .

Regioselective Phenylcarbamoylation of the Hydroxyl Groups of Purine and Pyrimidine Ribonucleosides with Bis(tributyltin)Oxide-Phenyl Isocyanate

N. Sakairi, I. Hirao, Y. Ishido, 1979 .

Chemical Conversion of Some Ribonucleosides into the Corresponding β-D-Arabinofuranosyl Derivatives1

N. Sakairi, I. Hirao, Y. Ishido, 1983 .

Natural versus artificial creation of base pairs in DNA: origin of nucleobases from the perspectives of unnatural base pair studies.

Ichiro Hirao, Michiko Kimoto, I. Hirao, 2012, Accounts of chemical research.

An unnatural base pair for incorporating amino acid analogs into proteins

Tsuyoshi Fujiwara, Takanori Kigawa, Shigeyuki Yokoyama, 2002, Nature Biotechnology.

Cell-Free Synthesis of Proteins with Unnatural Amino Acids. The PURE System and Expansion of the Genetic Code

T. Ueda, I. Hirao, T. Kanamori, 2009 .

Placing extra components into RNA by specific transcription using unnatural base pair systems.

I. Hirao, 2006, BioTechniques.

An engineered Escherichia coli tyrosyl–tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system

Daisuke Kiga, Yoko Harada, Takanori Kigawa, 2002, Proceedings of the National Academy of Sciences of the United States of America.

Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase

Dong Wang, J. Chong, J. Oh, 2023, Nature Communications.

Fluorescent probing for RNA molecules by an unnatural base-pair system

S. Yokoyama, I. Hirao, M. Kimoto, 2007, Nucleic acids research.

Site-specific labeling of RNA by combining genetic alphabet expansion transcription and copper-free click chemistry

I. Hirao, M. Kimoto, T. Someya, 2015, Nucleic acids research.

A new synthesis of δ-lactones from oxetanes

M. Yamaguchi, I. Hirao, Keisuke Shibato, 1984 .

Cytostatic evaluations of nucleoside analogs related to unnatural base pairs for a genetic expansion system.

S. Yokoyama, I. Hirao, M. Kimoto, 2007, Bioorganic & medicinal chemistry letters.

Site-specific incorporation of a photo-crosslinking component into RNA by T7 transcription mediated by unnatural base pairs.

S. Yokoyama, Masayuki Endo, I. Hirao, 2004, Chemistry & biology.

1, 1, 3, 3-Tetraisopropyl-3-(2-(triphenylmethoxy)ethoxy)disiloxane-1-yl group, a potential 5′-O-protecting group for solid-phase RNA synthesis

I. Hirao, M. Koizumi, Y. Ishido, 1998 .

Synthesis of nonadeca- and octadecaribonucleotides using the solid-phase phosphotriester with tetrahydropyranyl groups as the 2'-hydroxyl-protecting group

K. Miura, I. Hirao, Kimitsuna Watanabe, 1989 .

An intramolecular nucleophilic substitution of oxetanes

M. Yamaguchi, I. Hirao, 1985 .

Synthesis of Fused Oligoribonucleotides with Trideoxyribonucleotide Containing Phosphorothioate to Stabilize Against Nuclease Activity

K. Ikebukuro, K. Miura, I. Hirao, 1991 .

Synthesis of Oligoribonucleotides by the Hydroxybenzotriazole-Activated Phosphotriester/Dicyclohexylcarbodiimide System

K. Miura, I. Hirao, 1989 .

A ring opening reaction of oxetanes with lithium acetylides promoted by boron trifluoride etherate

M. Yamaguchi, I. Hirao, Yukio Nobayashi, 1984 .

Most compact hairpin-turn structure exerted by a short DNA fragment, d(GCGAAGC) in solution: an extraordinarily stable structure resistant to nucleases and heat.

K. Watanabe, G. Kawai, K. Miura, 1994, Nucleic acids research.

Studies of the Synthesis of Furan Compounds. XXVII. Derivatives of 3-(5-Nitro-2-furyl)acrylonitrile

I. Hirao, Y. Kato, 1972 .

Condensation of Anise Alcohol or Benzyl Alcohol with Aromatic Amines

I. Hirao, M. Hayashi, 1954 .

Synthesis of Oligoribonucleosides by the Hydroxybenzotriazole-Activated Phosphotriester/Dicyclohexylcarbodiimide System.

K. Miura, I. Hirao, 1990 .

Extraordinary stable structure of short single-stranded DNA fragments containing a specific base sequence: d(GCGAAAGC).

K. Watanabe, K. Miura, I. Hirao, 1989, Nucleic acids research.

High-Affinity DNA Aptamer Generation Targeting von Willebrand Factor A1-Domain by Genetic Alphabet Expansion for Systematic Evolution of Ligands by Exponential Enrichment Using Two Types of Libraries Composed of Five Different Bases.

I. Hirao, M. Kimoto, Ken-ichiro Matsunaga, 2017, Journal of the American Chemical Society.

Highly specific unnatural base pair systems as a third base pair for PCR amplification

Shigeyuki Yokoyama, Ichiro Hirao, Michiko Kimoto, 2011, Nucleic acids research.

Mass-spectral Fragmentation of 3-(5-Nitro-2-furyl)-1,2,4-triazoles

I. Hirao, Y. Kato, 1972 .

A synthesis of alkynyl azacycloalkanes by the coupling reaction of alkynyl boranes and lactams

M. Yamaguchi, I. Hirao, 1983 .

Evolving Aptamers with Unnatural Base Pairs

Ichiro Hirao, Michiko Kimoto, I. Hirao, 2017, Current protocols in chemical biology.

Solid phase synthesis of oligoribonucleotides by the phosphoramidite approach using 2'-O-1-(2-chloroethoxy)ethyl protection

K. Miura, H. Takaku, I. Hirao, 1991 .

Synthesis and Properties of an Initiation Codon Analog Consisting of 2′-O-Methyl Nucleotides

K. Miura, I. Hirao, M. Ishikawa, 1990 .

Site‐Specific Functional Labeling of Nucleic Acids by In Vitro Replication and Transcription using Unnatural Base Pair Systems

I. Hirao, M. Kimoto, Yasushi Hikida, 2013 .

A new unnatural base pair system between fluorophore and quencher base analogues for nucleic acid-based imaging technology.

S. Yokoyama, I. Hirao, M. Kimoto, 2010, Journal of the American Chemical Society.

An efficient unnatural base pair for a base-pair-expanded transcription system.

S. Yokoyama, I. Hirao, M. Kimoto, 2005, Journal of the American Chemical Society.

Higher-order structure of bovine mitochondrial tRNA(SerUGA): chemical modification and computer modeling.

K. Watanabe, G. Kawai, K. Miura, 1994, Nucleic acids research.

GNA trinucleotide loop sequences producing extraordinarily stable DNA minihairpins.

K Watanabe, K. Watanabe, G. Kawai, 1997, Biochemistry.

Structural features and properties of an extraordinarily stable hairpin-turn structure of d(GCGAAGC).

K. Watanabe, G. Kawai, K. Miura, 1993, Nucleic acids symposium series.

Extraordinarily stable mini-hairpins: electrophoretical and thermal properties of the various sequence variants of d(GCGAAAGC) and their effect on DNA sequencing.

K Watanabe, K. Watanabe, K. Miura, 1992, Nucleic acids research.

Site-specific fluorescent probing of RNA molecules by unnatural base-pair transcription for local structural conformation analysis

S. Yokoyama, I. Hirao, M. Kimoto, 2010, Nature Protocols.

Monitoring the site-specific incorporation of dual fluorophore-quencher base analogues for target DNA detection by an unnatural base pair system.

S. Yokoyama, I. Hirao, M. Kimoto, 2011, Organic & biomolecular chemistry.

Exploring the roles of nucleobase desolvation and shape complementarity during the misreplication of O(6)-methylguanine.

I. Hirao, A. Berdis, Delia Chavarria, 2011, Journal of molecular biology.

Chemical fidelity of an RNA polymerase ribozyme

Kyle V. Butler, P. Herdewijn, E. Kool, 2013 .

High-affinity five/six-letter DNA aptamers with superior specificity enabling the detection of dengue NS1 protein variants beyond the serotype identification

Y. Leo, S. Vasoo, I. Hirao, 2021, Nucleic acids research.

Anti-(Raf-1) RNA aptamers that inhibit Ras-induced Raf-1 activation.

S. Yokoyama, M. Shirouzu, S. Mizutani, 2002, European journal of biochemistry.

Unnatural base pairs for specific transcription

S. Yokoyama, I. Hirao, M. Kimoto, 2001, Proceedings of the National Academy of Sciences of the United States of America.

Unnatural base pair systems toward the expansion of the genetic alphabet in the central dogma

I. Hirao, M. Kimoto, Ichiro HIRAO, 2012, Proceedings of the Japan Academy. Series B, Physical and biological sciences.

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells.

S. Yokoyama, K. Sakamoto, D. Kiga, 2002, Nucleic acids research.

Genetic alphabet expansion biotechnology by creating unnatural base pairs.

K. Hamashima, I. Hirao, M. Kimoto, 2018, Current opinion in biotechnology.

Shifted positioning of the anticodon nucleotide residues of amber suppressor tRNA species by Escherichia coli arginyl-tRNA synthetase.

S. Yokoyama, K. Sakamoto, D. Kiga, 2001, European journal of biochemistry.

Studies on Thiophene Derivatives. VI: Synthesis of Thienylbutanolamines@@@チエニルブタノールアミン類の合成その1

I. Hirao, K. Mizunuma, M. Hayashi, 1954 .

The limits of specificity: An experimental analysis with RNA aptamers to MS2 coat protein variants

A. Ellington, D. Peabody, I. Hirao, 2004, Molecular diversity.

Expansion of the Genetic Alphabet in Nucleic Acids by Creating New Base Pairs

I. Hirao, M. Kimoto, 2010 .

RNA aptamers that specifically bind to the Ras‐binding domain of Raf‐1

S. Yokoyama, K. Sakamoto, M. Shirouzu, 1998, FEBS letters.

Site-specific incorporation of extra components into RNA by transcription using unnatural base pair systems.

I. Hirao, M. Kimoto, 2010, Methods in molecular biology.

Site-specific incorporation of functional components into RNA by transcription using unnatural base pair systems.

S. Yokoyama, I. Hirao, M. Kimoto, 2009, Nucleic acids symposium series.

Site-specific biotinylation of RNA molecules by transcription using unnatural base pairs

Shigeyuki Yokoyama, Kei Moriyama, Ichiro Hirao, 2005, Nucleic acids research.

RNA Aptamers That Bind to and Inhibit the Ribosome-inactivating Protein, Pepocin*

A. Ellington, S. Yokoyama, Y. Endo, 2000, The Journal of Biological Chemistry.

Interaction analysis between tmRNA and SmpB from Thermus thermophilus.

Gota Kawai, Akira Muto, Shigeyuki Yokoyama, 2005, Journal of biochemistry.

The Telomerization of Methyl Acetate and Methyl Cyanoacetate with Ethylene

I. Hirao, T. Fujimoto, 1974 .

Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry.

Takumi Ishizuka, I. Hirao, M. Kimoto, 2012, Chemical communications.

DNA aptamer generation by ExSELEX using genetic alphabet expansion with a mini-hairpin DNA stabilization method.

I. Hirao, M. Kimoto, Kyung Hyun Lee, 2017, Biochimie.

Uptake mechanisms of cell-internalizing nucleic acid aptamers for applications as pharmacological agents.

I. Hirao, M. Kimoto, S. Alamudi, 2021, RSC medicinal chemistry.

Studies on Acetylenic Alcohol and Its Derivatives. X: The Polymerization of Vinyl Pyrrolidone Catalyzed by Hydrogen Peroxide and Ammonia in Aqueous Solution@@@ビニルピロリドンの過酸化水素及びアンモニアによる水溶液重合について

I. Hirao, T. Otsu, S. Kikuchi, 1955 .

Studies on Acetylenic Alcohol and Its Derivatives. XI: Fractionation and Viscosity Measurement of Polyvinylpyrrolidone@@@ポリビニルピロリドンの分別と粘度測定結果について

I. Hirao, T. Otsu, S. Kikuchi, 1955 .

Studies of the Synthesis of Furan Compounds. XXVIII. Syntheses and Steric Configurations of 3-(5-Nitro-2-furyl)-2-(2-furyl)acrylonitrile and Its Bromine Derivatives

I. Hirao, Y. Kato, T. Kuboyama, 1972 .

Architecture of high-affinity unnatural-base DNA aptamers toward pharmaceutical applications

C. Hanson, H. Young, I. Hirao, 2015, Scientific Reports.

Re-creating the RNA world

Ichiro Hirao, A. Ellington, I. Hirao, 1995, Current Biology.

Studies of the Synthesis of Furan Compounds. XXIII. Cyclization Derivatives of 5-Nitro-2-furimidoylhydrazine

I. Hirao, Y. Kato, H. Tateishi, 1971 .

Dual Specificity of the Pyrimidine Analogue, 4-Methylpyridin-2-one, in DNA Replication

T. Ohtsuki, S. Yokoyama, I. Hirao, 2000 .

Genetic Alphabet Expansion by Unnatural Base Pair Creation and Its Application to High-Affinity DNA Aptamers

I. Hirao, M. Kimoto, Ken-ichiro Matsunaga, 2016 .

Synthesis of 3-(2-deoxy-β-d-ribofuranosyl)pyridin-2-one and 2-amino-6-(N,N-dimethylamino)-9-(2-deoxy-β-d-ribofuranosyl)purine derivatives for an unnatural base pair

S. Yokoyama, I. Hirao, M. Ishikawa, 2000 .

A quantitative, non-radioactive single-nucleotide insertion assay for analysis of DNA replication fidelity by using an automated DNA sequencer

S. Yokoyama, I. Hirao, M. Kimoto, 2004, Biotechnology Letters.

Synthesis of DNA Templates Containing the Fifth Base, 2-Amino-6-(dimethylamino)purine, for Specific Transcription Involving Unnatural Base Pairs

S. Yokoyama, I. Hirao, T. Nojima, 2001 .

In vitro selection of aptamers that bind to ribosome-inactivating toxins.

A. Ellington, S. Yokoyama, Y. Endo, 1997, Nucleic acids symposium series.

Ion Selectivities of Some Extracellular Viscous Polysaccharides from Red Algae

I. Hirao, Toshio Shimizu, A. Minakata, 1982 .

Landscapes for molecular evolution: Lessons from in vitro selection experiments with nucleic acids

Andrew D. Ellington, Ichiro Hirao, A. Ellington, 1997 .

DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair System.

I. Hirao, M. Kimoto, Ken-ichiro Matsunaga, 2016, Methods in molecular biology.

Unique hairpin structures occurring at the replication origin of phage G4 DNA.

K. Watanabe, K. Miura, I. Hirao, 1990, Biochimica et Biophysica Acta.

Post-ExSELEX stabilization of an unnatural-base DNA aptamer targeting VEGF165 toward pharmaceutical applications

Ichiro Hirao, Michiko Kimoto, I. Hirao, 2016, Nucleic acids research.

Carboxylation of Phenol Derivatives. XXI. The Formation Reaction of the Complex from Alkali Phenoxide and Carbon Dioxide

I. Hirao, T. Kito, 1973 .

Creation of unnatural base pairs for genetic alphabet expansion toward synthetic xenobiology.

K. Hamashima, I. Hirao, M. Kimoto, 2018, Current opinion in chemical biology.

High Fidelity, Efficiency and Functionalization of Ds-Px Unnatural Base Pairs in PCR Amplification for a Genetic Alphabet Expansion System.

Ichiro Hirao, Michiko Kimoto, Itaru Okamoto, 2016, ACS synthetic biology.

Dye-Conjugated Spinach RNA by Genetic Alphabet Expansion.

G. Kawai, I. Hirao, I. Okamoto, 2022, Chemistry.

PCR Amplification and Transcription for Site-Specific Labeling of Large RNA Molecules by a Two-Unnatural-Base-Pair System

S. Yokoyama, I. Hirao, M. Kimoto, 2012, Journal of nucleic acids.

Development of an unnatural base pair for efficient PCR amplification.

S. Yokoyama, I. Hirao, M. Kimoto, 2007, Nucleic Acids Symposium Series.

An unnatural base pair system for in vitro replication and transcription.

S. Yokoyama, T. Fujiwara, I. Hirao, 2006, Nucleic Acids Symposium Series.

Generation of high-affinity DNA aptamers using an expanded genetic alphabet

S. Yokoyama, I. Hirao, M. Kimoto, 2013, Nature Biotechnology.

Natural versus artificial creation of base pairs in DNA: origin of nucleobases from the perspectives of unnatural base pair studies.

I. Hirao, M. Kimoto, Rie Yamashige, 2012, Accounts of chemical research.

Exploring the roles of nucleobase desolvation and shape complementarity during the misreplication of O(6)-methylguanine.

I. Hirao, A. Berdis, Delia Chavarria, 2011, Journal of molecular biology.

Site-specific fluorescent labeling of RNA by a base-pair expanded transcription system.

S. Yokoyama, I. Hirao, M. Kimoto, 2004, Nucleic acids symposium series.

Stabilization of mRNA in an Escherichia coli cell‐free translation system

K. Miura, S. Yoshizawa, I. Hirao, 1993, FEBS letters.