D. Reeks

High-level periplasmic expression in Escherichia coli using a eukaryotic signal peptide: importance of codon usage at the 5' end of the coding sequence.

P. Stephens, D. Humphreys, M. Sehdev, 2000, Protein expression and purification.

Efficient site specific removal of a C-terminal FLAG fusion from a Fab' using copper(II) ion catalysed protein cleavage.

P. Stephens, D. Humphreys, B. Smith, 1999, Protein engineering.

Formation of dimeric Fabs in Escherichia coli: effect of hinge size and isotype, presence of interchain disulphide bond, Fab' expression levels, tail piece sequences and growth conditions.

P. Stephens, D. Humphreys, A. P. Chapman, 1997, Journal of immunological methods.

A plasmid system for optimization of Fab' production in Escherichia coli: importance of balance of heavy chain and light chain synthesis.

A. Lawson, D. Humphreys, M. Sehdev, 2002, Protein expression and purification.

F(ab')2 molecules made from Escherichia coli produced Fab' with hinge sequences conferring increased serum survival in an animal model.

A. Suitters, P. Stephens, D. Humphreys, 1998, Journal of immunological methods.