M. Kimoto

发表

Generation of high-affinity DNA aptamers using an expanded genetic alphabet

S. Yokoyama, I. Hirao, M. Kimoto, 2013, Nature Biotechnology.

Sanger Gap Sequencing for Genetic Alphabet Expansion of DNA.

I. Hirao, M. Kimoto, Si Hui Gabriella Soh, 2020, Chembiochem : a European journal of chemical biology.

Cognate base‐pair selectivity of hydrophobic unnatural bases in DNA ligation by T4 DNA ligase

I. Hirao, I. Okamoto, M. Kimoto, 2020, Biopolymers.

DNA Sequencing Method Including Unnatural Bases for DNA Aptamer Generation by Genetic Alphabet Expansion.

K. Hamashima, I. Hirao, M. Kimoto, 2019, ACS synthetic biology.

Genetic Alphabet Expansion Provides Versatile Specificities and Activities of Unnatural-Base DNA Aptamers Targeting Cancer Cells

I. Hirao, M. Kimoto, K. Futami, 2018, Molecular therapy. Nucleic acids.

Unnatural base pairs between 2- and 6-substituted purines and 2-oxo(1H)pyridine for expansion of the genetic alphabet.

S. Yokoyama, T. Fujiwara, I. Hirao, 2004, Bioorganic & medicinal chemistry letters.

A unique unnatural base pair between a C analogue, pseudoisocytosine, and an A analogue, 6-methoxypurine, in replication.

S. Yokoyama, I. Hirao, M. Kimoto, 2002, Bioorganic & medicinal chemistry letters.

Synthesis of 6-(2-thienyl)purine nucleoside derivatives that form unnatural base pairs with pyridin-2-one nucleosides.

S. Yokoyama, H. Sugiyama, T. Fujiwara, 2001, Bioorganic & medicinal chemistry letters.

Molecular affinity rulers: systematic evaluation of DNA aptamers for their applicabilities in ELISA

I. Hirao, M. Kimoto, Yun Wei Shermane Lim, 2019, Nucleic acids research.

Success probability of high-affinity DNA aptamer generation by genetic alphabet expansion

Y. S. Tan, I. Hirao, M. Kimoto, 2022, bioRxiv.

Competitive ELISA for a serologic test to detect dengue serotype-specific anti-NS1 IgGs using high-affinity UB-DNA aptamers

Y. Leo, S. Vasoo, I. Hirao, 2021, Scientific Reports.

Unnatural base pairs between 2-amino-6-(2-thienyl)purine and the complementary bases.

T. Ohtsuki, S. Yokoyama, T. Fujiwara, 2000, Nucleic acids symposium series.

Genetic Code Engineering by Natural and Unnatural Base Pair Systems for the Site-Specific Incorporation of Non-Standard Amino Acids Into Proteins

I. Hirao, M. Kimoto, 2022, Frontiers in Molecular Biosciences.

Site-Specific Incorporation of Functional Components into RNA by an Unnatural Base Pair Transcription System

I. Hirao, M. Kimoto, R. Kawai, 2012, Molecules.

A two-unnatural-base-pair system toward the expansion of the genetic code.

S. Yokoyama, T. Fujiwara, I. Hirao, 2004, Journal of the American Chemical Society.

An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules

Shigeyuki Yokoyama, Ichiro Hirao, Michiko Kimoto, 2008, Nucleic acids research.

Visual Detection of Amplified DNA by Polymerase Chain Reaction Using a Genetic Alphabet Expansion System.

I. Hirao, M. Kimoto, Rie Yamashige, 2018, Journal of the American Chemical Society.

Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants.

A. Ellington, I. Hirao, M. Kimoto, 2017, Chemical communications.

Structural Basis for Expansion of the Genetic Alphabet with an Artificial Nucleobase Pair.

K. Diederichs, A. Marx, I. Hirao, 2017, Angewandte Chemie.

Unnatural base pair systems for sensing and diagnostic applications

R. Cox, I. Hirao, M. Kimoto, 2011, Expert review of molecular diagnostics.

A unique fluorescent base analogue for the expansion of the genetic alphabet.

S. Yokoyama, I. Hirao, M. Kimoto, 2010, Journal of the American Chemical Society.

An efficient unnatural base pair for PCR amplification.

S. Yokoyama, I. Hirao, M. Kimoto, 2007, Journal of the American Chemical Society.

Site-specific fluorescent labeling of RNA molecules by specific transcription using unnatural base pairs.

S. Yokoyama, S. Ikeda, Masayuki Endo, 2005, Journal of the American Chemical Society.

Characterization of fluorescent, unnatural base pairs

S. Yokoyama, I. Hirao, M. Kimoto, 2007 .

An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA

Yoko Harada, Tsuyoshi Fujiwara, Shigeyuki Yokoyama, 2006, Nature Methods.

An unnatural hydrophobic base, 4-propynylpyrrole-2-carbaldehyde, as an efficient pairing partner of 9-methylimidazo[(4,5)-b]pyridine.

S. Yokoyama, I. Hirao, M. Kimoto, 2003, Bioorganic & medicinal chemistry letters.

An unnatural hydrophobic base pair with shape complementarity between pyrrole-2-carbaldehyde and 9-methylimidazo[(4,5)-b]pyridine.

Shigeyuki Yokoyama, Ichiro Hirao, Michiko Kimoto, 2003, Journal of the American Chemical Society.

Genetic alphabet expansion technology by creating unnatural base pairs.

Ichiro Hirao, Michiko Kimoto, I. Hirao, 2020, Chemical Society reviews.

Natural versus artificial creation of base pairs in DNA: origin of nucleobases from the perspectives of unnatural base pair studies.

Ichiro Hirao, Michiko Kimoto, I. Hirao, 2012, Accounts of chemical research.

Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase

Dong Wang, J. Chong, J. Oh, 2023, Nature Communications.

Fluorescent probing for RNA molecules by an unnatural base-pair system

S. Yokoyama, I. Hirao, M. Kimoto, 2007, Nucleic acids research.

Site-specific labeling of RNA by combining genetic alphabet expansion transcription and copper-free click chemistry

I. Hirao, M. Kimoto, T. Someya, 2015, Nucleic acids research.

Cytostatic evaluations of nucleoside analogs related to unnatural base pairs for a genetic expansion system.

S. Yokoyama, I. Hirao, M. Kimoto, 2007, Bioorganic & medicinal chemistry letters.

Site-specific incorporation of a photo-crosslinking component into RNA by T7 transcription mediated by unnatural base pairs.

S. Yokoyama, Masayuki Endo, I. Hirao, 2004, Chemistry & biology.

High-Affinity DNA Aptamer Generation Targeting von Willebrand Factor A1-Domain by Genetic Alphabet Expansion for Systematic Evolution of Ligands by Exponential Enrichment Using Two Types of Libraries Composed of Five Different Bases.

I. Hirao, M. Kimoto, Ken-ichiro Matsunaga, 2017, Journal of the American Chemical Society.

Highly specific unnatural base pair systems as a third base pair for PCR amplification

Shigeyuki Yokoyama, Ichiro Hirao, Michiko Kimoto, 2011, Nucleic acids research.

Evolving Aptamers with Unnatural Base Pairs

Ichiro Hirao, Michiko Kimoto, I. Hirao, 2017, Current protocols in chemical biology.

Site‐Specific Functional Labeling of Nucleic Acids by In Vitro Replication and Transcription using Unnatural Base Pair Systems

I. Hirao, M. Kimoto, Yasushi Hikida, 2013 .

A new unnatural base pair system between fluorophore and quencher base analogues for nucleic acid-based imaging technology.

S. Yokoyama, I. Hirao, M. Kimoto, 2010, Journal of the American Chemical Society.

An efficient unnatural base pair for a base-pair-expanded transcription system.

S. Yokoyama, I. Hirao, M. Kimoto, 2005, Journal of the American Chemical Society.

Site-specific fluorescent probing of RNA molecules by unnatural base-pair transcription for local structural conformation analysis

S. Yokoyama, I. Hirao, M. Kimoto, 2010, Nature Protocols.

Monitoring the site-specific incorporation of dual fluorophore-quencher base analogues for target DNA detection by an unnatural base pair system.

S. Yokoyama, I. Hirao, M. Kimoto, 2011, Organic & biomolecular chemistry.

Chemical fidelity of an RNA polymerase ribozyme

Kyle V. Butler, P. Herdewijn, E. Kool, 2013 .

Molecular Technology Toward Expansion of Nucleic Acid Functionality

M. Kimoto, K. Kawai, 2019, Molecular Technology.

High-affinity five/six-letter DNA aptamers with superior specificity enabling the detection of dengue NS1 protein variants beyond the serotype identification

Y. Leo, S. Vasoo, I. Hirao, 2021, Nucleic acids research.

Anti-(Raf-1) RNA aptamers that inhibit Ras-induced Raf-1 activation.

S. Yokoyama, M. Shirouzu, S. Mizutani, 2002, European journal of biochemistry.

Unnatural base pairs for specific transcription

S. Yokoyama, I. Hirao, M. Kimoto, 2001, Proceedings of the National Academy of Sciences of the United States of America.

Unnatural base pair systems toward the expansion of the genetic alphabet in the central dogma

I. Hirao, M. Kimoto, Ichiro HIRAO, 2012, Proceedings of the Japan Academy. Series B, Physical and biological sciences.

Genetic alphabet expansion biotechnology by creating unnatural base pairs.

K. Hamashima, I. Hirao, M. Kimoto, 2018, Current opinion in biotechnology.

Expansion of the Genetic Alphabet in Nucleic Acids by Creating New Base Pairs

I. Hirao, M. Kimoto, 2010 .

RNA aptamers that specifically bind to the Ras‐binding domain of Raf‐1

S. Yokoyama, K. Sakamoto, M. Shirouzu, 1998, FEBS letters.

Site-specific incorporation of extra components into RNA by transcription using unnatural base pair systems.

I. Hirao, M. Kimoto, 2010, Methods in molecular biology.

Site-specific incorporation of functional components into RNA by transcription using unnatural base pair systems.

S. Yokoyama, I. Hirao, M. Kimoto, 2009, Nucleic acids symposium series.

Site-specific biotinylation of RNA molecules by transcription using unnatural base pairs

Shigeyuki Yokoyama, Kei Moriyama, Ichiro Hirao, 2005, Nucleic acids research.

Interaction analysis between tmRNA and SmpB from Thermus thermophilus.

Gota Kawai, Akira Muto, Shigeyuki Yokoyama, 2005, Journal of biochemistry.

Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry.

Takumi Ishizuka, I. Hirao, M. Kimoto, 2012, Chemical communications.

DNA aptamer generation by ExSELEX using genetic alphabet expansion with a mini-hairpin DNA stabilization method.

I. Hirao, M. Kimoto, Kyung Hyun Lee, 2017, Biochimie.

Uptake mechanisms of cell-internalizing nucleic acid aptamers for applications as pharmacological agents.

I. Hirao, M. Kimoto, S. Alamudi, 2021, RSC medicinal chemistry.

Architecture of high-affinity unnatural-base DNA aptamers toward pharmaceutical applications

C. Hanson, H. Young, I. Hirao, 2015, Scientific Reports.

Genetic Alphabet Expansion by Unnatural Base Pair Creation and Its Application to High-Affinity DNA Aptamers

I. Hirao, M. Kimoto, Ken-ichiro Matsunaga, 2016 .

A quantitative, non-radioactive single-nucleotide insertion assay for analysis of DNA replication fidelity by using an automated DNA sequencer

S. Yokoyama, I. Hirao, M. Kimoto, 2004, Biotechnology Letters.

DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair System.

I. Hirao, M. Kimoto, Ken-ichiro Matsunaga, 2016, Methods in molecular biology.

Post-ExSELEX stabilization of an unnatural-base DNA aptamer targeting VEGF165 toward pharmaceutical applications

Ichiro Hirao, Michiko Kimoto, I. Hirao, 2016, Nucleic acids research.

Creation of unnatural base pairs for genetic alphabet expansion toward synthetic xenobiology.

K. Hamashima, I. Hirao, M. Kimoto, 2018, Current opinion in chemical biology.

High Fidelity, Efficiency and Functionalization of Ds-Px Unnatural Base Pairs in PCR Amplification for a Genetic Alphabet Expansion System.

Ichiro Hirao, Michiko Kimoto, Itaru Okamoto, 2016, ACS synthetic biology.

Dye-Conjugated Spinach RNA by Genetic Alphabet Expansion.

G. Kawai, I. Hirao, I. Okamoto, 2022, Chemistry.

PCR Amplification and Transcription for Site-Specific Labeling of Large RNA Molecules by a Two-Unnatural-Base-Pair System

S. Yokoyama, I. Hirao, M. Kimoto, 2012, Journal of nucleic acids.

Development of an unnatural base pair for efficient PCR amplification.

S. Yokoyama, I. Hirao, M. Kimoto, 2007, Nucleic Acids Symposium Series.

An unnatural base pair system for in vitro replication and transcription.

S. Yokoyama, T. Fujiwara, I. Hirao, 2006, Nucleic Acids Symposium Series.

Strict Interactions of Fifth Letters, Hydrophobic Unnatural Bases, in XenoAptamers with Target Proteins

M. Kimoto, Ken-ichiro Matsunaga, Hui Pen Tan, 2023, Journal of the American Chemical Society.

Generation of high-affinity DNA aptamers using an expanded genetic alphabet

S. Yokoyama, I. Hirao, M. Kimoto, 2013, Nature Biotechnology.

Natural versus artificial creation of base pairs in DNA: origin of nucleobases from the perspectives of unnatural base pair studies.

I. Hirao, M. Kimoto, Rie Yamashige, 2012, Accounts of chemical research.

Site-specific fluorescent labeling of RNA by a base-pair expanded transcription system.

S. Yokoyama, I. Hirao, M. Kimoto, 2004, Nucleic acids symposium series.