A. Knappik

发表

Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides.

A. Plückthun, A. Honegger, B. Virnekäs, 2000, Journal of molecular biology.

Microarray of recombinant antibodies using a streptavidin sensor surface self-assembled onto a gold layer.

H. Hug, P. Pavlícková, D. Kambhampati, 2003, BioTechniques.

An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments.

A. Plückthun, A. Knappik, A. Plückthun, 1994, BioTechniques.

Periplasmic Expression of SpyTagged Antibody Fragments Enables Rapid Modular Antibody Assembly

A. Knappik, Christian Hentrich, Sarah-Jane Kellmann, 2020, bioRxiv.

Animal-free alternatives and the antibody iceberg

A. Plückthun, S. Dübel, A. Bradbury, 2020, Nature Biotechnology.

Isolation and characterization of selective and potent human Fab inhibitors directed to the active-site region of the two-component NS2B-NS3 proteinase of West Nile virus.

A. Strongin, B. Stec, Ilian A Radichev, 2010, The Biochemical journal.

Isolation and comparative characterization of Ki-67 equivalent antibodies from the HuCAL® phage display library

H. Merz, A. Knappik, C. Frisch, 2006, Biological chemistry.

Crystallization and preliminary X-ray crystallographic analysis of the sclerostin-neutralizing Fab AbD09097.

T. Mueller, A. Knappik, C. Frisch, 2015, Acta crystallographica. Section F, Structural biology communications.

Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin.

T. Mueller, J. Back, A. Knappik, 2012, Protein engineering, design & selection : PEDS.

Recombinant monoclonal antibodies.

A. Knappik, M. Donzeau, 2007, Methods in molecular biology.

Development of Recombinant Human IgA for Anticardiolipin Antibodies Assay Standardization

A. Knappik, C. Frisch, F. Bonelli, 2009, Annals of the New York Academy of Sciences.

Recombinant Antibody Expression and Purification

R. Brundiers, A. Knappik, 2009 .

Off-rate screening for selection of high-affinity anti-drug antibodies.

A. Knappik, C. Frisch, Stefan Harth, 2013, Analytical biochemistry.

SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology

A. Knappik, Christian Hentrich, Sarah-Jane Kellmann, 2022, bioRxiv.

High-Affinity Recombinant Antibody Fragments (Fabs) Can Be Applied in Peptide Enrichment Immuno-MRM Assays

Jeffrey R. Whiteaker, A. Paulovich, A. Knappik, 2014, Journal of proteome research.

Dimeric 3‐Phosphoglycerate Kinases from Hyperthermophilic Archaea

R. Hensel, D. Hess, A. Knappik, 1995 .

Antibodies for proteomic research: Comparison of traditional immunization with recombinant antibody technology

H. Koga, K. Shimada, A. Knappik, 2006, Proteomics.

Anti-Sclerostin Antibody Inhibits Internalization of Sclerostin and Sclerostin-Mediated Antagonism of Wnt/LRP6 Signaling

T. Mueller, Juan Zhang, David J J de Gorter, 2013, PloS one.

A novel reverse transduction adenoviral array for the functional analysis of shRNA libraries

M. Kubbutat, R. Brundiers, T. Joos, 2008, BMC Genomics.

Human Combinatorial Fab Library Yielding Specific and Functional Antibodies against the Human Fibroblast Growth Factor Receptor 3*

Rivka Adar, Avner Yayon, R. Adar, 2003, Journal of Biological Chemistry.

Direct kinetic fingerprinting and digital counting of single protein molecules

M. Tewari, N. Walter, E. Sandford, 2020, Proceedings of the National Academy of Sciences.

Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display

Andreas Plückthun, Christiane Schaffitzel, A. Plückthun, 2000, Nature Biotechnology.

The sclerostin-neutralizing antibody AbD09097 recognizes an epitope adjacent to sclerostin's binding site for the Wnt co-receptor LRP6

P. Schmieder, T. Mueller, J. Back, 2016, Open Biology.

Engineered turns of a recombinant antibody improve its in vivo folding.

A. Plückthun, A. Knappik, A Plückthun, 1995, Protein engineering.

High-throughput generation and engineering of recombinant human antibodies.

Manqiu Cao, M. Tesar, R. Schier, 2001, Journal of immunological methods.

Animal-derived-antibody generation faces strict reform in accordance with European Union policy on animal use

A. Plückthun, S. Dübel, A. Bradbury, 2020, Nature Methods.

Reproducibility: bypass animals for antibody production

A. Plückthun, S. Dübel, A. Bradbury, 2020, Nature.

Periplasmic expression of SpyTagged antibody fragments enables rapid modular antibody assembly.

A. Knappik, Christian Hentrich, Sarah-Jane Kellmann, 2021, Cell chemical biology.

Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics

A. Knappik, C. Frisch, C. Loew, 2018, mAbs.

The Effect of Folding Catalysts on the In Vivo Folding Process of Different Antibody Fragments Expressed in Escherichia coli

A. Plückthun, A. Knappik, C. Krebber, 1993, Bio/Technology.

Animal- versus in vitro-derived antibodies: avoiding the extremes

A. Plückthun, S. Dübel, A. Bradbury, 2021, mAbs.

Selection of vimentin-specific antibodies from the HuCAL® phage display library by subtractive panning on formalin-fixed, paraffin-embedded tissue

H. Merz, A. Knappik, C. Frisch, 2007, Biological chemistry.

Strategies for Recombinant Antibody Library Synthesis: An Advanced Source for Immunoglobulins in Environmental Analysis

A. Knappik, K. Kramer, 1998 .

Dimeric 3-phosphoglycerate kinases from hyperthermophilic Archaea. Cloning, sequencing and expression of the 3-phosphoglycerate kinase gene of Pyrococcus woesei in Escherichia coli and characterization of the protein. Structural and functional comparison with the 3-phosphoglycerate kinase of Methano

R. Hensel, D. Hess, A. Knappik, 1995, European journal of biochemistry.