J. Boulain

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Molecular evolution of snake toxins: is the functional diversity of snake toxins associated with a mechanism of accelerated evolution?

Y. Shimohigashi, S. Zinn-Justin, A. Ménez, 1998, Progress in nucleic acid research and molecular biology.

Recombinant Colorimetric Antibodies: Construction and Characterization of a Bifunctional F(ab)2/Alkaline Phosphatase Conjugate Produced in Escherichia coli

A. Ménez, D. Gillet, E. Lajeunesse, 1993, Bio/Technology.

Neurotoxin-specific immunoglobulins accelerate dissociation of the neurotoxin-acetylcholine receptor complex.

A. Ménez, J. Boulain, 1982, Science.

Further evidence showing that neurotoxin-acetylcholine receptor dissociation is accelerated by monoclonal neurotoxin-specific immunoglobulin.

A. Ménez, P. Fromageot, J. Boulain, 1985, Molecular immunology.

High-level production and isotope labeling of snake neurotoxins, disulfide-rich proteins.

S. Zinn-Justin, A. Ménez, E. Lajeunesse, 1997, Protein expression and purification.

Probing the topology of a bacterial membrane protein by genetic insertion of a foreign epitope; expression at the cell surface.

M. Hofnung, A. Charbit, A. Ryter, 1986, The EMBO journal.

Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork.

Hongtao Lei, F. Ducancel, J. Boulain, 2012, Analytica chimica acta.

Enzyme immunoassay using a rat prolactin-alkaline phosphatase recombinant tracer.

A. Ménez, E. Ezan, D. Gillet, 1993, Analytical chemistry.

Identification of snake species by toxin mass fingerprinting of their venoms.

R. Stöcklin, D. Mebs, J. Boulain, 2000, Methods in molecular biology.

Engineering of a recombinant colorimetric fusion protein for immunodiagnosis of insulin.

A. Ménez, J. Boulain, L. Bellanger, 1996, Journal of immunological methods.

Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection.

L. Cattolico, A. Ménez, B. Maillère, 1995, Journal of immunological methods.

POSTSYNAPTICALLY-ACTING TOXINS AND PROTEINS WITH PHOSPHOLIPASE STRUCTURE FROM SNAKE VENOMS: COMPLETE AMINO ACID SEQUENCES DEDUCED FROM cDNAs and PRODUCTION OF A TOXIN WITH STAPHYLOCOCCAL PROTEIN A GENE FUSION VECTOR

A. Ménez, F. Ducancel, J. Boulain, 1989 .

Cloning and sequence analysis of cDNAs encoding precursors of sarafotoxins. Evidence for an unusual "rosary-type" organization.

Z. Wollberg, A. Ménez, E. Lajeunesse, 1993, The Journal of biological chemistry.

Cloning and Sequence Analysis of cDNAs Encoding Precursors of Sarafotoxins

A. Ménez, E. Lajeunesse, F. Ducancel, 1993 .

Regional and accelerated molecular evolution in group I snake venom gland phospholipase A2 isozymes.

Y. Chuman, Y. Shimohigashi, A. Ménez, 2000, Toxicon : official journal of the International Society on Toxinology.

Analysis of cDNAs encoding the two subunits of crotoxin, a phospholipase A2 neurotoxin from rattlesnake venom: the acidic non enzymatic subunit derives from a phospholipase A2-like precursor.

A. Ménez, C. Bouchier, J. Boulain, 1991, Biochimica et biophysica acta.

Amino acid sequence of a muscarinic toxin deduced from the cDNA nucleotide sequence.

A. Ménez, A. Harvey, F. Ducancel, 1991, Toxicon : official journal of the International Society on Toxinology.

Nucleotide sequence encoding a 'synergistic-like' protein from the venom glands of Dendroaspis angusticeps.

A. Ménez, A. Harvey, F. Ducancel, 1990, Nucleic acids research.

Nucleotide sequence and structure analysis of cDNAs encoding short-chain neurotoxins from venom glands of a sea snake (Aipysurus laevis).

A. Ménez, F. Ducancel, J. Boulain, 1990, Toxicon : official journal of the International Society on Toxinology.

Cloning and sequencing of cDNAs encoding the two subunits of Crotoxin.

A. Ménez, C. Bouchier, F. Ducancel, 1988, Nucleic acids research.

Complete amino acid sequence of a PLA2 from the tiger snake Notechis scutatus scutatus as deduced from a complementary DNA.

A. Ménez, C. Bouchier, F. Ducancel, 1988, Nucleic acids research.

Sequence analysis of a cDNA encoding a PLA2 from the sea-snake Aipysurus laevis.

A. Ménez, C. Bouchier, F. Ducancel, 1988, Nucleic acids research.

Sequence of a cDNA encoding a snake venom phospholipase A2.

A. Ménez, F. Ducancel, J. Boulain, 1987, Nucleic acids research.

On the site by which α‐dendrotoxin binds to voltage‐dependent potassium channels: Site‐directed mutagenesis reveals that the lysine triplet 28–30 is not essential for binding

A. Ménez, H. Vatanpour, E. Lajeunesse, 1994, FEBS letters.

Three‐dimensional crystal structure of recombinant erabutoxin a at 2.0 Å resolution

A. Ménez, A. Ducruix, R. Ménez, 1994, FEBS letters.

A monoclonal antibody which recognized the functional site of snake neurotoxins and which neutralizes all short‐chain variants

A. Ménez, P. Fromageot, J. Boulain, 1986, FEBS letters.

Comparison of the "toxic" and antigenic regions in toxin α isolated from Naja nigricollis venom

A. Ménez, P. Fromageot, G. Faure, 1982 .

Sarafotoxins:Cloning of mRNAs Encoding Sarafotoxin Precursors

A. Ménez, F. Ducancel, J. Boulain, 2000 .

Recombinant technology in the preparation of immunogen and enzymatic tracer. Application to the development of an enzyme immunoassay for rat prolactin.

A. Ménez, E. Ezan, D. Gillet, 1994, Journal of immunological methods.

Molecular complex for targeting antigens to antigen-presenting cells and its applications for vaccination

J. Boulain, A. Savatier, M. Léonetti, 2010 .

The low molecular weight protein which co-purifies with alpha-latrotoxin is structurally related to crustacean hyperglycemic hormones.

E. Grishin, A. Ménez, P. Ripoche, 1994, The Journal of biological chemistry.

Increasing immunogenicity of antigens fused to Ig-binding proteins by cell surface targeting.

A. Ménez, S. Leroy, F. Ducancel, 1998, Journal of immunology.

Precursors of vertebrate peptide antibiotics dermaseptin b and adenoregulin have extensive sequence identities with precursors of opioid peptides dermorphin, dermenkephalin, and deltorphins.

A. Ménez, A. Mor, P. Nicolas, 1994, The Journal of biological chemistry.

Heparan sulfates targeting increases MHC class I- and MHC class II-restricted antigen presentation and CD8(+) T-cell response.

C. Sedlik, E. Piaggio, J. Boulain, 2016, Vaccine.

Genetic engineering of snake toxins. Role of invariant residues in the structural and functional properties of a curaremimetic toxin, as probed by site-directed mutagenesis.

S. Zinn-Justin, A. Ménez, F. Ducancel, 1993, The Journal of biological chemistry.

Mutants with higher stability and specific activity from a single thermosensitive variant of T7 RNA polymerase.

E. Stura, F. Ducancel, J. Boulain, 2013, Protein engineering, design & selection : PEDS.

The interaction of neurotoxin derivatives with either acetylcholine receptor or a monoclonal antibody. An electron-spin-resonance study.

A. Ménez, A. Rousselet, G. Faure, 1984, European journal of biochemistry.

Production of recombinant notechis 11'2L, an enzymatically active mutant of a phospholipase A2 from Notechis scutatus scutatus venom, as directly generated by cleavage of a fusion protein produced in Escherichia coli.

A. Ménez, F. Ducancel, J. Boulain, 1993, European journal of biochemistry.

Role of indole and amino groups in the structure and function of Naja nigricollis toxin alpha.

P Fromageot, A. Ménez, P. Fromageot, 1983, Biochemistry.

Neutralizing monoclonal antibody specific for Naja nigricollis toxin alpha: preparation, characterization, and localization of the antigenic binding site.

A. Ménez, P. Fromageot, G. Faure, 1982, Biochemistry.

Mutagenesis by random linker insertion into the lamB gene of Escherichia coli K12

M. Hofnung, A. Charbit, J. Boulain, 1986, Molecular and General Genetics MGG.

Artificial evolution of an enzyme active site: structural studies of three highly active mutants of Escherichia coli alkaline phosphatase.

O. Bulgakov, A. Ménez, E. Lajeunesse, 2002, Journal of molecular biology.

The Functional Architecture of an Acetylcholine Receptor-mimicking Antibody*

S. Zinn-Justin, A. Ménez, K. Merienne, 1997, The Journal of Biological Chemistry.

Notechis 11′2, a non-toxic phospholipase A2 from the venom of Notechis scutatus scutatus

A. Ménez, F. Tesson, C. Bouchier, 1991 .

Recombinant single-chain Fv antibody fragment-alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization.

J. Guesdon, J. Boulain, D. Chevrier, 1999, Journal of immunological methods.

Expression of a poliovirus neutralization epitope at the surface of recombinant bacteria: first immunization results.

M. Hofnung, A. Charbit, S. van der Werf, 1988, Annales de l'Institut Pasteur. Microbiology.

A recombinant insect-specific alpha-toxin of Buthus occitanus tunetanus scorpion confers protection against homologous mammal toxins.

A. Ménez, H. Karoui, F. Ducancel, 1996, European journal of biochemistry.

Dual expression system suitable for high-throughput fluorescence-based screening and production of soluble proteins.

S. Zinn-Justin, A. Ménez, J. Charbonnier, 2005, Journal of proteome research.

BgK, a disulfide-containing sea anemone toxin blocking K+ channels, can be produced in Escherichia coli cytoplasm as a functional tagged protein.

G. Mourier, A. Ménez, J. Boulain, 2004, Protein expression and purification.

Molecular cloning of a cDNA encoding the precursor of adenoregulin from frog skin. Relationships with the vertebrate defensive peptides, dermaseptins.

A. Ménez, P. Nicolas, M. Amiche, 1993, Biochemical and biophysical research communications.

The antigenic structure of a cobra neurotoxin : Molecular mechanisms associated with neutralization by monoclonal specific immunoglobulins

A. Ménez, J. Boulain, 1983 .

Expression of recombinant toxin-like protein from a scorpion cDNA library

A. Ménez, H. Karoui, F. Ducancel, 1995 .

hybrid proteins between an extracytoplasmic enzyme and another protein, their preparation process and their application as a diagnostic agent

A. Ménez, P. Boquet, J. Boulain, 1989 .

Genetic approaches to the study and use of proteins: random point mutations and random linker insertions

M. Hofnung, W. Saurin, P. Martineau, 1988 .

Direct expression in E. coli of a functionally active protein A--snake toxin fusion protein.

A. Ménez, F. Ducancel, J. Boulain, 1989, Protein engineering.

Expression of recombinant alkaline phosphatase conjugates in Escherichia coli.

F. Ducancel, J. Boulain, 2004, Methods in molecular biology.

Insertion of a disulfide-containing neurotoxin into E. coli alkaline phosphatase: the hybrid retains both biological activities.

A. Ménez, D. Gillet, F. Ducancel, 1992, Protein engineering.

Structure-based secondary structure-independent approach to design protein ligands: Application to the design of Kv1.2 potassium channel blockers.

A Joly, P Cuniasse, M. Lefranc, 2006, Journal of the American Chemical Society.

Molecular and Structural Basis of the Specificity of a Neutralizing Acetylcholine Receptor-mimicking Antibody, Using Combined Mutational and Molecular Modeling Analyses*

S. Zinn-Justin, A. Ménez, K. Merienne, 2000, The Journal of Biological Chemistry.

“Three-Fingered” Toxins from Hydrophid and Elapid Snakes: Artificial Procedures to Overproduce Wild-Type and Mutated Curaremimetic Toxins

G. Mourier, A. Ménez, F. Ducancel, 2000 .

Complete amino-acid sequence of the beta-subunit of VTX from venom of the stonefish (Synanceia verrucosa) as identified from cDNA cloning experiments.

A. Ménez, T. Ogawa, F. Ducancel, 1997, Biochimica et biophysica acta.

Mimicry between Receptors and Antibodies

S. Zinn-Justin, A. Ménez, F. Ducancel, 1996, The Journal of Biological Chemistry.

Improving Escherichia coli Alkaline Phosphatase Efficacy by Additional Mutations inside and outside the Catalytic Pocket

L. Cattolico, A. Ménez, E. Lajeunesse, 2001, Chembiochem : a European journal of chemical biology.

Do Structural Deviations between Toxins Adopting the Same Fold Reflect Functional Differences?*

A. Ménez, F. Ducancel, M. Le Du, 2000, The Journal of Biological Chemistry.

Genetic Engineering of Snake Toxins

A. Ménez, F. Ducancel, J. Boulain, 1995, The Journal of Biological Chemistry.

Stability of a structural scaffold upon activity transfer: X-ray structure of a three fingers chimeric protein.

A. Ménez, F. Ducancel, M. Le Du, 2000, Journal of molecular biology.